• Hematopoiesis is maintained throughout life from the hematopoietic stem cell niche in which hematopoietic stem cells and lineage-specific hematopoietic progenitors (HSPCs) reside and regulate hematopoiesis . (bvsalud.org)
  • The Hematopoiesis and Hematopoietic Stem Cell Biology program focuses on understanding the basic cellular and molecular mechanisms underlying the production and function of blood cells in health and disease. (nih.gov)
  • Major areas of interest include the basic mechanisms involved in regulating the production and terminal development of blood cells, referred to as hematopoiesis. (nih.gov)
  • These results demonstrate that Gal-3 is a negative regulator of cell-cycling in HSCs and plays a crucial role in adult hematopoiesis to prevent HSC exhaustion. (nature.com)
  • This clonal model of heart lineage diversification would be analogous to hematopoiesis, in which a single hematopoietic stem cell can generate all of the blood cell lineages. (nih.gov)
  • Clonal hematopoiesis (CH) is defined as the acquisition of somatic mutations in blood cells and is associated with smoking and exposure to genotoxic stimuli. (cdc.gov)
  • Hematopoietic stem cells (HSCs) and hematopoiesis are among the tissues/organs most sensitive to radiation injury and contribute to many of the manifestations of acute radiation injury, including bleeding, infection, and bone marrow failure. (biomedcentral.com)
  • In 10-20% of healthy individuals over age 70, the acquisition of pre-leukemic mutations in hematopoietic stem cells (HSCs) results in the dominance of a small number of HSC-derived clones, a process termed Clonal Hematopoiesis of Indeterminate Potential (CHIP) 6 , 7 . (nature.com)
  • The combination of prospective identification/isolation of bone marrow progenitors and quantitative RT-PCR is a powerful tool to understand the molecular mechanism underlying hematopoiesis. (researchsquare.com)
  • Extramedullary tissues harbor reservoirs of HSC/MPPs and non-proliferative progenitors without ongoing hematopoiesis. (kumamoto-u.ac.jp)
  • Collectively, we identify extramedullary lineage-primed HSPC reservoirs that are non-proliferative in situ and report involvement of splenic HSPCs during demand-adapted hematopoiesis. (kumamoto-u.ac.jp)
  • When introduced into c-Kit(high)Sca-1(+)Lineage(-) cells, FIP1L1-PDGFRalpha conferred cytokine-independent growth on these cells and enhanced their self-renewal, whereas it did not immortalize common myeloid progenitors in in vitro replating assays and transplantation assays. (nih.gov)
  • and hematopoietic stem cell transplantation and the development of techniques for establishing stable hematopoietic chimeras in order to treat heritable blood diseases. (nih.gov)
  • For pediatric patients with refractory cytopenia, certain cytogenetic abnormalities, or malignant transformation, hematopoietic stem cell transplantation (HSCT) from a matched related or unrelated donor early in the course of the disease is the treatment of choice. (medscape.com)
  • Humanized mice (hu mice) are based on the transplantation of hematopoietic stem and progenitor cells into immunodeficient mice and have become important pre-clinical models for biomedical research. (nih.gov)
  • They have certain immunomodulatory, immunosuppressive, and antimicrobial properties that enable them to have several therapeutic and clinical applications including: treatment of autoimmune disorders, role in hematopoietic stem cell transplantation and regenerative medicine, as well as treatment of various infections and their associated complications such as septic shock and acute respiratory distress syndrome. (intechopen.com)
  • The defect is intrinsic to individual HSCs and their progeny are skewed toward proliferation and differentiation as evidenced by single cell and transplantation assays. (altmetric.com)
  • Myelosuppressive drugs (eg, hydroxyurea ), stem cell transplantation, and interferon alfa are also sometimes used. (msdmanuals.com)
  • Successful blood and marrow transplantation (BMT) requires the infusion of a sufficient number of hematopoietic stem/progenitor cells (HSPCs), capable of both homing to the bone marrow and regenerating a full array of hematopoietic cell lineages with early and late repopulating ability in a timely fashion. (mycancergenome.org)
  • Modulation of AHR in human hematopoietic cells in vitro is a promising tool to mediate development of terminal hematopoietic cell populations with significant clinical implications to generate cells suitable for antitumor immunotherapy and bone marrow transplantation. (escholarship.org)
  • Recently, long-term transplantation experiments point toward a clonal diversity model of hematopoietic stem cells. (medicalxpress.com)
  • Thus, this chapter focuses on the structural (clastogenicity) and numerical (aneugenicity) form of CA analysis in lineage-specific HSPCs comprised of myeloid, erythroid and lymphoid lineages.In this protocol, we describe how to perform CA analysis in lineage-specific HSPCs derived from freshly isolated mouse bone marrow cells (MBMCs) using the combined techniques of colony-forming unit (CFU) and karyotyping . (bvsalud.org)
  • Hematopoietic stem cells (HSCs) in adult bone marrow (BM) are usually maintained in a state of quiescence. (nature.com)
  • This event explains the presence of multiple derangements observed in the bone marrow that involve several cell lineages. (medscape.com)
  • As the affected cell lines continue to divide and to provide the marrow with dysplastic cells, bone marrow dysfunction becomes apparent. (medscape.com)
  • AHR ligands modulate the frequency of mature hematopoietic cells in the bone marrow and periphery, while HSPCs from mice lacking AHR (AHR KO) have increased proliferation. (nih.gov)
  • IRF8 regulates development of myeloid lineage cells: It is expressed in a series of myeloid progenitors in bone marrow that are derived from hematopoietic stem cells. (nih.gov)
  • We therefore characterized leukocyte reconstitution in NSG mice, which were sublethally irradiated and transplanted with human cord blood-derived CD34+ cells at newborn age, longitudinally in peripheral blood and, for more detailed analyses, cross-sectionally in peripheral blood, spleen and bone marrow at different time points. (nih.gov)
  • Human cell chimerism in spleen and bone marrow was maintained over time. (nih.gov)
  • Notably, human cell chimerism in peripheral blood and spleen as well as bone marrow positively correlated with each other. (nih.gov)
  • Importantly, the percentage of more primitive hematopoietic stem cells (HSCs) in bone marrow was maintained over time. (nih.gov)
  • Mesenchymal stem cells are heterogenous adult multipotent stromal cells that can be isolated from various sources including: bone marrow, peripheral blood, umbilical cord blood, dental pulp, and adipose tissue. (intechopen.com)
  • It is a receptor expressed on immature B lymphocytes, hematopoietic progenitors and stem cells in adult bone marrow, fetal liver, embryonic yolk sac. (biolegend.com)
