Hematopoietic stem cells structural clone cells. Medical search. Frequent questions

and regulation of stem cell differentiation. (nih.gov)
The modification has been shown to be critical to cell differentiation, animal development, and a range of biological signaling and stress response. (chicagobiomedicalconsortium.org)
Cellular RNA methylation level can have profound impacts on normal cell differentiation and cancer cell proliferation. (chicagobiomedicalconsortium.org)
Several genes involved in different aspects of stem cell activation are being studied, including some that transduce initial signals, and others that regulate self-renewal and differentiation. (chicagobiomedicalconsortium.org)
Sequential rounds of division and differentiation give rise to an enormous amplification of cell numbers, necessary for the production of mature blood cells. (justia.com)
This process of division and differentiation is subject to regulation at many levels to control cell production. (justia.com)
DNMT3A is a critical regulator of hematopoietic stem cell (HSC) differentiation and somatic DNMT3A mutations are frequent in hematologic malignancies and clonal hematopoiesis. (haematologica.org)
We have used RT-PCR to screen pluripotent murine embryonic stem cells to identify receptor tyrosine kinases (RTKs) potentially involved in the determination or differentiation of cell lineages during early mouse development. (silverchair.com)
Myelophthisis is a form of bone marrow failure that results from the destruction of bone marrow precursor cells and their stroma, which nurture these cells to maturation and differentiation. (medscape.com)
Differentiation -The process whereby an unspecialized early embryonic cell acquires the features of a specialized cell such as a heart, liver, or muscle cell. (cellmedicine.com)
Directed differentiation -Manipulating stem cell culture conditions to induce differentiation into a particular cell type. (cellmedicine.com)
Embryonic stem cell line -Embryonic stem cells, which have been cultured under in vitro conditions that allow proliferation without differentiation for months to years. (cellmedicine.com)
Sphingosine-1-Phosphate Receptor 3 Potentiates Inflammatory Programs in Normal and Leukemia Stem Cells to Promote Differentiation. (jdstemcellresearch.ca)
These signatures inversely correlated during early hematopoietic commitment and differentiation. (jdstemcellresearch.ca)
In order to better understand the factors that regulate B cell differentiation upon exposure to antigen, we compares global gene expression profiles from naive B cells with antigen-specific plasma, germinal center, and memory B cells after immunization with the T-dependent antigen, NP-CGG. (omicsdi.org)
This was correlated with the appearance of CGG-specific B cells and their growth and differentiation to plasma cells. (omicsdi.org)
The same pattern was seen for the migration, growth, and differentiation of virgin hapten-specific B cells when CGG-primed mice were challenged with hapten protein. (omicsdi.org)
Ideally, iPSC-based therapies in the future will rely on the isolation of skin fibroblasts or keratinocytes, their reprogramming into iPSCs, and the correction of the genetic defect followed by differentiation into the desired cell type and transplantation. (biomedcentral.com)
As immune cells typically undergo differentiation from precursor or naïve cells to mature cells, it is possible to observe the cell differentiation process by using iPS cell-derived immune cells. (biomedcentral.com)
The data concerning genetic and protein markers of thyroid stem cells, as well as markers of their thyroid differentiation, are systematized. (genescells.ru)
Physiological and pathological regulation of thyroid cell proliferation and differentiation by thyrotropin and other factors. (genescells.ru)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

Therefore, a central goal in clinical care of SDS patients is to identify incipient leukemic transformation and initiate pre-emptive treatment with allogeneic stem cell transplantation. (nature.com)
Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
Edward Donnall Thomas, an American physician and scientist, gained recognition in the scientific community for conducting the first bone marrow transplant, a pioneering form of hematopoietic stem cell transplantation (HSCT). (asu.edu)
Peripheral blood stem cell mobilization is very important in hematopoietic stem cell transplantation (as a recent alternative to transplantation of surgically harvested bone marrow ) and is currently performed using drugs such as G-CSF . (wikidoc.org)
Hematopoietic stem cell transplantation (bone marrow, cord blood, or peripheral blood stem cells) may cure aplastic anemia and prevent myelodysplastic syndrome or leukemia. (medscape.com)
In addition, as the genetic identity of the donor egg from which the ESCs are derived most likely will differ from that of potential recipients, patients who receive ESC-derived cells or tissues may face the same complications that result from organ transplantation (for example, immunorejection, graft-versus-host disease, and need for immunosuppression). (biomedcentral.com)
Consolidative allogeneic hematopoietic stem cell transplantation after chimeric antigen receptor T-cell therapy for relapsed/refractory B-cell acute lymphoblastic leukemia: who? (biomedcentral.com)
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) after CAR T-cell therapy has emerged as a promising strategy to prolong LFS. (biomedcentral.com)
For decades, allogeneic hematopoietic stem cell transplantation (allo-HSCT) has been regarded as the only well-established curative cellular therapy for patients with B-ALL. (biomedcentral.com)

