TransplantationProliferationRetinoic acidHematopoiesisSelf-renewalMacrophageHSCsMicePluripotentProgenitorsEmbryonicReceptorATRAPopulationsMutationTherapyLeukemiaEndothelial cellsMacrophagesLeukemiasAssayTumorApoptosisFunctionallyMultipotentAssaysGenesLineageDifferentiateVitroStromalSomatic cellsMesenchymal stemDiseaseBoneRegenerativeGene ExpressionMechanismsEnhancerAdultTumorsPathwaySclerosisTissuesCulturesBiologyLeukaemiaVivoPrognosisFormation of blooCharacterizationMyeloid progenitor cellCancerLineagesLentiviral
Transplantation11
- Hematopoietic Stem Cell Transplantation in Refractory Crohn's Disease: Should It Be Considered? (mdpi.com)
- Background Thalidomide is active in multiple myeloma and is associated with minimal myelosuppression, making it a good candidate for induction therapy prior to high-dose therapy with autologous stem-cell transplantation. (haematologica.org)
- High-dose therapy (HDT) combined with autologous stem-cell transplantation (ASCT) provides superior response and survival outcomes versus standard chemotherapy in patients with newly diagnosed (ND) multiple myeloma (MM). 1 , 2 Standard induction chemotherapy regimens, however, are often associated with hematologic stem-cell toxicity, which may compromise the collection of stem cells for ASCT. (haematologica.org)
- Background: In our country, transplantation centers differ in the age limit for allogeneic hematopoietic transplantation (ALOHT). (scielo.org)
- Autologous HSCT Improves Long-Term Survival Patients with rapidly progressive systemic sclerosis could find long-term mortality benefits from autologous hematopoietic stem cell transplantation. (medscape.com)
- Others can be cured with stem cell transplantation. (hematology.org)
- Findings published in JAMA Network Open suggested that voriconazole (Vfend) may be the best prophylaxis option for patients undergoing hematopoietic stem cell transplantation (HSCT), and posaconazole (Noxafil) may be the best prophylaxis option for patients with acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS). (cancernetwork.com)
- Wang J, Zhou M, Xu J, Zhou R, Chen B, Wan Y. Comparison of Antifungal Prophylaxis Drugs in Patients With Hematological Disease or Undergoing Hematopoietic Stem Cell Transplantation. (cancernetwork.com)
- Non-low-risk patients are evaluated for stem cell transplantation in first remission. (medscape.com)
- Treatment includes induction chemotherapy to achieve remission and postremission chemotherapy (with or without stem cell transplantation) to avoid relapse. (msdmanuals.com)
- The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
Proliferation6
- In a series of assays, they found that individuals with this variant had decreased TET2 expression and that the reduction of TET2 activity was linked to increased self-renewal and proliferation of hematopoietic stem cells. (genomeweb.com)
- DHX15 silencing greatly inhibited leukemia cell proliferation and induced cell apoptosis and G1-phase arrest. (oncotarget.com)
- Second, the rapid proliferation of these cells, along with a reduction in their ability to undergo programmed cell death (apoptosis), results in their accumulation in the bone marrow, the blood, and, frequently, the spleen and liver. (medscape.com)
- In acute myeloid leukemia (AML), malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived myeloid progenitor cell results in high circulating numbers of immature blood cells and replacement of normal marrow by malignant cells. (msdmanuals.com)
- Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)
- ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
Retinoic acid7
- Historically, acute promyelocytic leukemia (APL) was considered to be one of the most fatal forms of acute leukemia with poor outcomes before the introduction of the vitamin A derivative all- trans retinoic acid (ATRA). (nature.com)
- In the past two decades, therapy for newly diagnosed APL has evolved from an all- trans retinoic acid (ATRA)+chemotherapy backbone for all patients to the addition of arsenic trioxide (ATO) to ATRA with omission of chemotherapy in low-risk patients as a new standard of care. (nature.com)
- Describe the unique features of acute promyelocytic leukemia (AML M3), including the morphologic appearance of the promyelocyte, hemorrhagic complications, chromosomal abnormality, and treatment with induction therapy and all trans retinoic acid. (hematology.org)
- We found a significantly lower expression of key autophagy- (ATG-) related genes in primary AML as compared to healthy granulocytes, an increased autophagic activity during all- trans retinoic acid- (ATRA-) induced neutrophil differentiation, and an impaired AML differentiation upon inhibition of ATG3, ATG4D, and ATG5. (hindawi.com)
