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HEPESSaline Solution, HypertonicSaltsSodium ChlorideSodiumSolutionsBuffersHydrogen-Ion ConcentrationSulfonic AcidsTromethamineBicarbonatesIsotonic SolutionsAmino Acids, NeutralSodium AcetatePhosgeneRats, WistarTasteSurface-Active AgentsDose-Response Relationship, DrugSodium ChannelsRats, Sprague-DawleyRabbitsSodium OxybateAnionsWaterChloridesTime FactorsChorda Tympani NerveOsmolar ConcentrationKineticsPotassiumPlasma SubstitutesRandom AllocationChemistry4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidChemical PhenomenaDihydroorotate OxidaseMagnetic Resonance SpectroscopyCattle
ChlorideGlucosePyruvateBicarbonatePhosphate bufferIsotonicCitrateMagnesiumNaClSterileConcentrationGlacial acetHBSSEDTAPhysiologicalFormaldehydeFormalinMolecular biologyPreparationChemicallyReagents2020OsmoticAcidicVitroGlutamineCurrentsAcrylamideRetrievalElectrophoresisBloodNucleicExperimentsCapacityCellNormalAmountMediaTissueWaterMedicalMaterials
Chloride7
Glucose4
Pyruvate2
  • Without sodium pyruvate and sodium bicarbonate. (caissonlabs.com)
  • Alpha-MEM medium (aMEM) is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, thioctic acid, vitamin B12, biotin, and ascorbic acid. (biowest.net)
Bicarbonate6
Phosphate buffer1
  • 50% skim milk in 0.01 M phosphate-buffered saline. (hl7.org)
Isotonic7
  • Less commonly, this solution is referred to as physiological saline or isotonic saline (because it is approximately isotonic to blood serum, which makes it a physiologically normal solution). (wikipedia.org)
  • However, if the osmotic coefficient (a correction for non-ideal solutions) is taken into account, then the saline solution is much closer to isotonic. (wikipedia.org)
  • Sterile isotonic saline is also used to fill breast implants for use in breast augmentation surgery, to correct congenital abnormalities such as tuberous breast deformity, and to correct breast asymmetry. (wikipedia.org)
  • It is an isotonic buffered saline solution commonly used in biology, such as experiments in immunohistochemistry, situ hybridization, enzyme-linked immunosorbent assay. (vacutaineradditives.com)
  • TBS buffer is a salt buffer made by isotonic salt solution and TRIS-HCL buffer,mainly composed of TRIS-HCL, NaCl, tween20.The suitable PH range is 7.2-7.5. (vacutaineradditives.com)
  • Tyrodes solution is a solution that is roughly isotonic with interstitial fluid and used in physiological experiments and tissue culture. (web.app)
  • This is an isotonic, balanced salt solution. (severnbiotech.com)
Citrate1
Magnesium2
  • Ringer experimented with various solutions containing the chlorides of sodium, potassium, calcium and magnesium, in order to obtain a suitable physiological saline solution which would keep the heart. (web.app)
  • The 19th-century English physiologist Sydney Ringer developed salt solutions containing the chlorides of sodium, potassium, calcium and magnesium suitable for maintaining the beating of an isolated animal heart outside the body. (wikipedia.org)
NaCl4
  • Normal saline (NSS, NS or N/S) is the commonly used phrase for a solution of 0.90% w/v of NaCl, 308 mOsm/L or 9.0 g per liter. (wikipedia.org)
  • Since normal saline contains 9 grams of NaCl, the concentration is 9 grams per litre divided by 58.4 grams per mole, or 0.154 mole per litre. (wikipedia.org)
  • Feces obtained from the sheep are suspended in water, and saturated NaCl solution was used for washing them through the sieve. (techagro.org)
  • Wash the transfected cells twice with tyrodes solution 7 mm nacl, 2. (web.app)
Sterile1
  • It is most commonly used as a sterile 9 g of salt per litre (0.9%) solution, known as normal saline. (wikipedia.org)
Concentration1
  • Second, the effect of TRIS buffer is changed by temperature,for example, at room temperature of 25°C, the PH of TRIS buffer is 7.8,but 8.4 when it's reached at 4 °C and 7.4 at 37°C. Therefore, when the buffer is deployed at 4°C,but the test temperature is at 37°C, the results of its hydrogen ion concentration will be increase tenfold. (vacutaineradditives.com)
Glacial acet1
