Hematopoietic progenitor cells stem cell lineages. Medical search. Frequent questions

Notch activation in human CD34+ hematopoietic stem/progenitor cells (HSPCs) by treatment with Delta1 ligand has enabled clinically relevant ex vivo expansion of short-term HSPCs. (confex.com)
Early hematopoiesis is a continuous process in which hematopoietic stem and progenitor cells (HSPCs) gradually differentiate toward specific lineages. (cun.es)
In this study, we used single cell RNA sequencing (scRNAseq) to characterize an enriched population of human hematopoietic stem and progenitor cells (HSPCs) obtained from young and elderly healthy individuals. (cun.es)
Endothelial cells (ECs) line blood vessels and can serve as specialized vascular niches for hematopoietic stem and progenitor cells (HSPCs), a special environment where HSPCs reside and self-renew. (go.jp)
HSPCs are multipotent cells that can self-renew and differentiate to give rise to all blood cell lineages. (go.jp)
When these islet1 -lineage ECs were specifically ablated, most of the HSPCs were lost from the caudal hematopoietic tissue (CHT), a transient vascular niche for HSPCs in zebrafish (Figure 1B). (go.jp)
islet1 + -derived ECs (green) are mainly located in the caudal hematopoietic tissue (CHT), a transient vascular niche for HSPCs. (go.jp)
Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
During vertebrate embryogenesis , fetal hematopoietic stem and progenitor cells (HSPCs) exhibit expansion and differentiation properties in a supportive hematopoietic niche. (bvsalud.org)
To profile the developmental landscape of fetal HSPCs and their local niche, here, using single- cell RNA -sequencing, we deciphered a dynamic atlas covering 28,777 cells and 9 major cell types (23 clusters) of zebrafish caudal hematopoietic tissue (CHT). (bvsalud.org)
We characterized four heterogeneous HSPCs with distinct lineage priming and metabolic gene signatures. (bvsalud.org)
Hematopoietic stem and progenitor cells (HSPCs) are crucial for the maintenance of homeostasis of all blood lineages in human. (stemcell-lab-mk1dresden.de)
Moreover, HSPCs are of Major relevance for the establishment of successful hematopoietic stem cell transplantation (HSCT) strategies. (stemcell-lab-mk1dresden.de)

For pediatric patients with refractory cytopenia, certain cytogenetic abnormalities, or malignant transformation, hematopoietic stem cell transplantation (HSCT) from a matched related or unrelated donor early in the course of the disease is the treatment of choice. (medscape.com)
However, sustained engraftment of the expanded cells was not observed after transplantation, suggesting ineffective expansion of hematopoietic stem cells with long-term repopulating activity (LTR-HSCs). (confex.com)
At optimal coating densities of Delta1, the frequency of LTR-HSCs measured by limiting dilution analysis 16 weeks after transplantation into NSG mice was 4.9- and 4.2-fold higher in hypoxic cultures (1 in 1,586 CD34+ cells) compared with uncultured cells (1 in 7,706) and the normoxia group (1 in 5,090), respectively. (confex.com)
Conversely, we observed no difference in expression of the homing CXCR4 receptor between cells cultured under normoxic and hypoxic conditions, indicating that hypoxia increased the absolute numbers of LTR-HSCs but not their homing potential after transplantation. (confex.com)
I have expertise in novel therapies for acute myeloid leukemia, stem cell mobilization and homing, as well as stem cell transplantation. (rochester.edu)
HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
Hematopoietic stem cells are widely used in medical treatments, particularly in bone marrow transplantation for individuals with hematological disorders like leukemia or aplastic anemia. (thenoveldifference.com)
MDS is rare in childhood and may have a rapidly progressive course with an extremely poor prognosis without hematopoietic stem cell transplantation (HSCT). (medscape.com)
The specific medications administered depend on the choice of therapy and whether it is supportive care only, immunosuppressive therapy, or hematopoietic cell transplantation. (medscape.com)
Myelosuppressive drugs (eg, hydroxyurea), stem cell transplantation, and interferon alfa are also sometimes used. (msdmanuals.com)

HSCs are self-renewing cells: when they differentiate, at least some of their daughter cells remain as HSCs so the pool of stem cells is not depleted. (wikipedia.org)
C) We have identified that islet1 + endothelial progenitors originate from endoderm and finally differentiate into posterior venous ECs in a process dependent on Bmp-Smad signaling, requiring Npas4l-Etsrp function. (go.jp)
Stem cells rely on extracellular signals produced by the niche, which dictate their ability to self-renew, expand and differentiate. (karger.com)
Neural stem cells can also give rise to neural progenitor cells, which proliferate rapidly during their short lives and then 'differentiate' into neurons or glia. (elifesciences.org)
Since neurosphere-forming cells can self-renew and differentiate into neurons and glia, the ability of cells to form neurospheres has generally been taken as evidence that they are stem cells. (elifesciences.org)
This suggested that EPCs are able to differentiate into endothelial cells and that such cells are incorporated into sites of active angiogenesis including ischemia, tumor angiogenesis, and metastasis in adult organisms [ 16 , 17 ]. (hindawi.com)
They differentiate into very specific cells. (thenoveldifference.com)
HSCs have the remarkable ability to differentiate into a wide range of specialized blood cells. (thenoveldifference.com)
Hematopoietic stem cells give rise to progenitor cells, which, in turn, differentiate into specific blood cell lineages. (thenoveldifference.com)
During haematopoiesis, haematopoietic stem cells differentiate into restricted potential progenitors before maturing into the many lineages required for oxygen transport, wound healing and immune response. (edu.au)
There is growing evidence that resident progenitor cell populations exist in murine lung tissues and differentiate into a mesenchymal cell lineage [1, 2]. (keio.ac.jp)
Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)