- The introduction of all-trans retinoic acid (ATRA) and, more recently, arsenic trioxide (ATO) into the therapy of acute promyelocytic leukemia (APL) has revolutionized the management and outcome of this disease. (nih.gov)
- Analyses on the expression of various stem cell-specific markers as well as experiments with all-trans-retinoic acid (ATRA), which has a proliferating effect on stem cells, moreover confirmed the presumed stem cell-like character of the CD4-expressing cells. (uni-goettingen.de)
- Patients with acute promyelocytic leukemia (APL) are treated according to a separate protocol (included in the care program) based on all-trans retinoic acid (ATRA) and arsenic trioxide (ATO). (cancercentrum.se)
Hematopoiesis9
- Overall, we observe that germline genetic variation shapes hematopoietic stem cell function, leading to CHIP through mechanisms that are specific to clonal hematopoiesis as well as shared mechanisms that lead to somatic mutations across tissues," co-senior authors Sekar Kathiresan and Pradeep Natarajan, both of the Broad, and their colleagues wrote in their paper. (genomeweb.com)
- The first wave, defined as primitive hematopoiesis, takes place in the extraembryonic yolk sac and generates transitory hematopoietic cell populations consisting of primitive erythrocytes. (amegroups.org)
- The second wave, referred to as definitive hematopoiesis, temporarily occurs in the fetal liver and transitions to the bone marrow and thymus, where multipotent hematopoietic stem cells (HSCs) contribute to the generation of all blood lineages ( 1 - 3 ). (amegroups.org)
- In zebrafish, primitive hematopoiesis occurs at early somite stages in the intermediate cell mass (ICM) and the rostral blood island (RBI). (amegroups.org)
- Despite these apparent differences in definitive hematopoiesis between fish and mammals, the definitive HSC niche functions to actively recruit stem cells and maintain their plasticity throughout adulthood ( 2 , 4 , 5 ). (amegroups.org)
- Figure 1 VCAM-1 + macrophages guide hematopoietic stem/progenitor cell to sites of definitive hematopoiesis. (amegroups.org)
- Subset of macrophages expressing ICAM-1 migrate to the sites of definitive hematopoiesis in AGM and CHT to recruit hematopoietic stem cells and initiate neonatal hematopoiesis and guide them further to sites of adult and definitive hematopoiesis in kidneys and thymus. (amegroups.org)
- Wnt signaling increases hematopoietic stem cell self-renewal and is activated in both myeloid and lymphoid malignancies, indicating involvement in both normal and malignant hematopoiesis. (ox.ac.uk)
- Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
Self-renewal7
- They further traced that locus to a likely causal variant that affects a TET2 enhancer to lead to an increase in the self-renewal of hematopoietic stem cells. (genomeweb.com)
- It is a heterogeneous disease, cellularly and molecularly, with an overarching theme of ectopic self-renewal and arrested differentiation potential at multiple levels of the hematopoietic tree ( 1 ). (frontiersin.org)
- Akin to the normal hematopoietic system, leukemias are sustained by a small number of leukemia stem-like cells (LSC), which can be distinct from the normal hematopoietic stem cells (HSC) but also exhibit functional characteristics of self-renewal and (abnormal or hindered) differentiation, and are often quiescent ( 2 - 4 ). (frontiersin.org)
- Autophagy is an intracellular degradation system that ensures a dynamic recycling of a variety of building blocks required for self-renewal, homeostasis, and cell survival under stress. (hindawi.com)
- The limbal niche supports the self-renewal of limbal epithelial stem cells (LESCs). (qxmd.com)
- To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
- Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)
Macrophage2
- Malignant lymphoma composed of large B lymphoid cells whose nuclear size can exceed normal macrophage nuclei, or more than twice the size of a normal lymphocyte. (lookformedical.com)
- Osteoclasts are bone-resorbing cells of monocyte/macrophage origin and are culprits of bone destruction associated with osteoporosis, rheumatoid arthritis, and cancer bone metastasis. (koreascience.kr)
HSCs4
- These HSCs are primarily derived from endothelial cells within the ventral wall of the dorsal aorta. (amegroups.org)
- The definitive HSCs in mammals first migrate to and repopulate in the fetal liver and then home to the bone marrow, while the definitive HSCs in zebrafish migrate first to the caudal hematopoietic tissue and then to their definitive anatomical sites in the kidney and thymus ( 5 - 8 ). (amegroups.org)
- Haematopoietic stem cells (HSCs) represent the best-characterized example of this phenomenon, but the molecular mechanisms responsible for their formation remain obscure. (ox.ac.uk)
