DifferentiationBone marrowProgenitorProgramming hematopoietic stemTissuesStem cellHSCsEndothelialPathwaysMurine hematopoieticTransplantationMyeloid lineageHematopoiesisPrecursorsCellSignaling pathwayTranscriptionalVivoAcute lymphoblGenesStromalVitroMononuclearMiceRegulationGeneticAdultMutationsRegulatorsNotchSystemSeverePlays an important roleDevelopmentBlood cellsMain
Differentiation10
- Here, we show that the emergence of blood is regulated by multiple metabolic pathways that induce or modulate the differentiation toward specific hematopoietic lineages during human EHT. (lu.se)
- Our findings reveal that during EHT, metabolism is a major regulator of primitive versus definitive hematopoietic differentiation. (lu.se)
- The majority of ES clones that expressed lacZ in blood islands also expressed lacZ upon differentiation into hematopoietic cells on OP9 stromal layers. (ox.ac.uk)
- Finally, in proof of principle experiments, we will apply our tool box of immuno reagents against defined HS motifs as an orthogonal system to the well-established cluster of differentiation (CD) system to characterize and define hematopoietic cell lineages. (cancer.gov)
- Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. (rndsystems.com)
- Therefore, we will provide insights into both cell lineage and the molecular mechanisms involved in differentiation. (europa.eu)
- Second, altering the MSC state by inducing differentiation into multiple lineages did not affect the exosomal properties or endocytosis but triggered the expression of lineage-specific genes and proteins in vitro and in vivo respectively. (frontiersin.org)
- Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)
- Lineage promiscuity in hematopoietic differentiation and leukemia. (bdbiosciences.com)
- Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
Bone marrow8
- However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. (nih.gov)
- Rather, fusion of blood cells with different non-haematopoietic cell types is induced by organ-specific injuries or whole-body irradiation, which has been used in previous studies to condition recipients of bone marrow transplants. (diva-portal.org)
- Flow cytometric analysis showed that benzene caused a significant decrease in the number of human hematopoietic stem/progenitor cells in the bone marrow and the number of human leukocytes in the peripheral blood and hematopoietic organs. (elsevierpure.com)
- Low-power view of hematoxylin-eosin-stained bone marrow showing hypocellularity, with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)
- The clinical presentation of patients with aplastic anemia includes signs and symptoms related to the decrease in bone marrow production of hematopoietic cells. (medscape.com)
- Aplastic anemia is a disorder of the hematopoietic stem cell that results in a loss of blood cell precursors, hypoplasia or aplasia of bone marrow, and cytopenias in two or more cell lines (red blood cells, white blood cells, and/or platelets). (msdmanuals.com)
- The term aplastic anemia commonly implies a panhypoplasia of the bone marrow with cytopenias in at least two hematopoietic lineages. (msdmanuals.com)
- While IAEMH is associated with severe anaemia due to intravascular haemolysis caused by red cell invasion, oxidative injury, auto-antibodies, and/or pathogen-haem interaction, IAIMH is associated with haemophagocytic tri-lineage destruction of haematopoietic precursors in the bone marrow. (bvsalud.org)
Progenitor6
- Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. (nih.gov)
- Analysis and genetic manipulation of hematopoietic progenitor cells. (epfl.ch)
- A surrogate human hematopoietic lineage can be established in NOD/Shi-scid/IL-2Rγ null (NOG) mice by transplanting human hematopoietic stem/progenitor cells (Hu-NOG mice). (elsevierpure.com)
- A surrogate human hematopoietic lineage can be established in NOD/Shi-scid/IL-2Rγnull (NOG) mice by transplanting human hematopoietic stem/progenitor cells (Hu-NOG mice). (elsevierpure.com)
- Notch activation in human CD34+ hematopoietic stem/progenitor cells (HSPCs) by treatment with Delta1 ligand has enabled clinically relevant ex vivo expansion of short-term HSPCs. (confex.com)
- Most clusters could be computationally assigned to a defined hematopoietic subpopulation, including progenitor cells (clusters 0 to 4) and a single transcriptionally defined HSC population (cluster 5). (confex.com)
Programming hematopoietic stem1
- Genetic regulatory networks programming hematopoietic stem cells and erythroid lineage specification. (ox.ac.uk)
Tissues3
- However, through lineage fate-mapping we demonstrate that such in vivo fusion of lymphoid and myeloid blood cells does not occur to an appreciable extent in steady-state adult tissues or during normal development. (diva-portal.org)
