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  • substrates
  • HEPES is the buffer of choice in a protein deposition technique in electron microscopy because it did not affect metal substrates. (mpbio.com)
  • 2) Stability of the buffer, whether it interacts with the substrates, cofactors, or metal ions, the temperature coefficients of its pK, the ionic strength at which it is used, its absorbance in the UV region of the spectrum, its cost, and its availability free from contaminants. (openwetware.org)
  • suitable
  • HEPES has been evaluated and shown to be suitable for use with Ampholines in generating pH gradients less than 1 pH unit wide for isolectric focusing applications. (mpbio.com)
  • Standard physiological buffer that is suitable for various calcium imaging experiments. (fishersci.com)
  • solutions
  • Studies have indicated that 20 mM HEPES is the most satisfactory concentration of the buffer when both Hanks' and Earle's solutions are used. (mpbio.com)
  • For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. (promocell.com)
  • Range
  • Buffer strength for cell culture applications is usually in the range of 10 to 25 mM. (mpbio.com)
  • Buffering pH range 6.8 - 8.2. (p212121.com)
  • The paper examines, twelve new or little used hydrogen ion buffers (at the time) covering the range pKa = 6.15-8.35 have been prepared and tested. (p212121.com)
  • good
  • HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance. (mpbio.com)
  • Applications
  • The anode buffer container (ABC) contains 1x running buffer to support all electrophoresis applications on the Applied Biosystems 3500 series genetic analyzers. (fishersci.com)
  • culture
  • Buffer for use in plant tissue culture. (thomassci.com)
  • Use approximately 15-120 µl Neuroporter™ Transfection Reagent and 6-8 µg DNA (in provided unique DNA Dilution buffer when required) per 6 cm cell culture plate. (thomassci.com)
  • shown
  • As shown in Figures 3D and 3E, ADPR hydrolysis is significantly affected also by the buffering species present in the reaction mixture. (ctskinhibito.com)