page needed] Haematopoietic stem cells (HSCs) reside in the medulla of the bone (bone marrow) and have the unique ability to give rise to all of the different mature blood cell types and tissues. (wikipedia.org)
The other daughters of HSCs (myeloid and lymphoid progenitor cells) can follow any of the other differentiation pathways that lead to the production of one or more specific types of blood cell, but cannot renew themselves. (wikipedia.org)
To corroborate these findings molecularly, we performed transcriptomic analyses and found significant upregulation of a distinct HSC gene expression signature in cells cultured with Delta1 in hypoxia (Fig. A). Collectively, these data show that hypoxia supports a superior ex vivo expansion of human HSCs with LTR activity compared with normoxia at optimized densities of Delta1. (confex.com)
To clarify how hypoxia improved Notch-mediated expansion of LTR-HSCs, we performed scRNA-seq of CD34+ cells treated with Delta1 under normoxic or hypoxic conditions. (confex.com)
Hypoxia lessened the cellular stress response in both progenitors and HSCs, but the mitigation was more apparent in the HSC population (Fig. C, grey plots), and decreased apoptosis was observed only within the HSC-enriched cluster 5 (Fig. D). These findings are consistent with several reports indicating that HSCs are more vulnerable to strong ER stress than downstream progenitors due to their lower protein folding capacity. (confex.com)
In textbook theory, HSCs branch early on into two progenitor cell types, one of which, known as the common lymphoid progenitor (CLP), later differentiates into T and B cells. (riken.jp)
In experiments with mouse HSCs cultured on immobilized delta-like 4 proteins, the researchers found that progenitors developing toward T cells were arrested in the absence of so-called feeder cells, which support survival and growth. (riken.jp)
HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
Using inducible deletion of autophagy specifically in adult hematopoietic stem cells (HSCs) and in mice chimeric for autophagy-deficient and normal HSCs, we demonstrate that the stem cell loss is cell-intrinsic. (ox.ac.uk)
Mechanistically, autophagy-deficient HSCs showed higher expression of several amino acid transporters (AAT) when compared to autophagy-competent cells, resulting in increased amino acid (AA) uptake. (ox.ac.uk)
This was followed by sustained MTOR (mechanistic target of rapamycin) activation, with enlarged cell size, glucose uptake and translation, which is detrimental to the quiescent HSCs. (ox.ac.uk)
Hematopoietic stem cells (HSCs) are a type of multipotent stem cell found primarily in the bone marrow and, to a lesser extent, in peripheral blood. (thenoveldifference.com)
Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)
Hematopoietic stem cells (HSCs) and downstream lineage-biased multipotent progenitors (MPP) tailor blood production. (thekanglab.org)
We study cell fate decision and lineage specification mechanisms in HSCs and MPPs to modulate lineage output in disease and aging context for therapeutic purposes. (thekanglab.org)
Signaling pathways functioning in priming HSCs towards lymphoid or erythroid lineage are not well understood nor the interplays between different signaling pathways in HSCs. (thekanglab.org)
The presence of Meg-biased HSCs and Meg-biased MPP2 generated from HSCs suggests that MPP2 would need to shut down the Meg program to be able to produce erythroid cells. (thekanglab.org)
Hematopoietic Stem Cells (HSCs) are the most thoroughly characterized stem cell population in the body and their study has resulted in well established methods for their isolation, purification and reliable assays of HSC function. (bu.edu)
Vertebrate Hematopoietic Stem Cells (HSCs) are responsible for lifelong maintenance of blood cells. (stemcells-live.fr)
Maintaining HSCs depends on signals provided by a complex micro-environment, called the « niche », which is made of vascular and osteoblastic cells. (stemcells-live.fr)

Wutz, A. Haploid mouse embryonic stem cells: rapid genetic screening and germline transmission. (nature.com)
Leeb, M. & Wutz, A. Derivation of haploid embryonic stem cells from mouse embryos. (nature.com)
Forward and reverse genetics through derivation of haploid mouse embryonic stem cells. (nature.com)
Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells. (nature.com)
Androgenetic haploid embryonic stem cells produce live transgenic mice. (nature.com)
Genetic modification and screening in rat using haploid embryonic stem cells. (nature.com)
Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes. (nature.com)
Derivation and differentiation of haploid human embryonic stem cells. (nature.com)
Inducible Gata1 suppression expands megakaryocyte-erythroid progenitors from embryonic stem cells. (uthsc.edu)
Embryonic Stem Cells (ESC) are pluripotent undifferentiated cells capable of giving rise to cells from all three germ layers. (bu.edu)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)

Heterogeneity within hematopoietic progenitor populations has considerably limited characterization and molecular understanding of lineage commitment in both health and disease. (europa.eu)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
Culture of sorted bone marrow populations on stromal cells secreting interleukin-7 or in fetal thymic organ cultures showed that B and T lymphoid progenitors were also present in the lacZhigh and lacZint fractions. (ozgene.com)
The Haemopedia RNA-seq data set covers a wide range of lineages and progenitors, with 57 mouse blood cell types (flow sorted populations from healthy mice) and 12 human blood cell types. (edu.au)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)

Genetic abnormalities associated with MDS block differentiation of hematopoietic stem and progenitor cells. (medscape.com)
A research team in Japan has linked a developmental checkpoint in the differentiation of hematopoietic progenitors into specific T cell lineages to the activity of a single gene encoding a transcription factor. (riken.jp)

Consequently, investigation of molecular heterogeneity in the differentiation potential of HSPC is vital to identify novel regulators that affect generation of specific cell types, especially RBC. (lu.se)
However, little is known about EC heterogeneity in hematopoietic tissues. (go.jp)
Therefore, our findings are keys to understanding how a lineage-dependent cue regulates EC heterogeneity in future studies. (go.jp)
One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
However, we are still lacking a precise resolution of lineage differentiation trajectories and cellular heterogeneity in the MPP compartment. (thekanglab.org)

