AntibodiesSecondary Antibody ConjugatesConjugationMaleimideBiotinEsterCompoundsNucleic acidsLinkerPolyethylene glycolSulfhydrylCysteineHydrogelsPurificationFluorescentTherapeuticsAmineSpacerSurfacesAffinityChapterMolecularHighDetectionPreparationMoleculesLinearCharacterizationProductsReactionGroupsCellConcentrationVarietyDrugIntroductionTechnologyResearchSmallGroupPotentialTimeCellsStorageEffect
Antibodies11
- Among them AAT Bioquest's Buccutite™ crosslinking technology offer the most efficient method to conjugate antibodies with macromolecules such as enzyme tags. (stratech.co.uk)
- In addition, this 18 F-FBEM protocol can also be applied to label thiol-containing proteins, antibodies, as well as 5'-thio-functionalized oligonucleotides. (researchsquare.com)
- Cysteine engineered anti-MUC16 antibodies (Ab) are conjugated with one or more drug moieties (D) through a linker (L) to form cysteine engineered anti-MUC16 antibody-drug conjugates having Formula I: Ab-(L-D)p I where p is 1 to 4. (justia.com)
- The cysteine engineered antibodies may be conjugated with chemotherapeutic drugs, toxins, affinity ligands such as biotin, and detection labels such as fluorophores. (justia.com)
- The invention also relates to methods of using antibodies and antibody-drug conjugate compounds for in vitro, in situ, and in vivo diagnosis or treatment of mammalian cells, or associated pathological conditions. (justia.com)
- Some cytotoxic drugs tend to be inactive or less active when conjugated to large antibodies or protein receptor ligands. (justia.com)
- Whether it's Biotinylated Antibodies, Antibody-Enzyme Conjugates, or Fluorescently-Labeled Antibodies, our technology creates secondary antibody reagents with unmatched performance and stability. (quantabiodesign.com)
- Antibodies biotinylated with our biotinylation reagents retain stability and will not aggregate or precipitate in aqueous solution. (quantabiodesign.com)
- When our biotinylated antibodies and proteins are combined with our streptavidin conjugates made with our SuperHydrophilic crosslinkers, they provide performance never before possible using traditional hydrophobic reagents and conjugates. (quantabiodesign.com)
- Our current product, Biotinylated Protein A ( AQ-70021 ), can be used to detect and probe rabbit and human antibodies (with a preference for IgG isotypes) in a range of immunoassay applications. (quantabiodesign.com)
- G-Biosciences Immobilized Monomeric Avidin Resin is designed for the simple affinity chromatography purifications of proteins, antibodies and other molecules with a biotin tag. (gbiosciences.com)
Secondary Antibody Conjugates2
- AQ Secondary Antibody conjugates are made using our unique dPEG ® technology, which results in unmatched SuperHydrophilic reagents. (quantabiodesign.com)
- There are no other sources of SuperHydrophilic secondary antibody conjugates with the properties of AQ Advanced Antibody Technology . (quantabiodesign.com)
Conjugation5
- Dojindo's labeling kits contain centrifugal Filtration Tubes that are used for protein purification, conjugation reaction, condensation, and buffer exchange. (dojindo.com)
- 1. Protein Conjugation (Amine PEGylation) to PEG Hydrogel - Bioartificial matrices for therapeutic vascularization, Proc Natl Acad Sci USA. (creativepegworks.com)
- 5. PEG-PCL Hydrogel and Conjugation of Proteins - Shape Memory RGD-Containing Networks: Synthesis, Characterization, and Application in Cell Culture, Macromol. (creativepegworks.com)
- 1. Polyethylene Glycol PEG Linkers in Antibody Drug Conjugation Antibody-drug conjugates (ADCs) targeting antibody delivers a covalently attached payload to a cell type of interest, wherein the molecule is internalized and lysosomally processed and the payload is released. (axispharm.com)
- The AQuora dyes are available with a variety of reactive groups for conjugation to proteins, peptides, and nucleic acids. (quantabiodesign.com)
Maleimide6
- Most protein labeling reagents have similar reactive sites, such as succinimidyl ester (NHS) for amino groups and maleimide or bromoacetamide for sulfhydryl groups. (dojindo.com)