The lab is investigating the role of DNA methyltransferase 3a (DNMT3A) in regulating stem cell function, and how mutations in DNMT3A lead to age-related clonal hematopoiesis and development of hematologic malignancies. (chicagobiomedicalconsortium.org)
Goodell reported that PPM1D mutations confer a survival advantage onto hematopoietic clones by rendering them resistant to DNA-damaging agents such as cisplatin, a cytotoxic chemotherapeutic. (chicagobiomedicalconsortium.org)
Here we report that multiple independent somatic hematopoietic clones arise early in life, most commonly harboring heterozygous mutations in EIF6 or TP53 . (nature.com)
Somatic TP53 mutations have been observed in patients with SDS who develop MDS 13 , raising the possibility that next-generation sequencing could be integrated into surveillance for somatic clones with enhanced leukemia potential. (nature.com)
Myelodysplastic syndromes are a group of clonal hematopoietic stem cell disorders unified by the presence of distinct mutations of hematopoietic stem cells, most frequently in genes involved in RNA splicing. (msdmanuals.com)
Risk increases with age due to the acquisition of somatic mutations that can promote clonal expansion and dominance of a particular hematopoietic stem cell, and possibly due to exposure to environmental toxins such as benzene, radiation, and chemotherapeutic agents (particularly long or intense regimens and those involving alkylating agents, hydroxyurea , and/or topoisomerase inhibitors). (msdmanuals.com)
Somatic, inactivating PIGA mutations in haematopoietic stem cells, followed by an unknown autoimmune selection process in favor of the mutated clone, are thought to be important events in the pathogenesis of paroxysmal nocturnal hemoglobinuria (PNH). (annexpublishers.co)
have recently sequenced the exome of affected cells in 12 PNH patients, and found a complex pattern of clonal evolution denoted by shared and distinct mutations in subclones, but not a single recurrent mutation besides PIGA [ 19 ]. (annexpublishers.co)
[9] WHIM like mutations in CXCR4 were recently identified in patients with Waldenstrom's macroglobulinemia , a B-cell malignancy. (wikidoc.org)
Since autoinflammatory diseases are usually monogenic, genetic mutations affect the cell function and patient-derived iPS cells tend to exhibit disease-specific phenotypes. (biomedcentral.com)
However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
Aging is also associated with a higher prevalence of acquired somatic mutations, especially in frequently regenerating cells, such as hematopoietic stem cells (HSC). (biomedcentral.com)
Patients with relapsed/refractory B-cell acute lymphoblastic leukemia (r/r B-ALL) often have clinicopathological characteristics associated with poor prognosis, such as high tumor burden and high-risk gene mutations. (biomedcentral.com)

This fibrosis destroys the normal hematopoietic cells and their supportive stromal cells. (medscape.com)
The bone marrow becomes infiltrated by collagen, reticulin, and other forms of fibrosis, which replace the normal, hematopoietic cells. (medscape.com)

The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
The various mature blood cell types are all ultimately derived from a single class of progenitor cell known as hematopoietic stem cells. (justia.com)
As few transcriptional changes underlie this transition, we used single-cell and bulk assay for transposase-accessible chromatin sequencing (ATAC-seq) on human HSCs and hematopoietic stem and progenitor cell (HSPC) subsets to uncover chromatin accessibility signatures, one including LT-HSCs (LT/HSPC signature) and another excluding LT-HSCs (activated HSPC [Act/HSPC] signature). (jdstemcellresearch.ca)
We searched the databases such as EMBASE, PubMed, and Web of Science with the keywords "stem cell," "progenitor cell," "satellite," "senescence" and excluded the keywords "cancer," "tumor," "malignancy" and "carcinoma" until June 2020. (magiran.com)

Bone marrow stromal cells -A stem cell found in bone marrow that generates bone, cartilage, fat, and fibrous connective tissue. (cellmedicine.com)

Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. (rndsystems.com)
The subject's blood can be processed and used to repopulate the destroyed lymphohematopoietic system of a recipient and may in the future be utilized to repair a variety of non-hematopoietic tissues. (justia.com)
Researchers are uncovering ever more details about the complex system of organs, tissues, and different cell types that make up the human immune system. (mdc-berlin.de)
Astrocyte -One of the large neuroglia cells of neural tissues. (cellmedicine.com)
Cell-based therapies -treatment in which stem cells are induced to differentiate into the specific cell type required to repair damaged or depleted adult cell populations or tissues. (cellmedicine.com)
Expression of this receptor in cancer cells has been linked to metastasis to tissues containing a high concentration of CXCL12, such as lungs, liver and bone marrow. (wikidoc.org)
In human disease studies, ethical concerns often restrict the collection of patient cells and tissues. (biomedcentral.com)
Adherent cell layers derived from other haemopoietic organs (foetal liver, adult spleen) and fibroblasts from embryonic tissues did not maintain haemopoietic cells in this system. (tno.nl)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

Cells usually consist of mouse embryonic fibroblasts. (cellmedicine.com)
In 2006, a major breakthrough was reported in Japan by Takahashi and Yamanaka, who described the generation of induced pluripotent stem cells (iPSCs) from mouse fibroblasts via over expression of defined transcription factors [ 2 ]. (biomedcentral.com)
Particularly, non-hematopoietic cells, such as fibroblasts and cardiomyocytes, differentiated from autoimmune patient-derived iPS cells have shown promising results for further research into the pathogenesis. (biomedcentral.com)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

