Hematopoietic stem cell engraftment. Medical search. Frequent questions

Successful stem cell transplantation for patients with severe combined immunodeficiency (SCID) from matched family donors without conditioning results in engraftment of T lymphocytes. (nih.gov)
Full hematopoietic engraftment was reported in one case after bone marrow transplantation without conditioning for a SCID patient. (nih.gov)
Graft-versus-host disease (GVHD) remains a significant complication in patients undergoing allogeneic stem cell transplantation (SCT) using a reduced intensity conditioning regimen. (qub.ac.uk)
In order to assess the incidence and analyze reasons which cause prolongation of hospital stay in patients engrafted after peripheral blood stem cell transplantation (PBSCT), we performed this retrospective analysis. (nature.com)
Peripheral blood stem cell and bone marrow transplantation for solid tumors and lymphomas: hematologic recovery and costs. (nature.com)
However, this balance is altered during the hematopoietic recovery after Hematopoietic Stem and Progenitor Cell Transplantation . (bvsalud.org)
Transplantation efficacy can be limited by inadequate Hematopoietic Stem Cells number, poor homing, low level of engraftment, or limited self -renewal. (bvsalud.org)
Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing the bacterial gene. (nature.com)
The goal of this work is to elucidate novel molecular mechanisms that can be therapeutically targeted to regulate cell migration in a tissue-specific fashion - to inhibit it in certain contexts (e.g., inflammatory diseases and cancer metastasis) and promote it in others (e.g., stem cell transplantation and cell-based therapies). (bu.edu)
The only curative therapy is hematopoietic stem cell transplantation. (frontiersin.org)
Umbilical cord blood (UCB) is a valuable source of hematopoietic stem cells (HSCs) and potential alternative for bone marrow transplantation for patients who lack human leukocyte antigen (HLA)-matched donors. (wjgnet.com)
Hematopoietic stem cell transplantation is the only potentially curative therapy, but it is only an option for select patients. (cancernetwork.com)
We predict the time taken for mutant clones to expand within a host, as well as chimerism levels that can be expected following transplantation therapy, and the probability that a preconditioned host is reconstituted by donor cells. (uzh.ch)
She is currently Research Scientist at Stanford University, where she is developing innovative cell therapy approaches to address neuropathic lysosomal storage diseases (LSDs) using hematopoietic stem cell transplantation (HSCT) and genome editing. (stanford.edu)
Autologous hematopoietic stem cell transplantation using genome-edited cells can become a definitive therapy for hematological and non-hematological disorders with neurological involvement. (stanford.edu)
These strains show not only a high rate of human cell engraftment, but also generate well-differentiated multilineage human hematopoietic cells after human hematopoietic stem cell (HSC) transplantation. (nih.gov)
A Multicenter, Pivotal Phase 3 Study of Iomab-B Prior to Allogeneic Hematopoietic Cell Transplantation Versus Conventional Care in Older Subjects With Active, Relapsed or Refractory Acute Myeloid Leukemia (SIERRA) Jacksonville, Fla., Rochester, Minn. (mayo.edu)
There is a paucity of data examining the tolerance and outcome of hematopoietic stem cell transplantation (HSCT) for relapse after tositumomab/iodine-131 tositumomab. (cancernetwork.com)
There is a paucity of data examining the tolerance andoutcome of hematopoietic stem cell transplantation (HSCT) for relapse aftertositumomab/iodine-131 tositumomab. (cancernetwork.com)
The safety profile of venetoclax following allogeneic hematopoietic stem cell transplantation in patients with chronic lymphocytic leukemia appears to be comparable with other reports in clinical trials. (cancernetwork.com)
Hematopoietic stem cell transplantation (HSCT) is a broad term that covers the transplantation of blood progenitor/stem cells from any source. (drugdiscoverynews.com)
However, sustained engraftment of the expanded cells was not observed after transplantation, suggesting ineffective expansion of hematopoietic stem cells with long-term repopulating activity (LTR-HSCs). (confex.com)
At optimal coating densities of Delta1, the frequency of LTR-HSCs measured by limiting dilution analysis 16 weeks after transplantation into NSG mice was 4.9- and 4.2-fold higher in hypoxic cultures (1 in 1,586 CD34+ cells) compared with uncultured cells (1 in 7,706) and the normoxia group (1 in 5,090), respectively. (confex.com)
Conversely, we observed no difference in expression of the homing CXCR4 receptor between cells cultured under normoxic and hypoxic conditions, indicating that hypoxia increased the absolute numbers of LTR-HSCs but not their homing potential after transplantation. (confex.com)
Bio My research is focused on using preclinical models to develop novel therapies which improve outcomes for patients receiving allogeneic hematopoietic stem cell transplantation. (stanford.edu)
Currently, my work aims to establish strategies to reduce the risk of relapse after allogeneic hematopoietic stem cell transplantation without exacerbating graft-versus-host disease or interfering with donor stem cell engraftment. (stanford.edu)
That is part of the mechanism [of action of the agent,] and interfering with the CD6 costimulation is another way of blunting the inappropriate immune activity that we believe is part of the underlying pathology in GVHD," explained Koreth, who is the director of Translation Research and Stem Cell Transplantation and professor of medicine at Harvard Medical School. (onclive.com)
Hematopoietic stem cell transplantation is also sometimes used for solid tumors (eg, some germ cell tumors) that respond to chemotherapy. (msdmanuals.com)
Hematopoietic stem cell transplantation may be autologous (using the patient's own cells) or allogeneic (using cells from a donor). (msdmanuals.com)
Peripheral blood has largely replaced bone marrow as a source of stem cells, especially in autologous hematopoietic stem cell transplantation, because stem cell harvest is easier and neutrophil and platelet counts recover faster. (msdmanuals.com)
Umbilical cord hematopoietic stem cell transplantation has been restricted mainly to children because there are too few stem cells in umbilical cord blood for an adult. (msdmanuals.com)
There are no contraindications to autologous hematopoietic stem cell transplantation. (msdmanuals.com)
Allogeneic hematopoietic stem cell transplantation is limited mainly by lack of histocompatible donors. (msdmanuals.com)
The technique for umbilical cord hematopoietic stem cell transplantation is still in its infancy, but it is gaining interest. (msdmanuals.com)
Because cord blood contains immature stem cells, HLA matching appears less crucial than for the other types of hematopoietic stem cell transplantation. (msdmanuals.com)
For the purposes of this document, HSCT is defined of the CDC, the Infectious Diseases Society of America, as any transplantation of blood or marrow-derived he- and the American Society of Blood and Marrow Trans- matopoietic stem cells, regardless of transplant type plantation," which was published in the Morbidity and (allogeneic or autologous) or cell source (bone marrow, Mortality Weekly Report [1]. (cdc.gov)
The growing need for haematopoietic stem cell transplantation (HSCT) is reflected in the increasing number of transplants performed globally each year. (bvsalud.org)
The specific medications administered depend on the choice of therapy and whether it is supportive care only, immunosuppressive therapy, or hematopoietic cell transplantation. (medscape.com)

