• 2 Evaluation of harvest adequacy requires the use of reliable progenitor cell assays and this is usually achieved by CD34 cell counting using flow cytometry. (bdbiosciences.com)
  • Flow cytometric applications for CD34+ cell identification and enumeration provide a rapid, quantitative and reproducible method to evaluate the progenitor cell population. (bdbiosciences.com)
  • We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)
  • The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
  • The various mature blood cell types are all ultimately derived from a single class of progenitor cell known as hematopoietic stem cells. (justia.com)
  • Although bone marrow fibrosis is seen in a variety of malignant and non-malignant disease states, the deposition of reticulin and collagen fibrosis in the bone marrow of patients with myelofibrosis is believed to be mediated by the myelofibrosis hematopoietic stem/progenitor cell, contributing to an impaired microenvironment favoring malignant over normal hematopoiesis. (haematologica.org)
  • We discuss the rationale of various anti-fibrogenic treatment strategies targeting the clonal hematopoietic stem/progenitor cell, aberrant signaling pathways, fibrogenic cytokines, and the tumor microenvironment. (haematologica.org)
  • 1 Neural stem/progenitor cell proliferation and differentiation: role of sonic hedgehog and wingless/int-1 proteins. (vinastemcelllab.com)
  • No obvious correlations between clinical parameters, including age, karyotype, existence of paroxysmal nocturnal hemoglobinuria clones, pretreatment blood counts, progenitor cell counts, and the response to immunosuppressive therapy (IST), were found. (springermedizin.at)
  • The researchers used whole-exome sequencing to detect the presence of clonal hematopoiesis of indeterminate potential in peripheral blood cells and associated that discovery with coronary heart disease using samples from 4 case-control studies that together enrolled 4,726 participants with coronary heart disease and 3,529 controls. (ascopost.com)
  • Depending on the degree of bone marrow damage (i.e., suppression), patients who undergo cytoreductive therapy must also receive therapy to reconstitute bone marrow function (hematopoiesis). (justia.com)
  • Inhibition of hematopoiesis results in reduced populations of blood forming cells. (hoajonline.com)
  • Hematopoiesis is the process of blood cell formation. (thenoveldifference.com)
  • gene transfer, hematopoietic stem cells, lentiviral vectors, mesenchymal stem/progenitor cells, bone marrow transplantation, hematopoiesis Introduction HIV-based lentiviral vectors (LV) were proven to be capable of transducing a broad spectrum of nondividing cells in multiple mammalian species cIAP1 Ligand-Linker Conjugates 14 [1]. (eyesoftheelephants.com)
  • 4 Maintenance of hematopoiesis: role of early b cell factor 2 matthias kieslinger. (vinastemcelllab.com)
  • WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
  • We hypothesize that engagement of E-selectin ligands on human acute leukemia cells programs efficient BM metastasis and also enables niche lodgment, serving to displace resident HSPCs from their proper growth microenvironment and thereby promoting leukemic cell proliferation. (cancer.gov)
  • While progenitor cells can divide and replicate, their proliferation potential is more restricted compared to stem cells. (thenoveldifference.com)
  • A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. (curehunter.com)
  • PMF is characterized by proliferation predominantly of bone marrow megakaryocytes and granulocytes, resulting in deposition of fibrous connective tissue, and often associated with peripheral blood leukoerythroblastosis, cytopenias, and constitutional symptoms. (oncohemakey.com)
  • Acute lymphoblastic leukemia (ALL): Bone marrow shows proliferation of large and heterogeneous lymphoblasts consistent with pre-B-cell ALL (French-American-British L2 morphology). (medscape.com)
  • 13 Proliferation of bone marrow-derived human mesenchymal stem cells: role of enamel matrix proteins. (vinastemcelllab.com)
  • Second, the rapid proliferation of these cells, along with a reduction in their ability to undergo programmed cell death (apoptosis), results in their accumulation in the bone marrow, the blood, and, frequently, the spleen and liver. (medscape.com)
  • Under normal conditions the CXCR4 pathway regulates the hematopoietic stem cell niche in the bone marrow (BM)-a property that has led to the approval of the CXCR4 antagonist plerixafor together with G-CSF (AMD3100, Mozobil) for mobilization from the BM and collection of hematopoietic precursors from the blood for transplantation of myeloma and lymphoma patients. (biokine.com)
  • Are you considering a blood stem cell transplant? (nih.gov)
  • This study is investigating a new method for collecting blood stem cells from donors to see if it reduces transplant complications, such as rejection, in patients who have blood diseases. (nih.gov)
  • Have you received a stem cell transplant? (nih.gov)
  • This study aims to monitor the long-term health of patients who have received a donor stem cell transplant from the NIH Clinical Center. (nih.gov)