Biochemically, PNH is a consequence of nonmalignant clonal expansion of hematopoietic stem cells with somatic mutation of PIGA [ 2 , 3 ], and mutant, inactive PIGA explains the deficiency of glycosyl phosphatidylinositol-anchored proteins [ 4 ]. (annexpublishers.co)
The impressive early production of switch transcripts during T cell-dependent antibody responses occurs in cells that are about to undergo massive clonal expansion. (omicsdi.org)

Measured by its ability to inhibit Flt-3 Ligand-induced proliferation of BaF3 mouse pro‑B cells transfected with Flt-3. (rndsystems.com)
Recently, a second clonal event involving the HMGA2 gene was reported in some PNH cases, and over expression of this gene was postulated to promote proliferation of the mutated clone. (annexpublishers.co)
Chronic B cell activation may drive proliferation of antigen-selected B cell clones that accumulate genetic lesions and ultimately undergo neoplastic transformation. (biomedcentral.com)
Acute myeloid leukemia (AML) results from the over-proliferation of progenitor cells of the myeloid lineage in the bone marrow. (ghrnet.org)
In vitro proliferation of haemopoietic cells in the presence of adherent cell layers. (tno.nl)
The culture system, in which a marrow-derived adherent cell population, established in vitro, exerts a long-term promoting influence on proliferation of haemopoietic cells, is reproduced. (tno.nl)
Mouse bone marrow-derived adherent cell populations promoted proliferation of haemopoietic cells in vitro in a liquid culture system for at least 4 weeks. (tno.nl)
Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
This activation is necessary for both cell proliferation as well as glucose uptake and use. (hindawi.com)

Switchable targeting of solid tumors by BsCAR T cells. (yale.edu)
[14] Another explanation of this observation is provided by a study that shows the ability of CXCL12 (and CCL2 ) producing tumors to entrain neutrophils that inhibit seeding of tumor cells in the lung. (wikidoc.org)
Here we report a phenotypic and molecular characterization of B cell tumors that develop in Tg26 mice. (biomedcentral.com)
Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)

The heat in this discussion was first ignited in 2001, when President Bush limited federally funded embryonic stem cell research to stem cell lines that were already in existence. (pharmexec.com)
Since then, demand for access to embryonic stem cell lines has swelled, prompting universities such as Harvard, Stanford, Massachusetts Institute of Technology, and the Universities of Wisconsin and Minnesota to use private money to pursue research initiatives. (pharmexec.com)
Also in 2001, David Beck, PhD, president of the Camden, New Jersey-based Coriell Institute for Medical Research, which houses the largest repository of cell cultures in the world, made a prescient remark: 'Much of the controversy regarding embryonic stem-cell research will go away, because we're learning so much about stem cell biology. (pharmexec.com)
The problem is that these human embryonic stem cell lines have been grown in such a way that they are contaminated by animal protein and cannot be used for human therapeutic purposes,' Beck explains. (pharmexec.com)
In April 2004, 204 members of the House of Representatives, including 36 Republicans, signed a letter urging President Bush to rethink the restrictions on embryonic stem cell research, suggesting that hundreds of thousands of embryos stored in in-vitro fertilization (IVF) clinics could be used to advance embryonic stem cell research. (pharmexec.com)
Human embryonic stem cell -A type of pluripotent stem cell derived from the inner cell mass of the blastocyst. (cellmedicine.com)

The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
Here we identified distinct subsets of human long-term (LT)-HSCs that responded differently to regeneration-mediated stress: an immune checkpoint ligand CD112 lo subset that exhibited a transient engraftment restraint (termed latency) before contributing to hematopoietic reconstitution and a primed CD112 hi subset that responded rapidly. (jdstemcellresearch.ca)

True stem cells are both pluripotent-that is they can give rise to all cell types-and capable of self-renewal. (justia.com)
Shinya Yamanaka gained international prominence after publishing articles detailing the successful generation of induced pluripotent stem (iPS) cells, first in mice, then in humans. (asu.edu)
John D. Gearhart is a renowned American developmental geneticist best known for leading the Johns Hopkins University research team that first identified and isolated human pluripotent stem cells from human primordial germ cells, the precursors of fully differentiated germ cells. (asu.edu)
Feeder layer -Cells used in co-culture to maintain pluripotent stem cells. (cellmedicine.com)
Our investigation indicates that most of these studies examined endothelial progenitor cells, hematopoietic stem cells, mesenchymal stem cells, adipose-derived stem cells, and a few others were about less-discussed types of stem cells such as cardiac stem cells, myeloblasts, and induced pluripotent stem cells. (magiran.com)
Derivation of autologous induced pluripotent stem cells (iPSCs) through direct reprogramming of easily accessible somatic cells holds the potential to transform the field of regenerative medicine. (biomedcentral.com)
Here, we summarize current reprogramming methodologies with a focus on the production of transgene-free or genetically unmanipulated iPSCs and highlight important technical details that ultimately may influence the biological properties of pluripotent stem cells. (biomedcentral.com)
Although exciting results have been achieved by means of somatic cell nuclear transfer, cell fusion, and culture-induced reprogramming [ 1 ], these procedures are technically demanding and inefficient and therefore unlikely to become a common approach for producing patient-specific pluripotent cells. (biomedcentral.com)
Basic research utilizing immune-related disease patient-derived induced pluripotent stem (iPS) cells is expected to be a promising platform for elucidating the pathogenesis of the diseases and for drug discovery. (biomedcentral.com)
Investigating human immune-related diseases using induced pluripotent stem (iPS) cells presents a novel and promising field of study. (biomedcentral.com)
Antisera directed against histocompatibility-2 antigens of the mouse suppress the formation of spleen colonies by pluripotent hemopoietic stem cells. (tno.nl)