When bone marrow develops, it eventually assumes the task of forming most of the blood cells for the entire organism. (wikipedia.org)
During fetal development, since bones and thus the bone marrow develop later, the liver functions as the main haematopoietic organ. (wikipedia.org)
This event explains the presence of multiple derangements observed in the bone marrow that involve several cell lineages. (medscape.com)
As the affected cell lines continue to divide and to provide the marrow with dysplastic cells, bone marrow dysfunction becomes apparent. (medscape.com)
It is arise from hematopoietic stem and progenitor cells in the bone marrow. (medgadget.com)
Among bone marrow hematopoietic stem cells, 70% exhibited detectable telomerase activity. (nih.gov)
NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
Monocytes (MC) are bone marrow (BM) derived mononuclear phagocytes that play an important role in innate immune response and are the major immune cell population in chronic tissue inflammatory ( 1 , 2 ). (frontiersin.org)
Her research group is particularly interested in developing biomaterials and cell-based therapeutics to improve regeneration of various musculoskeletal tissues including bone, cartilage, tendon, blood vessels etc. (stanford.edu)
Myeloid cells are a heterogeneous population of bone marrow-derived cells that play a critical role during growth and metastasis of malignant tumors. (hindawi.com)
These cells were therefore described as bone marrow derived endothelial progenitor cells (EPCs). (hindawi.com)
Haematopoietic stem cells are created during embryo development and they follow a dynamic choreography of anatomical localisation, migrating from the dorsal aorta where they emerge , to the fetal liver and then to the fetal bone marrow. (ox.ac.uk)
It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. (biolegend.com)
Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells. (biolegend.com)
The bone marrow serves as the primary residence for hematopoietic stem cells. (thenoveldifference.com)
For example, hematopoietic progenitor cells are found in the bone marrow, as this is where blood cell production occurs. (thenoveldifference.com)
C57 BL/6 bone marrow cells stained with CD45R/B220 APC+ CD11b APC and CD135 (clone A2F10) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom). (biolegend.com)
Bone marrow cells from heterozygous SCLlacZ/w mice were sorted into fractions expressing high, intermediate and low levels of beta-galactosidase (designated lacZhigh, lacZint, and lacZneg). (ozgene.com)
Myelodysplastic syndrome (MDS) in childhood encompasses a diverse group of bone marrow disorders that share a common clonal defect of stem cells and that result in ineffective hematopoiesis with dysplastic changes in the marrow. (medscape.com)
Size control of the Drosophila hematopoietic niche by bone morphogenetic protein signaling reveals parallels with mammals. (stemcells-live.fr)
Stem cell antigen-1 (Sca-1) is a cell surface glycoprotein, initially found in murine bone marrow-derived stem cell subtypes, such as hematopoietic stem cells. (keio.ac.jp)
Low-power view of hematoxylin-eosin-stained bone marrow showing hypocellularity, with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)
The clinical presentation of patients with aplastic anemia includes signs and symptoms related to the decrease in bone marrow production of hematopoietic cells. (medscape.com)
The bcr-abl oncoprotein has uncontrolled tyrosine kinase activity, which deregulates cellular proliferation, decreases adherence of leukemia cells to the bone marrow stroma, and protects leukemic cells from normal programmed cell death (apoptosis). (msdmanuals.com)
CML ensues when an abnormal pluripotent hematopoietic progenitor cell initiates excessive production of all myeloid lineage cells, primarily in the bone marrow but also in extramedullary sites (eg, spleen, liver). (msdmanuals.com)

The lymphoid lineage is composed of T-cells, B-cells and natural killer cells. (wikipedia.org)
A malignancy in the lymphoid lineage that includes white blood cells such as T lymphocytes and B lymphocytes. (medgadget.com)

As a stem cell matures it undergoes changes in gene expression that limit the cell types that it can become and moves it closer to a specific cell type (cellular differentiation). (wikipedia.org)
To assess the relative impact of normoxia and hypoxia on the HSC compartment, we performed gene set enrichment analysis (GSEA) of cells within HSC cluster 5 from each culture condition. (confex.com)
Based on their transcriptional profile, we identified changes in the proportions of progenitor compartments during aging, and differences in their functionality, as evidenced by gene set enrichment analysis. (cun.es)
Trajectory inference revealed that altered gene expression dynamics accompanied cell differentiation, which could explain age-associated changes in hematopoiesis. (cun.es)
Placing the final piece in the puzzle, the researchers discovered that T cell progenitors in the thymus from mice deficient in the gene Bcl11b exhibited the same halted development and self-renewal cycle, while further experiments connected up-regulation of the transcription factor encoded by this gene to IL-7 levels. (riken.jp)
Together, the findings identify Bcl11b as a "master gene" governing the final step toward differentiation into T cells, confirming the proposed model and heralding a paradigm shift in our understanding of blood cell lineages. (riken.jp)
Genetic rearrangements and point mutations affecting the PDGFRA gene are somatic mutations, which are mutations acquired during a person's lifetime that are present only in certain cells. (medlineplus.gov)
The PDGFRA gene provides instructions for making a receptor protein that is found in the cell membrane of certain cell types. (medlineplus.gov)
When the FIP1L1-PDGFRA fusion gene mutation or point mutations in the PDGFRA gene occur in blood cell precursors, the growth of eosinophils (and occasionally other blood cells, such as neutrophils and mast cells) is poorly controlled, leading to PDGFRA -associated chronic eosinophilic leukemia. (medlineplus.gov)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
It s possible that by targeting this gene with drugs, we may be able to convert malignant B-cell lymphomas into much less harmful histiocytomas, tumors composed of relatively inactive macrophages. (innovations-report.com)
Gene expression data from 24 experiments for 8 different cell types of the human myelopoietic lineage were used to generate an integrated myelopoiesis dataset of 9,425 genes, each reliably associated to a unique genomic position and chromosomal coordinate. (biomedcentral.com)
For instance, the existence of tissue-specific gene clusters may be related to the efficient activation of gene expression in a particular cell lineage, by genetic and epigenetic mechanisms, or related to the repression of entire chromosomal regions containing genes expressed in a specific cell type, e.g. during the developmental switches leading to different cell lineages [ 25 ]. (biomedcentral.com)
To study the functional properties of hematopoietic cells expressing SCL, we have generated mutant mice (SCLlacZ/w) in which the Escherichia coli lacZ reporter gene has been "knocked in" to the SCL locus, thereby linking beta-galactosidase expression to transcription from the SCL promoter. (ozgene.com)
We have updated Haemopedia, a database of gene-expression profiles from a broad spectrum of haematopoietic cells, to include RNA-seq gene-expression data from both mice and humans. (edu.au)
We have added the ability to compare gene expression across data sets and species by curating data sets with shared lineage designations or to view expression gene vs gene, with all plots available for download by the user. (edu.au)
We are combining novel engineered stromal cells with gene editing to explore a way to direct the differentiation of iPSC into hematopoietic progenitors and into a T cell/T reg lineage. (bu.edu)
Continue deciphering the communication between the PSC and hematopoietic progenitors in the LG, and identifying the relevant gene networks. (stemcells-live.fr)
Mutations in the GATA2 gene were found to be the common genetic cause of four syndromes: Emberger syndrome, 1 mycobacterial infections (MonoMAC) syndrome, 2 dendritic cell/monocytopenia/natural killer (NK)-cell/B-cell lymphoid deficiency (DCML) 3 and familial MDS/AML. (healthbooktimes.org)

