• To identify the domains essential for virion incorporation and nuclear localization, site-directed mutations were introduced into the vpx gene of SIVsmPBj1.9 and functionally analyzed. (duke.edu)
  • and (iv) a mutation at E30 (predicted to disrupt an N-terminal alpha-helix) had no effect on either virion incorporation or nuclear localization of Vpx. (duke.edu)
  • Importantly, mutations in Vpx which impaired nuclear localization also reduced virus replication in macaque macrophages, suggesting an important role of the carboxyl terminus of Vpx in nuclear translocation of the viral preintegration complex. (duke.edu)
  • Taken together, these results indicate that virion incorporation and nuclear localization are encoded by two partially overlapping domains in the C-terminus of Vpx (aa 60 to 112). (duke.edu)
  • Also, this region may contain nuclear localization signals and protein-protein interaction sites. (indigobiosciences.com)
  • Scholars@Duke publication: Control of cyclin B1 localization through regulated binding of the nuclear export factor CRM1. (duke.edu)
  • We show here that the previously characterized cytoplasmic retention sequence of Cyclin B1, responsible for its interphase cytoplasmic localization, is actually an autonomous nuclear export sequence, capable of directing nuclear export of a heterologous protein, and able to bind specifically to the recently identified export mediator, CRM1. (duke.edu)
  • We propose that the observed cytoplasmic localization of Cyclin B1 during interphase reflects the equilibrium between ongoing nuclear import and rapid CRM1-mediated export. (duke.edu)
  • They all contain a conserved Rel homologous domain (RHD) consisting of 300 amino acids at the N-terminus, which has multiple functions including DNA binding, homologous or heterologous dimerization, nuclear localization, and interaction with members of the IkB family. (xcessbio.com)
  • By using fluorescent fusion proteins, we show that TAF10 does not have an intrinsic nuclear localization signal (NLS) and needs one of its three interaction partners to be transported into the nucleus. (hal.science)
  • Thus, here we describe a novel role of the three mammalian interacting partners in the nuclear localization of TAF10, and our data suggest that a complex network of regulated cytoplasmic associations may exist among these factors and that this network is important for the composition of different TFIID and TFTC-type complexes in the nucleus. (hal.science)
  • Our studies showed that homologous and heterologous interactions of the N and P proteins are required for formation of the subnuclear viroplasms, and that nuclear localization of the N and P proteins requires interactions with different nuclear import proteins. (berkeley.edu)
  • A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
  • Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins. (bvsalud.org)
  • A comprehensive database of coregulators for nuclear receptors and other transcription factors was previously maintained at the Nuclear Receptor Signaling Atlas website which has since been replaced by the Signaling Pathways Project website. (wikipedia.org)
  • The ability of nuclear receptors to alternate between activation and repression in response to specific molecular cues, is now known to be attributable in large part to a diverse group of cellular factors, collectively termed coregulators and including coactivators and corepressors. (wikipedia.org)
  • The study of nuclear receptors owed a debt to decades of historical endocrinology and pathology, and prior to their discovery there was a wealth of empirical evidence that suggested their existence. (wikipedia.org)
  • As far back as the early 1970s, receptor-associated nonhistone proteins were known to support the function of nuclear receptors. (wikipedia.org)
  • A biochemical strategy designed in Myles Brown's laboratory provided the first direct evidence of ligand-dependent recruitment by nuclear receptors of ancillary molecules. (wikipedia.org)
  • Early cell transfection experiments had shown that discrete regions of certain receptors, such as thyroid hormone receptor, were sufficient to repress, or silence, reporter genes when fused to DNA-binding domains of heterologous transcription factors, suggesting that specific cellular factors - or corepressors - might bind to these regions and silence receptors in cells. (wikipedia.org)
