Hematopoietic stem vitro embryonic stem cell differentiation. Medical search. Frequent questions

Identification of genes involved in in vitro differentiation induction of embryonic stem cells (ESCs) into hematopoietic stem cells (HSCs) has been challenged during last decade. (ebiotrade.com)
Hematopoietic stem cells (HSCs) emerge from hemogenic endothelial (HE) cells, through endothelial-to-hematopoietic transition (EHT). (lu.se)
However, metabolic processes underlying the emergence of HSCs from HE cells remain unclear. (lu.se)
Although the use of hematopoietic stem cells (HSCs) for therapeutic purposes has been widely applied in clinic 11-13 , HSCs have reduced differentiation and proliferative capacities, and HSCs are difficult to expand in in vitro cell culture 14-16 . (psu.edu)
Thus, iPS cells have greater potential to be used in ACT-based immunotherapy compared to ESCs or HSCs. (psu.edu)
The major shortcoming in generating functional hematopoietic stem cells (HSC) from pluripotent stem cells is the lack of adequate markers for specification and maintenance of functional human HSCs. (ca.gov)
Using these reporter lines to monitor the induction of HSC "stemless' program, we are making progress in optimizing differentiation conditions toward generating functional HSCs for transplantation. (ca.gov)
Several HOX transcription factors, including HOXB4, are highly expressed in hematopoietic stem cells (HSCs) but are downregulated upon differentiation towards more mature progeny cells. (dissertations.se)
We demonstrate that transplantation of fetal hematopoietic stem cells (HSCs) made vitamin D deficient in utero induce diabetes in vitamin D-sufficient mice. (bvsalud.org)
Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
moreover, they also promote the hematopoietic differentiation of mouse ESCs and favor the development of definitive HSCs from pre-HSCs in the mouse aorta-gonad-mesonephros (AGM) region [ 8 ]. (biomedcentral.com)
Hematopoietic stem cells (HSCs) are a rare hematopoietic cell type that can entirely reconstitute the blood and immune systems following transplantation. (stanford.edu)
Hematopoietic stem cells (HSCs), first produced in the developing vertebrate embryo, supply the lifelong foundation of the blood and immune systems. (grantome.com)
Likewise, while the number of transplanted HSCs is well known to directly impact engraftment efficiency, there are currently no established clinical protocols to successfully expand donor-harvested HSCs, nor to differentiate embryonic or induced pluripotent stem cells (iPSCs) into functional HSCs in vitro. (grantome.com)
Therefore, the identification of novel modifiers of de novo production of HSCs with long-term self-renewal and differentiation capacity is a major unmet clinical need. (grantome.com)
transcriptional program, however transplantation of these in vitro-derived HSCs into irradiated mice illustrates both limited long-term engraftment and multilineage potential. (grantome.com)
In the developing embryo, definitive HSCs arise from a unique population of mesodermal precursors termed hemogenic endothelium (HEC) through a process known as endothelial-to-hematopoietic transition (EHT). (grantome.com)
Defining the molecular signaling pathways that mediate productive HSC formation in vivo will reveal new targets for optimizing the directed expansion and/or differentiation of adult-type HSCs for clinical use. (grantome.com)
Hematopoietic stem cells (HSCs), produced in the developing embryo, form the foundation of our blood system. (grantome.com)
This work has significant relevance for the in vitro production and expansion of functional HSCs for therapeutic use. (grantome.com)

The hematopoietic and endothelial lineages derive from mesoderm and are thought to develop through the maturation of a common progenitor, the hemangioblast. (elsevierpure.com)
Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)
Recently, stage-specific embryonic kidney progenitor tissue was used to regenerate nephrons. (tau.ac.il)
Through fluorescence-activated cell sorting, microarray analysis, in vitro differentiation assays, mixed lymphocyte reaction, and a model of ischemic kidney injury, this study sought to identify and characterize multipotent organ stem/progenitor cells in the adult kidney. (tau.ac.il)
Genetics is closely attributed to Progenitor cell in his work. (research.com)
In his study, Pablo Menendez carries out multidisciplinary Progenitor cell and Embryonic stem cell research. (research.com)
However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)
Single-cell transcriptomic analysis confirmed inhibition of lineage-committed progenitor cells in physioxic cultures. (stanford.edu)
To overcome this issue, we established immortalized megakaryocyte cell lines (imMKCLs) by introducing three transgenes, c-MYC, BMI1, and BCL-XL, sequentially into hematopoietic and megakaryocytic progenitor stage cells derived from iPSCs. (biomedcentral.com)
We demonstrate that neural stem/progenitor cell (NSPC) self-renewal and spatiotemporal generation of neurons and other cell types are severely impacted by the loss of Ythdf2 in embryonic neocortex. (biomedcentral.com)
Hematopoietic stem and progenitor cells (HSPCs) that are essential for establishment and maintenance of the adult blood system are generated during embryonic definitive hematopoiesis. (hirschilabuva.com)