  • CD93 expression level on splenic immature/transitional B cells is much lower than in bone marrow. (biolegend.com)
  • It is reinduced during plasma cell differentiation and plays an important role in maintaining plasma cells in bone marrow niches. (biolegend.com)
  • CD93 plays an important role in maintaining of plasma cells in bone marrow niches. (biolegend.com)
  • Micro-environmental sensing by bone marrow stroma identifies IL-6 and TGFβ1 as regulators of hematopoietic ageing. (ox.ac.uk)
  • B-cell-autonomous somatic mutation deficit following bone marrow transplant. (ox.ac.uk)
  • The bcr-abl oncoprotein has uncontrolled tyrosine kinase activity, which deregulates cellular proliferation, decreases adherence of leukemia cells to the bone marrow stroma, and protects leukemic cells from normal programmed cell death (apoptosis). (msdmanuals.com)
  • CML ensues when an abnormal pluripotent hematopoietic progenitor cell initiates excessive production of all myeloid lineage cells, primarily in the bone marrow but also in extramedullary sites (eg, spleen, liver). (msdmanuals.com)
  • TXN administration led to enhanced recovery of peripheral blood cell counts, bone marrow cellularity, and HSC population as measured by c-Kit + Sca-1 + Lin - (KSL) cells, SLAM + KSL cells and CFUs. (biomedcentral.com)
  • TXN treatment reduced cell senescence and radiation-induced double-strand DNA breaks in both murine bone marrow lineage-negative (Lin - ) cells and primary fibroblasts. (biomedcentral.com)
  • Omidubicel utilizes the small molecule nicotinamide (NAM), as an epigenetic approach to inhibit differentiation and to increase the migration, bone marrow (BM) homing and engraftment efficiency of hematopoietic progenitor cells (HPC) expanded in ex vivo cultures. (mycancergenome.org)
  • Initial characterization of the human fetal bone marrow and adult adipose tissues revealed that a subpopulation of CD146+ perivascular cells was capable of supporting the self-renewal of transplantable human cord blood HSCs ex vivo. (escholarship.org)
  • Hematopoietic stem cells (HSCs) are rare cells found in the bone marrow that produce red blood cells, white blood cells, and platelets. (medicalxpress.com)
  • however they also function as a crucial component in both innate and acquired immune responses1 as well as solid tumor and leukemia progression.2 3 Mast cells delineate from hematopoietic stem cells (HSCs) in the Mouse monoclonal to SLC22A1 bone marrow but unlike other blood cells enter circulation as progenitors. (immune-source.com)
  • Harvest bone marrow (BM) cells and spleen cells and resuspend them in 1×PBS/2%FCS in 50 ml conical tubes. (researchsquare.com)
  • The TER-119 antibody reacts with murine TER119 (Ly-76), an ~52 kDa protein associated with glycophorin A on the surface of cells of the erythroid lineage in embryonic yolk sac, fetal and newborn liver, adult bone marrow, peripheral blood, and lymphoid organs. (stemcell.com)
  • In adult mice, TER119 is found on ~20 - 25% of bone marrow cells and ~2 - 3% of splenocytes. (stemcell.com)
  • A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, followed by goat antimouse IgG, FITC. (stemcell.com)
  • Hematopoietic growth factors are a family of glycoproteins responsible for the proliferation and differentiation of hematopoietic progenitor cells in the bone marrow. (lecturio.com)
  • Expansion of the sagittal suture induces proliferation of skeletal stem cells and sustains endogenous calvarial bone regeneration. (amedeo.com)
  • Rare hematopoietic stem and progenitor cell (HSPC) pools outside the bone marrow (BM) contribute to blood production in stress and disease but remain ill-defined. (kumamoto-u.ac.jp)
  • Kaplan also sponsored me to spend the 4th'5th year 9 month interval with Gowans in Oxford, where I developed intrathymic and intra bone marrow radioactive nucleoside labeling of cells to determine their lineage potential and migration potential. (ipscell.com)
  • MDS is a clonal disorder of myeloid stem cells. (medscape.com)
  • 19. Synchronous FIP1L1-PDGFRA-positive chronic eosinophilic leukemia and T-cell lymphoblastic lymphoma: a bilineal clonal malignancy. (nih.gov)
  • In follow-up assessments of clinical trial patients receiving lentiviral vector-based gene therapy for treatment of X-linked severe combined immunodeficiency, he has utilized iPSCs derived from post-treatment patient hematopoietic stem cells to study the effects of cryptic transcript splicing from the therapeutic lentiviral vector on aberrant clonal hematopoietic outgrowth after hematopoietic stem cell transplant, to develop vector safety modifications that eliminate cryptic splice sites. (nih.gov)
  • Moreover, our results show that clonal expansion of progenitor cells provides a window in which collaborating mutations can accumulate to drive disease progression. (altmetric.com)
  • Chronic myeloid leukemia (CML) occurs when a pluripotent stem cell undergoes malignant transformation and clonal myeloproliferation, leading to a striking overproduction of mature and immature granulocytes. (msdmanuals.com)
  • Fig. 1: MutaSeq for high-quality single-cell RNA-seq data with clonal information. (nature.com)
  • Conditional overexpression of Gal-3 in mouse HSCs under the transcriptional control of Tie2 or Vav1 promoters (Gal-3 Tg) causes cell cycle retardation via induction of p21. (nature.com)
  • Conversely, the cell cycle of long-term repopulating HSCs (LT-HSCs) in Gal-3-deficient (Gal-3 -/- ) mice is accelerated, resulting in their exhaustion. (nature.com)
  • Most studies have shown that extrinsic signals from niche cells affect HSCs by influencing cell cycle-related molecules in HSCs. (nature.com)
  • Activation of NOTCH signaling in human hematopoietic stem/progenitor cells (HSPCs) by treatment with an engineered Delta-like ligand (DELTA1 ext-IgG [DXI]) has enabled ex vivo expansion of short-term HSPCs, but the effect on long-term repopulating hematopoietic stem cells (LTR-HSCs) remains uncertain. (nih.gov)
  • This study revealed that AHR KO mice have an increased proportion of myeloid-biased HSCs and myeloid-biased multipotent progenitor (MPP3) cells. (nih.gov)
  • Using hematopoietic-specific conditional AHR knockout mice (AHRVav1) revealed that increased frequency of myeloid-biased HSCs and myeloid-biased progenitors is driven by AHR signaling that is intrinsic to the hematopoietic compartment. (nih.gov)
  • Quantitative analysis of HSC-derived clones was used to model the fate choices of normal and JAK2-mutant HSCs and indicates that JAK2V617F reduces self-renewal of individual HSCs but leaves progenitor expansion intact. (altmetric.com)
  • This conclusion is supported by paired daughter cell analyses, which indicate that JAK2-mutant HSCs more often give rise to two differentiated daughter cells. (altmetric.com)
  • To induce differentiation toward hematopoietic stem cells (HSCs) in vitro, ESCs were cultured in methylcellulose with stem cell factor, interleukin (IL)-3, and IL-6. (rupress.org)
