• Despite significant advances in understanding nephron segment patterning, many questions remain about the underlying genes and signaling pathways that orchestrate renal progenitor cell fate choices and regulate differentiation. (mdpi.com)
  • Genetic abnormalities associated with MDS block differentiation of hematopoietic stem and progenitor cells. (medscape.com)
  • Small hepatic progenitor cells (SHPCs) derived from these hepatocytes have shown mature metabolic functions as well as engraftment in immunodeficient mice. (warf.org)
  • 1) Hepatocytes generated in this system display late fetal markers (both albumin and alpha-fetoprotein, AFP) and give rise to mature and engraftable small hepatic progenitor cells (SHPCs, which express albumin but not AFP). (warf.org)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • The EBF transcription factor Collier directly promotes Drosophila blood cell progenitor maintenance independently of the niche. (sdbonline.org)
  • The maintenance of stem or progenitor cell fate relies on intrinsic factors as well as local cues from the cellular microenvironment and systemic signaling. (sdbonline.org)
  • Together, this study shows that the PSC is dispensable for blood cell progenitor maintenance and reveals the key role of the conserved transcription factor Col as an intrinsic regulator of hematopoietic progenitor fate. (sdbonline.org)
  • They also showed that cross-regulation between col and eya in muscle progenitor cells is required for specification of muscle identity, revealing a new parallel between the myogenic regulatory networks operating in Drosophila and vertebrates. (sdbonline.org)
  • Recent studies have isolated distinct cell populations from infantile hemangiomas that display properties equivalent to aberrant progenitor cells, suggesting that, in addition to malignant tumors, benign tumors may also contain a stem cell-like component. (medscape.com)
  • Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)
  • IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. (biolegend.com)
  • The inventors have developed a novel system for co-differentiation of hepatocytes and hematopoietic progenitors from human embryonic stem cells (ES cells) that mimics a late fetal liver undergoing hematopoiesis. (warf.org)
  • The horizontal transfer of extracellular RNAs carried by EVs has been shown to be able to reprogram hematopoietic progenitors ( 11 ) and to activate endothelial cells ( 12 ). (spandidos-publications.com)
  • In the lymph gland , an hematopoietic organ in Drosophila larva, a group of cells called the Posterior Signaling Centre (PSC), whose specification depends on the EBF transcription factor Collier (Col) and the HOX factor Antennapedia (Antp), has been proposed to form a niche required to maintain the pool of hematopoietic progenitors (prohemocytes). (sdbonline.org)
  • In contrast with this model, this study shows that genetic ablation of the PSC does not cause an increase in blood cell differentiation or a loss of blood cell progenitors. (sdbonline.org)
  • Collier is expressed in a core population of lymph gland progenitors and cell autonomously maintains this population. (sdbonline.org)
  • The PSC contributes to lymph gland homeostasis by regulating blood cell differentiation, rather than by maintaining core progenitors. (sdbonline.org)
  • Here, we model early human neural tube development using human embryonic stem cells cultured in a microfluidic device. (nature.com)
  • Single-cell transcriptomics revealed that rostro-caudal organization was already established at 24 h of differentiation, and that the first markers of a neural-specific transcription program emerged in the rostral cells at 48 h. (nature.com)
  • 3D reconstitution of the patterned neural tube from embryonic stem cells. (nature.com)
  • Cell-cell communication network analysis demonstrated that trophoblast-like tissues supplied WNT signaling in neural crest cells to facilitate maturation and migration. (newswise.com)
  • Putative "stemness" gene jam-B is not required for maintenance of stem cell state in embryonic, neural, or hematopoietic stem cells. (taconic.com)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality. (bu.edu)
  • Using a new p16-FDR mouse line, we show that macrophages and endothelial cells are the predominant senescent cell types in murine KRAS-driven lung tumors. (bvsalud.org)
