Hematopoietic stem cell gene. Medical search. Frequent questions

Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing the bacterial gene. (nature.com)
By contrast, transplantation of wild-type bone marrow stem cells restored memory capacities and diminished soluble Aβ accumulation in APP(Swe)/PS1 and APP(Swe)/PS1/CCR2⁻/⁻ mice. (nih.gov)
Recent clinical successes for gene therapies involving autologous HSC transplantation (HSCT) demonstrate the potential of genetic engineering in this stem cell type for curing disease. (ox.ac.uk)
Cytomegalovirus reactivation is still a critical concern following allogeneic hematopoietic cell transplantation, and cellular immune reconstitution of cytomegalovirus-specific cytotoxic T-cells is necessary for the long-term control of cytomegalovirus reactivation after allogeneic hematopoietic cell transplantation. (nature.com)
These methods and results can help us to better understand immune reconstitution following hematopoietic cell transplantation, leading to future studies on the clinical application of adoptive T-cell therapies. (nature.com)
As genetic aberrations are the main causes of all forms of hereditary anemia, the optimal approach involves repairing the defective gene, possibly through the transplantation of normal hematopoietic stem cells (HSCs) from a normal matching donor or through gene therapy approaches (either in vivo or ex vivo) to correct the patient's HSCs. (sscb-stembiotech.com)
In conclusion, gene-corrected hematopoietic stem cell transplantation has promising outcomes for SCD, Fanconi anemia, and thalassemia, and it may overcome the limitation of the source of allogenic bone marrow transplantation. (sscb-stembiotech.com)
Pediatric patients undergoing allogeneic hematologic stem cell transplantation (HSCT) may experience gut-localized or extraintestinal microsporidiosis by Encephalitozoon spp ( 3 ). (cdc.gov)
How to Facilitate Decision-Making for Hematopoietic Stem Cell Transplantation in Patients With Hemoglobinopathies. (thalassaemia.org.cy)
Allogeneic hematopoietic transplantation may be curative in some patients with thalassemia major. (medscape.com)
2020 was an important year for Gamida Cell as we made significant progress across our entire pipeline, including omidubicel, which is poised to become the first FDA approved cell therapy for bone marrow transplantation. (seekingalpha.com)
The primary endpoint of the study was time to neutrophil engraftment, or the time it took for the white blood cells to recover following transplantation. (seekingalpha.com)
Advancing Transplantation, Gene and Cell Therapy - An inaugural symposium honoring Dr. E. Donnall Thomas and his legacy of transplantation. (fredhutch.org)
This first-ever symposium featured leading researchers from around the world sharing their current research on improving survival after hematopoietic transplantation, adoptive cell therapy, gene therapy and hybrid therapies. (fredhutch.org)
This book includes discussions of management and treatment strategies for hemoglobin disorders, such as transfusions, iron-chelating agents, gene therapy, and stem cell transplantation. (cshlpress.com)
By 6 months post-transplantation, the reconstituted mice had developed a clonal myeloproliferative/myelodysplastic disorder originating from the cells with aberrantly reduced Mybl2 expression. (elifesciences.org)
Hematopoietic stem cell transplantation (bone marrow, cord blood, or peripheral blood stem cells) may cure aplastic anemia and prevent myelodysplastic syndrome or leukemia. (medscape.com)
Hematopoietic stem cell transplantation is currently an inpatient procedure. (medscape.com)
A study by MacMillan et al reported that alternative donor hematopoietic cell transplantation had a high success rate in patients with Fanconi anemia who did not have a history of opportunistic infections or transfusions and who underwent conditioning with single fraction total body irradiation 300 cGy, cyclophosphamide, fludarabine, and antithymocyte globulin. (medscape.com)
Although the only therapy that can cure the pancytopenia is stem cell transplantation, androgens, to which approximately 50-75% of patients respond, are used for those in whom transplantation is not an option. (medscape.com)
Hematopoietic stem cells (HSCs) enable hematopoietic stem cell transplantation (HCT) through their ability to replenish the entire blood system. (lu.se)
We isolated bone marrow from mice at 8 time points after transplantation and examined the reconstitution dynamics and transcriptional profiles of stem and progenitor populations. (lu.se)
We used a decay rate model to fit the temporal transcription profiles of regenerating HSCs and identified genes with progressively decreased or increased expression after transplantation. (lu.se)
Understanding how HSPCs are regulated is of central importance for several areas in clinical hematology, including stem cell transplantation and the treatment of leukemias. (lu.se)
Allogeneic hematopoietic stem cell transplantation for X-linked ectodermal dysplasia and immunodeficiency: case report and review of outcomes. (lu.se)
Participants aged =18 with Newly Diagnosed Multiple Myeloma (NDMM) who has received induction therapy followed by high-dose chemotherapy and autologous stem cell transplantation (ASCT), without subsequent consolidation or maintenance. (who.int)
Participant has previous history of an allogeneic hematopoietic stem cell transplantation or treatment with any gene therapy-based therapeutic for cancer or investigational cellular therapy for cancer or B-cell maturation antigen targeted therapy. (who.int)

This event requires a special environment, termed the hematopoietic stem cell niche, which provides the protection and signals necessary to carry out the differentiation of cells from HSC progenitors. (wikipedia.org)
Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
Additionally, edited CD34 + cells can be further cultured in conditions stimulating lineage-specific differentiation by combining StemSpan™ media with expansion supplements designed for the expansion and differentiation of progenitors to erythroid, myeloid, megakaryocyte, natural killer (NK), or T cell lineages. (stemcell.com)
Research in the Paralkar Lab spans the spectrum from human patient sample studies and mouse models to cutting-edge molecular biology tools, high-throughput sequencing approaches, and novel computational algorithms, all with the goal of gaining insight into how the transcription of coding genes and noncoding ribosomal DNA genes is regulated in hematopoietic stem cells, myeloid progenitors, and in leukemia. (upenn.edu)
In a murine competitive reconstitution model, Mybl2 knockdown by RNAi to 20-30% of normal levels in multipotent hematopoietic progenitors resulted in clonal dominance of these 'sub-haploinsufficient' cells, which was reflected in all blood cell lineages. (elifesciences.org)
We conclude that downregulation of MYBL2 activity below levels predicted by classical haploinsufficiency underlies the clonal expansion of hematopoietic progenitors in a large fraction of human myeloid malignancies. (elifesciences.org)
One focus of our lab is differentiating mouse ESC into intestinal epithelial cells in order to understand the complex signaling pathways involved in intestinal commitment from endodermal progenitors and undifferentiated stem cells. (bu.edu)
We are combining novel engineered stromal cells with gene editing to explore a way to direct the differentiation of iPSC into hematopoietic progenitors and into a T cell/T reg lineage. (bu.edu)