  • Protection and/or mitigation of hematopoietic stem cells (HSCs) from radiation injury is an important goal in the development of medical countermeasure agents (MCM). (biomedcentral.com)
  • Hematopoietic stem cells (HSCs) are characterized by their ability to self-renew and contribute to multi-lineage differentiation, critical functions that ensure homeostatic production of all blood cells throughout life. (escholarship.org)
  • My goal was to reconstruct an optimal hematopoietic niche from mesodermal differentiation of human pluripotent stem cells (hPSC) to facilitate the maintenance of functional HSCs. (escholarship.org)
  • Hematopoietic stem cells ( HSCs ) are multipotent stem cells that give rise to all the blood cell types including myeloid (monocytes and macrophages, neutrophils, basophils, eosinophils, erythrocytes, megakaryocytes/platelets, dendritic cells), and lymphoid lineages (T-cells, B-cells, NK-cells). (medicalxpress.com)
  • HSCs constitute 1:10.000 of cells in myeloid tissue. (medicalxpress.com)
  • In acute myeloid leukemia (AML), leukemic stem cells (LSCs) underlie mortality but are difficult to isolate due to their low abundance and high similarity to healthy hematopoietic stem cells (HSCs). (nature.com)
  • Here, we demonstrate that LSCs, HSCs, and pre-leukemic stem cells can be identified and molecularly profiled by combining single-cell transcriptomics with lineage tracing using both nuclear and mitochondrial somatic variants. (nature.com)
  • We focussen ons vooral op het begrijpen van de oorsprong van HSCs tijdens de embryonale ontwikkeling, de mechanismen die betrokken zijn bij het genereren en expanderen van HSCs, de samenstelling en functie van de verschillende HSC niches tijdens maturatie, en de cellulaire en moleculaire kenmerken van HSCs en hun voorlopers. (hubrecht.eu)
  • The ability to isolate, renew and differentiate Isl1+ precursors from post-natal/embryonic hearts and from ES cells represents a powerful cell-based system which allows the characterization of signaling pathways controlling formation, renewal and lineage specification of cardiovascular progenitors. (nih.gov)
  • Since this master cardiovascular stem cell can be cloned from embryonic stem cells, this new finding has significant implications both for the study of the heart development, drug discovery, and disease target identification, and for longer term potential therapeutic application of the cells to repair and replace damaged heart, pacemaker, and vascular tissue. (nih.gov)
  • CD34 is expressed on immature haematopoietic stem/progenitor cells, capillary endothelial cells, embryonic fibroblasts and rare glial cells in nervous tissue. (ihcworld.com)
  • Results: Studies were undertaken to evaluate and compare the transfection efficacy of several chemical reagents to that of the electroporation/nucleofection system using both adherent cells (primary and transformed airway epithelial cells and primary fibroblasts as well as embryonic stem cells) and cells in suspension (primary hematopoietic stem/progenitor cells and lymphoblasts). (harvard.edu)
  • While it is commonly accepted as an aging-related phenomenon, senescence might happen also during the embryonic development with the biological meaning of replacing transient structures or specific cell types with other ones [ 3 - 5 ]. (hindawi.com)
  • Establishment of human cell lines from embryonic ear tissue. (nih.gov)
  • A single embryonic stem cell (ESC) line can be repetitively cryopreserved, thawed, expanded, and differentiated into various cellular components serving as a potentially renewable and well-characterized stem cell source. (rupress.org)
  • We harnessed the opportunities provided by the zebrafish model system and our prior characterization and validation of as a mast cell specific marker Dipyridamole to conduct a comprehensive series of embryonic in vivo studies to assess the role of genes in vertebrate mast cell development. (immune-source.com)
  • DPPA5A suppresses the mutagenic TLS and MMEJ pathways by modulating the cryptic splicing of Rev1 and Polq in mouse embryonic stem cells. (amedeo.com)
  • Recently identified BLast Colony Forming Cells (BL-CFCs) from in vitro differentiated embryonic stem (ES) cells represent the common progenitor of hematopoietic and endothelial cells, the hemangioblast. (wustl.edu)
  • This Spotlight discusses recent advent of single cell experimental and computational methods, and key insights from applying these methods to the study of mouse embryonic development. (biologists.com)
  • During mouse development ( Fig. 1 ), inner cell mass (ICM) cells of the blastocyst differentiate and contribute to the epiblast and the extra-embryonic endoderm lineages. (biologists.com)
  • At gastrulation, the multipotent epiblast cells differentiate into the three germ layers: the ectoderm, mesoderm (including embryonic and extra-embryonic cell lineages) and endoderm. (biologists.com)
  • The derivatives of these lineages are regionalized in domains defined by their position along the embryonic body axes, i.e. anterior-posterior, medial-lateral and dorsal-ventral patterning. (biologists.com)
  • [ 7 , 8 ] Transfection of CD34 + HSC progenitors with miR-155 precursors results in a decrease in the number of myeloid and erythroid progenitors, suggesting that this miRNA has a role in normal myelopoesis and erythropoiesis (Figure 2). (medscape.com)
  • CD34 appears to be expressed at its highest level on the earliest progenitors, and to decrease progressively with maturation. (ihcworld.com)
  • CD34 is a stage-specific, rather than a lineage-specific, leucocyte differentiation antigen. (ihcworld.com)
  • Omidubicel is a cryopreserved stem/progenitor cell based product comprised of: Ex vivo expanded, umbilical cord blood-derived hematopoietic CD34 + progenitor cells (cultured fraction (CF)) the non-cultured cell fraction of the same Cord Blood Unit (CBU) (Non-cultured Fraction (NF)) consisting of mature myeloid and lymphoid cells. (mycancergenome.org)
  • AHR hyperactivation following the induction of CD34 megakaryocyte-erythroid progenitors skews developed toward erythroid lineages, whereas AHR inhibition supports platelet production. (escholarship.org)
  • Immunogen: Blast cells from a chronic myeloid leukemia patient were used as the immunogen for this CD34 antibody. (m-entrepreneurship.com)
  • Recent advances in adipogenesis had provided insights into understanding of the complex cues for influencing the cytoarchitecture, epigenomic remodeling, signaling pathways and transcription regulators on gene actions for both white and brown adipogenic progression from mesenchymal stem cells to matured committed adipocytes. (scirp.org)
  • Cell-biology-related research on preadipocyte (adiposal-mesenchymal cell) differentiation laid the foundation for discoveries of adipose-derived cells (both in stromal vascular fraction, or SVF, and adipose-derived stromal/stem cells, or ASCs), which had become an increasing interest to both stem cell biologists and clinicians because of their potential for angiogenesis and suppression of inflammation for tissue engineering and treatments. (scirp.org)