  • Again, using the yeast two-hybrid screen, two corepressors were isolated in rapid succession, nuclear receptor corepressor, or NCoR, in Geoff Rosenfeld's laboratory, and silencing mediator of retinoid and thyroid receptors, or SMRT, by Ron Evans. (wikipedia.org)
  • Understand Nuclear Receptors through our introduction to this family of intracellular transcription factors that regulate gene expression. (indigobiosciences.com)
  • Unlike receptors found on the cell surface, members of the nuclear hormone receptor (NR) superfamily are restricted to metazoan organisms such as nematodes, insects, and vertebrates. (indigobiosciences.com)
  • Chronically high insulin levels may disrupt the close apposition of β-adrenergic receptors and PKA, identifying a new mechanism for crosstalk between heterologous signal transduction pathways. (nature.com)
  • Therefore, Sig1R demonstrates properties that can be attributed to both chaperone proteins and receptors. (frontiersin.org)
  • Several of the SNF and SWI genes of Saccharomyces cerevisiae encode proteins that are involved in the regulation of transcriptional activation. (bdbiosciences.com)
  • The retinoblastoma protein, Rb, stimulates the transcription of a number of genes. (bdbiosciences.com)
  • TFIID, comprising the TATA box binding protein (TBP) and 13 TBP-associated factors (TAFs), plays a role in nucleation in the assembly of the RNA polymerase II preinitiation complexes on protein-encoding genes. (hal.science)
  • Our goal was to develop a general and convenient strategy to improve heterologous protein synthesis in Pichia pastoris without mutating the expressed genes. (biomedcentral.com)
  • Here, a large-scale deletion library of ribosomal protein (RP) genes was constructed for heterologous protein expression in Pichia pastoris , and 59% (16/27) RP deletants have significantly increased heterologous protein yield. (biomedcentral.com)
  • This is due to the delay of 60S subunit assembly by deleting non-essential ribosomal protein genes or 60S subunit processing factors, thus globally decreased the translation elongation speed and improved the co-translational folding, without perturbing the relative transcription level and translation initiation. (biomedcentral.com)
  • Maternal genes involved in dorsoventral (D/V) patterning of the Drosophila embryo interact to establish a stable nuclear concentration gradient of the Dorsal protein which acts as the morphogen along this axis. (silverchair.com)
  • In eukaryotic cells the genetic material is surrounded by a membrane system called the nuclear envelope (NE). (brookes.ac.uk)
  • Mid-SUN proteins are a neglected family of conserved type III membrane proteins of ancient origin with representatives in plants, animals and fungi. (brookes.ac.uk)
  • The role of SUN3 in the ER is reinforced by the identification of a protein interaction between SUN3 and the ER membrane-bound transcription factor maMYB. (brookes.ac.uk)
  • The results highlight the importance of mid-SUNs as functional components of the ER and outer nuclear membrane. (brookes.ac.uk)
  • N-linked glycosylation is the most prevalent posttranslational modification of plasma membrane and secretory proteins and participates in many important biological roles such as protein folding, intracellular targeting, immune response, cell adhesion, and protease resistance. (jneurosci.org)
  • I further investigated membrane proteins and the targeting of tail-anchored proteins at Sheffield Hallam University. (brookes.ac.uk)
  • I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
  • The yeast two-hybrid protein-protein interaction assay led to the identification of an array of receptor-interacting factors in David Moore's laboratory and RIP140 repressive protein was discovered in Malcolm Parker's laboratory. (wikipedia.org)
  • The recombinant NcACBP protein was expressed to verify the binding activities of NcACBP in vitro , and the heterologous expression of NcACBP in Δacbp yeast in vivo . (biomedcentral.com)
  • A yeast complementation assay showed that heterologous expression of NcACBP rescued the phenotypic defects in Δacbp yeast, indicating of the binding activity of NcACBP in vivo . (biomedcentral.com)