  • Are you scheduled for a stem cell transplant with cord blood? (nih.gov)
  • Dr. Abutalib is Assistant Director in the Stem Cell Transplant & Cell Therapy Program at Cancer Treatment Centers of America in Chicago. (ascopost.com)
  • The number of mononuclear cells collected, the patient's disease status at the time of transplant and the presence of circulating plasma cells in the harvested product did not have a significant impact on time to platelet engraftment. (elsevierpure.com)
  • This is an important study, and ultimately, I think our goal is to overcome the use of chemotherapy and radiation, which can destroy healthy cells along with malignant ones," commented Catherine Bollard, MD, MBChB, a bone and marrow transplant specialist at George Washington University/Children's National Medical Health System, Washington, DC. (medscape.com)
  • Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
  • In a recent issue of Nature , Fan Zhou, Bing Liu and colleagues present their work on tracing the developmental origin of blood stem cells, also known as hematopoietic stem cells (HSCs), in mice. (usc.edu)
  • Gene-modified, autologous bone marrow transplantation can circumvent the severe immunologic complications that occur when a related HLA-mismatched donor is used and thus represents an attractive alternative (see below). (medscape.com)
  • 2 HSCs are primarily found in bone marrow niches, but a small fraction of HSCs can also be found in the peripheral blood. (bdbiosciences.com)
  • 3 Following exogenous stimulation, such as chemotherapy or using growth factors such as granulocyte colony stimulating factor (G-CSF) and filgrastim, the number of HSCs in the peripheral blood increases, either becoming on par or even exceeding the number in the bone marrow. (bdbiosciences.com)
  • This pharmacologically induced egress of HSCs into peripheral blood, called mobilization, is utilized as the preferred strategy for generating HSCs for transplantation. (bdbiosciences.com)
  • 3 Autologous stem cell transplantation almost exclusively uses peripheral blood, while for allogeneic SCT, T-lymphocytes in the peripheral blood could pose some danger. (bdbiosciences.com)
  • Diagnosis requires demonstration of peripheral pancytopenia and a bone marrow biopsy revealing a hypocellular marrow. (merckmanuals.com)
  • Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
  • Specifically, methods are disclosed for mobilizing hematopoietic stem cells into a subject's peripheral blood. (justia.com)
  • In particular, embodiments of the method involve inhibition of both Rac1 and Rac2 GTPases to increase the numbers of hematopoietic stem cells into a subject's peripheral blood of a subject. (justia.com)
  • Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
  • In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
  • Hematopoietic stem cells (HSCs) are a type of multipotent stem cell found primarily in the bone marrow and, to a lesser extent, in peripheral blood. (thenoveldifference.com)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • Following intravenous administration of a first-generation LV into adult mice, we found that bone marrow exhibited the highest levels of transgene among nine organs examined, with more than 10% green fluorescent protein-positive (GFP+) cells detected in peripheral blood leukocytes (PBL) in these mice [9]. (eyesoftheelephants.com)
  • CD34, a transmembrane phosphoglycoprotein, is present on immature hematopoietic precursor cells and all hematopoietic colony-forming cells in bone marrow and blood, including unipotent and pluripotent progenitor cells. (bdbiosciences.com)
  • Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)
  • At first, the mesenchymal stem cell was thought to be the primary component of this undifferentiated cell type, however it is now evident that within the adipose extracellular matrix are also adipocytic precursors (known as progenitor cells) adherent to adipocytes, and in close approximation to a variety of additional undifferentiated multipotent and pluripotent cells, including pericytes and endothelial cells, all thought to play important roles in mesenchymal-stromal derived tissue regeneration. (journalofprolotherapy.com)
  • True stem cells are both pluripotent-that is they can give rise to all cell types-and capable of self-renewal. (justia.com)
  • The pluripotent stem cells (eg. (hoajonline.com)
  • Retention of cellular phenotype is the hallmark of differentiation of pluripotent stem cells. (hoajonline.com)
  • 6 Induced pluripotent stem cells from human extra-embryonic amnion cells: role of dna methylation in mainting stemness. (vinastemcelllab.com)
  • 12 Induced pluripotent stem cell production and characterization: an overview of somatic cell reprogramming. (vinastemcelllab.com)
  • 14 Pluripotent cell-derived glial precursor cells for the delivery of therapeutic proteins to the central nervous system. (vinastemcelllab.com)
  • Attention is focused on clonal myeloid disorders, acute lymphoblastic leukemia, lymphoma, and plasma cell dyscrasias. (ascopost.com)
  • The chronic myeloproliferative neoplasms (MPNs) are clonal disorders characterized by overproduction of mature myeloid cells. (oncohemakey.com)