Cells for HSCT may be obtained from the patient himself or herself (autologous transplant) or from another person, such as a sibling or unrelated donor (allogeneic transplant) or an identical twin (syngeneic transplant). (medscape.com)
The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
Five patients received autologous HSCT: threemarrow and two peripheral blood stem cells. (cancernetwork.com)
Among the conditions HSCT can treat are: acute myeloid leukemia, acute lymphoblastic leukemia, chronic myeloid leukemia, chronic lymphocytic leukemia, myeloproliferative disorders, myelodysplastic syndromes, multiple myeloma, non-Hodgkin lymphoma, Hodgkin disease, aplastic anemia and pure red-cell aplasia-but this list is not exhaustive. (drugdiscoverynews.com)
Other groups are also working on cell therapies for the treatment of poor graft function after HSCT. (drugdiscoverynews.com)
Their ongoing Phase 2 study is evaluating whether infusion of mesenchymal stem cells (MSCs) can treat steroid-resistant acute graft-versus-host disease (GVHD) or poor graft function after HSCT. (drugdiscoverynews.com)
Opportunistic infections (OIs) are defined as any in- the infusion of hematopoietic stem cells from a donor fections that occur with increased frequency or severity into a patient who has received chemotherapy, which in HSCT patients. (cdc.gov)

All patients except two who received reduced intensity had severe mucositis.Reversible veno-occlusive disease of the liver also developed in two patients.There was one death from graft-vs-host disease and no regimen-related mortality.Engraftment was prompt, with a median recovery of absolute neutrophil count to ³ 500/µL of 12 days (range: 11-18 days). (cancernetwork.com)

HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
These severely immunodeficient animals are required to support human cell engraftment to generate multiple differentiated lineages from human hematopoietic stem cells (HSCs). (genengnews.com)
It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
However, human hematopoietic cells developed from HSCs are not always phenotypically and functionally identical to those in humans. (nih.gov)
Results from this study showed that antibodies targeting the CD117 receptor on FA hematopoietic stem cells (HSCs) enables safe and effective HCT in FA mice when combined with T-cell depleting antibodies. (fanconi.org)
Engraftment of donor cells in mouse models also showed that when specific immunosuppressant regimens are used donor HSCs have a competitive advantage over FA HSC cells. (fanconi.org)
To corroborate these findings molecularly, we performed transcriptomic analyses and found significant upregulation of a distinct HSC gene expression signature in cells cultured with Delta1 in hypoxia (Fig. A). Collectively, these data show that hypoxia supports a superior ex vivo expansion of human HSCs with LTR activity compared with normoxia at optimized densities of Delta1. (confex.com)
To clarify how hypoxia improved Notch-mediated expansion of LTR-HSCs, we performed scRNA-seq of CD34+ cells treated with Delta1 under normoxic or hypoxic conditions. (confex.com)
FLT3 ITD triggers the proliferation of the quiescent hematopoietic stem cell (HSC) pool but fails to directly transform HSCs. (biomedcentral.com)

This article contains highlights of "Guidelines for Pre- allogeneic or autologous, depending on the source of venting Opportunistic Infections among Hematopoi- the transplanted hematopoietic progenitor cells. (cdc.gov)

There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)

These Stem Cells are highly regulated to maintain homeostasis through a delicate balance between quiescence, self -renewal and differentiation. (bvsalud.org)
The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
Highly immunodeficient mice, which do not reject xenografts and support cell and tissue differentiation and growth, are indispensable for generating additional appropriate models. (nih.gov)
The inventors have developed a novel system for co-differentiation of hepatocytes and hematopoietic progenitors from human embryonic stem cells (ES cells) that mimics a late fetal liver undergoing hematopoiesis. (warf.org)
In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)
In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
Differentiation capacity into cells from all three germ layers was confirmed using the teratoma assay. (biomedcentral.com)

2018 . DOI : 10.1016/j.cell.2018.01.028. (epfl.ch)

Evaluation of serum-free culture conditions able to support the ex vivo expansion and engraftment of human hematopoietic stem cells in the human-to-sheep xenograft model. (wakehealth.edu)
Notch activation in human CD34+ hematopoietic stem/progenitor cells (HSPCs) by treatment with Delta1 ligand has enabled clinically relevant ex vivo expansion of short-term HSPCs. (confex.com)

CRL's CD34 + humanized NCG model, HuCD34-NCG, injected with umbilical cord blood-derived CD34 + cells shows robust engraftment of major human immune cells and demonstrates infiltration of T cells into tissues and xenograft tumors. (genengnews.com)

Natural estrogens enhance the engraftment of human hematopoietic stem and progenitor cells in immunodeficient mice. (bvsalud.org)
Furthermore, we identified that E2 and, more significantly, E4 doubled human hematopoietic engraftment in immunodeficient mice without altering other Hematopoietic Stem and Progenitor Cells properties. (bvsalud.org)
Finally, the impact of E4 on promoting human hematopoietic engraftment in immunodeficient mice might be mediated through the regulation of mesenchymal stromal cells in the bone marrow niche. (bvsalud.org)
Together, our data demonstrate that E4 is well tolerated and enhances human reconstitution in immunodeficient mice , directly by modulating human Hematopoietic Progenitor properties and indirectly by interacting with the bone marrow niche. (bvsalud.org)
Additionally, in collaboration with John Dick, research from Danska's lab has previously led to the discovery of a gene (SIRPalpha) which can both support and destroy the growth of human blood stem cells, and was used to enable the successful grafting of stem cells from human bone marrow into mice. (wikipedia.org)
Interleukin 4 reverses T cell proliferative unresponsiveness and prevents the onset of diabetes in nonobese diabetic mice. (wikipedia.org)
NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
these mice carry the SIRP-alpha gene similar to humans that allows the engraftment of donor cells. (genengnews.com)
Humanized NCG mice have severely impaired murine T and B cells or innate immunity provided by macrophages and NK cells. (genengnews.com)
HuCD34-NCG mice sustain humanization through day 236 post engraftment. (genengnews.com)
When Mikkola's team used the small molecules, they observed that blood stem cell self-renewal improved in general, but the cells could not maintain proper MLLT3 levels, and they also did not function as well when transplanted into mice. (medicalxpress.com)
In this review, we describe the current knowledge of human hematopoietic cells developed in these mice. (nih.gov)
NOD/LtSz-Rag1nullPfpnull mice: a new model system with increased levels of human peripheral leukocyte and hematopoietic stem-cell engraftment. (umassmed.edu)
Small hepatic progenitor cells (SHPCs) derived from these hepatocytes have shown mature metabolic functions as well as engraftment in immunodeficient mice. (warf.org)
The inventors have previously shown that such SHPCs express high levels of mature hepatic genes, perform xenobiotic metabolism at rates equal to primary human hepatocytes, and support engraftment in immunodeficient mice (P180325). (warf.org)