  • Although more success has been achieved in preclinical trials on the use of mesenchymal stem cells in animal models than in human clinical trials, particularly in septic shock and Chagas disease, more progress has been made in both disorders after the recent use of specific sources and certain doses of mesenchymal stem cells. (intechopen.com)
  • The clinical application of mesenchymal stem cells in the treatment of several diseases still faces real challenges that need to be resolved. (intechopen.com)
  • The following book chapter will be an updated review on the role of mesenchymal stem cells in various infections and their complications. (intechopen.com)
  • High throughput sequencing revealed the presence of mesodermal progenitors with trilineage (hematopoietic, endothelial and mesenchymal) potential during hPSC differentiation and uncovered the lineage bifurcation of hematopoietic and mesenchyme early on in separated bipotent populations. (escholarship.org)
  • The hPSC-derived mesenchymal populations were further studied in relation to their ability to promote or inhibit HSC maintenance, leading to the discovery of a population that was phenotypically, functionally and molecularly similar to primary human perivascular cells of the HSC niche. (escholarship.org)
  • Loss of regulation of protein synthesis and turnover underpins an attenuated stress response in senescent human mesenchymal stem cells. (amedeo.com)
  • IL-3 is a pleiotropic cytokine that can stimulate proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors (1, 2). (rndsystems.com)
  • Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. (m-entrepreneurship.com)
  • Recent studies have now revealed that different cardiac cell types arise from a common multipotent Isl1+ progenitor. (nih.gov)
  • The hematopoietic tissue contains cells with long-term and short-term regeneration capacities and committed multipotent, oligopotent, and unipotent progenitors. (medicalxpress.com)
  • Second, extramedullary stem cells/multipotent progenitors (HSC/MPPs) from spleen, PB and mPB share a common transcriptional signature and increased abundance of lineage-primed subsets compared to BM. (kumamoto-u.ac.jp)
  • He has developed efficient iPSC reprogramming protocols using small volumes of patient blood samples or cryopreserved mononuclear cells as well as developing and utilizing iPSC differentiation protocols for production of specific cell lineages, including fibroblasts, neural stem cells, neurons, astrocytes, hematopoietic stem/progenitor cells, monocytes/macrophages, dendritic cells, and microglia. (nih.gov)
  • They are especially interested in identifying those factors that mediate the initial determination and proliferation of hematopoietic stem cells, and progenitor cells for erythroid and lymphoid lineages. (uclahealth.org)
  • The FIP1L1-PDGFRA protein produced from the fusion gene has the function of the normal PDGFRA protein, which stimulates signaling pathways inside the cell that control many important cellular processes, such as cell growth and division (proliferation) and cell survival. (medlineplus.gov)
  • Chien has conducted groundbreaking research focused on the molecular pathways of cardiac development and disease, much of which has been published in the top tier journals in the field of biomedical science, including Cell, Science and Nature. (nih.gov)
  • It promotes LY6C+ monocyte macrophage lineage, while suppressing neutrophil pathways. (nih.gov)
  • The signaling pathways stimulated by the PDGFRA protein control many important cellular processes such as cell growth and division (proliferation) and cell survival. (nih.gov)
  • As a result, the signaling pathways are constantly turned on (constitutively activated), which increases the proliferation and survival of cells. (nih.gov)
  • As a result, the PDGFRA protein and the signaling pathways are constitutively activated, which increases cell proliferation and survival, leading to tumor formation. (nih.gov)
  • By comparing the proteomic and transcriptomic changes in human hematopoietic stem/progenitor cells (HSPCs) and lineage-committed erythroid progenitors (ProEs), and uncover pathways related to mitochondrial biogenesis enhanced through post-transcriptional regulation. (nih.gov)
  • Distinct myeloid progenitor-differentiation pathways identified through single-cell RNA sequencing. (ox.ac.uk)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • We used the opportunities afforded by the zebrafish to determine upstream pathways regulating mast cell development in vivo and identify their cellular origin. (immune-source.com)
  • Meanwhile, HSPCs behavior is modulated by both cell intrinsic (e.g., transcriptional factors) and cell extrinsic (e.g., cytokines ) factors. (bvsalud.org)
  • Moreover, it has been reported that chromosomal aberration (CA) in HSPCs following exposure to carcinogenic or genotoxic agents can initiate leukemia stem cells (LSCs) formation which lays a fundamental mechanism in leukemogenesis. (bvsalud.org)
  • Despite reported studies concerning the chromosomal integrity in HSPCs, CA analysis in lineage-specific HSPCs remains scarce. (bvsalud.org)
  • This indicates a need for a laboratory technique that allows the study of CA in specific HSPCs subpopulations comprising differential hematopoietic lineages. (bvsalud.org)
  • Prior to CA analysis , lineage-specific HSPCs for myeloid, erythroid, and lymphoid were enriched through colony-forming unit (CFU) assay. (bvsalud.org)
  • About 6 to 14 days of cultures are required depending on the type of HSPCs lineage. (bvsalud.org)
  • Then, the CA focusing on clastogenicity and aneugenicity anomalies in respective HSPCs lineage for myeloid, erythroid and Pre-B lymphoid were investigated. (bvsalud.org)
  • We believe our protocol offers a significant contribution to be utilized as a reference method for chromosomal analysis in lineage-specific HSPCs subpopulations. (bvsalud.org)
  • The core function of hematopoietic stem and progenitor cells (HSPCs) is to provide lifelong production of all lineages of the blood and immune cells. (nih.gov)
  • Here, we study the immediate early transcriptional and cis-regulatory responses to TPO in hematopoietic stem/progenitor cells (HSPCs) and use this paradigm of cytokine signaling to chromatin to dissect the relation between cis- regulatory activity and chromatin architecture. (cam.ac.uk)
  • We show that TPO profoundly alters the transcriptome of HSPCs, with key hematopoietic regulators being transcriptionally repressed within 30 minutes of TPO. (cam.ac.uk)
  • Our current focus is on the epigenetic regulation of hematopoietic stem cell (HSC) and progenitor cell lineage commitment in three contexts: (1) normal blood development and maintenance, (2) alterations that occur during the process of aging, and (3) alterations that occur during the process of transformation giving rise to leukemia. (jax.org)