  • The MPNs comprise a set of clonal HSC disorders, characterized by the overproduction of 1 or more mature myeloid cell types. (oncohemakey.com)
  • Acute lymphoblastic leukemia (acute lymphocytic leukemia, ALL) is a malignant (clonal) disease of the bone marrow in which early lymphoid precursors proliferate and replace the normal hematopoietic cells of the marrow. (medscape.com)
  • Although autologous hematopoietic cell transplantation (HCT) is an established therapy for patients with relapsed acute promyelocytic leukemia (APL) after returning to complete remission (CR), the role of allogeneic HCT remains unclear for treating relapsed APL. (bvsalud.org)
  • The specific effect on bone marrow fibrosis of JAK2 inhibition, and other rationally based therapies currently being evaluated in myelofibrosis, has yet to be fully elucidated. (haematologica.org)
  • 4 CD34 expression is historically related to hematopoietic cells and it is considered as the marker of HSCs. (bdbiosciences.com)
  • Flow cytometric enumeration of CD34+ HSCs and progenitor cells is an established method for the evaluation of bone marrow and stem cell grafts. (bdbiosciences.com)
  • Although human HSCs as vehicles to treat metachromatic leukodystrophy (MLD) has been used to treat patients with early onset MLD in a phase I/II trial, the HSCs give rise to all different blood cell lineages, such as the myeloid and lymphoid cell lineages [ 11 ]. (hindawi.com)
  • HSCs have the remarkable ability to differentiate into a wide range of specialized blood cells. (thenoveldifference.com)
  • Mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) were derived from syngenic mouse bone marrow. (vinastemcelllab.com)
  • Diabetic mice were transplanted with HSCs and insulin-producing cells (IPCs) via the tail vein after destroying the bone marrow. (vinastemcelllab.com)
  • The results showed that transplantation of both IPCs and HSCs elicited greater improvements in body weight, blood glucose level and survival time than did transplantation of HSCs or IPCs alone. (vinastemcelllab.com)
  • Hematopoietic stem cells give rise to progenitor cells, which, in turn, differentiate into specific blood cell lineages. (thenoveldifference.com)
  • At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
  • Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)
  • 6 Causes of early death include leukemic transformation, complications arising from progressive bone marrow failure, portal/pulmonary hypertension, infections, thrombosis and bleeding. (haematologica.org)
  • 2 Sensitivity of hematopoietic and leukemic stem cells to hoxa gene levels. (vinastemcelllab.com)
  • Patients with AML present with symptoms resulting from bone marrow failure, symptoms resulting from organ infiltration with leukemic cells, or both. (medscape.com)
  • Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
  • With high levels of platelet-derived growth factors and cytokines, this combination provides both a living bioscaffold and a multipotent cell replenishment source useful for enhanced musculoskeletal healing. (journalofprolotherapy.com)
  • Polyclonal T-cell reconstitution of X-SCID recipients after in utero transplantation of lymphoid-primed multipotent progenitors. (ox.ac.uk)
  • Herein, we demonstrate in a congenic setting that recently identified lymphoid-primed multipotent progenitors are superior to hematopoietic stem cells in providing rapid lymphoid reconstitution after IUHCT of X-SCID recipients, and sustain in the long-term B cells, polyclonal T cells, as well as short-lived B-cell progenitors and thymic T-cell precursors. (ox.ac.uk)
  • 3 Myelofibrosis (MF) refers to the Philadelphia chromosome ( BCR-ABL1 )-negative myeloproliferative neoplasm (MPN) originating at the level of the multipotent hematopoietic stem cell. (haematologica.org)
  • Recognition of the vast number of undifferentiated cells associated with the stromal vascular fraction has resulted in extensive research demonstrating the heterogeneity of such cells, and their ability to participate in production of all mesodermal-derived tissues. (journalofprolotherapy.com)
  • Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)
  • The subject's blood can be processed and used to repopulate the destroyed lymphohematopoietic system of a recipient and may in the future be utilized to repair a variety of non-hematopoietic tissues. (justia.com)
  • Progenitor cells are often located within specific tissues, close to where they will ultimately function. (thenoveldifference.com)
  • They offer the potential to replace damaged or dysfunctional cells in specific tissues or organs. (thenoveldifference.com)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • In addition cIAP1 Ligand-Linker Conjugates 14 to HSC, bone marrow contains mesenchymal stem/progenitor cells (MSC), which can differentiate into mature cells of multiple mesenchymal tissues cIAP1 Ligand-Linker Conjugates 14 including fat, bone, and cartilage. (eyesoftheelephants.com)
  • Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
  • Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)