Though we haven't cured cancer, we've learned a lot about basic biology, cells, and genes. (pharmexec.com)
The DKFZ is a multidisciplinary center covering cancer biology from basic molecular and cell biology to clinical research and care. (mdc-berlin.de)
We have discovered novel insights into the biology of human T cell exhaustion and developed approaches to prevent and reverse this phenomenon. (stanford.edu)
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of The Digital Cell: Cell Biology as a Data Science, available on its website in hardcover format. (cshlpress.com)
The Emerging Cell Biology of Thyroid Stem Cells. (genescells.ru)

To address this knowledge gap, we developed an in vivo hematopoietic stem cell (HSC)-based large-scale CRISPR knockout screening platform to enable the genetic interrogation of hematopoiesis and broad aspects of immune cell function in vivo. (biorxiv.org)
Examples are CRISPR/Cas9-mediated mutagenesis, genetic mouse models, cell-fate-mapping, organoids and tumor explants, as well as GMP production of pharmaceuticals and many years of experience in conducting clinical studies. (mdc-berlin.de)
Our long-term objectives are to understand the genetic pathways that control human hematopoietic stem cells (HSCs) and to determine how changes in these programs lead to generating leukemic stem cells (LSC). (jdstemcellresearch.ca)
In addition to basic science investigations in such areas as tumor cell invasion, oncogenes, immunotherapy and genetic inheritance, our scientists and physicians participate in national cancer trials. (upstate.edu)
In particular, patient-derived iPS cells retain the same genetic background as these patients, allowing for recurrent analysis of genetic effects on cellular functions. (biomedcentral.com)
In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
Dr. Multani has extensive expertise in the field of Human and Mammalian Cytogenetics, and specializes in the evaluation of genetic instability and complex chromosomal rearrangements in cancer cells, telomere dysfunction, characterization of embryonic stem cells, and authentication of cell lines. (mdanderson.org)
Additional studies of apparently healthy adults characterizing longitudinal changes in clone size over time may reveal genetic and environmental factors promoting clonal stability versus progression and yield new insights into mechanisms underlying somatic mutagenesis and aging as well as resultant disease pathogenesis and disease prediction. (biomedcentral.com)
Rejuvenation therapies aim to reverse or repair age-related cellular changes such as molecular waste, calcification , tissue stiffening , loss of stem cell function , genetic alterations, and impaired energy production . (fightaging.org)

Unlike dysplastic clones in myelodysplastic syndrome (MDS), which harbour intrinsic defects that predispose the cells to clonal evolution, external factors, such as pressure exerted by the immune system, have for a long time been speculated to be the main causes for clonal escape in PNH [ 5 ]. (annexpublishers.co)

The Flt-3 ( fms- like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. (rndsystems.com)
Because Down syndrome leukemogenesis initiates during fetal development, we characterized the cellular and developmental context of preleukemic initiation and leukemic progression using gene editing in human disomic and trisomic fetal hematopoietic cells and xenotransplantation. (jdstemcellresearch.ca)

Recent clinical data indicate that patients with complex karyotypes, adverse genes, and high pre-infusion minimal residual disease (MRD) by flow cytometry in the bone marrow, were at high risk of relapse after CAR T-cell therapy. (biomedcentral.com)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

SAGA complex member expression is significantly reduced in aged HSCs and upregulated with heterochronic parabiosis, suggesting a novel mechanism of age-associated hematopoietic decline and rejuvenation. (biorxiv.org)
Bone marrow transplants are considered to be the first successful example of tissue engineering, a field within regenerative medicine that uses hematopoietic stem cells (HSCs) as a vehicle for treatment. (asu.edu)
Although inflammation dysregulates mature myeloid cells and influences stemness programs and lineage determination in HSCs by activating stress myelopoiesis, such roles in LSCs are poorly understood. (jdstemcellresearch.ca)
Lifelong blood production requires long-term hematopoietic stem cells (LT-HSCs), marked by stemness states involving quiescence and self-renewal, to transition into activated short-term HSCs (ST-HSCs) with reduced stemness. (jdstemcellresearch.ca)