Hematopoietic stem/progenitor cells (HSPC) are known to be functionally diverse in terms of their self-renewal potential and lineage output. (lu.se)
In this study, we observed that the expression of pseudouridine (Ψ) synthase 10 is increased in aged hematopoietic stem and progenitor cells (HSPC) and enforced protein of Ψ synthase 10 (PUS10) recapitulates the phenotype of aged HSC, which is not achieved by its Ψ synthase activity. (haematologica.org)
In hematopoietic tissues, a group of ECs are highly specialized and constitute the vascular HSPC niche to support HSPC maturation, expansion, and differentiation. (go.jp)
They found that islet1 -expressing cells are the progenitors of the venous ECs that constitute the majority of the HSPC niche (Figure 1A). (go.jp)
The team visualized endothelial differentiation step-by-step from the endoderm to the HSPC niche-constituting ECs via islet1+ endothelial progenitors by live imaging-based lineage tracing. (go.jp)
The present results clearly demonstrate that ECs originating from the endoderm via islet1 + endothelial progenitors play a specialized role in forming a functional vascular HSPC niche (Figure 1C). (go.jp)
Importantly, we identified an endothelial cell -specific G protein -coupled receptor 182, followed by in vivo and in vitro functional validation of its evolutionally conserved role in supporting HSPC expansion in zebrafish and mice . (bvsalud.org)
These findings enhance our understanding of the regulatory mechanism underlying hematopoietic niche for HSPC expansion in vivo and provide insights into improving protocols for HSPC expansion in vitro . (bvsalud.org)

In this study, single-cell RNA-sequencing analyses show that islet1 + -derived ECs express a set of genes that reflects their unique origin even as they become heterogeneous. (go.jp)
Altogether our FACS (fluorescence-activated cell sorter) analyses reveal that the neonatal subventricular zone is far more heterogeneous than previously suspected and our studies provide new insights into the signals and mechanisms that regulate their self-renewal and proliferation. (karger.com)
During limb epimorphic regeneration: committed mesenchymal cells at the stump site dedifferentiate, forming a cluster of heterogeneous population of stem cells, known as the blastema. (eurekaselect.com)
In response to ligation of monomorphic regions of MHC class II antigens with monoclonal antibody CR3/43, human leukocytes retrodifferentiate into a variety of heterogeneous stem cell types belonging to the mesoderm, ectoderm or endoderm lineage, depending on culture media and conditions. (eurekaselect.com)

In summary, we demonstrate that the combination of single cell technologies with computational analysis tools enable the study of a variety of cellular mechanisms involved in complex biological systems such as early hematopoiesis and can be used to dissect perturbed differentiation trajectories associated with perturbations such as aging and malignant transformation. (cun.es)
Melinda is highly proficient in the use of in vitro and in vivo assays for normal and malignant hematopoietic stem and progenitor cells, determination of self-renewal, expansion and lineage specific differentiation capabilities, and the use of induced pluripotent stem cells for the investigation of hematopoietic disorders and leukemic progression and the testing of targeted therapeutics. (edu.au)
Chronic myeloid leukemia (CML) occurs when a pluripotent stem cell undergoes malignant transformation and clonal myeloproliferation, leading to a striking overproduction of mature and immature granulocytes. (msdmanuals.com)
Загальні відомості про лейкоз Leukemia is a malignant condition involving the excess production of immature or abnormal leukocytes, which eventually suppresses the production of normal blood cells and results in symptoms. (msdmanuals.com)

Hematopoiesis is an important model for stem cell differentiation with great medical significance. (europa.eu)
Here, we propose to uncover the hierarchy and regulatory mechanisms controlling hematopoiesis by combining comprehensive single-cell RNA-Seq analyses, modelling approaches, advanced functional assays, single-cell CRISPR screens, knockout models and epigenetic profiling. (europa.eu)
We will pursue the following aims: (i) Generate a refined model of hematopoiesis by comprehensive single-cell RNA-Seq profiling of hematopoietic progenitors, (ii) validate the predicted model by in vivo functional developmental assays and then (iii) test candidate transcription and chromatin factors uncovered by our model for their role in controlling progression towards various lineages using single-cell measurements combined with CRISPR screens. (europa.eu)
Together, our study is expected to generate a revised and high-resolution hematopoietic model and decipher the regulatory networks that control hematopoiesis. (europa.eu)
During regular hematopoiesis, multipotent progenitors (myeloid-T-B progenitors) first lose the potential to generate B cells. (riken.jp)
Hematopoiesis is the process of blood cell formation. (thenoveldifference.com)
The ASH Giuseppe Bigi Memorial Award was established in 2015 to recognize an Italian trainee (undergraduate student, medical student, graduate student, resident physician, or post-doctoral fellow) based at an Italian institution who has the highest-scoring abstract submitted in the field of hematopoiesis and stem cells. (hematology.org)
Recent lineage tracing analyses revealed MPPs to be major functional contributors to steady-state hematopoiesis. (thekanglab.org)
The overall goal is to integrate the different communications between the vascular niche and the LG, and between the PSC and hemocyte progenitors, in order to have a global view of hematopoietic stems cells maintenance of and the control of Drosophila hematopoiesis in normal conditions and upon parasitsism. (stemcells-live.fr)

They are derived from common lymphoid progenitors. (wikipedia.org)

These signaling pathways control many important cellular processes, such as cell growth and division (proliferation) and cell survival. (medlineplus.gov)
As a result, the signaling pathways are constantly turned on (constitutively activated), which increases the proliferation and survival of cells. (medlineplus.gov)
Ly6C high MC presented downregulated co-stimulatory receptors (CD2, GITR, and TIM1) which direct immune cell proliferation, and upregulated co-stimulatory ligands (LIGHT and SEMA4A) which trigger antigen priming and differentiation. (frontiersin.org)
It is now known that new blood vessels originate from preexisting vessels by activation, proliferation and migration of endothelial cells through a process named "angiogenesis" [ 4 ]. (hindawi.com)
Specific growth factors, such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), stimulate the proliferation and migration of naturally quiescent endothelial cells, resulting in the formation of new vessel structures during embryonic development and tumor growth [ 7 ]. (hindawi.com)
While progenitor cells can divide and replicate, their proliferation potential is more restricted compared to stem cells. (thenoveldifference.com)
Gran1 was selectively internalized by macrophages, the major host cell of Mtb , and restricted the proliferation of the pathogen. (uni-ulm.de)