All GS-2 iPSC clones displayed a normal karyotype (46XX or 46XY) and were shown to express the same RAB27A gene mutation that was present in the original somatic donor cells. (biomedcentral.com)

Pre- and post-engraftment bloodstream infection rates and associated mortality in allogeneic hematopoietic stem cell transplant recipients. (uchicago.edu)

Among seven allogeneic transplants,three received matched related peripheral blood stem cells and four receivedmatched unrelated marrow (n = 3) or peripheral blood (n = 1) stem cells.Allogeneic recipients received tacrolimus and methotrexate for graft-vs-hostdisease prophylaxis. (cancernetwork.com)
Hopefully, following the recommendations made in the guidelines will reduce morbidity and mortality from opportunistic infections in hematopoietic stem cell transplant recipients. (cdc.gov)

Subset analysis of the engrafted cells using a multiparametric system enabling a combined analysis of morphology, immunophenotyping and FISH showed that both T and B lymphocytes and myeloid cells were of donor origin in two patients, while T lymphocytes and myeloid cells were of donor origin in the third. (nih.gov)
There was 93% donor T-lymphocytes, 20% donor B-lymphocytes, and 5% donor myeloid cells, indicative of some donor stem cell engraftment. (frontiersin.org)

The study achieved its primary endpoint , a statistically significant reduction in time to neutrophil engraftment, as well as all key secondary endpoints. (biospace.com)
A key milestone in a patient's recovery, neutrophil engraftment is a measure of how quickly the stem cells a patient receives in a bone marrow transplant are established and begin to make healthy new cells. (biospace.com)

Additionally, the study met key secondary endpoints related to the speed of platelet engraftment, decrease in infections and reduction in hospitalizations, all significant clinical measures in bone marrow transplant. (biospace.com)
One trial is testing whether MSCs with or without peripheral blood stem cells could treat poor graft function and delayed platelet engraftment. (drugdiscoverynews.com)

However, the use of UCB is limited by delayed engraftment and poor immune reconstitution, leading to a high rate of infection-related mortality. (wjgnet.com)
The research interests in my lab are on the development of new approaches to enhance hematopoietic stem cell engraftment and reconstitution following blood and bone marrow transplant. (dukehealth.org)

Itolizumab, a first-in-class anti-CD6 monoclonal antibody targets the CD6-activated leukocyte cell adhesion molecule (ALCAM) pathway, which modulates the activity of T cells that drive immunoinflammatory diseases, and has demonstrated early activity as a first-line therapy for patients with high-risk aGVHD in the phase 1/2 EQUATE trial (NCT03763318). (onclive.com)

We have generated a high affinity and selective humanized monoclonal IgG4 antibody, TSR-033, which binds human LAG-3 and serves as a functional antagonist, enhancing in vitro T-cell activation both in mixed lymphocyte reactions and staphylococcal enterotoxin B-driven stimulation assays. (aacrjournals.org)

CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. (nature.com)
During the initial development of the HuCD34-NCG mouse, peripheral blood samples were collected at various timepoints, along with periodic tissue sampling, to verify engraftment and sustainability. (genengnews.com)
Humanized mouse models that have received human cells or tissue transplants are extremely useful in basic and applied human disease research. (nih.gov)
Isolation and cultivation of somatic stem cells and their application to ischemic tissue. (muni.cz)
Low-power view of hematoxylin-eosin-stained bone marrow showing hypocellularity, with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)

