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  • rad9
  • Intramolecular binding of the rad9 C-terminus in the checkpoint clamp Rad9-Hus1-Rad1 is closely linked with its DNA binding. (nih.gov)
  • We measured the bystander response of Mrad9 +/+ and Mrad9 −/− mouse embryonic stem cells, as well as human H1299 cells with inherent or RNA interference-mediated reduced RAD9 levels after exposure to 1 Gy α particles, by scoring chromosomal aberrations and micronuclei formation, respectively. (springer.com)
  • In addition, we found that H1299 cells with reduced RAD9 protein levels showed a higher frequency of radiation induced bystander micronuclei formation, compared with parental cells containing inherent levels of RAD9. (springer.com)
  • Cell cycle checkpoint control protein RAD9 influences the cellular response to both direct and bystander radiation exposure. (springer.com)
  • subunits
  • These subunits induce intracellular responses that can be highly polarized when a cell experiences a gradient of chemoattractant. (jove.com)
  • Su, Changqing 2018-02-01 00:00:00 Protein phosphatase 2, regulatory subunit B (B56), alpha isoform (PPP2R5A) is one of the regulatory subunits of the protein phosphatase 2A (PP2A), which is a major member of protein serine/threonine phosphatases in cells. (deepdyve.com)
  • homeostasis
  • DNA damage is a vital challenge to cell homeostasis. (jcmtjournal.com)
  • Thrombopoietin, essential for megakaryopoiesis and very important for hematopoietic stem cell (HSC) homeostasis, has also been found to promote DNA repair in a highly selective manner. (frontiersin.org)
  • tumor
  • Activation of the cellular DNA damage response (DDR) is an important determinant of cell sensitivity to cisplatin and other chemotherapeutic drugs that eliminate tumor cells through induction of DNA damage. (aacrjournals.org)
  • Biological approaches exploiting differences in cellular responses to RT between tumor and normal cells are desirable to specifically radiosensitize tumor cells and protect normal cells. (frontiersin.org)
  • Coculture experiments, designed to mimic critical interactions between tumor and brain microvascular endothelial cells, showed that specifically increasing GRK3 expression reduced the trophic effect of endothelial cells on tumor cells. (aacrjournals.org)
  • meiotic
  • We are interested in identifying the activities responsible for the formation and resolution of these intermediates, and the regulatory circuits that integrate these processes with the meiotic cell cycle. (cancer.gov)
  • cellular
  • 7 - 9 ] The inactive NF-κB is present in the cytoplasm in most cell types and it can be activated by a variety of extra-cellular stimuli such as pro-inflammatory cytokines, bacterial lipopolysaccharides, and viral RNA and DNA, via the activation of membrane and cytosolic receptors. (jcmtjournal.com)
  • tumour
  • Bypass of platinum-induced DNA adducts during DNA replication by specialised DNA polymerases may contribute to drug tolerance and tumour cell resistance. (nuigalway.ie)
  • metabolism
  • 2 ] DSB can be induced by environmental exposure such as ultraviolet (UV) or ionizing radiation (IR), as well as by endogenous agents like reactive oxygen species generated by cell metabolism. (jcmtjournal.com)
  • signaling cascade
  • This study particularly focuses on DNA damage-induced NF-κB signaling cascade and its physiological and pathological significance in B cell development and cancer therapeutic resistance. (jcmtjournal.com)
  • cisplatin
  • In a panel of oral/laryngeal squamous cell carcinoma (SCC) cell lines, we observed deficiencies in DNA damage signaling in correlation with cisplatin resistance, but not with DNA repair. (aacrjournals.org)
  • Wip1 knockdown or inhibition enhanced DNA damage signaling and resensitized oral SCC cells to cisplatin. (aacrjournals.org)
  • Following treatment with cisplatin or carboplatin, nascent DNA strands were on average up to 39% shorter in cells lacking pol eta than in cells expressing pol eta, consistent with a role for pol eta in bypass of platinum-induced lesions during DNA replication in vivo. (nuigalway.ie)
  • activation
  • Using network analysis, we found that differential activation of the SP1 and NUPR1 transcriptional regulators was predicted in directly irradiated and bystander H1299 cells. (springer.com)
  • Transcription factor prediction analysis also implied that HIF1α (Hypoxia induced factor 1 alpha) activation by protein stabilization in irradiated cells could be a negative predictor of the bystander response, suggesting that local hypoxic stress experienced by cells directly exposed to radiation may influence whether or not they will elicit a bystander response in neighboring cells. (springer.com)
  • Here, we quantitatively measure temporal and spatial changes of receptor occupancy, G-protein activation by FRET imaging, and PIP3 levels by monitoring the dynamics of PH(Crac)-GFP translocation in single living cells in response to different chemoattractant fields. (jove.com)
  • embryonic stem
  • We demonstrated that Mrad9 null enhances chromatid aberration frequency induced by radiation in bystander mouse embryonic stem cells. (springer.com)
  • Molecular Biol
  • Quantitative Imaging of Single Live Cells Reveals Spatiotemporal Dynamics of Multistep Signaling Events of Chemoattractant Gradient Sensing in Dictyostelium Molecular Biology of the Cell. (jove.com)
  • yeast
  • In both human and yeast cells, the middle subunit of RPA (RPA2) is phosphorylated during the S and G 2 phases of the cell cycle ( 10 ). (pnas.org)
  • regulate
  • Several cell surface receptors, their cognate ligands and downstream effectors regulate Golgi organization, suggesting a large regulatory network. (embopress.org)
  • G-protein-coupled receptors (GPCR) are known to regulate virtually all aspects of cell biology and comprise the single largest family of targets for current pharmaceuticals (reviewed in ref. 1 ). (aacrjournals.org)
  • control
  • Positive Control: Hela cell lysate. (acris-antibodies.com)
  • Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum Xuehua Xu 1 , Tian Jin 1 1 Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health Here, we describe detailed live cell imaging methods for investigating chemotaxis. (jove.com)
  • acquired resistance
  • The authors also discuss promising strategies for selectively targeting this genotoxic NF-κB signaling aiming to antagonize acquired resistance and resensitize refractory cancer cells to cytotoxic treatments. (jcmtjournal.com)
  • Biology
  • Trends in Cell Biology 19(1): 29-41. (els.net)
  • In your first year, your modules give you an insight into various biological and chemical disciplines, including biochemistry, cell and molecular biology, microbiology and physiology. (kent.ac.uk)