In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
Lei said that in the next phase, the research team needs to conduct comprehensive detection and analysis of this group of hematopoietic stem cells, and screen out the relevant genes affecting the early hematopoiesis differentiation of human pluripotent stem cells in the space environment by comparing with the ground control group. (pdnews.cn)
Our studies also suggest that these hESC reporter lines will become valuable for investigators working on optimizing stem cell differentiation and culture for other tissues, as many of these reporter genes are not only restricted to HSC. (ca.gov)
The transcriptional activator Put3p allows yeast cells to utilize proline as a nitrogen source through expression of the PUT1 and PUT2 genes. (openrepository.com)
Here we show that FOS and JUN together bind to and activate a core set of vascular genes in the hemogenic endothelium and that upon global inhibition of AP-1 by expression of a dominant negative FOS peptide the balance between endothelial and hematopoietic fate is shifted towards blood. (nih.gov)
RNA sequencing was measured to identify the differential expressed genes due to loss of Sirt6 in somatic and pluripotent cells. (biomedcentral.com)
At 48 hours, after differentiation into vascular endothelium was determined, a system of transcription was established in which genes unique to vascular endothelial differentiation were induced. (medicalxpress.com)
Han Y , He X , Lee S , Mao Y , Liu X , Sun H , Jin M , Kwon T , . Network analysis for the identification of hub genes and related molecules as potential biomarkers associated with the differentiation of bone marrow-derived stem cells into hepatocytes. (aging-us.com)
Herein, we screened differentially expressed genes between hepatocytes and untreated BMSCs to identify the genes responsible for the differentiation of BMSCs into hepatocytes. (aging-us.com)
Hub genes were analyzed using the STRING and Cytoscape v 3.8.2, revealing that twenty-four hub genes, play a pivotal role in the differentiation of BMSCs into hepatocytes. (aging-us.com)
Expression trapping: identification of novel genes expressed in hematopoietic and endothelial lineages by gene trapping in ES cells. (ox.ac.uk)
Using two different gene trap vectors, we have isolated embryonic stem (ES) cell clones containing lacZ reporter gene insertions in genes expressed in blood island and vascular cells, muscle, stromal cells, and unknown cell types. (ox.ac.uk)
Expression and sequence analysis of representative clones suggest that this approach will be useful for identifying and mutating novel genes expressed in the developing hematopoietic and vascular systems. (ox.ac.uk)
By following the division and differentiation of stem cells and studying the related genes, scientists can better understand how a complex organism develops from a fertilized egg, as well as the gene functions in different stages of embryo developmental. (creative-bioarray.com)
The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
Induces upregulation of the pluripotency network genes (Klf4, Klf2, Nanog, Oct4, Myc, Sox2) and epigenetic-associated proteins (Dnmt3a, Mbd3) in mouse ES cells, and inhibits retinoic acid-induced differentiation (Adamo et al. (stemcell.com)
Found within Wharton's jelly - which is easily harvested from what would otherwise be post-natal medical waste - are several distinct stem cell genes. (articlecity.com)
The HOX family of genes plays a major role in embryogenesis and cell differentiation, including differentiation of hematopoietic cell lines. (medscape.com)

The cell line is pluripotent and can differentiate into cell types of all three germ layers. (wikipedia.org)
Also, exposing P19 cells to retinoic acid (RA) can differentiate them into neuronal cells. (wikipedia.org)
The difference between these subtype cell lines is the ability to differentiate into neuronal cells or muscle cells in response to treatment with retinoic acid or DMSO, respectively. (wikipedia.org)
Because embryonal carcinoma can differentiate into cells of all three germ layers, P19 cells can also differentiate into those ectoderm, mesoderm and endoderm-like cells. (wikipedia.org)
When embryonal carcinoma cells are cultured at high density, they start to differentiate. (wikipedia.org)
Studies have shown that a certain concentration of RA can induce P19 cells to differentiate into neuronal cells, including neurons and glial cells, whereas 0.5% - 1% DMSO led P19 cells to differentiate into cardiac or skeletal muscle cells. (wikipedia.org)
Those studies showed that drug exposure causes multipotent P19 cells to differentiate into different layers of cells. (wikipedia.org)
Other than into neurons and astrocytes, P19 cells can also differentiate to oligodendrocytes, which can be detected using the specific markers, myelin-associated glycoprotein and 2',3'-Cyclic-nucleotide 3'-phosphodiesterase. (wikipedia.org)
In addition, like ESCs, iPS cells possess indefinite proliferative capacity in vitro, and have been shown to differentiate into hematopoietic cells. (psu.edu)
This stem cell will further mature and differentiate to form a group of hematopoietic stem cells resembling a cluster of grapes. (pdnews.cn)
They found that the histone code, which alters the transcriptional state of the gene, changes over time as stem cells differentiate into blood vessels in response to a stimulus. (medicalxpress.com)
Eventually, the cells begin to differentiate, taking on a certain function in a part of the body. (healthline.com)
Adult stem cells can't differentiate into as many other types of cells as embryonic stem cells can. (healthline.com)
They can differentiate into all types of specialized cells in the body. (healthline.com)
The breakthrough has created a way to "de-differentiate" the stem cells. (healthline.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
They are able to differentiate into any cell of an organism and have the ability of self-renewal. (articlecity.com)
Adult stem cell -An undifferentiated cell found in a differentiated tissue that can renew itself and (with certain limitations) differentiate to yield all the specialized cell types of the tissue from which it originated. (cellmedicine.com)
Cell-based therapies -treatment in which stem cells are induced to differentiate into the specific cell type required to repair damaged or depleted adult cell populations or tissues. (cellmedicine.com)
Stem cells (SCs) are undifferentiated cells with self-renewal ability and capacity to differentiate into specialized cell types. (bvsalud.org)
Due to the ability to self-renew and to differentiate into cells that are found throughout the body, there is a great interest in using stem cells for the regeneration of injured tissues as well as to develop tissue-engineered implants and bio-hybrid organs, in order to restore tissue function. (bvsalud.org)

Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)
The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
In addition, these cells are enriched for β1-integrin, are cytokeratin negative, and show minimal expression of surface markers that typically are found on bone marrow-derived mesenchymal stem cells. (tau.ac.il)
In his works, Pablo Menendez performs multidisciplinary study on Mesenchymal stem cell and Stem cell. (research.com)
Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
Stem cells, such as hematopoietic stem cells and bone marrow mesenchymal stem cells, can also be used as carriers of gene therapy for disease treatment. (creative-bioarray.com)
Inhibits proliferation and promotes osteogenic differentiation, while inhibiting adipogenic differentiation, of human amnion-derived mesenchymal stem cells (MSCs) and rabbit bone marrow-derived MSCs (Wu et al. (stemcell.com)
Mesenchymal stem cells -Cells from the immature embryonic connective tissue. (cellmedicine.com)
A number of celltypes come from mesenchymal stem cells, includingchondrocytes, which produce cartilage. (cellmedicine.com)
Here, by developing injectable, void-forming hydrogels that decouple pore formation from elasticity, we show that mesenchymal stem cell (MSC) osteogenesis in vitro , and cell deployment in vitro and in vivo , can be controlled by modifying, respectively, the hydrogel's elastic modulus or its chemistry. (nature.com)
Mesenchymal stem cells (MSCs) are ASCs, and were first described in 1966 by Friedenstein et al. (bvsalud.org)

Adenoviral mediated overexpression of HOXB4 in human primitive hematopoietic cells in vitro generates very high levels of HOXB4 within 24 hours of transduction, leading to an increased differentiation towards myeloid lineages, and to a reduction of primitive hematopoietic progenitors, suggesting that the level of HOXB4 expression may determine the fate options following gene transfer. (dissertations.se)
After the in vitro differentiation of these embryonic stem cells to embryoid bodies, developing mesodermal progenitors could be separated from those with neuroectoderm potential based on GFP expression. (elsevierpure.com)
IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. (biolegend.com)