Recently we have developed an experimental and analytical method, termed massively parallel single-cell RNA-Seq (MARS-Seq), for unbiased classification of individual cells from their native context and successfully applied it for characterization of immune and hematopoietic progenitors. (europa.eu)
Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. (nature.com)
Our lab has a major interest in the study of induced Pluripotent Stem cells or iPS cells and the development of tools for their generation and characterization. (bu.edu)

Divergent functions of hematopoietic transcription factors in lineage priming and differentiation during erythro-megakaryopoiesis. (uthsc.edu)

Researchers identify the origin of endothelial cells that constitute the vascular niche for hematopoietic stem and progenitor cells in zebrafish. (go.jp)
Vasculogenesis is the coalescence of new blood vessels from individual endothelial cells or progenitor cells. (hindawi.com)
Upon culture in endothelial growth media, these cells expressed endothelial lineage markers, such as CD31, Tie2, and VEGF receptor 2 (VEGFR2), and incorporated into blood vessels in ischemic tissues. (hindawi.com)
Subsequent studies described a VEGFR2 and AC133 expressing subpopulation of these CD34 positive circulating cells that could form endothelial colonies in vitro [ 14 , 15 ]. (hindawi.com)
The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition. (ox.ac.uk)
Endothelial cell α-globin and its molecular chaperone α-hemoglobin-stabilizing protein regulate arteriolar contractility. (uthsc.edu)

The many distinct sorts of mature blood cells, like red blood cells for carrying oxygen, white blood cells for immune protection and platelets for wound clotting. (medgadget.com)
We assayed telomerase activity in single cells of the hematopoietic and immune systems. (nih.gov)
Neutrophils are the first immune cells that infiltrate the damaged tissue as early as 24 h after injury. (springer.com)
The innate immune system is mainly composed of myeloid lineage cells, such as macrophages, neutrophils, and mast cells [ 2 ]. (hindawi.com)
Importantly, during tumor progression, myeloid cells are implicated in promoting tumor angiogenesis, causing resistance against antiangiogenic therapies in cancer, and suppressing the immune response during cancer [ 3 - 5 ]. (hindawi.com)
These remarkable cells play a crucial role in the production and maintenance of the body's blood and immune cell supply. (thenoveldifference.com)
Our long standing interest in the immune system now focuses in the utilization of iPSC-derived immune cells, including myeloid dendritic cells (mDCs), T cells and others as the basis to study several inflammatory diseases such as lupus erythematosus and the signaling cascade responsible for immune dysregulation. (bu.edu)
Dendritic cells play a central role in the modulation of the immune response and several inflammatory diseases have been shown to have altered mDC function. (bu.edu)
1) Hematopoetic progenitors, microenvironment, and cellular immune responses. (stemcells-live.fr)
The larval LG contains hematopoietic progenitors (pro-hemocytes) for three types of differentiated hemocytes: the plasmatocytes which are monocyte-like cells involved in phagocytosis, the crystal cells which are required for melanisation, an insect-specific immune and wound-healing response, and the lamellocytes which are devoted to encapsulation of foreign bodies too large to be phagocytosed. (stemcells-live.fr)
Ontogeny of the Drosophila larval hematopoietic organ, hemocyte homeostasis and the dedicated cellular immune response to parasitism. (stemcells-live.fr)

No significant alteration of hematopoietic homeostasis and HSC function is observed in young Pus10-/- mice, while aged Pus10-/- mice exhibit mild alteration of hematopoietic homeostasis and HSC function. (haematologica.org)
Control of blood cell homeostasis in Drosophila larvae by the posterior signalling centre. (stemcells-live.fr)
Two Independent Functions of Collier/Early B Cell Factor in the Control of Drosophila Blood Cell Homeostasis. (stemcells-live.fr)
External insults (eg, infections, radiation, drugs) may disrupt stem cell homeostasis in marrow environment, leading to altered growth. (medscape.com)
Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)

In developing embryos, blood formation occurs in aggregates of blood cells in the yolk sac, called blood islands. (wikipedia.org)

03:06 cell functionally in FINA? (yale.edu)
They are functionally distinct from the lymphoid-primed MPP4 and are geared towards the preferential production of myeloid cells with low contribution to the lymphoid lineages. (thekanglab.org)

Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)
Scientists at the University of Pennsylvania School of Veterinary Medicine have found a new wrinkle in the developmental biology dogma that cell differentiation occurs irreversibly as stem cells give rise to increasingly specialized types of offspring cells. (innovations-report.com)
Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
American Journal of Respiratory Cell and Molecular Biology. (lu.se)

Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
This regenerative ability ensures a constant supply of blood cells throughout an individual's lifetime. (thenoveldifference.com)
Hematopoietic stem cells are pivotal in maintaining the body's blood cell supply, and their versatility makes them invaluable in both medical treatments and scientific research related to blood disorders and regenerative medicine. (thenoveldifference.com)
Progenitor cells are being explored for various therapeutic applications, including tissue engineering and regenerative medicine. (thenoveldifference.com)
The intricate molecular mechanisms involved in the regenerative process of the normal intestine and the identity of putative somatic intestinal stem cells have become clear. (mdpi.com)
Microglial phagocytosis of apoptotic cells (efferocytosis) is at the core of the brain regenerative response. (frontiersin.org)
Atta-ur-Rahman, Shazia Anjum , " Frontiers in Stem Cell and Regenerative Medicine Research ", Bentham Science Publishers (2017). (eurekaselect.com)
While being much smaller compartments than MPP4 at steady-state, MPP2 and MPP3 are transiently expanded in regenerative conditions and are essential for rebuilding the myeloid lineage. (thekanglab.org)
We now aim at using iPS cells for disease modeling disease modeling and study their potential for regenerative medicine. (bu.edu)
They may also serve as an unlimited source of therapeutically active cells for regenerative medi- cine. (lu.se)

Aberration occurs in a stem cell that can give rise to multiple lineages. (medscape.com)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)