  • Plant and yeast cells were used to characterize this complex by investigating the interactions of the virus and host proteins required for establishment of the subnuclear replication site. (berkeley.edu)
  • Our studies have demonstrated that several of the proteins encoded by cellular oncogenes function in fundamental aspects of gene regulation. (stanford.edu)
  • The vpx gene products of human immunodeficiency virus type 2 (HIV-2) and of the closely related simian immunodeficiency viruses from sooty mangabeys (SIVsm) and macaques (SIVmac) comprise a 112-amino-acid virion-associated protein that is critical for efficient virus replication in nondividing cells such as macrophages. (duke.edu)
  • These proteins are intracellular transcription factors that directly regulate gene expression in response to lipophilic molecules. (indigobiosciences.com)
  • The lilli gene encodes a nuclear protein related to the AF4/FMR2 family. (sdbonline.org)
  • The discovery of inactivating mutations in the BRCA1-associated protein 1 gene in sporadic and hereditary mesothelioma has opened up a variety of novel molecular, clinical, and diagnostic investigations. (nih.gov)
  • Deletion analysis of two types of chloramphenicol acetyltransferase reporter gene constructs under control of either the Ucp promoter or a heterologous herpes simplex virus-tk promoter defined an enhancer in a 220-bp HindIII-XbaI fragment which was essential for both brown fat specificity and norepinephrine inducibility. (elsevierpure.com)
  • This protein belongs to the rel proto-oncogene and NF-KB transcriptional factor family and acts by controlling zygotic gene expression. (silverchair.com)
  • Genotyping of ASFV isolates by partial sequencing of the B646L gene that encodes the major capsid protein p72 has identified 22 genotypes ( 6 ). (cdc.gov)
  • We observed a distinction in the repeat lengths of the circumsporozoite protein (CSP) gene sequences between P. brasilianum and P. malariae. (cdc.gov)
  • We are studying the effects and consequences of protein fusion on the transcriptional and transforming activities of these proteins using in vitro and animal models. (stanford.edu)
  • In vitro analysis revealed that a putative switch-protein kinase regulator, RsbW, is capable of interacting directly with σ 66 , as well as phosphorylating its own antagonist, RsbV1, rendering it inactive. (plos.org)
  • It codes for a 'b-HLH' DNA-bind-ing protein which can dimerize and bind to DNA in vitro and to polytene chromosomes in vivo. (silverchair.com)
  • The NcACBP recombinant protein was able to specifically bind acyl-CoA esters in vitro . (biomedcentral.com)
  • When the NLS sequences of either TAF8 or SPT7L are mutated, TAF10 remains cytoplasmic, but a heterologous NLS can drive TAF10 into the nucleus. (hal.science)
  • Experiments using fluorescence recovery after photobleaching show that TAF10 does not associate with any cytoplasmic partner but that once transported into the nucleus it binds to nuclear structures. (hal.science)
  • The cytoplasmic-nuclear transport of TAF10 is naturally observed during the differentiation of adult male germ cells. (hal.science)
  • Plant Dicer-like proteins (DCLs) are RNase III, double-stranded RNA (dsRNA)-specific endonucleases with specialized functions in producing short (s)RNAs of 21- to 24-nucleotides (nt), including micro (mi)RNAs and short interfering (si)RNAs of endogenous or viral origin. (biomedcentral.com)
  • I took up a position at Oxford Brookes University in 2012 investigating the role of reticulon proteins in ER tubulation and viral trafficking in order to develop my international reputation in ER research and advanced imaging. (brookes.ac.uk)
  • Three of the common proteins (designated N, P, and L) form the nucleocapsid core, which has an RNA-dependent-RNA-polymerase (RdRp) activity that functions in transcription of mRNAs and replication of the viral genomic and antigenomic RNAs. (berkeley.edu)
  • Virus particles also contain a matrix protein that coils the nucleocapsid and a surface glycoprotein that spans the viral envelope to associate with the matrix protein and the nucleocapsid core. (berkeley.edu)
  • The animal rhabdoviruses replicate in the cytoplasm and the best studied example, vesicular stomatitis virus (VSV), encodes five proteins. (berkeley.edu)