Transduction of Lhx2 into ESC-derived mesodermal cells resulted in robust differentiation of c-Kit+/Sca-1+/Lineage- (KSL) cells in vitro. (ebiotrade.com)
Furthermore, transplantation of Lhx2-transduced hematopoietic cells into lethally irradiated mice resulted in multi-lineage repopulation of hematopoietic cells over 4 months. (ebiotrade.com)
Gene-corrected Brca2Î"27/Î"27 iPSCs could be differentiated in vitro toward the hematopoietic lineage, although with a more limited efficacy than WT iPSCs or mouse embryonic stem cells, and did not engraft in irradiated Brca2Î"27/Î"27 recipients. (ibecbarcelona.eu)
To further understand the epigenetic regulators for specific lineage differentiation from iPS cell would have great significance for potential regeneration therapy and human disease modeling [ 8 ]. (biomedcentral.com)
This population of small cells includes a CD45-negative fraction that lacks hematopoietic stem cell and lineage markers and resides in the renal interstitial space. (tau.ac.il)
Heterochromatin exists in small regions (nucleation sites) in stem cells (for example, embryonic or hematopoietic stem cells) that expand differentially depending on the lineage track. (ucsf.edu)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)

During embryonic development, hematopoiesis occurs through primitive and definitive waves, giving rise to distinct blood lineages. (lu.se)
We demonstrate that glycolysis-mediated differentiation of HE toward primitive erythroid hematopoiesis is dependent on the epigenetic regulator LSD1. (lu.se)
In contrast, OXPHOS-mediated differentiation of HE toward definitive hematopoiesis is dependent on cholesterol metabolism. (lu.se)
We portrayed the Notch system in embryonic stem cell (ESC)-derived embryoid bodies (EBs) differentiating under the standard protocols used to assess yolk sac (YS) hematopoiesis in vitro. (karger.com)
Myelodysplastic syndrome (MDS) in childhood encompasses a diverse group of bone marrow disorders that share a common clonal defect of stem cells and that result in ineffective hematopoiesis with dysplastic changes in the marrow. (medscape.com)

Here, we show that the emergence of blood is regulated by multiple metabolic pathways that induce or modulate the differentiation toward specific hematopoietic lineages during human EHT. (lu.se)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)

abstract = "Adoptive cell transfer (ACT) of antigen-specific CD8+ cytotoxic T lymphocytes (CTLs) is a promising treatment for a variety of malignancies 1. (psu.edu)

Combining in vivo and in vitro assays, we show that the proliferation and differentiation capabilities of NSPCs decrease significantly in Ythdf2 −/− embryos. (biomedcentral.com)
This study aims to characterize the role of biomechanical modulation of the hemogenic vascular niche in HSC formation in vivo and in vitro, with the overall goal of identifying the signaling pathway(s) connecting select biophysical forces to the gene regulatory network controlling HSC commitment. (grantome.com)
For in vivo and in vitro studies, we employ next-generation sequencing and bioinformatic approaches to investigate underlying mechanisms. (hirschilabuva.com)

Karyotype analyses and comparative genome hybridization arrays of complemented Brca2Î"27/Î"27 iPSCs showed, however, the presence of different genetic alterations in these cells, most of which were not evident in their parental Brca2 Î"27/Î"27 mouse embryonic fibroblasts. (ibecbarcelona.eu)
Mouse embryonic fibroblasts isolated from transgenic Oct4-GFP reporter mice with or without Sirt6 were used for reprogramming by Yamanaka factors. (biomedcentral.com)
Cells usually consist of mouse embryonic fibroblasts. (cellmedicine.com)

Transduction of Lhx2 into induced pluripotent stem cells (iPSCs) was also effective in generating KSL cells in vitro, as well as HSC-like activities in vivo. (ebiotrade.com)
Gene complementation facilitated the generation of Brca2Î"27/Î"27 induced pluripotent stem cells (iPSCs) with a disease-free FA phenotype. (ibecbarcelona.eu)
Finally, based on analysis of the phenotype, genetic stability, and hematopoietic differentiation potential of gene-corrected Brca2Î"27/Î"27 iPSCs, achievements and limitations in the application of current reprogramming approaches in hematopoietic stem cell therapy are also discussed. (ibecbarcelona.eu)
Mouse somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc. (biomedcentral.com)
These new types of cells are called induced pluripotent stem cells (iPSCs). (healthline.com)
To create iPSCs, scientists genetically reprogram the adult stem cells so they behave like embryonic stem cells. (healthline.com)
They can be mainly classified into embryonic stem cells, adult stem cells and induced pluripotent stem cells (iPSCs) according to the basis of their resources. (creative-bioarray.com)
Considerable effort has thus been devoted to generating induced pluripotent stem cells (iPSCs) from multiple non-human primate species. (stanford.edu)
Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre-implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. (stanford.edu)
In summary, we disclose transcriptomic and proteomic data, cell lines, and cell culture resources that may be broadly enabling for non-human primate iPSCs research. (stanford.edu)

Furthermore, we showed that Sirt6-null iPS-like cell line has intrinsically a differentiation defect even though the establishment of normal self-renewal. (biomedcentral.com)
Stem cells are undifferentiated cells with the unique ability to develop into specialized cell types as the body needs them, and an enormous capacity for self-renewal to maintain the undifferentiated state. (creative-bioarray.com)
Cell growth, self-renewal ability and differentiation potential will also be fully characterized. (creative-bioarray.com)
Stem cells are defined by their capacity for self-renewal and multilineage differentiation, making them uniquely situated to treat a broad spectrum of human diseases. (articlecity.com)
Long-term self-renewal -The ability of stem cells to renew themselves by dividing into the same non-specialized cell type over long periods (many months to years) depending on the specific type of stem cell. (cellmedicine.com)

The results showed that the space microgravity environment provided favorable conditions for the 3D growth and maintenance of stemness of mouse embryonic stem cells, and stem cells cultured in space showed a better 3D growth mode than on the ground and maintained a higher level of pluripotent gene expression. (pdnews.cn)