They also serve as specialized vascular niches that provide instructive signals to tissue-specific stem cells or parenchymal cells for tissue formation and repair. (go.jp)
Dr. Yang has extensive expertise in developing novel biomaterials and stem cell-based therapeutics for musculoskeletal tissue engineering, or engineering 3D in vitro cancer models for drug screening and mechanistic discovery. (stanford.edu)
Unlike some other tissues, it has not been possible to identify or purify neural stem cells directly from the tissue. (elifesciences.org)
Progenitor cells play a crucial role in tissue repair and regeneration. (thenoveldifference.com)
When specific tissue or organ damage occurs, these cells can rapidly generate new cells of the desired type to facilitate the healing process. (thenoveldifference.com)
They are committed to a specific cell lineage but retain the ability to generate a limited range of related cell types, making them essential for tissue repair, regeneration, and potential medical interventions. (thenoveldifference.com)
An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
Microglia are similar, but not identical to other tissue macrophages, and in this review, we will first summarize the differences in the origin, lineage and population maintenance of microglia and macrophages. (frontiersin.org)
But how different are microglia really from other tissue resident macrophages in terms of origin, lineage, and identity? (frontiersin.org)
In the next sections, we will review evidence about the origin, lineage, identity, and population dynamics of microglia compared to other tissue-resident macrophages and highlight commonalities and differences. (frontiersin.org)
During this process, leukocytes lose lineage-associated markers home and undergo homocytic aggregation, upregulate expression of stem cell antigens, and subsequently redifferentiate to give rise original tissue or, transdifferentiate into a different tissue altogether. (eurekaselect.com)
The robustness and efficacy of the retrodifferentiation process in generating unprecedented quantities of stem cells belonging to the three germ layers will enable organ and tissue reconstruction ex vivo, using bio-printing and various scaffold materials. (eurekaselect.com)
Epimorphic regeneration and retrodifferentiation both have the capacity to recreate and reconstruct tissue with precise positional integration of cells in such a way that will enable us to heal without scars and to understand how to maintain tissue integrity and architecture in the face of a hostile environment. (eurekaselect.com)
Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)

Progenitor cells are often located within specific tissues, close to where they will ultimately function. (thenoveldifference.com)
They offer the potential to replace damaged or dysfunctional cells in specific tissues or organs. (thenoveldifference.com)
We modeled the development of embryonic tissues from hPSCs and delineated the ontogeny of hematopoietic cells by mitochondrial variant lineage tracing. (biorxiv.org)
The Notch signaling pathway is an evolutionary conserved signal transduction cascade present in almost all tissues and is required for embryonic and postnatal development, as well as for stem cell maintenance, but it is also implicated in tumorigenesis including pancreatic cancer and leukemia. (uni-ulm.de)
Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)

Cells of the myeloid lineage, which include granulocytes, megakaryocytes, monocytes and macrophages, are derived from common myeloid progenitors, and are involved in such diverse roles as innate immunity and blood clotting. (wikipedia.org)
Splenic macrophages and adhesion molecules may be involved in regulation of extramedullary myeloid cell generation in cardiovascular disease. (wikipedia.org)
Although tumor cells were first thought to drive the cellular events underpinning tumor angiogenesis and growth, considerable evidence has now emerged for the central role of tumor infiltrating myeloid cells such as monocytes, macrophages, and neutrophils in this phenomenon [ 8 - 12 ]. (hindawi.com)
The researchers have shown that certain mouse cells retain an ability to oscillate between very distinct blood cell types - B-cells and macrophages - long after what has been commonly regarded as the point of no return. (innovations-report.com)
We suspect that this phenomenon is not limited to B-cells and macrophages in mice. (innovations-report.com)
Previous research has demonstrated that when B-cells are transplanted from an animal into a petri dish, the cells sometimes change into macrophages. (innovations-report.com)
Moreover, the cells could continue oscillating between B-cells and macrophages indefinitely, as many times as they were relocated. (innovations-report.com)
B-cells arise from a lymphoid progenitor, while macrophages come from a myeloid progenitor. (innovations-report.com)

We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. (nature.com)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
Our data also suggested that expression of SCL was transient and confined to hematopoietic stem and/or progenitor cells, because the differentiated progeny of most lineages (except the erythroid) were beta-galactosidase-negative. (ozgene.com)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)

Aged hematopoietic stem cells (HSC) exhibit compromised reconstitution capacity and differentiation-bias towards myeloid lineage, however, the molecular mechanism behind it remains not fully understood. (haematologica.org)
Blood production skews towards myeloid lineage upon aging and myeloid malignancies with expanded MPP2 and MPP3. (thekanglab.org)

As an alternative, the clone may progressively deteriorate, and the appearance of marrow may return to normal as healthy stem cells repopulate it. (medscape.com)
Effects of hematopoietic stem cell adhesion on marrow stromal cell cytokine p. (rochester.edu)
The theoretical basis for marrow failure includes primary defects in or damage to the stem cell or the marrow microenvironment. (medscape.com)

Despite these efforts, few genes are known to play a role in the processes of stem cell self-renewal and differentiation. (bu.edu)

Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
Molecular Cell 55, 807-817. (ox.ac.uk)
2018. Molecular cell. (biolegend.com)

All cellular blood components are derived from haematopoietic stem cells. (wikipedia.org)
We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application. (bdbiosciences.com)
Autophagy preserves hematopoietic stem cells by restraining MTORC1-mediated cellular anabolism. (ox.ac.uk)
Human myelopoiesis is an exciting biological model for cellular differentiation since it represents a plastic process where multipotent stem cells gradually limit their differentiation potential, generating different precursor cells which finally evolve into distinct terminally differentiated cells. (biomedcentral.com)

Pre-GEPCOT cells could not form neurospheres but expressed the stem cell markers Slc1a3-CreER T , GFAP-CreER T2 , Sox2 CreERT2 , and Gli1 CreERT2 and were long-lived in vivo. (elifesciences.org)

Using previous findings in healthy cells as a reference, we analyzed scRNA-seq data obtained from patients with myelodysplastic syndrome (MDS) and detected specific alterations of the expression dynamics of genes involved in erythroid differentiation in all patients with MDS such as TRIB2. (cun.es)

Ly6C high MC exhibited activated neutrophil degranulation, lysosome, cytokine production/receptor interaction and myeloid cell activation pathways, and Ly6C low MC presented features of lymphocyte immunity pathways in both mice. (frontiersin.org)
Physiologic aging in both humans and mice leads to permanent changes in LT-HSC function, such as myeloid-biased hematopoietic output ( Akunuru and Geiger, 2016 ). (biorxiv.org)

And, malignancy in the myeloid lineage that includes precursor cells to red blood cells, platelets and white blood cells such as granulocytes. (medgadget.com)
Progenitor cells, also known as precursor cells, are a type of cell that falls somewhere between stem cells and fully differentiated, specialized cells in terms of their differentiation potential. (thenoveldifference.com)

