Hematopoietic progenitor stem hematopoietic bone marrow transplantation. Medical search. Frequent questions

DNMT3A is a critical regulator of hematopoietic stem cell (HSC) differentiation and somatic DNMT3A mutations are frequent in hematologic malignancies and clonal hematopoiesis. (haematologica.org)
We do not yet have a clear understanding of how this metabolic activity changes during stress hematopoiesis, such as bone marrow transplantation. (elsevierpure.com)
Here, we report a critical role for the p38MAPK family isoform p38α in initiating hematopoietic stem and progenitor cell (HSPC) proliferation during stress hematopoiesis in mice. (elsevierpure.com)
gene transfer, hematopoietic stem cells, lentiviral vectors, mesenchymal stem/progenitor cells, bone marrow transplantation, hematopoiesis Introduction HIV-based lentiviral vectors (LV) were proven to be capable of transducing a broad spectrum of nondividing cells in multiple mammalian species cIAP1 Ligand-Linker Conjugates 14 [1]. (eyesoftheelephants.com)
Depending on the degree of bone marrow damage (i.e., suppression), patients who undergo cytoreductive therapy must also receive therapy to reconstitute bone marrow function (hematopoiesis). (justia.com)
Dysregulated hematopoiesis occurs in chronic myelogenous leukemia (CML), being caused at least in part by abnormalities in the hematopoietic progenitors. (embrapa.br)
In experimental settings, the ability of human HSCs to reconstitute hematopoiesis is measured by transplantation into genetically immunocompromised mice (e.g. (stemcell.com)
Secondary Genkwanin BMT studies demonstrated that a single marrow cell able to contribute to hematopoietic reconstitution in primary recipients also drives robust osteopoiesis and LT hematopoiesis in secondary recipients. (morainetownshipdems.org)
Using supplementary BMT assays we display here a solitary marrow cell in a position to donate to hematopoietic reconstitution in major recipients drives both osteopoiesis and long-term (LT) hematopoiesis in supplementary recipients. (morainetownshipdems.org)
small fraction of marrow reconstituted short-term however not LT hematopoiesis and didn't bring about osteoblasts (Shape 1f). (morainetownshipdems.org)
In 30-50% of patients, the lymphoblasts infiltrate bone marrow, causing ineffective hematopoiesis. (medscape.com)

high-risk patients included male subjects, recipients of stem cells from female donors, patients younger than 10 years, those with nonidentical donors, and those who received radiation therapy in the transplant preparative regimen. (medscape.com)
In a study from the United States, long-term survivors of pediatric bone marrow transplantation followed in the Bone Marrow Transplant Survivors Study were compared with survivors of childhood cancer treated without bone marrow transplant from the Childhood Cancer Survivor Study. (medscape.com)
Blood Marrow Transplant. (bcm.edu)
Ramos CA " Striking the right balance: optimizing conditioning regimens for unrelated donor bone marrow transplant for severe aplastic anemia. . (bcm.edu)
Bone Marrow Transplant. (musc.edu)
Blood and Marrow Transplant Research [CIBMTR] in 2004), the European Research Project on Cord Blood Transplantation (Eurocord) in 1993, and the Japanese Cord Blood Banking Network in 1996-expedited the clinical evaluation of the efficacy and safety of transplantation of cord blood from unrelated donors. (nationalacademies.org)
In the mid-20th century, Dr. E. Donnall Thomas achieved a landmark breakthrough by successfully performing the first bone marrow transplant between identical twins in 1956 (Thomas et al. (revistadehematologia.org.mx)
In April 1960, Dr. Álvaro Gómez-Leal, presented during the first meeting of the Agrupación Mexicana para el Estudio de la Hematología, A.C., data on a transplant of allogeneic stem cells in a patient with acute leukemia done in Monterrey, Mexico: the patient received high-dose chemotherapy followed by stem cells from the bone marrow of his brother, improving and obtaining remission for months but relapsing and subsequently died. (revistadehematologia.org.mx)
It was only until 1988 when the same group could report on their first successful bone marrow transplant (León-Rodríguez et al. (revistadehematologia.org.mx)
PHILADELPHIA (February 8, 2017) - The Fox Chase-Temple University Hospital Bone Marrow Transplant Program has received internationally-recognized reaccreditation by the Foundation for the Accreditation of Cellular Therapy (FACT) at the University of Nebraska Medical Center. (foxchase.org)
FACT is an internationally-recognized accrediting body for hospitals and medical institutions offering stem cell transplant, and indicates the accredited institution has met the most rigorous standards in every aspect of stem cell therapy. (foxchase.org)
The bone marrow transplant program has been found to be in compliance with these rigorous Standards as well as governmental regulations. (foxchase.org)
Hopefully, following the recommendations made in the guidelines will reduce morbidity and mortality from opportunistic infections in hematopoietic stem cell transplant recipients. (cdc.gov)
For the purposes of this document, HSCT is defined of the CDC, the Infectious Diseases Society of America, as any transplantation of blood or marrow-derived he- and the American Society of Blood and Marrow Trans- matopoietic stem cells, regardless of transplant type plantation," which was published in the Morbidity and (allogeneic or autologous) or cell source (bone marrow, Mortality Weekly Report [1]. (cdc.gov)