Dexter observed that mesenchymal stromal cells could maintain early HSCs ex vivo, and both Lord and Gong showed that these cells localized to the endosteal margins in long bones. (wikipedia.org)
Cell and gene therapies using haematopoietic stem cells (HSCs) epitomize the transformative potential of regenerative medicine. (ox.ac.uk)
With recent advances in CRISPR gene-editing technologies, methodologies for the ex vivo expansion of HSCs and non-genotoxic conditioning protocols, the range of clinical indications for HSC-based gene therapies is expected to significantly expand. (ox.ac.uk)
These include pre-existing immunity to gene-therapy reagents, immune responses to neoantigens introduced into HSCs by genetic engineering, and unique challenges associated with next-generation and off-the-shelf HSC products. (ox.ac.uk)
Moreover, we expound the future research direction of HSC derivation from induced pluripotent stem cells (iPSCs), strategies to edit HSCs, gene therapy risk mitigation, and their clinical perspectives. (sscb-stembiotech.com)
Our studies have revealed that correct pattern of HOXA gene activity is essential for specifying human HSCs and maintaining their function. (ca.gov)
The major shortcoming in generating functional hematopoietic stem cells (HSC) from pluripotent stem cells is the lack of adequate markers for specification and maintenance of functional human HSCs. (ca.gov)
The group are currently focused on the pivotal role that HOXA cluster genes play in the demarcation and possible emergence of haematopoietic stem cells (HSCs) and their role in cancer. (nottingham.ac.uk)
However, eradication of the leukaemia repopulating cell or stem cell whilst retaining normal HSCs remains a challenge with current experimental therapies. (nottingham.ac.uk)
Hematopoietic Stem Cells (HSCs) are the most thoroughly characterized stem cell population in the body and their study has resulted in well established methods for their isolation, purification and reliable assays of HSC function. (bu.edu)
During the last few years we have substantially improved our ability to genetically manipulate HSCs using viral vectors for gene transfer. (bu.edu)
Moreover, we observed molecular changes in cell cycle, MYC and mTOR signaling in both HSCs, and progenitor subsets. (lu.se)
Taken together, our study provides a detailed functional and molecular characterization of HSCs at different phases of regeneration and identifies a gene set associated with the transition from proliferation to quiescence. (lu.se)
It is believed that benzene and its metabolites target hematopoietic stem cells (HSCs) to cause toxicity and cancer in the hematopoietic system. (cdc.gov)
In the current study, we compared the effects of hydroquinone (HQ), a major metabolite of benzene in humans and animals, on mouse embryonic yolk sac hematopoietic stem cells (YS-HSCs) and adult bone marrow hematopoietic stem cells (BM-HSCs). (cdc.gov)
Differences in gene expression profiles were observed in HQ-treated YS-HSCs and BM-HSCs. (cdc.gov)
Culture conditions in which hematopoietic stem cells (HSCs) can be expanded for clinical benefit are highly sought after. (lu.se)
To elucidate regulatory mechanisms governing the maintenance and propagation of human HSCs ex vivo, we screened libraries of annotated small molecules in human cord blood (CB) cells using an optimized assay for detection of functional HSCs during culture. (lu.se)
Purified HSCs treated with CPX showed a reduced cell division rate and an enrichment of HSC- specific gene expression patterns. (lu.se)

Many human blood cells, such as red blood cells (RBCs), immune cells, and even platelets all originate from the same progenitor cell, the hematopoietic stem cell (HSC). (wikipedia.org)
Hematopoiesis involves a series of differentiation steps from one progenitor cell to a more committed cell type, forming the recognizable tree seen in the adjacent diagram. (wikipedia.org)
The CMP can then further differentiate into the megakaryocyte-erythroid progenitor cell (MEP), which goes on to make RBCs and platelets, or the granulocyte/macrophage progenitor (GMP), which gives rise to the granulocytes of the innate immune response. (wikipedia.org)
The hemangioblast theory, which posits that the RBCs and ECs derive from a common progenitor cell, was developed as researchers observed that receptor knockout mice, such as Flk1-/-, exhibited defective RBC formation and vessel growth. (wikipedia.org)
Progenitor cell function may be measured post-modification by in vitro CFU assays. (stemcell.com)
A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish. (bvsalud.org)

Develop lentiviral vectors carrying human alpha-globin gene for gene therapy of alpha thalassemia (AT) and perform initial tests in a cell line. (ca.gov)
Patients with Wiskott-Aldrich syndrome (WAS) lacking a human leukocyte antigen-matched donor may benefit from gene therapy through the provision of gene-corrected, autologous hematopoietic stem/progenitor cells. (ucl.ac.uk)
NCT02333760 ) for eight patients with WAS having undergone phase I/II lentiviral vector-based gene therapy trials (nos. (ucl.ac.uk)
Primary outcomes of the long-term study were to establish clinical and biological safety, efficacy and tolerability by evaluating the incidence and type of serious adverse events and clinical status and biological parameters including lentiviral genomic integration sites in different cell subpopulations from 3 years to 15 years after gene therapy. (ucl.ac.uk)
The results suggest that lentiviral gene therapy provides sustained clinical benefits for patients with WAS. (ucl.ac.uk)
Finally, gene therapy using a lentivirus-expressing CCR2 transgene in BMCs prevented cognitive decline in this mouse model of AD. (nih.gov)
To clearly illustrate the importance of cell and gene therapy in hereditary anemia, this paper provides a review of the genetic aberration, epidemiology, clinical features, current management, and cell and gene therapy endeavors related to SCD, thalassemia, Fanconi anemia, and G6PDD. (sscb-stembiotech.com)
The small compounds boost transduction into a variety of cells including other types of stem cells and T cells for CAR T therapy. (seattlechildrens.org)
In addition to improving gene therapy and delivery of gene-editing tools into cells, increasing transduction efficiency using small molecule agents could facilitate using of hemopoietic stem cells for protective functions. (seattlechildrens.org)
The Torbett lab is developing these methods for multiple types of stem cells and for applications in CAR T cell therapy. (seattlechildrens.org)
Since the first successful gene therapy for thalassemia major, in 2007, researchers have worked to improve the efficacy and safety of the procedure. (medscape.com)
And GDA-201, an innate natural killer cell or NK cell therapy. (seekingalpha.com)
Most of these clinicians had never cared for a child with SMA before, nor did they know that lifesaving gene therapy for the condition now exists. (medscape.com)
The newest addition to the list of approvals is Elevidys, Sarepta's gene therapy for Duchenne muscular dystrophy (DMD). (medscape.com)
Within the decade, a significant fraction of the pediatric population will have gone through gene therapy, experts told Medscape Medical News . (medscape.com)
These cases - some before gene therapy and some after - will show up in primary care offices before the textbook is written. (medscape.com)
Even now, gene therapy is sequestered away in large academic medical research centers. (medscape.com)
Although they probably will not manage gene-therapy patients on their own - co-managing them instead with subspecialists - they will be involved in the ordering and monitoring of safety labs and other tests. (medscape.com)
Some of the side effects that come with gene therapy are established. (medscape.com)
In the 30-90 days after gene therapy, patients need a lot of follow-up for safety reasons. (medscape.com)
But aftercare for gene therapy will be more than just monitoring and managing side effects. (medscape.com)
In some cases, gene therapy may largely eliminate the disease. (medscape.com)