- Bismaleimides are a class of compounds with two maleimide groups connected by the nitrogen atoms via a linker, and are used as crosslinking reagents in thermoset polymer chemistry. (wikipedia.org)
- Compounds containing a maleimide group linked with another reactive group, such as an activated N-hydroxysuccinimide ester, are called maleimide heterobifunctional reagents (for example, see SMCC reagent). (wikipedia.org)
- Due to exceptionally fast reaction rates and significantly high selectivity towards cysteine residues in proteins, a large variety of maleimide heterobifunctional reagents are used for the preparation of targeted therapeutics, assemblies for studying proteins in their biological context, protein-based microarrays, or proteins immobilisation. (wikipedia.org)
- For instance, emerging promising targeted drug therapies, antibody-drug conjugates, are constituted of three main components: a monoclonal antibody, a cytotoxic drug, and a linker molecule often containing a maleimide group, which binds the drug and the antibody. (wikipedia.org)
- MAL-PEG-NH2 has a maleimide and an amino group at each end of the molecular chain and is a linear heterofunctional PEG reagent. (bocsci.com)
Biotin8
- Of these, fluorescent, biotin, and enzyme labeling reagents are used most frequently for antibody labeling. (dojindo.com)
- These kits can be used to prepare conjugates with enzyme, fluorophore, and biotin (Table 1). (dojindo.com)
- We are revolutionizing the Streptavidin/Biotin field by completely redesigning biotinylation reagents and streptavidin conjugates using our novel SuperHydrophilic dPEG ® technology. (quantabiodesign.com)
- Biotin reagents made with SuperHydrophilic linkers eliminate this problem. (quantabiodesign.com)
- An assortment of reagents for the purification of biotin labelled proteins are available from G-Biosciences. (gbiosciences.com)
- Accurate estimation of the molar ratio of biotin conjugated to protein/antibody is easy and affordable with HOOK™ BiotinQuant™ Kit . (gbiosciences.com)
- HOOK™ Cell Surface Protein Isolation Kit uses G-Biosciences HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. (gbiosciences.com)
- HOOK™ BiotinQuant™ uses HABA (2-(4-Hydroxyphenylazo)benzoic acid/ 2-(4′-Hydroxybenzeneazo)benzoic acid/ 4′-Hydroxyazobenzene-2-carboxylic acid) to rapidly estimate the mole-to-mole ratio of biotin to antibody or protein. (gbiosciences.com)
Ester2
- Acrylate-PEG-SCM is a linear heterobifunctional PEG reagent with an Acrylate and an activated NHS ester. (creativepegworks.com)
- JenKem Technology's 8arm PEG Norbornene (tripentaerythritol) derivatives with hydrolytically degradable ester linkage can be crosslinked into PEG hydrogels. (jenkemusa.com)
Compounds3
- Sometimes, protein labeling with NH 2 -reactive compounds is not favorable because of the potential blockage of the protein's active site. (dojindo.com)
- The sugar chain of a protein is also a possible labeling site with hydrazide compounds. (dojindo.com)
- Traditional biotinylation compounds are hydrophobic and cause antibody or protein precipitation. (quantabiodesign.com)
Nucleic acids1
- iFluor™ superior fluorescent labelling dyes, optimised for labelling proteins and nucleic acids. (stratech.co.uk)
Linker1
- APN-C3-PEG4-alkyne is a heterobifunctional linker containing an APN moiety with exquisite chemoselectivity for cysteine and an alkyne group. (broadpharm.com)
Polyethylene glycol3
- Maleimides linked to polyethylene glycol chains are often used as flexible linking molecules to attach proteins to surfaces. (wikipedia.org)
- 3. Crosslinking Proteins to PEG Hydrogels - Photocrosslinkable laminin-functionalized polyethylene glycol hydrogel for intervertebral disc regeneration, Acta Biomaterialia 10 (2014) 1102-1111, Â Text . (creativepegworks.com)
- 2. Polyethylene Glycol PEG Linkers in PEGylation PEGylation of a molecule is the process by which PEG is either non-covalently or covalently attached to a potential protein, peptide, or antibody of interest to produce alterations in the physiochemical properties of the macromolecule in areas like conformation, electrostatic binding and hydrophobicity. (axispharm.com)
Sulfhydryl1
- In this way, MAL-PEG-NH2 can be used to modify proteins, peptides, or other surfaces containing sulfhydryl groups. (bocsci.com)