Enforced expression of HOXB4 dramatically increases the regeneration of murine HSC following transplantation, and enhances the repopulating ability of human SCID repopulating cells. (dissertations.se)
The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
For example, because hematopoietic stem cells can reconstitute the entire blood system, bone marrow transplantation has long been used in the clinic to treat various diseases. (articlecity.com)
Similarly, the transplantation of other tissue-specific stem cells, such as stem cells isolated from epithelial and neural tissues, can treat mouse disease models and human patients in which epithelial and neural cells are damaged. (articlecity.com)
MDS is rare in childhood and may have a rapidly progressive course with an extremely poor prognosis without hematopoietic stem cell transplantation (HSCT). (medscape.com)

In 2017, the research team used the Tianzhou-1 cargo spacecraft to conduct research on the proliferation and differentiation of embryonic stem cells on a mouse. (pdnews.cn)
Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)

In bcjihkhm of the part djeca climate and action of ES strategies as students for both the example of bare story and new inappropriate assays, I moved it shareable to keep this author of otud debates( Embryonic Stem Cells) in which trojan manuscripts would contact technical sets for thechess of ES cross-species to make suitable vessels and framework ratios. (wk99.de)
This IL-5 protein is biologically active, and can be used for in vitro assays. (biolegend.com)

Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)
Adult stem cells have a misleading name, because they are also found in infants and children. (healthline.com)
Scientists have recently discovered how to turn adult stem cells into pluripotent stem cells. (healthline.com)
Adult stem cells don't present any ethical problems. (healthline.com)
Bone marrow-derived stem cells (BMSCs) are widely used as adult stem cells, which originate from the mesoderm [ 5 , 6 ]. (aging-us.com)
We have successfully isolated multiple types of stem cells from different resources, including embryonic stem cells, adult stem cells, iPSC, and tumor stem cells. (creative-bioarray.com)
Adult stem cells, which are present in small amounts in adult tissue but less adaptable than embryonic stem cells, making their use in medical treatments more challenging. (articlecity.com)
1,2 Regarding the origin, they can be classified as embryonic stem cells (ESCs) and adult stem cells (ASCs). (bvsalud.org)

In the process of blood vessel differentiation, the function of the protein ETS variant 2 (ETV2), which determines the differentiation into vascular endothelium, was first induced within 6 hours of differentiation stimulation. (medicalxpress.com)

P19 cells is an embryonic carcinoma cell line derived from an embryo-derived teratocarcinoma in mice. (wikipedia.org)
During the process of harvesting embryotic stem cells, the embryo is destroyed. (healthline.com)
In the developing embryo, TFs regulating the assembly/disassembly of transcriptional complexes and ultimately gene expression, are directed by complex extrinsic signalling processes which connect all cells within a multi-cellular organism to their environment. (amegroups.org)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
Blastocyst -A preimplantation embryo of about 150 cells. (cellmedicine.com)
Embryonic germ cells -Cells found in a specific part of the embryo/fetus called the gonadal ridge that normally develop into mature gametes. (cellmedicine.com)
Embryonic stem cells -Primitive (undifferentiated) cells from the embryo that have the potential to become a wide variety of specialized cell types. (cellmedicine.com)
These cells give rise to the embryonic disk of the later embryo and, ultimately, the fetus. (cellmedicine.com)
3,4 The zygote and cells derived from the first two cellular divisions constitute the most primitive cells (totipotent cells) that are capable of forming the embryo and the embryonic annexes (e.g. placenta, amniotic membranes etc). (bvsalud.org)

Currently, we lack markers that we can use to pinpoint the developmental stage of human ES cell derived candidate hematopoietic stem cells (HSC), and whether they have established the correct molecular machinery required for proper function. (ca.gov)
Embryonic blood cell development occurs via well-defined developmental stages which are recapitulated in vitro by differentiation of embryonic stem cells. (nih.gov)
To investigate the developmental processes that regulate mesoderm induction and specification to the hemangioblast, we generated an embryonic stem cell line with the green fluorescent protein (GFP) targeted to the mesodermal gene, brachyury. (elsevierpure.com)
Co-expression of GFP with the receptor tyrosine kinase Flk1 revealed the emergence of three distinct cell populations, GFP - Flk1 - , GFP + Flk1 - and GFP + Flk1 + cells, which represent a developmental progression ranging from pre-mesoderm to prehemangioblast mesoderm to the hemangioblast. (elsevierpure.com)
Stem cells have a very broad application prospect in the field of life sciences, such as developmental biology, cell biology, pathology and pharmacology. (creative-bioarray.com)
Directed differentiation of pluripotent stem cells: from developmental biology to therapeutic applications. (creative-bioarray.com)
However, all developmental processes originate from pluripotent precursor cell types. (amegroups.org)
We also highlight recent studies identifying the cis -regulatory elements such as enhancers at the global level and explain how their developmental activity is regulated by the cooperation of cell-type specific and ubiquitous transcription factors with extrinsic signals. (amegroups.org)
We show that the conditional depletion of the m 6 A reader protein Ythdf2 in mice causes lethality at late embryonic developmental stages, with embryos characterized by compromised neural development. (biomedcentral.com)
Research in the Hirschi Lab aims to uncover the developmental mechanisms of hemogenic endothelial cell specification and endothelial-to-hematopoietic transition, and apply insights gained to the generation of human HSPCs for clinical therapies. (hirschilabuva.com)
Frontiers in cell and developmental biology. (lu.se)

However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)
We performed a comprehensive screen for these TE exaptation events in 33 TCGA tumor types, 30 GTEx adult tissues and 675 cancer cell lines, and identified 1,068 TE-exapted candidates with the potential to generate shared tumor-specific TE-chimeric antigens (TS-TEAs). (bvsalud.org)
Cells Tissues Organs (2011) 193 (4): 239-252. (karger.com)
These stem cells come from developed organs and tissues in the body. (healthline.com)
Herein, we aimed to demonstrate the expression of miR-146a and FLAP in human HCC tissues and liver cancer cell lines. (biomedcentral.com)
We demonstrated that miR-146a expression is overexpressed, while FLAP protein and mRNA are suppressed in hepatocellular carcinoma tissues and HepG2 cells compared to para-carcinoma tissues and HL-7702 cells. (biomedcentral.com)
Creative Bioarray has extensive experience in isolating and culturing various kinds of stem cells from different tissues. (creative-bioarray.com)
Stem cells can be used to replace damaged cells and tissues. (creative-bioarray.com)
Long-term physioxic expansion also afforded culture-based ex vivo HSC selection from whole bone marrow, spleen, and embryonic tissues. (stanford.edu)
However, the accurate quantitation of mixed cell types in chimeric and mosaic tissues is complicated by sample preparation bias, transgenic silencing, phenotypic similarity, and low-throughput analytical pipelines. (stanford.edu)
Astrocyte -One of the large neuroglia cells of neural tissues. (cellmedicine.com)
Vascular perfusion is required for all tissues in the body to provide nutrients and oxygen, remove waste products, maintain fluid homeostasis, and traffic immune cells. (hirschilabuva.com)
In human postnatal dental tissues, five main sources of DSCs have been identified: dental pulp stem cells (DPSCs), 11 stem cells from human exfoliated deciduous teeth (SHEDs), 12 periodontal ligament stem cells (PDLSCs), 13 dental follicle stem cells (DFSCs) 14 and stem cells from apical papilla (SCAPs). (bvsalud.org)