In this study, we observed that the expression of pseudouridine (Ψ) synthase 10 is increased in aged hematopoietic stem and progenitor cells (HSPC) and enforced protein of Ψ synthase 10 (PUS10) recapitulates the phenotype of aged HSC, which is not achieved by its Ψ synthase activity. (haematologica.org)
Hematopoietic stem/progenitor cells (HSPC) are known to be functionally diverse in terms of their self-renewal potential and lineage output. (lu.se)
Consequently, investigation of molecular heterogeneity in the differentiation potential of HSPC is vital to identify novel regulators that affect generation of specific cell types, especially RBC. (lu.se)
Importantly, we identified an endothelial cell -specific G protein -coupled receptor 182, followed by in vivo and in vitro functional validation of its evolutionally conserved role in supporting HSPC expansion in zebrafish and mice . (bvsalud.org)
These findings enhance our understanding of the regulatory mechanism underlying hematopoietic niche for HSPC expansion in vivo and provide insights into improving protocols for HSPC expansion in vitro . (bvsalud.org)
In a second line, we use natural genetic variation to find genes that regulate hematopoietic stem and progenitor cells (HSPC) in humans. (lu.se)

Molecular changes underlying stem cell differentiation are of fundamental interest. (nih.gov)
This study reveals effects of cell cycle progression on the expression of lineage specific genes in precursor cells, and suggests that hematopoietic stress changes the balance of renewal and differentiation in these homeostatic cells. (nih.gov)
In early blood cells, called hematopoietic stem cells, the methylation patterns established by DNA methyltransferase 3 alpha promote maturation (differentiation) into different blood cell types. (medlineplus.gov)
As in cytogenetically normal acute myeloid leukemia (described above), the mutations disrupt the normal pattern of methylation in cells, which blocks differentiation. (medlineplus.gov)
Our studies also suggest that these hESC reporter lines will become valuable for investigators working on optimizing stem cell differentiation and culture for other tissues, as many of these reporter genes are not only restricted to HSC. (ca.gov)
These lines will serve as tools to identify factor combinations to improve the in vitro differentiation of hESCs to functional blood stem cells. (ca.gov)
Melinda is highly proficient in the use of in vitro and in vivo assays for normal and malignant hematopoietic stem and progenitor cells, determination of self-renewal, expansion and lineage specific differentiation capabilities, and the use of induced pluripotent stem cells for the investigation of hematopoietic disorders and leukemic progression and the testing of targeted therapeutics. (edu.au)
Aged hematopoietic stem cells (HSC) exhibit compromised reconstitution capacity and differentiation-bias towards myeloid lineage, however, the molecular mechanism behind it remains not fully understood. (haematologica.org)
erythroid cells after in vitro differentiation, despite having similar frequencies of phenotypic HSC initially. (lu.se)
Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. (nih.gov)
Dr. Robertson, who also works in breast cancer and brain tumors as well as colon cancer, is finding patterns of genes that regulate healthy cell differentiation are shut off in cancers. (sciencedaily.com)
One major focus of the lab is the utilization of iPS Cells for the study of intestinal differentiation with a particular interest in Colorectal Carcinoma (CRC). (bu.edu)
By studying the earlier events associated with intestinal differentiation comparing the normal and mutant cells we aim at discovering the basic mechanisms involved in tumor development in the gastrointestinal tract. (bu.edu)
Despite these efforts, few genes are known to play a role in the processes of stem cell self-renewal and differentiation. (bu.edu)
[ 18 ] Arylsulfatase A deficiency leads to defective glial and neuronal differentiation from neural progenitor cells. (medscape.com)
During vertebrate embryogenesis , fetal hematopoietic stem and progenitor cells (HSPCs) exhibit expansion and differentiation properties in a supportive hematopoietic niche. (bvsalud.org)

[ 14 ] The first successful allogeneic stem cell transplant from an HLA-identical sibling donor was reported in 1982. (medscape.com)

Lentiviral gene transfer and expression in c-kit+sca1+mouse hematopoietic stem cells. (ox.ac.uk)

Our lab has a major interest in the study of induced Pluripotent Stem cells or iPS cells and the development of tools for their generation and characterization. (bu.edu)
Pioneering work by the laboratory of Dr. Yamanaka showed that fibroblasts transduced with retroviral vectors expressing four transcription factors, Oct4, Klf4, Sox2 and cMyc can be reprogrammed to become pluripotent stem cells that appear almost indistinguishable from ESC. (bu.edu)

Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
Cells previously known as endothelial progenitor cells are considered now to be rather myeloid angiogenic cells than endothelial precursors [ 4 ]. (hindawi.com)
Therefore, the cells we used should be considered as hematopoietic precursors of myeloid angiogenic cells and are referred as PACs. (hindawi.com)
Mutations in the DNMT3A gene are associated with a form of blood cancer known as cytogenetically normal acute myeloid leukemia (CN-AML). (medlineplus.gov)
Researchers speculate that the altered gene activity prevents hematopoietic stem cells from differentiating normally, which leads to the overproduction of abnormal, immature white blood cells characteristic of acute myeloid leukemia. (medlineplus.gov)
It is unclear why some people with DNMT3A gene mutations develop acute myeloid leukemia and others develop acute lymphoblastic leukemia. (medlineplus.gov)
Inhibition of HOX/PBX dimer formation leads to necroptosis in acute myeloid leukemia cells ONCOTARGET. (nottingham.ac.uk)
Abnormalities in this process lead to a group of diseases known as myeloid malignancies, which include acute myeloid leukaemia-in which the bone marrow produces abnormal white blood cells-and myelodysplastic syndromes, which are caused by too few mature blood cells being produced. (elifesciences.org)
This deletion is only ever seen in one of their two copies of the chromosome-suggesting that at least some of these genes are essential for survival-but the identity of the gene(s) that are associated with the increased risk of myeloid malignancies is unknown. (elifesciences.org)
Myeloid malignancy patients lacking one copy of this gene showed levels of MYBL2 expression that were less than 50% of those in healthy individuals. (elifesciences.org)
used gene silencing techniques to reduce the expression of MYBL2 in mice and showed that this induced symptoms of myeloid malignancies in the animals. (elifesciences.org)
In addition to revealing a new tumor suppressor gene and its contribution to myeloid malignancies, the study by Heinrichs et al. (elifesciences.org)
Our long standing interest in the immune system now focuses in the utilization of iPSC-derived immune cells, including myeloid dendritic cells (mDCs), T cells and others as the basis to study several inflammatory diseases such as lupus erythematosus and the signaling cascade responsible for immune dysregulation. (bu.edu)
We develop a computational model for the hematopoietic erythroid/myeloid lineage decision, which is determined by a genetic switch involving the genes PU.1 and GATA-1. (lu.se)

This approach affords an efficient and rapid means of re-introducing genetically modified tissue into intact organisms and provides a system whereby the expression and regulation of cloned genes can be followed within the context of a well characterized developmental programme. (nature.com)
Decreased DNA methylation likely disrupts the normal regulation of important developmental genes, although how these change cause the specific features of DNMT3A overgrowth syndrome is unknown. (medlineplus.gov)
The Paralkar Lab has identified that key hematopoietic and leukemic transcription factors bind to rDNA and regulate rRNA transcription, and we are interested in understanding how the binding of cell-type-specific transcription factors regulates the activity of Polymerase I and the transcription of rRNA in normal hematopoiesis, and how this regulation is co-opted in leukemia to drive abundant ribosome biogenesis. (upenn.edu)
In early 2008, the National Institutes of Health established an epigenomics program to coordinate such efforts to better understand how this method of gene regulation fits into normal development, aging, learning and memory as well as its role in cancer, obesity, depression and other disease. (sciencedaily.com)
RNA splicing is a major nexus of gene expression regulation, shaping cellular identity during development, frequently altered in human cancers. (lu.se)

Test the activities of the vectors in hematopoietic stem cells from healthy donor in culture and by growing in immune deficient mice. (ca.gov)
We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)
However, little information is available on the gene expression profile (GEP) of CMV-CTL clones according to CMV reactivation patterns (and donor CMV serostatus) after allo-HCT. (nature.com)
Dr. E. Donnall Thomas and his colleagues discovered a way to treat advanced leukemia by eradicating malignant white blood cells in the bone marrow using high doses of chemotherapy and radiation, and then replacing them with healthy donor cells. (fredhutch.org)

At least 16 mutations in the DNMT3A gene have been found to cause DNMT3A overgrowth syndrome. (medlineplus.gov)
DNMT3A gene mutations that cause DNMT3A overgrowth syndrome are found in all of the body's cells and lead to a decrease in normal enzyme function. (medlineplus.gov)
Some of these DNMT3A gene mutations lead to a decrease in normal enzyme function. (medlineplus.gov)
they are typically found in a small percentage of cells and the mutations are not inherited. (medlineplus.gov)
Somatic DNMT3A gene mutations are also found relatively frequently in another form of blood cancer called T-cell acute lymphoblastic leukemia. (medlineplus.gov)
Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. (nih.gov)
We are investigating the impact of specific classes of gene mutations, such as ATM, BRCA1, and MYBL2 on genome integrity. (birmingham.ac.uk)
We aim at generating iPSC containing mutations in IRF5, Myd88, UNC93, TLRs and other relevant genes to test their role in mDC function compared to normal cells. (bu.edu)
These defects result from different gene mutations, mostly in the ARSA gene, and many new causative mutations have been identified. (medscape.com)
Wiskott-Aldrich syndrome is caused by mutations in the gene that encodes the Wiskott-Aldrich syndrome protein (WASP), a cytoplasmic protein necessary for normal B- and T-cell signaling. (msdmanuals.com)
Mutations on epigenetic regulator genes are common in clonal hematopoiesis and may be a risk factor for HEMATOLOGIC NEOPLASMS and other cardiovascular diseases. (bvsalud.org)
Splicing factor mutations are particularly prevalent in MDS, a group of heterogeneous hematological disorders characterized by defective blood stem cells and a high risk of leukemia development. (lu.se)
NEMO gene mutations in Chinese patients with incontinentia pigmenti. (lu.se)

In 1978, after observing that the prototypical colony-forming stem cells were less capable at replacing differentiated cells than bone marrow cells injected into irradiated animals, Schofield proposed that a specialized environment in the bone marrow allows these precursor cells to maintain their cellular reconstitution potential. (wikipedia.org)
These studies and others supported the idea that bone cells create the HSC niche, and all the research that elucidated this specialized hematopoietic microenvironment stemmed from these landmark studies. (wikipedia.org)
An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. (nature.com)
Here we show that these mechanisms specifically require CC-chemokine receptor 2 (CCR2) expression in bone marrow cells (BMCs). (nih.gov)
The hematopoietic stem cells in the bone marrow continuously ensure that the blood system is supplied with new cells throughout life and that in stressful conditions, such as infections, inflammations or bleeding, the production of the required blood cells can then be initiated immediately. (idw-online.de)
Normal hematopoiesis requires an intricate balance in the bone marrow between the ability of stem cells to maintain themselves for decades of life while producing billions of mature blood cells every day. (upenn.edu)
Acute lymphocytic leukemia (ALL) in children is a clonal disease of bone marrow hematopoietic stem cells. (medscimonit.com)
Blood cells are produced within bone marrow by specialized stem cells and progenitor cells. (elifesciences.org)
Comparison of toxicity of benzene metabolite hydroquinone in hematopoietic stem cells derived from murine embryonic yolk sac and adult bone marrow. (cdc.gov)

scRNA-seq on murine hematopoietic stem cells (HSC) and their progeny MPP1 separated the cells into 3 main clusters with distinct features: active, quiescent, and an un-characterized cluster. (nih.gov)

