• In the present study we show the reverse phenomenon in that TNF-alpha on the same progenitor cell population in combination with stem cell factor (SCF) and interleukin-7 (IL-7) through the p55 TNF receptor can recruit additional progenitors to proliferate. (ox.ac.uk)
  • We show that transient expression of six transcription factors Run1t1, Hlf, Lmo2, Prdm5, Pbx1, and Zfp37 imparts multilineage transplantation potential onto otherwise committed lymphoid and myeloid progenitors and myeloid effector cells . (bvsalud.org)
  • Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
  • In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
  • The in vitro effect of dexamethasone on the clonal growth of haematopoietic progenitors in preterm infants was investigated. (bmj.com)
  • As a first step toward the goal of defining the effects of DP treatment on haematopoiesis of preterm infants, we designed this in vitro study to determine whether DP affects the clonal growth of erythroid or granulocyte-macrophage progenitor cells in preterm infants, and if the preterm infant's progenitors respond to DP in a different manner from those of adults. (bmj.com)
  • Additionally, edited CD34 + cells can be further cultured in conditions stimulating lineage-specific differentiation by combining StemSpan™ media with expansion supplements designed for the expansion and differentiation of progenitors to erythroid, myeloid, megakaryocyte, natural killer (NK), or T cell lineages. (stemcell.com)
  • This contrasts with the mouse, where polySia and NCAM expression are restricted to multipotent hematopoietic progenitors and cells developing along a myeloid lineage. (ca.gov)
  • With this method, we will provide the first in vivo description of the human hematopoietic tree and identify new progenitors and key molecular factors involved in human hematopoiesis. (europa.eu)
  • In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
  • Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
  • PURPOSE OF REVIEW: Hematopoietic stem cells (HSCs) and progenitors are tasked with maintaining hematopoietic homeostasis in the face of numerous insults and challenges, including infection, inflammation, and exsanguination. (qmul.ac.uk)
  • In contrast, TGF-beta 1, another bifunctional regulator of hematopoietic progenitor cell growth, completely blocked SCF plus IL-7-induced proliferation. (ox.ac.uk)
  • Finally, the interaction between CD97 and its ligand CD55 regulates T-cell activation and increases proliferation and cytokine production. (wikipedia.org)
  • Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
  • To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
  • This activation is necessary for both cell proliferation as well as glucose uptake and use. (hindawi.com)
  • FGF-2 expands murine hematopoietic stem and progenitor cells via proliferation of stromal cells, c-Kit activation, and CXCL12 down-regulation. (mpg.de)
  • IL-5 is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils. (peprotech.com)
  • The ED 50 as determined by the dose-dependent proliferation of TF-1 cells was ≤ 0.15 ng/ml, corresponding to a specific activity of ≥ 6.6 x 10 6 units/mg. (peprotech.com)
  • We have found that inflammatory signals, particularly interferon gamma, influence the proliferation and differentiation of hematopoietic stem cells during infection, thus shaping the immune response as a whole. (texaschildrens.org)
  • The TAL1/SCL transcription factor regulates cell cycle progression and proliferation in differentiating murine bone marrow monocyte precursors. (nih.gov)
  • In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
  • H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
  • The reprogrammed cells , designated induced-HSCs (iHSCs), possess clonal multilineage differentiation potential, reconstitute stem /progenitor compartments, and are serially transplantable. (bvsalud.org)
  • Interestingly, the most pronounced similarities were observed among fully differentiated effector cells (Th1 and Th2 lymphocytes and mast cells) and precursors at comparable stages of differentiation (double negative thymocytes and pro-B cells), suggesting that in addition to regulating the process of commitment to particular cellular lineages, miRNAs might have an important general role in the mechanism of cell differentiation and maintenance of cell identity. (nih.gov)
  • Our microsatellite barcoding method exploits the fact that microsatellites in various loci undergo length changes during cell division, to create a natural barcode for each cell, and reconstruct the overall differentiation tree using a phylogenetic algorithm. (europa.eu)
  • Therefore, we will provide insights into both cell lineage and the molecular mechanisms involved in differentiation. (europa.eu)