  • Stem cell transplantation is performed after high-dose chemotherapy (HDC) to restore a cancer patient's blood and immune cell production capacity. (bdbiosciences.com)
  • After harvesting, the patient is given high dose chemotherapy or radiotherapy and the bone marrow function is reconstituted by infusion of the cells harvested earlier. (justia.com)
  • Mobilization human CD34+ cells for the purpose of donor (allogeneic) transplantation after high-dose chemotherapy is currently performed using a 4-5-day treatment cycle with G-CSF and a 1-2 day apheresis procedure. (biokine.com)
  • Evidence suggests that the CXC-chemokine receptor-4 (CXCR4) pathway plays a major role in cancer cell homing and metastasis and in shaping the tumor microenvironment (TME), and thus represents a potential target for cancer therapy. (biokine.com)
  • However, better understanding of the role of increased JAK-STAT signaling [either through activating mutations ( JAK2 , MPL515L/K ) within the signaling pathway, or mutations involving CALR ], the role of deregulated pro-inflammatory cytokine expression, and the impaired bone marrow microenvironment is transforming the treatment approach for MF. (haematologica.org)
  • In this setting, immediately perform a bone marrow aspiration and obtain a biopsy from the posterior iliac crest. (medscape.com)
  • The workup for AML includes blood tests, bone marrow aspiration and biopsy (the definitive diagnostic tests), and analysis of genetic abnormalities. (medscape.com)
  • Embryonic stem cells which are characteristic of totipotency differentiate into generations of the multiple organs. (hoajonline.com)
  • The totipotentstem cells differentiate further into pluripotency thatpopulate the organs with different sub-divisional components of the various individual organs. (hoajonline.com)
  • mesenchymal stem cells - placenta) differentiate into multipotency as precursors to formation of the cellular components. (hoajonline.com)
  • They differentiate into very specific cells. (thenoveldifference.com)
  • Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)
  • Studies have increasingly focused on the potential therapeutic effects of stem cell transplantation for neurological diseases [ 10 ]. (hindawi.com)
  • 1, 2 Recently, Prolotherapists have begun to utilize the potential of autologous adipose (fat)-derived stem/stromal cells (AD-SC) within non-manipulated fat graft scaffolding, combined with high-density PRP concentrates (HD-PRP) to provide a potent biological therapeutic combination. (journalofprolotherapy.com)
  • Therapeutic regeneration of molecularly engineered and transplanted autologous stem cells in neurological and cardiovascular diseases due to inability of self-regeneration or repair of endogenous stem cells at sites of inflammation or disease at various stages of progress for regeneration of the damaged tissue depending upon the sites and nature of degeneration. (hoajonline.com)
  • Cancer stem cellsand their susceptibility over their expected to be "downstream or progeny" cancer cells represent a target for therapeutic interventions to prevent latency and consequently relapse or metastasis of the malignant disease. (hoajonline.com)
  • Autologous hematopoietic stem cell transplantation and reconstitution: Different species and including primates and humans for studies are being used for development, localization, and therapeutic strategies. (hoajonline.com)
  • Progenitor cells are being explored for various therapeutic applications, including tissue engineering and regenerative medicine. (thenoveldifference.com)
  • Hematopoietic stem cell transplantation remains the only curative therapeutic approach that reliably results in resolution of bone marrow fibrosis in patients with myelofibrosis. (haematologica.org)
  • Recently, mesenchymal stromal cell (MSC)-derived small extracellular vesicles (EVs) have emerged as a potential new therapeutic agent for stroke treatment. (explorationpub.com)
  • The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
  • We further provide evidence for IUHCT of hematopoietic stem cells giving superior B- and T-cell reconstitution in fetal X-SCID recipients compared with neonatal and adolescent recipients. (ox.ac.uk)
  • We retrospectively analyzed Japanese nationwide transplantation registry data of patients with relapsed APL age ≥16 years who underwent allogeneic HCT between 2006 and 2020. (bvsalud.org)
  • In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
  • Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
  • Moreover, in human studies, JAK2V617F has been identified in the stem cell-enriched CD34 + CD38 − CD90 + Lin − compartment, common myeloid progenitors, granulocyte-monocyte precursors, and megakaryocyte-erythroid precursors as well as natural killer cells, B cells, and T cells in some patients, confirming its origin in an early hematopoietic progenitor. (oncohemakey.com)
  • The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. (hindawi.com)
  • When administered post-stroke, mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) induce neuroprotection, promote brain remodeling and plasticity, and enhance neurological recovery in rodents and non-human primates via mechanisms that involve immunomodulation and anti-inflammation. (explorationpub.com)