In addition to complications seen from exposure to chemotherapy and radiation, patients undergoing allogeneic transplantation can experience unique late effects secondary to graft versus host disease (GVHD) and autoimmunity. (medscape.com)
Stem cell transplantation is performed after high-dose chemotherapy (HDC) to restore a cancer patient's blood and immune cell production capacity. (bdbiosciences.com)
3 Following exogenous stimulation, such as chemotherapy or using growth factors such as granulocyte colony stimulating factor (G-CSF) and filgrastim, the number of HSCs in the peripheral blood increases, either becoming on par or even exceeding the number in the bone marrow. (bdbiosciences.com)
For many hematopoietic malignancies, collection and infusion of CD34+ hematopoietic stem/progenitor cells following chemotherapy is critical. (bdbiosciences.com)
This is defined by their ability to repopulate an individual whose hematopoietic system has been destroyed by radiation or chemotherapy. (justia.com)
After harvesting, the patient is given high dose chemotherapy or radiotherapy and the bone marrow function is reconstituted by infusion of the cells harvested earlier. (justia.com)
The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
Purpose: To determine the tolerability and feasibility of double-cycle, high-dose chemotherapy followed by peripheral blood stem-cell transplantation (PBSCT) after conventional chemotherapy or chemoradiotherapy for small cell lung cancer (SCLC). (the-medical-dictionary.com)
Once prepared, the haematopoietic progenitors are infused back into the patient after an intensification process with high doses of chemotherapy . (mdanderson.es)
After applying high doses of chemotherapy, we rescue the person with his or her own cells to repopulate his or her bone marrow and start the normal production of red blood cells, white blood cells and platelets again ," states the doctor. (mdanderson.es)
In autologous transplantation the tool is chemotherapy, in allogeneic transplantation it is the new immune system and in CAR-T cell therapy it is the patient's own re-educated T-lymphocytes ," concludes this expert. (mdanderson.es)
Preconditioning of recipients by lethal irradiation or chemotherapy forces transplanted hematopoietic stem and progenitor cells to realize their maximum potential, but this does not necessarily reflect their fate and behavior in the native bone marrow environment. (digs-bb.de)
Because of the patient's risk status, the physicians intended to perform allogeneic stem cell transplantation after induction and consolidation chemotherapy, which was scheduled to end in January 2013, and a conditioning chemotherapy regimen, which was planned to be given in March. (cdc.gov)
Opportunistic infections (OIs) are defined as any in- the infusion of hematopoietic stem cells from a donor fections that occur with increased frequency or severity into a patient who has received chemotherapy, which in HSCT patients. (cdc.gov)
Treatment includes induction chemotherapy to achieve remission and postremission chemotherapy (with or without stem cell transplantation) to avoid relapse. (msdmanuals.com)

2 HSCs are primarily found in bone marrow niches, but a small fraction of HSCs can also be found in the peripheral blood. (bdbiosciences.com)
This pharmacologically induced egress of HSCs into peripheral blood, called mobilization, is utilized as the preferred strategy for generating HSCs for transplantation. (bdbiosciences.com)
4 CD34 expression is historically related to hematopoietic cells and it is considered as the marker of HSCs. (bdbiosciences.com)
Flow cytometric enumeration of CD34+ HSCs and progenitor cells is an established method for the evaluation of bone marrow and stem cell grafts. (bdbiosciences.com)
Hematopoietic stem cells (HSCs) are a type of multipotent stem cell found primarily in the bone marrow and, to a lesser extent, in peripheral blood. (thenoveldifference.com)
Hematopoietic stem cells (HSCs) maintain quiescence by activating specific metabolic pathways, including glycolysis. (elsevierpure.com)
Herein, we demonstrated the biological effects and mechanisms of asymmetrical loss in hematopoietic stem cells (HSCs). (bvsalud.org)
RESULTS: Atp8a1 deletion resulted in slight hyperleukocytosis, associated with the high proliferation of HSCs and BCR/ABL1 transformed leukemia stem cells (LSCs). (bvsalud.org)
Using inducible deletion of autophagy specifically in adult hematopoietic stem cells (HSCs) and in mice chimeric for autophagy-deficient and normal HSCs, we demonstrate that the stem cell loss is cell-intrinsic. (ox.ac.uk)
Hematopoietic stem cells (HSCs) are at the top of the hematopoietic hierarchy and can differentiate into all types of mature blood cells throughout life. (digs-bb.de)
HSCs were first identified by transplantation, in which recipient mice were preconditioned with ionizing radiation and reconstituted with donor bone marrow. (digs-bb.de)
We recently unraveled an alternative pathway of thrombopoiesis, in which HSCs directly differentiate into megakaryocytes, bypassing the classical multipotent progenitor intermediates (Morcos, Li, Munz et al. (digs-bb.de)
We hypothesize that the continuous but rare differentiation of quiescent HSCs into rapidly dividing progenitors limits mutation accumulation in the hematopoietic system. (digs-bb.de)
Hematopoietic stem cells (HSCs) enable hematopoietic stem cell transplantation (HCT) through their ability to replenish the entire blood system. (lu.se)
This regulation becomes important in the transplantation setting where HSCs undergo proliferation followed by a gradual transition to quiescence and homeostasis. (lu.se)
We found that regenerating HSCs initially produced rapidly expanding progenitors and displayed distinct changes in fatty acid metabolism and glycolysis. (lu.se)
Moreover, we observed molecular changes in cell cycle, MYC and mTOR signaling in both HSCs, and progenitor subsets. (lu.se)
We used a decay rate model to fit the temporal transcription profiles of regenerating HSCs and identified genes with progressively decreased or increased expression after transplantation. (lu.se)
Hematopoietic stem cells (HSCs) are very rare cells, representing approximately one in 100,000 bone marrow (BM) cells in the adult. (longdom.org)
HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)

