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  • odorant
  • Our understanding of mammalian olfactory coding has been impeded by the paucity of information about the odorant receptors (ORs) that respond to a given odorant ligand in awake, freely behaving animals. (jneurosci.org)
  • Because each olfactory sensory neuron expresses a single OR gene, multiple ORs responding to a given odorant ligand can be identified simultaneously by capturing the population of activated olfactory sensory neurons and using expression profiling methods to screen the repertoire of mouse OR genes. (jneurosci.org)
  • This novel assay, called the Kentucky in vivo odorant ligand-receptor assay, should facilitate the in vivo identification of mouse ORs for a given odorant ligand of interest. (jneurosci.org)
  • Here, we demonstrate the ability of a novel in vivo assay in mice, supported by heterologous expression data, to identify ORs that respond to a given odorant ligand. (jneurosci.org)
  • Much current work on shape theory focuses on neural processing, rather than the specific interaction between odorant and receptor that generates the original signal. (wikipedia.org)
  • on the one side are a majority of sensory scientists who argue that our odorant receptors detect specific scent molecules on the basis of their shapes and chemical properties. (wikipedia.org)
  • Olfactory receptors (ORs), also known as odorant receptors, are expressed in the cell membranes of olfactory receptor neurons and are responsible for the detection of odorants (i.e., compounds that have an odor) which give rise to the sense of smell. (wikipedia.org)
  • An important difference, though, is that the odorant has to be able to become resident in the receptor for a response to be generated. (wikipedia.org)
  • However, once the link between receptor response and duration of the residence of the odorant in the receptor is recognised, differences in odor between enantiomers can be understood: molecules with different handedness may spend different amounts of time in a given receptor, and so initiate responses of different intensities. (wikipedia.org)
  • Any binding interaction of an odorant molecule with an olfactory receptor will therefore be likely to show some isotope effect upon deuteration, and the observation of an isotope effect in no way argues exclusively for a vibrational theory of olfaction. (wikipedia.org)
  • Synaptic
  • Data locating neurosteroid binding sites on synaptic αβγ GABA A receptors are sparse and inconsistent. (asahq.org)
  • Alphaxalone binds in transmembrane β + -α − pockets of synaptic GABA A receptors that are adjacent and intracellular to sites for the potent anesthetics etomidate and propofol. (asahq.org)
  • subunits
  • Expression of the α9 and α10 subunits is largely restricted to the hair cells of the inner ear (Elgoyhen et al. (aspetjournals.org)
  • Receptors of this family are arranged as five subunits surrounding a central pore, with each subunit composed of four α helical transmembrane segments. (wikipedia.org)
  • The adult form of the GlyR is the heteromeric α1β receptor, which is believed to have a stoichiometry (proportion) of three α1 subunits and two β subunits or four α1 subunits and one β subunit. (wikipedia.org)
  • The α-subunits are also able to form functional homo-pentameric receptors in heterologous expression systems in African clawed frog's oocytes or mammalian cell lines, and the α1 homomeric receptor is essential for studies of channel pharmacokinetics and pharmacodynamics. (wikipedia.org)
  • nAChRs
  • Although functional recombinant nAChRs generated by the coexpression of α9 and α10 in Xenopus laevis oocytes have been described previously, there have been no reports of the successful heterologous expression of α9α10 nAChRs in cultured cell lines. (aspetjournals.org)
  • The pharmacological profile of α9χα10χ receptors, determined by equilibrium radioligand binding studies, is broadly similar to that determined previously by electrophysiological studies conducted with native and recombinant α9α10 nAChRs. (aspetjournals.org)
  • Despite successful studies in oocytes, there have been no reports of the heterologous expression of α9- or α10-containing nAChRs, as either homomeric or heteromeric complexes, in other expression systems. (aspetjournals.org)
  • odor receptors
  • Three years after Moncrieff proposed the theory, John Amoore speculated further that the over ten thousand smells distinguishable by the human olfaction system resulted from the combination of seven basic primary odors correlating to odor receptors for each, much as the spectrum of perceived colors in visible light is generated by the activation of three primary color receptors. (wikipedia.org)
  • Sperm cells also express odor receptors, which are thought to be involved in chemotaxis to find the egg cell. (wikipedia.org)
  • Novel
  • Arthropod hormone receptors are potential targets for novel pesticides as they regulate many essential physiological and behavioral processes. (jove.com)
  • Relying on RET, Javitch and coworkers showed that RET signals were more characteristic of random proximity between receptors, rather than an actual bond formation between receptors, suggesting that discrepancies in binding profiles may be the result of downstream interactions, rather than novel effects due to oligomerization. (wikipedia.org)
  • molecules
  • To explain these phenomena, we analyze the stochastic model of the local motions of molecules around the receptor. (jove.com)
  • takes aim at the vibrational theory of olfaction, finding no evidence that olfactory receptors distinguish vibrational states of molecules. (wikipedia.org)
  • amino
  • The receptor can be activated by a range of simple amino acids including glycine, β-alanine and taurine, and can be selectively blocked by the high-affinity competitive antagonist strychnine. (wikipedia.org)
  • bind
  • The expression of NF-κB, the transcription factor that regulates inflammatory processes, is promoted by the constitutive activity of the H1 receptor as well as by agonists that bind at the receptor. (wikipedia.org)
  • mammalian cell
  • The kinin receptors from the southern cattle tick, Boophilus microplus (Canestrini), and the mosquito Aedes aegypti (Linnaeus), were stably expressed in the mammalian cell line CHO-K1. (jove.com)
  • intracellular
  • Functional analyses of these receptors were completed using a calcium bioluminescence plate assay that measures intracellular bioluminescence to determine cytoplasmic calcium levels upon peptide application to these recombinant cells. (jove.com)
  • As the kinin receptor signals through the release of intracellular calcium, the intensity of the signal is related to the potency of the peptide. (jove.com)