The team published their findings in the journal Developmental Cell . (go.jp)
Bcl11b deficiency induces developmental arrest at the myeloid-T progenitor stage, and the arrested myeloid-T progenitors enter a self-renewal cycle. (riken.jp)
Du ring this developmental window the embryonic/ fetal stem cells and progenitors are prone to DNA damage that drives their loss, or may fuel genetic changes such as chromosomal translocations/mutations that can lead to childhood leukaemia and later in adulthood to clonal blood cell production. (ox.ac.uk)
A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish. (bvsalud.org)

Ly6C low MC manifested activated T-cell signaling pathways and potentially can adapt the function of lymphocytes. (frontiersin.org)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
One focus of our lab is differentiating mouse ESC into intestinal epithelial cells in order to understand the complex signaling pathways involved in intestinal commitment from endodermal progenitors and undifferentiated stem cells. (bu.edu)

By accessing primary human target cells we hope to establish a new paradigm in the use of iPSC-derived cells for infectious diseases. (bu.edu)
We aim at generating iPSC containing mutations in IRF5, Myd88, UNC93, TLRs and other relevant genes to test their role in mDC function compared to normal cells. (bu.edu)

The discovery of the Posterior Signaling Center (PSC), a group of cells controlling blood cell differentiation in the larval Drosophila hematopoietic organ, the lymph gland (LG), has made Drosophila a model to investigate the interaction between hematopoietic progenitors and their microenvironment. (stemcells-live.fr)

13 ] isolated mononuclear cells from human peripheral blood that were enriched for expression of the hematopoietic stem cell marker CD34 [ 13 ]. (hindawi.com)

We explored the use of naturally occurring somatic mutations in mitochondrial transcripts detected in single-cell RNA-seq as genetic lineage barcodes in HPSC. (biorxiv.org)

Cell Stem Cell 19 , 569-572 (2016). (nature.com)

erythroid cells after in vitro differentiation, despite having similar frequencies of phenotypic HSC initially. (lu.se)
02:47 and red blood cells in vitro. (yale.edu)
The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)

We further applied mitochondrial variant lineage tracing to spatial transcriptomics and identified the clonal development of both erythro-myeloid progenitors and their niche cells. (biorxiv.org)
Stem cell niche. (bvsalud.org)

MDS is a clonal disorder of myeloid stem cells. (medscape.com)
The somatic mutation occurs initially in a single cell, which continues to grow and divide, producing a group of cells with the same mutation (a clonal population). (medlineplus.gov)
Our study highlights the power of mitochondrial variants as an endogenous marker for clonal tracking in modeling human development from stem cells. (biorxiv.org)

In a healthy adult human, roughly ten billion (1010) to a hundred billion (1011) new blood cells are produced per day, in order to maintain steady state levels in the peripheral circulation. (wikipedia.org)
All blood cells are divided into three lineages. (wikipedia.org)
Red blood cells, also called erythrocytes, are the oxygen-carrying cells. (wikipedia.org)
The number of reticulocytes, immature red blood cells, gives an estimate of the rate of erythropoiesis. (wikipedia.org)
Between 1948 and 1950, the Committee for Clarification of the Nomenclature of Cells and Diseases of the Blood and Blood-forming Organs issued reports on the nomenclature of blood cells. (wikipedia.org)
Hematopoietic stem cells (HSC) give rise to all types of blood lineages, including red blood cells (RBC). (lu.se)
Hematopoietic stem cells (HSC) generate all of the blood cells throughout their life-span. (haematologica.org)
Reported in Science , the discovery lends support to a new model for the development of blood cells lineages. (riken.jp)
While varying widely in function, all blood cells share a common origin in progenitors known as hematopoietic stem cells (HSC). (riken.jp)
characterized by an elevated number of cells called eosinophils in the blood. (medlineplus.gov)
Some people with PDGFRA -associated chronic eosinophilic leukemia have an increased number of other types of white blood cells, such as neutrophils or mast cells. (medlineplus.gov)
Occasionally, people with PDGFRA -associated chronic eosinophilic leukemia develop other blood cell cancers, such as acute myeloid leukemia or B-cell or T-cell acute lymphoblastic leukemia or lymphoblastic lymphoma. (medlineplus.gov)
Mammalian cells require oxygen and nutrients for their survival and are therefore located within 100 to 200 m of blood vessels, the diffusion limit of oxygen. (hindawi.com)
In 1971, Dr. Judah Folkman observed that neovascularization occurs around tumors and proposed that new blood vessel growth is necessary to supply nutrients and oxygen to tumor cells during exponential tumor growth [ 6 ]. (hindawi.com)
02:14 progenitors that make red blood cells. (yale.edu)
These latest findings on the phenomenon sometimes referred to as "lineage promiscuity" appear on the Web site of the journal Blood and will be published in the journal s print edition in March 2003. (innovations-report.com)
Pushing mature cells into other lineages may offer a new way to replace cells involved in blood diseases and neurodegenerative disorders such as Alzheimer s disease. (innovations-report.com)
Until recently, it was believed that the various cell types of the blood are generated from stem cells in a controlled but irreversible fashion," said James Hagman, an immunology researcher at the National Jewish Medical and Research Center in Denver who was not involved in this research. (innovations-report.com)
They are like giants, capable of turning into any blood cell in our body. (thenoveldifference.com)
These include red blood cells (erythrocytes), white blood cells (leukocytes), and platelets (thrombocytes), all of which are vital for various functions in the body. (thenoveldifference.com)
Hematopoietic stem cells have the unique capacity to continuously self-renew and maintain a pool of stem cells for the lifelong production of blood cells. (thenoveldifference.com)
Here, they reside in specialized niches where they remain quiescent until needed to replenish and repair the blood cell population. (thenoveldifference.com)
They have already committed to a particular differentiation pathway, meaning they are destined to become a specific type of cell, such as a red blood cell, white blood cell, or platelet in the case of hematopoietic progenitor cells. (thenoveldifference.com)
For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. (biolegend.com)
Research hematology with an emphasis on understanding the mechanisms of normal blood cell development associated diseases. (uthsc.edu)
His research interests are to better understand the mechanisms of normal red blood cell development and use this information to treat common blood disorders, including sickle cell disease and betathalassemia. (uthsc.edu)
The ease by which various stem cell types can be generated from human peripheral blood has allowed the design of various kits to guarantee the specificity, sterility and efficacy of stem cells production for various clinical and research applications. (eurekaselect.com)
Therefore, our ultimate goal is to modulate this skewed lineage output to rebalance blood production. (thekanglab.org)
We have previously developed a single lentiviral vector expressing a stem cell cassett, named STEMCCA, capable of generating iPS cells from post-natal fibroblasts and peripheral blood with very high efficiency that became the industry standard. (bu.edu)
Haploinsufficiency of GATA2 leads to impaired genesis and function of hematopoietic stem and progenitor cells, resulting in impairment of all subsequent blood cell lineages. (healthbooktimes.org)
Although granulocyte production predominates, the neoplastic clone includes red blood cells, megakaryocytes, monocytes, and even some T cells and B cells. (msdmanuals.com)