- The stem cell leukaemia (SCL) gene encodes a basic helix-loop-helix (bHLH) transcription factor with an essential role in specifying HSCs. (ox.ac.uk)
Mice5
- Preliminary work has shown that mice with overactivation of the Hh pathway in CD4-expressing cells (Ptch flox/flox-CD4Cre mice) unexpectedly develop BCC after chemical carcinogenesis using the 7,12-dimethylbenzo(a)anthracene/12-O-tetradecanoylphorbol-13-acetate (DMBA/TPA) protocol. (uni-goettingen.de)
- In this process, flow cytometric analysis showed that CD4+CD49f+Sca-1+ cells are also expressed in immunodeficient NOD SCID and Rag2 mice. (uni-goettingen.de)
- The results showed that topical tamoxifen application in the R26-tdTomato-CD4CreERT2 mouse model does not result in sufficient in vivo labeling of target cells, but alternatively R26-tdTomato-CD4Cre mice are well suited for these studies. (uni-goettingen.de)
- One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
- Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
Pluripotent11
- Induced pluripotent stem cells (iPSC) express genes associated with pluripotency, have the capacity for infinite expansion, and can differentiate into cells from all three germ layers. (biomedcentral.com)
- Given the tremendous promise of regenerative medicine to enhance human health and treat disease, Congress included a provision in the 21st Century Cures Act to support a Regenerative Medicine Innovation Project ($30 million distributed over FY17 through FY20) for the funding of clinical research to further the field of regenerative medicine (RM) using adult stem cells, including autologous, non-autologous use as well as eligible induced pluripotent stem cells (iPSC). (nih.gov)
- According to a simplified model of this theory a newly-conceived human embryo consists of pluripotent stem cells (Type A), ones that can potentially divide into any body cells. (anti-agingfirewalls.com)
- Efficient generation of nonhuman primate induced pluripotent stem cells. (stemcell.com)
- Induced pluripotent stem (iPS) cells have great potential for regenerative medicine and gene therapy. (stemcell.com)
- In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
- Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
- This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
- Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
- However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
- These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)
Progenitors4
- 02:14 progenitors that make red blood cells. (yale.edu)
- In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
- Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
- Loss of hematopoietic stem cell function was associated with decreased expression of Cdkn1a (encoding the cell cycle inhibitor p21(cdk)), Sfpi1, Hoxb4 and Bmi1 (encoding the transcription factors PU.1, HoxB4 and Bmi-1, respectively) and altered integrin expression in Lin(-)Sca-1(+)c-Kit(+) cells, whereas PU.1 was upregulated in erythroid progenitors. (ox.ac.uk)
Embryonic2
- We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
- We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Receptor10
- Interleukin-6 on target cells binds to a membrane bound Interleukin-6 receptor. (wikipedia.org)
- Gp130 is expressed on all cells of the human body whereas the Interleukin-6 receptor is only found on few cells such as hepatocytes and some leukocytes. (wikipedia.org)
- Therefore, cells, which only express gp130 but no Interleukin-6 receptor are not responsive to Interleukin-6. (wikipedia.org)
- It was found, however, that the membrane-bound Interleukin-6 receptor can be cleaved from the cell membrane generating a soluble Interleukin-6 receptor. (wikipedia.org)
- The soluble Interleukin-6 receptor can bind the ligand Interleukin-6 with similar affinity as the membrane-bound Interleukin-6 receptor and the complex of Interleukin-6 and the soluble Interleukin-6 receptor can bind to gp130 on cells, which only express gp130 but no Interleukin-6 receptor. (wikipedia.org)
- The mode of signaling via the soluble Interleukin-6 receptor has been named Interleukin-6 trans-signaling whereas Interleukin-6 signaling via the membrane-bound Interleukin-6 receptor is referred to as Interleukin-6 classic signaling. (wikipedia.org)
- Therefore, the generation of the soluble Interleukin-6 receptor enables cells to respond to Interleukin-6, which in the absence of soluble Interleukin-6 receptor would be completely unresponsive to the cytokine. (wikipedia.org)
- Cells, which respond to Interleukin-6 alone do express an Interleukin-6 receptor whereas cells, which only respond to Hyper-IL-6 but not to Interleukin-6 alone depend in their response to the cytokine on Interleukin-6 trans-signaling. (wikipedia.org)
- The MRD levels were monitored by detection of clone-specific rearrangements of the immunoglobulin and T-cell receptor genes applying a quantitative polymerase chain reaction-based technique. (bvsalud.org)