  • We measured this using an improved fluorescent reporter and by phosphorylation of endogenous cAMP-response-element binding protein (CREB). (nature.com)
  • Egan, J. J., Greenberg, A. S., Chang, M. K. & Londos, C. Control of endogenous phosphorylation of the major cAMP-dependent protein kinase substrate in adipocytes by insulin and beta-adrenergic stimulation. (nature.com)
  • Presumably, this relocalization promotes the phosphorylation of nuclear targets critical for chromatin condensation and nuclear envelope breakdown. (duke.edu)
  • thus, we propose that Cyclin B1 phosphorylation at the G2/M transition prevents its interaction with CRM1, thereby reducing nuclear export and facilitating nuclear accumulation. (duke.edu)
  • The nuclear import of TAF10 is regulated by one of its three histone fold domain-containing interaction partners. (hal.science)
  • The nuclear transcription factor NF-κB is a dimer composed of Rel protein family members. (xcessbio.com)
  • We are investigating how Pbx and Meis proteins contribute to the specificity of Hox function in development and how disruption of their activities leads to neoplasia. (stanford.edu)
  • Hormones mobilize intracellular second messengers and initiate signalling cascades involving protein kinases and phosphatases, which are often spatially compartmentalized by anchoring proteins to increase signalling specificity 1 . (nature.com)
  • Opt for the final set of presynaptic proteins similar to that seen for cla-1 mutants, might be a conserved mechanism to alter granule function coincides with enlargement of granules by overexpression may be sufficient to activate decapping, but would not confer substrate specificity, leading to a clean kamagra online without prescription 1. (sophieluis.com)
  • Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. (bdbiosciences.com)
  • Decelerating translation elongation speed has been shown to improve the soluble protein yield when expressing heterologous proteins in industrial expression hosts. (biomedcentral.com)
  • Coregulators are often incorrectly referred to as cofactors, which are small, non-protein molecules required by an enzyme for full activity, e.g. (wikipedia.org)
  • In the early 1990s, some investigators such as Keith Yamamoto had suggested a role for non-DNA nuclear acceptor molecules. (wikipedia.org)
  • The molecules that mediate the sorting of synaptic kamagra online without prescription vesicle proteins, we simultaneously examined synaptic vesicle. (sophieluis.com)
  • Nuclear magnetic resonance (NMR)-based metabolomic studies of human tumors have identified a marked increase in the intracellular concentration of phosphocholine relative to normal tissues. (nih.gov)
  • Analyzing this domain in greater detail, we identified a 26-amino-acid (aa 60 to 85) fragment that was sufficient to mediate the transport of a heterologous protein (green fluorescent protein [GFP]) to the nucleus. (duke.edu)
  • The discovering that a restricted peptide series formulated with the IL-1α NLS is totally enough to mediate energetic nuclear transport of heterologous proteins indicates that additional non-NLS regions of the molecule are not required for the establishment of N-terminal IL-1α nuclear transport. (bioerc-iend.org)
  • Neuronal microtubule-associated protein 2D is a dual A-kinase anchoring protein expressed in rat ovarian granulosa cells. (nature.com)
  • Feliciello, A., Rubin, C. S., Avvedimento, E. V. & Gottesman, M. E. Expression of a kinase anchor protein 121 is regulated by hormones in thyroid and testicular germ cells. (nature.com)
  • PKA: a portrait of protein kinase dynamics. (nature.com)
  • This study provides evidence that a switch-protein kinase regulatory network controls availability of σ 66 , the main sigma subunit for transcription in Chlamydia . (plos.org)
  • Long-term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells. (jci.org)
  • To examine the role of protein kinase C as a chronic regulator of proximal tubule Na/H antiporter activity, the effect of phorbol 12-myristate 13-acetate (PMA) on the Na/H antiporter was studied in cultured proximal tubule cells. (jci.org)
  • Short-term activation of protein kinase C by 5 min exposure to PMA caused an acute increase in Na/H antiporter activity that was not prevented by cycloheximide or actinomycin D and did not persist 24 h later. (jci.org)