  • More generally it will impact our understanding of stem cell differentiation for which hematopoiesis is used as an exemplar. (europa.eu)
  • Aged hematopoietic stem cells (HSC) exhibit compromised reconstitution capacity and differentiation-bias towards myeloid lineage, however, the molecular mechanism behind it remains not fully understood. (haematologica.org)
  • Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)
  • We have developed and applied FACS-based high-resolution cell fractionation procedures to map the differentiation processes from HSCs. (lu.se)
  • Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
  • A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
  • Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
  • Cells previously known as endothelial progenitor cells are considered now to be rather myeloid angiogenic cells than endothelial precursors [ 4 ]. (hindawi.com)
  • Therefore, the cells we used should be considered as hematopoietic precursors of myeloid angiogenic cells and are referred as PACs. (hindawi.com)
  • In addition, CD4 has also been reported to be detectable on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. (bdbiosciences.com)
  • Murine hemogenic endothelial precursors display heterogeneous hematopoietic potential ex vivo. (qmul.ac.uk)
  • Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. (wikipedia.org)
  • Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
  • Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
  • Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
  • Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
  • These factors may regulate myeloid versus lymphoid balance with age, and can potentially mitigate the long-term deleterious effects of inflammation that lead to hematopoietic pathologies. (biorxiv.org)
  • Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)
  • One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
  • Physiologic aging in both humans and mice leads to permanent changes in LT-HSC function, such as myeloid-biased hematopoietic output ( Akunuru and Geiger, 2016 ). (biorxiv.org)
  • This is the first demonstration of polySia expression and regulation on myeloid cells, and the results in animal models suggest a role for polySia in immune regulation. (ca.gov)
  • Castration of normal male mice induces expansion of the bone marrow B cell population, an effect that can be reversed by androgen replacement. (jci.org)
  • Androgens only affected B cell development in chimeric mice with androgen-sensitive stromal cells. (jci.org)
  • Two-month-old male and female pR5 mice were infused with murine p75-SAP (Cat. (atsbio.com)
  • Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
  • Kumar V, Rakha A, Saroa R, Bagai U (2015) CD4+T Cells Expansion in P. berghei (NK-65) Infected and Immunized BALB/C Mice. (omicsonline.org)
  • Experiments with B cell deficient mice have demonstrated that B cells and antibodies are essential for complete clearance of parasites from blood of the host [2]. (omicsonline.org)
  • To identify the cells responsible for PTCy-induced B cell tolerance, we selectively depleted Treg populations that were graft or HSC derived using DEREG mice. (jci.org)
  • No significant alteration of hematopoietic homeostasis and HSC function is observed in young Pus10-/- mice, while aged Pus10-/- mice exhibit mild alteration of hematopoietic homeostasis and HSC function. (haematologica.org)
  • For this, structural and functional consequences at synaptic interfaces built up by neurons and different types of glia cells in young and old mice will be characterized using conditional transgenic systems mimicking brain inflammaging and allowing cell-type-specific Notch/NF-kB modulation. (uni-ulm.de)
  • Here, we investigated bleomycin-induced lung injury responses in young and aged mice at single-cell resolution to gain insights into the cellular and molecular contributions of aging to fibrosis. (researchgate.net)
  • Fully human monoclonal antibodies are produced using transgenic mice that contain human immunoglobulin genes or using phage display (ie, a bacteriophage-based cloning method) of immunoglobulin genes isolated from human B cells. (msdmanuals.com)
  • Hematopoietic stem cells (HSCs) sustain blood formation throughout life and are the functional units of bone marrow transplantation . (bvsalud.org)
  • Single-cell analysis revealed that iHSCs derived under optimal conditions exhibit a gene expression profile that is highly similar to endogenous HSCs. (bvsalud.org)
  • Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
  • The project will investigate the age-related functional decline of hematopoiesis and dissect the role of Cdc42 in the rejuvenation of Human Hematopoietic Stem Cells (HSCs). (uni-ulm.de)