- Our findings demonstrate that blood cells of the lymphoid and myeloid lineages contribute to various non-haematopoietic tissues by forming rare fusion hybrids, but almost exclusively in response to injuries or inflammation. (diva-portal.org)
- Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. (origene.com)
Stem cell7
- Combinations of MIMs, exemplified by the SCL/LMO2 complexes, which have variable content and binding sites, explain how individual regulators can have different targets in HSCs and erythroid cells and possibly also how HSCs maintain stem cell functions while expressing lineage-affiliated genes at low level, so-called multi-lineage priming. (ox.ac.uk)
- Hematopoietic Stem Cell and Leukaemic Stem Cell environmental stress responses (In vitro biomimicry of hypoxia, normoxia, hypothermia, hyperthermia, hypoglycemia and hyperglycemia): Effect on cellular growth, cell cycle distribution, apoptosis and cellular metabolism. (imperial.ac.uk)
- Mathematical modeling of Hematopoietic Stem Cell and Leukaemic Stem Cell culture systems. (imperial.ac.uk)
- We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
- The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
- His research focus is on haematopoietic stem cell transplantation (HSCT) and translational research of normal and malignant stem cells. (edu.au)
- The precise mechanism remains unclear, but in the majority of acquired cases, the mechanism involves an immune attack on the hematopoietic stem cell. (msdmanuals.com)
HSCs4
- Hematopoietic stem cells (HSCs) emerge from hemogenic endothelial (HE) cells, through endothelial-to-hematopoietic transition (EHT). (lu.se)
- Feed-forward motifs with autoregulation generate forward momentum and also control its rate, which is at its lowest in hematopoietic stem cells (HSCs). (ox.ac.uk)
- Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
- However, sustained engraftment of the expanded cells was not observed after transplantation, suggesting ineffective expansion of hematopoietic stem cells with long-term repopulating activity (LTR-HSCs). (confex.com)
Endothelial1
- Expression trapping: identification of novel genes expressed in hematopoietic and endothelial lineages by gene trapping in ES cells. (ox.ac.uk)
Pathways1
- Kegg pathways include: cytokine-cytokine receptor interaction, Jak- STAT signaling pathway, MAPK signaling pathway, and hematopoietic cell lineage. (cdc.gov)
Murine hematopoietic3
- We have developed a large-scale, expression-based gene trap strategy to perform genome-wide functional analysis of the murine hematopoietic and vascular systems. (ox.ac.uk)
- One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
- While murine hematopoiesis has been considered as resolved science, recent results using barcoding, which allows the tracing of single cells in vivo, clearly show that the topology of the murine hematopoietic tree needs revisions. (europa.eu)
Transplantation2
- The specific medications administered depend on the choice of therapy and whether it is supportive care only, immunosuppressive therapy, or hematopoietic cell transplantation. (medscape.com)
- Knowledge to date on secondary malignancy following hematopoietic cell transplantation for sickle cell disease. (cdc.gov)
Myeloid lineage1
- Recent studies have suggested that regeneration of non-haematopoietic cell lineages can occur through heterotypic cell fusion with haematopoietic cells of the myeloid lineage. (diva-portal.org)
Hematopoiesis4
- During embryonic development, hematopoiesis occurs through primitive and definitive waves, giving rise to distinct blood lineages. (lu.se)
- Fig. 3: Dissection of native human hematopoiesis with multimodal cell state inference and mtDNA-based lineage tracing. (nature.com)
- With this method, we will provide the first in vivo description of the human hematopoietic tree and identify new progenitors and key molecular factors involved in human hematopoiesis. (europa.eu)
- Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
Precursors1
- Enforcement of γδ-lineage commitment by the pre-T-cell receptor in precursors with weak γδ-TCR signals. (sunnybrook.ca)
Cell4
- The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. (nih.gov)
- This requirement strongly limits the number of solutions or entiation and lineage-specification, programmed cell death, and ``states'' for the system. (lu.se)
- People who need revaccination are those who are moderately or severely immunocompromised who received COVID-19 vaccine dose(s) during treatment with B-cell-depleting therapies over a limited period or who received COVID-19 vaccine dose(s) prior to or during treatment involving hematopoietic cell transplant or chimeric antigen receptor (CAR)-T-cell therapy. (cdc.gov)
- Prognostic impact of pre-transplant chromosomal aberrations in peripheral blood of patients undergoing unrelated donor hematopoietic cell transplant for acute myeloid leukemia. (cdc.gov)