  • Through single cell transcriptomics, we identify a population of tumor-associated macrophages that express a unique array of pro-tumorigenic SASP factors and surface proteins and are also present in normal aged lungs. (bvsalud.org)
  • Macrophages derived from blood monocytes and resident macrophages produced during embryonic development can be activated to amplify control of infection, but they can also produce substances that induce unwanted excessive inflammation. (msdmanuals.com)
  • Hematopoietic stem cells emerge from endothelial cells in blood vessels during embryogenesis through the endothelial to hematopoietic transition. (biorxiv.org)
  • Here, we used single-cell transcriptomics analysis to compare embryonic and post-natal endothelial cells to investigate the potential of adult vasculature to be reprogrammed in hematopoietic stem cells. (biorxiv.org)
  • Although transcriptional similarities have been found between embryonic and adult endothelial cells, we found some key differences in term of transcription factors expression. (biorxiv.org)
  • There is a deficit of expression of Runx1, Tal1, Lyl1 and Cbfb in adult endothelial cells compared to their embryonic counterparts. (biorxiv.org)
  • Using a combination of gene expression profiling and gene regulatory network analysis, we found that endothelial cells from the pancreas, brain, kidney and liver appear to be the most suitable targets for cellular reprogramming into hematopoietic stem cells. (biorxiv.org)
  • Usage: Mouse endothelial cells (MS1) and MS1 cells transduced to express full-length human TEM1 (MS1-TEM1) were cultured in 96-well plates to 30% confluence and then incubated for 96h in the presence of 10-fold serially diluted Streptavidin-ZAP, scFv78, or scFv78-ZAP starting from 40nM down to 0.04nM. (atsbio.com)
  • Vascular endothelial junction-associated molecule, a novel member of the immunoglobulin superfamily, is localized to intercellular boundaries of endothelial cells. (taconic.com)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • Cardiovascular function depends on patent blood vessel formation by endothelial cells (ECs). (xenbase.org)
  • EVs were instrumental in transferring selected miRNAs from HLSCs to human endothelial cells absent in the latter cells. (spandidos-publications.com)
  • The aorta-gonad-mesonephros (AGM) region is of particular interest, as it autonomously generates the first adult type hematopoietic stem cells (HSCs). (ox.ac.uk)
  • The ventral position of hematopoietic clusters closely associated with the aorta of most vertebrate embryos suggests a polarity in the specification of AGM HSCs. (ox.ac.uk)
  • Spatiotemporal analysis of stem-cell-derived embryos offers conceptual and technical advances in the field. (newswise.com)
  • Leeb, M. & Wutz, A. Derivation of haploid embryonic stem cells from mouse embryos. (nature.com)
  • Embryonic stem cells come from human embryos that are three to five days old. (healthline.com)
  • There are two types of stem cell manufacturing: embryonic stem cell production, derived from early-stage embryos, and induced pluripotent stem cell (iPSC) production, which reprograms adult cells to an embryonic-like state. (imarcgroup.com)
  • E�H) E9.5 littermate mouse embryos showing defects in Rasip1 / embryos (F) and yolk sacs (H). (I�L) Whole-mount Flk1 -lacZ beta-galactosidase staining of Rasip1+/ (I and K) and Rasip1 / (J and L) yolk sacs (I and J) and embryonic heads (K and L), showing narrow Rasip1 / blood vessels that fail to remodel. (xenbase.org)
  • Fig. 3: Single-cell transcriptomics of dorsal and ventral MiSTR tissues. (nature.com)
  • Fig. 4: Single-cell transcriptomics of early MiSTR patterning and temporal dissection of MiSTR regionalization. (nature.com)
  • Single-cell resolution spatial transcriptomics defined the yolk sac erythro-megakaryopoietic niche. (newswise.com)
  • Single-cell transcriptomics shows that during the endothelial-to-hematopoietic transition, Ncx1 −/− cells fail to. (lu.se)
  • Single-cell transcriptomics shows that during the endothelial-to-hematopoietic transition, Ncx1 −/− cells fail to undergo a glycolysis to oxidative phosphorylation metabolic switch present in wild-type cells. (lu.se)
  • Aberration occurs in a stem cell that can give rise to multiple lineages. (medscape.com)
  • This event explains the presence of multiple derangements observed in the bone marrow that involve several cell lineages. (medscape.com)
  • MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