  • The first choice during lineage commitment appears to split myeloid from lymphoid development. (nih.gov)
  • We aim to understand how lymphoid and myeloid commitment is transcriptionally defined and whether the lineage of origin translates into specialized functional properties across different subsets. (nih.gov)
  • These findings elucidated lineage commitment events during early human embryogenesis and informed strategies to optimize the therapeutic development of cell lineages from hPSC. (escholarship.org)
  • They Dipyridamole only complete maturation in resident cells which hinders accurate lineage tracing research in traditional mammalian models greatly.1 We've been utilizing the zebrafish magic size to review mast cell advancement and specifically the transcriptional regulation of mast cell lineage commitment. (immune-source.com)
  • Access to this initial cell population committed to the hematopoietic lineage provides a unique opportunity to characterize hematopoietic commitment events. (wustl.edu)
  • FIP1L1-PDGFRalpha also promoted eosinophil development from common myeloid progenitors. (nih.gov)
  • 6. The EOL-1 cell line as an in vitro model for the study of FIP1L1-PDGFRA-positive chronic eosinophilic leukemia. (nih.gov)
  • Through clone-splitting experiments, where the fates of siblings from a single cell are assessed independently (for example, in two separate wells in vitro or transferred to two recipients in vivo ), we have determined that this heterogeneous fate is often 'imprinted' (Naik et al. (edu.au)
  • The burgeoning preclinical and clinical experience with human adult adipose-derived cells appears to be promising but interpretation of the current literature with in vitro studies, translational research and FDA-registered investigations emphasizes the need for standardized methods to advance basic science knowledge and beneficial safe clinical outcomes. (scirp.org)
  • Exposure of lymphoblastoid cells to WTC particulate matter (WTC-PM) led to dysregulation of DNA replication at common fragile sites in vitro. (cdc.gov)
  • We emphasize approaches to modulate AHR throughout human hematopoietic development in vitro to support the production of clinically relevant blood products suitable for patient care. (escholarship.org)
  • Een grote uitdaging in het veld van stamcelonderzoek is om grote hoeveelheden van deze zeldzame cellen te genereren in vitro voor onderzoek en klinisch gebruik. (hubrecht.eu)
  • Cellular barcoding is a technology that allows the tracking of fate for hundreds of single stem cells simultaneously. (edu.au)
  • We have used the technique to decipher the complexity of their individual fate and to reconstruct single cell lineage decisions in haematopoiesis (Naik, Nature, 2013). (edu.au)
  • While cellular barcoding can determine the fate of many single cells in vivo , it cannot provide information on the shape of the 'family tree' where a single stem cell generates multiple cell types. (edu.au)
  • We are tackling the nature of these molecular programs by performing high throughput single cell RNA sequencing - a relatively new but powerful technique - that can reveal single cell resolution differences that may account for differences in fate. (edu.au)
  • The Trowbridge laboratory studies cell fate regulation within the hematopoietic system. (jax.org)
  • Epigenetic programming defines haematopoietic stem cell fate restriction. (ox.ac.uk)
  • We are a very technology-driven lab with the philosophy that studying single cells at different functional and molecular levels, and integrating this information, will reveal the mechanisms behind their fate specification in health and disease. (edu.au)
  • Combined, we anticipate this suite of tools will allow us to gain a high-resolution understanding into the principles and molecular mechanism governing development and the establishment of lineage fate in normal and cancerous tissues, with view to therapeutic interventions. (edu.au)
  • Aberrant Notch signaling is associated with a wide range of human disorders from developmental syndromes to cancer.13 Notch signaling is involved in the fate determination of a variety of cell types including hematopoietic cells where it participates in differentiation proliferation and apoptosis.14 In mammals the Notch pathway consists of 4 Notch genes (Notch1-4) which encode transmembrane receptor proteins. (immune-source.com)
  • To date these links between the Notch pathway and mast cells have been identified but a detailed interrogation of the role of Notch signaling in contributing to mast cell fate has not been previously undertaken in vivo. (immune-source.com)
  • Mechanosensitive stem cell fate choice is instructed by dynamic fluctuations in activation of Rho GTPases. (amedeo.com)
  • PGE(2) alters chromatin through H2A.Z-variant enhancer nucleosome modification to promote hematopoietic stem cell fate. (amedeo.com)
  • Furthermore, in vivo antagonism of the AHR led to a 2.5-fold increase in the number of MPP3 cells and promoted myeloid-biased differentiation. (nih.gov)
  • Macrophages from CD93 (-/-) mice had a significant phagocytic defect in the clearance of apoptotic cells in vivo , indicating CD93 may contribute to the in vivo clearance of dying cells. (biolegend.com)
  • It may play a role in uptake of particles by DC and the in vivo clearance of dying cells by macrophages. (biolegend.com)
  • Omidubicel is a stem/progenitor cell-based product composed of ex vivo expanded allogeneic cells from one entire unit of umbilical cord blood. (mycancergenome.org)
  • Proteasome inhibitor bortezomib stabilizes and activates p53 in hematopoietic stem/progenitors and double-negative T cells in vivo. (amedeo.com)
  • The objective of this initiative is to promote the thorough exploration and characterization of stem cell plasticity in hematopoietic and non-hematopoietic tissue. (nih.gov)
  • This Request for Applications (RFA), "Stem Cell Plasticity in Hematopoietic and Non-Hematopoietic Tissue" is related to one or more of the priority areas. (nih.gov)
  • Cardiogenesis requires the formation of a diverse spectrum of muscle and non-muscle cell lineages in specific tissue compartments of the heart. (nih.gov)
  • Ensuing specialists later determined that this multifunctional organ contained a number of valuable resident cells, other worthy non-indigenous cellular populations and a quantity of distinct structural elements, which possessed the potential to optimize fat graft survival and eventually further tissue engineering applications in regenerative medicine. (scirp.org)
  • These tumors are made up of fibrous tissue and usually contain cells known to cause inflammation (inflammatory cells). (nih.gov)
  • Pathologies, as atherosclerosis, cardiac fibrosis, and cardiomyopathy, are often linked to the failure of cardiovascular tissue cells to reenter the cell cycle, namely, senescence, due to endogenous or exogenous causes. (hindawi.com)