Signaling pathway1
- Adult Acute Myeloid Leukemia with the KMT2A-Mixed Lineage Leukemia T10 Fusion: An Analysis of 10 Cases Showed Common Features and Frequent Mutations in the RAS Signaling Pathway. (cdc.gov)
Transcriptional1
- Mouse prenatal platelet-forming lineages share a core transcriptional program but divergent dependence on MPL. (edu.au)
Vivo3
- In both in vitro and in vivo settings, steering pyruvate use toward glycolysis or OXPHOS differentially skews the hematopoietic output of HE cells toward either an erythroid fate with primitive phenotype, or a definitive lymphoid fate, respectively. (lu.se)
- Notch signals are required for in vitro but not in vivo maintenance of human hematopoietic stem cells and delay the appearance of multipotent progenitors. (sunnybrook.ca)
- This phenomenon occurs in otherwise unmanipulated murine marrow in vivo, resulting in circulating platelets that bear membrane from non-megakaryocytic hematopoietic donors. (elifesciences.org)
Acute lymphobl1
- Asynchronous antigen expression in B lineage acute lymphoblastic leukemia. (bdbiosciences.com)
Genes2
- MIMs combined with cross-antagonism describe the relationship between PU.1 and GATA-1 and between two of their target genes, Fli-1 and EKLF, with victory for GATA-1 and EKLF leading to erythroid lineage specification. (ox.ac.uk)
- Expression and sequence analysis of representative clones suggest that this approach will be useful for identifying and mutating novel genes expressed in the developing hematopoietic and vascular systems. (ox.ac.uk)
Stromal1
- Both hematopoietic and non-hematopoietic (stromal) cells can, under certain circumstances, differentiate into cells of various neuronal and glial lineages. (nih.gov)
Vitro1
- Human CD8 T cells generated in vitro from hematopoietic stem cells are functionally mature. (sunnybrook.ca)
Mononuclear1
- Osteoclasts are bone resorbing, multinucleate cells that differentiate from mononuclear macrophage/monocyte-lineage hematopoietic precursor cells. (jci.org)
Mice4
- Here, we first evaluated the toxic response of human-like hematopoietic lineage in NOG mice to a representative toxic agent, benzene. (elsevierpure.com)
- A comparison of the degree of benzene-induced hematotoxicity in donor-derived hematopoietic lineage cells within Mo-NOG mice indicated that the toxic response of Hu-NOG mice reflected interspecies differences in susceptibilities to benzene. (elsevierpure.com)
- Physiologic aging in both humans and mice leads to permanent changes in LT-HSC function, such as myeloid-biased hematopoietic output ( Akunuru and Geiger, 2016 ). (biorxiv.org)
- Homozygous null mice are viable and healthy with no abnormalities detected in any of the hematopoietic lineages. (jax.org)
Regulation1
- Context-dependent regulation of hematopoietic lineage choice by HEBAlt. (sunnybrook.ca)
Genetic1
- Since these mutations occur in utero and are very hard to study in humans, we used mouse models that express the same genetic mutations to investigate their impact on the blood or hematopoietic system. (lu.se)
Adult1
- BLUEPRINT RNA-seq data for common cells in the haematopoietic lineages, from adult and cord blood samples. (ega-archive.org)
Mutations1
- Some of these mutations, such as (mixed-lineage leukemia) MLL fusions, are highly aggressive and can generate leukemia very quickly. (lu.se)
Regulators1
- 2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
Notch1
- Direct comparison of Dll1- and Dll4-mediated Notch activation levels shows differential lymphomyeloid lineage commitment outcomes. (sunnybrook.ca)
System1
- This knowledge will allow us to test whether cells with different origins have different functions, a key question in immunology in particular during infection and inflammation, and it will help design ways of therapeutically manipulating the hematopoietic system. (europa.eu)
Severe1
- Similarly, destruction of neonatally abundant pluripotent stem cells would likely have a more pervasive outcome than destruction of The value of incorporating immunologic appeared more severe and/or persistent when single lineages or differentiated cells that pre- data for the toxicologic assessment of drugs, the exposure occurred perinatally when com- dominate in adults. (cdc.gov)
Plays an important role1
- In particular, it plays an important role in development of hematopoietic stem cells, early blood cells that have the potential to develop into all types of mature blood cells such as white blood cells, red blood cells, and platelets. (medlineplus.gov)
Development1
- These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage. (nih.gov)
Blood cells1
- Discovery of new hematopoietic tissue and discovery of multinucleated giant blood cells in D ananassae. (nottingham.ac.uk)
Main1
- Morphological recognition of main hematopoietic lineages. (epfl.ch)