Hematopoietic niche stromal cells primitive hematopoietic stem cells. Medical search. Frequent questions

Mesenchymal stromal cells (MSCs) from human bone marrow serve as a resource for cell-based therapies in regenerative medicine. (fairdomhub.org)
MSCs exhibited the typical fibroblastoid morphology, with distinct differences in cell size depending on the medium. (fairdomhub.org)
In previous studies human mesenchymal stromal cells (MSCs) maintained the "stemness" of human hematopoietic progenitor cells (HPCs) through direct cell-cell contact in two-dimensional co-culture … systems. (fairdomhub.org)
The most commonly used type of stem cells, mesenchymal stem cells (MSCs), can be easily isolated from bone marrow or synovium and cultured in vitro . (openorthopaedicsjournal.com)
These osteoconductive three dimensional constructs seeded with MSCs are highly porous, biodegradable and biomechanically stable scaffolds which do not evoke an immunogenic host cell response. (openorthopaedicsjournal.com)
Research has shown the importance of growth factors in guiding and modulating the differentiation of MSCs in order to obtain the required cell type. (openorthopaedicsjournal.com)
Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. (issca.us)
Later, Arnold Caplan's group described mesenchymal stem/stromal cells (MSCs) as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies [2-4]. (issca.us)
Pivotal studies by the group of Horwitz in children with osteogenesis imperfecta, an inherited enzyme deficiency of collagen synthesis by mesenchymal cells in bone, opened the field for intravenous use of MSCs. (issca.us)
The tissue source of the MSCs was in most cases not decisive, and cells from various tissue sources were explored. (issca.us)
and signals from the injected cells were found early after administration of the MSCs at the highest frequencies in the lungs, followed by liver and spleen. (issca.us)
Insulin-producing cells (IPCs) differentiated from mesenchymal stem cells (MSCs) hold great promise in the treatment of DM. (biomedcentral.com)
Laminin 411 markedly induced the expression of the genes Foxa2 and Sox17 , markers for pancreatic precursor cells, efficiently induced IPC differentiation from MSCs, and up-regulated insulin expression at both mRNA and protein levels. (biomedcentral.com)
Mesenchymal stem cells (MSCs) have gained interest because of its potential application in regeneration medicine and cytotherapy. (biomedcentral.com)

For example, it has been suggested that homophilic, N-cadherin-mediated adhesion between HSPC and mesenchymal stem cells (MSC) supports long-term maintenance of the primitive HSPC pool4C6. (conferencedequebec.org)

Dexter observed that mesenchymal stromal cells could maintain early HSCs ex vivo, and both Lord and Gong showed that these cells localized to the endosteal margins in long bones. (wikipedia.org)
These more advanced hematopoietic progenitors isolated from the aorta-gonad-mesonephros (AGM) region at embryonic day 9.5 to 10 can give rise to lymphoid lineages and are LT reconstituting definitive HSCs, but since this time point is after the establishment of the circulatory system in the embryo their source was difficult to determine [ 13 ]. (biomedcentral.com)
HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
While this role has been best documented in the developmental migration of the neural crest and mesoderm, here we expand on previously reported preliminary findings that morpholino knock-down of snai2 impairs the generation of hematopoietic stem cells (HSCs) during zebrafish development. (bvsalud.org)
HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche. (thermofisher.com)
The second wave, referred to as definitive hematopoiesis, temporarily occurs in the fetal liver and transitions to the bone marrow and thymus, where multipotent hematopoietic stem cells (HSCs) contribute to the generation of all blood lineages ( 1 - 3 ). (amegroups.org)
These HSCs are primarily derived from endothelial cells within the ventral wall of the dorsal aorta. (amegroups.org)
The definitive HSCs in mammals first migrate to and repopulate in the fetal liver and then home to the bone marrow, while the definitive HSCs in zebrafish migrate first to the caudal hematopoietic tissue and then to their definitive anatomical sites in the kidney and thymus ( 5 - 8 ). (amegroups.org)

