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  • severe
  • [ 2 , 8 ] Survivors of bone marrow transplantation were more likely to have a severe or life threatening condition (relative risk [RR] = 3.9), more than one chronic condition (RR = 2.6), functional impairment (RR=3.5), and activity limitations (RR = 5.8) than conventionally treated patients. (medscape.com)
  • Severe sickle cell crises, in some cases resulting in death, have been associated with the use of filgrastim in patients with sickle cell disorders. (wikipedia.org)
  • This condition can rapidly lead to life-threatening infection, as the body cannot produce leukocytes in response to invading bacteria and viruses, as well as leading to anaemia due to a lack of red blood cells and spontaneous severe bleeding due to deficiency of platelets. (wikipedia.org)
  • Parvovirus B19 inhibits erythropoiesis by lytically infecting RBC precursors in the bone marrow and is associated with a number of different diseases ranging from benign to severe. (wikipedia.org)
  • There he contributed to the isolation of the blood stimulating growth factor G-CSF, which leads to a faster recovery of white blood cells after chemotherapy and radiation therapy protecting patients better against severe infections. (wikipedia.org)
  • acute
  • V-25 Leukemic cells in acute lymphoblastic leukemia characterized by round or convoluted nuclei, high nuclear/cytoplasmic ratio and absence of cytoplasmic graulnes. (powershow.com)
  • By using all techniques available at the time for phenotypic characterization of leukemia cells, he was successful in discovering and describing novel subentities of acute leukemias. (wikipedia.org)
  • precursors
  • Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. (wikipedia.org)
  • eosinophils
  • Clonal hypereosinophilia, also termed Primary hypereosinophelia or clonal eosinophilia, is a grouping of hematological disorder characterized by the development and growth of a pre-malignant or malignant population of eosinophils, a type of white blood cell, in the bone marrow, blood, and/or other tissues. (wikipedia.org)
  • This population consists of a clone of eosinophils, i.e. a group of genetically identical eosinophils derived from a sufficiently mutated ancestor cell. (wikipedia.org)
  • The clone of eosinophils bear a mutation in any one of several genes that code for proteins that regulate cell growth. (wikipedia.org)
  • The expanding population of eosinophils, initially formed in the bone marrow may spread to the blood and then enter into and injure various tissues and organs. (wikipedia.org)
  • Some hematological malignancies exhibit increased numbers of circulating blood eosinophils, increased numbers of bone marrow eosinophils, and/or eosinophil infiltrations into otherwise normal tissues. (wikipedia.org)
  • 2016
  • In 2006 Eaves retired as required by provincial law at that time, becoming Professor Emeritus of Hematology and spending more time on several companies he founded to further the fields of cellular therapy and regenerative medicine - STEMCELL Technologies, STEMSOFT Software Inc. and Malachite Management Inc. These companies continue to grow under his leadership and by 2016, STEMCELL had over 900 employees making Canada's largest biotechnology company. (wikipedia.org)
  • In 2016, STEMCELL was awarded overall Exporter of the Year, as well as won in the Advancing Technology & Innovation category, while Dr. Eaves won in the Leadership category at the BC Export Awards. (wikipedia.org)
  • Clinical
  • This is a study to investigate the potential clinical benefit of trilaciclib (G1T28) in preserving the bone marrow and the immune system, and enhancing chemotherapy antitumor efficacy when administered prior to carboplatin and gemcitabine (GC therapy) for patients with metastatic triple negative breast cancer. (moffitt.org)
  • In-vitro colony forming cell (CFC) assays using normal human bone marrow grown in appropriate semi-solid media such as ColonyGEL have been shown to be useful in predicting the level of clinical myelotoxicity a certain compound might cause if administered to humans. (wikipedia.org)
  • The safety and efficacy of Caphosol has been demonstrated in a double-blind prospective, randomized clinical trial in patients undergoing bone marrow transplantation. (wikipedia.org)
  • Hematology