  • At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
  • In recent years, foreign scientists have also reported several times on research regarding stem cell growth and tissue regeneration carried out during space flight missions, such as blood stem cells for astronauts with anemia. (pdnews.cn)
  • Experts said that the unique space microgravity environment may be a new way to maintain undifferentiated proliferation of stem cells, enhance the efficiency of induced differentiation, and improve the level of three-dimensional tissue construction. (pdnews.cn)
  • Since positional information plays an important role in the embryonic development of several tissue systems, we tested whether AGM HSC induction is influenced by the surrounding dorsal and ventral tissues. (ox.ac.uk)
  • Chimeric explant cultures with genetically distinguishable AGM and ventral tissues show that the increase in HSC activity is not from ventral tissue-derived HSCs, precursors or primordial germ cells (as was previously suggested). (ox.ac.uk)
  • Fig. 1: Design of the MiSTR cell culture system and establishment of a WNT signaling gradient in hESC-derived tissue. (nature.com)
  • CML offers more-than-minimally manipulated cell and tissue procedures performed aseptically in our cleanroom in accordance with GMP / GTP quality compliance requirements . (cincinnatichildrens.org)
  • This means they can potentially produce new cells for any organ or tissue. (healthline.com)
  • The goal of this work is to elucidate novel molecular mechanisms that can be therapeutically targeted to regulate cell migration in a tissue-specific fashion - to inhibit it in certain contexts (e.g., inflammatory diseases and cancer metastasis) and promote it in others (e.g., stem cell transplantation and cell-based therapies). (bu.edu)
  • Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
  • Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • Glioma-associated oncogene 1 expressing (Gli1+) cells are a major source of activated fibroblasts in multiple organs, but the links between injury, inflammation, and Gli1+ cell expansion and tissue fibrosis remain incompletely understood. (bvsalud.org)
  • Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
  • Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
  • This is not necessarily a problem for the cell or tissue because multiple copies of mtDNA are present in each cell (in oocytes, this is in the region of 100,000 copies per cell), and both wild type and mutated mtDNA can coexist, a situation known as heteroplasmy. (medscape.com)
  • In 2017, the research team used the Tianzhou-1 cargo spacecraft to conduct research on the proliferation and differentiation of embryonic stem cells on a mouse. (pdnews.cn)
  • Besides the effects of NANOG in the embryonic stages of life, ectopic expression of NANOG in the adult stem cells can restore the proliferation and differentiation potential that is lost due to organismal aging or cellular senescence. (wikipedia.org)
  • The differentiated hematopoietic precursors express mature markers that show promise for engraftment (study in progress). (warf.org)
  • The analysis revealed that hematopoietic commitment capacity (PSCs to hematopoietic precursors) is correlated with the expression level of the IGF2 gene independent of the iPSC origins. (nih.gov)
  • At last, scientists had devised a way to reprogram a person's own T cells to attack tumor cells. (ajmc.com)
  • The key to curing every single cancer, which is our goal, is to have receptors that can recognize the tumor but don't recognize the normal cells. (ajmc.com)
  • The recurrence and drug resistance of breast cancer are intractable due to the presence of breast cancer stem cells (BCSCs), which are adequate to initiate tumor formation and refractory to conventional remedies. (spandidos-publications.com)
  • For instance, RUNX2 is involved in the progression of various tumor types, such as osteosarcoma, renal cell carcinoma, gastric cancer and breast cancer ( 15 - 20 ). (spandidos-publications.com)
  • The accumulation of senescent cells in the tumor microenvironment can drive tumorigenesis in a paracrine manner through the senescence-associated secretory phenotype (SASP). (bvsalud.org)
  • Genetic or senolytic ablation of senescent cells, or macrophage depletion, result in a significant decrease in tumor burden and increased survival in KRAS-driven lung cancer models. (bvsalud.org)