They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
Progenitor cells, also known as precursor cells, are a type of cell that falls somewhere between stem cells and fully differentiated, specialized cells in terms of their differentiation potential. (thenoveldifference.com)
They have already committed to a particular differentiation pathway, meaning they are destined to become a specific type of cell, such as a red blood cell, white blood cell, or platelet in the case of hematopoietic progenitor cells. (thenoveldifference.com)
This reduced differentiation of CD34+ hematopoietic progenitor stem cells could occur due to both infectious and non-communicable diseases and also due to their drug induced treatments. (hoajonline.com)
Retention of cellular phenotype is the hallmark of differentiation of pluripotent stem cells. (hoajonline.com)
We found that many bone marrow stromal cells have the ability of multi-lineage differentiation potential and CFU-F activity in vitro, but failed to form new bone when transplanted in vivo. (bjmu.edu.cn)
B lood cell differentiation begins with multipotent hematopoietic progenitor cells (HPCs), which are located in the marrow spaces of the bone. (nationalacademies.org)
To investigate the proliferation and differentiation of hematopoietic stem and progenitor cells in their physiologic environment, we use state-of-the-art mouse models. (digs-bb.de)
Mesenchymal stem cells (MSC) represent a population of the bone marrow microenvironment, which participates in the regulation of haematopoietic stem cells (HSC) self-renewal and differentiation. (staminafoundation.org)
The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
A knowledge from the systems that regulate this differentiation potential can lead to book remedies for disorders of bone tissue aswell as options for conserving the integrity of endosteal hematopoietic niches. (morainetownshipdems.org)
The proliferation and differentiation of pluripotent stem cells give rise to progeny that can populate the entire immunologic and hematopoietic systems through committed progenitors of both the lymphoid and myeloid lineages. (medscape.com)
8,9 Because dental stem cells (DSCs) are easy to obtain and present a great potential of differentiation, there has been a growing interest in their use in regenerative medicine for treatment of various human diseases. (bvsalud.org)

While progenitor cells can divide and replicate, their proliferation potential is more restricted compared to stem cells. (thenoveldifference.com)
PMF is characterized by proliferation predominantly of bone marrow megakaryocytes and granulocytes, resulting in deposition of fibrous connective tissue, and often associated with peripheral blood leukoerythroblastosis, cytopenias, and constitutional symptoms. (oncohemakey.com)
ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
In acute myeloid leukemia (AML), malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived myeloid progenitor cell results in high circulating numbers of immature blood cells and replacement of normal marrow by malignant cells. (msdmanuals.com)

Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)

The presence of CD34 on non-hematopoietic cells in various tissues has been linked to progenitor and adult stem cell phenotypes. (wikipedia.org)
But research progressed and different trials explore the clinical potential of human MSCs isolated from bone marrow but also from other tissues including adipose tissue. (hindawi.com)
Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
Progenitor cells are often located within specific tissues, close to where they will ultimately function. (thenoveldifference.com)
In addition cIAP1 Ligand-Linker Conjugates 14 to HSC, bone marrow contains mesenchymal stem/progenitor cells (MSC), which can differentiate into mature cells of multiple mesenchymal tissues cIAP1 Ligand-Linker Conjugates 14 including fat, bone, and cartilage. (eyesoftheelephants.com)
The subject's blood can be processed and used to repopulate the destroyed lymphohematopoietic system of a recipient and may in the future be utilized to repair a variety of non-hematopoietic tissues. (justia.com)
In vivo transplantation demonstrated that LIFR+PDGFRB+CD45-CD31-CD235a- MSCs could form bone tissues and reconstitute the hematopoietic microenvironment (HME) effectively in vivo. (bjmu.edu.cn)
Multipotent mesenchymal stromal cells (MSCs) were first isolated from bone marrow and then from various adult tissues including placenta, cord blood, deciduous teeth, and amniotic fluid. (embrapa.br)
It is thus essential when designing clinical approaches that use tissues or cells derived from human embryonic stem cells (hESC), to specifically target the production of stem and progenitors that will survive, proliferate and differentiate normally after transplantation. (ca.gov)
Nonetheless, the transplantation of organs and tissues does raise ethical concerns. (who.int)
Successful transplantation of organs and living tissues depends on continued medical follow-up and the patient's compliance with a regimen of immunosuppressive drugs. (who.int)
Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)
Due to the ability to self-renew and to differentiate into cells that are found throughout the body, there is a great interest in using stem cells for the regeneration of injured tissues as well as to develop tissue-engineered implants and bio-hybrid organs, in order to restore tissue function. (bvsalud.org)
MSCs can be isolated from different locations, such as bone marrow, umbilical cord, placenta, adipose and dental tissues. (bvsalud.org)
In human postnatal dental tissues, five main sources of DSCs have been identified: dental pulp stem cells (DPSCs), 11 stem cells from human exfoliated deciduous teeth (SHEDs), 12 periodontal ligament stem cells (PDLSCs), 13 dental follicle stem cells (DFSCs) 14 and stem cells from apical papilla (SCAPs). (bvsalud.org)

Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)

Gene delivery through stem cells using specific viral vectors are also utilised to confer lineage specificity of transducedautologous or allogeneic stem cell gene expressionin the recipient while addressing graft versus host disease. (hoajonline.com)
Although MSC immunomodulating properties are not yet completely understood, their low immunogenic potential can be used as a therapeutic tool not only for regenerative medicine, but also for the treatment of graft-versus-host disease (GVHD) after bone marrow transplantation as well as for specific cases of severe refractory autoimmune diseases. (staminafoundation.org)
The allogeneic HSCT was the most frequently performed (57.14%) and the most used source of Hematopoietic progenitor cells (HPC) was the peripheral blood (54.29%) and 5.71% of these patients developed the Graft versus Host Disease (GVHD), of which one was affected by acute GVHD and another by chronic GVHD. (bvsalud.org)

