• citation needed] Hep G2 cells and their derivatives are also used as a model system for studies of liver metabolism and toxicity of xenobiotics, the detection of environmental and dietary cytotoxic and genotoxic (and thus cytoprotective, anti-genotoxic, and cogenotoxic) agents, understanding hepatocarcinogenesis[citation needed], and for drug targeting studies[citation needed]. (wikipedia.org)
  • Increased incidence of chromosomal aberration were observed at cytotoxic concentrations of methyl isothiocynate in tests with mammalian cells, precluding any conclusion on its in vitro clastogenic potential. (europa.eu)
  • Toxicology in Vitro , 65 , Article 104797. (illinois.edu)
  • Wen, Y, Mirji, N & Irudayaraj, J 2020, ' Epigenetic toxicity of PFOA and GenX in HepG2 cells and their role in lipid metabolism ', Toxicology in Vitro , vol. 65, 104797. (illinois.edu)
  • The inhibitory effect of raloxifene, bazedoxifene, and acolbifene on LCA sulfonation was also observed in HepG2 human hepatocellular carcinoma cells. (aspetjournals.org)
  • NC exhibited rapid cellular uptake and stronger apoptotic effect (~46.6% reduced IC 50 value) than FC, blocking HepG2 cells at G2/M phase. (bgu.ac.il)
  • The results indicate that PAI-1 synthesis in presence of insulin is markedly increased in down-regulated cells, and that metformin inhibits this effect by acting at the cellular level. (jci.org)
  • With further technological developments, these human in vitro cellular models shall be highly useful in the screening of compounds for personalized medicine, allowing optimum therapy with minimum or no adverse effects, and in the study of adverse outcomes in different strata of population. (brill.com)
  • In this study, the antitumor effects of terpinen-4-ol and the cellular and molecular mechanisms responsible for it were evaluated and studied, respectively on human nonsmall cell lung cancer (NSCLC) cells. (hindawi.com)
  • Liver hepatocellular carcinoma cell line HepG2 was used as a model to study induced liver inflammation in vitro at the cellular, genetic, and epigenetic levels. (illinois.edu)
  • Another well-characterized polarized hepatocyte cell line is the rat hepatoma-derived hybrid cell line WIF-B. With the proper culture conditions, Hep G2 cells display robust morphological and functional differentiation with a controllable formation of apical and basolateral cell surface domains (van IJzendoorn et al. (wikipedia.org)
  • Plasminogen activator inhibitor-1 synthesis in the human hepatoma cell line Hep G2. (jci.org)
  • The hepatoma cell line Hep G2 has been shown to synthesize PAI-1 in response to insulin. (jci.org)
  • In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)
  • and effects on the cell cycle progression, action on the apoptosis induction, of leaf and stem extracts of R. rosaefolius, in HepG2/C3A cells (Human hepatoma). (fapesp.br)
  • The effect of Alcide®, a new antimicrobial drug, on rat blood glutathione and erythrocyte osmotic fragility, in vitro. (cdc.gov)
  • The Rubus rosaefolius plant recently have been some of its therapeutic properties confirmed by scientific analysis, among them the analgesic, antimicrobial, antihypertensive, antioxidant, antiproliferative effects in tumor cells, diuretic, gastroprotective and antidepressant. (fapesp.br)
  • Study evaluated crude ethanol and aqueous extracts of various parts of Senna spectabilis and S tora for in-vitro antimicrobial activity against E. coli, B. cereus, Candida albicans and S. cerevisiae. (stuartxchange.org)
  • The HepG2 cell line was transiently transfected with CYP2B6- and CYP3A4-luciferase promoter reporter plasmids along with human PXR (hPXR) or hCAR expression vectors. (cdc.gov)
  • 7] "Functional characterization of an organic cation transporter (hOCT1) in a transiently transfected human cell line (HeLa). (tcdb.org)
  • The aim of this study was to investigate the effects of Ishige sinicola , a brown alga, on osteoblast differentiation through the activation of the bone morphogenetic protein 2 (BMP-2)/runt-related transcription factor 2 (Runx2) signalling pathway in MC3T3-E1 cells. (degruyter.com)
  • A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining, and expression analysis of osteoblastic genes were carried out to assess MC3T3-E1 cell proliferation and osteoblastic differentiation. (degruyter.com)