  • Long-term activation of protein kinase C by 2 h exposure to PMA caused a dose-dependent increase in Na/H antiporter activity 24 h later. (jci.org)
  • This latter effect was due to protein kinase C activation in that it was inhibited by sphingosine and was not seen with 4 alpha-PMA, an inactive analogue. (jci.org)
  • In conclusion, short-term activation of protein kinase C leads to a transient increase in Na/H antiporter activity that is independent of transcription and translation, whereas long-term activation of protein kinase C causes a persistent increase in antiporter activity that is dependent on transcription and translation and is associated with increased mRNANa/H abundance. (jci.org)
  • Phosphatidic acid is generated from the cleavage of phosphatidylcholine by phospholipase D2 and is a key activator of the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/AKT survival signaling pathways. (nih.gov)
  • For example, green fluorescent protein (GFP) is a eukaryotic protein containing 11 beta-sheet structures and its folding yield was significantly increased by co-translational folding in E. coli [ 14 ]. (biomedcentral.com)
  • Lipid synthesis requires bulk carbon skeleton acyl-CoAs, the transport of which depends on the acyl-CoA binding protein (ACBP). (biomedcentral.com)
  • Before entering lipid synthesis pathways, different kinds of acyl-CoA transporters which acts as the major carrier of acyl-CoAs, such as fatty acid binding protein (FABP), sterol carrier protein 2 (SCP2) and acyl-CoA-binding protein (ACBP), would activate and convert fatty acids to fatty acyl-CoA esters via a reaction catalyzed by fatty acyl-CoA synthetase and transported to various metabolic locations [ 11 ]. (biomedcentral.com)
  • The evolutionary history of MyoD1 and MyoD2 is compared to the presence of this polyserine region using phylogenetics, and its functional relevance is tested using overexpression in a heterologous system to investigate subcellular localisation, stability, and activity of MyoD proteins that include and do not include the polyserine region. (mdpi.com)
  • Here I pursued my scientific interests in subcellular protein localisation, bioinformatics, and mathematical modelling. (brookes.ac.uk)
  • My additional independent research on ER localisation and splicing in auxin biosynthesis showed for the first time ER-localisation for an auxin biosynthetic protein. (brookes.ac.uk)
  • The translational pausing sites, which are mediated by clustered but non-consecutive slow-translating codons, coordinate protein biosynthesis and co-translational folding [ 6 ]. (biomedcentral.com)
  • This includes finding out what other proteins the SUNs bind to and what functions they have during cell division. (brookes.ac.uk)
  • Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)
  • In support of this hypothesis, we found that treatment of cells with leptomycin B, which disrupted Cyclin B1-CRM1 interactions, led to a marked nuclear accumulation of Cyclin B1. (duke.edu)
  • 1) Characterize novel families of oncoproteins (Pbx and Meis) that dimerize with and regulate the DNA binding properties of Hox proteins. (stanford.edu)
  • Nuclear receptor coregulators are a class of transcription coregulators that have been shown to be involved in any aspect of signaling by any member of the nuclear receptor superfamily. (wikipedia.org)
  • Finally, ligation of the COX-2 3'-UTR to a heterologous reporter failed to confer dexamethasone sensitivity. (mdc-berlin.de)
  • Tbis motif was also found to be able to direct a heterologous protein, the Gag protein of human immunodeficiency virus, into the nucleus. (uni-wuerzburg.de)
  • The D-domain appears to allow for conformational changes in the protein structure following ligand binding. (indigobiosciences.com)
  • These results suggest that the experience from the NLS is certainly latent in the unchanged precursor which proteolytic processing leads to the introduction of nuclear localizing activity presumably because of a conformational transformation. (bioerc-iend.org)
  • The binding of allosteric modulators to a target protein induces a conformational change in the protein structure and changes the activity of orthosteric ligands ( Figure 1 ). (frontiersin.org)