  • Ventral embryonic tissues and Hedgehog proteins induce early AGM hematopoietic stem cell development. (ox.ac.uk)
  • Our explant culture results at early and late embryonic day 10 show that ventral tissues induce and increase AGM HSC activity, whereas dorsal tissues decrease it. (ox.ac.uk)
  • We established the human embryonic organoid (HEMO) from expanded potential stem cells and achieved both embryonic and extraembryonic tissues in the same organoid. (newswise.com)
  • Hematopoietic tissues eventually predominated HEMO with erythropoiesis, mekagaryopiesis, and myelopoiesis. (newswise.com)
  • Cells Tissues Organs (2011) 193 (4): 239-252. (karger.com)
  • These stem cells come from developed organs and tissues in the body. (healthline.com)
  • Research in the Hagedorn Lab utilizes a combination of genetics, genomics and advanced live cell imaging in zebrafish to understand how blood and cancer cells migrate into and out of distinct tissues and organs. (bu.edu)
  • In this study, we compared hematopoietic differentiation capacity of 35 human iPSC lines derived from four different tissues and four embryonic stem cell lines. (nih.gov)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • Stem cell therapies hold immense promise in addressing these ailments by regenerating damaged tissues and organs. (imarcgroup.com)
  • Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
  • Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)
  • This can provide more beneficial assistance for the future use of stem cell regeneration to serve human health. (pdnews.cn)
  • From the experimental results, China has successfully achieved the first hematopoietic differentiation of human embryonic stem cells under space conditions, accomplishing its first experimental goal. (pdnews.cn)
  • Single-cell level characterization of embryonic development is a major benchmark of human developmental biology. (newswise.com)
  • Here, we defined the single-cell spatiotemporal gene expression landscape of human embryonic development with stem-cell-derived organoids. (newswise.com)
  • Overall, our study advances the spatiotemporal analysis of human embryonic development in stem-cell-derived organoids. (newswise.com)
  • Derivation and differentiation of haploid human embryonic stem cells. (nature.com)
  • A novel characterisation and separation technique for pluripotent human embryonic and hematopoietic stem cells. (ukri.org)
  • Erythropoiesis from human embryonic stem cells through erythropoietin-independent AKT signaling. (ca.gov)
  • Human PSC can produce red cells in culture but the process is currently inefficient producing too few cells for practical use. (ca.gov)
  • The goal of the present study was to investigate the potential of ic-MPL dimerization to induce erythropoiesis from human embryonic stem cells (hESC) and to identify the signaling pathways activated by this strategy. (ca.gov)
  • However, in recent years, there has been controversy surrounding the way human embryonic stem cells are obtained. (healthline.com)
  • Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. (novusbio.com)
  • Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. (novusbio.com)
  • Stem cell manufacturing is a highly sophisticated and rapidly evolving field dedicated to the controlled production of stem cells, which possess the unique ability to develop into umpteen specialized cell types within the human body. (imarcgroup.com)
  • The aim of the present study was to evaluate whether Alix is involved in the packaging of miRNAs within EVs released by human liver stem‑like cells (HLSCs). (spandidos-publications.com)
  • Vesicles derived from adult human liver stem-like cells (HLSCs) have also been shown to contain ribonucleoproteins and different RNA species involved in hepatic regeneration ( 20 ). (spandidos-publications.com)
  • RD), human larynx carcinoma (Hep-2), and Buffalo green shrimp and member of the family Dicistroviridae , was monkey kidney (BGMK), the three cell lines that we used recently reported to have the ability to infect primate cells. (cdc.gov)
  • Three lines of diseases caused by human enteroviruses that belong to the primate cells FRhK-4, MA-104, and BGMK, which are high- family Picornaviridae (2-7). (cdc.gov)
  • because of its reported ability to infect cultured human and monkey cells (1). (cdc.gov)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
  • Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)