The CLP then goes on to differentiate into more committed lymphoid precursor cells. (wikipedia.org)
The CMP can then further differentiate into the megakaryocyte-erythroid progenitor cell (MEP), which goes on to make RBCs and platelets, or the granulocyte/macrophage progenitor (GMP), which gives rise to the granulocytes of the innate immune response. (wikipedia.org)
There are 2 types of hematopoiesis that occur in humans: Primitive hematopoiesis - blood stem cells differentiate into only a few specialized blood lineages (typically isolated to early fetal development). (wikipedia.org)
These cancer stem cells have the capacity to both divide and expand the cancer stem cell pool and to differentiate into the heterogeneous nontumorigenic cancer cell types that in most cases appear to constitute the bulk of the cancer cells within the tumor. (aacrjournals.org)
and the RBI cells are derived from the lateral mesoderm of the zebrafish head and differentiate into macrophages ( 4 - 8 ). (amegroups.org)
Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)
cells either divide asymmetrically to preserve stem cell progenitors, partition into sister cells, differentiate along fate pathways, or undergo oncogenesis following the formation of normal tissues. (bioseek.eu)

Effects of hematopoietic stem cell adhesion on marrow stromal cell cytokine p. (rochester.edu)

CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
Here, we profile the transcriptome of the earliest detectable endothelial cells (ECs) during zebrafish embryogenesis to demonstrate that tissue-specific EC programs initiate much earlier than previously appreciated, by the end of gastrulation. (bvsalud.org)
If the cancer stem cell model is correct and if such cells retain the hallmarks of some tissue stem cells in being rare and entering the cell cycle infrequently, they could constitute a population that is intrinsically resistant to current therapies designed to kill cycling cells. (aacrjournals.org)
The aim of this study was to comparatively assess the efficiency of xeno-free human platelet lysate (HPL)-based cell expansion with two commercially available media-StemPro MSC SFM CTS (for human ex vivo tissue and cell culture processing applications) and MSCGM (non-GMP-compliant, for research only)-in an academic setting as the first optimization step toward GMP-compliant manufacturing. (fairdomhub.org)
Stem cell therapy is an exciting and upcoming branch of tissue engineering with application in the field of orthopaedics. (openorthopaedicsjournal.com)
Initial studies indicate an immense potential for cell based strategies to enhance current orthopaedic approaches in skeletal tissue reconstruction. (openorthopaedicsjournal.com)
Nevertheless, stem cell based tissue engineering in orthopaedics shows a promising future. (openorthopaedicsjournal.com)
Amidst this, tissue engineering using stem cells has emerged as a hot topic of research and is a promising alternative to the traditional techniques of bone grafting. (openorthopaedicsjournal.com)
A knowledge from the systems that regulate this differentiation potential can lead to book remedies for disorders of bone tissue aswell as options for conserving the integrity of endosteal hematopoietic niches. (morainetownshipdems.org)
Intro Bone and bone tissue marrow (BM) are anatomically contiguous and harbor cell types that are functionally interrelated.1 Conceivably Genkwanin a stem cell could bring about both hematopoietic and osteopoietic progeny beneath the control of a particular genetic system or particular environmental cues. (morainetownshipdems.org)
Cellulose nanofibers (CNF) hydrogel has great potential as a cell-encapsulation delivery carrier for sustained release of paracrine factors and for tissue regeneration, with unique versatility for injection, scaffolding, and 3D bioprinting. (longdom.com)
Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)
Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)

Following verification of a stem-like signature (upregulated expression of stemness markers, resistance to chemotherapy, increased spheroid formation, and tumorigenesis capabilities despite inoculation of a small number of cells), we analyzed the interaction of these cells with macrophages via direct and indirect coculture assays. (springer.com)
In colony-forming unit assays, the hematopoietic stem cell plasticity remained similar after 14 days of bioreactor co-culture, whereas monolayer co-cultures showed increasing signs of HPC differentiation and loss of stemness. (fairdomhub.org)
These results together with proof that bipotential cell satisfies strict requirements for stemness recommend a book system for hematopoietic-osteopoietic maintenance that may be harnessed for medical interventions. (morainetownshipdems.org)