  • By using the vaccinia virus expression system, a conserved basic sequence motif in the nucleocapsid domain of foamy virus Cag All foamy viruses give rise to a strong nuclear staining when infected cells are reacted with sera from infected hosts. (uni-wuerzburg.de)
  • By using the vaccinia virus expression system, a conserved basic sequence motif in the nucleocapsid domain of foamy virus Cag proteins was identified to be responsible for the nuclear transport of the gag precursor molecule. (uni-wuerzburg.de)
  • A potential expression platform for efficient pharmaceutical proteins and industrial enzymes production was provided without synonymous mutation. (biomedcentral.com)
  • Pichia pastoris is a widely used platform for heterologous protein expression, which is a "generally regarded as safe" (GRAS) microorganism. (biomedcentral.com)
  • Unlike the bacterial expression systems, which lack the modification enzymes, Pichia pastoris is able to produce heterologous proteins with post-translational modifications, especially glycosylation, which is crucial for optimal properties of many pharmaceutical proteins. (biomedcentral.com)
  • Malcolm Parker's laboratory was the first to show that a recurring structural feature of many coactivators is an alpha-helical LXXLL motif (a contiguous sequence of 5 amino acids where L = leucine and X = any amino acid), or nuclear receptor box, present from a single to several copies in many coactivators, which is implicated in their ligand-dependent recruitment by the receptor AF-2. (wikipedia.org)
  • Recent studies have revealed that the amino acid sequence does not guarantee correct folding of proteins. (biomedcentral.com)
  • This was demonstrated by the nearly identical genomic organization and multiple sequence alignments for the merozoite surface proteins msp3 and msp7. (cdc.gov)
  • Several mutations in the N terminus of the G-protein-coupled receptor rhodopsin disrupt NXS/T consensus sequences for N-linked glycosylation (located at N2 and N15) and cause sector retinitis pigmentosa in which the inferior retina preferentially degenerates. (jneurosci.org)
  • Mutations affecting glycosylation of the heptahelical G-protein-coupled receptor rhodopsin are associated with retinitis pigmentosa (RP), a disease characterized by progressive degeneration of photoreceptors. (jneurosci.org)
  • It should be noted that others have reported around the nuclear translocation of the 17-kDa. (bioerc-iend.org)
  • We report our analysis of MyoD1 and MyoD2 protein sequences from 54 teleost species, and show that O. alcalica , along with some other teleosts, include a polyserine repeat between the amino terminal transactivation domains (TAD) and the cysteine-histidine rich region (H/C) in MyoD1. (mdpi.com)
  • However, due to the remarkable codon preference and tRNA content against the other species, heterologous proteins expressed in Pichia pastoris often encounter folding problem, which severely limited their production efficiency [ 3 ]. (biomedcentral.com)
  • These are frequently activated by fusion to other transcriptional proteins resulting in chimeric transcription factors. (stanford.edu)
  • We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)
  • The human Brm protein has been reported to be a 180 kDa nuclear factor that acts as a transcriptional activator when fused to a heterologus DNA binding domain. (bdbiosciences.com)
  • Singh P, Coe J, Hong W. A role for retinoblastoma protein in potentiating transcriptional activation by the glucocorticoid receptor. (bdbiosciences.com)
  • Repression of IL-1beta-induced PGE2 release, COX activity, and COX-2 protein by actinomycin D was only effective within the first hour following IL-1beta treatment, while dexamethasone was effective when added up to 10 h later, suggesting a functional role for post-transcriptional mechanisms of repression. (mdc-berlin.de)
  • Recruitment of corepressors, generally occurring in the absence of ligand, depends on a critical conformation of the receptor AF-2 domain, as well as upon nuclear receptor box-like helical motifs in the corepressor. (wikipedia.org)
  • Competitive DNA mobility shift assays with probes of the CRE and BRE motifs indicate that nuclear proteins interact with these motifs in a cooperative, synergistic manner. (elsevierpure.com)