- In addition, the receptor required for cell entry of the human immunodeficiency virus (HIV), CXCR4, was detected on human CD4+CD49f+CD29+ epidermal cells, suggesting a possible reservoir function of CD4+ keratinocytes for HIV. (uni-goettingen.de)
ATRA1
- Analysen zur Expression verschiedener stammzellspezifischer Marker sowie Experimente mit der auf Stammzellen proliferierend wirkenden all trans-Retinsäure (ATRA) bestätigten darüber hinaus den vermuteten stammzellartigen Charakter der CD4-exprimierenden Zellen. (uni-goettingen.de)
Populations5
- A classification of B-lymphocytes based on structurally or functionally different populations of cells. (lookformedical.com)
- Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
- Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
- Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
- To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
Mutation2
- Griscelli syndrome type 2 (GS-2) is a rare, autosomal recessive immune deficiency syndrome caused by a mutation in the RAB27A gene, which results in the absence of a protein involved in vesicle trafficking and consequent loss of function of in particular cytotoxic T and NK cells. (biomedcentral.com)
- All GS-2 iPSC clones displayed a normal karyotype (46XX or 46XY) and were shown to express the same RAB27A gene mutation that was present in the original somatic donor cells. (biomedcentral.com)
Therapy4
- Hematopoietic Stem Cell Gene Therapy for Cystinosis: From Bench-to-Bedside. (nih.gov)
- Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. (lookformedical.com)
- Chronic myeloid leukaemia is a hematopoietic stem cells disorder treated by tyrosine kinase inhibitors but often cause recurrence of the leukaemia after cessation of therapy, hence require alternative treatment. (bvsalud.org)
- This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
Leukemia13
- While PAX5 is an important tumor suppressor gene in B-cell acute lymphoblastic leukemia (B-ALL), it is also involved in oncogenic translocations coding for diverse PAX5 fusion proteins. (bvsalud.org)
- Leukemia is a group of clonal diseases derived from a single cell with a genetic alteration in bone marrow or peripheral lymphoid tissue, and each type is determined by the specificity of the source cell. (sld.cu)
- Acute myeloid leukemia is a hematopoietic neoplasm of dismal prognosis that results from the accumulation of immature myeloid blasts in the bone marrow and the peripheral blood. (frontiersin.org)
- DHX15 was highly expressed in hematopoietic stem cells and leukemia cells but was lowly expressed in mature blood cells. (oncotarget.com)
- DHX15 was down-regulated when AML patients achieved disease remission or when leukemia cell lines were induced to differentiate. (oncotarget.com)
- Knockdown of DHX15 inhibited the nuclear translocation and activation of the NF-kB subunit P65 in leukemia cells. (oncotarget.com)
- Describe the predominant cell types seen in the peripheral blood and/or bone marrow in each of the four major categories of leukemia (AML, ALL, CML, CLL). (hematology.org)
- We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)
- This study was conducted to investigate the effect of magnetite nanoparticles (MNPs) and magnetite folate core shell (MFCS) on leukemic and hepatocarcinoma cell cultures as well as their effect on the animal model of acute myelocytic leukemia (AML). (bvsalud.org)
- Invivo study, nanoparticles were evaluated to inspect their cytotoxic activity against SNU-182 (human hepatocellular carcinoma), K562 (human leukemia), and THLE2 (human normal epithelial liver) cells via MTT test. (bvsalud.org)
- Overexpression of FLI1 in erythroblasts causes inhibition of differentiation and ultimately the development of pre-T cell lymphoblastic leukemia/lymphoma [ 11 ]. (biomedcentral.com)
- Acute myelogenous leukemia (AML) is a malignant disease of the bone marrow in which hematopoietic precursors are arrested in an early stage of development. (medscape.com)
- Acute myeloid leukemia (AML) is a disease with diverse genetic features of the leukemic cells and with variable outcome. (cancercentrum.se)
Endothelial cells2
- It turned out that hematopoietic stem cells, neural cells, smooth muscle cells and endothelial cells are typical target cells of Interleukin-6 trans-signaling. (wikipedia.org)
- These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
Macrophages3
- During this first wave, small number of myeloid cells (e.g., primitive monocytes/macrophages and megakaryocytes) are also generated. (amegroups.org)
- and the RBI cells are derived from the lateral mesoderm of the zebrafish head and differentiate into macrophages ( 4 - 8 ). (amegroups.org)
- that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