Efficient mobilization of HSPC is a prerequisite for the successful stem cell collection and consecutive transplantation. (conferencedequebec.org)
It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
I have expertise in novel therapies for acute myeloid leukemia, stem cell mobilization and homing, as well as stem cell transplantation. (rochester.edu)
Identifying a transplantable osteoprogenitor or simply a putative dual hematopoietic-osteopoietic progenitor could possibly be key to your knowledge of the biology of marrow transplantation as well as the hematopoietic stem cell (HSC) market. (morainetownshipdems.org)
To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
In these studies, however, transplanted cells were followed, if at all, at the site of transplantation, and biodistribution was not an issue. (issca.us)
This concept started from the observation that bone marrow transplantation can provide stromal cells able to synthesize intact collagen type I, replacing deficient patient cell function and ameliorating disease symptoms [5]. (issca.us)

As the embryo requires rapid oxygenation due to its high mitotic activity, these islands are the main source of red blood cell (RBC) production via fusing endothelial cells (ECs) with the developing embryonic circulation. (wikipedia.org)
Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite-derived endothelial cells (SDECs) that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. (bvsalud.org)

The future direction of the study of hematopoietic stem cells will probably make use of pluripotent stem cells to yield specific immune cell lineages and eventual clinical applications. (biomedcentral.com)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)

Many pathological states can arise from disturbances in this niche environment, highlighting its importance in maintaining hematopoiesis. (wikipedia.org)
Hematopoiesis involves a series of differentiation steps from one progenitor cell to a more committed cell type, forming the recognizable tree seen in the adjacent diagram. (wikipedia.org)
The pioneering work of Till and McCulloch in 1961 experimentally confirmed the development of blood cells from a single precursor hematopoietic stem cell (HSC), creating the framework for the field of hematopoiesis to be studied over the following decades. (wikipedia.org)
Primitive hematopoiesis is first found in the blood islands (Pander's islands) of the yolk sac at E7.5 (embryonic day 7.5) in mice and 30dpc (30 days post-conception) in humans. (wikipedia.org)
Mouse hematopoiesis was thought to develop initially in the yolk sac, where primitive hematopoietic development occurs [ 4 ]. (biomedcentral.com)
Their results indicated that yolk sac progenitors before the onset of circulation did not have LT reconstitution potential while progenitors isolated from the AGM did, which indicates that primitive and definitive hematopoiesis may occur at distinct anatomical locations [ 10 ]. (biomedcentral.com)
A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. (thermofisher.com)
Secondary Genkwanin BMT studies demonstrated that a single marrow cell able to contribute to hematopoietic reconstitution in primary recipients also drives robust osteopoiesis and LT hematopoiesis in secondary recipients. (morainetownshipdems.org)
Using supplementary BMT assays we display here a solitary marrow cell in a position to donate to hematopoietic reconstitution in major recipients drives both osteopoiesis and long-term (LT) hematopoiesis in supplementary recipients. (morainetownshipdems.org)
The first wave, defined as primitive hematopoiesis, takes place in the extraembryonic yolk sac and generates transitory hematopoietic cell populations consisting of primitive erythrocytes. (amegroups.org)
In zebrafish, primitive hematopoiesis occurs at early somite stages in the intermediate cell mass (ICM) and the rostral blood island (RBI). (amegroups.org)
Despite these apparent differences in definitive hematopoiesis between fish and mammals, the definitive HSC niche functions to actively recruit stem cells and maintain their plasticity throughout adulthood ( 2 , 4 , 5 ). (amegroups.org)
There is growing evidence that during primitive hematopoiesis in the developing embryo that macrophages generated in the yolk sac are involved in the regulation of primitive HSC trafficking-a mechanism suggested to involve in the secretion of inflammatory factors, extracellular matrix (ECM) components, and metalloproteinases ( 3 , 9 , 10 ). (amegroups.org)
Figure 1 VCAM-1 + macrophages guide hematopoietic stem/progenitor cell to sites of definitive hematopoiesis. (amegroups.org)
Primitive hematopoiesis occurs at early somite stages in the ICM and the RBI. (amegroups.org)
Subset of macrophages expressing ICAM-1 migrate to the sites of definitive hematopoiesis in AGM and CHT to recruit hematopoietic stem cells and initiate neonatal hematopoiesis and guide them further to sites of adult and definitive hematopoiesis in kidneys and thymus. (amegroups.org)
In the 1970s, Friedenstein and colleagues [1] first reported that locally applied culture-expanded populations of bone marrow stroma-derived fibroblastic cells remained at their injection sites under the kidney capsule, where an ectopic hematopoiesis was initiated. (issca.us)