  • Allen C. Eaves (born 1941) was the founding Director of the Terry Fox Laboratory for Hematology/Oncology Research, which over a 25 year period (1981-2006) he grew into an internationally recognized[citation needed] centre for the study of leukemia and stem cell research. (wikipedia.org)
  • Mertelsmann is known for his scientific works in the fields of hematology, oncology, gene therapy and stem cell transplantation. (wikipedia.org)
  • At Freiburg University Medical Center he became the director of the Department for Medicine I, Oncology, Hematology and Stem Cell Transplantation. (wikipedia.org)
  • chronic
  • miR-15 and miR-16 genes are often deleted or expressed at reduced levels in B cell chronic lymphocytic leukemias (CLL), suggesting that these miRNAs might be tumor-suppressor genes ( 25 - 27 ). (pnas.org)
  • Eaves' personal research has focused on leukemia where he pioneered "culture purging" as a novel approach to doing autologous bone marrow transplantation for chronic myelogenous leukemia. (wikipedia.org)
  • In immunocompromised patients, B19 infection may persist for months, leading to chronic anemia with B19 viremia due to chronic marrow suppression. (wikipedia.org)
  • Absent these causes, patients were diagnosed in the World Health Organization's classification as having either 1) Chronic eosinophilic leukemia, not otherwise specified, (CEL-NOS) if blood or bone marrow blast cells exceeded 2% or 5% of total nucleated cells, respectively, and other criteria were met or 2) idiopathic hypereosinophilic syndrome (HES) if there was evidence of eosinophil-induced tissue damage but no criteria indicating chronic eosinophilic leukemia. (wikipedia.org)
  • Blood
  • Origin electricity faults 1, 2, 3, 4 The annual activity survey of the European Society of Blood and Marrow Transplantation (EBMT), describing the status of HSCT in Europe and affiliated countries, has become an instrument used to observe trends and to monitor changes in technology use. (gaselectricity.in)
  • Recipients will provide an initial donation of white blood cells to be used for research purposes only. (clinicaltrials.gov)
  • After the initial chemotherapy, participants will receive the donated white blood cells and stem cells as a single infusion. (clinicaltrials.gov)
  • is a disease in which too many underdeveloped infection-fighting white blood cells, called lymphocytes, are found in a child's blood and bone marrow. (powershow.com)
  • These abnormal cells reproduce very quickly and do not function as healthy white blood cells to help fight infection. (powershow.com)
  • Peripheral blood anemia,thrombocytopenia, variable white cell count with or without blasts. (powershow.com)
  • blood tests to count the number of each of the different kinds of blood cells. (powershow.com)
  • We are using genetic and reprogramming approaches to instruct iPSCs to create these clinically valuable blood cells. (washington.edu)
  • In recognition of his research accomplishments and leadership in moving basic science discoveries in stem cell biology into the clinic, he was elected President of the International Society of Cellular Therapy (1995-1997), Treasurer of the Foundation for the Accreditation of Cellular Therapy (1995-2002) and President of the American Society of Blood and Marrow Transplantation (1999-2000). (wikipedia.org)
  • It may also be used to increase white blood cells for gathering during leukapheresis. (wikipedia.org)
  • Filgrastim is also used to increase the number of hematopoietic stem cells in the blood before collection by leukapheresis for use in hematopoietic stem cell transplantation. (wikipedia.org)
  • Bone marrow suppression also known as myelotoxicity or myelosuppression, is the decrease in production of cells responsible for providing immunity (leukocytes), carrying oxygen (erythrocytes), and/or those responsible for normal blood clotting (thrombocytes). (wikipedia.org)
  • The decrease in blood cell counts does not occur right at the start of chemotherapy because the drugs do not destroy the cells already in the bloodstream (these are not dividing rapidly). (wikipedia.org)
  • Instead, the drugs affect new blood cells that are being made by the bone marrow. (wikipedia.org)
  • Because the bone marrow is the manufacturing center of blood cells, the suppression of bone marrow activity causes a deficiency of blood cells. (wikipedia.org)