  • Picric acid, saturated aqueous solution (750.0 ml), 37-40% formalin (250.0 ml), glacial acetic acid (50.0 ml). (hl7.org)
HBSS1
EDTA4
  • TE buffer is a kind of electrophoresis buffer,prepared by TRIS and EDTA. (vacutaineradditives.com)
  • TAE buffer is a buffer composed of Tris, acetic acid and EDTA. (vacutaineradditives.com)
  • TBE buffer is a solution composed of Tris base, boric acid and EDTA (ethylenediaminetetraacetic acid). (vacutaineradditives.com)
  • 50-100 ng/µl in TE Buffer (10 mM Tris-CL, 0.5 mM EDTA, pH 8.0). (cls.shop)
Physiological2
  • Chemically defined, Balanced Salt Solutions provide an environment that will maintain the structural and physiological integrity of mammalian cells in vitro. (jainbiologicals.com)
  • Physiological salt solution also called as pss ringer solution maintains tissue outside the animal body select pss in which tissue last longer prepare the solution with the help of distilled. (web.app)
Formaldehyde1
  • A 10% v/v solution in water of formalin( a 37% solution of formaldehyde and water). (hl7.org)
Formalin1
  • A modification of buffered 10% formalin used as a general tissue preservative. (hl7.org)
Molecular biology1
  • Ringer tyrode solution in oxford dictionary of biochemistry and molecular biology 2 length. (web.app)
Preparation1
  • This invention relates to the hydrochloride salt of 5-[3-(3-hydroxyphenoxy)azetidin-1-yl]-5-methyl-2,2-diphenylhexanamide and to processes for the preparation of, intermediates used in the preparation of, compositions containing and the uses of, said compound. (justia.com)
Chemically2
  • hOAT3 interacted with chemically heterogeneous anionic compounds, such as nonsteroidal anti-inflammatory drugs, diuretics, sulfobromophthalein, penicillin G, bile salts and tetraethyl ammonium bromide. (aspetjournals.org)
  • Tyrodes solution acidified facilitates assisted hatching by chemically drilling the zona pellucida. (web.app)
Reagents2
  • Hubei Xindesheng Material Co.,ltd is a high-tech company specializing in the research and development, production and sales of biological buffers, blood collection tube additives, chemiluminescence reagents and luminescent substrates. (vacutaineradditives.com)
  • Usa home product directory cell culture classical media and salts balanced salt mixtures and solutions tyrode s salts 3d cell culture cell culture reagents. (web.app)
20201
  • In 2020, sodium was the 274th most commonly prescribed medication in the United States, with more than 1 million prescriptions. (wikipedia.org)
Osmotic1
  • In those with long-standing low blood sodium, excessive use may result in osmotic demyelination syndrome. (wikipedia.org)
Acidic2
  • Saline is acidic, with a pH of 5.5 (due mainly to dissolved carbon dioxide). (wikipedia.org)
  • T1788 tyrodes solution, acidic email this page to a friend. (web.app)
Vitro3
  • Sodium channel β1 subunits modulate α subunit gating and cell surface expression and participate in cell adhesive interactions in vitro . (jneurosci.org)
  • β1 subunits affect sodium channel gating and cell surface expression when expressed in heterologous cells in vitro . (jneurosci.org)
  • Bosentan, sodium salt (lot 71102B1758) used for intravenous dosing and in vitro experiments, bosentan, micronized free sulfonamide (lot 704004) for oral pharmacokinetic experiments, and 14 C-radiolabeled bosentan, free sulfonamide (specific activity 40.9 μCi/mg, lot 12352B65) were obtained from Actelion Pharmaceuticals Ltd. (Allschwil, Switzerland) internal sources. (aspetjournals.org)
Glutamine1
Currents2
  • Sodium currents in dissociated hippocampal neurons are normal, but Na v 1.1 expression is reduced and Na v 1.3 expression is increased in a subset of pyramidal neurons in the CA2/CA3 region, suggesting a basis for the epileptic phenotype. (jneurosci.org)
  • I would recommend a naatp solution or trisatp solution since mg can be a blocker at nmda currents and likely affect epscs. (web.app)
Acrylamide1
  • No. 4,481,009, in which the first monomer solution comprised acrylic acid, and the only monomer in the second monomer solution was acrylamide, became calcified after being subjected to mechanical flexing and then implanted. (justia.com)