  • For many hematopoietic malignancies, collection and infusion of CD34+ hematopoietic stem/progenitor cells following chemotherapy is critical. (bdbiosciences.com)
  • They can also be used for increasing white blood cell survival and for chemotherapy. (justia.com)
  • This is defined by their ability to repopulate an individual whose hematopoietic system has been destroyed by radiation or chemotherapy. (justia.com)
  • The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
  • To participate in this study, you must have a disorder that compromises your body's ability to make blood cells. (nih.gov)
  • Neutropenia is an abnormally low concentration of neutrophils (a type of white blood cell) in the blood. (wikipedia.org)
  • Neutrophils make up the majority of circulating white blood cells and serve as the primary defense against infections by destroying bacteria, bacterial fragments and immunoglobulin-bound viruses in the blood. (wikipedia.org)
  • A common reference range for the absolute neutrophil count (ANC), which is calculated on the basis of the percentage of neutrophils in the white blood cell count (see the Absolute Neutrophil Count calculator) is 2500-8000/µL. (medscape.com)
  • Umbilical cord blood stem cell transplantation (UCBSCT) offers several advantages, including ready availability of the unit, a lower risk of transmitting viral diseases, no risk to the donor, and a lower risk of GVHD even in the absence of a perfect HLA match. (medscape.com)
  • In this proposal, using E-selectin binding assays under both static and fluid shear conditions, together with complementary techniques in flow cytometry and western blotting, we will analyze the E-selectin binding activity of leukemia cells isolated from blood and BM of patients with acute leukemias. (cancer.gov)
  • There is increasing evidence that these growth zones are created by the display of particular sugar molecules on the surface of blood cells that act like Velcro to anchor the cells to marrow vessels. (cancer.gov)
  • Overview of Decreased Erythropoiesis Anemia, a decrease in the number of red blood cells (RBCs), hemoglobin (Hb) content, or hematocrit (Hct), can result from decreased RBC production (erythropoiesis), increased RBC destruction. (merckmanuals.com)
  • Pure Red Blood Cell Aplasia Acquired pure red blood cell aplasia is a disorder of erythroid precursors that results in an isolated normocytic anemia. (merckmanuals.com)
  • Interestingly, grafts of single HSC precursor cells from 11-day-old mouse embryos showed quite different capacities to repopulate the blood. (usc.edu)
  • Sequential rounds of division and differentiation give rise to an enormous amplification of cell numbers, necessary for the production of mature blood cells. (justia.com)
  • These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
  • They are like giants, capable of turning into any blood cell in our body. (thenoveldifference.com)
  • These remarkable cells play a crucial role in the production and maintenance of the body's blood and immune cell supply. (thenoveldifference.com)
  • Hematopoietic stem cells have the unique capacity to continuously self-renew and maintain a pool of stem cells for the lifelong production of blood cells. (thenoveldifference.com)
  • This regenerative ability ensures a constant supply of blood cells throughout an individual's lifetime. (thenoveldifference.com)
  • Here, they reside in specialized niches where they remain quiescent until needed to replenish and repair the blood cell population. (thenoveldifference.com)
  • Hematopoietic stem cells are pivotal in maintaining the body's blood cell supply, and their versatility makes them invaluable in both medical treatments and scientific research related to blood disorders and regenerative medicine. (thenoveldifference.com)
  • They have already committed to a particular differentiation pathway, meaning they are destined to become a specific type of cell, such as a red blood cell, white blood cell, or platelet in the case of hematopoietic progenitor cells. (thenoveldifference.com)
  • For example, hematopoietic progenitor cells are found in the bone marrow, as this is where blood cell production occurs. (thenoveldifference.com)
  • Hematological malignancies are the kinds of cancer affecting blood, bone marrow and lymph nodes. (medgadget.com)
  • A malignancy in the lymphoid lineage that includes white blood cells such as T lymphocytes and B lymphocytes. (medgadget.com)
  • Myeloma cells produce excess abnormal proteins, called paraproteins, in the bone marrow that can cause your blood to become so thick that it flows sluggishly in your body, a condition called hyperviscosity. (webmd.com)
  • In PV, bone marrow panmyelosis is associated with increased numbers of red blood cells and, in some patients, neutrophilia and/or thrombocytosis. (oncohemakey.com)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • European Group for Blood and Marrow Transplantation Severe Aplastic Anaemia Working Party. (springermedizin.at)
  • Most AML subtypes are distinguished from other related blood disorders by the presence of more than 20% blasts in the bone marrow. (medscape.com)