  • AKT activation upregulates the GATA-1 and FOXO3 transcriptional pathways with resulting inhibition of apoptosis, modulation of cell cycle and enhanced maturation of erythroid cells. (ca.gov)
  • Overview and choice of donor of hematopoietic stem cell transplantation. (mayoclinic.org)
  • How to perform hematopoietic stem cell transplantation. (mayoclinic.org)
  • The transplantation of these cells is one of the most common treatments to cure patient suffering of blood diseases. (biorxiv.org)
  • For pediatric patients with refractory cytopenia, certain cytogenetic abnormalities, or malignant transformation, hematopoietic stem cell transplantation (HSCT) from a matched related or unrelated donor early in the course of the disease is the treatment of choice. (medscape.com)
  • These studies reveal potentially new therapeutic targets for recovery from anemia and regeneration of blood cells for transplantation. (ca.gov)
  • The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
  • Furthermore, transplantation of Lhx2-transduced hematopoietic cells into lethally irradiated mice resulted in multi-lineage repopulation of hematopoietic cells over 4 months. (ebiotrade.com)
  • MDS is rare in childhood and may have a rapidly progressive course with an extremely poor prognosis without hematopoietic stem cell transplantation (HSCT). (medscape.com)
  • Homeobox protein NANOG (hNanog) is a transcriptional factor that helps embryonic stem cells (ESCs) maintain pluripotency by suppressing cell determination factors. (wikipedia.org)
  • Cell-specific transcriptional control of mitochondrial metabolism by TIF1? (bu.edu)
  • Further, premature blood cell differentiation when PSC specification or signaling was impaired, led to assigning the PSC a role equivalent to the vertebrate hematopoietic niche. (sdbonline.org)
  • The hemolymph was known viruses of the penaeid shrimp) has not yet been suc- diluted 1:10 with Eagle's balanced salts minimum essen- cessfully cultured in any invertebrate or vertebrate cell- tial medium (EMEM), without fetal bovine sera (FBS), and culture system. (cdc.gov)
  • 1), TSV culture or CPE has not been reported in any sisted of 30 min at 48°C for reverse transcription and 10 invertebrate or vertebrate cell line. (cdc.gov)
  • Current methods to induce erythropoiesis from PSC suffer from low yields of RBCs, most of which are immature and contain embryonic and fetal rather than adult hemoglobins. (ca.gov)
  • mesenchymal stem cells are most commonly used because they are easy to obtain and present no ethical problems. (springer.com)
  • To date, RUNX2 has been involved in diverse physiological processes, including osteogenic differentiation of mesenchymal stem/stromal cells, chondrocyte hypertrophy, immunomodulation, vascular invasion and endothelial cell migration via modulating a variety of signaling cascades (e.g. (spandidos-publications.com)
  • HLSCs do not express hematopoietic stem cell markers, whereas they express several mesenchymal markers and display multiple differentiating abilities ( 22 ). (spandidos-publications.com)
  • Notch receptors and Notch ligands were detected in virtually all cells throughout EB development. (karger.com)
  • The activity of the metabolism and of growth signals contributes decisively to the development of stem cell function. (idw-online.de)
  • Whether the metabolic and dividing activity of hematopoietic stem cells during embryonic development or in adolescence already predetermines later aging of the cells had not been previously been reported and was therefore the subject of the current study. (idw-online.de)
  • The study results show that a certain growth and metabolic activity is necessary for the undisturbed development of our blood stem cells. (idw-online.de)
  • His work is focused on T cell receptor signaling and the development of T cell subsets. (rupress.org)
  • However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
  • Kearns Sayre Syndrome (OMIM #530000) occurs as a result of large-scale single deletions (or rearrangements) of mitochondrial DNA (mtDNA), which are usually not inherited but occur spontaneously, probably at the germ-cell level or very early in embryonic development. (medscape.com)
  • Wutz, A. Haploid mouse embryonic stem cells: rapid genetic screening and germline transmission. (nature.com)
  • Genetic modification and screening in rat using haploid embryonic stem cells. (nature.com)
  • These cells have been successfully used to treat children with blood cancers, such as leukemia, and certain genetic blood disorders. (healthline.com)
