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CCAAT-Enhancer-Binding Protein-alphaCCAAT-Enhancer-Binding ProteinsCCAAT-Enhancer-Binding Protein-betaCCAAT-Enhancer-Binding Protein-deltaEnhancer Elements, GeneticPromoter Regions, GeneticDNA-Binding ProteinsTranscription FactorsNuclear ProteinsTranscription Factor CHOPBase SequenceGene Expression RegulationMolecular Sequence DataAdipocytesTranscription, GeneticCell DifferentiationAdipogenesisBinding SitesRNA, MessengerTranscriptional ActivationCell LineElectrophoretic Mobility Shift AssayProtein Binding3T3-L1 CellsResponse ElementsTransfectionCells, CulturedGranulocytesCell Nucleus3T3 CellsGene ExpressionPPAR gammaMyelopoiesisLiverDown-RegulationLeucine ZippersChromatin ImmunoprecipitationDNAGenes, ReporterTrans-ActivatorsSignal TransductionDimerizationMutationTumor Cells, CulturedBlotting, WesternLuciferasesAdipose TissueEndrinDNA PrimersRegulatory Sequences, Nucleic AcidUp-RegulationGene Expression Regulation, EnzymologicNF-kappa B p50 SubunitMice, KnockoutHepatocyte Nuclear Factor 3-betaProtein IsoformsHeLa CellsAcute-Phase ReactionAmino Acid SequenceCyclic AMP Response Element-Binding ProteinRNA Polymerase Sigma 54Reverse Transcriptase Polymerase Chain ReactionMice, TransgenicCarrier ProteinsNF-kappa BReceptors, Cytoplasmic and NuclearDexamethasoneU937 CellsCell Line, TumorMice, Inbred C57BLTranscription Factor AP-2Recombinant Fusion ProteinsPhosphorylationOligonucleotidesBlotting, NorthernTranscription Factor AP-1GlucocorticoidsMutagenesis, Site-DirectedGene Expression Regulation, LeukemicLeukemia, Myeloid, AcuteRepressor ProteinsRNA, Small InterferingEpithelial CellsEndoplasmic Reticulum StressGene Expression Regulation, NeoplasticHL-60 CellsHepatocytesInterleukin-6Monocyte-Macrophage Precursor CellsImmunohistochemistryProto-Oncogene ProteinsEndoplasmic ReticulumLipopolysaccharidesApoptosisPlasmidsCell ProliferationCarcinoma, HepatocellularMonosaccharide Transport ProteinsGene Expression ProfilingCell Division
PeptidesHomologous proteinProto-OncogeneTranscription factorsMRNAsIsoformsInhibitorHomodimersMotifsTranscriptionalRelAGenesApoptosisLeucineDominant-negativeSynthesisPeptideELISA KitHomologySubunitsFormTransactivationDDIT3GeneEndoplasmic reticulumSequenceAutophagyAmino acidInteractEnzymeWestern blotFamilyDimerizationDetectionAssayPlaysCellsFunctionsExpressionNormalCellRoleRegion
Peptides3
  • Unlike miRNAs whose central function is to restrain mRNA translation by inducing degradation, lncRNAs can act as numerous roles to exert their functions by directly or indirectly interacting with DNA, RNA or protein, even can encode some short peptides (Fig. 3 a-d) [ 6 ]. (biomedcentral.com)
  • Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. (affbiotech.com)
  • The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS . (lookformedical.com)
Homologous protein1
  • CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner (1). (affbiotech.com)
Proto-Oncogene3
  • Proto-Oncogene Proteins c-rel" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)
  • This graph shows the total number of publications written about "Proto-Oncogene Proteins c-rel" by people in this website by year, and whether "Proto-Oncogene Proteins c-rel" was a major or minor topic of these publications. (childrensmercy.org)
  • Below are the most recent publications written about "Proto-Oncogene Proteins c-rel" by people in Profiles. (childrensmercy.org)
Transcription factors3
MRNAs4
  • The eIF2α kinases phosphorylate Ser51 of eIF2α which leads to suppression of global protein synthesis but selective enhancement of translation of some mRNAs, such as that encoding ATF4. (biomedcentral.com)
  • Cellular identity is determined by the transcriptional profile which comprises the subset of mRNAs, and therefore proteins, being expressed by a cell at a given point in time. (silverchair.com)
  • A substantial portion of the regulatory interactions in the higher eukaryotic cell are mediated by simple sequence motifs in the regulatory segments of genes and (pre-)mRNAs, and in the intrinsically disordered regions of proteins. (biomedcentral.com)
  • Finally, we contrast the regulatory properties of protein motifs and the regulatory elements of DNA and (pre-)mRNAs, advocating that co-regulation, co-operativity, and motif-driven regulatory programs are common mechanisms that emerge from the use of simple, evolutionarily plastic regulatory modules. (biomedcentral.com)
Isoforms1
  • Multiple protein isoforms, including truncated, dominant negatives, are generated by translation of the C/EBPβ transcript or via proteolytic cleavage of the full-length C/EBPβ protein. (johnshopkins.edu)
Inhibitor2
  • The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. (novusbio.com)
  • Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. (affbiotech.com)
Homodimers1
  • It functions in homodimers and also heterodimers with C/EBP-β (beta) and C/EBP-γ (gamma), regulate the expression of genes that involves in different cellular responses, such as mitotic growth arrest and differentiation, cell cycle regulation, metabolism, osteoporosis, and in immunity, as well as in body weight homeostasis. (gentarget.com)
Motifs2
  • We observe that each gene and its products have a unique set of DNA, RNA or protein motifs that encode a regulatory program to define the logical circuitry that guides the life cycle of these biomolecules, from transcription to degradation. (biomedcentral.com)
  • DNA-binding motifs formed from two alpha-helixes which intertwine for about eight turns into a coiled coil and then bifurcate to form Y shaped structures. (lookformedical.com)
Transcriptional2
  • Furthermore the transcription elements CCAAT/enhancer-binding proteins α (C/EBPα) and STAT3 had been found to be engaged in the essential positive transcriptional legislation of ITGB6 in OSCC cells. (researchatlanta.org)
  • p100 and p105 can however undergo limited proteolysis to generate p52 and p50, respectively, which can form heterodimers with Rel proteins to form transcriptional activators [ 5 ]. (oncotarget.com)
RelA2
Genes4
  • The encoded protein is important in the regulation of genes involved in immune and inflammatory responses, and may be involved in the regulation of genes associated with activation and/or differentiation of macrophages. (wikipedia.org)
  • The C/EBP-α (CCAAT/enhancer-binding protein, alpha), is a family of transcription factor and recognizes the CGCAAT motif in the promoters of target genes. (gentarget.com)
  • Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. (affbiotech.com)
  • Cellular DNA-binding proteins encoded by the rel gene (GENES, REL). (childrensmercy.org)
Apoptosis2
Leucine1
  • A transfer RNA which is specific for carrying leucine to sites on the ribosomes in preparation for protein synthesis. (lookformedical.com)
Dominant-negative2
  • Gene deletion of individual C/EBP family members has demonstrated an essential role for C/EBPβ in normal mammary development, while transgenic and overexpression studies provide evidence that the dominant-negative C/EBPβ-liver-enriched inhibitory protein isoform induces proliferation in mammary epithelial cells. (johnshopkins.edu)
  • Further, the GR gene expresses a splicing variant GRbeta, which does not bind glucocorticoids but functions as a dominant negative isoform for GRalpha. (brainimmune.com)
Synthesis4
  • Phosphorylation of eIF2α on Ser51 inhibits 5' cap-dependent mRNA translation, resulting in the global suppression of protein synthesis to facilitate adaptation to a variety of stresses linked to protein synthesis, including proteotoxic stress, viral replication, heme depletion and amino acid withdrawal [ 2 ]. (biomedcentral.com)
  • GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. (affbiotech.com)
  • The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4). (affbiotech.com)
  • AMPK pathways inhibit biosynthetic pathways with energy consumption, such as protein, fatty acid and glycogen synthesis. (springer.com)
Peptide1
  • Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. (affbiotech.com)
ELISA Kit2
  • Description: A sandwich ELISA kit for detection of Mdm2 p53 Binding Protein Homolog from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (1elisakits.com)
  • Description: A sandwich ELISA kit for detection of Baculoviral IAP Repeat Containing Protein 6 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (1elisakits.com)
Homology1
  • This family of proteins comprises two subfamilies that share a DNA-binding and dimerization domain called the Rel homology domain (RHD) [ 4 ] and form homo- or hetero- dimers. (oncotarget.com)
Subunits4
  • Introduction CGS 21680 HCl Integrins are heterodimeric trans-membrane proteins that are composed of non-covalently associated and subunits [1]. (researchatlanta.org)
  • To date 18 and 8 subunits have been identified and are capable of forming more than 24 heterodimers that account for the structural and functional diversity of the integrin family [2]. (researchatlanta.org)
  • Twenty alpha-amino acids are the subunits which are polymerized to form proteins . (lookformedical.com)
  • Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. (lookformedical.com)
Form3
  • It can also form heterodimers with the related protein CEBP-alpha. (wikipedia.org)
  • The mutated proteins can interact with normal proteins to form dysfunctional tetramers, and thus change the pattern of genome methylation ( 5 ). (frontiersin.org)
  • Binds DNA as a dimer and can form stable heterodimers with C/EBP delta. (lu.se)