Autologous hematopoietic stem cell transplantation and reconstitution: Different species and including primates and humans for studies are being used for development, localization, and therapeutic strategies. (hoajonline.com)
Since the anti-CD34 monoclonal antibody 12-8 reacts with a similar marrow population in baboons, it was possible to test whether this antigen is expressed by stem cells responsible for hematopoietic reconstitution in vivo. (nih.gov)
The data suggest that stem cells responsible for hematopoietic reconstitution are CD34+. (nih.gov)
The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
MTOR inhibition by rapamycin treatment in vivo was able to rescue autophagy-deficient HSC loss and bone marrow failure and resulted in better reconstitution after transplantation. (ox.ac.uk)
We isolated bone marrow from mice at 8 time points after transplantation and examined the reconstitution dynamics and transcriptional profiles of stem and progenitor populations. (lu.se)

However, it has become apparent more recently that the types of blood cells that hESC can produce under current conditions are more limited functionally than those found in bone marrow or cord blood. (ca.gov)

Likewise, many children are now surviving hematopoietic stem-cell transplantation (HSCT) (see related histology slide below) and require structured long-term follow-up care. (medscape.com)
30,000 patients with blood-related malignancies receive HDC, which, if the response is satisfactory, could subsequently be followed by hematopoietic stem cell transplantation (HSCT). (bdbiosciences.com)
This was the first report of a hematopoietic stem cell transplantation (HSCT) conducted in Mexico, only 4 years after the pioneer work by E. Donnall Thomas in Cooperstown, New York, USA (Ruiz-Argüelles et al. (revistadehematologia.org.mx)
Since then, allogeneic hematopoietic stem cell transplantation (HSCT) has evolved to become a frequently used and effective therapy for many hematologic malignancies. (longdom.org)
The Hematopoietic stem cell transplantation (HSCT) is used in children as a definitive treatment for various oncological, immune deficiencies, hemoglobinopathy, and malignancies diseases that involve the hematological system, congenital metabolism disorders, among others. (bvsalud.org)
This is a quantitative, retrospective, observational, descriptive and analytical quantitative approach approaching the medical records of children and adolescents submitted to HSCT in a referral hospital service for this type of transplantation in the state of Rio Grande do Sul North (RN). (bvsalud.org)
Hematopoietic stem cell transplantation not responded to other therapeutic modalities, with (HSCT) is used in children as a definitive treatment an increase in survival after transplantation, for different oncological, immune deficiencies, contributing to its use (YEILIPEK, 2014). (bvsalud.org)
are basically 3 phases of immune recovery for HSCT patients, The purposes of the guidelines are (1) to summarize the beginning at day 0, the day of transplantation. (cdc.gov)

As the cells reproduce, they commit to a particular task or cell line and become known as committed progenitor cells . (nationalacademies.org)
These committed progenitor cells are difficult to discern from the original multipotent cells but can be cultured to form colonies of specific types of blood cells (Guyton and Hall, 2000). (nationalacademies.org)
Umbilical cord blood is a rich source of these committed progenitor cells and, presumably, multipotent HPCs (Knudtzon, 1974). (nationalacademies.org)

Hematopoietic stem cells give rise to progenitor cells, which, in turn, differentiate into specific blood cell lineages. (thenoveldifference.com)
Embryonic stem cells which are characteristic of totipotency differentiate into generations of the multiple organs. (hoajonline.com)
mesenchymal stem cells - placenta) differentiate into multipotency as precursors to formation of the cellular components. (hoajonline.com)
1992). Since then, hematopoietic stem cells, with their unique ability to differentiate into various blood cell types, have proven to be a revolutionary tool in the treatment of numerous hematological disorders. (revistadehematologia.org.mx)
Bone marrow is the place for the production of the hematopoietic cells which differentiate in to three groups: red blood cells, white blood cells and platelets. (longdom.org)
Stem cells (SCs) are undifferentiated cells with self-renewal ability and capacity to differentiate into specialized cell types. (bvsalud.org)

Association of stromal cell-derived factor-1-3'A polymorphism to higher mobilization of hematopoietic stem cells CD34+ in Tunisian population. (cdc.gov)

The hematopoietic retrodifferentiated stem cells have been shown to engraft an animal host in two proofs of principle clinical studies, demonstrating long-term engraftment and safety in acquired aplastic anaemia, while transient amelioration of beta thalassemia major was also observed. (eurekaselect.com)
Kamble RT, Guo S, Ramos CA, Carrum G " Acute gout at engraftment following hematopoietic transplantation. . (bcm.edu)
Three months after osteoblast engraftment (1.5-2.0% donor cells), representative specimens of trabecular bone showed histologic changes indicative of new dense bone formation. (nih.gov)
Thus, allogeneic bone marrow transplantation can lead to engraftment of functional mesenchymal progenitor cells, indicating the feasibility of this strategy in the treatment of osteogenesis imperfecta and perhaps other mesenchymal stem cell disorders as well. (nih.gov)
NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)

In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)