We demonstrate that targeting the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein to CD40 (αCD40.RBD) induces significant levels of specific T and B cells, with long-term memory phenotypes, in a humanized mouse model. (nature.com)
Drugs that block the CXCR4 receptor appear to be capable of "mobilizing" hematopoietic stem cells into the bloodstream as peripheral blood stem cells . (wikidoc.org)
The entry of SARS-CoV-2 into host cells is facilitated by the binding of the spike protein on the virus surface to the cell surface receptor angiotensin-converting enzyme 2 (ACE2). (bvsalud.org)
Although anti-CD19 chimeric antigen receptor (CAR) T-cell therapy shows good efficacy in patients with relapsed/refractory B-cell acute lymphoblastic leukemia (r/r B-ALL), it fails to improve long-term leukemia-free survival (LFS). (biomedcentral.com)

Mesenchymal stem cells -Cells from the immature embryonic connective tissue. (cellmedicine.com)
A number of celltypes come from mesenchymal stem cells, includingchondrocytes, which produce cartilage. (cellmedicine.com)

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
CD31 is a 130 kDa integral membrane protein, a member of the immunoglobulin superfamily, that mediates cell-to-cell adhesion. (bdbiosciences.com)
They described two patients with PNH with an acquired rearrangement of chromosome 12 affecting the HMGA2 gene, which encodes a chromatin remodeling protein, in the PIGA mutant cells [ 12 ]. (annexpublishers.co)
[3] Ubiquitin is a small (76-amino acid) protein highly conserved among eukaryotic cells. (wikidoc.org)

Secondary myelofibrosis is due to implantation or invasion by malignant cancer cells that have metastasized because of implantation of blood-borne tumor cells from a distant cancer. (medscape.com)
Antisense oligonucleotide gapmers containing phosphoryl guanidine groups reverse MDR1-mediated multiple drug resistance of tumor cells. (yale.edu)
The tumor cells are clonal and characterized by an increased expression of several cellular oncogenes. (biomedcentral.com)
Typically, rapidly proliferating tumor cells have glycolytic rates up to 200 times higher than those of their normal tissue of origin, even in the presence of oxygen [ 3 ]. (hindawi.com)

Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
Specifically, methods are disclosed for mobilizing hematopoietic stem cells into a subject's peripheral blood. (justia.com)
In particular, embodiments of the method involve inhibition of both Rac1 and Rac2 GTPases to increase the numbers of hematopoietic stem cells into a subject's peripheral blood of a subject. (justia.com)
[ 2 ] to the presence of a few teardrop-shaped red blood cells and early myeloid precursor cells in the peripheral blood smear. (medscape.com)

Acute myeloid leukemia (AML) is a caricature of normal hematopoiesis driven from leukemia stem cells (LSC) that share some hematopoietic stem cell (HSC) programs including responsiveness to inflammatory signaling. (jdstemcellresearch.ca)
Clonal hematopoiesis of indeterminate potential (CHIP), the age-related expansion of mutant hematopoietic stem cells, confers risk for multiple diseases of aging including hematologic cancer and cardiovascular disease. (biomedcentral.com)

Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)
Bone marrow cells are completely replaced by large carcinoma cells with clear nucleoli. (medscape.com)
Leukemic cells, such as those occurring in chronic leukemias in which the expanding cells are mature and coexist peacefully with the normal bone marrow cells, show no evidence of myelophthisis, and marrow damage does not occur. (medscape.com)
Two-color flow cytometric analysis of CD31 expression on BALC/c mouse bone marrow cells. (bdbiosciences.com)
Bone marrow cells were stained with APC Rat Anti-Mouse CD45R/B220 antibody (Cat. (bdbiosciences.com)
Two-color flow cytometric dot plots show the correlated expression patterns of CD31 (or Ig Isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. (bdbiosciences.com)

The expanding number and volume of pathologic cells and the release of suppressive cytokines can eventually lead to bone marrow failure without the characteristic morphologic features of myelophthisis. (medscape.com)
A review is presented of the experiments that resulted in the identification of a specific morphologic entity representing the pluripotential hemopoietic stem cell (HSC) in mouse bone marrow. (tno.nl)

The Goodell Lab focuses on the basic mechanisms that regulate hematopoietic stem (HSC) cells, and how their mis-regulation leads to leukemia development. (chicagobiomedicalconsortium.org)
On the subject of acute myeloid leukemia (AML), there is little consensus about the target cell within the hematopoietic stem cell hierarchy that is susceptible to leukemic transformation, or about the mechanism that underlies the phenotypic, genotypic and clinical heterogeneity. (scienceopen.com)
Here we demonstrate that the cell capable of initiating human AML in non-obese diabetic mice with severe combined immunodeficiency disease (NOD/SCID mice) - termed the SCID leukemia-initiating cell, or SL-IC - possesses the differentiative and proliferative capacities and the potential for self-renewal expected of a leukemic stem cell. (scienceopen.com)
CC-90009, a novel cereblon E3 ligase modulator, targets acute myeloid leukemia blasts and leukemia stem cells. (jdstemcellresearch.ca)
Depletion of GSPT1 by CC-90009 rapidly induces acute myeloid leukemia (AML) apoptosis, reducing leukemia engraftment and leukemia stem cells (LSCs) in large-scale primary patient xenografting of 35 independent AML samples, including those with adverse risk features. (jdstemcellresearch.ca)
Here we describe the phenotypic and molecular characteristics of the B cell leukemia/lymphoma in the Tg mice. (biomedcentral.com)
Flow cytometry was used to measure the uptake of adriamycin (AM) and daunomycin (DM) by various cell types of the haemopoietic organs and by acute myeloid leukemia (AML) cells. (tno.nl)
Early loss or exhaustion of CAR T cells, selection of CD19-negative clones, downregulation of CD19 expression, lineage switch of leukemia, and tumor microenvironment are important factors contributing to relapse after CAR T-cell therapy [ 9 , 10 ]. (biomedcentral.com)
The combination of CAR T-cell therapy and allo-HSCT has been suggested to reduce the relapse rate of leukemia. (biomedcentral.com)

Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
Expression of B cell-stimulatory cytokines IL-1β, IL-6, IL-10, IL-12 p40 , IL-13 and TNFα and HIV proteins p17, gp120 and nef were elevated in the Tg mice with lymphoma. (biomedcentral.com)
We explored the steady-state stromal structure of lymph nodes isolated from mice and human beings and discovered that marginal reticular cells and lymphatic endothelial cells needed lymphocytes for his or her regular maturation in mice. (immune-source.com)
Enzymatic digestive function of lymph nodes from specific mice For movement cytometric evaluation or cell tradition lymph nodes from specific mice had been dissected pierced once with good forceps and put into 5?ml of RPMI-1640 on snow. (immune-source.com)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)

We show that germline SBDS deficiency establishes a fitness constraint that drives selection of somatic clones via two distinct mechanisms with different clinical consequences. (nature.com)
Recently developed technologies produce clinical quality cells in as few as12 days. (pharmexec.com)
Stem cell-based therapeutics that enter clinical trials within the next five years will likely enter clinical practice in the next 10 years. (pharmexec.com)
In a small human clinical trial to evaluate the safety and efficacy of fucoidan ingestion (brown seaweed extract), 3g daily of 75% w/w oral fucoidan for 12 days increased the proportion of CD34+CXCR4+ from 45 to 90% and the serum SDF-1 levels, which could be useful in CD34+ cells homing/mobilization via SDF-1/CXCR4 axis. (wikidoc.org)
Current Research and Scholarly Interests Recent clinical studies, by us and others, have demonstrated that genetically engineered T cells can eradicate cancers resistant to all other therapies. (stanford.edu)
Stem cell senescence in the in vitro expansion stage is an essential concern in clinical procedures of cell therapy. (magiran.com)
Allo-HSCT provides optimal clinical benefit in patients with MRD-negative complete remission, typically within three months after CAR T-cell therapy. (biomedcentral.com)
Herein, we summarize the clinical data on consolidative allo-HSCT after anti-CD19 CAR T-cell therapy, as well as the potential factors associated with allo-HSCT benefit. (biomedcentral.com)
This review describes current considerations in the use of hydroxyurea for the management of sickle cell disease in the context of clinical severity. (cdc.gov)

Since its discovery in 2000, initial in vitro studies have found that the engagement of Siglec-8 with a monoclonal antibody or with selective polyvalent sialoglycan ligands induced the cell death of eosinophils and inhibited mast cell degranulation. (mdpi.com)
Cell culture -Growth of cells in vitro on an artificial medium for experimental research. (cellmedicine.com)
In vitro assays that permit cloning of tumour cells in soft agar have been improved during the last 5 years. (tno.nl)
Adherent cells from mouse bone marrow have been shown to promote the in vitro growth of the AVRij-1 tumour cell line. (tno.nl)

Molecular cloning of a novel human gene on chromosome 4p11 by immunoscreening of an ovarian carcinoma cDNA library. (nih.gov)
Human KRML (MAFB): cDNA cloning, genomic structure, and evaluation as a candidate tumor suppressor gene in myeloid leukemias. (atlasgeneticsoncology.org)

T lymphocytes for adoptive cell therapy against hematological cancer. (upstate.edu)
Increasing natural killer (NK) cell effector functions for the treatment of pediatric hematological cancer. (biocrucesbizkaia.org)

Long-term self-renewal -The ability of stem cells to renew themselves by dividing into the same non-specialized cell type over long periods (many months to years) depending on the specific type of stem cell. (cellmedicine.com)
The memory B cell-enriched transcripts were then compared with memory T cell-enriched and hematopoietic stem cell-enriched transcripts in order to generate a transcriptional profile of self-renewal within the hematopoietic system. (omicsdi.org)

As of 2009, West advocated using human somatic cell nuclear transfer techniques to derive human embryonic stem cells for therapeutic practice. (asu.edu)