  • These predictive in-vitro assays reveal effects the administered compounds have on the bone marrow progenitor cells that produce the various mature cells in the blood and can be used to test the effects of single drugs or the effects of drugs administered in combination with others. (wikipedia.org)
  • CD34+ cells may be isolated from blood samples using immunomagnetic or immunofluorescent methods. (wikipedia.org)
  • G-CSF stimulates the production of granulocytes, a type of white blood cell. (wikipedia.org)
  • Hematopoietic stem cells (HSCs) are the stem cells that give rise to blood cells through the process of haematopoiesis. (wikipedia.org)
  • It must balance enormous production needs (more than 500 billion blood cells are produced every day) with the need to precisely regulate the number of each blood cell type in the circulation. (wikipedia.org)
  • Dose
  • Early research in bone marrow transplantation by E. Donnall Thomas and colleagues demonstrated that this process of splitting TBI into multiple smaller doses resulted in lower toxicity and better outcomes than delivering a single, large dose. (wikipedia.org)
  • bloodstream
  • Granulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. (wikipedia.org)
  • radiation
  • Radiation therapy (also called radiotherapy, X-ray therapy, or irradiation) is the use of ionizing radiation to kill cancer cells and shrink tumors. (wikipedia.org)
  • Radiation therapy injures or destroys cells in the area being treated (the "target tissue") by damaging their genetic material, making it impossible for these cells to continue to grow and divide. (wikipedia.org)
  • Although radiation damages both cancer cells and normal cells, most normal cells can recover from the effects of radiation and function properly. (wikipedia.org)
  • lymphocytes
  • Most of the identifiable cells are lymphocytes or plasma cells. (medscape.com)
  • B cells are derived from a common lymphoid progenitor (CLP) cell (lin − c- kit low Sca-1 low IL-7R + ) ( 1 ), which is limited in its ability to develop into T lymphocytes but is fully capable of developing into B cells. (pnas.org)
  • or 2) lymphoid precursor cells which differentiate into T lymphocytes, B lymphocytes, or natural killer cells. (wikipedia.org)
  • infection
  • Bone marrow suppression due to anti-cancer chemotherapy is much harder to treat and often involves hospital admission, strict infection control, and aggressive use of intravenous antibiotics at the first sign of infection. (wikipedia.org)
  • tissue
  • The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue. (wikipedia.org)
  • For this reason, the pathologist will examine the surgical specimen to determine if a margin of healthy tissue is present, thus decreasing the chance that microscopic cancer cells are left in the patient. (wikipedia.org)
  • dendritic
  • We have provided direct and novel evidence for the migration of bone marrow and HSC cells into the sclera differentiating into macrophages and dendritic cells. (pubmedcentralcanada.ca)
  • Recently, the presence of APCs such as macrophages and dendritic cells was reported in the cornea. (pubmedcentralcanada.ca)
  • 15 , 16 The CD45+/CD11b+/CD11c+ dendritic cells distributed in corneal epithelium 8 and corneal stroma, 9 , 17 and CD11b+/CD11c‐ macrophages in the corneal stroma. (pubmedcentralcanada.ca)
  • Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. (wikipedia.org)
  • fetal
  • In mammalian embryology, the first definitive HSCs are detected in the AGM (aorta-gonad-mesonephros), and then massively expanded in the fetal liver prior to colonising the bone marrow before birth. (wikipedia.org)
  • lymphomas
  • However, miR-17-92 and miR-155 are highly expressed in B cell lymphomas ( 28 , 29 ), and miR-17-92 can function as an oncogene when ectopically expressed with c-Myc ( 28 ). (pnas.org)
  • stimulates
  • Instead, it is a highly conserved process that promotes the context dependent elimination of less fit cells and stimulates growth of more fit cells during growth and homeostasis. (keystonesymposia.org)
  • murine
  • Publications] Ogawa M: 'Expression and function of c-kit in fetal hemopoietic progenitor cells:transtion from the early c-kit-independent to the late c-kit:dependent wave of hemopoiesis in the murine embryo. (nii.ac.jp)