CD34 was first described on hematopoietic stem cells independently by Civin et al. (wikipedia.org)
The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early haematopoietic and vascular-associated progenitor cells. (wikipedia.org)
CD34 is expressed in roughly 20% of murine haematopoietic stem cells, and can be stimulated and reversed. (wikipedia.org)
2 Evaluation of harvest adequacy requires the use of reliable progenitor cell assays and this is usually achieved by CD34 cell counting using flow cytometry. (bdbiosciences.com)
CD34, a transmembrane phosphoglycoprotein, is present on immature hematopoietic precursor cells and all hematopoietic colony-forming cells in bone marrow and blood, including unipotent and pluripotent progenitor cells. (bdbiosciences.com)
An accurate measurement of CD34 is critical for dose requirement protocols in stem cell transplantation. (bdbiosciences.com)
The BD ® Stem Cell Enumeration (SCE) Kit provides simultaneous enumeration of viable dual-positive CD45+/CD34+ hematopoietic stem cell populations in CD34+ absolute counts (cells/µL) as well as the percentage of the total viable leucocyte count that is CD34+ (%CD34). (bdbiosciences.com)
The CD34 antigen is present on 1-4% of human marrow cells including virtually all hematopoietic progenitors detected by in vitro assays. (nih.gov)
Moreover, in human studies, JAK2V617F has been identified in the stem cell-enriched CD34 + CD38 − CD90 + Lin − compartment, common myeloid progenitors, granulocyte-monocyte precursors, and megakaryocyte-erythroid precursors as well as natural killer cells, B cells, and T cells in some patients, confirming its origin in an early hematopoietic progenitor. (oncohemakey.com)
The CXCL12-3'A allele is associated with a higher mobilization yield of CD34 progenitors to the peripheral blood of healthy donors for allogeneic transplantation. (cdc.gov)

The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
Myocardial infarctions were generated in mice and different cell populations including transgenic MSCs, unfractionated BM cells, or purified hematopoietic progenitors were injected. (ox.ac.uk)
Following intravenous administration of a first-generation LV into adult mice, we found that bone marrow exhibited the highest levels of transgene among nine organs examined, with more than 10% green fluorescent protein-positive (GFP+) cells detected in peripheral blood leukocytes (PBL) in these mice [9]. (eyesoftheelephants.com)
It was also observed by others that a significant transgene signal was detected in the bone marrow (BM) by PCR analysis in adult mice of systemic administration of HIV-biased LV [10]. (eyesoftheelephants.com)
Recently, we exhibited detectable levels of transgene (up to 3.9%) in PBL of mice 4 months after secondary bone marrow transplantation (BMT) of HSC transduced by delivery of LV in newborn pups [11]. (eyesoftheelephants.com)
In this study, all parameters governing these processes were estimated in a computational model to describe the haematopoietic hierarchy in adult mice. (lu.se)
We also demonstrate that C3AR in combination with GPR56 distinguishes the leukemic stem cells (LSCs) in NPM1-mutated AML from the normal hematopoietic stem cells, defining the LSC population, as shown by transplantation into immunodeficient mice. (lu.se)

Progenitor cells are being explored for various therapeutic applications, including tissue engineering and regenerative medicine. (thenoveldifference.com)

Changes in the sources and handling of hematopoietic stem cells were introduced. (revistadehematologia.org.mx)

It may also mediate the attachment of hematopoietic stem cells to bone marrow extracellular matrix or directly to stromal cells. (wikipedia.org)
However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
Overview of studies published regarding MSCs using the term "stem cell" or "stromal cell" in the last 20 years accessed by a web search in July 2015 (Google Scholar). (hindawi.com)
Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)

Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. (lecturio.com)
We have called this primitive population "embryonic mesoderm progenitor" (EMP) cells as they have the potential to make many if not all the cells of the mesoderm germ layer. (ca.gov)
however the source of this activity whether a primitive osteoprogenitor or a transplantable marrow cell with dual hematopoietic and osteogenic potential has eluded detection. (morainetownshipdems.org)
Comparing the cell surface expression on NPM1-mutated AML cells to primitive (CD34+ CD38-) normal bone marrow cells, we identified the complement receptor C3AR as specifically expressed in NPM1-mutated AML. (lu.se)

Our data provide evidence that adult stem cells in bone marrow can be efficiently transduced vector administration without preconditioning. (eyesoftheelephants.com)
Adult stem cells are long-lived and quiescent with unique metabolic requirements. (ox.ac.uk)
a broad range of disorders for which transplantion of HPCs from an adult donor is also successful, including hematological malignancies, solid tumors, constitutional and acquired bone marrow failure syndromes, hemoglobinopathies, congenital immune deficiencies, and inherited disorders of metabolism (Gluckman et al. (nationalacademies.org)
By demonstrating compliance with the FACT-JACIE International Standards for Cellular Therapy Product Collection, Processing and Administration , the joint program of Fox Chase Cancer Center and Temple has earned FACT reaccreditation for adult allogeneic and autologous hematopoietic progenitor cell transplantation, peripheral blood cellular therapy product collection, and cellular therapy product processing with minimal manipulation. (foxchase.org)
It is well recognized from adult stem cell studies that the growth of transplanted bone marrow is generated from the hematopoietic ("blood-forming") stem and progenitor cells provided by the donor bone marrow. (ca.gov)
1,2 Regarding the origin, they can be classified as embryonic stem cells (ESCs) and adult stem cells (ASCs). (bvsalud.org)

Progenitor cells play a crucial role in tissue repair and regeneration. (thenoveldifference.com)
Therapeutic regeneration of molecularly engineered and transplanted autologous stem cells in neurological and cardiovascular diseases due to inability of self-regeneration or repair of endogenous stem cells at sites of inflammation or disease at various stages of progress for regeneration of the damaged tissue depending upon the sites and nature of degeneration. (hoajonline.com)
The best hope for regeneration of these permanently damaged cells is optic stem cell therapy combined with continued drug treatments or other exogenous biomaterial implants depending upon the relevant ophthalmological condition. (hoajonline.com)
During limb epimorphic regeneration: committed mesenchymal cells at the stump site dedifferentiate, forming a cluster of heterogeneous population of stem cells, known as the blastema. (eurekaselect.com)
This work not only expands our understanding of human bone marrow MSCs, but also provides great hints for further expansion of primary MSCs in vitro, which would be great help for MSCs-based bone regeneration. (bjmu.edu.cn)
Progenitor cell therapy describes the use of multipotent cells of various cell lineages (autologous or allogeneic) for tissue repair and/or regeneration. (southcarolinablues.com)
1 Treatment with progenitor cells (i.e., stem cells) offers potential benefits beyond those of standard medical care, including the potential for repair and/or regeneration of damaged myocardium. (southcarolinablues.com)
We want to understand how the hierarchical organization of mature blood cell regeneration from stem and progenitor cells shapes the accumulation of somatic mutations and hence the risk of developing blood cancer. (digs-bb.de)
Here, we analyzed the different phases of regeneration after transplantation. (lu.se)
MSC are multipotent non-haematopoietic progenitors, which have been explored as a promising treatment in tissue regeneration. (staminafoundation.org)
These cells have been studied for the treatment of periodontitis, bone repair, regeneration of the pulp after necrosis as well as the development of new teeth. (bvsalud.org)

