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  • gene expression
  • Abstract -Estrogen administration to postmenopausal women has been shown to increase plasma levels of apolipoprotein (apo) A-I. A human hepatoma cell line, Hep G2, was used to test the hypothesis that estrogen increases the hepatic production of apo A-I by modulating gene expression. (ahajournals.org)
  • Therefore, the aim of this study was to characterize the effect of low concentration of ethanol on gene expression profiling in HepG2 cells using cDNA microarrays with especial interest in genes with transcriptional and translational function. (medsci.org)
  • The gene expression pattern observed in the ethanol-treated HepG2 cells revealed a relatively similar pattern to that found in the untreated control cells. (medsci.org)
  • The cDNA microarray technique has been used to evaluate the global gene expression in HCC as well as HCC-derived cell lines [ 13 - 16 ]. (medsci.org)
  • Therefore, the aim of this study was to identify the effect of low concentration of ethanol (1 mM for 6 h) on gene expression, specifically from genes with transcriptional and translational function, in HepG2 cells compared to HepG2 cells not exposed to ethanol (control cells) using cDNA microarrays. (medsci.org)
  • twofold
  • Cells exposed to 10 μM Cu exhibited a 22-fold increase in Cu content and a twofold decrease in Fe content compared with cells maintained in 0.4 μM Cu. 64 Cu uptake in Cu-deficient Hep-G2 cells showed a twofold decrease in K m compared with cells grown in 10 μM Cu. The decreased K m may represent an adaptive response to Cu deficiency. (physiology.org)
  • Further, Ccn-BNP-GA showed an approximately twofold higher rate of cell apoptosis than the other groups. (nih.gov)
  • liver
  • 1 2 3 This inverse association is present in both men and women and is due to the important role played by HDL in the reverse cholesterol transfer pathway, in which HDL functions as an acceptor of excess cholesterol from peripheral cells and transports it back to the liver for removal. (ahajournals.org)
  • 9 12 13 In humans, the apo A-I gene is expressed in both liver and intestine, and studies in Hep G2 cells, a human hepatoma cell line, have demonstrated that estrogen can increase apo A-I concentration in the medium of these cells in a dose-dependent manner. (ahajournals.org)
  • The culture system described provides a basis for studying the regulation of hepatocyte-specific functions by soluble factors (either plasma- or cell-derived) and cell-substratum interactions in a human liver cell line. (biologists.org)
  • Biotin requirements are lower in human Jurkat lymphoid cells but homeostatic mechanisms are similar to those of HepG2 liver cells. (biomedsearch.com)
  • Liver cells are supplied through the portal vein with high levels of water-soluble vitamins compared with those of peripheral tissues. (biomedsearch.com)
  • Acute liver injury upregulates microRNA-491-5p in mice, and its overexpression sensitizes Hep G2 cells for tumour necrosis factor-alpha-induced apoptosis. (escholarship.org)
  • Methods: C57Bl/6 mice were administered anti-fas receptor antibodies to induce liver cell apoptosis. (escholarship.org)
  • CONCLUSIONS: One of the miRNAs that is associated with the acute liver injury mouse model, miRNA-491_5p, sensitizes Hep G2 cells for TNF-alpha-induced apoptosis, at least in part, by inhibiting AFP, hsp-90 and NF-kappaB. (escholarship.org)
  • 2) Use 3% pentobarbital anesthetized nude mice (SPF Balb/C nude mice, male, 4~5 weeks, 15g~20g), expose the liver by opening the abdomen, inject liver cancer cells into nude mice's liver, stitch wounds and place the nude mice in a heating pad, feed them after they were awake. (cloud-clone.us)
  • Use 3% pentobarbital anesthetized nude mice (SPF Balb/C nude mice, male, 4~5 weeks, 15g~20g), liver cancer cells were injected under the skin of the nude mice. (cloud-clone.us)
  • apoptotic
  • A competitive binding experiment was also conducted, and the results revealed that the apoptotic cells was gradually reduced by 35.12% when compared with Ccn-BNP-GA. These observations infer that Ccn-BNP-GA preferentially targeted GA receptors on the HepG2 cells. (nih.gov)
  • Evaluation of apoptosis in HepG2 cells was carried out by (a) microscopic observations of cell morphology, (b) DNA fragmentation analysis, (c) activities of caspase 3 and 9, as well as by (d) analysis of the expression of pro-apoptotic (Bax) and anti-apoptotic (Bcl-2) proteins associated with cell death. (biomedcentral.com)