  • The first authentic, common nuclear receptor coactivator was steroid receptor coactivator 1, or SRC-1 (NCOA1), first cloned in Bert O'Malley's laboratory. (wikipedia.org)
  • Crystal structure of the N-terminal domain of the effector protein SidI of Legionella pneumophila reveals a glucosyl transferase domain. (resist-cluster.de)
  • Allosteric regulation is the regulation of protein activity by binding an effector molecule at a site other than the orthosteric or active site of a protein ( Figure 1 ). (frontiersin.org)
  • Tli1tox-, a cognate immunity protein, directly interacts with Tle1Vc to neutralize its toxicity. (bvsalud.org)
  • CycB signal that did not observe mutants arising in multiple PAM-distal mismatches on phage immunity provided by Cas12a, we developed a heterologous kamagra online without prescription type V-A CRISPR-Cas12a system in Escherichia coli. (sophieluis.com)
  • Global decrease in the translation elongation speed by RP deletion enhanced co-translational folding efficiency of nascent chains and decreased protein aggregates to improve heterologous protein yield. (biomedcentral.com)
  • The identification of a novel 26-amino-acid nuclear targeting domain provides a new tool to investigate the nuclear import of the HIV-2/SIV preintegration complex. (duke.edu)
  • Scholars@Duke publication: Functional analysis of the simian immunodeficiency virus Vpx protein: identification of packaging determinants and a novel nuclear targeting domain. (duke.edu)
  • We have identified a cytosolic acyl-CoA binding protein, with highly conserved amino acid residues and a typical acyl-CoA binding domain in N. caninum . (biomedcentral.com)
  • All foamy viruses give rise to a strong nuclear staining when infected cells are reacted with sera from infected hosts. (uni-wuerzburg.de)
  • Cells had been chosen with 800 μg/ml G418 one cell-subcloned and screened for IL-1α proteins appearance using dot-blot evaluation of cytosolic ingredients. (bioerc-iend.org)
  • Traditional western blot analyses of control Tarafenacin and IL-1α33-transfected MC cytosolic and nuclear ingredients had been performed. (bioerc-iend.org)
  • Nuclear pellets had been retrieved by centrifugation at 2000 × oocyte cytosolic fractions that permit nuclear transportation of NLS-containing protein (22). (bioerc-iend.org)
  • lymphocyte cytosolic protein 2 (SH2 domain. (wikigenes.org)
  • The non-denatured recombinant CP fusion protein was purified using the NiCNTA agarose method (Qiagen, MD, USA) as explained previously (Liu BL21 (DE3) harboring pET-32a induced with and without 0.5?mmol/L IPTG. (thetechnoant.info)
  • Results of the assays indicated the four MAbs reacted strongly and specifically with approximately 30?kDa WDV CP in the WDV-infected wheat samples as well as the 50?kDa recombinant WDV CP fusion protein (Fig.?2). (thetechnoant.info)
  • Lane 3, purified recombinant Rabbit Polyclonal to SSTR1 WDV CP fusion protein. (thetechnoant.info)
  • This Frontiers Research Topic aims to highlight recent advances made to examine the role of the nuclear envelope (NE) as the "key border" in plants. (brookes.ac.uk)
  • During my PhD studies I identified two such proteins - the Sad1/Unc84 (SUN) domain proteins. (brookes.ac.uk)
  • Another protein, phytase (Phy) from Citrobacter amalonaticus CGMCC 1696, has 95% homology to E. coli -derived phytase which consists of one α-domain containing five α-helices and a β-hairpin, and one α/β-domain including seven β-sheets and four α-helices [ 15 ]. (biomedcentral.com)
  • These proteins are beta-sheet rich or multi-domain and aggregation prone, which may require higher co-translational folding efficiency. (biomedcentral.com)
  • My research focuses on studying protein components of the plant NE. (brookes.ac.uk)
  • I'm using cell and molecular biology techniques, biochemistry as well as microscopy to characterise the plant SUN proteins. (brookes.ac.uk)
  • I published the first report of plant ER reticulon protein interactors by Co-IP and FRET-FLIM. (brookes.ac.uk)
  • Lane 1, protein from a healthy wheat plant. (thetechnoant.info)
  • Lane 2, protein from a WDV-infected wheat plant. (thetechnoant.info)
  • Translational regulation played an important role in the correct folding of heterologous proteins to form bioactive conformations during biogenesis. (biomedcentral.com)