  • It will additionally focus on, using single-cell sequencing and deep learning approaches, the transcriptional and mutational heterogeneity of HSCs upon aging. (uni-ulm.de)
  • Our research focuses on how hematopoietic stem cells (HSCs) and their immediate offspring are regulated in situations such as infections, stress, aging and leukemia. (lu.se)
  • Our goal is to understand, at cellular and molecular levels, what controls HSCs and the fate of other immature blood cells. (lu.se)
  • Tumor necrosis factor-alpha (TNF-alpha) potently enhances in vitro macrophage production from primitive murine hematopoietic progenitor cells in combination with stem cell factor and interleukin-7: novel stimulatory role of p55 TNF receptors. (ox.ac.uk)
  • Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
  • Progenitor cell function may be measured post-modification by in vitro CFU assays. (stemcell.com)
  • Hematopoietic Stem Cell and Leukaemic Stem Cell environmental stress responses (In vitro biomimicry of hypoxia, normoxia, hypothermia, hyperthermia, hypoglycemia and hyperglycemia): Effect on cellular growth, cell cycle distribution, apoptosis and cellular metabolism. (imperial.ac.uk)
  • In this study, we observed that the expression of pseudouridine (Ψ) synthase 10 is increased in aged hematopoietic stem and progenitor cells (HSPC) and enforced protein of Ψ synthase 10 (PUS10) recapitulates the phenotype of aged HSC, which is not achieved by its Ψ synthase activity. (haematologica.org)
  • Understanding the molecular regulation of hematopoietic stem and progenitor cell (HSPC) engraftment is paramount to improving transplant outcomes. (qmul.ac.uk)
  • Thus, telomerase activity and telomere maintenance are associated with the immortality of cancer cells, germ-line cells, and embryonic stem (ES) cells. (nature.com)
  • The embryonic site of definitive hematopoietic stem cell (dHSC) origination has been debated for decades. (qmul.ac.uk)
  • Hematopoietic stem and progenitor cells (HSPCs) sustain life-long hematopoiesis and are first detected in the embryo by transplantation at embryonic day 10.5 (E10.5). (qmul.ac.uk)
  • It is also highly expressed in the lung mesenchyme (Fig. 8.1), initially expressed at embryonic day (E) 9.25 and later expressed throughout murine embryogenesis [2]. (keio.ac.jp)
  • We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)
  • The ability to genetically manipulate hematopoietic stem and progenitor cells (HSPCs) in particular has significantly advanced our understanding of the mechanisms that regulate hematopoiesis and is contributing to the development of novel cellular therapies. (stemcell.com)
  • Hematopoietic stem and progenitor cells (HSPCs) are necessary for life-long blood production and replenishment of the hematopoietic system during stress. (qmul.ac.uk)
  • CD34 + cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48 h. (hindawi.com)
  • EPCs are enriched in the population coexpressing CD34, CD133, and KDR with a low or absent expression of pan-hematopoietic marker CD45 [ 1 ]. (hindawi.com)
  • 5 In this study we started with a highly enriched population of CD34+ progenitor cells 6 obtained by incubating the mononuclear fraction of blood and bone marrow with biotinylated anti-CD34 antibodies and running the CD34 labelled cells over an avidin column (Cellpro, Inc., Bothell, Washington, USA). (bmj.com)
  • This Technical Bulletin provides instructions for CRISPR-Cas9 genome editing and maintenance of human CD34 + cells, including optimized pre- and post-editing culture conditions and methods to evaluate genome editing efficiency. (stemcell.com)
  • While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
  • The ArciTect™ CRISPR-Cas9 RNP is then prepared and delivered into CD34 + cells using electroporation, and cells are plated in StemSpan™ SFEM II supplemented with StemSpan™ CD34 + Expansion Supplement post-electroporation. (stemcell.com)
  • Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
  • The blood microvascular endothelium consists of a heterogeneous population of cells with regionally distinct morphologies and transcriptional signatures in different tissues and organs. (researchgate.net)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • CCL5 is a unique chemokine with distinct stage and cell-type specificities for regulating inflammation, but how these specificities are achieved and how CCL5 modulates immune responses is not well understood. (nature.com)
  • This knowledge will allow us to test whether cells with different origins have different functions, a key question in immunology in particular during infection and inflammation, and it will help design ways of therapeutically manipulating the hematopoietic system. (europa.eu)