These include determining whether the instructions in a particular segment of DNA are carried out or suppressed (gene silencing), regulating reactions involving proteins and fats, and controlling the processing of chemicals that relay signals in the nervous system (neurotransmitters). (medlineplus.gov)
In this scenario, vectors would deliver genes for immunoadhesives - proteins that B cells would produce from introduced genes-to bind and neutralize specific pathogens. (seattlechildrens.org)
This balance is maintained by the combinatorial activity of transcription factors and chromatin proteins that dictate the transcription of coding gene networks instructing fate choice decisions. (upenn.edu)
Solid as well as blood cancers are associated with overexpression of HOX proteins in the nucleus, which is traditionally a difficult area of the cell to target with conventional drugs. (nottingham.ac.uk)
Cells gain the ability to make lifesaving proteins - proteins that heal wounds , restore muscle function , and fight cancer . (medscape.com)
This classification was abandoned because of the observation that surface proteins were missing not only in the RBC membrane but also in all blood cells, including the platelet and white cells. (medscape.com)
The common denominator in the disease, a biochemical defect, appears to be a genetic mutation leading to the inability to synthesize the glycosyl-phosphatidylinositol (GPI) anchor that binds these proteins to cell membranes. (medscape.com)
Due to its location on the X chromosome, and due to X inactivation in female somatic cells, only one mutation is required in either males or females to abolish the expression of GPI-linked proteins. (medscape.com)
This process is mediated by a complex molecular machinery known as the spliceosome, which enables the production of multiple and functionally distinct proteins from single genes. (lu.se)

Lineage-specific genes were differently expressed between cells, and correlated with the cell cycle stages with a specific augmentation of erythroid related genes in the G2/M phase. (nih.gov)
RNA sequencing revealed that genes involved in G protein-coupled receptor (GPCR) signaling were significantly up-regulated in the high-erythroid output donors. (lu.se)

Genetically engineered hematopoietic stem cells are a promising therapeutic strategy for treating a variety of conditions including HIV infection, cancer, and inherited disorders such as hemophilia and thalassemia's. (seattlechildrens.org)
The ability to genetically manipulate hematopoietic stem and progenitor cells (HSPCs) in particular has significantly advanced our understanding of the mechanisms that regulate hematopoiesis and is contributing to the development of novel cellular therapies. (stemcell.com)
In contrast to ESC, iPS cells are genetically identical to the individual from whom they are derived, raising the prospect of utilizing iPS cells for autologous cell based therapies without risk of rejection. (bu.edu)

CD34 + cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48 h. (hindawi.com)
EPCs are enriched in the population coexpressing CD34, CD133, and KDR with a low or absent expression of pan-hematopoietic marker CD45 [ 1 ]. (hindawi.com)
This Technical Bulletin provides instructions for CRISPR-Cas9 genome editing and maintenance of human CD34 + cells, including optimized pre- and post-editing culture conditions and methods to evaluate genome editing efficiency. (stemcell.com)
While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
The ArciTect™ CRISPR-Cas9 RNP is then prepared and delivered into CD34 + cells using electroporation, and cells are plated in StemSpan™ SFEM II supplemented with StemSpan™ CD34 + Expansion Supplement post-electroporation. (stemcell.com)
We found that the antifungal agent ciclopirox ethanolamine (CPX) selectively supported immature CD34+CD90+ cells during culture and enhanced their long-term in vivo repopulation capacity. (lu.se)

By synthesizing these factors in this Review, we hope to encourage more research to address the immunological issues associated with current and next-generation HSC-based gene therapies to help realize the full potential of this field. (ox.ac.uk)
A better and deeper understanding of immune reconstitution following allo-HCT is required for further clinical application of these adoptive T-cell therapies. (nature.com)
These tools will help translate the promise of stem cells to cell based therapies to treat human disease. (ca.gov)
How can our knowledge of the immune system be applied to develop innovative therapies, including the identification of novel antigens for immune therapies and gene repair strategies in monogenic immune diseases? (mdc-berlin.de)
Today, cell-based therapies have become a standard of care for many patients with cancer and other diseases. (fredhutch.org)
Cell based therapies: Academic or commercial? (ehaweb.org)
Small cell lung cancer (SCLC) is an exceptionally lethal malignancy for which more effective therapies are urgently needed. (cancerindex.org)
General practitioners "need to know side effects because they are going to deal with side effects when someone calls them in the middle of the night," said Beales, who also is chief executive officer of Axovia Therapeutics, a biotech company developing gene therapies. (medscape.com)
Adeno-associated virus (AAV) or AAV-mediated gene therapies carry an increased risk for damage to the heart and liver, Nelson said. (medscape.com)
Identified gene candidates will be studied further in the context of determining sensitivity of protein degrader drugs which are currently emerging as alternative therapies for leukemia. (lu.se)

A year later, Choi showed that blast cells derived from embryonic stem (ES) cells displayed common gene expression of both hematopoietic and endothelial precursors. (wikipedia.org)

rRNA transcription rates vary dramatically between different normal cell types in the hematopoietic tree, and leukemic cells have characteristic prominent nucleoli, indicating robust ribosome synthesis. (upenn.edu)
The thesis work would focus on identification of genes that are essential for the optimal functioning of the ubiquitin-proteasomal system in leukemic cells. (lu.se)

Most type II PNH cells (total lack of GPI-linked protein) are due to a frame shift mutation occurring in the early hematopoietic progenitor cells, resulting in same mutation in all blood cell lines. (medscape.com)

The Paralkar Lab has developed customized genomes and computational pipelines to map datasets to rDNA, and we are interested in developing advanced tools to map and interpret the genetic and epigenetic profiles of rDNA in normal and malignant cells. (upenn.edu)
In a study published in the Molecular Cell journal, they explain what makes hematopoietic stem cells acquire malignant traits in cancer. (lu.se)

All of the MPNs are hematopoietic rates from 2001 (when MPNs first became reportable) stem cell disorders of common clonal heritage, character- through 2005 in these 3 counties. (cdc.gov)
Las mutaciones en genes reguladores epigenéticos son frecuentes en la hematopoyesis clonal y pueden ser un factor de riesgo de NEOPLASIAS HEMATOLÓGICAS y de otras enfermedades cardiovasculares. (bvsalud.org)
Cuando la fracción alélica variante representa al menos el dos por ciento y está presente en ausencia de citopenias intensas, recibe el nombre de hematopoyesis clonal de potencial indeterminado (HCPI). (bvsalud.org)