  • First, the production of normal blood cells markedly decreases, which results in varying degrees of anemia , thrombocytopenia , and neutropenia . (medscape.com)
  • Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
  • It was also observed by others that a significant transgene signal was detected in the bone marrow (BM) by PCR analysis in adult mice of systemic administration of HIV-biased LV [10]. (eyesoftheelephants.com)
  • Recently, we exhibited detectable levels of transgene (up to 3.9%) in PBL of mice 4 months after secondary bone marrow transplantation (BMT) of HSC transduced by delivery of LV in newborn pups [11]. (eyesoftheelephants.com)
  • The BD ® Stem Cell Enumeration (SCE) Kit provides simultaneous enumeration of viable dual-positive CD45+/CD34+ hematopoietic stem cell populations in CD34+ absolute counts (cells/µL) as well as the percentage of the total viable leucocyte count that is CD34+ (%CD34). (bdbiosciences.com)
  • Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
  • 24 Proteomic characterization of mesenchymal stem cell-like populations derived from various tissue types. (vinastemcelllab.com)
  • Eligible patients were greater than 18 years of age with Ph- relapsed or refractory B-cell precursor ALL. (southcarolinablues.com)
  • This study aimed to investigate allogeneic HCT outcomes in patients with relapsed APL, focusing particularly on those who underwent transplantation in non-CR and those who had relapsed after prior autologous HCT. (bvsalud.org)
  • A total of 195 patients were eligible for this analysis, including 69 who underwent transplantation in non-CR and 55 who relapsed after prior autologous HCT. (bvsalud.org)
  • The 5-year overall survival (OS) rates for patients who underwent transplantation in CR and non-CR were 58% and 39%, respectively (P = .085), if they did not have a history of prior autologous HCT. (bvsalud.org)
  • Some patients experience relapse or have refractory disease, despite conventional cytotoxic chemotherapies and allogeneic transplantation, and a variety of new agents and treatment strategies have emerged. (bvsalud.org)
  • Allogeneic bone marrow transplantation has become the standard of care for certain patients with SCIDs (eg, X-linked severe combined immunodeficiency [XSCID], ADA deficiency). (medscape.com)
  • Bertrand et al reported on a European experience with 178 patients in 18 centers who were treated with HLA, nonidentical, T-cell-depleted bone marrow transplantation. (medscape.com)
  • These new insights, gained from analyzing single HSC grafts, can be used to improve bone marrow transplantation for patients. (usc.edu)
  • In many cases, successfully mobilization is not effected in the patient and inadequate numbers of hematopoietic stem cells are harvested from these patients using current methods. (justia.com)
  • 25 Severe combined immunodefieciency patients: immune recovery after stem cell transplantation. (vinastemcelllab.com)
  • Eight patients (16 %) did not respond to treatment, and one died shortly after transplantation. (springermedizin.at)
  • Together, both IST and stem cell transplantation are reasonable treatment options for patients with AA. (springermedizin.at)
  • The results seen with the recently approved novel agent blinatumomab ( Blincyto , Amgen Inc) are "notable," say authors reporting a trial in which monotherapy produced complete remission in almost half of patients with relapses or refractory B-precursor acute lymphoblastic leukemia (ALL). (medscape.com)
  • A third of these patients subsequently underwent allogeneic hemopoietic stem cell transplantation. (medscape.com)
  • The drug was recently approved by the US Food and Drug Administration for patients with Philadelphia chromosome-negative precursor B-cell ALL who have relapsed or who were refractory to previous treatment. (medscape.com)
  • The study reported by Dr Topp and colleagues enrolled 189 patients with Philadelphia chromosome-negative, primary refractory or relapsed disease (first relapse within 12 months of first remission, relapse within 12 months after allogeneic hemopoietic stem cell transplantation, or no response to or relapse after first salvage therapy or beyond). (medscape.com)
  • Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
  • En outre, il y avait une aug- mentation significative de la forme soluble du c-kit dans le sérum des patients atteints de pemphigus vulgaire actif par rapport aux témoins. (who.int)
  • MDS is a bone marrow disease of unknown etiology that occurs most often in older patients and manifests as progressive cytopenias that occur over months to years. (medscape.com)
  • In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
  • Telomerase can add telomeric repeats onto the chromosome ends, and prevents the replication-dependent loss of telomere and cellular senescence in highly proliferative cells of the germline and in the majority of cancers ( Blasco, 2005 ). (nature.com)
  • Blinatumomab engages the T lymphcytes to destroy leukemia cells (FDA, 2014). (southcarolinablues.com)