Leukemias1
- The acute leukemias are aggressive malignancies that originate in a hematopoietic stem cell and are rapidly fatal without immediate treatment. (hematology.org)
Assay7
- In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)
- Differentiation capacity into cells from all three germ layers was confirmed using the teratoma assay. (biomedcentral.com)
- 4 xCELLigence real-time cell analysis 6 Diverse cancer immunotherapy applications 6 How does the xCELLigence real-time cell analysis assay work? (technologynetworks.com)
- While many methods have been developed to screen and evaluate the efficacy of immune cell‑mediated killing, there is a need for a more robust in vitro assay to accurately predict the in vivo behavior of therapies. (technologynetworks.com)
- The most commonly used method for measuring immune cell‑mediated killing is the release assay, where effector cell‑mediated disruption of the target cell membrane results in leakage of its cytoplasmic contents into the culture medium. (technologynetworks.com)
- Using a CRISPR Cas9-guided immunoprecipitation assay, we identify a circular RNA in the FLI1 promoter chromatin complex, consisting of FLI1 exons 4-2-3, referred to as FECR1.Overexpression of FECR1 enhances invasiveness of MDA-MB231 breast cancer cells. (biomedcentral.com)
- Using this CasIP assay, we identified FECR1, a FLI1 exonic circular RNA that binds to the FLI1 promoter and epigenetically activates FLI1 in breast cancer cells. (biomedcentral.com)
Tumor5
- B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. (lookformedical.com)
- If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. (lookformedical.com)
- This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. (lookformedical.com)
- Ewing sarcoma is a small round-cell tumor arising from mesenchymal cells. (blogspot.com)
- Basal cell carcinoma (BCC) is the most common tumor entity in human skin. (uni-goettingen.de)
Apoptosis4
- Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. (lookformedical.com)
- As Type D cells die from trauma or apoptosis they are replaced by new cells resulting from differentiation of Type B and Type C cells. (anti-agingfirewalls.com)
- In contrast, the restoration of DHX15 expression rescued cell viability and reduced cell apoptosis. (oncotarget.com)
- overexpression of DHX15 caused dramatic resistance to ATO-induced cell apoptosis, suggesting an important role for DHX15 in cell apoptosis. (oncotarget.com)
Functionally1
- 03:06 cell functionally in FINA? (yale.edu)
Multipotent1
- With growth, these proliferate and, in a remarkably articulated manner, progressively differentiate into multipotent stem cells (Type B), progenitor cells (Type C), mature body somatic cells (Type E), and many eventually become senescent cells (Type E). (anti-agingfirewalls.com)
Assays3
- While the data provided by these assays help piece together an understanding of different facets of immune cell‑mediated killing, it is important to note that the parameters being reported often do not correlate with target cell killing efficacy in vivo. (technologynetworks.com)
- This study determines the anti-cancer effect of leaf, root and bulb methanolic and aqueous extracts of B. natalensis and C. comosum in chronic human myelogenous leukaemia (K562) cell line by MTT, Hoechst bis-benzimide nuclear and annexin V stain assays. (bvsalud.org)
- 35 mm culture dishes, pre-tested for optimal colony growth without supporting anchorage-dependent cells in methylcellulose-based assays. (stemcell.com)
Genes4
- The majority of mantle-cell lymphomas are associated with a t(11;14) translocation resulting in overexpression of the CYCLIN D1 gene (GENES, BCL-1). (lookformedical.com)
- While PU.1-dependent induction of myeloid-specific target genes has been intensively studied, negative regulation of stem cell or alternate lineage programs remains incompletely characterized. (zfin.org)
- These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)
- explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
Lineage5
- GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)
- Furthermore, in order to provide a suitable mouse model for future experiments, studies were performed on two lineage tracing mouse models in which the progeny of CD4-expressing cells are labeled with the red fluorescent tdTomato protein. (uni-goettingen.de)
- The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
- This requirement strongly limits the number of solutions or entiation and lineage-specification, programmed cell death, and ``states'' for the system. (lu.se)
- 2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
Differentiate5
- The above list is in order of increasing cell-type specificity and decreasing cell-type potency to differentiate into other cell types. (anti-agingfirewalls.com)