Examples are CRISPR/Cas9-mediated mutagenesis, genetic mouse models, cell-fate-mapping, organoids and tumor explants, as well as GMP production of pharmaceuticals and many years of experience in conducting clinical studies. (mdc-berlin.de)
The MDC's participating research groups bring a broad range of technological expertise to the initiative - ranging from biochemical assays, structure and function analysis, and gene editing to the development of preclinical mouse models, GMP production, and even clinical trials. (mdc-berlin.de)
The DKFZ is a multidisciplinary center covering cancer biology from basic molecular and cell biology to clinical research and care. (mdc-berlin.de)
Development of pre-clinical models using stem cell technologies. (nottingham.ac.uk)
The clinical impact of glucose-6-phosphate dehydrogenase deficiency in patients with sickle cell disease. (cdc.gov)
The name of the disorder is a descriptive term for the clinical consequence of red blood cell (RBC) breakdown with release of hemoglobin into the urine, which manifests most prominently as dark-colored urine in the morning (see image below). (medscape.com)
Several single gene disorders share clinical and radiologic characteristics with multiple sclerosis and have the potential to be overlooked in the differential diagnostic evaluation of both adult and paediatric patients with multiple sclerosis. (medscape.com)
Here we review single gene disorders that have the potential to mimic multiple sclerosis, provide an overview of clinical and investigational characteristics of each disorder, and present guidelines for when clinicians should suspect an underlying heritable disorder that requires diagnostic confirmation in a patient with a definite or probable diagnosis of multiple sclerosis. (medscape.com)

Up to one-third of people with CN-AML have a mutation in the DNMT3A gene. (medlineplus.gov)
Approximately 2 million African-Americans, or 1 in 12, are "carriers" of the sickle mutation (called sickle cell trait). (childrenshospital.org)
In Colorado and Wyoming, nearly every baby born since 2020 is tested for signs of a mutation in the SMN1 gene, an indicator of spinal muscular atrophy (SMA). (medscape.com)
Paroxysmal nocturnal hemoglobinuria (PNH) was previously classified as purely an acquired hemolytic anemia due to a hematopoietic stem cell mutation defect. (medscape.com)
SUMMARY A direct correlation between HIV infection and mutation in the chemokine receptor ( CCR5 ) gene has been established. (who.int)
HIV-1 is influenced by a mutation in the 1 infection with a typical delay of 2-4 years chemokine receptor ( CCR5 ) gene [ 3,4 ]. (who.int)
Biotech Europe GmbH, Freiburg, Germa- cation of the virus in cells heterozygous for ny) was used to extract genomic DNA the mutation proceeded at an intermediate from white blood cells following the lysis rate [ 5 ]. (who.int)
A novel nonsense mutation of the IKBKG gene. (lu.se)
Incontinentia pigmenti in a newborn with a novel nonsense mutation in the NEMO gene. (lu.se)
Novel splicing mutation in the NEMO (IKK-gamma) gene with severe immunodeficiency and heterogeneity of X-chromosome inactivation. (lu.se)
Transient hemophagocytosis with deficient cellular cytotoxicity, monoclonal immunoglobulin M gammopathy, increased T-cell numbers, and hypomorphic NEMO mutation. (lu.se)
A new mutation in exon 7 of NEMO gene: late skewed X-chromosome inactivation in an incontinentia pigmenti female patient with immunodeficiency. (lu.se)

This gene encodes a receptor tyrosine kinase. (nih.gov)
While each DNA blueprint, or gene, encodes for a different machine or structure, the genome, the collection of DNA wrapped up inside the nucleus, is much more active than a quiet library of neatly stacked blue-prints. (birmingham.ac.uk)
The gene, which is called MYBL2 , encodes a transcription factor that helps to control the cell division cycle. (elifesciences.org)
This gene encodes a member of the trithorax group of transcriptional activators. (cancerindex.org)

The gene Igf2bp2 is important in youth for the full function of these cells, as it activates their growth and metabolism. (idw-online.de)
Researchers at the Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) in Jena have been able to demonstrate that in mice, the growth factor Igf2bp2 controls hematopoietic stem cell function in young adulthood by activating stem cell metabolism and growth. (idw-online.de)
The activity of the metabolism and of growth signals contributes decisively to the development of stem cell function. (idw-online.de)
The experimental findings of the current study suggest that the activation of growth and metabolism in juvenile mice preprograms the subsequent loss of function of hematopoietic stem cells and inscribes this into the cell's memory. (idw-online.de)
Using this expertise, the scientists were able to identify a new subset of hematopoietic stem cells that exhibit particularly strong activity of Igf2bp2-dependent metabolism and growth in adolescent mice. (idw-online.de)

DESCRIPTION (provided by applicant): Therapeutic stem cell gene transfer relies on long-term gene expression achieved by integration of new DNA into the cellular genome. (sbir.gov)
Getting more vectors into cells faster would save patient and clinic time and therapeutic product, resulting in lower-cost, higher-safety treatment. (seattlechildrens.org)
Our goal is to develop tools that address major bottlenecks that have prevented the generation of blood forming stem cells in culture for therapeutic use. (ca.gov)

More than 500 worldwide genotypes of E. bieneusi have been identified based on genetic polymorphisms in the internal transcribed spacer of the rRNA gene. (cdc.gov)
CRISPR-Cas9, an RNA-guided genome editing technology, is revolutionizing cell biology due to the ease and efficiency by which it enables genetic manipulation of mammalian cells. (stemcell.com)
Sickle cell disease (SCD) is an autosomal recessive genetic condition that alters the shape and function of the hemoglobin molecule in red blood cells. (thalassaemia.org.cy)
Prenatal diagnosis involves testing the DNA of fetal or placental cells, obtained through chorionic villous sampling (CVS), amniocentesis, or non-invasive means, to see if the SCID-causing genetic variant is present. (primaryimmune.org)
hemoglobinopathies (e.g., sickle cell disease) and thalassemias are some of the most common human genetic diseases worldwide. (cshlpress.com)
Contributors examine the structure, expression, and evolution of the globin genes, the assembly of globin subunits into functional forms of hemoglobin, and the numerous variants that result from genetic alterations. (cshlpress.com)
It is an indispensable reference for biochemists, geneticists, cell and developmental biologists, physiologists, and all who are interested in reducing the medical burden of these common genetic diseases. (cshlpress.com)
The severity of the symptoms of sickle cell disease can vary greatly depending on the specific genetic type and even within those of the same type. (childrenshospital.org)
Hematopoietic stem cell lineage choices are decided by genetic networks that are turned ON/OFF in a switch-like manner. (lu.se)
A team of researchers led by Dr. Cristian Bellodi recently discovered a hardwired genetic control mechanism modulating individual spliceosomal components, known as splicing factors, in cells harboring oncogenic lesions common in human cancers. (lu.se)
The Nilsson lab focuses on how inborn genetic variation influences blood cell formation and blood cancer risk in humans. (lu.se)
In a first line of research, we study genetic predisposition for multiple myeloma (MM). Studies going back to the 1920s support familial aggregation of MM. Recently, we and others discovered sequence variants influencing MM risk, including common variants at 24 loci and rare, high-penetrant variants in the tumor suppressor gene DIS3. (lu.se)
Recognition of a single-gene disorder as causal for a patient's 'multiple sclerosis-like' phenotype is critically important for accurate direction of patient management, and evokes broader genetic counselling implications for affected families. (medscape.com)