  • In this study, the NTERA2-D1 (NT2) cells were induced towards differentiation (NT2-RA) with 10-5 M retinoic acid (RA) for three days and then subjected to various amounts of vitreous humor (VH). (bvsalud.org)
  • However, the use of bFGF for human ES cell culture, particularly at the high levels used by some for 'feeder-free' culture, must be considered carefully, as bFGF may drive ES cells towards differentiation. (ddw-online.com)
  • The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)
  • Hep G2 (or HepG2) is a human liver cancer cell line. (wikipedia.org)
  • This can be important for the study of human liver diseases that are caused by an incorrect subcellular distribution of cell surface proteins, e.g., hepatocanalicular transport defects such as Dubin-Johnson Syndrome and progressive familial intrahepatic cholestasis (PFIC), and familial hypercholesterolemia. (wikipedia.org)
  • In the current study, the antiproliferative activity of raw extracts and fractions of D. tertiolecta (clone CCMP 1320) was evaluated against four different human cancer cell lines: melanoma, hepatocellular liver carcinoma, and two lung adenocarcinoma cell lines. (frontiersin.org)
  • With the recent advances in human-cell cultivation techniques, allowing in vivo -like in vitro long-term functionality, there is a shift in focus towards the mechanistic details of the adverse effects "over time" aimed at a better understanding of the dynamics of biological processes. (brill.com)
  • In vitro methods, based on human primary cells, cell lines, and genetically modified reporter cell lines, have greatly expanded the scope of in vitro toxicology. (brill.com)
  • Komor, Badran and Liu, 2017 ) have opened up tremendous opportunities for the development of cell lines, especially those of human origin ( Tobita, Guzman-Lepe and de L'Hortet, 2015 ). (brill.com)
  • The anti‐proliferative activity of all newly synthesized compounds has been assessed against six human solid tumor cell lines. (researchgate.net)
  • All newly synthesized compounds were screened for anti‐proliferative activity against different human tumor cell lines. (researchgate.net)
  • Compound 5 showed high antiproliferative activity against several human tumor cell lines. (researchgate.net)
  • In-vitro studies are important, but they cannot be extrapolated to make any conclusion about what actually happens in a human body. (techarp.com)
  • Recent reports have indicated that terpinen-4-ol exerts its antitumor effects by triggering caspase-dependent apoptosis in human melanoma cells or by inducing necrotic cell death and cell-cycle arrest in mouse mesothelioma and melanoma cell lines without affecting normal cells [ 14 , 15 ]. (hindawi.com)
  • In addition, there is no report on the antitumor effects of terpinen-4-ol against human nonsmall cell lung cancer cells. (hindawi.com)
  • Therefore, in this study, the anticancer effects of terpinen-4-ol were evaluated on two NSCLC cell lines, namely, A549 and CL1-0 human lung adenocarcinoma cells. (hindawi.com)
  • The A549 human lung adenocarcinoma and CL1-0 lung adenocarcinoma cell lines were cultured in Dulbecco′s modified eagle medium supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic antimycotic. (hindawi.com)
  • Here, we generated a recombinant protein formed by linking ApoAI to the amino terminus of human PBGD (rhApoAI-PBGD) in an attempt to transfer PBGD into liver cells. (unav.edu)
  • Study of the cytotoxicity, genotoxicity, cell cycle monitoring, induction of apoptosis, and gene expression of Rubus rosaefolius leaves and stems extracts in human cultured cells. (fapesp.br)
  • Their cytocompatibility was assessed on cultured human and murine fibroblast cells and analyzed in relation to the adsorption of bovine serum albumin from the culture medium onto their surface. (infim.ro)
  • The tested nanocomposites showed excellent cytocompatibility to human fibroblast cells. (infim.ro)
  • Stem cells, and in particular human embryonic stem (ES) cells, have earned a reputation for being labour intensive and difficult to grow and control in culture. (ddw-online.com)
  • F urther complicating matters, human ES cells are typically co-cultured with feeder layers of mouse fibroblast cells. (ddw-online.com)
  • Several growth factors have been identified that promote growth of human ES cells in culture, most notably basic fibroblast growth factor (bFGF). (ddw-online.com)