  • We are interested in the ways that such short term hematopoietic stem cell responses may ultimately contribute to syndromes of bone marrow dysfunction that are related to infection or inflammation, such as bone marrow suppression following tuberculosis and HIV/AIDS or acquired aplastic anemia as a result of EBV infection. (texaschildrens.org)
  • This research will lead to critical insights into the basic biology of hematopoietic stem cell function, including how inflammation may contribute to hematologic changes seen in aging and cancer. (texaschildrens.org)
  • During the process of airway inflammation, complex interactions of innate and adaptive immune cells as well as structural cells and their cytokines have many important roles. (cdc.gov)
  • Interleukin (IL)-9-producing subset called Th9 cell, Th22 cells which primarily secrete IL-22, IL-13 and tumor necrosis factor- and Th25 cells via producing IL-25 are believed to be important for initiating allergic reactions and developing airway inflammation. (cdc.gov)
  • Complementary oligonucleotides capable of hybridizing to 181 miRNAs were immobilized on a membrane and probed with radiolabeled RNA derived from low molecular weight fractions of total RNA from several different hematopoietic and neuronal cells. (nih.gov)
  • In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
  • There is growing evidence that resident progenitor cell populations exist in murine lung tissues and differentiate into a mesenchymal cell lineage [1, 2]. (keio.ac.jp)
  • Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics. (peprotech.com)
  • The global clonal complexity of the murine blood system declines throughout life and after serial transplantation. (qmul.ac.uk)
  • Myelodysplastic syndrome (MDS) in childhood encompasses a diverse group of bone marrow disorders that share a common clonal defect of stem cells and that result in ineffective hematopoiesis with dysplastic changes in the marrow. (medscape.com)
  • Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
  • Tumor necrosis factor-alpha (TNF-alpha) is a bifunctional regulator of hematopoiesis, and its cellular responses are mediated by two distinct cell surface receptors. (ox.ac.uk)
  • Bcl11b is a T-cell specific gene in hematopoiesis which begins expression during T- lineage commitment and is required for this process. (ca.gov)
  • While murine hematopoiesis has been considered as resolved science, recent results using barcoding, which allows the tracing of single cells in vivo, clearly show that the topology of the murine hematopoietic tree needs revisions. (europa.eu)
  • This book was published to fill a void in the knowledge of mouse hematopoiesis and hematological cell morphology. (cshlpress.com)
  • Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
  • Recent studies using neutralizing anti-CCL5 antibodies have suggested that the constitutive expression of CCL5 plays a regulatory role in maintaining tissue-resident memory (Trm) T cells in the human vaginal tract 9 and mouse skin 10 , suggesting that constitutive CCL5 expression in noninflamed conditions, hereafter referred to as homeostatic CCL5 expression, might be important for homeostasis of tissue-resident lymphocytes. (nature.com)
  • In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
  • Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
  • The stromal cell requirement for androgen-mediated effects was confirmed in vivo by experiments using chimeric animals created by bone marrow transplantation in which androgen receptor expression was restricted to either the stromal or lymphoid cells of the bone marrow. (jci.org)
  • Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
  • We use two model pathogens, Mycobacterium avium and lymphochoriomeningitis virus, in a murine model to study these effects in vivo. (texaschildrens.org)
  • It has been reported that bone marrow-derived hematopoietic progenitor cells (HPC) migrate to secondary locations prior to tumor cells. (acponline.org)
  • Stem cell antigen-1 (Sca-1) is a cell surface glycoprotein, initially found in murine bone marrow-derived stem cell subtypes, such as hematopoietic stem cells. (keio.ac.jp)
  • MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. (thermofisher.com)
  • In pre-active lesion, increased expression of CD55 in endothelial cells and robust CD97 expression on infiltrating leukocytes suggest a possible role of both molecules in immune cell migration through the blood-brain barrier. (wikipedia.org)
  • Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