Overview of all the structural information available in the PDB for UniProt: O43324 (Human Eukaryotic translation elongation factor 1 epsilon-1 (EEF1E1)) at the PDBe-KB. (wikipedia.org)
Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells. (nih.gov)
Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. (rndsystems.com)
Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
New research published in Nature Medicine on January 23 highlights yet another challenge: The cell lines currently approved for study under federal funding contain a non-human molecule, the sialic acid Neu5Gc, which elicits an immune response in humans. (pharmexec.com)
The HeLa cell line was the first immortal human cell line that George Otto Gey, Margaret Gey, and Mary Kucibek first isolated from Henrietta Lacks and developed at The Johns Hopkins Hospital in Baltimore, Maryland, in 1951. (asu.edu)
An immortal human cell line is a cluster of cells that continuously multiply on their own outside of the human from which they originated. (asu.edu)
Scientists use immortal human cell lines in their research to investigate how cells function in humans. (asu.edu)
Yamanaka induced somatic cells to act like human embryonic stem cells (hESCs), allowing researchers to experiment with non-embryonic stem cells with a similar capacity as hESCs. (asu.edu)
His focus has ranged from the development of telomerase-based therapeutics to the application of human embryonic stem cells in regenerative medicine. (asu.edu)
James Alexander Thomson, affectionately known as Jamie Thomson, is an American developmental biologist whose pioneering work in isolating and culturing non-human primate and human embryonic stem cells has made him one of the most prominent scientists in stem cell research. (asu.edu)
It is a very efficient inducer of hematopoietic stem cell mobilization in animal and human studies. (wikidoc.org)
A latent subset of human hematopoietic stem cells resists regenerative stress to preserve stemness. (jdstemcellresearch.ca)
The Transition from Quiescent to Activated States in Human Hematopoietic Stem Cells Is Governed by Dynamic 3D Genome Reorganization. (jdstemcellresearch.ca)
Human Immunodeficiency Virus Type I (HIV-1) infection is associated with a high incidence of B-cell lymphomas. (biomedcentral.com)
Human Immunodeficiency Virus Type I (HIV-1) infection is associated with an elevated incidence of B-cell non-Hodgkin's lymphoma (NHL) and in recent years also with Hodgkin's lymphoma [ 1 ]. (biomedcentral.com)
Murine models for human AIDS-related B cell lymphomas have been lacking. (biomedcentral.com)
The potential use of embryonic stem cells (ESCs) for cell replacement therapies is limited by ethical concerns and the technical hurdles associated with their isolation from human embryos. (biomedcentral.com)
For staining human being cells antibodies utilized had been: anti-CD45 (clone HI30 Biolegend) anti-CD31 (clone WM59 BD Biosciences) and anti-podoplanin (clone NZ-1 AngioBio Co) recognized with extremely cross-adsorbed anti-rat IgG (H? (immune-source.com)
We suggest an advantage of utilizing iPS cells for studying human immune-related diseases. (biomedcentral.com)
With iPS cell technology, researchers can readily acquire human cells and investigate these diseases using human-derived samples. (biomedcentral.com)
In this way, investigating human immune-related diseases using iPS cells offers distinct advantages over conventional research methods. (biomedcentral.com)
In this review, we discuss the application of iPS cells in the study of human immune-related diseases, with an emphasis on autoimmune diseases (Fig. 1 ). (biomedcentral.com)
Cell population kinetics in dog and human adult thyroid. (genescells.ru)
Ma R., Latif R., Davies T.F. Human embryonic stem cells form functional thyroid follicles. (genescells.ru)
Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)

These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
It's important to remember that in addition to getting past the political stigma, developing stem cell-based therapies is really no different than any other biotech endeavor. (pharmexec.com)
Beck predicts that stem cell-based therapies will also come to market in a systematic fashion. (pharmexec.com)
We are identifying new targets for these therapeutics, exploring pathways of resistance to current cell therapies and creating next generation platforms to overcome therapeutic resistance. (stanford.edu)

The MEC13.3 antibody specifically recognizes CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1). (bdbiosciences.com)

In addition to the aging phenomenon, stem cell senescence has been investigated in various concepts such as cancer, adverse drug effects, and as a limiting factor in cell therapy. (magiran.com)
A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. (hindawi.com)
High pre-lymphodepletion lactate dehydrogenase, low pre-lymphodepletion platelet count, absence of fludarabine in lymphodepletion, persistent leukemic sequence by high throughput sequencing in bone marrow after CAR T-cell infusion, and early loss of CAR T cells have also been linked to relapse after CAR T-cell therapy. (biomedcentral.com)
In patients having these risk factors, consolidative allo-HSCT after CAR T-cell therapy may prolong LFS. (biomedcentral.com)
Finally, and most importantly, we provide recommendations for the assessment and management of r/r B-ALL patients undergoing anti-CD19 CAR T-cell therapy. (biomedcentral.com)

How is the tissue specificity of immune cells generated and how is it affected by environmental influences, plasticity and the tissue microenvironment? (mdc-berlin.de)
Adult stem cell -An undifferentiated cell found in a differentiated tissue that can renew itself and (with certain limitations) differentiate to yield all the specialized cell types of the tissue from which it originated. (cellmedicine.com)
The review presents literature data on embryogenesis, physiological and reparative thyroid gland (TG) regeneration in the postnatal period in terms of substantiating the presence of stem/progenitor cells in its tissue. (genescells.ru)
Unresolved problems and perspectives of the use of thyroid stem/progenitor cells in tissue engineering are discussed. (genescells.ru)