The participants critically discussed the need for a precise definition of cancer stem cells, the requirement for new markers and more rapid and tractable in vitro and in vivo assays, and the need to develop drug screening strategies to selectively target cancer stem cells to generate therapeutics for this subpopulation of cells that could be resistant to classic treatments while possessing potent tumor-forming capacity. (aacrjournals.org)

Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids. (stembook.org)

Early in murine embryonic development, hematopoietic progenitors can be found in the para-aortic splanchnopleura region on embryonic day 7 (as depicted in Figure 1 A), but these progenitors are not LT reconstituting although they do possess lymphoid potential [ 11 - 13 ]. (biomedcentral.com)
Hematopoietic stem cells are formed during embryonic development, and serve as the foundation of the definitive blood program for life. (bvsalud.org)

This event requires a special environment, termed the hematopoietic stem cell niche, which provides the protection and signals necessary to carry out the differentiation of cells from HSC progenitors. (wikipedia.org)
Cumano and colleagues' approach was to culture explants containing hematopoietic progenitors isolated from the yolk sac and the embryo proper before the onset of circulation, and to determine their LT reconstitution potential [ 10 ]. (biomedcentral.com)
Indeed, one critical question contemplated at the Workshop was whether tumors derive from organ stem cells that retain self-renewal properties but acquire epigenetic and genetic changes required for tumorigenicity or whether tumor stem cells are proliferative progenitors that acquire self-renewal capacity. (aacrjournals.org)
Our results showed that CP-CML CD34+ progenitors were characterized by significant lower phosphorylation of proteins involved in the regulation of growth and cell survival, such as tyrosine kinases of the Src family and members of STAT family, and by a significant higher phosphorylation of p53 (Ser15), compared to normal CD34+ cells from healthy donors. (oncotarget.com)

This niche relocates from the yolk sac to eventually rest in the bone marrow of mammals. (wikipedia.org)

Many human blood cells, such as red blood cells (RBCs), immune cells, and even platelets all originate from the same progenitor cell, the hematopoietic stem cell (HSC). (wikipedia.org)
The hemangioblast theory, which posits that the RBCs and ECs derive from a common progenitor cell, was developed as researchers observed that receptor knockout mice, such as Flk1-/-, exhibited defective RBC formation and vessel growth. (wikipedia.org)

These studies and others supported the idea that bone cells create the HSC niche, and all the research that elucidated this specialized hematopoietic microenvironment stemmed from these landmark studies. (wikipedia.org)
This balance is in turn regulated by interactions between MK2-IN-1 hydrochloride stem cells and their microenvironment-the so-called niche. (conferencedequebec.org)
Additionally, characterization of the tumor microenvironment such as interactions with immune cells remain largely unknown. (springer.com)
Apart from CSCs, there are a myriad of factors and interactions between various cell types and the tumor microenvironment (TME) that ultimately affect CCA progression. (springer.com)
These data support the notion that the 3D microenvironment created within the microcavity array preserves vital stem cell functions of HPCs more efficiently than conventional co-culture systems. (fairdomhub.org)

However, all developmental processes originate from pluripotent precursor cell types. (amegroups.org)
Specifically, about the paracrine activities of amniotic fluid stem cells, renal stem cells, embryonic stem cells, mesenchymal stem cell, induced pluripotent stem cells as well as other stem cells. (biomedcentral.com)
Bhatia's team spent more than six years delving down to the cellular level to examine what they say are previously overlooked cells that form on the edges of pluripotent stem cell colonies. (longdom.com)

G-CSF, the standard and most widely used agent for this purpose over the past 25 years, mobilizes stem cells from the marrow niche by secretion of neutrophil-associated extracellular proteases which subsequently releases HSPC from their niche22,23. (conferencedequebec.org)
Sulfated polysaccharides (SPs) are anionic carbohydrate polymers synthesized as extracellular or cell wall components by most of the algae and have potent bioactive properties. (brjnmims.org)
Targeted Therapy for Peri-prosthetic Osteolysis Using Macrophage Membrane-encapsulated Human Urine-derived Stem Cell Extracellular Vesicles. (stembook.org)