  • Neither AT alone in control media nor conditioned media nor AT-stimulated cells affected numbers of endothelial cells in the plug or plug's vascularization. (hindawi.com)
  • The process of neovascularization can be influenced not only by the residual endothelial cells but also by the circulating cells. (hindawi.com)
  • Usage: Mouse endothelial cells (MS1) and MS1 cells transduced to express full-length human TEM1 (MS1-TEM1) were cultured in 96-well plates to 30% confluence and then incubated for 96h in the presence of 10-fold serially diluted Streptavidin-ZAP, scFv78, or scFv78-ZAP starting from 40nM down to 0.04nM. (atsbio.com)
  • In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
  • Therefore, host CCL5 can obviously work as an anticancer molecule to mount relevant immune responses against some cancer cells. (nature.com)
  • CD4 + T cells stimulate B cells to induce significant level of antimalarial antibody response and also help in the induction of CD8 + T-cell responses which help in arresting the growth of liver-stage parasites in the host. (omicsonline.org)
  • This subset of T cells play critical role in maintaining immune homeostasis and controlling excessive immune responses [ 5 ]. (omicsonline.org)
  • Natural Treg cells during primary exposure to malaria enhanced Th1 memory responses and increased disease severity during re-infection. (omicsonline.org)
  • Polysialic acid, a glycan with highly restricted expression, is found on human and murine leukocytes and modulates immune responses. (ca.gov)
  • CD97 is expressed on many types of tumors including thyroid, gastric, pancreatic, esophageal, colorectal, and oral squamous carcinomas as well as glioblastoma and glioblastoma-initiating cells. (wikipedia.org)
  • Produced by mast cells, T cells, and eosinophils, IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. (peprotech.com)
  • Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)
  • they are used to treat solid and hematopoietic tumors, inflammatory disorders, and infections. (msdmanuals.com)
  • This leads to profound changes in posttransplant B cell homeostasis, including decreased B cell activating factors, increased transitional and regulatory B cells, and decreased germinal center B cells. (jci.org)
  • These findings define the mechanisms by which PTCy restores homeostasis of the B cell lineage and reestablishes immune tolerance. (jci.org)
  • CRISPR-Cas9, an RNA-guided genome editing technology, is revolutionizing cell biology due to the ease and efficiency by which it enables genetic manipulation of mammalian cells. (stemcell.com)
  • In addition to its roles in the regulation of inflammatory diseases and the maintenance of local immune cells, CCL5 expressed by cancer cells plays diverse roles in shaping cancer microenvironments toward their own survival. (nature.com)
  • Herein we show the expression of polySia on hematopoietic progenitor cells, and demonstrate a role for this glycan in immune response using both acute inflammatory and tumor models. (ca.gov)
  • CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)
  • The regulation of telomere length and telomerase activity is a complex and dynamic process that is tightly linked to cell cycle regulation in human stem cells. (nature.com)
  • CD4 + CD25 + FoxP3 + regulatory T cells (Tregs) are also involved in the mechanism of immune regulation against Plasmodium infection. (omicsonline.org)
  • Nfix Promotes Survival of Immature Hematopoietic Cells via Regulation of c-Mpl. (qmul.ac.uk)
  • Aberrant regulation of blood cell formation underlies a wide range of hematological disorders, including leukemia. (lu.se)
  • As an outlook, we aim to develop personalized anti-aging clinical interventions that will attenuate aging-driven immune remodeling and hematopoietic disorders. (uni-ulm.de)
  • Mesenchymal cells in the lung are crucial during development, but also contribute to the pathogenesis of fibrotic disorders, including idiopathic pulmonary fibrosis (IPF), the most common and deadly form of fibrotic interstitial lung diseases. (researchgate.net)
  • Pemphigus vulgaris (PV) is a life-threate- disorders and to shed a light on the role of ning blistering skin disease in which pa- mast cells in autoimmune diseases [ 7 ]. (who.int)
  • TNF-alpha generally inhibits the growth of primitive murine hematopoietic progenitor cells (Lin-Scal+) in response to multiple cytokine combinations, and the p75 TNF receptor is essential in signaling such inhibition. (ox.ac.uk)
  • PTCy, but not cyclosporine A or rapamycin, inhibits the initial alloreactive T cell response, which restores intra-bone marrow B lymphogenesis with a concomitant vigorous increase in Tregs. (jci.org)
  • Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