Currently, we lack markers that we can use to pinpoint the developmental stage of human ES cell derived candidate hematopoietic stem cells (HSC), and whether they have established the correct molecular machinery required for proper function. (ca.gov)
To profile the developmental landscape of fetal HSPCs and their local niche, here, using single- cell RNA -sequencing, we deciphered a dynamic atlas covering 28,777 cells and 9 major cell types (23 clusters) of zebrafish caudal hematopoietic tissue (CHT). (bvsalud.org)

By accessing primary human target cells we hope to establish a new paradigm in the use of iPSC-derived cells for infectious diseases. (bu.edu)

We characterized four heterogeneous HSPCs with distinct lineage priming and metabolic gene signatures. (bvsalud.org)

There are 2 types of hematopoiesis that occur in humans: Primitive hematopoiesis - blood stem cells differentiate into only a few specialized blood lineages (typically isolated to early fetal development). (wikipedia.org)

How is the tissue specificity of immune cells generated and how is it affected by environmental influences, plasticity and the tissue microenvironment? (mdc-berlin.de)

Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
We previously established a direct single-cell analysis to simultaneously identify and quantify in vivo CMV-CTL clones after allo-HCT 9 , 10 . (nature.com)
To track the location and quantity of transplanted cells in recipient small animal model, we will generate hESC lines with in vivo reporter system that combines bioluminescent or PET imaging, and serum-based assay. (ca.gov)
Our in vivo tracking tools will be broadly relevant and not restricted to studying the in vivo biology of blood forming cells. (ca.gov)

Test the activities of the vectors in hematopoietic stem cells from patients with severe AT in culture that produces red blood cells. (ca.gov)
A common method of transferring new genes or gene-editing components into cells, to provide novel functions, is by transduction of engineered lentiviral or other vectors. (seattlechildrens.org)
They have identified multiple small molecules that facilitate the entry of viral vectors into mouse and human cells. (seattlechildrens.org)
This approach is widely applicable to a variety of gene carriers, including viral-based vectors and virus-like nanoparticles. (seattlechildrens.org)
Viruses, liposomes, and other vectors of all kinds are being used to usher new genes into cells, correcting faulty copies or equipping a cell to fight disease. (medscape.com)

The pioneering work of Till and McCulloch in 1961 experimentally confirmed the development of blood cells from a single precursor hematopoietic stem cell (HSC), creating the framework for the field of hematopoiesis to be studied over the following decades. (wikipedia.org)
Blood formation, also called hematopoiesis, is regulated by a complex system of stem cells. (idw-online.de)

Our goal is to generate a toolbox of human embryonic stem cell lines to monitor blood forming (hematopoietic) stem cell development in culture. (ca.gov)
To help overcome these bottlenecks, we will generate a suite of human embryonic stem cell reporter lines that can be used to monitor key milestones in blood stem cell development. (ca.gov)

Identification of Gene Expression-Based Prognostic Markers in the Hematopoietic Stem Cells of Patients With Myelodysplastic Syndromes. (stembook.org)
However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
Cells with surface markers of long term HSC increased the expression of a group of TFs expressed highly in normal cycling MPP1 cells. (nih.gov)

The CLP then goes on to differentiate into more committed lymphoid precursor cells. (wikipedia.org)
Most lineage specific TFs were stochastically expressed in the early precursor cells, but a few, such as Klf1, were detected only at very low levels in few precursor cells. (nih.gov)

We have previously developed a single lentiviral vector expressing a stem cell cassett, named STEMCCA, capable of generating iPS cells from post-natal fibroblasts and peripheral blood with very high efficiency that became the industry standard. (bu.edu)

