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  • proteoglycan
  • Cell surface heparan sulfate proteoglycan syndecan-2 induces the maturation of dendritic spines in rat hippocampal neurons. (nih.gov)
  • We demonstrate that the cell surface heparan sulfate proteoglycan syndecan-2 plays a critical role in spine development.Deletion of the COOH-terminal EFYA motif of syndecan-2, the binding site for PDZ domain proteins, abrogates the spine-promoting activity of syndecan-2.Our results indicate that syndecan-2 plays a direct role in the development of postsynaptic specialization through its interactions with PDZ domain proteins. (nih.gov)
  • We demonstrate that the cell surface heparan sulfate proteoglycan syndecan-2 plays a critical role in spine development. (nih.gov)
  • synapsin
  • Confocal microscopy revealed close apposition of cell surface heparan sulfate and synapsin I immunoreactivities (Fig. 2, A-E). Frequently they showed partial, but not complete, overlap (Fig. 2, B and C). This staining pattern suggests that cell surface heparan sulfate is associated with the synaptic junctions of cultured hippocampal neurons. (nih.gov)
  • substrate
  • Although undegraded heparan sulfate is the primary stored substrate, glycolipids such as gangliosides are also stored despite no genetic defect in the enzymes associated with their breakdown. (wikipedia.org)
  • whereas
  • These and other studies led to the proposal that heparan sulfate may be involved in the cell-cell recognition phenomena and control of cell growth, whereas heparin may be involved in defense mechanisms against bacteria and other foreign materials. (naver.com)
  • similar
  • On the other hand, syndecan-2 is expressed in neurons in a time course and a pattern similar to those of heparan sulfate immunoreactivity. (nih.gov)
  • cell
  • Cell surface heparan sulfate immunoreactivity is detected on the cell bodies and along dendrites of the pyramidal neurons. (nih.gov)
  • D and E) High-power confocal image shows cell surface heparan sulfate immunoreactivity (yellow) as a puncta on the surface of MAP2-positive dendrites (red), in patterns and shapes reminiscent of dendritic spines. (nih.gov)
  • Double staining for heparan sulfate and either MAP2, a dendritic marker (Fig. 3, A-E), or NCAM (Fig. 3, F-H) further demonstrated a punctate distribution of cell surface heparan sulfate along the dendrites of hippocampal neurons. (nih.gov)
  • Further
  • Double labeling of methanol-fixed 30 DIV hippocampal neurons with antibodies to heparan sulfate and PSD-95 further confirmed the synaptic localization of heparan sulfate. (nih.gov)
  • 1998). These observations further support our finding that syndecan-2 is one of the HSPGs responsible for the spine-specific accumulation of heparan sulfate on mature hippocampal neurons in vitro, though a contribution by unknown HSPGs has not been ruled out. (nih.gov)
  • Double
  • Bar, 20 μm in A and 10 μm in D and E. (F-H) Double immunostaining of 30 DIV hippocampal neurons with anti-NCAM (red) and anti-heparan sulfate 10E4 (green) antibodies. (nih.gov)
  • cells
  • A glycosaminoglycoside (GAG) composition predominantly of tetrasacchardie units derived from heparin/heparan sulfate has enchanced activity in preventing the proliferation of smooth muscle cells. (google.com)