  • Posttransplant cyclophosphamide (PTCy) is associated with a low incidence of chronic graft-versus-host disease (cGVHD) following hematopoietic stem cell (HSC) transplantation. (jci.org)
  • MDS is rare in childhood and may have a rapidly progressive course with an extremely poor prognosis without hematopoietic stem cell transplantation (HSCT). (medscape.com)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
  • CD97 is widely expressed on, among others, hematopoietic stem and progenitor cells, immune cells, epithelial cells, muscle cells as well as their malignant counterparts. (wikipedia.org)
  • Other studies reported that some cancer cells could repress their own CCL5 expression to hinder migration of anticancer immune cells toward cancer sites 15 , 16 . (nature.com)
  • This work provides insight into how immune cells are sustained during infection, when the demand for immune effector cells is dramatically increased. (texaschildrens.org)
  • The production of distinct blood cell types can be selectively adapted to meet an individual's acute needs, such as for instance the rapid and enhanced generation of innate immune cells following infection. (lu.se)
  • CD97 in colonic enterocytes strengthens E-cadherin-based adherens junctions to maintain lateral cell-cell contacts and regulates the localization and degradation of β-catenin through glycogen synthase kinase-3β (GSK-3β) and Akt signaling. (wikipedia.org)
  • The stromal cell products responsible for these effects of estrogens have not been identified. (jci.org)
  • Stromal cell feeder layers (e.g. (stemcell.com)
  • Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. (thermofisher.com)
  • Splicing factor deficits render hematopoietic stem and progenitor cells sensitive to STAT3 inhibition. (stjude.org)
  • Typically, rapidly proliferating tumor cells have glycolytic rates up to 200 times higher than those of their normal tissue of origin, even in the presence of oxygen [ 3 ]. (hindawi.com)
  • Oral Disinfectants Inhibit Protein-Protein Interactions Mediated by the Anti-Apoptotic Protein Bcl-x(L) and Induce Apoptosis in Human Oral Tumor Cells. (mpg.de)
  • The term "pre-metastatic niche" has been used to define a particular microenvironment suitable for tumor cell attachment and growth to which primary tumor cells metastasize. (acponline.org)
  • This method allowed us to analyze cell type-specific patterns of miRNA expression and to identify miRNAs that might be important for cell lineage specification and/or cell effector functions. (nih.gov)
  • 1 In humans, glucocorticoid receptors have been shown in many different cells, including those of the granulocyte-macrophage lineage and lympho cytes. (bmj.com)
  • To identify the mechanism that controls its distinctive T-lineage expression, we corrected the identified Bcl11b transcription start site and mapped a cell-type-specific differentially methylated region bracketing the Bcl11b promoter. (ca.gov)
  • Functional dissection of the Major Peak sequence revealed distinct subregions, in which TCF-1 sites and a conserved element were required for T lineage-specific activation and silencing in non-T cells. (ca.gov)
  • The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
  • This requirement strongly limits the number of solutions or entiation and lineage-specification, programmed cell death, and ``states'' for the system. (lu.se)
  • 2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
  • These findings demonstrate that expression of a set of defined factors is sufficient to activate the gene networks governing HSC functional identity in committed blood cells . (bvsalud.org)
  • Functional screen identifies regulators of murine hematopoietic stem cell repopulation. (qmul.ac.uk)
  • Therefore, it might be safe to say that host CCL5 plays important roles in controlling cancer development but might create either pro- or anticancer environments according to the given situation, such as the type of cells that produce CCL5 and the type of cancers. (nature.com)
  • Previous studies have shown the important roles of B cell immunity in cGVHD development. (jci.org)
  • The aim of this review is to summarize the current knowledge about the possible roles of newly identified helper T cells derived cytokines (IL-9, 17, 22, 25 and IL-33) in asthma. (cdc.gov)
  • Th9 cells were first identified as a Th2 subpopulation have many important roles. (cdc.gov)
  • The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
  • Although laboratories studying stem cells, blood, and blood-forming tissues have assimilated many new molecular diagnostic methods, the identification of cell lineages through classical light microscopic techniques is often poorly understood and practiced. (cshlpress.com)