  • Trib2 had no effect on the growth and survival of murine T-ALL cell lines in vitro when expression was blocked by shRNAs. (hematopoiesisnews.com)
  • malignant
  • Hematopoietic stem cell transplantation for children with bone marrow failure syndromes, immune deficiencies, malignant and inherited disorders. (fnkc.ru)
  • Malignant infantile osteopetrosis, also known as infantile autosomal recessive osteopetrosis or simply infantile osteopetrosis is a rare osteosclerosing type of skeletal dysplasia that typically presents in infancy and is characterized by a unique radiographic appearance of generalized hyperostosis - excessive growth of bone. (wikipedia.org)
  • Failure of remodeling of the distal femoral and proximal humeral metaphyses giving the affected bones a funnel shaped appearance known as (Erlenmeyer flask deformity) Alternating radiolucent femoral metaphyseal bands Pathologic fractures The differential diagnosis of malignant infantile osteopetrosis includes other genetic skeletal dysplasias that cause osteosclerosis. (wikipedia.org)
  • Neuropathic infantile osteopetrosis Infantile osteopetrosis with renal tubular acidosis Infantile osteopetrosis with immunodeficiency IO with leukocyte adhesion deficiency syndrome (LAD-III) Intermediate osteopetrosis Autosomal dominant osteopetrosis (Albers-Schonberg) Pyknodysostosis (osteopetrosis acro-osteolytica) Osteopoikilosis (Buschke-Ollendorff syndrome) Osteopathia striata with cranial sclerosis Mixed sclerosing bone dysplasia Progressive diaphyseal dysplasia (Camurati-Engelmann disease) SOST-related sclerosing bone dysplasias The only effective line of treatment for malignant infantile osteopetrosis is hematopoietic stem cell transplantation. (wikipedia.org)
  • citation needed] Radiographs of at least one case with malignant infantile osteopetrosis have demonstrated bone remodeling and recanalization of medullar canals following hematopoietic stem cell transplantation. (wikipedia.org)
  • citation needed] A major focus of McCulloch's more recent research has been on cellular and molecular mechanisms affecting the growth of malignant blast stem cells obtained from the blood of patients with Acute Myeloblastic Leukemia. (wikipedia.org)
  • malignancies
  • The non-dilutive funding will support Gamida Cell's ongoing research and development efforts including its Phase III registration study of NiCord ® for hematological malignancies (blood cancers like leukemia and lymphoma), and its clinical trials of CordIn ® for sickle cell disease and thalassemia and NK cells as a potential immune therapy for cancer. (hematopoiesisnews.com)
  • Tissue
  • As expected, some transgenic mice bearing 2kb or 6kb constructs showed tissue specific expression of LacZ gene in hair follicles, gut pacemaker cells and neurons where KL is expressed. (nii.ac.jp)
  • We expect that other mAb will be also useful to dessect the tissue structure of hematopoietic organs. (nii.ac.jp)
  • The methodology to create induced pluripotent stem cells (iPSCs) affords the opportunity to generate cells specific to the individual providing the host tissue. (jove.com)
  • Once iPSC colonies exhibit typical human embryonic stem cell (hESC) morphology, they are gently transferred to individual iMEF-coated tissue culture plates for continued growth and expansion. (jove.com)
  • HSCs constitute 1:10.000 of cells in myeloid tissue. (wikipedia.org)
  • clinical
  • BPDCN are characterized by co-expression of CD4 and CD56 antigens in the absence of any specific myeloid or lymphoid lineage or markers, primary skin infiltrations followed by bone marrow involvement, and aggressive clinical course. (czytelniamedyczna.pl)
  • However, in the past a number of different terms were used for BPDCN due to their uncommon immunophenotypic, histopathological and clinical features, including blastic NK leukemia/lymphoma, histiocytic lymphoma or histiocytic associated hematologic malignancy, cutaneous a granular CD4+ CD56+ hematodermic neoplasm and NK-cell lymphoma or myelo-monocytic precursor - related lymphoma (5-12). (czytelniamedyczna.pl)
  • Minimal morphological criteria for defining bone marrow dysplasia: a basis for clinical implementation of WHO classification of myelodysplastic syndromes. (hunimed.eu)
  • On the other hand, GM-CSF deficiency leads to various immune dysfunctions and the current utilization of GM-CSF as haematopoietic factor might be an accurate but very incomplete indication for a cytokine with vast clinical potential. (springer.com)
  • Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. (wikipedia.org)
  • induces
  • Background- Previous studies have shown that adrenomedullin (AM) inhibits vascular endothelial cell apoptosis and induces angiogenesis. (ahajournals.org)
  • patients
  • however, some patients fail to respond to this cell therapy. (ahajournals.org)
  • Annually, over 2,000 new patients are admitted, and more than 200 hematopoietic stem cell transplantations and 2,000 surgeries are performed in our Center. (fnkc.ru)
  • Further characterization revealed that these patients were neutropenic and NK cell deficient. (jci.org)
  • Interim results also showed that all but one patient with a non-β0/β0 genotype (12 of 13 patients) stopped receiving regular red blood cell (RBC) transfusions, with a median time since last transfusion of 27 months. (healthcanal.com)
  • These important results show that gene therapy is a promising new option for reducing or eliminating blood transfusions and limiting long-term complications of transfusions in patients with this disease," says Dr. Mark C. Walters, Director of the Blood & Marrow Transplantation (BMT) program at UCSF Benioff Children's Hospital Oakland and lead investigator of the Northstar study. (healthcanal.com)
  • The authors performed a prospective, nonrandomized Phase II study including 34 patients with multiple myeloma (MM) to evaluate bendamustine's efficacy and safety as a stem cell mobilizing agent. (hematopoiesisnews.com)
  • Serious side effects included severe myelosuppression (suppressed activity of bone marrow, which is involved in formation of various blood cells [found in 98% of patients]), disorder of the respiratory system, tumor lysis syndrome, Type III hypersensitivity, venous occlusion, and death. (wikipedia.org)
  • Later the onset of VOD was shown to occur at increased frequency in Gemtuzumab patients even following bone marrow transplantation. (wikipedia.org)
  • Patients with RD have a genetic defect that affects the T cells and at least one other type of immune cell. (wikipedia.org)
  • Patients who suffer from RD will now have more stem cells that can differentiate into immune cells. (wikipedia.org)
  • This cure is commonly used in patients who are awaiting bone marrow transplantation. (wikipedia.org)
  • This usually involves inserting a needle into the back of the patients hip bone and removing the sample from there. (wikipedia.org)
  • dendritic cells
  • Conti L, Gessani S. GM-CSF in the generation of dendritic cells from human blood monocyte precursors: recent advances. (springer.com)
  • While cartilage, ligaments, and fat present low antigenicity, bone, muscles, nerves, and vessels present moderate antigenicity, and skin is the component that develops the most severe rejection because of the abundance of dendritic cells within the epidermis and dermis. (springer.com)
  • therapy
  • When these findings are taken together, combination therapy with MNC transplantation and AM infusion may have additional or synergetic effects on therapeutic angiogenesis for the treatment of severe peripheral vascular disease. (ahajournals.org)
  • A novel approach to characterize the microstructural response of the myocardium to ischemia and cell therapy, using serial diffusion tensor MRI tractography of the heart in vivo , is presented. (celltherapynews.com)
  • McCulloch's work revolutionised cell biology and cancer therapy with the discovery of stem cells in the hematopoietic system. (wikipedia.org)
  • protein
  • Suppression of STAT3 NH 2 -terminal domain chemosensitizes medulloblastoma cells by activation of protein inhibitor of activated STAT3 via de-repression by microRNA-21. (nih.gov)
  • Cotransfection of an episome that expresses enhanced green fluorescent protein (eGFP) allows for easy identification of transfected cells. (jove.com)
  • Canine periodontal ligament stem cells were transduced with adenoviral vectors containing bone morphogenetic protein 2 (BMP2/PDLSCs). (celltherapynews.com)
  • The two cell types were transfected with green fluorescent protein to evaluate their fate after implantation in an intraarticular environment of the knee joint. (celltherapynews.com)
  • AK2 protein allows hematopoietic stem cells to differentiate and proliferate. (wikipedia.org)