- Starting at conception and throughout life, all cells on this list except the senescent ones will selectively reproduce and possibly differentiate into cells of types further down in the list. (anti-agingfirewalls.com)
- At maturity there are relatively very few Type A cells and a mix of Type B, C and D cells, Type B and C cells typically live in protected stem cell niches where they reproduce and, as-needed differentiate to become the normal working body Type D cells. (anti-agingfirewalls.com)
- At an advanced age, the pools of Type B and Type C cells become depleted in part because of replicative senescence and the cells remaining in the pools lose their ability to differentiate as necessary to replace Type D cells. (anti-agingfirewalls.com)
- Cells in those pools replicate and differentiate throughout life. (anti-agingfirewalls.com)
Vitro4
- 02:47 and red blood cells in vitro. (yale.edu)
- In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
- Cells grown in vitro from neoplastic tissue. (lookformedical.com)
- The results of in vitro C3 uptake studies and the effect of IVIG on the clearance of preopsonized cells suggest that IVIG produces a kinetic depression of C3 uptake and modifies the process of complement fragment deposition on erythrocytes. (medscape.com)
Stromal3
- MyeloCult™ media have been developed for the long-term culture of hematopoietic progenitor cells in association with adherent stromal cells. (stemcell.com)
- Stromal cell feeder layers (e.g. (stemcell.com)
- The corneal stromal stem cell (CSSC) is an important component in the niche that regulates the LESC phenotype. (qxmd.com)
Somatic cells1
- In essence, early-on the body sets up pools of stem and progenitor cells to replace lost somatic cells. (anti-agingfirewalls.com)
Mesenchymal stem3
- In this review, we introduce the broad application of β-TCP in tissue engineering and discuss the different approaches that β-TCP scaffolds are customized, including physical modification (e.g., pore size, porosity and roughness) and the incorporation of metal ions, other materials (e.g., bioactive glass) and stem cells (e.g., mesenchymal stem cells). (frontiersin.org)
- We subsequently discuss how β-TCP can regulate osteogenic processes to aid bone repair/healing, namely osteogenic differentiation of mesenchymal stem cells, formation of blood vessels, release of angiogenic growth factors, and blood clot formation. (frontiersin.org)
- The main challenge for large bone defect repair and regeneration remains the inadequate recruitment of mesenchymal stem cells (MSCs), reduced vascularization, and decreased growth factors stimulation within the scaffold construct to support cell viability and tissue growth. (frontiersin.org)
Disease5
- These cells can be used in future studies for the development of novel treatment options and to study the pathophysiology of GS-2 disease. (biomedcentral.com)
- As people age, they can acquire somatic mutations that lead to clonal expansion within their population of regenerating hematopoietic stem cells, which in turn can lead to hematological cancer and coronary heart disease. (genomeweb.com)
- The only common feature among these tumors is the absence of giant REED-STERNBERG CELLS, a characteristic of Hodgkin's disease. (lookformedical.com)
- Many Type D cells senesce and become Type E cells which make the corresponding organs shrivel and be susceptible to cancers and other disease processes. (anti-agingfirewalls.com)
- Name at least three disease features which result from replacement of marrow cells with myeloblasts or circulation of myeloblasts. (hematology.org)
Bone6
- Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
- The underlying pathophysiology in AML consists of a maturational arrest of bone marrow cells in the earliest stages of development. (medscape.com)
- Patients with AML present with symptoms resulting from bone marrow failure, symptoms resulting from organ infiltration with leukemic cells, or both. (medscape.com)
- Storage of bone marrow or blood leukemic cells in biobanks is strongly recommended. (cancercentrum.se)
- Bone marrow aspiration is performed after hematopoietic recovery, or day 28 at the latest. (cancercentrum.se)
- 15]. The percentage of bone common of which are trisomy 8, mono- marrow blast cells for estimation of the Myelodysplastic syndrome (MDS) is somy 7 and 5q- [11]. (who.int)
Regenerative3
- Interleukin-6 trans-signaling mediates the pro-inflammatory activities of Interleukin-6 whereas Interleukin-6 classic signaling governs the protective and regenerative Interleukin-6 activities. (wikipedia.org)
- Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
- They may also serve as an unlimited source of therapeutically active cells for regenerative medi- cine. (lu.se)
Gene Expression3
- Stem cell gene expression evolves with age. (anti-agingfirewalls.com)