  • The workshop panel was composed of a diverse group of experts from auditory and vestibular systems as well as tissue engineers using aspects of stem cell biology in other reparative systems. (nih.gov)
  • iPSC are characterized by the ability to differentiate into numerous different cell types, representing diverse tissue. (cellapplications.com)
  • Sathe P, Metcalf D, Vremec D, Naik SH, Langdon WY, Huntington ND, Wu L, Shortman K. Lymphoid tissue and plasmacytoid dendritic cells and macrophages do not share a common macrophage-dendritic cell-restricted progenitor. (edu.au)
  • Oncogenic mutations in these cells can cause cancers that maintain a hierarchical organization reminiscent of the tissue of origin. (nature.com)
  • Most iiDMRs that were not overlapping ERV insertions occurred at tissue-specific DMRs and it cannot be ruled out that these are driven by changes in the ratio of cell types in the tissues analysed. (researchgate.net)
  • When coupled with single cell technologies for profiling the chromatin landscape, epigenome, proteome and metabolome, and spatial tissue organisation, these scRNA-seq approaches can now collect a large volume of multi-omic data about mouse embryogenesis. (biologists.com)
  • Immature dendritic cells express CD93 and down-regulate this molecule upon maturation, suggesting a role in uptake of particles by DC. (biolegend.com)
  • Single-cell transcriptomics reveals maturation of transplanted stem cell-derived retinal pigment epithelial cells toward native state. (amedeo.com)
  • Hematopoietic Jagged1 is a fetal liver niche factor required for functional maturation and engraftment of fetal hematopoietic stem cells. (amedeo.com)
  • Transgenic mice with B cells that overexpress miR-155 develop polyclonal B-cell-type pre-leukemia, [ 30 ] while miR-155-knockout mice are unable to mount a proper T-cell or B-cell immune response and also display abnormal dendritic-cell function. (medscape.com)
  • IRF8 acts primarily in myeloid cells, macrophages, dendritic cells (DCs) and microglia. (nih.gov)
  • Natural killer cells, monocytes and plasmacytoid dendritic cells (DCs) as well as CD1c + and CD141+ myeloid DCs were all present in hu mice. (nih.gov)
  • Dendritic cells are key players of the immune system. (nih.gov)
  • In 2006 she was sponsored by the Italian government to pursue her Ph.D. in molecular medicine at the Institute for Research in Biomedicine in Bellinzona, Switzerland where she studied dendritic cell development. (nih.gov)
  • Following a postdoc with A.Rolink at the University of Basel she joined Ken Murphy's group at Washington University where she investigated transcriptional regulation of dendritic cell development. (nih.gov)
  • Rodrigues PF, Alberti-Servera L, Eremin A, Grajales-Reyes GE, Ivanek R, Tussiwand R. Distinct progenitor lineages contribute to the heterogeneity of plasmacytoid dendritic cells . (nih.gov)
  • Demystifying the development of dendritic cell subtypes, a little. (edu.au)
  • Steady-state and inflammatory dendritic-cell development. (edu.au)
  • Sca-1 stands for "Stem cells antigen-1" (official gene symbol: Ly6a). (wikipedia.org)
  • [ 26 ] Lymphocytes from miR-17-92 transgenic mice have a lower threshold of activation after antigen recognition compared with those from control mice, and exhibit enhanced proliferation and reduced cell death, [ 26 ] resulting in the accumulation of activated CD4 + T and B cells. (medscape.com)
  • To overcome this hurdle, characterization of the stem-cell niche is important for understanding the molecular mechanisms whereby stem cells differentiate or self-renew. (nature.com)
  • Other areas of high interest include the ability of stem cells from non-neuronal tissues to differentiate along neuronal and glial lineages. (nih.gov)
  • Vector integration site-based lineage tracing technology was utilized to gain insights into the stages through which hPSC differentiate into mesodermal derivatives of the HSC niche. (escholarship.org)
  • Its expression is gradually lost as lineage committed progenitors differentiate. (m-entrepreneurship.com)
  • Furthermore, when expressed in megakaryocyte/erythrocyte progenitors and common lymphoid progenitors, FIP1L1-PDGFRalpha not only inhibited differentiation toward erythroid cells, megakaryocytes, and B-lymphocytes but aberrantly developed eosinophil progenitors from megakaryocyte/erythrocyte progenitors and common lymphoid progenitors. (nih.gov)
  • [ 16 ] miR-150-knockout mice have an increased number of B-1 cells and enhanced antibody production, but no defects in other types of lymphocytes have been detected. (medscape.com)
  • Human primitive hematopoietic progenitor cells are more enriched in KITlow cells than in KIThigh cells. (bdbiosciences.com)
  • The basic cellular and molecular mechanisms underlying the production and function of blood cells. (nih.gov)
  • Extracellular Gal-3 is secreted via a non-classical pathway (because of lack of signal peptides) and can bind to the cell surface through glycosylated proteins, thereby triggering or modulating cellular responses such as mediator release or apoptosis. (nature.com)
  • It is of particular importance to characterize cellular and molecular mechanisms that lead to the capacity of adult stem cells with hematopoietic potential to express other potentials and precursor cells of adult non-hematopoietic tissues to express hematopoietic potential. (nih.gov)
  • At the molecular level, in fact, a gradual weakening of the cellular processes regulating cardiovascular homeostasis occurs in aging cells. (hindawi.com)
  • Activation of the cellular senescence genetic program prompts a series of molecular changes, mostly affecting cell cycle, extracellular matrix (ECM), secretion of growth factors, and inflammatory mediators. (hindawi.com)
  • In this article, we identify a distinct cellular mechanism operative in stem cells. (altmetric.com)
  • Workshop participants recommended the development of specific reagents, assays and animal models, and increased collaboration among stem cell biologists, developmental biologists, and diabetes investigators to achieve the goal of developing a cellular replacement therapy for diabetes. (nih.gov)
  • Tissues with high cellular turnover, such as the hematopoietic system or the intestine, depend on "professional" adult stem cells for their continuous regeneration 1 . (nature.com)
  • Yet, whether AHR modulates HSPC lineage potential and directs differentiation toward specific lineage-biased progenitors is not well understood. (nih.gov)
  • Here, we show that BL-CFC expresses the receptor tyrosine kinase, Flk1, and thus we took advantage of the BL-CFC assay, as well as fluorescent activated cell sorter (FACS) analysis for Flk1 + cells to determine quantitatively if mesoderm-inducing factors promote hematopoietic lineage development. (wustl.edu)
  • Notably, we observed great variation in multi-lineage hematopoietic reconstitution in hu mice that needs to be taken into account for the experimental design with hu mice. (nih.gov)