  • This information will be integrated with various biochemical approaches including metabolic inhibition of glycosylation and cell surface glycoengineering to custom-modify sLeX display to assess the extent to which sLeX presentation on a specific protein and/or lipid scaffold licenses E-selectin binding among blasts from various subtypes of human acute leukemias, and the impact of the relevant E-selectin receptor/ligand interaction(s) in leukemia cell biology. (cancer.gov)
  • The results of proposed studies will be key to elucidating the glycobiology of leukemogenesis, and should also pro- vide fundamental insights for establishing novel strategies to treat acute leukemias by selectively interrupting sLeX expression and/or E-selectin ligand-mediated processes, and for potential therapy/prognosis stratification schemas based on sLeX levels and/or the expression of distinct E-selectin ligands on acute leukemia cells. (cancer.gov)
  • Relevance: Acute leukemia arises because abnormal white cell precursors proliferate within special growth zones within the bone marrow. (cancer.gov)
  • Although these disorders share certain clinical features-including bone marrow hypercellularity, frequent splenomegaly, and risks of thrombosis, hemorrhage, and transformation to acute myeloid leukemia (AML)-they also show important phenotypic differences. (oncohemakey.com)
  • The image below shows pre-B-cell lymphoblastic leukemia/lymphoma (B-ALL). (medscape.com)
  • Acute myelogenous leukemia (AML) is a malignant disease of the bone marrow in which hematopoietic precursors are arrested in an early stage of development. (medscape.com)
  • RUNX1-positive leukemia cells in preclinical models. (lu.se)
  • Indeed, the potent pathotropic migratory properties of BMSCs and ability to circumvent both the complications associated with immune rejection of allogenic cells and many of the moral reasons associated with embryonic stem cell use suggest that BMSCs are most promising stem cells as a potential target for the clinical use of genetically engineered stem cells [ 14 , 15 ]. (hindawi.com)
  • This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
  • This will be coupled with next generation sequencing based approaches to understanding the consequences of perturbing immune cell function. (lu.se)
  • The precise mechanism remains unclear, but in the majority of acquired cases, the mechanism involves an immune attack on the hematopoietic stem cell. (merckmanuals.com)
  • Although successful in utero hematopoietic cell transplantation (IUHCT) of X-linked severe combined immune deficiency (X-SCID) with enriched stem and progenitor cells was achieved more than a decade ago, it remains applied only in rare cases. (ox.ac.uk)
  • The proposed tolerance of the fetal immune system to foreign human leukocyte antigen early in gestation, a main rationale behind IUHCT, has recently been challenged by evidence for a considerable immune barrier against in utero transplanted allogeneic bone marrow cells. (ox.ac.uk)
  • Based on the pathophysiology of diabetes mellitus, stem cell therapy targets two mechanisms, namely cell replacement and immune correction. (vinastemcelllab.com)
  • The aim of this series of studies was to evaluate the efficiency of diabetic treatment by combining cell replacement therapy with immune correction therapy. (vinastemcelllab.com)
  • This strategy, along with others, is harnessing our immune system to target cancer cells. (medscape.com)
  • However, blinatumomab's dependence on circulating immune cells may limit the ability to combine it with myelosuppressive therapies, Dr Thomas notes. (medscape.com)
  • Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
  • However, obtaining primary neuronal cells from adult tissue is difficult and faces major ethical issues in clinical practice. (hindawi.com)
  • With improved understanding of tissue healing and regeneration, stem cell Prolotherapy is gaining significant clinical importance and potential. (journalofprolotherapy.com)
  • Starting with cell source, choice of donor and recipient, as well as isolation methods, we will then discuss existing expansion protocols (two-/three-dimensional cultivation, basal medium, medium supplements, static/dynamic conditions, and hypoxic/normoxic conditions) and influence of these strategies on the cell functionality after implantation. (frontiersin.org)
  • The malignant cells of ALL are lymphoid precursor cells (ie, lymphoblasts) that are arrested in an early stage of development. (medscape.com)
  • In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
  • In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
  • 21 The cancer stem cell hypothesis and its impact on the design of new cancer therapies. (vinastemcelllab.com)
  • Rejuvenation therapies aim to reverse or repair age-related cellular changes such as molecular waste, calcification , tissue stiffening , loss of stem cell function , genetic alterations, and impaired energy production . (fightaging.org)
  • However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
  • Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)
  • One explanation for deficient repair is when undifferentiated adult stem repair cells are inadequate in number or cannot be stimulated within the damaged tissue site. (journalofprolotherapy.com)