  • Additionally, innovations in genetic editing techniques, like CRISPR-Cas9, offer precise control over stem cell characteristics, further expanding their therapeutic potential. (imarcgroup.com)
  • Therefore, EVs may carry genetic information that may be shared among cells ( 11 - 14 ). (spandidos-publications.com)
  • hPSC-enriched essential genes mainly encode transcription factors and proteins related to cell-cycle and DNA-repair, revealing that a quarter of the nuclear factors are essential for normal growth. (nature.com)
  • These findings provide supporting evidence that enrichment for proteins involved in pluripotency is not restricted solely to malignant tumors as is suggested by the "stem cell theory of cancer", but additionally extends to common benign vascular tumors such as hemangiomas. (medscape.com)
  • Essentially, you can kill any cancer cell that has an antigen that is recognized by the immune cell," Hwu said. (ajmc.com)
  • Cell therapy involves cultivating or modifying immune cells outside the body before injecting them into the patient. (ajmc.com)
  • Initial analysis of Collier/Early B Cell Factor function in the lymph gland revealed the role of the Posterior Signaling Center (PSC) in mounting a dedicated cellular immune response to wasp parasitism. (sdbonline.org)
  • In the absence of the immune system, senescence could theoretically spread infinitely from one cell to another, but this contradicts experimental evidence. (bvsalud.org)
  • B cells The immune system consists of cellular components and molecular components that work together to destroy antigens. (msdmanuals.com)
  • Transcription factor induction of vascular blood stem cell niches in vivo. (bu.edu)
  • In addition, the red cells produced are of an immature type, expressing mostly embryonic rather than adult hemoglobin. (ca.gov)
  • The "stem cell theory of cancer" states that a subpopulation of cells with stem cell-like properties plays a central role in the formation, sustainment, spread, and drug resistant characteristics of malignant tumors. (medscape.com)
  • Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)
  • Previous studies have indicated that organic chemicals extracted from DEP result in induction of apoptosis 11 , increase oxidative stress 12 , and induce the production of inflammatory cells 13 - 15 through the release of pro-inflammatory molecules in vitro 15 , 16 . (ersjournals.com)
  • Hematopoietic Stem Cell and Leukaemic Stem Cell environmental stress responses (In vitro biomimicry of hypoxia, normoxia, hypothermia, hyperthermia, hypoglycemia and hyperglycemia): Effect on cellular growth, cell cycle distribution, apoptosis and cellular metabolism. (imperial.ac.uk)
  • Transduction of Lhx2 into ESC-derived mesodermal cells resulted in robust differentiation of c-Kit+/Sca-1+/Lineage- (KSL) cells in vitro. (ebiotrade.com)
  • For instance, a recent study by our group reported the facilitating effect of RUNX2 during aggressiveness and chemoresistance of TNBC cells via activating MMP1, which was significantly associated with poor prognosis ( 21 ). (spandidos-publications.com)
  • The results showed that the space microgravity environment provided favorable conditions for the 3D growth and maintenance of stemness of mouse embryonic stem cells, and stem cells cultured in space showed a better 3D growth mode than on the ground and maintained a higher level of pluripotent gene expression. (pdnews.cn)
  • Experimental evidence has shown that the level of NANOG p8 expression is elevated specially in cancer cells, which mean that NANOG p8 gene is a critical member in (CSCs) Cancer stem cells, so knocking it down could reduce the cancer malignancy. (wikipedia.org)
  • Although safety remains a concern, investigators are breaking ground in cell and gene therapy, and many believe that ultimately, a string of cancer cures will follow. (ajmc.com)
  • Although only a handful of cell and gene therapies are on the market, FDA officials predicted in 2019 that the agency will receive more than 200 investigational new drug applications per year for cell and gene therapies, and that by 2025, it expects to have accelerated to 10 to 20 cell and gene therapy approvals per year. (ajmc.com)
  • Gene therapy modifies or manipulates cell expression. (ajmc.com)