The SL-ICs from all subtypes of AML analyzed, regardless of the heterogeneity in maturation characteristics of the leukemic blasts, were exclusively CD34++ CD38-, similar to the cell-surface phenotype of normal SCID-repopulating cells, suggesting that normal primitive cells, rather than committed progenitor cells, are the target for leukemic transformation. (scienceopen.com)
The SL-ICs were able to differentiate in vivo into leukemic blasts, indicating that the leukemic clone is organized as a hierarchy. (scienceopen.com)

In this paper, we describe the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, underlying the connection between these two processes. (hindawi.com)

Lipids, amino acids, and nucleotides necessary for the biosynthesis of the daughter cells are mostly provided by intermediate metabolites of these pathways. (hindawi.com)

They can also be used for increasing white blood cell survival and for chemotherapy. (justia.com)
[12] [13] However, in breast cancer where SDF1/CXCL12 is also expressed by the cancer cells themselves along with CXCR4, CXCL12 expression is positively correlated with disease free (metastasis free) survival. (wikidoc.org)
TRM relates to patient characteristics and general health while drug resistance or overall survival is characteristic of the AML clone. (ghrnet.org)

Siglecs (sialic acid-binding immunoglobulin-like lectins) are single-pass cell surface receptors that have inhibitory activities on immune cells. (mdpi.com)
PNH clones are present in a majority of patients with aplastic anemia, which is consistent with the strong immune component in the pathogenesis of this disease [ 7-9 ]. (annexpublishers.co)
One of the research areas at the MDC is immunology and inflammation, which covers tumor immunology, DNA repair in B lymphocytes, the role of tumor stroma-immune cell interaction in cancers, the role of innate immune cells in gliomas and neurodegenerative diseases, and the development of immunotherapy strategies. (mdc-berlin.de)
Continuous supply of immune cells throughout life relies on the delicate balance in the hematopoietic stem cell (HSC) pool between long-term maintenance and meeting the demands of both normal blood production and unexpected stress conditions. (jdstemcellresearch.ca)
In summary, patient-derived iPS cells can provide a promising platform for pathological research and new drug discovery in the field of immune-related diseases. (biomedcentral.com)
A schematic summary of the investigation of immune-related diseases using patient-derived iPS cells. (biomedcentral.com)
During the process of airway inflammation, complex interactions of innate and adaptive immune cells as well as structural cells and their cytokines have many important roles. (cdc.gov)

In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)

This blood film at 1000X magnification demonstrates a leukoerythroblastic blood picture with the presence of precursor cells of the myeloid and erythroid lineage. (medscape.com)
Agnogenic myeloid metaplasia is a stem cell abnormality associated with myeloproliferative diseases. (medscape.com)

The transformed B cell population consists of CD19 + pre-BCR + CD127 + CD43 + CD93 + precursor B cells. (biomedcentral.com)

Biochemical, structural, and molecular characterization demonstrates that CC-90009 coopts the CRL4CRBN to selectively target GSPT1 for ubiquitination and proteasomal degradation. (jdstemcellresearch.ca)

Another reason is that protocols for differentiating some lymphocytes associated with autoimmunity, such as CD4 + T cells or B cells, from iPS cells have not been well established. (biomedcentral.com)

Infiltrating lesions caused by nonhematopoietic cells invading bone marrow can result in varying degrees of cytopenia, including anemia , thrombocytopenia, neutropenia, and pancytopenia. (medscape.com)

Immunoreceptor signaling during development, homeostasis, and effector function of T cells and NK cells. (upstate.edu)

Aberrant expression of a third marker such as Thy-1/CD90 negativity, expression of CLL-1 and IL-3R (CD123), intermediate levels of aldehyde dehydrogenase and co-expression of common chromosomal translocation may be used to distinguish from normal hematopoietic stem cells. (ghrnet.org)

Anatomy45

Organisms14

Diseases27

Chemicals and Drugs59

Analytical, Diagnostic and Therapeutic Techniques and Equipment56

Psychiatry and Psychology1

Phenomena and Processes51

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Named Groups3

Health Care6

Differentiation22

Transplantation9

Mutations13

Normal hematopoietic cells2

Progenitor cell4

Stromal cells1

Tissues9

Fibroblasts4

Clonal expansion2

Proliferation10

Tumors4

Embryonic stem6

Reconstitution2

Pluripotent11

Biology5

Genetic9

Myelodysplastic syndrome1

Fetal2

Genes2

HSCs4

Receptor4

Mesenchymal2

Protein4

Tumor cells4

Peripheral blood4

Hematopoiesis2

Bone marro6

Morphologic2

Leukemia9

Mice5

Clinical9

Vitro4

CDNA2

Hematological2

Self-renewal2

Somatic cell nuc1

Human26

Therapies4

Endothelial1

Therapy5

Tissue4

Blasts2

Apoptosis1

Pathways1

Survival3

Immune7

Undergo1

Myeloid2

CD191

Characterization1

Lymphocytes1

Cytopenia1

Homeostasis1

Chromosomal1