  • Here, we summarise the mechanisms contributing to adipose tissue (AT) plasticity and function including characteristics and cellular complexity of the various adipose depots and we discuss recent insights into AT origins, identification of adipose precursors, pathophysiological regulation of adipogenesis and its relation to WAT/BAT expandability in obesity and its associated comorbidities. (springer.com)
  • Progenitor cells play a crucial role in tissue repair and regeneration. (thenoveldifference.com)
  • When specific tissue or organ damage occurs, these cells can rapidly generate new cells of the desired type to facilitate the healing process. (thenoveldifference.com)
  • They are committed to a specific cell lineage but retain the ability to generate a limited range of related cell types, making them essential for tissue repair, regeneration, and potential medical interventions. (thenoveldifference.com)
  • 10 The potential of stem cells and tissue engineered scaffolds for repair of the central nervous system. (vinastemcelllab.com)
  • Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
  • Quantitating the CD34+ cell population can also be useful during mobilization as well as for determining the optimal timing of apheresis sessions to make sure that enough CD34+ cells have been harvested. (bdbiosciences.com)
  • We have recently demonstrated in a Phase I study in healthy volunteers that single dose of the CXCR4 antagonist BL-8040 induces a rapid and robust mobilization and mega dose collection of human CD34+ stem and progenitor cells. (biokine.com)
  • Thus homeostasis of T regulatory cells is an overbearing requirement for prevention of autoimmune disorders arising from uncontrolled self-tolerance which also permits malignancies to occur. (hoajonline.com)
  • Pemphigus vulgaris (PV) is a life-threate- disorders and to shed a light on the role of ning blistering skin disease in which pa- mast cells in autoimmune diseases [ 7 ]. (who.int)
  • In a recent study, we used droplet-based, single-cell ATAC-seq technology (scATAC-seq) to reveal the epigenetic profile of the transit-amplifying subset of thymic epithelial cells (TECs), which was identified previously using single-cell RNA-sequencing technology (scRNA-seq). (bvsalud.org)
  • In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
  • FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling. (cancerindex.org)
  • This reduced differentiation of CD34+ hematopoietic progenitor stem cells could occur due to both infectious and non-communicable diseases and also due to their drug induced treatments. (hoajonline.com)
  • These cells are committed to developing into a specific type of cell but still retain the capacity to proliferate and generate a limited range of related cell types. (thenoveldifference.com)
  • Our data provide evidence that adult stem cells in bone marrow can be efficiently transduced vector administration without preconditioning. (eyesoftheelephants.com)
  • Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)
  • Myeloma cells destroy bone , which releases calcium, a condition called hypercalcemia. (webmd.com)
  • Abnormal proteins from myeloma cells can also damage the kidneys. (webmd.com)
  • An accurate measurement of CD34 is critical for dose requirement protocols in stem cell transplantation. (bdbiosciences.com)
  • Single-platform flow cytometric absolute cell counting protocols have been shown to provide increased robustness of CD34 enumeration by limiting potential sources of imprecision. (bdbiosciences.com)
  • Apheresis for collection of CD34 cells will be performed on day 5. (biokine.com)
  • An additional 3 apheresis sessions may be conducted if needed in order to reach the goal of ≥ 6×106 mobilized CD34 cells/kg. (biokine.com)
  • The best hope for regeneration of these permanently damaged cells is optic stem cell therapy combined with continued drug treatments or other exogenous biomaterial implants depending upon the relevant ophthalmological condition. (hoajonline.com)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • E-selectin is typically not displayed on resting vascular endothelial cells, and its expression is induced by inflammatory cytokines such as TNF and IL-1. (cancer.gov)
  • Increased expression of inflammatory cytokines, lysyl oxidase, transforming growth factor-β, impaired megakaryocyte function, and aberrant JAK-STAT signaling have all been implicated in the pathogenesis of bone marrow fibrosis. (haematologica.org)
  • CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)
  • Leukocytic, hematopoietic cells are important in maintaining the body's defense against disease. (justia.com)
  • This process of division and differentiation is subject to regulation at many levels to control cell production. (justia.com)
  • Progenitor cells, also known as precursor cells, are a type of cell that falls somewhere between stem cells and fully differentiated, specialized cells in terms of their differentiation potential. (thenoveldifference.com)
  • 5 Differentiation of periodontal stem/progenitor cells: roles of tgf-β1. (vinastemcelllab.com)
  • 9 Tendon injury: role of differentiation of aduilt and embryonic derived stem cells. (vinastemcelllab.com)