The inventors have identified a way to use bone marrow derived endothelial progenitor cells for use in cell-based therapy ranging from reparative medicine to drug screening. (univation.co)
Endothelial Progenitor cells (EPC) express a subject of integrins and fibronectin, proteins that make them adhesion-competent cells. (univation.co)
The microenvironment, consisting of lymphocytes, macrophages, fibroblasts, endothelial elements, and stroma in which hematopoietic cells reside, creates a regulatory niche that determines the local area network. (medscape.com)

Globally, it is estimated that 120 000 corneal transplantations and 18 000 transplantations of allogeneic haematopoietic progenitor cells took place in the year 2000. (who.int)

Additionally, competitive bone marrow transplantation and 5-FU stress assays were performed. (bvsalud.org)
For decades, hematopoietic stem and progenitor cells have been studied primarily through such transplantation-based assays. (digs-bb.de)

This experimental paradigm revealed the existence of a rare population of somatic stem cells that can stably reconstitute all blood lineages lifelong. (digs-bb.de)
These may be classified into those that result in pancytopenia and those limited to failure of 1 or 2 hematopoietic lineages. (medscape.com)
All blood cells originate from a population of relatively few haematopoietic stem cells residing in the bone marrow, which give rise to specific progenitors through different lineages. (lu.se)

Phenotypic analysis of early hematopoietic progenitors in cord blood and determination of their correlation with clonogenic progenitors: relevance to cord blood stem cell transplantation. (musc.edu)

Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. (wikipedia.org)
Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)
No obvious correlations between clinical parameters, including age, karyotype, existence of paroxysmal nocturnal hemoglobinuria clones, pretreatment blood counts, progenitor cell counts, and the response to immunosuppressive therapy (IST), were found. (springermedizin.at)
The ease by which various stem cell types can be generated from human peripheral blood has allowed the design of various kits to guarantee the specificity, sterility and efficacy of stem cells production for various clinical and research applications. (eurekaselect.com)
In principle, transplantation of mesenchymal progenitor cells would attenuate or possibly correct genetic disorders of bone, cartilage and muscle, but clinical support for this concept is lacking. (nih.gov)
Although these disorders share certain clinical features-including bone marrow hypercellularity, frequent splenomegaly, and risks of thrombosis, hemorrhage, and transformation to acute myeloid leukemia (AML)-they also show important phenotypic differences. (oncohemakey.com)
Overall, this evidence has suggested that progenitor cell treatment may be a promising intervention, but robust data on clinical outcomes are lacking. (southcarolinablues.com)
Experimental and clinical data gave encouraging results, showing that MSC injection allowed controlling refractory GVHD, restoring bone development in children with osteogenesis imperfecta or improving heart function after myocardial infarction. (staminafoundation.org)
It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
Donor CTLA-4 genotype influences clinical outcome after T cell-depleted allogeneic hematopoietic stem cell transplantation from HLA-identical sibling donors. (cdc.gov)
This computational approach to the haematopoietic system is novel and provides insight into the dynamics and the nature of possible solutions, with potential applications in both fundamental and clinical research. (lu.se)

In order to assess the incidence and analyze reasons which cause prolongation of hospital stay in patients engrafted after peripheral blood stem cell transplantation (PBSCT), we performed this retrospective analysis. (nature.com)

Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. (wikipedia.org)
Hematopoietic stem cell transplantation is replacing the old fashioned bone marrow transplants. (univation.co)
Firstly, precisely these haematopoietic progenitors are key in autologous and allogeneic transplants, but not in CAR-T therapies. (mdanderson.es)
At the time they performed the first transplants surprisingly little was known about hematopoietic stem cells, immune responses to transplants or the complex human leucocyte antigen system. (revistadehematologia.org.mx)
The number of human tissue transplants is increasing in both developed and developing countries, but global data on this form of transplantation are less complete. (who.int)
Access to transplantation is limited in low- and many medium-income countries, where the rate of transplants remains far below that of richer nations. (who.int)

It seems that the term "stem cell" became more popular although the "stemness" was only shown in a more strict sense for MSCs involved in osteogenesis and bone repair. (hindawi.com)
When the biological properties of MSCs were explored in more detail, questions arose whether these cells met the criterion of a true stem cell [ 8 ]. (hindawi.com)
Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
The aim of our study was to determine the fate of injected mesenchymal stem cells (MSCs) and whole bone marrow (BM) cells in the infarcted heart. (ox.ac.uk)
While the common cell surface markers such as CD146, CD271 and PDGFRa used for isolating MSCs were not detected, LIFR+PDGFRB+ were identified to be specific markers of MSCs as the early progenitors. (bjmu.edu.cn)
However, the role of mesenchymal stem cells (MSCs) in CML has not been well characterized. (embrapa.br)
The objectives of the present study were to observe the biological characteristics of MSCs from CML patients and to determine if MSCs originate in part from donors in CML patients after bone marrow transplantation (BMT). (embrapa.br)
Mesenchymal stem cells (MSCs) are ASCs, and were first described in 1966 by Friedenstein et al. (bvsalud.org)