Retrieval1
  • Dako target retrieval solution, ph 9 x 10 from dako 5. (web.app)
Electrophoresis4
  • With low ionic strength of Tris buffer ,it can be used for the formation of intermediate fibers of lamin in C. elegans.Tris is also one of the main components of protein electrophoresis buffer. (vacutaineradditives.com)
  • It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH during electrophoresis. (vacutaineradditives.com)
  • It's a buffer widely used for short-term electrophoresis of large fragments of DNA. (vacutaineradditives.com)
  • TBE buffer is commonly applied in agarose gel electrophoresis to analyze DNA products resulting from PCR amplification, DNA purification protocols, or DNA cloning. (vacutaineradditives.com)
Blood1
  • Although neither of those names is technically accurate because normal saline is not exactly like blood serum, they convey the practical effect usually seen: good fluid balance with minimal hypotonicity or hypertonicity. (wikipedia.org)
Nucleic2
  • Tris, also known as Tris (Hydroxymethyl) Aminomethane, Tris buffer is widely used, especially in nucleic acid and research experiments related to DNA, it has much more advantages than PBS phosphate buffer. (vacutaineradditives.com)
  • At the same time, because it does not precipitate with metals,and with little interference with biochemical processes,tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many other important uses. (vacutaineradditives.com)
Experiments1
  • In biochemical experiments and related research work, a large amount of buffer is required to maintain the PH of the testing system. (vacutaineradditives.com)
Capacity2
  • At last, the buffering capacity is poor when the PH is lower than 7.5. (vacutaineradditives.com)
  • The ileum was cut into 2 inches pieces6 and transferred to an organ bath of 50ml capacity containing tyrodes solution in mm. (web.app)
Cell2
  • These results suggest that effects of β1 on sodium channel gating are dependent on the genetic background and signal transduction pathways present in the cell type used in heterologous expression and emphasize the importance of analyzing the effects of β1 in vivo . (jneurosci.org)
  • From buffer solutions to cell culture media formulations at bench top or production sales, Caisson Labs gives you the products you need in a way that makes sense to you. (caissonlabs.com)
Normal3
  • Normal saline will not burn or sting when applied. (wikipedia.org)
  • The amount of normal saline infused depends largely on the needs of the person (e.g. ongoing diarrhea or heart failure). (wikipedia.org)
  • Our results show that β1 subunits play important roles in the regulation of sodium channel density and localization, are involved in axo-glial communication at nodes of Ranvier, and are required for normal action potential conduction and control of excitability in vivo . (jneurosci.org)
Amount1
  • Buffer solution is usually a solution composed of weak acid and its conjugate base or weak base and its conjugate acid , which can change PH when a certain amount of other substances are added. (vacutaineradditives.com)
Media1
  • The effect of trisbuffered media and tyrode physiologic saline solution on the antigenic release of histamine from human leukocytes tadao okazaki, m. (web.app)
Tissue1
  • [6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
Water1
  • citation needed] Saline is also used in I.V. therapy, intravenously supplying extra water to rehydrate people or supplying the daily water and salt needs ("maintenance" needs) of a person who is unable to take them by mouth. (wikipedia.org)
Medical3
  • The medical use of saline began around 1831. (wikipedia.org)
  • For medical purposes, saline is often used to flush wounds and skin abrasions. (wikipedia.org)
  • Tyrode solution definition of tyrode solution by medical. (web.app)
Materials1
  • Among them, TRIS and its derivative buffers are widely used in organic synthesis, coatings, fireproof materials, and biochemical and pharmaceutical fields. (vacutaineradditives.com)