In P19 cells, addition of non-toxic concentrations of drugs to aggregated embryoid body cells can induce differentiation of P19 cells into specific cell lines depending on the added drug. (wikipedia.org)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Directed differentiation -Manipulating stem cell culture conditions to induce differentiation into a particular cell type. (cellmedicine.com)

Here, we show that HSC-like cells were efficiently induced from mouse ESCs by enforced expression of Lhx2, a LIM-homeobox transcription factor. (ebiotrade.com)
However, the approach to obtain embryonic stem cells (ESCs) from patients is not feasible. (psu.edu)
3 Embrionic stem cells (ESCs) are derived from the inner cell mass of the blastocyst and form all cell types, derived from the three germ layers, and are therefore pluripotent. (bvsalud.org)
The use of ASCs in regenerative medicine and tissue engineering research has important advantages in comparison with ESCs, since there are no ethical complications and the process of differentiation of these cells is better controlled. (bvsalud.org)

Cell cultures containing undifferentiated stem cells were isolated from the primary tumor which have a euploid karyotype. (wikipedia.org)
CTLs can recognize malignant cells by interacting tumor antigens with the T cell receptors (TCR), and release cytotoxins as well as cytokines to kill malignant cells. (psu.edu)
Stimulation in vitro with a Notch ligand drives T cell differentiation from iPS cells, and TCR gene transduction results in iPS cells differentiating into antigen-specific T cells in vivo, which prevents tumor growth. (psu.edu)
In addition, we highlight tumor-specific membrane proteins transcribed from TE promoters that constitute aberrant epitopes on the extracellular surface of cancer cells. (bvsalud.org)
Sirt1, the mouse homologue of yeast Sir2 deacetylates several non-histone proteins and plays roles in many key functions, including energy metabolism, differentiation, aging, and tumor suppression [ 10 ]. (biomedcentral.com)
Abnormal expression of 5-Lipoxygenase Activating Protein (FLAP) has been detected in many tumor cells. (biomedcentral.com)
Accumulating evidence shows that cancer stem cells are key drivers of tumor formation, progression, and recurrence. (hindawi.com)
However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
Typically, rapidly proliferating tumor cells have glycolytic rates up to 200 times higher than those of their normal tissue of origin, even in the presence of oxygen [ 3 ]. (hindawi.com)

The clinical application of MSCs to tissue engineering has been introduced due to their numerous advantages including high expansion potential and extensive differentiation potential [ 6 , 7 ]. (hindawi.com)
In recent years, foreign scientists have also reported several times on research regarding stem cell growth and tissue regeneration carried out during space flight missions, such as blood stem cells for astronauts with anemia. (pdnews.cn)
Experts said that the unique space microgravity environment may be a new way to maintain undifferentiated proliferation of stem cells, enhance the efficiency of induced differentiation, and improve the level of three-dimensional tissue construction. (pdnews.cn)
Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
This means they can potentially produce new cells for any organ or tissue. (healthline.com)
Tissue engineering and cell therapy approaches aim to take advantage of the repopulating ability and plasticity of multipotent stem cells to regenerate lost or diseased tissue. (tau.ac.il)
However, MSCs have gained considerable attention for tissue regeneration owing to their differentiation ability with immunomodulatory effects. (techscience.com)
Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
They have the potential to be seed cells for cell therapy and tissue replacement therapy. (creative-bioarray.com)
and 2) bioreactor-based directed differentiation of ES cells under perfused 3D tissue-density conditions. (grantome.com)
With this raw material, biomedical firms can create stem cell lines that, among other things, aid recuperation via the regeneration of tissue that has been lost or damaged. (articlecity.com)
Current research is focused on growing a wide range of new tissue from stem cells, including muscle, blood, brain, and cartilage cells. (articlecity.com)
They have many of the positive characteristics of embryonic stem cells while sourcing material is far less challenging, since postpartum tissue can be used. (articlecity.com)
Bone marrow stromal cells -A stem cell found in bone marrow that generates bone, cartilage, fat, and fibrous connective tissue. (cellmedicine.com)
The lab also uses human stem cell culture models to study the regulation of human endothelial cell differentiation and specification, and to study the functional roles of endothelial cells in regulating tissue-resident stem cells. (hirschilabuva.com)
In addi tion, LNCaP C4 2B cells express a promiscuous kind in the androgen receptor, possessing the most AR frequent sub stitution, which can be repeatedly observed in prostate cancer selleck chemical tissue specimens of individuals with AIPC. (hdac-inhibitors.com)
The relevance of these phenomena in stem cell biology and tissue repair is discussed. (unicyte.ch)
Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)
of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)
In explant method, the dental tissue is placed on a plastic surface and the cells migrate out from the tissue fragment adhering to culture flasks or dishes ( Figure 1 ). (bvsalud.org)
Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)

Embryoid bodies -Clumps of cellular structures that arise when embryonic stem cells are cultured. (cellmedicine.com)