  • Cell apoptosis was assessed by Annexin V-FITC/PI staining: all three cancer cells treated with karanjin exhibited significantly increased apoptotic rates, especially in the percentage of late apoptosis cells. (uga.edu)
  • protein
  • Our data indicate that, as a result of Cu overload, Hep-G2 cells reduced their Fe content and their DMT1 protein levels. (physiology.org)
  • Berberine metabolites exhibit triglyceride-lowering effects via activation of AMP-activated protein kinase in Hep G2 cells. (nih.gov)
  • Among those BBR metabolites, M2 exhibited the more potential effects on TG-lowering and AMP-activated protein kinase (AMPK) activation in Hep G2 cells as compared with BBR. (nih.gov)
  • Hep G2 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. (genetex.com)
  • Treatments of mitochondrial inhibitors and an uncoupler respectively reduced both the protein level and transactivation activity of HIF-1α in HepG2 cells under normoxia or hypoxia. (nih.gov)
  • Mitochondrial dysfunctions resulted in reduced HIF-1α protein synthesis through AMPK-dependent manner in HepG2 cells. (nih.gov)
  • Overexpression of microRNA-491_5p sensitized Hep G2 cells for TNF-alpha-induced apoptosis, and also caused an inhibition of alpha-fetoprotein, (AFP), heat shock protein-90 (hsp-90) and nuclear factor-kappaB (NF-kappaB). (escholarship.org)
  • tissue culture
  • To transfect Hep G2 cells in different tissue culture formats, vary the amounts of Lipofectamine® LTX Reagent, DNA, cells, medium and PLUS™ Reagent used in proportion to the relative surface area, as shown in the table (amounts given on a per well basis). (thermofisher.com)
  • This contrasted with the pattern of growth of cells cultured in the presence of serum on type I collagen gels and cells cultured on tissue-culture plastic in either CDM or medium containing serum which formed foci of multilayered cells. (biologists.org)
  • membrane
  • Apolipoprotein B-100 (apoB-100) appears in three forms in the endoplasmic reticulum of Hep G2 cells: (1) tightly bound to the membrane, ie, not extractable by sodium carbonate. (ahajournals.org)
  • In the current work, adenoviral infection with vectors that resulted in expression of catalase either in the cytosol or mitochondrial compartments was able to abolish the loss of mitochondrial membrane potential or damage to mitochondria observed in HepG2 cells overexpressing CYP2E1 that were treated with BSO. (aspetjournals.org)
  • humans
  • Because studies in humans were limited, other investigators examined disposition, metabolism, and induction potential of chrysin in clinical studies or immortalized human cell lines. (aspetjournals.org)
  • hepatic
  • In conclusion, E 2 increases apo A-I production in hepatic cells by increasing the transcription of the apo A-I gene. (ahajournals.org)
  • AIMS: The aim of this study was to evaluate the role of a specific miRNA in modulating hepatic cell functions. (escholarship.org)
  • The role of miRNA-491_5p, which was overexpressed in the model, in modulating hepatic cell functions was evaluated. (escholarship.org)
  • oxidative damage
  • In this study, the ability of the water extracts of the leaf (BLE) and stem (BSE) from the shoots to protect HepG2 cells against oxidative damage was studied. (peerj.com)
  • Antioxidant analyses of B. racemosa using cellular model has never been conducted and information obtained from such study can provide useful data particularly with regards to their ability to protect cells against oxidative damage. (peerj.com)
  • CYP2E1
  • TPA did not lower but instead induced catalytically active CYP2E1 in these cells. (aspetjournals.org)
  • Previous results reported that depletion of GSH by buthionine-( S , R )-sulfoximine (BSO) treatment caused apoptosis and necrosis in HepG2 cells, which overexpress CYP2E1. (aspetjournals.org)
  • As previously described, high levels of catalase can be expressed in HepG2 cells overexpressing CYP2E1 by using adenovirus-mediated gene transfer ( Bai and Cederbaum, 2001 ). (aspetjournals.org)
  • receptor
  • Expression of the LDL receptor and HMG-CoA (3-hydroxy-3-methylglutaryl coenzyme A) reductase genes was comparable in Hep G2 cells cultured in CDM and serum-containing medium. (biologists.org)
  • The brown rice bio-active peptide illustrate various functions such as Polymorphonuclear cells stimulation, activation of C3a (one of the complement system components) receptor (C3aR) and the ileum contraction [1- (medcraveonline.com)