• Antibody (or DNA testing) for HHV-8 is insufficiently sensitive/specific to predict risk of Kaposi sarcoma. (nih.gov)
  • Effective suppression of HIV replication with ART is recommended to reduce the risk of HHV-8-associated Kaposi sarcoma (strong, low) . (nih.gov)
  • Effective suppression of HIV replication with ART is recommended for all patients with evidence of active Kaposi sarcoma and other HHV-8-associated malignant lymphoproliferative disorders (strong, very low) . (nih.gov)
  • Life-long ART is recommended for all individuals with evidence of active or treated Kaposi sarcoma or other HHV-8-associated malignant lymphoproliferative disorders (strong, low) . (nih.gov)
  • Is there an indication for serologic testing for human herpesvirus 8 (HHV-8) in asymptomatic HIV-infected children (compared with not testing) to guide clinical management? (nih.gov)
  • Therefore, routine testing to identify HHV-8-seropositive, HIV-infected patients is not recommended (strong, very low) . (nih.gov)
  • Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies. (lsbio.com)
  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. (lsbio.com)
  • Alternatively, a variety of serological tests, including immunofluorescence assays ( 23 ), Western blotting ( 26 ), and enzyme-linked immunosorbent assays (ELISAs) ( 11 , 15 , 18 ), have been employed to detect antibodies to HHV-8 proteins and to diagnose infection. (asm.org)
  • Antibodies against either HHV-8 lytic antigen or HHV-8 latency-associated nuclear antigen (LANA) were detected, by immunofluorescence assays, in 339 Amerindians and 181 non-Amerindians from the Brazilian Amazon. (www.gov.uk)
  • Both blood and effusion samples were analyzed for lymphocyte and monocyte counts and/or HHV-8 antibodies and DNA. (biomedcentral.com)
  • TB patients with or without effusions had significantly greater HHV-8 seropositivity ( p = 0.009) and titers of HHV-8 antibodies ( p = 0.005) than healthy controls. (biomedcentral.com)
  • The seropositivity and blood titers of HHV-8 antibodies were similar in TB patients with and without effusions. (biomedcentral.com)
  • However, the presence of HHV-8 antibodies and DNA in the plasma and effusions of TB patients with pleural or peritoneal effusion has not yet been determined. (biomedcentral.com)
  • In particular, we assessed the positivity and titers of HHV-8 antibodies and the positivity and levels of HHV-8 DNA in blood and effusion samples of TB patients with pleural or peritoneal effusions. (biomedcentral.com)
  • Thus, 379 pregnant women attending ante-natal consultation in Saint Camille Medical Centre were subject to HIV, HHV-8 antibodies and the viral marker Hepatitis B Surface Antigen (HBsAg) detection. (scialert.net)
  • Of these APC, only B cells support complete, lytic HHV-8 infection. (frontiersin.org)
  • After induction of lytic replication of HHV-8 in BCP-1 cells with n-butyrate, we observed a major reduction in the expression of an ORF 73-specific 6.6-kb mRNA, indicating that this region is under latent control. (nih.gov)
  • the presence of vIRF-1 antisense RNA reduced the expression of several HHV-8 lytic genes, including interleukin-6 (IL-6) in TPA-treated BCBL-1 cells ( 26 ). (asm.org)
  • Quantitative detection of HHV-8 was performed by real-time PCR, and virus expression was evaluated by immunohistochemistry for latent and lytic HHV-8 antigens. (biomedcentral.com)
  • Increasing amounts of HHV-8 DNA were detected in placental tissues and culture supernatants and immunohistochemistry analyses demonstrated that both cyto- and syncitiotrophoblasts, as well as placental endothelial cells, expressed latent (see Figure 1 ) and lytic antigens. (biomedcentral.com)
  • To summarize the seroprevalence of human herpesvirus 8 (HHV-8) in mainland China, we conducted a systematic review and meta-analysis based on available literature. (cdc.gov)
  • Worldwide seroprevalence of HHV-8 varies: generally low to moderate for populations in Western countries and Asia ( 1 - 4 ) but as high as 50% for the general population in sub-Saharan Africa and higher for HIV-positive populations ( 5 - 7 ). (cdc.gov)
  • HHV-8 had similar seroprevalence in TB patients with and without effusions. (biomedcentral.com)
  • Indeed, the primary tropism of B cells by these gamma herpesviruses is uncommon among human virus infections. (frontiersin.org)
  • HHV-8 incident infection rates during this period were 3.2 and 5.3 infections/100 person-years for infants and mothers, respectively. (unl.edu)
  • Improved diagnostic reagents and testing are currently needed for the serological detection of human herpesvirus 8 (HHV-8) infections. (asm.org)
  • Healthy individuals with HHV-8 infections typically have no symptoms or signs of disease. (biomedcentral.com)
  • The aims of this research are: i) to evaluate the prevalence of HHV-8, HBV and HIV among pregnant women, ii) to determine the percentage of these co-infections and iii) to estimate the frequency of the mother-to-child transmission of HIV among HBV and HHV-8 positive mothers. (scialert.net)