  • Xeno-free media that do not rely upon high levels of bFGF have been developed that contain only humanised or synthetic components and are engineered to support the undifferentiated growth and expansion of human ES cells. (ddw-online.com)
  • Use of progenitor cell lines can eliminate the need to culture challenging human ES cells. (ddw-online.com)
  • For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)
  • More recently, additional human and animal cell lines that support SARS-CoV replication have been identified ( 15 ). (cdc.gov)
  • Furthermore, it is highly versatile and suitable for applications in the preclinical drug research and in vitro assays. (bvsalud.org)
  • For this, the first stage of the work will seek to observe effects of cytotoxicity of the extract, by MTT test, trypan blue staining, genotoxicity by comet and micronucleus assays, and on cell cycle progression and induction of apoptosis, by flow cytometry. (fapesp.br)
  • assays for evaluating induction are less well characterized and the relationships between in vitro and in vivo data less clear. (aspetjournals.org)
  • Our results indicated that terpinen-4-ol induced apoptosis through a mitochondria-mediated pathway in NSCLC cells and that the apoptosis elicited by terpinen-4-ol was p53 dependent. (hindawi.com)
  • Octyl gallate reduces ATP levels and Ki67 expression leading HepG2 cells to cell cycle arrest and mitochondria-mediated apoptosis. (harvard.edu)
  • Collectively, these data provide insight into the molecular mechanisms underlying terpinen-4-ol-induced apoptosis in NSCLC cells, rendering this compound a potential anticancer drug for NSCLC. (hindawi.com)
  • Here we characterize the metabolism of PCB 2 (3-chlorobiphenyl), an environmentally relevant LC-PCB congener, in HepG2 cells with in silico prediction and nontarget high-resolution mass spectrometry. (cdc.gov)
  • Since 2015, more than 3,000 articles were published on studies using crispr /Cas9 genome editing, including more than 900 articles using the technology in mammalian cells (PubMed, accessed June 11, 2017). (brill.com)
  • Measurement of lactase dehydrogenase (LDH) activity showed that nyaope exposure resulted in necrotic cell death in SH-SY5Y cells. (wits.ac.za)
  • A micronucleus assay in mice bone marrow cells was reported to be negative. (europa.eu)
  • For in vitro experiments HepG2 cells were used as surrogate liver cells and SH-SY5Y cells represented neurons. (wits.ac.za)
  • So soaking liver cells in a bak kut teh soup would most definitely not reflect what our liver cells actually experience after a bak kut teh meal. (techarp.com)
  • These drawbacks may be explained, at least in part, by the various phenotypes and histological characteristics of tumor liver cells and its microenvironment [ 5 ] . (encyclopedia.pub)
  • The purpose of the current studies was to characterize the effect of known inducers on CYP3A4 in Fa2N-4 cells, and to determine whether these in vitro data could reliably project the magnitude of DDIs caused by induction. (aspetjournals.org)
  • In Fa2N-4 cells, all 18 positive controls produced greater than 2-fold maximal CYP3A4 induction, and all 6 negative controls produced less than 1.5-fold maximal CYP3A4 induction. (aspetjournals.org)
  • There are several in vitro tools for assessment of CYP3A4 induction. (aspetjournals.org)
  • then, CYP3A4 levels are compared with those in vehicle-treated cells. (aspetjournals.org)
  • Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. (wikipedia.org)
  • In addition, a normal cell line (lung fibroblast) was used as toxicity control. (frontiersin.org)
  • Authors showed that a fraction from D. tertiolecta CCMP364 containing 95% of violaxanthin displayed 50% growth inhibition on the MCF7 breast cancer cell line at a concentration of 11.7 μg/mL. (frontiersin.org)
  • Seven compounds show good activity against single cell line whereas, compounds having a pyridine scaffold showed significant anti‐proliferative activity. (researchgate.net)
  • A dual cell-line GFP expression system was constructed to study EDs with activities as estrogen receptor agonists or antagonists. (cornell.edu)
  • The HepG2 cell line was used as an in vitro model for Wilson's disease. (sfu.ca)