  • In the current study, we investigated the effectiveness of TXN in rescuing mice from a lethal dose of total body radiation (TBI) and in enhancing hematopoietic reconstitution following a lethal dose of irradiation. (biomedcentral.com)
  • Schematic presentation of hematopoietic lineages and miRNAs that modulate the development of each of these lineages, as deduced from studies of miRNA mouse models. (medscape.com)
  • The mechanisms that modulate HSPC homeostasis and lineage biasing are not fully understood. (nih.gov)
  • Our research focuses on understanding how transcription factors modulate lineage differentiation and function of immune subsets. (nih.gov)
  • [ 7 , 8 ] Mice deficient for miR-17-92 are born with lung hypoplasia, ventricular septal defects and developmental abnormalities at the pro-B to pre-B-cell transition, and die shortly after birth. (medscape.com)
  • Utilizing inducible AHR knockout mice (iAHR KO), it was discovered that acute deletion of AHR doubled the number of MPP3 cells and altered the composition of downstream lineage-committed progenitors, such as increased frequency of pregranulocyte/premonocyte committed progenitors. (nih.gov)
  • Human cell chimerism and absolute human cell count decreased between week 16 and 24 in the peripheral blood of hu mice, but were stable thereafter as assessed up to 32 weeks. (nih.gov)
  • By using conditional knock-in mice, we show that the HSC defect resulting from expression of heterozygous human JAK2V617F is both quantitative (reduced HSC numbers) and qualitative (lineage biases and reduced self-renewal per HSC). (altmetric.com)
  • Moreover, mice treated with WTC-PM developed an increased burden of mutations in hematopoietic stem and progenitor cell compartments. (cdc.gov)
  • BABL/c mice were used for the survival study and a flow cytometer was used to quantify the HSC population and cell senescence. (biomedcentral.com)
  • Stanford Medicine researchers are hopeful that a proof-of-concept treatment in mice-blood stem cell transplants-may reduce signs of Alzheimer's disease. (medicalxpress.com)
  • Lineage depletion, if necessary (if not, can skip this step and combine step 1 and 6). (researchsquare.com)
  • Genetic abnormalities associated with MDS block differentiation of hematopoietic stem and progenitor cells. (medscape.com)
  • miRNA profiling studies, ectopic expression, conditional genetic ablation and transgene overexpression have been critical in the investigation of various mouse hematopoietic lineages. (medscape.com)
  • We are now covering other aspects of haematopoiesis, including how these patterns change upon duress such as infection, and how modification of genetic regulators affects the output of single cells. (edu.au)
  • Systematic screens using genetic and molecular approaches are being carried out to identify key factors involved in hematopoietic and neuronal development of zebrafish. (uclahealth.org)
  • Genetic abnormalities that involve the PDGFRA gene cause a type of blood cell cancer called PDGFRA -associated chronic eosinophilic leukemia. (nih.gov)
  • These genetic abnormalities are somatic mutations, which are mutations acquired during a person's lifetime that are present only in certain cells. (nih.gov)
  • Background: The delivery of DNA into human cells has been the basis of advances in the understanding of gene function and the development of genetic therapies. (harvard.edu)
  • In addition, advances in computational techniques have enabled the inference of developmental lineages of differentiating cells, even without explicitly introduced genetic markers. (biologists.com)
  • We have previously established that haematopoietic progenitors with very similar phenotypes often exhibit highly heterogeneous fates. (edu.au)
  • Regionally distinct progenitor cells in the lower airway give rise to neuroendocrine and multiciliated cells in the developing human lung. (amedeo.com)
  • Since July 2005, Chien has returned to Boston as Scientific Director of the Cardiovascular Research Center at Massachusetts General Hospital and Professor of Cell Biology at Harvard Medical School in the Department of Developmental & Regenerative Biology. (nih.gov)
  • He is a member of the Harvard Stem Cell Institute, where he leads the university-wide Cardiovascular Stem Cell Biology Program. (nih.gov)
  • The cell biology of adipocytes has led to a number of promising concepts in the last century, especially in the areas of obesity and associated diseases, lipid homeostasis and endocrine functions. (scirp.org)
  • Since the specialty of Plastic Surgery with its position statement on stem cells and fat grafting is committed to advancing evidence-base preclinical and clinical studies in compliance with FDA-regulations, an updated review of cell biology provides insights to achieve these goals. (scirp.org)
  • The meeting was open to the public from 8:30 a.m. on 3 November until adjournment at 12:00 p.m. for a report on the cancer initiating cell and stem cell biology. (nih.gov)
  • The Cancer Initiating Cell and Stem Cell Biology--Drs. Robert Wiltrout, Irving L. (nih.gov)
  • The purpose of the workshop was to discuss and identify potential areas of the auditory and vestibular systems that might be good candidates for applications being used in other reparative medicine systems, one being the potential use of stem cell biology. (nih.gov)
  • Through the Consortium, individual Beta Cell Biology Programs will have access to information, resources, technologies, expertise, and reagents that are beyond the scope of any single research effort. (nih.gov)
  • FUNDS AVAILABLE The NIDDK plans to commit up to $7.25 million in FY 2001 to fund approximately five new awards for Beta Cell Biology Programs. (nih.gov)
  • The award for each Beta Cell Biology Program will be limited to $1 million in direct costs/year. (nih.gov)
  • Our laboratory takes a single cell systems biology approach to studying how haematopoietic stem cells ultimately generate all of the red and white blood cells of the immune system. (edu.au)
  • We highlight challenges in analysing and interpreting these data to complement and expand our knowledge from traditional developmental biology studies in relation to cell identity, diversity and lineage differentiation. (biologists.com)
  • Our lab studies how the cells of the immune system are formed from blood stem cells. (edu.au)
  • Our ultimate goal is to advance strategies for manipulating blood stem cells that may have future applications for stem cell therapy or immune therapy, and provide insights into cancer formation. (edu.au)
  • Stem cell therapies are already being used to treat various cancers and disorders of the blood and immune system. (medicalxpress.com)
  • While such pre-leukemic stem cells (pre-LSCs) are capable of giving rise to healthy blood and immune cells, additional mutations can cause a complete block in differentiation and thereby result in the malignant expansion of aberrant progenitor cells 8 . (nature.com)
  • You will learn more about the organization of the immune system and the cells of the innate immune system. (lecturio.com)