Pluristem Therapeutics , a leading developer of placenta-based cell therapy products, has announced that it has completed development of its second major product line, called PLX-RAD. (israel21c.org)
Pluristem Therapeutics, based in Haifa, Israel, is currently recruiting patients in the United States and Israel for its Phase 1 trial of PLX-R18 cells in this unmet need. (drugdiscoverynews.com)
Although challenges remain, investment in the field has started to bear fruit, with a number of gene and cell therapeutics reaching the market in the past decade. (bvsalud.org)

Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality. (bu.edu)
The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)

In another trial, this group is evaluating whether peripheral blood stem cells combined with MSCs can treat poor graft function. (drugdiscoverynews.com)

They robustly support the engraftment of human cells, and develop functional human immune, systems making them ideal for studying immune-related therapies in a vast array of applications. (genengnews.com)
In pilot studies, CRL evaluated the antitumor effects of immune checkpoint inhibitors (CPIs) on colon epithelial carcinoma (RKO) and basal lung cell adenocarcinoma (A549) cell lines in the HuCD34-NCG model and showed significant immunomodulatory antitumor response. (genengnews.com)
However, bone marrow transplants have significant limitations: Finding a compatible bone marrow donor is not always possible, the patient's immune system may reject the foreign cells, and the number of transplanted stem cells may not be enough to successfully treat the disease. (medicalxpress.com)
In an attempt to control infection, masses of neutrophils and other immune cells continue to gather at the site of infection, forming large groups of these cells called granulomas, hence the name of the disease. (primaryimmune.org)
In a humanized mouse non-small cell lung carcinoma model, TSR-033 boosted the antitumor efficacy of PD-1 monotherapy, with a concomitant increase in immune activation. (aacrjournals.org)
Griscelli syndrome type 2 (GS-2) is a rare, autosomal recessive immune deficiency syndrome caused by a mutation in the RAB27A gene, which results in the absence of a protein involved in vesicle trafficking and consequent loss of function of in particular cytotoxic T and NK cells. (biomedcentral.com)
By doing so, it appears to switch them from a proinflammatory immune effector cell to a less inflammatory, regulatory T-cell phenotype. (onclive.com)
One concern about the procedure is that immune cells in cord blood do not have experience with the viruses responsible for latent infections, leading to a higher percentage of naive T cells and thus increased vulnerability to reactivation of cytomegalovirus or Epstein-Barr virus infection. (msdmanuals.com)

During the engraftment phase, which lasts several weeks, the healing process begins with resolution of mucositis and other acquired lesions. (medscape.com)
The company also highlighted progress with omidubicel , an advanced cell therapy with positive Phase 3 clinical data, as a potentially life-saving treatment option for patients in need of an allogeneic hematopoietic stem cell (bone marrow) transplant, and GDA-201 , a natural killer (NK) cell immunotherapy in Phase 1/2 development for patients with non-Hodgkin lymphoma (NHL). (biospace.com)
We also continue to expand our clinical pipeline with plans to submit an IND for our GDA-201 natural killer cell therapy, initiate a multi-center Phase 1/2 clinical study in NHL and continue to advance our R&D activities to pursue the development of genetically modified NAM-enabled NK cells in solid tumors. (biospace.com)
A Phase II Study of Daratumumab-Hyaluronidase for Chemotherapy-Relapsed/Refractory Minimal Residual Disease (MRD) in T Cell Acute Lymphoblastic Leukemia (T-ALL Rochester, Minn., Jacksonville, Fla. (mayo.edu)
A041501, A Phase III Trial to Evaluate the Efficacy of the Addition of Inotuzumab Ozogamicin (a Conjugated Anti-CD22 Monoclonal Antibody) to Frontline Therapy in Young Adults (Ages 18-39 Years) With Newly Diagnosed Precursor B-Cell ALL Rochester, Minn. (mayo.edu)
Investigators will assess LP-284 as a treatment for those with B-cell non-Hodgkin lymphoma in a phase 1 trial. (cancernetwork.com)

Some immunodeficient strains are defective in T and B cell functions but retain varying amounts of NK cell activity and innate immunity. (genengnews.com)

The purpose is to deliver chemotherapy, immunotherapy, and/or radiation to eliminate malignancy, prevent rejection of new stem cells, and create space for the new cells. (medscape.com)
In view of the disseminated CMV infection, the decision was made to infuse stem cells without any pre-conditioning chemotherapy. (frontiersin.org)
However, this currently requires use of irradiation and/or chemotherapy which can be extremely harmful, especially to FA patients whose cells cannot fix DNA mistakes caused by these agents. (fanconi.org)
Drugs used in chemotherapy work in different ways to stop the growth of tumor cells, either by killing the cells, by stopping them from dividing, or by stopping them from spreading. (mayo.edu)