In the case of hematopoietic stem and progenitor cells, the dormancy of the most primitive HSPC is maintained by the bone marrow niche by means of several key molecular interactions between receptor-ligand pairs1C3. (conferencedequebec.org)

HSPC arterial amplification occurs prior to seeding of secondary hematopoietic tissues and proceeds, in part, through the cell cycle regulator myca (c-myc). (bvsalud.org)
Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)
Epithelial to mesenchymal transition (EMT) is a central mechanism for diversifying the cells found in complex tissues. (bioseek.eu)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)

Transforming growth factor-beta signaling-deficient fibroblasts enhance hepatocyte growth factor signaling in mammary carcinoma cells to promote scattering and invasion. (inabj.org)
Stromal fibroblasts in cancer initiation and progression. (inabj.org)
In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)

Although CD4 T cells are the main targets of HIV infection, macrophages also become infected and resist the cytopathic effects of infection, contributing potentially to HIV reservoir persistence. (nih.gov)
VCAM-1+ macrophages guide the homing of HSPCs to a vascular niche. (amegroups.org)
During this first wave, small number of myeloid cells (e.g., primitive monocytes/macrophages and megakaryocytes) are also generated. (amegroups.org)

Introduction Functions of somatic stem cells are strictly governed by an appropriate balance between self-renewal and differentiation. (conferencedequebec.org)
CSCs take charge of a cancer hierarchy, harboring stem cell-like properties involving self-renewal and aberrant differentiation potential. (bmbreports.org)
The meeting participants were charged with evaluating data suggesting that cancers develop from a small subset of cells with self-renewal properties analogous to organ stem cells. (aacrjournals.org)
The self-renewal and proliferative nature of stem cells raised the hope to fight against various diseases. (biomedcentral.com)
An international collaboration has found for the first time that two new epigenetic regulators, TAF5L and TAF6L, maintain self-renewal of embryonic stem cells. (longdom.com)

If cancer stem cells are relatively refractory to therapies that have been developed to eradicate the rapidly dividing cells within the tumor that constitute the majority of the nonstem cell component of tumors, then they are unlikely to be curative and relapses would be expected. (aacrjournals.org)
Such insights could business lead in turn towards the advancement of book cell therapies predicated on endogenous biologic differentiation potential. (morainetownshipdems.org)
In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
This differential signaling activation network may be addressed by novel targeted therapies aimed at eradicating CML stem cells. (oncotarget.com)

Euro Stem Cells 2019 aims in proclaim knowledge and share new ideas amongst the professionals, industrialists and students from research area of ' Stem Cell Research and Molecular Biology ' to share their research experiences and indulge in interactive discussions at the event. (longdom.com)

However, Ueno and Weissman provided the earliest contradiction to the hemangioblast theory when they saw that distinct ES cells mixed into a blastocyst resulted in more than 1 ES cell contributing to the majority of the blood islands found in the resultant embryo. (wikipedia.org)
Distinct anatomical regions of primitive and definitive hematopoietic development in both the mouse and human. (biomedcentral.com)
We identified both gene and protein markers for three temporally distinct ProSG cell subsets, including a migratory cell population with a transcriptome distinct from the previously defined T1- and T2-ProSG stages. (biologists.com)

For the past 20 years, plenty of studies have suggested that only a small subpopulation of the cancer cells with tumor-initiating capability is the core origin of the tumorigenesis and the subset of cancer cells was named cancer stem cells (CSCs). (bmbreports.org)
In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit^+, Sca1^+ cells. (thermofisher.com)

Cytokines are essential for the function and maintenance of cells, and altered cytokine levels influence not only leukemic cells, but also the normal HSC within the BM. (haematologica.org)

Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
Development of the dorsal aorta is a key step in the establishment of the adult blood-forming system, since hematopoietic stem and progenitor cells (HSPCs) arise from ventral aortic endothelium in all vertebrate animals studied. (bvsalud.org)
Although SDECs lack hematopoietic potential, they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. (bvsalud.org)
All mature blood cell types in the adult animal arise from hematopoietic stem and progenitor cells (HSPCs). (bvsalud.org)