  • By exploring the response of fresh primary duck embryonic fibroblasts to various cytotoxins and mycotoxins, we showed that primary DEF had a higher sensitivity to toxins compared to HepG2 cells, and thus great potential in serving as a cytotoxicity screening model in vitro. (purdue.edu)
  • The highest toxicity of saponins with IC50=0.22 mg/ml was observed in MDA-MB-123 cells. (irost.org)
  • On the other hand, the question remains whether in vitro response can be used to predict in vivo toxicity with sufficient accuracy. (purdue.edu)
  • Good in vitro estimation of the toxicity potential of sample may be useful in initial screening of feed and feed ingredients. (purdue.edu)
  • Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-fluorouracil (5-FU). (techscience.com)
  • Our study design included both in vitro and in vivo experiments. (wits.ac.za)
  • Results from the in vivo experiments reflect the in vitro findings with some evidence of the activation of apoptotic and autophagic pathways being observed, albeit differently in the two tissue types studied. (wits.ac.za)
  • The aim of this study was to analyze the insulin-mediated response of PAI-1 and lipid synthesis in Hep G2 cells after producing an insulin-resistant state by decreasing insulin receptor numbers. (jci.org)
  • Hep G2 cells may be a suitable model to study PAI-1 regulation in response to hyperinsulinemia. (jci.org)
  • This study examined the dose-dependency and receptor specificity of CYP induction in vitro and in vivo. (cdc.gov)
  • In this work, in vitro systems, including stable cells lines with estrogen induced green fluorescence protein (GFP) expression and micro cell culture analog (microCCA) devices, were developed to study EDs. (cornell.edu)
  • Other projects concern the study of lipid monolayers and bilayers as models of processes occurring at the surface of cell membranes, and the use of these monolayers in molecular recognition studies. (umsl.edu)
  • This study aimed to explore the effects of Acacia honey (AH) on corneal keratocytes morphology, proliferative capacity, cell cycle, gene and protein analyses. (blogspot.com)
  • In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
  • The study identified 10 new cell lines capable of supporting the replication of SARS-CoV and confirmed the susceptibility of 4 cell lines previously reported. (cdc.gov)
  • This study shows that SARS-CoV can be isolated in several cell lines commonly used for diagnostic or research purposes. (cdc.gov)
  • Morphology of cell lines was evaluated by invert microscopy. (irost.org)
  • These cells were treated with increasing concentrations of nyaope and the effects thereof on cell morphology, cell viability and cell death were subsequently investigated at various time points. (wits.ac.za)
  • These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice. (wikipedia.org)
  • Corneal keratocytes cultured in media supplemented with 0.025% AH showed an increase in proliferative capacity while retaining their morphology, gene and protein expressions with normal cell cycle. (blogspot.com)
  • Alternatively, stem cell cultures can be grown on extracellular matrix extracts and supplemented with conditioned medium from mouse fibroblast cultures. (ddw-online.com)
  • Myeloid immune cells, specifically tumor-associated macrophages (TAMs), may represent potential novel therapeutic targets in HCC, complementing current ablative or immune therapies. (cancerindex.org)
  • Increased sub-G1 population and annexin-V binding, activation of caspases 9 and 3, cleavage of poly(ADPribose) polymerase (PARP), and a decrease of mitochondrial membrane potential (MMP) indicated involvement of the mitochondrial apoptotic pathway in terpinen-4-ol-treated A549 and CL1-0 cells. (hindawi.com)
  • The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)
  • Given the potential for laboratory-associated severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infections, we must know which cell lines are susceptible to the virus. (cdc.gov)
  • Given the potential for SARS-CoV infection to occur in a laboratory setting, we must be aware of cell lines in which it can replicate. (cdc.gov)
  • To address this problem, we encapsulated chelidonine in biodegradable poly(lactide-co-glycolide) (PLGA) polymers and evaluated nano-chelidonine's (NCs) anti-cancer efficacy vis-à-vis free chelidonine (FC) against HepG2 cells and also evaluated its bioavailability in mice. (bgu.ac.il)