However, counting CD34+ mononuclear cells may overestimate myeloid blasts in bone marrow smears due to hematogones (B lymphocyte precursors) and CD34+ megakaryocytes. (wikipedia.org)
Influence of molecular subgroups on outcome of acute myeloid leukemia with normal karyotype in 141 patients undergoing salvage allogeneic stem cell transplantation in primary induction failure or beyond first relapse. (cdc.gov)
Bone marrow failure syndrome (BMFS) is a group of disorders that may manifest as single cytopenia (eg, erythroid, myeloid, or megakaryocytic) or as pancytopenia. (medscape.com)

Injection of CD34+ hematopoietic stem cells has been clinically applied to treat various diseases including spinal cord injury, liver cirrhosis and peripheral vascular disease. (wikipedia.org)
In the last decade, the number of transplantations of HPCs derived from cord blood has increased, particularly for children. (nationalacademies.org)
2003). After the early success of transplantation of cord blood from related donors, cord blood banks were established to provide rapidly accessible, human leukocyte antigen (HLA)-typed units predominantly for transplantation of HPCs from unrelated donors. (nationalacademies.org)
1987). Umbilical cord blood was recognized as an alternative source of hematopoietic stem cells (Gluckman et al. (revistadehematologia.org.mx)
1 This includes human cells for transplantation such as haematopoietic stem cells from bone marrow, peripheral blood or cord blood. (who.int)

Hematopoietic stem cells are widely used in medical treatments, particularly in bone marrow transplantation for individuals with hematological disorders like leukemia or aplastic anemia. (thenoveldifference.com)
Bone marrow section from a 7-year-old girl with idiopathic acquired aplastic anemia. (medscape.com)
The etiology of bone marrow failure (BMF) includes defective stem/progenitor cells and/or stroma/accessory cells/growth factors, as well as deficient nonspecific nutrients or, as in the case of acquired aplastic anemia, immune-mediated abnormalities. (medscape.com)

Tumors contain a minority population of cancer stem cells that maintain the tumor. (hoajonline.com)
In marked contrast to the cancer stem cells, the tumor cells without "stemness" have either no capacity or a markedly diminished capacity, to form new tumors. (hoajonline.com)
Peripheral blood stem cell and bone marrow transplantation for solid tumors and lymphomas: hematologic recovery and costs. (nature.com)

Here we describe the initial results of allogeneic bone marrow transplantation in three children with osteogenesis imperfecta, a genetic disorder in which osteoblasts produce defective type I collagen, leading to osteopenia, multiple fractures, severe bony deformities and considerably shortened stature. (nih.gov)

Interestingly, we also identified a subpopulation of bone unipotent progenitor expressing TM4SF1+CD44+CD73+CD45-CD31-CD235a- which had osteogenic potentials, but could not reconstitute HME. (bjmu.edu.cn)

Hematopoietic stem cell transplantation has become a major treatment option for patients with hematopoietic malignancies and immune deficiencies. (cdc.gov)

After lethal irradiation, the five animals were given 15-27 X 10(6) autologous marrow cells (3.2-4.4 X 10(6) cells/kg) containing 65-91% CD34+ cells. (nih.gov)

3 Autologous stem cell transplantation almost exclusively uses peripheral blood, while for allogeneic SCT, T-lymphocytes in the peripheral blood could pose some danger. (bdbiosciences.com)
Hematopoietic stem cells have the unique capacity to continuously self-renew and maintain a pool of stem cells for the lifelong production of blood cells. (thenoveldifference.com)
Hematopoietic stem cells are pivotal in maintaining the body's blood cell supply, and their versatility makes them invaluable in both medical treatments and scientific research related to blood disorders and regenerative medicine. (thenoveldifference.com)
For example, hematopoietic progenitor cells are found in the bone marrow, as this is where blood cell production occurs. (thenoveldifference.com)
European Group for Blood and Marrow Transplantation Severe Aplastic Anaemia Working Party. (springermedizin.at)
Specifically, methods are disclosed for mobilizing hematopoietic stem cells into a subject's peripheral blood. (justia.com)
In particular, embodiments of the method involve inhibition of both Rac1 and Rac2 GTPases to increase the numbers of hematopoietic stem cells into a subject's peripheral blood of a subject. (justia.com)
Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
The various mature blood cell types are all ultimately derived from a single class of progenitor cell known as hematopoietic stem cells. (justia.com)
These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
Transplanted tissue may be cells (e.g., hematopoietic stem cells Hematopoietic stem cells Progenitor cells from which all blood cells derived. (lecturio.com)
They are found primarily in the bone marrow and also in small numbers in the peripheral blood. (lecturio.com)
In PV, bone marrow panmyelosis is associated with increased numbers of red blood cells and, in some patients, neutrophilia and/or thrombocytosis. (oncohemakey.com)
FIGURE 2-1 Formation of the multiple peripheral blood cells from multipotent hematopoietic stem cells. (nationalacademies.org)
Later, they were administered high-dose etoposide, 1,500 mg/[m.sup.2], followed by granulocyte colony-stimulating factor for collection of peripheral blood stem cells. (the-medical-dictionary.com)
that is, 'stem cells' just before they are differentiated into blood cells, for processing in the laboratory. (mdanderson.es)
1957). This pioneering work laid the foundation for the exploration of hematopoietic stem cells and their role in treating diseases of the blood and immune system. (revistadehematologia.org.mx)
1989). Hematopoietic stem cells could be harvested easier from peripheral blood after mobilization with G-CSF (Sheridan et al. (revistadehematologia.org.mx)
In December 1994, the International Society for Cellular Therapy (ISCT) and the American Society for Blood and Marrow Transplantation (ASBMT) merged their Standards into a single document covering all aspects of hematopoietic cell therapy (collection, processing, and transplantation). (foxchase.org)
JACIE was founded by the European Group for Blood and Marrow Transplantation (EBMT) and the International Society for Cellular Therapy (ISCT), the two leading scientific organizations involved with cellular transplantation in Europe. (foxchase.org)
During the next year of funding, we will continue to study the hEMP and other blood progenitors with the ultimate goal of learning how to improve production of the lymphoid immune system from hESC. (ca.gov)
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2012 Jan 18 (1): 100-5. (cdc.gov)
Three days after donation of peripheral blood stem cells to a recipient with acute myeloblastic leukemia, dengue virus was detected in the donor, who had recently traveled to Sri Lanka. (cdc.gov)
Here, we report transmission of DENV to a peripheral blood stem cell recipient by a donor who had recently traveled to an area to which the virus is endemic. (cdc.gov)
Examination of peripheral blood smear and bone marrow is diagnostic. (msdmanuals.com)