Transactivation1
  • The second subfamily of proteins (p105 and p100) has a C-terminal region that contains multiple copies of ankyrin repeats, instead of a transactivation domain, and can bind to and inhibit Rel proteins. (oncotarget.com)
DDIT31
  • Figure 1: Western blot analysis using DDIT3 mAb against human DDIT3 (AA: 87-192) recombinant protein. (promab.com)
Gene6
  • CCAAT/enhancer-binding protein delta is a protein that in humans is encoded by the CEBPD gene. (wikipedia.org)
  • The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. (wikipedia.org)
  • Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. (novusbio.com)
  • It also allows to generate your own reporting cell line in your desired cell type for study or screen of pathway specific gene-knockdown, over-expression, or chemical / drug/protein treatment in the cell based assay. (gentarget.com)
  • The integrin β6 (ITGB6) gene which encodes the limiting subunit of the integrin αvβ6 heterodimer plays CGS 21680 HCl a significant role in wound healing and carcinogenesis. (researchatlanta.org)
  • The human GR protein is produced from 9 exons of the GR gene and is composed of 3 major subdomains, each of which has specific structural and functional properties. (brainimmune.com)
Endoplasmic reticulum1
  • Three arms exist in the ER stress response: the protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1 (IRE1) pathways, where each of the sensor proteins is a membrane protein ( Figure 1 ). (encyclopedia.pub)
Sequence2
  • When C/EBP binds to C/EBP-TRE sequence, the downstream reporter is expressed as the result of activation for the minimal promoter. (gentarget.com)
  • The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. (lookformedical.com)
Autophagy1
  • During ER stress-a state of functional imbalance-adaptive and restorative programs such as the unfolded protein response (UPR) and ER-associated protein degradation (ERAD), or autophagy, come into play [ 12 ] [ 13 ] . (encyclopedia.pub)
Amino acid2
Interact1
Enzyme3
  • The mammalian ER stress response consists of three arms: the inositol-requiring enzyme 1 (IRE1), protein kinase R (PKR)-like ER kinase (PERK), and activating transcription factor 6 (ATF6) pathways. (encyclopedia.pub)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Mdm2 p53 Binding Protein Homolog (MDM2) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (1elisakits.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Mdm2 p53 Binding Protein Homolog (MDM2) in tissue homogenates, cell lysates and other biological fluids. (1elisakits.com)
Western blot1
  • WB: For western blot detection of denatured protein samples. (affbiotech.com)
Family1
  • IκBζ, an atypical member of the nuclear IκB family of proteins, is expressed at low levels in most resting cells, but is induced upon stimulation of Toll-like/IL-1 receptors through an IRAK1/IRAK4/NFκB-dependent pathway. (oncotarget.com)
Dimerization1
  • In the PERK arm, during stress, PERK dissociates from the chaperone protein, binding immunoglobulin protein/glucose-regulated protein 78 (BiP/GRP78), and undergoes dimerization and phosphorylation. (encyclopedia.pub)
Detection1
  • Description: A sandwich quantitative ELISA assay kit for detection of Mouse Mdm2 p53 Binding Protein Homolog (MDM2) in samples from tissue homogenates, cell lysates or other biological fluids. (1elisakits.com)
Assay1
  • Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes. (novusbio.com)
Plays1
  • The Golgi apparatus not only plays key roles in the transport, processing, and targeting of proteins, but also functions as a sensor of stress, signaling through the Golgi stress response. (encyclopedia.pub)
Cells1
Functions1
  • Long noncoding RNAs (lncRNAs) are a class of endogenous, non-protein coding RNAs that are highly linked to various cellular functions and pathological process. (biomedcentral.com)
Expression1
  • αvβ6 expression depends on integrin β6 (ITGB6) expression as ITGB6 only partners with αv forming a single heterodimer. (researchatlanta.org)
Normal1
  • Zahnow, CA 2002, ' CCAAT/enhancer binding proteins in normal mammary development and breast cancer ', Breast Cancer Research , vol. 4, no. 3, pp. 113-121. (johnshopkins.edu)
Cell2
  • Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). (novusbio.com)
  • Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. (novusbio.com)
Role1
  • Mounting evidence suggests that alterations in the ratio of the C/EBPβ-liver-enriched inhibitory protein isoform and the C/EBPβ-liver-enriched activating protein isoform may play a role in the development of breast cancer. (johnshopkins.edu)
Region1
  • The DNA-binding residues are located in the bifurcated region of the Y. (lookformedical.com)