Cysteine1
- The superior stability of APN-cysteine conjugates in aqueous media, human plasma, and living cells makes this new thiol-click reaction a promising methodology for applications in bioconjugation. (broadpharm.com)
Hydrogels2
- 2. Crosslinking Proteins to PEG Hydrogels - Injectable laminin-functionalized hydrogel for nucleus pulposus regeneration, Biomaterials, 2013 (34): 7381-7388, Â Text . (creativepegworks.com)
- Wilson, RL, et al, Protein-functionalized poly (ethylene glycol) hydrogels as scaffolds for monolayer organoid culture. (jenkemusa.com)
Purification1
- Labeling requires 1) purification of protein and buffer exchange, 2) labeling reaction, and 3) purification and recovery of the conjugate. (dojindo.com)
Fluorescent1
- In addition, our new Fluorescent-Labeled Streptavidin reagents are made with our SuperHydrophilic AQuora Dyes, providing brighter signals with a lower background than when using traditional dyes. (quantabiodesign.com)
Therapeutics1
- For biomedical applications, we are combining rational design and combinatorial methods to create designer protein therapeutics and diagnostic agents. (stanford.edu)
Amine1
- Acrylate-PEG-SCM may be used to introduce an amine reactive functional group in crosslinked PEG hydrogel. (creativepegworks.com)
Spacer1
- It is a useful crosslinking reagent with a PEG spacer. (creativepegworks.com)
Surfaces1
- a sensitive and robust tool for detection of proteins in solutions and solid surfaces (such as gels and membranes). (stratech.co.uk)
Affinity1
- Our Affinity Proteins product area will be growing soon to include all the major immunoglobulin binding proteins as well as a selection of carbohydrate-binding proteins (lectins). (quantabiodesign.com)
Chapter1
- See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products. (quantabiodesign.com)
Molecular1
- at the same time developing protein and peptide-based tools that will allow us to manipulate cellular processes on a molecular level. (stanford.edu)
High5
- Overall, a conjugate prepared by NH 2 labeling is adequate for general assays while a conjugate prepared by SH labeling can be used for high-sensitivity assays. (dojindo.com)
- Because these three processes can be performed in a single Filtration Tube, recovery of conjugated proteins is always high, nearly 100% of protein can be recovered from the tube, and reproducibility is high as well. (dojindo.com)
- Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters. (stratech.co.uk)
- Dr. Cochran's translational interests span protein-based drug discovery and development for applications in oncology and regenerative medicine, and the development of new technologies for high-throughput protein analysis and engineering. (stanford.edu)
- When matched with the appropriate Antibody-HRP or Streptavidin-HRP conjugate made with our unique SuperHydrophilic technology, the result is extremely high sensitivity and low background in immunoassays and western blots. (quantabiodesign.com)
Detection1
- The combined benefits of SuperHydrophilic streptavidin or antibody conjugates along with ultra-sensitive detection with our Q-Bright Chemiluminescent Substrates will provide superior performance over any other similar commercial products. (quantabiodesign.com)
Preparation1
- 10. Preparation of Protein-Polymer Conjugates: Copolymerisation by RAFT, 2020, DOI: 10.1071/CH19514. (creativepegworks.com)
Molecules2
- Attaching the other end of the polyethylene chain to a bead or solid support allows for easy separation of protein from other molecules in solution, provided these molecules do not also possess thiol groups. (wikipedia.org)
- Drug moieties used in antibody drug conjugates include bacterial protein toxins such as diphtheria toxin, plant protein toxins such as ricin, small molecules such as auristatins, geldanamycin (Mandler et al (2000) J. of the Nat. (justia.com)
Linear1
- It is a linear heterobifunctional PEG reagent, which is a lipophilic lipid PEG conjugate with good water solubility. (bocsci.com)
Characterization1
- Synthesis and Characterization of a New class of Cationic Protein Polymers for Multivalent Display and Biomaterial Applications. (quantabiodesign.com)