- Although in principle stem cells can replicate indefinitely, in fact they age as the organism ages, continuing to change their gene expression. (anti-agingfirewalls.com)
- Cell cycle gene expression networks discovered using systems biology: Significance in carcinogenesis. (genenetwork.org)
Mechanisms2
- The pathogenic mechanisms involve a wide range of cells and soluble factors. (mdpi.com)
- One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). (lookformedical.com)
Enhancer2
- The researchers homed in on a set of about two dozen likely causal variants, which they hypothesized affects a TET2 enhancer in hematopoietic stem cells. (genomeweb.com)
- Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors. (ox.ac.uk)
Adult5
- Life depends on constant replenishment of human body cells with new cells created by differentiation of adult stem cells. (anti-agingfirewalls.com)
- IF we could find a way to rejuvenate adult stem cells in their niches, then the stem cell supply chain could possibly be transformed from being a once-through-in-life process to a continuing closed-loop process. (anti-agingfirewalls.com)
- GnRHa and Testosterone Effect on Adult Height in Trans Boys A new study examines the effects of testosterone and GnRH treatment on adult height among transgender boys. (medscape.com)
- 2001. Subchronic dermal application of N,N-diethyl m-toluamide (DEET) and permethrin to adult rats, alone or in combination, causes diffuse neuronal cell death and cytoskeletal abnormalities in the cerebral cortex and the hippocampus, and purkinje neuron loss in the cerebellum. (cdc.gov)
- Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
Tumors2
- A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes. (lookformedical.com)
- B-cell lymphoid tumors that occur in association with AIDS. (lookformedical.com)
Pathway1
- Activation of the canonical Wnt pathway leads to loss of hematopoietic stem cell repopulation and multilineage differentiation block. (ox.ac.uk)
Sclerosis1
- Hypochromic Red Cells and Survival in Systemic Sclerosis What could the presence of hypochromic red cells tell us about survival in patients with systemic sclerosis? (medscape.com)
Tissues3
- The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
- hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
- genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
Cultures1
- A cytotoxic effect of MNPs and MFCS was detected in previous cell cultures. (bvsalud.org)
Biology1
- Stem cells are a central feature of metazoan biology. (ox.ac.uk)
Leukaemia1
- Rarely, immunophenotypically immature B-cell precursor acute lymphoblastic leukaemia (BCP-ALL) carries an immunoglobulin-MYC rearrangement (IG-MYC-r). (bvsalud.org)
Vivo1
- The Hyper-IL-6 protein has also been used to explore the physiologic role of Interleukin-6 trans-signaling in vivo. (wikipedia.org)
Prognosis1
- All patients can evolve into trisomy 8+ MDS [12], cells, since acquiring adequate samples diagnosed with MDS have a reduced in which 8+ appears to confer a favour- for flow cytometric analysis renders the life expectancy compared with age- able prognosis [13]. (who.int)
Formation of bloo1
- Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
Characterization1
- In summary, the results of this work provide an important basis for further characterization of CD4+ keratinocytes using suitable mouse models and cell cultivation methods. (uni-goettingen.de)
Myeloid progenitor cell1
Cancer5
- Recently, in breast cancer patients, it was shown with the help of Hyper-IL-6 that IL-6 trans-signaling via phosphoinositid-3-kinase signaling activates disseminated cancer cells long before metastases are formed. (wikipedia.org)
- Cancer immunotherapies can be divided into four major categories: - Cytokines/immunomodulation agents - Monoclonal antibodies - Cell‑based therapies - Oncolytic viruses Though monoclonal antibodies currently represent the largest class of commercialized cancer immunotherapies, cell‑based therapies are rapidly making headway. (technologynetworks.com)
- This class of personalized therapies involves collecting immune cells from an individual, engineering them to recognize and kill cancer cells, before culturing, and reintroducing them into the same individual. (technologynetworks.com)
- While the importance of autophagy for cell homeostasis and survival has long been appreciated, its role in tumorigenesis and cancer progression is still developing [ 10 , 11 ]. (hindawi.com)
- In non-small cell lung cancer, the FLI1 expression score is associated with the stage of SCLC. (biomedcentral.com)
Lineages1
- The differentiation of hematopoietic stem cells (HSC) into myeloid lineages requires the transcription factor PU.1. (zfin.org)
Lentiviral1
- We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)