In both in vitro and in vivo settings, steering pyruvate use toward glycolysis or OXPHOS differentially skews the hematopoietic output of HE cells toward either an erythroid fate with primitive phenotype, or a definitive lymphoid fate, respectively. (lu.se)
Heterochromatin spreading plays a key role in the genome partitioning process that shapes cell fate. (ucsf.edu)
The stimulus for the change of cell fate originates from extrinsic signals which set a cascade of intracellular processes in motion that eventually terminate at the genome leading to changes in gene expression and the development of alternate gene regulatory networks. (amegroups.org)
While mechanical properties of the niche, including sheer stress and circumferential stretch, are increasingly recognized as important stem cell cues in many contexts, the mechanism(s) by which mechanotransduction drives commitment to hemogenic fate and HSC productionduring vertebrate development remain largely unexplored. (grantome.com)
Endothelial cell specialization is associated with suppression of cell growth, but how signaling pathways that control endothelial cell cycle and fate are coordinated was not known. (hirschilabuva.com)
In subsequent studies using the Fluorescent Ubiquitination Cell Cycle Indicator (FUCCI) reporter, we found that endothelial cell cycle state determines propensity for arterial-venous fate. (hirschilabuva.com)
Endothelial Cell Cycle State Determines Propensity for Arterial-Venous Fate. (hirschilabuva.com)
The effectiveness of stem cell therapies has been hampered by cell death and limited control over fate. (nature.com)
Harnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate. (nature.com)
Khetan, S. & Burdick, J. A. Patterning network structure to spatially control cellular remodeling and stem cell fate within 3-dimensional hydrogels. (nature.com)
The fate of the cell is deter- mined by coordinated and dynamic interactions among a number of factors, acting in a defined microenviron- ment. (unicyte.ch)
In normal cells, the fate of pyruvate depends on many factors, one of which is oxygen availability. (hindawi.com)

Because the concentration of retinoic acid or DMSO is nontoxic to the cells, the drug-specific differentiation is due to induction of cells not selection. (wikipedia.org)
The KSL cell induction frequency was superior to the case of Hoxb4. (ebiotrade.com)
In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system. (biomedcentral.com)
The c-kit + cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. (biomedcentral.com)
Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)

Differentiation -The process whereby an unspecialized early embryonic cell acquires the features of a specialized cell such as a heart, liver, or muscle cell. (cellmedicine.com)

Therefore, it is particularly important to clarify the molecular and regulatory mechanisms of differentiation of bone marrow-derived stem cells (BMSCs) into liver cells. (aging-us.com)

The majority of ES clones that expressed lacZ in blood islands also expressed lacZ upon differentiation into hematopoietic cells on OP9 stromal layers. (ox.ac.uk)

These lines will serve as tools to identify factor combinations to improve the in vitro differentiation of hESCs to functional blood stem cells. (ca.gov)
Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)
Embryonic stem cell-specific miR302-367 cluster: human gene structure and functional characterization of its core promoter. (research.com)
We have developed a large-scale, expression-based gene trap strategy to perform genome-wide functional analysis of the murine hematopoietic and vascular systems. (ox.ac.uk)
Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
In the present review we focus on the extracellular vesicle-induced epigenetic changes in recipient cells that may lead to phenotypic and functional modifications. (unicyte.ch)
Information exchange between cells coordinates devel- opment and functional interplay in complex organisms. (unicyte.ch)

However, due to difficulties in obtaining a high number of such CTLs from patients, there is an urgent need to find a new approach to generate highly reactive Ag-specific CTLs for successful ACT-based therapies.TCR transduction of the self-renewable stem cells for immune reconstitution has a therapeutic potential for the treatment of diseases 8-10 . (psu.edu)
Our goal is to develop tools that address major bottlenecks that have prevented the generation of blood forming stem cells in culture for therapeutic use. (ca.gov)
The results indicate that the lncRNA-miRNA-mRNA interaction chain may play an important role in the differentiation of BMSCs into hepatocytes, which provides a new therapeutic target for liver disease treatment. (aging-us.com)
Potential therapeutic roles of stem cells in ischemia-reperfusion injury. (creative-bioarray.com)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
Stem cell behaviour can also be controlled in vitro by manipulating the elasticity of both porous and non-porous materials, yet translation to therapeutic processes in vivo remains elusive. (nature.com)
Our findings show that biophysical cues can be harnessed to direct therapeutic stem cell behaviours in situ . (nature.com)

Once individual lines have been validated, lines that contain multiple fluorescent reporters will be generated, and a multi factor screen will be performed to optimize conditions that induce these blood stem cell regulators. (ca.gov)

Finally, when injected directly into the renal parenchyma, shortly after ischemic/reperfusion injury, renal Sca-1 + Lin - cells, derived from ROSA26 reporter mice, adopt a tubular phenotype and potentially could contribute to kidney repair. (tau.ac.il)
These data define a unique phenotype for adult kidney-derived cells, which have potential as stem cells and may contribute to the regeneration of injured kidneys. (tau.ac.il)
These problems can be partially circumvented by using macroporous biomaterials that improve the survival of transplanted stem cells and provide molecular cues to direct cell phenotype. (nature.com)
Growing evidence suggests that transcriptional regulators and secreted RNA molecules encapsulated within membrane vesicles modify the phenotype of target cells. (unicyte.ch)
Growth factors may act either in an autocrine or a paracrine fashion and their temporal and spatial concentration modulates the cell phenotype and function. (unicyte.ch)
The cell phenotype is therefore determined by signals that target the cells received within a defined microenvironment. (unicyte.ch)

Moreover, signaling pathways related to neurogenesis and myogenesis were also investigated by studying gene expression or generating mutants of P19 cells. (wikipedia.org)
First, we identified two NEPC gene expression signatures representing different phases of trans-differentiation. (bvsalud.org)
This complex process is regulated by the interplay of transcription factors with a chromatin environment, both of which provide the epigenetic information maintaining cell-type specific gene expression patterns. (amegroups.org)
Embryonic development is tightly controlled at the level of gene expression. (amegroups.org)
Gene expression The affect of DADS therapy on cytokine induced metalloproteinase gene expression was assessed from the SW1353 cell line by TaqMan qRT PCR. (hdac-inhibitors.com)

During the creation of new blood vessels, the vascular endothelial growth factor (VEGF) protein differentiates stem cells into vascular endothelial cells and stimulates them to create new blood vessels. (medicalxpress.com)
The protein GATA2, which binds to ETV2 and supports vascular endothelial differentiation, was induced immediately thereafter. (medicalxpress.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
Vascular endothelial cell specification in health and disease. (hirschilabuva.com)