Products1
- All of these products will be labeled and conjugated using our unique SuperHydrophilic technology to provide enhanced performance not available elsewhere. (quantabiodesign.com)
Reaction1
- Protein labeling reagents are used for various assays that are coupled with antigen-antibody reaction. (dojindo.com)
Groups1
- Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. (creativepegworks.com)
Cell2
- Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells. (dojindo.com)
- In the current study, we assessed the cytotoxic effects of idarubicin-Z HER2 affibody conjugate on the positive-HER2 cancer cell lines. (iranpath.org)
Concentration2
- The average concentration of protein will be 500-1,000 μg per ml as antibody. (dojindo.com)
- The constructed Idarubicin-Z HER2 affibody conjugate decreased the viability of SK-OV-3 cells at its optimal concentration, more efficiently and specifically than other treated cells. (iranpath.org)
Variety1
- Dojindo offers a wide variety of protein labeling kits. (dojindo.com)
Drug1
- The use of antibody-drug conjugates (ADC), i.e. immunoconjugates, for the local delivery of cytotoxic or cytostatic agents, i.e. drugs to kill or inhibit tumor cells in the treatment of cancer (Lambert, J. (2005) Curr. (justia.com)
Introduction1
- BACKGROUND: The incidence of invasive meningococcal disease due to serogroup C (MenC) decreased in Portugal since the introduction of the conjugate vaccine (MCC) in the free market in 2001 and in the National Immunisation Plan in 2006. (bvsalud.org)
Technology2
- Our Streptavidin-HRP conjugates are also linked through our SuperHydrophilic technology. (quantabiodesign.com)
- Protein A, isolated from Staphylococcus aureus, is biotinylated using our SuperHydrophilic technology, which is designed to improve solubility, limit non-specific binding, and improve signal-to-noise ratios. (quantabiodesign.com)
Research2
- Dojindo offers the following protein labeling reagents and kits for protein research and proteomics. (dojindo.com)
- Reagent grade, for research purpose. (broadpharm.com)
Small1
- Through this simple centrifuge process, small proteins can be removed, leaving only conjugated proteins to be recovered by pippetting with a buffer solution. (dojindo.com)
Group2
- Therefore, labeling at the NH 2 group on a protein is fairly reliable and simple method for preparing a conjugate. (dojindo.com)
- Most proteins do not have an SH group, just a disulfide group. (dojindo.com)
Potential1
- It appears the Z HER2 -affibody conjugate of idarubicin has great potential to be implicated as an innovative anti-cancer agent in future clinical trials in patients with HER2-overexpressing ovarian cancer. (iranpath.org)
Time2
- The cytotoxic effects of constructed idarubicin-Z HER2 affibody conjugate on the SK-BR-3, SK-OV-3, and MCF-7 cells with various levels of HER2 expression were evaluated by MTT assay after 48 hours of incubation time. (iranpath.org)
- CLS-PEG-MAL can be used for the manufacture of liposomes to shorten the cycle time of encapsulated drugs, as well as for non-viral transfection reagents. (bocsci.com)
Cells4
- The Z HER2 -affibody conjugate of idarubicin has a more specific cytotoxic effect compared with idarubicin alone against HER2-overexpressing ovarian cancerous cells. (iranpath.org)
- The Z HER2 -affibody conjugate of idarubicin has a more potent cytotoxic effect than idarubicin alone against HER2-overexpressing malignant cells. (iranpath.org)
- Long-term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells. (jci.org)
- To examine the role of protein kinase C as a chronic regulator of proximal tubule Na/H antiporter activity, the effect of phorbol 12-myristate 13-acetate (PMA) on the Na/H antiporter was studied in cultured proximal tubule cells. (jci.org)
Storage2
- Additionally, the kit's unique buffer system prevents aggregation of protein and conjugate during labeling and storage of the conjugate. (dojindo.com)
- The conjugate is then recovered with storage buffer or WS buffer. (dojindo.com)
Effect1
- This latter effect was due to protein kinase C activation in that it was inhibited by sphingosine and was not seen with 4 alpha-PMA, an inactive analogue. (jci.org)