  • Rat stem-cell leukemia gene expression increased during testis maturation. (nih.gov)
  • By studying murine transgenic leukemia models developed in our laboratory, we are currently exploring the relationships between hematopoietic progenitor cell identities and the development and progression of acute leukemia. (lu.se)
  • We first demonstrated that the immune homeostatic abnormality leading to cGVHD is characterized by an initial increase in effector T cells in the bone marrow and subsequent B and Treg cytopenia. (jci.org)
  • Similarly, destruction of neonatally abundant pluripotent stem cells would likely have a more pervasive outcome than destruction of The value of incorporating immunologic appeared more severe and/or persistent when single lineages or differentiated cells that pre- data for the toxicologic assessment of drugs, the exposure occurred perinatally when com- dominate in adults. (cdc.gov)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)
  • Depending upon the cytokines secreted by the Helper T cells, they are classified into two subpopulations: Th1 and Th2 cells. (omicsonline.org)
  • However, more recent studies have revealed the potential collaboration of other helper T cells and their cytokines in this process. (cdc.gov)
  • 2018 . DOI : 10.1016/j.cell.2018.01.028. (epfl.ch)
  • 2011 - 2018, ARC Stem Cells Australia program - blood theme. (edu.au)
  • V. Fischer, M. Haffner-Luntzer, M. Amling and A. Ignatius, "Calcium and vitamin D in bone fracture healing and post-traumatic bone turnover", Eur Cell Mater , vol. 35, pp. 365-385, 2018. (uni-ulm.de)
  • In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
  • Thus, even in stem cells, except for embryonal stem cells and cancer stem cells, telomere shortening occurs during replicative ageing, possibly at a slower rate than that in normal somatic cells. (nature.com)
  • In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
  • Originally thought to behave as supporting cells for the lung epithelium and endothelium with a singular f. (researchgate.net)
  • We tested our hypothesis in a murine Lewis Lung adenocarcinoma (LLCa) model. (acponline.org)
  • When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)
  • Our technique, which is highly detailed with respect to current versions of microsatellite barcoding, provides high dimensional characterization of the cells and will allow the inference of important properties such as new phenotypic markers and key molecular regulators. (europa.eu)
  • Little is known about molecular mecha- desmosomal glycoproteins, resulting in the nisms affecting mast cell and T lympho- loss of keratinocyte cell-cell adhesion [ 1 ]. (who.int)
  • Chronic lymphoid leukaemia and hairy cell leukaemia due to chronic exposure to benzene: Report of three cases. (cdc.gov)
  • Products listed include human and murine Dendritic Cell Cytokine Packages and Hematopoietic Stem Cell Expansion Cytokine Packages. (peprotech.com)
  • Recently, the importance of telomere maintenance in human stem cells has been highlighted by studies on dyskeratosis congenital, which is a genetic disorder in the human telomerase component. (nature.com)
  • Here we review the role of telomeres and telomerase in the function and capacity of the human stem cells. (nature.com)
  • Specifically, we found that human NK cells modulate expression of NCAM and the degree of polymerization of its polySia glycans according to activation state. (ca.gov)
  • Human and murine IL-5 is cross-species reactive. (peprotech.com)
  • Chimeric monoclonal antibodies activate antigen-presenting cells (APCs) and T cells more effectively than murine monoclonal antibodies but can still induce production of human anti-chimeric antibodies. (msdmanuals.com)
  • Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
  • T cells provide protection to infection by cytokine-mediated mechanisms or through production of antibodies. (omicsonline.org)
  • Murine monoclonal antibodies are produced by injecting a mouse with an antigen, harvesting its spleen to obtain B cells that are producing antibody specific to that antigen, fusing those cells with immortal mouse myeloma cells, growing these hybridoma cells (eg, in cell culture), and harvesting the antibody. (msdmanuals.com)
  • Our results raise the prospect that blood cell reprogramming may be a strategy for derivation of transplantable stem cells for clinical application. (bvsalud.org)
  • Each of those cell sources has specific advantages and disadvantages, and each has found particular clinical applications. (medscape.com)
  • The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
  • Accumulating evidence shows that cancer stem cells are key drivers of tumor formation, progression, and recurrence. (hindawi.com)