To identify regulators of primitive chronic myeloid leukemia (CML) cells, we performed a high-content cytokine screen using primary CD34 + CD38 low chronic phase CML cells. (haematologica.org)
2 1 There is growing evidence to suggest that primitive CML cells affect the bone marrow (BM) niche, contributing to deregulated cytokine levels. (haematologica.org)

In the cancer stem cell model of tumors, there is a small subset of cancer cells, the cancer stem cells, which constitute a reservoir of self-sustaining cells with the exclusive ability to self-renew and maintain the tumor. (aacrjournals.org)

We establish a three-dimensional (3D) co-culture system based on a custom-made chip, the 3(D)-KITChip, as an in vitro model system of the human hematopoietic stem cell niche. (fairdomhub.org)

Further studies in mice by Cumano and colleagues sought to address the issue of an anatomical origin of the long-term (LT) reconstituting hematopoietic stem cell (HSC) found in adult animals [ 10 ]. (biomedcentral.com)
Overall, they demonstrated that the definitive HSC differs from the primitive HSC by lymphoid potential as well as in their ability to achieve LT reconstitution following transfer into adult recipients. (biomedcentral.com)
Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)

Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)
The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)

Effects of oxygen metabolites/chemokines on hematopoietic stem cell expansion and preservation. (rochester.edu)

A year later, Choi showed that blast cells derived from embryonic stem (ES) cells displayed common gene expression of both hematopoietic and endothelial precursors. (wikipedia.org)
What started as a series of microscopic observations in different animal model systems has since evolved into studies of gene expression and regulation, and subsequent protein-protein interactions, cell surface protein expression profiling, and functional mapping of cell fates. (biomedcentral.com)
This complex process is regulated by the interplay of transcription factors with a chromatin environment, both of which provide the epigenetic information maintaining cell-type specific gene expression patterns. (amegroups.org)
The stimulus for the change of cell fate originates from extrinsic signals which set a cascade of intracellular processes in motion that eventually terminate at the genome leading to changes in gene expression and the development of alternate gene regulatory networks. (amegroups.org)
In the developing embryo, TFs regulating the assembly/disassembly of transcriptional complexes and ultimately gene expression, are directed by complex extrinsic signalling processes which connect all cells within a multi-cellular organism to their environment. (amegroups.org)

The intricate molecular mechanisms involved in the regenerative process of the normal intestine and the identity of putative somatic intestinal stem cells have become clear. (mdpi.com)

We demonstrate that snai2 morphants fail to initiate HSC specification and show defects in the somitic niche of migrating HSC precursors. (bvsalud.org)

Debate and Outcomes Effect on HSPC-niche connections mediated via SDF1-CXCR4 axis Amount?2A displays the adhesion behavior of HSPC towards the surrogate specific niche market model displaying SDF1 seeing that the ligand. (conferencedequebec.org)
VCAM-1, vascular cell adhesion molecule-1. (amegroups.org)

In addition to mobilizing HSPC, the interference with the CXCR4-SDF1 axis has also been proposed as a possible strategy to mobilize malignant stem cells from their protective niche, hence making tumor stem cells even more susceptible to irradiation or chemo- therapy. (conferencedequebec.org)
Several research indicated that seductive get in touch with between CXCR4 portrayed on tumor cells and SDF1 in the specific niche market might represent an integral system for metastatic spread and tumor level of resistance37,38. (conferencedequebec.org)
Cancer stem cells (CSCs) represent a small subset of the tumor population and reside at the apex of the hierarchy [ 9 ]. (springer.com)
A workshop was convened by the AACR to discuss the rapidly emerging cancer stem cell model for tumor development and progression. (aacrjournals.org)
Either mechanism is different from the widely held notion that most cells in a tumor should be competent for tumor formation. (aacrjournals.org)

Snail2 is a zinc-finger transcription factor best known to repress expression of genes encoding cell adherence proteins to facilitate induction of the epithelial-to-mesenchymal transition. (bvsalud.org)
The embryo induced morphologic changes include occurrence of epithelial plaque reaction, stromal compaction and decidualization. (brjnmims.org)