  • 0.92) were obtained, suggesting that Fa2N-4 cells can be used for identification of inducers as well as prediction of the magnitude of clinical DDIs. (aspetjournals.org)
  • SARS-CoV was first isolated in Vero E6 and FRhK cells injected with clinical specimens as part of early attempts to identify the etiologic agent of SARS ( 10 , 14 ). (cdc.gov)
  • HepG2 cells were used to further explore the underlying mechanisms in vitro . (karger.com)
  • Furthermore, the high refractoriness of liver cancer to treatments has been associated with the interaction of very complex and diverse mechanisms of chemoresistance, which can act synergistically to protect tumor cells from the chemotherapy agents [ 3 ] . (encyclopedia.pub)
  • The evidence for an apoptotic form of cell death caused by nyaope was less convincing with mixed findings observed for the markers (Bax/Bcl-2) studied. (wits.ac.za)
  • Several main apoptotic indicators were found in GS-002-treated cells, such as the cleaved forms of caspase-3, caspase-9, and poly(ADP-ribose) polymerase (PARP). (hindawi.com)
  • In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
  • Within this work, and considering the discussed background, another D. tertiolecta clone (CCMP 1320) was selected and tested against several cancer cell lines, and a bioactivity-guided fractionation approach allowed to identify the source of the biological activity observed within the extracts of this green alga. (frontiersin.org)
  • The two stable cell lines, Ishikawa-GFP (uterine origin) and MCF7-GFP (breast origin), displayed increased intracellular GFP intensity with estrogens. (cornell.edu)
  • The silicon microCCA device contained a co-culture of three cell lines: MCF7-GFP, Ishikawa-GFP and HepG2/C3A (liver origin). (cornell.edu)
  • BL5) in the aforementioned cell lines. (bvsalud.org)
  • We investigated 21 cell lines routinely used for virus isolation or research. (cdc.gov)
  • It also shows that SARS-CoV can achieve high titers in several cell lines, sometimes in the absence of specific cytopathic effects. (cdc.gov)
  • Simultaneously, these investigations showed that SARS-CoV could not replicate in a number of other cell lines routinely used for respiratory virus isolation. (cdc.gov)
  • Therefore, we investigated the susceptibility of a number of cell lines to SARS-CoV. (cdc.gov)
  • Because SARS-CoV is classified as a risk group level 4 pathogen in Australia, all procedures performed with the virus, including infecting cell lines and viral lysis before RNA extraction, were carried out in a physical containment level 4 (PC4) laboratory. (cdc.gov)
  • Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
  • Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
  • Systems toxicology, therefore, aims at understanding and exploring the way that different biological components are orchestrated as an ensemble in cells, tissues, and organisms. (brill.com)
  • These cells were derived from a variety of species and tissues and included those capable of supporting the replication of respiratory and enteric viruses. (cdc.gov)
  • The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)
  • mRNA expression analysis of PFOA exposed cells showed that cell cycle homeostasis genes were affected significantly, as well as the ten-eleven translocation methylcytosine dioxygenases (TETs) and the essential lipid metabolism genes. (illinois.edu)
  • Expression of OCT4 gene dropped after RA treatment, but it was recovered in NT2-RA-VH cells. (bvsalud.org)
  • 14] "The expression of the solute carriers NTCP and OCT-1 is regulated by cholesterol in HepG2 cells. (tcdb.org)
  • A subset of cancer cells undergoing EMT acquire characteristics of stem cells and are designated as cancer stem cells (CSCs) [ 8 ]. (techscience.com)
  • This article will examine advances taking place on both the lab bench and the pharmaceutical production floor that are helping to bring the promise of stem cells closer to reality. (ddw-online.com)
  • Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)
  • Inclusion of fetal bovine serum in stem cell media further contributes to an illdefined culture system. (ddw-online.com)
  • The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)
  • Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
  • Identifying the right cocktail of media conditions, supplements and growth factors that successfully drive stem cells toward a desired lineage on a reproducible basis is a time-consuming, iterative exercise. (ddw-online.com)
  • Further attention should be given to the understanding of additive, synergistic, and antagonistic effects of multiple mycotoxins in in vitro systems and implications for animal responses in vivo. (purdue.edu)
  • After infection with SARS-CoV, cells were observed for cytopathic effects, and quantitative real-time polymerase chain reaction was used to measure ongoing viral replication. (cdc.gov)
  • QJD showed beneficial effects, such as increased body weight, decreased fecal water content, and reduced inflammatory cell infiltration in the duodenum and colon, as well as maintaining the structure of the duodenum and colon. (bvsalud.org)
  • In this review, we summarize the current status and emerging trends in nanomedicine-based strategies targeting cancer immune evasion and modulating the immunosuppressive tumor microenvironment, including the inhibition of immunosuppressive cells in the tumor area, the activation of dendritic cells and the stimulation of the specific antitumor T-cell response. (unav.edu)
  • Poor oral bioavailability of chelidonine, a bio-active ingredient of Chelidonium majus, showing anti-cancer potentials against cancer cells with multidrug resistance, makes its optimal use rather limited. (bgu.ac.il)
  • Activating transcription factor-(ATF-) 3, a stress-inducible transcription factor, is rapidly upregulated under various stress conditions and plays an important role in inducing cancer cell apoptosis. (hindawi.com)
  • In breast cancer, ATF-3 enhances cancer cell-initiating features [ 22 ] and is associated with activation of the canonical Wnt/ β -catenin pathway [ 23 ]. (hindawi.com)
  • Among lung cancers, nonsmall cell lung carcinomas (NSCLC) account for approximately 80% of lung cancer cases [ 1 ]. (hindawi.com)
  • These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
  • A methodology to evaluate the efficacy of chelators to remove copper from the cell model was developed, and tested with a series of new copper chelating compounds. (sfu.ca)
  • In the HCC cells, activation of the NF-kappa B signaling pathway and IkB phosphorylation was significantly reduced by EIF5B silencing. (techscience.com)
  • CONCLUSION: QJD significantly increased body weight, decreased water content in feces, relieved inflammatory cell infiltration, maintained the structure of duodenum and colon. (bvsalud.org)
  • The use of this device as a biosensor was demonstrated by monitoring PEG encapsulated cells in microCCA exposed to a model toxicant, Triton X-100. (cornell.edu)
  • Both POE and PJ effectively reduced Disease Activity Index scores and inflammatory cell infiltration in the UC mouse model, but PJ had a better effect than POE. (bvsalud.org)
  • The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. (cancerindex.org)
  • Further, a photopolymerizable hydrogel based method was developed for direct patterning of multiple types of cells into their individual cell culture chambers in a PDMS microCCA. (cornell.edu)
  • Use of feeder layers requires two cell types to be maintained in parallel and introduces mouse proteins into the culture system. (ddw-online.com)
  • Progenitor cells can be far easier to handle in culture than ES cells. (ddw-online.com)
  • Twenty PCB 2 metabolites belonging to 13 metabolite classes, including five dechlorinated metabolite classes, were identified in the cell culture media from HepG2 cells exposed for 24 h to 10 M or 3.6 nM PCB 2. (cdc.gov)
  • Flowcytometry was performed to evaluate the cell cycle analysis of corneal keratocytes. (blogspot.com)
  • Cell cycle analysis with PI method also showed that the numbers of cells at the S phase of the cell cycle in these cells were increased. (bvsalud.org)
  • EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
  • Previous studies reported that ATF-3 was induced by treating cells with antitumorigenic compounds [ 14 - 18 ] and a phosphoinositide 3-kinase inhibitor [ 19 ]. (hindawi.com)
  • Subsequent studies were conducted to determine the relationship between in vitro induction data and in vivo induction response. (aspetjournals.org)