We conclude that in spite of rapid engraftment, non-hematological toxicities and infections remain important limitations for further reduction of the length of patient hospitalization in a significant number of patients after PBSCT. (nature.com)
Chronic granulomatous disease (CGD) is a type of primary immunodeficiency (PI) in which one group of the body's white blood cells, called neutrophils, fail to make the hydrogen peroxide, bleach, and other chemicals needed to fight bacterial and fungal infections. (primaryimmune.org)
There are complications of serial transfusions of either of these cell types, and a patient needs intense prophylactic treatments to protect from infections if his or her white counts are low. (drugdiscoverynews.com)
External insults (eg, infections, radiation, drugs) may disrupt stem cell homeostasis in marrow environment, leading to altered growth. (medscape.com)

Levels of total CD3 + T cells and CD4 + and CD8 + subsets in addition to other cell populations, such as CD19 + and CD16/56 + , were evaluated as well as cells from spleen, liver, bone marrow, lung, and lysed whole blood. (genengnews.com)
The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
Blood stem cells, also known as hematopoietic stem cells , are found in the bone marrow, where they self-renew as well as differentiate to create all types of blood cells. (medicalxpress.com)
When blood stem cells are removed from the bone marrow and placed in laboratory dishes, they quickly lose their ability to self-renew, and they either die or differentiate into other blood cell types. (medicalxpress.com)
Our method, which exposes blood stem cells to the small molecules and also inserts an active MLLT3 gene, created blood stem cells that integrated well into mouse bone marrow , efficiently produced all blood cell types and maintained their self-renewing ability. (medicalxpress.com)
Chimerism analysis - testing of short tandem repeat regions to assess donor bone marrow engraftment. (ouh.nhs.uk)
Extramedullary hematopoiesis (EMH) is likely the result of abnormal trafficking of hematopoietic stem cells (HSC) from the bone marrow to organs such as the spleen, liver, and lung, causing organomegaly and sometimes organ dysfunction. (cancernetwork.com)
Most of the patients in my clinic are blood and bone marrow transplant patients and patients with plasma cell diseases such as multiple myeloma, amyloidosis, POEMS syndrome, and Waldenstrom's macroglobulinemia. (dukehealth.org)
The first area is to define the genes and molecules in the bone marrow stem cells and in the bone marrow micro-environment that regulate hematopoietic stem cell function and number. (dukehealth.org)
Hematopoietic stem cells in mammals are known to reside mostly in the bone marrow, but also transitively passage in small numbers in the blood. (uzh.ch)
During the quarter, Gamida Cell continued to advance omidubicel, the first cell therapy for bone marrow transplant to receive Breakthrough Therapy Designation from the U.S. Food and Drug Administration (FDA). (biospace.com)
Proof-of-concept studies using human genome-edited hematopoietic stem cells have been hindered by the low efficiency of engraftment of the edited cells in the bone marrow and their modest efficacy in the CNS. (stanford.edu)
Compared with sub-lethal irradiation, Busulfan conditioning enhanced the engraftment of edited CD34+ cells in the bone marrow, as well the long-term homing and survival of bone-marrow-derived cells in viscera, and in the CNS, resulting in higher transgene expression and biochemical correction in these organs. (stanford.edu)
We think we can make this procedure safe and eliminate all of the bad side effects through use of antibodies that can target specific cells of the body, and together allow safe turnover of the bone marrow and eliminate bone marrow disease in FA patients. (fanconi.org)
The clinical presentation of patients with aplastic anemia includes signs and symptoms related to the decrease in bone marrow production of hematopoietic cells. (medscape.com)
The theoretical basis for marrow failure includes primary defects in or damage to the stem cell or the marrow microenvironment. (medscape.com)

Stem cell gene expression programs influence clinical outcome in human leukemia. (wikipedia.org)
Each of those cell sources has specific advantages and disadvantages, and each has found particular clinical applications. (medscape.com)
This review describes current considerations in the use of hydroxyurea for the management of sickle cell disease in the context of clinical severity. (cdc.gov)