These CD4+ CD25+ T regulatory cells can be isolated from mesenchymal stem cells and CD34+ hematopoietic stem cells. (hoajonline.com)
Mesenchymal stem cells and immunomodulation: toward new immunosuppressive strategies for the treatment of autoimmune diseases? (staminafoundation.org)

Dr. George Mathé explored the transplantation of bone marrow cells from healthy donors to treat patients accidentally irradiated at high dose, expanding the possibilities of this life-saving technique (Mathé et al. (revistadehematologia.org.mx)
Allogeneic bone marrow transplantation, as we understood it, allows saving lives, but cannot take place without donors. (longdom.org)
If the geno-identical transplantation is impossible, we will direct ourselves towards the national and international register donors of bone marrow. (longdom.org)
Organ Transplantation.2 These Guiding Principles - whose emphases include voluntary donation, noncommercialization, genetic relation of recipients to donors and a preference for cadavers over living donors as sources - have considerably influenced professional codes, national, state and provincial legislation, and the policies of intergovernmental organizations. (who.int)

To qualify as stem cells, self-renewal has to be shown as well [ 10 ]. (hindawi.com)
They produce a defined number of specialized cells and do not have the same capacity for self-renewal as stem cells. (thenoveldifference.com)
True stem cells are both pluripotent-that is they can give rise to all cell types-and capable of self-renewal. (justia.com)
Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)

and single cell RNA-seq technology helped us to gain a global recognition of the heterogeneous populations in the murine bone marrow (BM), however, the cell census of human fetal BM remains underestimated owing to material limitations. (bjmu.edu.cn)

The robustness and efficacy of the retrodifferentiation process in generating unprecedented quantities of stem cells belonging to the three germ layers will enable organ and tissue reconstruction ex vivo, using bio-printing and various scaffold materials. (eurekaselect.com)

The pluripotent stem cells (eg. (hoajonline.com)

Both solid organs and bone marrow Bone marrow The soft tissue filling the cavities of bones. (lecturio.com)
Transplantation of human organs and tissues1 saves many lives and restores essential functions in circumstances when no medical alternative of comparable effectiveness exists. (who.int)
The transplantation of solid organs, such as kidney, liver, heart or lung, is increasingly a regular component of health care in all countries, and is no longer a feature of health care in high-income countries alone. (who.int)
The persistent and widening gap between patients' need for organs and the number available for transplantation has become a major concern to many Member States. (who.int)

Long-term hematopoietic stem cell damage in a murine model of the hematopoietic syndrome of the acute radiation syndrome. (iu.edu)
Establishing a murine model of the hematopoietic syndrome of the acute radiation syndrome. (iu.edu)

Atta-ur-Rahman, Shazia Anjum , " Frontiers in Stem Cell and Regenerative Medicine Research ", Bentham Science Publishers (2017). (eurekaselect.com)
The hematopoietic system is the most regenerative mammalian tissue. (digs-bb.de)
Conclusion: stem cells from dental origin are an interesting alternative for research and application in regenerative therapies in Dentistry. (bvsalud.org)

Transplantation 1997;64:945-947. (postgradualninefrologie.cz)

Anatomy36

Organisms15

Diseases47

Chemicals and Drugs45

Analytical, Diagnostic and Therapeutic Techniques and Equipment68

Psychiatry and Psychology1

Phenomena and Processes48

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science5

Named Groups2

Health Care11

Hematopoiesis11

Transplant14

Chemotherapy15

HSCs22

Differentiation14

Proliferation4

Harvested hematopoietic stem and progenitor cells1

Tissues15

Mobilizing hematopoietic progenitor cells1

Graft versus3

Reconstitution6

Found in bone marrow1

HSCT8

Committed progenitor cells3

Differentiate6

Mobilization of Hematopoietic Stem1

Engraftment5

Cells from the bone marrow1

CD3410

Mice7

Including tissue1

Handling of hematopoietic stem1

Stromal4

Primitive4

Adult6

Regeneration11

Endothelial3

Allogeneic haematopoietic1

Assays2

Lineages3

Early hematopoietic1

Clinical11

Peripheral-blood stem-cell transplant1

Transplants6

MSCs9

Myeloid3

Cord5

Aplastic anemia3

Tumors3

Children with osteogenesis imperfecta1

Reconstitute1

Malignancies1

Autologous marrow1

Blood26

Mesenchymal stem2

Donors4

Self-renewal4

Populations1

Vivo1

Pluripotent stem1

Organs4

Murine2

Regenerative3

19971