However, researchers utilize these cells to study the development of cancer cells in order to find more specific treatments. (wikipedia.org)
Recent iPS cell technology and the development of an in vitro system for gene delivery are capable of generating iPS cells from patients without any surgical approach. (psu.edu)
Our goal is to generate a toolbox of human embryonic stem cell lines to monitor blood forming (hematopoietic) stem cell development in culture. (ca.gov)
To help overcome these bottlenecks, we will generate a suite of human embryonic stem cell reporter lines that can be used to monitor key milestones in blood stem cell development. (ca.gov)
These findings provide novel mechanistic insights into vascular and hematopoietic development. (nih.gov)
HOXB4 is one of 39 members of the homeobox (HOX) family, a family of transcription factors involved in embryonic development. (dissertations.se)
Notch receptors and Notch ligands were detected in virtually all cells throughout EB development. (karger.com)
This provides a solid foundation for the establishment of disease cell models, drug screening, pharmacological and toxicology research, and new drug development. (creative-bioarray.com)
The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Embryonic stem (ES) cell research and scale-up for development of possible clinical therapies is limited by the existing 2D dish culture methods. (grantome.com)
Embryonic stem cells, which must be extracted from embryos three to five days old (known as blastocysts, which contain only about 150 cells at this point of development). (articlecity.com)
Induced pluripotent stem cells, which harness the power of genetic reprogramming - basically, the altering of a cell's DNA - to change the course of cellular development. (articlecity.com)
The Hirschi Lab is broadly interested in endothelial cell differentiation, proliferation, specification and function during development. (hirschilabuva.com)
Endothelial Cell Development and Its Application to Regenerative Medicine. (hirschilabuva.com)
The development of LNCaP C4 2B cells in androgen no cost medium was appreciably reduced by therapy using the Hedgehog pathway inhibi tor cyclopamine, the EGFR inhibitor gefitinib and also the EGFR and ErbB2 inhibitor lapatinib. (hdac-inhibitors.com)
Calculation of your drug concentration creating the median impact of 50% development inhibi tion within the LNCaP C4 2B cell line in androgen absolutely free medium was performed through the dose response curves for each drug, and had been similar to these reported within the literature. (hdac-inhibitors.com)
A tight spatial and timing regulation of growth factor action during embryonic development has been suggested [4]. (unicyte.ch)
These cells have been studied for the treatment of periodontitis, bone repair, regeneration of the pulp after necrosis as well as the development of new teeth. (bvsalud.org)
Y14 is part of the exon-junction complex (EJC), a set of proteins associated with transcript export, localization, and splicing, playing a critical in embryonic development. (medscape.com)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)

They can be divided into totipotent stem cells capable of differentiation into all possible cell types, multipotent stem cells capable of differentiation into a closely related family of cells, pluripotent stem cells capable of differentiation into almost all cell types, oligopotent stem cells capable of differentiation into a few cells, and unipotent stem cells capable of producing only their own cell types. (creative-bioarray.com)

Membrane vesicles, actively released by cells, represent a mechanism of intercellular communication that is conserved evolutionarily and involves the transfer of molecules able to induce epigenetic changes in recipient cells. (unicyte.ch)
Similarly, reprogramming of somatic cells involves a complex interaction among intracellular and extracellular signals leading to epigenetic remodeling [6]. (unicyte.ch)

P19 cells can be maintained in exponential growth because of a stable chromosomal composition. (wikipedia.org)
Cellular senescence refers to active cells that eventually enter a state of irreversible growth arrest. (hindawi.com)
The research team will also continue to conduct research on the three-dimensional growth of induced pluripotent stem cells in the space environment, taking the opportunity presented by the Tianzhou-7 or Tianzhou-8 cargo spacecraft. (pdnews.cn)
Although Myo52 has been shown to have roles in vacuole distribution, cytokinesis and cell growth, Myo51 has no as yet discernible function in the vegetative life cycle. (openrepository.com)
Hematopoietic Stem Cell and Leukaemic Stem Cell environmental stress responses (In vitro biomimicry of hypoxia, normoxia, hypothermia, hyperthermia, hypoglycemia and hyperglycemia): Effect on cellular growth, cell cycle distribution, apoptosis and cellular metabolism. (imperial.ac.uk)
Cell-to-cell signalling is induced by specific ligands such as growth factors which activate their cognate receptor molecules. (amegroups.org)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
Cell culture -Growth of cells in vitro on an artificial medium for experimental research. (cellmedicine.com)
Culture medium -The broth that covers cells in a culture dish, which contains nutrients to feed the cells as well as other growth factors that may be added to direct desired changes in the cells. (cellmedicine.com)
Microenvironment -The molecules and compounds such as nutrients and growth factors in the fluid surrounding a cell in an organism or in the laboratory, which are important in determining the characteristicsof the cell. (cellmedicine.com)
These cells were initially isolated and characterised following growth in castrated athymic mice of androgen http://www.selleckchem.com/products/arq-197.html dependent LNCaP prostate cancer cells from your web site of bony metastasis. (hdac-inhibitors.com)
Importantly, the growth of LNCaP C4 2B cells is just not impacted by withdrawal of androgens, confirming the androgen independence of these cells and these cells express androgen receptor and PSA. (hdac-inhibitors.com)
To determine the importance of the Hedgehog and ErbB pathways to AIPC cell growth we handled LNCaP C4 2B cells with unique inhibitors to cyclopamine which blocks Hedgehog signalling, gefitinib and lapatinib, both singularly or in combination. (hdac-inhibitors.com)

After 10 days of exposure, astroglial cells can be detected using glial fibrillary acidic protein (GFAP), which is a specific marker of glial cells. (wikipedia.org)
It has been reported that Sirtuin 6 (Sirt6), a member of the sirtuin family of NAD + -dependent protein deacetylases, is involved in embryonic stem cell differentiation. (biomedcentral.com)
IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. (biolegend.com)

Cellular senescence, which is morphologically characterized by an enlarged and flattened cell shape, was first described by Hayflick [ 9 ]. (hindawi.com)
Additionally, the interwoven fibers provide a scaffold allowing the cells to form 3D structures where the size of cellular aggregates is limited by the spacing between the hollow fibers. (grantome.com)
The proposed research will elucidate how biomechanical forces resulting from the onset of embryonic circulation cue the cellular commitment and vascular remodeling necessary for HSC formation via Rho-Yap activation. (grantome.com)
Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
In normal cells, glucose participates in cellular energy production through glycolysis as well as through its complete catabolism via the tricarboxylic acid (TCA) cycle and oxidative phosphorylation (OXPHOS). (hindawi.com)
not unique for the embryonic system but are shared by other ogenesis regulation must consider different scales of multi-cellular modeling areas. (lu.se)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)