  • Although we did not have a large sample which would show large prélalences of the infections, we could put forward that the Co-infection of the HIV with one of these viruses (HBV or HHV-8) could favorite the mother-to-child transmission. (scialert.net)
  • The exact mechanism by which HHV-8 infection leads to neoplastic disease has not been fully elucidated, but seroconversion to HHV-8 antibody positivity virtually always precedes development of the tumors. (nih.gov)
  • Most significant independent behavioral risk factors for HHV-8 seroconversion included increased risk with current breastfeeding (HR 2.1, P = 0.002), increased risk with use of pacifier (HR 9.0, P = 0.01), and greater risk with household members blowing on food prior to sharing with child (HR 2.8, P = 0.05). (unl.edu)
  • Immunohistochemical detection of the HHV-8 LANA protein in placental histocultures. (biomedcentral.com)
  • The presence of DNA of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2), varicella zoster virus (VZV), Epstein-Barr virus (EBV), human cytomegalovirus (HCMV), human herpesvirus 6 (HHV-6), human herpesvirus 7 (HHV-7) and human herpesvirus 8 (HHV-8) was searched by specific nested polymerase chain reaction. (springer.com)
  • Bello-Morales R, Fedetz M, Alcina A, Tabares E, Lopez-Guerrero JA (2005) High susceptibility of a human oligodendroglial cell line to herpes simplex type 1 infection. (springer.com)
  • Indirekter Immunofluoreszenztest (IFT) zum qualitativen und quantitativen Nachweis von anti-humanen Herpes-Virus 8 Antikör-pern aus Serum oder Plasma. (viramed.de)
  • We have recently cloned and characterized a second HHV-8 encoded vIRF, vIRF-2, that encodes a short protein of 163 amino acids (aa) ( 11 ). (asm.org)
  • Human Herpesvirus 8 Interleukin-6 Interacts with Calnexin Cycle Components and Promotes Protein Folding. (physiciansweekly.com)
  • In early KS lesions, VEGFR-3 is more extensively expressed than LNA-1, indicating that HHV-8 is not inducing the proliferation of VEGFR-3-positive endothelium directly. (pnas.org)
  • By various in situ hybridization techniques (including PCR in situ and RNA in situ hybridization), HHV-8 DNA is present in most of the tumor cells, so-called spindle cells, in KS lesions ( 3 - 8 ). (pnas.org)
  • Many of these gene products allow the virus to evade the human immune system ( 8 ). (asm.org)
  • In recent years, epidemiological and experimental studies have strengthened the link between this lymphotropic herpesvirus and KS by showing that HHV8 infection precedes development of tumors, tracks tightly with KS risk, and targets spindle cells ( 3 )( 4 )( 5 ). (rupress.org)
  • Ferrante P, Omodeo-Zorini E, Zuffolato MR, Mancuso R, Caldarelli-Stefano R, Puricelll S, Mediati M, Losciale I, Caput (1997) Human T-cell lymphotropic virus tax and Epstein-Barr virus DNA in peripheral blood of multiple sclerosis patients during acute attack. (springer.com)
  • Interstitial pneumonitis, combined with detection of HHV-8 in non HIV-infected patients, indicates a pathogenetic role of HHV-8 in unexplained lung diseases. (semanticscholar.org)
  • A) HHV-8-infected CRO-AP/3 cells showed a strongly positive nuclear immunostaining. (biomedcentral.com)
  • Âlvarez-Lafuente R, Martín-Estefanía C, De las Heras V, Castrillo C, Cour I, Picazo JJ, Varelade Seijas E, Arroyo R (2002) Prevalence of herpesvirus DNA in MS patients and healthy blood donors. (springer.com)
  • PCR analysis shows that HHV-8 is present in all epidemiologic types of KS (i.e. (pnas.org)
  • sexual and nonsexual transmission have been described ( 8 - 10 ). (cdc.gov)
  • Of these, 137 children seroconverted to become HHV-8 positive, an incidence of 29.94 per 100 child-years. (unl.edu)
  • Although a correlation between human herpesvirus 8 (HHV-8) infection and abortion or low birth weight in children has been reported [ 1 , 2 ] presently no information has been published regarding HHV-8 tropism for placenta. (biomedcentral.com)
  • Sexual transmission of HHV-8 might play an important role among high-risk group populations, such as gay and bi-sexual men in Western countries. (herpes.com)
  • These results suggest that LIPS screening using an antigen mixture is a sensitive and high-throughput method for serological screening of HHV-8 infection in individuals with KS. (asm.org)
  • However, the status of HHV-8 in the effusions of patients with TB remains unclear. (biomedcentral.com)
  • Plasma samples of 6 TB patients, but none of the healthy controls, were positive for HHV-8 DNA ( p = 0.03). (biomedcentral.com)
  • An HHV-8-specific cDNA clone was isolated. (nih.gov)
  • Among these, HHV-8 encodes four open reading frames (ORFs) that showed homology to the transcription factors of the interferon regulatory factor (IRF) family. (asm.org)
  • Currently, there is a need for sensitive and specific testing to identify HHV-8-infected individuals, especially among potential blood donors ( 14 ). (asm.org)
  • Human herpesvirus 8 (HHV-8) is one of the few human viruses that primarily targets these APC for infection, altering their cytokine profiles, manipulating their surface expression of MHC molecules, and altering their ability to activate HHV-8-specific T cells. (frontiersin.org)