  • Humoral immunity is mediated through B cells (producing antibodies), whereas cell-mediated immunity involves T cells, and this response is activated when the innate immune system fails to control an infection. (lecturio.com)
  • Regenerative stem cell therapies for heart disease necessitate a deep understanding of the molecular mechanisms that govern the fates and differentiation of cardiovascular precursor cells during development. (nih.gov)
  • Dr. Shuo Lin and his colleagues use zebrafish to investigate the molecular mechanisms by which diverse and specialized cell types are developed during the vertebrate embryogenesis. (uclahealth.org)
  • Molecular commonalities and other sources of cells. (nih.gov)
  • Moreover, we sought to identify molecular defects associated with leukemogenesis by utilizing induced pluripotent stem cell (iPSC) technology to model disease progression. (nih.gov)
  • These atlases allow the dissection of developmental cell lineages and molecular changes during embryogenesis. (biologists.com)
  • Functional isolation and characterization of human hematopoietic stem cells. (bdbiosciences.com)
  • Briddell RA, Broudy VC, Bruno E, Brandt JE, Srour EF, Hoffman R. Further phenotypic characterization and isolation of human hematopoietic progenitor cells using a monoclonal antibody to the c-kit receptor. (bdbiosciences.com)
  • This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD4+ T Cell Isolation Kit (Catalog #19852). (stemcell.com)
  • Matsuo Y, Adachi T, Tsubota T, Imanishi J, Minowada J. Establishment and characterization of a novel megakaryoblastic cell line, MOLM-1, from a patient with chronic myelogenous leukemia. (bdbiosciences.com)
  • Characterization of the extracellular matrix components that control the stability or expansion of hair cells. (nih.gov)
  • Characterization of cell-cell interactions involved in stabilizing hair cell-support cell interactions. (nih.gov)
  • Despite this, a thorough characterization of these cells remains elusive and with it the ability to eradicate cancer at its source. (nature.com)
  • Somatic mutations, which lead to sporadic GISTs, are present only in the tumor cells and are not inherited. (nih.gov)
  • The consequences of activating tyrosine kinase mutations for stem and progenitor cell behavior are unclear. (altmetric.com)
  • C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor. (ox.ac.uk)
  • First responders to the World Trade Center disaster, who were exposed to particulate matter containing potential carcinogens, have a high burden of somatic mutations in blood cells, raising their risk for cancer and other diseases and highlighting the need for enhanced health screening of these individuals. (cdc.gov)
  • Though this cancer is initially responsive to the drug Vemurafenib, which specifically targets cells with a BRAF-mutation, cells with other driving mutations manage to survive the drug treatment and clonally expand, resulting in significant and potentially deathly relapse of disease. (tufts.edu)
  • Spectra and characteristics of somatic mutations induced by ionizing radiation in hematopoietic stem cells. (amedeo.com)
  • Moreover, we have analyzed ES lines carrying targeted mutations for fibroblast growth factor receptor-1 (fgfr1), a receptor for basic fibroblast growth factor (bFGF), as well as scl, a transcription factor, for their potential to generate BL-CFCs and Flk1 + cells, to further define events leading to hemangioblast development. (wustl.edu)
  • Sca-1 has a regenerative role in cardiac repair: Host cells with specific Sca-1+CD31− markers arise upon myocardial infarction, with evidence of expression of Sca-1 protein. (wikipedia.org)
  • This review will focus on the cardiovascular pathologies correlated to senescence, the effect of aging on the cardiac endogenous resources of stem cells, and the potential strategies of regenerative medicine to be applied to maintain the heart younger and healthier. (hindawi.com)
  • [ 27 ] As with miR-155 (described below), the expression of miR-150 is modulated by stimulation of B cells with IgM-specific antibodies, CpG-containing DNA and LPS. (medscape.com)
  • In this system we can identify the acquisition of particular fates by cells by including fluorescently-labeled antibodies in the culture medium. (edu.au)
  • TotalSeq™-A antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions . (biolegend.com)
  • To compare the efficacy and safety of umbilical cord blood mononuclear cells (CBMNC) and azathioprine (AZA) in the treatment of patients with steroid-dependent or -resistant ulcerative colitis. (oncotarget.com)
  • Importantly, FIP1L1-PDGFRalpha but not TEL-PDGFRbeta enhanced the development of Gr-1(+)IL-5Ralpha(+) eosinophil progenitors from c-Kit(high)Sca-1(+)Lineage(-) cells. (nih.gov)
  • 15. Eosinophil cell lines. (nih.gov)
  • Stem and progenitor cells make 'decisions' in order to generate our various tissues and organs. (edu.au)
  • Single cell RNA-sequencing (scRNA-seq) technology has matured to the point that it is possible to generate large single cell atlases of developing mouse embryos. (biologists.com)
  • 1. Mechanism for the decrease in the FIP1L1-PDGFRalpha protein level in EoL-1 cells by histone deacetylase inhibitors. (nih.gov)
  • 4. Mechanisms for the proliferation of eosinophilic leukemia cells by FIP1L1-PDGFRalpha. (nih.gov)
  • 3. Differentiation of eosinophilic leukemia EoL-1 cells into eosinophils induced by histone deacetylase inhibitors. (nih.gov)
  • 14. FIP1L1-PDGFRA in chronic eosinophilic leukemia and BCR-ABL1 in chronic myeloid leukemia affect different leukemic cells. (nih.gov)
  • 16. S100A8 and S100A9 Promote Apoptosis of Chronic Eosinophilic Leukemia Cells. (nih.gov)
  • This fusion gene causes PDGFRA -associated chronic eosinophilic leukemia, which is a type of blood cell cancer characterized by an increased number of eosinophils, a type of white blood cell involved in allergic reactions. (medlineplus.gov)
  • When the FIP1L1-PDGFRA fusion gene occurs in blood cell precursors, the growth of eosinophils (and occasionally other blood cells) is poorly controlled, leading to PDGFRA -associated chronic eosinophilic leukemia. (medlineplus.gov)
  • In humans IRF8 is downregulated in a variety of myeloid cells in leukemia patients. (nih.gov)
  • Researches regulation of stem cells in the blood in normal development, aging and leukemia transformation. (jax.org)
  • Distribution and levels of cell surface expression of CD33 and CD123 in acute myeloid leukemia. (bdbiosciences.com)
  • Myeloid lineage enhancers drive oncogene synergy in CEBPA/CSF3R mutant acute myeloid leukemia. (ox.ac.uk)
  • Acute myeloid leukemia (AML) serves as a paradigm for the study of cancer stem cells 5 . (nature.com)
  • Development of hematopoietic cells occurs in the primary lymphoid organs and requires a complex transcriptional network that promotes the progression from an uncommitted self-renewing stem cell progenitor to a mature cell. (nih.gov)