Some studies showed Sca-1 expression in the pulmonary vascular endothelium of adult murine lungs [3], while a subset of Sca-1-expressing cells formed vascular-like structures under specific conditions [1]. (keio.ac.jp)

CD117 (c-kit) serves as a receptor for c-Kit ligand (aka, stem cell factor or SCF). (bdbiosciences.com)
Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. (biolegend.com)

Therefore, we established a rapid screening method of whole zebrafish larva using imaging technologies to identify modulators of hematopoietic stem cell activity. (stemcell-lab-mk1dresden.de)

These islet1 -expressing cells surprisingly originate not from the mesoderm, the germ layer classically defined to give rise to ECs, but from the endoderm. (go.jp)
Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)

In a model put forth nearly ten years ago, the researchers proposed a radically different picture in which progenitor cells retain the potential to generate myeloid cells across all branches of development. (riken.jp)
Thomas-Tikhonenko and colleagues found that when the cells are returned to the animal, they generate spherical B-cell lymphomas, indicating that they have changed their identity yet again. (innovations-report.com)
These cells are committed to developing into a specific type of cell but still retain the capacity to proliferate and generate a limited range of related cell types. (thenoveldifference.com)
We have also modified it to make it excisable and have used it to generate mouse and human iPS cells free of exogenous transgenes. (bu.edu)
that is, they have the ability to generate other stem cells and perpetuate themselves. (bvsalud.org)

The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
The hematopoietic retrodifferentiated stem cells have been shown to engraft an animal host in two proofs of principle clinical studies, demonstrating long-term engraftment and safety in acquired aplastic anaemia, while transient amelioration of beta thalassemia major was also observed. (eurekaselect.com)

Macroautophagy/autophagy is a fundamental survival mechanism that allows cells to adapt to metabolic changes by degrading and recycling intracellular components. (ox.ac.uk)
The aim of this project is to better understand how maternal metabolism and DNA damage repair and the DNA damage response shape early haematopoietic stem cell instruction, survival and dysfunction. (ox.ac.uk)
Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells. (biolegend.com)

In line with this, RBPJL is able to fully reconstitute transcriptional repression at Notch target genes in cells lacking RBPJ. (uni-ulm.de)

2020. Cell. (biolegend.com)

However, the following nomenclature seems to be, at present, the most prevalent: Osteoclasts also arise from hemopoietic cells of the monocyte/neutrophil lineage, specifically CFU-GM. (wikipedia.org)
Neurons that arise in the adult nervous system originate from neural stem cells and neural progenitor cells. (elifesciences.org)

Human adult mobilized CD34+ cells were cultured for 21 days under normoxia (21% O 2 ) or hypoxia (2% O 2 ) in vessels coated with optimized concentrations of Delta1. (confex.com)
Our methods and models may provide a platform for future medical advancements including a large-scale European collaborative project to discover a comprehensive human hematopoietic tree. (europa.eu)
Human MC were initially divided into three subsets based on the cell surface expression of CD14 and CD16, and recently classified based on CD40 expression ( 2 - 5 ). (frontiersin.org)
Using our recently established haploid human pluripotent stem cells (hPSCs), we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs. (nature.com)
The analysis of genomic expression during human myelopoiesis using an integrative computational approach allowed discovering important relationships between genomic position, biological function and expression patterns and highlighting chromatin domains, including genes with coordinated expression and lineage-specific functions. (biomedcentral.com)
Before that, Dr. Weiss was the Associate Director for The Human Embryonic Stem Cell Core Facility at The Children's Hospital of Philadelphia. (uthsc.edu)
Human pluripotent stem cells (hPSC) even face risks of DNA-damage-induced toxicity-related cell death. (biorxiv.org)
Granulysin is an antimicrobial peptide (AMP) expressed by human T-lymphocytes and natural killer cells. (uni-ulm.de)
Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
Here we describe how this system can be used for human embry- onic stem cells. (lu.se)

MPP2 preferentially produces megakaryocytes while maintaining other lineage potentials like erythroid lineage in permissive conditions. (thekanglab.org)

Most clusters could be computationally assigned to a defined hematopoietic subpopulation, including progenitor cells (clusters 0 to 4) and a single transcriptionally defined HSC population (cluster 5). (confex.com)

Tumors exhibit significant myeloid cell infiltrates, which are actively recruited to the tumor microenvironment. (hindawi.com)
Finally, we will comment on the mechanisms regulating myeloid cell recruitment to the tumor microenvironment and on the potential of myeloid cells as new targets for cancer therapy. (hindawi.com)

Anatomy58

Organisms14

Diseases10

Chemicals and Drugs88

Analytical, Diagnostic and Therapeutic Techniques and Equipment44

Phenomena and Processes43

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science2

HSPCs17

Transplantation12

Differentiate12

HSCs23

Embryonic stem12

Populations9

Differentiation of hematopoietic2

Heterogeneity5

Bone25

Lymphoid lineage2

Gene20

HSPC8

Heterogeneous4

Malignant4

Hematopoiesis9

Common lymphoid progenitors1

Proliferation7

Characterization3

Functions of hematopoietic1

Endothelial6

Immune11

Homeostasis5

Embryos1

Functionally2

Biology5

Regenerative10

Multiple lineages2

Tissue14

Tissues6

Macrophages8

Progeny4

Towards myeloid lineage2

Marrow3

Self-renewal and differentiation1

Molecular3

Cellular5

Vivo1

Erythroid differentiation1

Mice2

Precursor cells2

Developmental4

Pathways3

IPSC2

Organ1

CD341

Genetic1

20161

Vitro3

Niche2

Clonal3

Blood31

Murine1

Ligand2

Zebrafish1

Give rise2

Generate5

Clinical2

Survival3

Transcriptional1

20201

Arise2

Human10

Megakaryocytes1

Subpopulation1

Myeloid cell2