We previously identified binding motifs for the commonly expressed AP-1 transcription factor family in open chromatin regions specific for early stages of blood specification and thus aimed to study the role of AP-1 for hemangioblast differentiation. (nih.gov)
Dynamically and epigenetically coordinated gata/ets/sox transcription factor expression is indispensable for endothelial cell differentiation. (medicalxpress.com)
Furthermore, they found that a transcription factor group essential for blood vessel differentiation (ETS/GATA/SOX) has a previously unknown role. (medicalxpress.com)
Schematic representation of the genetic regulatory network linking four transcription factors and EC differentiation. (medicalxpress.com)
Transcription factors SOX and FLI1, both important for endothelial differentiation, were induced between 12 and 24 hours. (medicalxpress.com)
Previously, in the region that controls the function of the transcription factor that promotes differentiation from ES cells to a specific cell type, bivalent modifications of histones such as the accelerator and brake histone marks for transcription were thought to have coexisted. (medicalxpress.com)
Despite more than a decade of research, current protocols rely primarily on enforced expression of transcription factors to help steer cells into an ?HSC-like? (grantome.com)
In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
Ident- cult to deduce interaction rules from observations of the ified transcription factors for specific cell fates are, for entire system. (lu.se)

Our in vivo tracking tools will be broadly relevant and not restricted to studying the in vivo biology of blood forming cells. (ca.gov)
Over the past few decades, in addition to advances in biological treatment research, molecular biology, cell bioengineering, and the stem cell research, stem cell therapy has emerged as an economic and feasible liver disease treatment for the end-stage liver disease [ 3 ], particularly decompensated cirrhosis liver failure and advanced liver cancer, and offers an effective strategy with no limit of supply and demand [ 3 ]. (aging-us.com)
MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of The Digital Cell: Cell Biology as a Data Science, available on its website in hardcover format. (cshlpress.com)

Methylation state on the histone tail on the chromatin, histone code, in endothelial cells was read via hi-speed deep sequencer. (medicalxpress.com)
MiR-146a has also been shown to promote the angiogenic activity of endothelial cells in HCC patients [ 11 ]. (biomedcentral.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)

This can provide more beneficial assistance for the future use of stem cell regeneration to serve human health. (pdnews.cn)
and stem cell regeneration. (lu.se)

Widespread Notch1 activation was evident at days 2-4 of EB differentiation, the time window of hemangioblast generation in this in vitro system. (karger.com)

Decreased proliferation and Nanog expression in mouse embryonic stem (ES) cells, with increased erythroid differentiation (Chae et al. (stemcell.com)

Among them is the first international research on human pluripotent stem cells in vitro hematopoietic differentiation of human embryonic stem cells under space conditions. (pdnews.cn)
From the experimental results, China has successfully achieved the first hematopoietic differentiation of human embryonic stem cells under space conditions, accomplishing its first experimental goal. (pdnews.cn)
These tools will help translate the promise of stem cells to cell based therapies to treat human disease. (ca.gov)
Embryonic stem cells come from human embryos that are three to five days old. (healthline.com)
However, in recent years, there has been controversy surrounding the way human embryonic stem cells are obtained. (healthline.com)
Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells. (research.com)
Since human induced pluripotent stem cell (iPSC)-platelet products can be supplied independently from the donor, it is expected to complement current platelet products. (biomedcentral.com)
This is because stem cells are, basically, the core building blocks of all human cells - which is why they are most prominently a part of human physiology during gestation in the womb. (articlecity.com)
Stem cells are unspecialized cells of the human body. (articlecity.com)
Human embryonic stem cell -A type of pluripotent stem cell derived from the inner cell mass of the blastocyst. (cellmedicine.com)
Retinoic Acid Promotes Endothelial Cell Cycle Early G1 State to Enable Human Hemogenic Endothelial Cell Specification. (hirschilabuva.com)
8,9 Because dental stem cells (DSCs) are easy to obtain and present a great potential of differentiation, there has been a growing interest in their use in regenerative medicine for treatment of various human diseases. (bvsalud.org)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
Here we describe how this system can be used for human embry- onic stem cells. (lu.se)

Studies in our knockout mice reveal that deficiency of HOXB4 leads to a hypocellularity in hematopoietic organs and impaired proliferative capacity. (dissertations.se)

To track the location and quantity of transplanted cells in recipient small animal model, we will generate hESC lines with in vivo reporter system that combines bioluminescent or PET imaging, and serum-based assay. (ca.gov)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)

Ongoing research is focused on understanding mechanisms of endothelial cell specification towards hemogenic, arterial, venous and lymphatic fates. (hirschilabuva.com)
Ongoing studies are further investigating underlying regulatory mechanisms, the role of endothelial cell cycle state in lymphatic specification, and the role of dysregulated of endothelial cell cycle state in vascular malformations and diseases. (hirschilabuva.com)
Shear-induced Notch-Cx37-p27 axis arrests endothelial cell cycle to enable arterial specification. (hirschilabuva.com)
Regulation of endothelial cell differentiation and specification. (hirschilabuva.com)

Pablo Menendez performs multidisciplinary study in Embryonic stem cell and Induced pluripotent stem cell in his work. (research.com)
Pluripotent stem cell sources and their application. (creative-bioarray.com)

Anatomy59

Organisms13

Diseases8

Chemicals and Drugs66

Analytical, Diagnostic and Therapeutic Techniques and Equipment49

Phenomena and Processes62

Disciplines and Occupations5

Technology, Industry, Agriculture3

Information Science3

HSCs20

Progenitor11

Genes22

Differentiate21

Mesenchymal13

Progenitors3

Lineage7

Hematopoiesis5

Lineages2

Abstract1

Vivo and in vitro3

Fibroblasts3

IPSCs10

Self-renewal5

Mouse embryonic stem1

Transplantation7

Proliferation and differentiation2

Assays2

Adult stem8

Endothelium1

Embryo9

Developmental11

Tissues13

Induce differentiation3

ESCs4

Tumor9

Tissue21

Embryoid Bodies1

Fate12

Induction7

Early embryonic1

Bone marrow-derived1

Upon differentiation1

Functional8

Therapeutic7

Conditions that induce1

Phenotype6

Gene expression5

Vascular endothelial4

Development22

Multipotent1

Epigenetic2

Growth14

Protein3

Cellular7

Transcription9

Biology4

Endothelial cells3

Regeneration2

Hemangioblast1

Erythroid1

Human16

Proliferative capacity1

Reporter2

Specification4

Pluripotent stem2