Although T-cell depletion (TCD) reduces the risk of GVHD after a myeloablative conditioning regimen, it is associated with an increased risk of graft failure. (qub.ac.uk)
This application might have particular relevance to ameliorate the hematopoietic recovery after myeloablative conditioning, especially when limiting numbers of Hematopoietic Stem and Progenitor Cells are available. (bvsalud.org)

Our results show that human Hematopoietic Stem and Progenitor Cell subsets express estrogen receptors , and whose signaling is activated by E2 and E4 on these cells . (bvsalud.org)
We observed enhanced progenitor cell activity within the CD34+ cell population treated with Delta1 in hypoxia, but the benefits provided by low-oxygen cultures were most notable in the primitive HSC compartment. (confex.com)

2017 . DOI : 10.1002/stem.2719. (epfl.ch)
2017 . DOI : 10.1016/j.stem.2017.07.004. (epfl.ch)

Analogous studies in a murine syngeneic tumor model using surrogate antibodies demonstrated significant synergy between LAG-3 and PD-1 blockade-combination treatment led to a marked improvement in therapeutic efficacy, increased T-cell proliferation, IFNγ production, and elicited durable immunologic memory upon tumor rechallenge. (aacrjournals.org)

Previously, Danska's research found that in children with acute lymphoblastic leukemia, at the time of diagnosis, subpopulations of leukemic cells can cause a relapse. (wikipedia.org)
Flow cytometry analysis of peripheral blood at 12 and 16 weeks post injection verified engraftment of human cell subpopulations. (genengnews.com)

Multilineage donor engraftment is possible in SCID patients even without conditioning. (nih.gov)

The cells are also being evaluated in a variety of other hematologic indications. (drugdiscoverynews.com)

Stem cell infusion is a relatively simple process that is performed at the bedside. (medscape.com)
She received a matched sibling donor unconditioned stem cell infusion at 16 months of age. (frontiersin.org)

His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)

Polymorphism in Sirpa modulates engraftment of human hematopoietic stem cells. (wikipedia.org)

As recent evidences indicate that estrogens are involved in regulating the hematopoiesis , we sought to examine whether natural estrogens ( estrone or E1, estradiol or E2, estriol or E3 and estetrol or E4) modulate human Hematopoietic Stem and Progenitor Cells . (bvsalud.org)
We found that both E2 and E4 expand human Hematopoietic Stem and Progenitor Cells . (bvsalud.org)
However, E4 was the best tolerated estrogen and promoted cell cycle of human Hematopoietic Progenitors. (bvsalud.org)
A QC screening flow cytometry panel and a more extensive T cell and myeloid panel identify the presence of human cell populations and evaluate the model's functionality. (genengnews.com)
Viable human CD45 + cells were detected in all samples. (genengnews.com)
Human T cell infiltration was observed in both carcinomas with the majority of live T cells responsive post infiltration. (genengnews.com)
UCLA scientists have discovered a link between a protein and the ability of human blood stem cells to self-renew. (medicalxpress.com)
Multiplying blood stem cells in conditions outside the human body could greatly improve treatment options for blood cancers like leukemia and for many inherited blood diseases. (medicalxpress.com)
Although we've learned a lot about the biology of these cells over the years, one key challenge has remained: making human blood stem cells self-renew in the lab," she said. (medicalxpress.com)
To uncover what makes blood stem cells self-renew in a lab, the researchers analyzed the genes that turn off as human blood stem cells lose their ability to self-renew, noting which genes turned off when blood stem cells differentiate into specific blood cells such as white or red cells. (medicalxpress.com)
Other recent studies have identified small molecules-organic compounds that are often used to create pharmaceutical drugs-that help to multiply human blood stem cells in the laboratory. (medicalxpress.com)
Improved engraftment and therapeutic efficacy by human genome-edited hematopoietic stem cells with Busulfan-based myeloablation. (stanford.edu)
Human adult mobilized CD34+ cells were cultured for 21 days under normoxia (21% O 2 ) or hypoxia (2% O 2 ) in vessels coated with optimized concentrations of Delta1. (confex.com)
Lymphocyte activation gene-3 (LAG-3) is a coinhibitory receptor associated with impaired T-cell function and is frequently coexpressed with programmed cell death protein-1 (PD-1) in the context of human cancers. (aacrjournals.org)

Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)