Using single-cell RNA sequencing (RNAseq), we studied the development of ProSG, their SG descendants and testicular somatic cells during the perinatal period in mice. (biologists.com)
We also molecularly defined the development of Sertoli, Leydig and peritubular myoid cells during the perinatal period, allowing us to identify candidate signaling pathways acting between somatic and germ cells in a stage-specific manner during the perinatal period. (biologists.com)

Microenvironmental CXCL12 deletion enhances Flt3-ITD acute myeloid leukemia stem cell response to therapy by reducing p38 MAPK signaling. (stembook.org)

Both StemPro and HPL medium proved to be suitable for clinical application and allowed sufficient cell proliferation. (fairdomhub.org)
BCR-ABL oncogene activates multiple cross-talking signal transduction pathways (STP), such as RAS/MEK/ERK, PI3K/Akt, Wnt and STAT5, contributing to abnormal proliferation of clonal cells. (oncotarget.com)
From this perspective, the aim of this study was to analyze the expression and activation profile of STP involved in the mechanisms of cell proliferation/quiescence and survival of the progenitor CD34+ cells from chronic phase (CP) CML. (oncotarget.com)
In sum, we report that CD34+ cells from CP-CML are characterized by a proteomic and phospho-proteomic profile that promotes quiescence through the inhibition of proliferation and the promotion of survival. (oncotarget.com)

Primitive and definitive hematopoietic development. (biomedcentral.com)

Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm caused by an acquired 9;22-chromosomal translocation in a hematopoietic stem cell (HSC) resulting in the expression of the BCR-ABL1 fusion protein. (haematologica.org)
Chronic Myeloid Leukemia (CML) is a stem cell disease sustained by a rare population of quiescent cells which are to some extent resistant to tyrosine kinase inhibitors (TKIs). (oncotarget.com)

Biologists have found that aging takes a toll on intestinal stem cells and may contribute to increased susceptibility to disorders of the gastrointestinal tract. (longdom.com)

(B) Subset of cell surface markers used in the characterization of this developmental process. (biomedcentral.com)

These hallmarks simplify the complexities of neoplastic disease into a structured rational principles, includes sustaining proliferative signaling, eluding growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genome instability, inflammation, reprogramming energy metabolism and evading immune destruction. (inabj.org)

Background: Stromal cells are a functionally important component of human carcinomas. (immune-source.com)

Western blot analysis showed that stromal cells of lung tumours were characterised by a statistically considerably lower phrase level of the p16 proteins as likened with that in regular lung stromal cells. (immune-source.com)
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

In 1978, after observing that the prototypical colony-forming stem cells were less capable at replacing differentiated cells than bone marrow cells injected into irradiated animals, Schofield proposed that a specialized environment in the bone marrow allows these precursor cells to maintain their cellular reconstitution potential. (wikipedia.org)

Here, we employ a fluorescent reporter system to track and isolate stem-like cancer cells of cholangiocarcinoma cell lines. (springer.com)
Stratton MR. Exploring the genomes of cancer cells: progress and promise. (inabj.org)

Stem Cell Research and Molecular Biology is the latest trending research in many fields especially in Organ cancer: breast, oral, head and neck Cancer Myeloid, lymphoid leukaemia's, cancer treatments etc. (longdom.com)

Anatomy51

Organisms14

Diseases18

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Psychiatry and Psychology1

Phenomena and Processes41

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science2

Named Groups2

Health Care2

MSCs18

Mesenchymal stem1

HSCs10

Differentiate7

Marrow stro1

Tissue15

Stemness3

Transplantation8

Endothelial cells2

Lineages2

Hematopoiesis17

Assays1

Plasticity1

Embryonic development2

Progenitors4

Yolk sac1

Progenitor cell2

Microenvironment5

Pluripotent3

Extracellular3

Bone marrow niche1

Tissues6

Fibroblasts3

Macrophages3

Self-renewal5

Therapies5

20191

Distinct3

Subpopulation2

Leukemic cells1

HSPCs6

Cytokine2

Subset of cancer cells1

Vitro1

Adult3

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Gene expression5

Putative1

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Tumor5

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Markers1

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Lymphoid1