As these cells are short-lived, there needs to be a steady turnover of new blood cells and the maintenance of an HSC pool. (wikipedia.org)
As the embryo requires rapid oxygenation due to its high mitotic activity, these islands are the main source of red blood cell (RBC) production via fusing endothelial cells (ECs) with the developing embryonic circulation. (wikipedia.org)
However, Ueno and Weissman provided the earliest contradiction to the hemangioblast theory when they saw that distinct ES cells mixed into a blastocyst resulted in more than 1 ES cell contributing to the majority of the blood islands found in the resultant embryo. (wikipedia.org)
DESCRIPTION (provided by applicant): In the past years, we have shown that human umbilical cord blood cells (HUCBC, U-CORD- CELL ) provide cognitive recovery in animal models of neurodegenerative disease. (sbir.gov)
Babies born with SCID lack white blood cells called T cells. (primaryimmune.org)
T cells not only directly attack cells infected with viruses, bacteria, or other microorganisms, but they also cause B cells, another type of white blood cell, to produce antibodies. (primaryimmune.org)
The newborn screening blood test can show if a baby has a low number of T cells, a sign that a baby may have SCID. (primaryimmune.org)
If a child's newborn screening blood test shows low T cells, try to stay calm and remember that you are not alone. (primaryimmune.org)
Once individual lines have been validated, lines that contain multiple fluorescent reporters will be generated, and a multi factor screen will be performed to optimize conditions that induce these blood stem cell regulators. (ca.gov)
Transfusion of washed, leukocyte-poor red blood cells (RBCs) at approximately 8-15 mL RBCs per kilogram (kg) of body weight over 1-2 hours is recommended. (medscape.com)
Hematopoietic stem cells (HSC) generate all of the blood cells throughout their life-span. (haematologica.org)
Throughout one's life, the blood is constantly being replenished from blood stem cells. (idw-online.de)
Surprisingly, mice in which the gene is mutated show a reduction in the age-associated loss of function of the blood stem cells in late life, even though the gene is no longer active. (idw-online.de)
The study results show that a certain growth and metabolic activity is necessary for the undisturbed development of our blood stem cells. (idw-online.de)
However, these two processes simultaneously burn themselves into our cells as a kind of memory and then contribute to the loss of function of the blood stem cells later in life," postulates Prof. Rudolph. (idw-online.de)
2011 - 2018, ARC Stem Cells Australia program - blood theme. (edu.au)
Hematopoietic stem cells (HSC) give rise to all types of blood lineages, including red blood cells (RBC). (lu.se)
Hemoglobin is an iron-containing protein in red blood cells that transports oxygen to tissues throughout the body. (cshlpress.com)
Combining gene signatures with bioinformatics databases (connectivity mapping) and drug repurposing to target leukaemic whilst sparing normal blood stem cells. (nottingham.ac.uk)
Sickle cell disease is an inherited blood disorder. (childrenshospital.org)
Normal red blood cells are smooth and flexible discs, like the letter O, enabling them to move easily through your child's blood vessels. (childrenshospital.org)
In contrast, sickle cells are stiff, sticky, and often shaped like the letter C. Sickle cells tend to cluster together and to the lining of blood vessels, making it difficult for them to move through small blood vessels. (childrenshospital.org)
The spleen, which helps filter bacterial infections from the blood and acts as the recycle center for old red blood cells, tends to destroy the misshapen sickle cells faster than normal red blood cells. (childrenshospital.org)
Sickle cells only live for about 14 days, while normal red blood cells can live up to 120 days. (childrenshospital.org)
This leads to a chronic lack of oxygen-carrying red cells in the blood, called anemia . (childrenshospital.org)
The Global Health Initiative at Dana-Farber/Boston Children 's Cancer and Blood Disorders Center is working to improve survival for children with sickle cell disease around the world, including programs to establish newborn screening for sickle cell disease and developing research partnerships in Liberia and Haiti. (childrenshospital.org)
Researchers are comparing chemical alterations, called DNA methylation, in the body's basic building block in healthy colon, breast, brain and lymphatic cells and their cancerous counterpart to find telltale patterns that could one day be detected in the blood, urine or feces. (sciencedaily.com)
Expansion of blood cells arising from mutant HEMATOPOIETIC STEM CELLS often related to aging. (bvsalud.org)
What makes blood stem cells transform? (lu.se)
of red blood cells. (who.int)

However, as the organism ages, increased metabolic activity can also lead to functional exhaustion of hematopoietic stem cells. (idw-online.de)

We now aim at using iPS cells for disease modeling disease modeling and study their potential for regenerative medicine. (bu.edu)

A common deleted region (CDR) in both myelodysplastic syndromes (MDS) and myeloproliferative neoplasms (MPN) affects the long arm of chromosome 20 and has been predicted to harbor a tumor suppressor gene. (elifesciences.org)

Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)

Based on results showing that rapamycin also enhances viral vector entry into cells, Dr. Torbett is studying the effects of other compounds that act on the mTOR pathway. (seattlechildrens.org)

However, cellular immune reconstitution of CMV-specific cytotoxic T-cells (CMV-CTL) is generally considered to be necessary for the long-term control of CMV reactivation after allo-HCT. (nature.com)
Severe combined immune deficiency (SCID) is a life-threatening primary immunodeficiency (PI), with a combined absence of T cell and B cell function. (primaryimmune.org)
Researchers are uncovering ever more details about the complex system of organs, tissues, and different cell types that make up the human immune system. (mdc-berlin.de)
One of the research areas at the MDC is immunology and inflammation, which covers tumor immunology, DNA repair in B lymphocytes, the role of tumor stroma-immune cell interaction in cancers, the role of innate immune cells in gliomas and neurodegenerative diseases, and the development of immunotherapy strategies. (mdc-berlin.de)
A lot of people think of cancer cells turning genes on, like oncogenes, that allow them to grow faster, evade immune surveillance, that sort of thing," Dr. Robertson says. (sciencedaily.com)
Dendritic cells play a central role in the modulation of the immune response and several inflammatory diseases have been shown to have altered mDC function. (bu.edu)

Current bioinformatic pipelines for high-throughput studies like whole genome sequencing, RNA-seq, ChIP-seq, and single cell RNA-seq are limited in their ability to map repetitive elements of the genome like ribosomal DNA. (upenn.edu)

In a whole-genome sequencing in 17 Esophageal Squamous Cell Carcinoma cases and whole-exome sequencing in 71 cases, Song (2014), reported ASH1L alterations. (cancerindex.org)

The proper copying and repair of DNA to maintain the correct sequence and structure of genomes is critical to the proper function of cells, and repair and replication is frequently disrupted in cancers and targeted by cancer treatments. (birmingham.ac.uk)
We recently discovered new ways the BRCA1 gene functions which could help expand our understanding of the development of ovarian and breast cancers. (birmingham.ac.uk)
Distinctive patterns of genes turned off -- or left on -- in healthy versus cancerous cells could enable early screening for many common cancers and maybe help avoid them scientists say. (sciencedaily.com)
Because tumor cells that result from aberrant changes shed their DNA into bodily fluids, non-invasive screening for a wide range of cancers could result be another result of this initiative, Dr. Bhalla says. (sciencedaily.com)
Dr. Shi has found, for example, a single gene that is methylated in the majority of the broad group of cancers called non-Hodgkin's lymphoma. (sciencedaily.com)

Anatomy48

Organisms14

Diseases28

Chemicals and Drugs56

Analytical, Diagnostic and Therapeutic Techniques and Equipment54

Psychiatry and Psychology1

Phenomena and Processes52

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Information Science3

Named Groups3

Health Care6

Transplantation27

Progenitors9

HSCs19

Progenitor cell6

Therapy22

HSPC6

Differentiation16

Allogeneic1

Mouse hematopoietic stem1

Pluripotent stem2

Myeloid16

Regulation5

Donor4

Mutations14

Bone9

Murine1

Proteins9

Erythroid2

Genetically3

CD346

Therapies10

Precursors1

Leukemic cells2

Early hematopoietic1

Malignant2

Clonal3

Clinical8

Mutation12

Encodes4

Metabolism5

Therapeutic3

Genetic13

Developmental2

IPSC1

HSPCs1

Fetal1

Microenvironment1

Vivo4

Vectors5

Hematopoiesis2

Embryonic Stem2

Markers3

Precursor cells2

Lentiviral vector1

Blood30

Functional1

Regenerative1

Myelodysplastic Syndromes1

Vitro1

Enhances1

Immune6

Single cell RNA seq1

Genome1

Cancers5