  • If there is a mutation in the protein, that means that the mitochondria metabolism most likely will be altered and will not be able to provide enough energy to the hematopoietic stem cells. (wikipedia.org)
  • epigenetic
  • In addition, post-meiotic germ cells undergo a dramatic morphological reshaping process as well as a global epigenetic reconfiguration of the germ line chromatin-the histone-to-protamine switch. (jove.com)
  • These rearrangements result in increased expression of blood cell development genes by promoting gene transcription and through epigenetic changes. (wikipedia.org)
  • spleen
  • Till and McCulloch called the nodules 'spleen colonies', and speculated that each nodule arose from a single marrow cell: perhaps a stem cell. (wikipedia.org)
  • Link to article] Becker, A.J., McCulloch, E.A., Till, J.E. (1963) Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells. (wikipedia.org)
  • gene
  • We found that 35bp upstream of TATA-box is sufficient for assigning CAT-gene expression in this cell line. (nii.ac.jp)
  • These phenotypes were unexpectedly linked to mutations in the gene encoding a subunit of the Go-Ichi-Ni-San (GINS) complex, which is essential for DNA replication prior to cell division. (jci.org)
  • Resulting iPSCs were further characterized and deemed free of transfected DNA, integrated transgene DNA, and lack detectable gene rearrangements such as those within the immunoglobulin heavy chain and T cell receptor loci of more differentiated cell types. (jove.com)
  • LentiGlobin BB305, produced by BlueBird Bio, uses a non-communicable virus to deliver a fully functioning HBB gene to a patient's own blood-producing stem cells. (healthcanal.com)
  • This Phase I/II study has examined if using LentiGlobin BB305 to introduce a fully functioning hemoglobin gene into a patient's stem cells was a safe and effective treatment. (healthcanal.com)
  • Then in laboratory conditions the stem cells are manipulated and corrected with gene technology. (wikipedia.org)
  • The cortex is the location of the earliest events in thymocyte development, where T-cell receptor gene rearrangement and positive selection takes place. (wikipedia.org)
  • Thymocytes that reach the medulla have already successfully undergone T-cell receptor gene rearrangement and positive selection, and have been exposed to a limited degree of negative selection. (wikipedia.org)
  • endothelial cells
  • In vivo, AM administration not only enhanced the differentiation of MNC into endothelial cells but also produced mature vessels that included smooth muscle cells. (ahajournals.org)
  • The initial work, published in the JCI in 2000, described the method enabling successful attainment of blood outgrowth endothelial cells (BOEC). (jci.org)
  • cancer
  • Doctors evaluate the results of various blood tests to confirm that new blood cells are being produced and that the cancer has not returned. (stemcellcordblood.net)
  • Antibodies
  • B cell immune responses are measured by analyzing antibodies produced through the humoral immune response. (wikipedia.org)
  • However, there have been reports that elevated levels of IgM antibodies, altered T-cell function and/or proportionality of CD4:CD8 T cells may be associated with increased risk of GLILD, and GLILD has also been associated with specific genetic mutations in CVID, including CTLA-4 deficiency. (wikipedia.org)
  • organs
  • Here we report a novel mouse model that allows long-term single cell imaging of many organs. (jove.com)
  • The continued storage of GAGs in cells can lead to organs being affected in important ways. (wikipedia.org)
  • quantitative
  • Using the ratio ρ = L / M {\displaystyle \rho =L/M} of lymphoid (L) to myeloid (M) cells in blood as a quantitative marker, the stem cell compartment can be split into three categories of HSC. (wikipedia.org)
  • Together with his colleague, Dr. J.E. Till, McCulloch created the first quantitative, clonal method to identify stem cells and used this technique for pioneering studies on stem cells. (wikipedia.org)
  • cancerous
  • The cancerous T cells in the body initially migrate to the skin, causing various lesions to appear. (wikipedia.org)
  • In some individuals, the disease comes to light only after the cancerous cells overwhelm the bone marrow resulting in anemia producing tiredness or weakness. (wikipedia.org)