BOSTON--( BUSINESS WIRE )-- Gamida Cell Ltd. (Nasdaq: GMDA), an advanced cell therapy company committed to cures for blood cancers and serious blood diseases, today reported financial results for the quarter ended March 31, 2021. (biospace.com)

It could also enable scientists to produce blood stem cells from pluripotent stem cells , which have the potential to create any cell type in the body. (medicalxpress.com)
Using pluripotent stem cells, they made blood stem cell-like cells that lacked the ability to self-renew and monitored which genes were not activated. (medicalxpress.com)
Induced pluripotent stem cells (iPSC) express genes associated with pluripotency, have the capacity for infinite expansion, and can differentiate into cells from all three germ layers. (biomedcentral.com)
A potential future source of stem cells is induced pluripotent stem cells (certain cells taken from adults and reprogrammed to act like stem cells). (msdmanuals.com)

The purpose of this study is to compare relapse-free survival between participants with FLT3/ITD AML in first morphologic complete remission (CR1) who undergo hematopoietic stem cell transplant (HCT) and are randomized to receive gilteritinib or placebo beginning after the time of engraftment for a two year period. (mayo.edu)

SCID is fatal in the first 2 years of life unless the patient is treated with extremely restrictive isolation, hematopoietic stem cell transplant or therapy that replaces the abnormal gene or gene product. (lu.se)

Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)
They found that the expression of a gene called MLLT3 was closely correlated with blood stem cells' potential to self-renew and that the protein generated by the MLLT3 gene provides blood stem cells with the instructions necessary to maintain its ability to self-renew. (medicalxpress.com)
Using a viral vector-a specially modified virus that can carry genetic information to a cell's nucleus without causing a disease-the team inserted an active MLLT3 gene into blood stem cells and observed that functional blood stem cells were able to multiply in number at least twelvefold in lab dishes. (medicalxpress.com)
Dr. Pasqualina Colella is a scientist with extensive expertise in cell therapy and gene therapy approaches for the treatment of inherited diseases. (stanford.edu)
To assess the relative impact of normoxia and hypoxia on the HSC compartment, we performed gene set enrichment analysis (GSEA) of cells within HSC cluster 5 from each culture condition. (confex.com)
Gene and cell therapies present novel alternatives to disease management, offering the promise of a single treatment and a lifelong cure. (bvsalud.org)

We have therefore examined whether TCD compromises engraftment using a fludarabine-based conditioning regimen. (qub.ac.uk)
The goal of this grant was to develop a non-genotoxic antibody-based conditioning regimen that can be used to treat people with FA who are undergoing a hematopoietic cell transplant (HCT). (fanconi.org)
The primary objective of this study is to demonstrate the efficacy of Iomab-B, in conjunction with a Reduced Intensity Conditioning (RIC) regimen and protocol-specified allogeneic hematopoietic stem cell transplant (HCT), versus Conventional Care. (mayo.edu)

Dr. Elliott J. Hagedorn is a cell and developmental biologist with expertise in hematopoiesis, stem cell biology and vascular heterogeneity. (bu.edu)
If we think about the amount of blood stem cells needed to treat a patient, that's a significant number," said Mikkola, who is also a professor of molecular, cell and developmental biology in the UCLA College and a member of the UCLA Jonsson Comprehensive Cancer Center. (medicalxpress.com)
Frontiers in Cell and Developmental Biology. (kcl.ac.uk)

Anatomy46

Organisms16

Diseases29

Chemicals and Drugs53

Analytical, Diagnostic and Therapeutic Techniques and Equipment61

Psychiatry and Psychology1

Phenomena and Processes50

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science2

Named Groups3

Health Care6

Transplantation42

HSCT7

Graft vs Host1

HSCs10

Autologous1

Control of hematopoietic stem1

Differentiation9

20181

Vivo expansion2

Umbilical1

Mice15

Donor cells1

Post-engraftment1

Recipients2

Myeloid cells2

Neutrophil2

Platelet2

Reconstitution2

Leukocyte1

Vitro1

Tissue6

Therapeutics3

Macrophages2

Peripheral blood stem cells1

Immune8

Phase6

Immunodeficient1

Chemotherapy4

Infections4

Marrow16

Clinical3

Myeloablative2

Progenitor cell2

20172

Proliferation1

Subpopulations2

Multilineage1

Hematologic1

Infusion2

Microenvironment1

Modulates1

Human14

HSPCs2

20211

Pluripotent4

Relapse1

Abnormal1

Gene6

Regimen3

Biology3