  • Juliette Hordeaux, PhD, senior director of translational research for the University of Pennsylvania's gene therapy program, is cautious about the FDA's predictions, saying she'd be "thrilled" with 5 cell and/or gene therapy approvals annually. (ajmc.com)
  • Safety has been the main brake around adeno-associated virus vector (AAV) gene therapy," added Hordeaux, whose hospital's program has the institutional memory of both Jesse Gelsinger's tragic death during a 1999 gene therapy trial as well as breakthroughs by Carl June, MD, and others in CAR T-cell therapy. (ajmc.com)
  • The Cell Manipulations Laboratory's experienced scientists can help you develop and scale up your ex-vivo gene transfer and cell therapy clinical trials or simply transfer your existing at-scale technology to our clean facilities which will enable you to move your research to a safe and effective patient treatment plan. (cincinnatichildrens.org)
  • Researchers and clinicians can contact us for more information about how we can help you with your cell therapy and gene transfer needs. (cincinnatichildrens.org)
  • Researchers at the Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) have now found a gene mechanism that is responsible for the aging of hematopoietic stem cells. (idw-online.de)
  • The gene Igf2bp2 is important in youth for the full function of these cells, as it activates their growth and metabolism. (idw-online.de)
  • When the gene is missing, however, the aging-associated loss of function of the stem cells is surprisingly diminished. (idw-online.de)
  • The eventual aging of hematopoietic stem cells is apparently already preprogrammed by their gene-driven growth in youth. (idw-online.de)
  • After that, the gene is silenced and loses its function, it shows hardly any activity in the stem cells in advanced age," explains Prof. K. Lenhard Rudolph, research group leader at the FLI and professor of molecular medicine at FSU Jena. (idw-online.de)
  • Surprisingly, mice in which the gene is mutated show a reduction in the age-associated loss of function of the blood stem cells in late life, even though the gene is no longer active. (idw-online.de)
  • This suggests that Igf2bp2 gene function in early life leads to the aging of the stem cells. (idw-online.de)
  • The Igf2bp2-gene drives growth and metabolic activity at a young age but these activities contribute to the age-associated loss of hematopoietic stem cell function in later life. (idw-online.de)
  • Induced pluripotent stem (iPS) cells have great potential for regenerative medicine and gene therapy. (stemcell.com)
  • IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. (biolegend.com)
  • In this paper we found that if we introduced a synthetic "fusion" protein into PSC, we could produce significantly more red cells in culture and that those cells were also more mature. (ca.gov)
  • We also found that the synthetic protein acts to increase red cell production independently of the well known growth factor erythropoietin. (ca.gov)
  • Experiments revealed that the fusion protein activates unusual signaling pathways during PSC differentiation leading to changes in cycling and survival of the red cells. (ca.gov)
  • Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. (novusbio.com)
  • Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). (novusbio.com)
  • However, these cells reside in an abnormal microenvironment, fail to activate the hematopoietic program downstream of Runx1, and are functionally impaired. (lu.se)
  • Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells. (nature.com)
  • Androgenetic haploid embryonic stem cells produce live transgenic mice. (nature.com)
  • Researchers at the Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) in Jena have been able to demonstrate that in mice, the growth factor Igf2bp2 controls hematopoietic stem cell function in young adulthood by activating stem cell metabolism and growth. (idw-online.de)
  • The experimental findings of the current study suggest that the activation of growth and metabolism in juvenile mice preprograms the subsequent loss of function of hematopoietic stem cells and inscribes this into the cell's memory. (idw-online.de)
  • Using this expertise, the scientists were able to identify a new subset of hematopoietic stem cells that exhibit particularly strong activity of Igf2bp2-dependent metabolism and growth in adolescent mice. (idw-online.de)
  • Fig. 2: Analysis of cell-essential genes. (nature.com)
  • The research group specializes in the study of genes at the single-cell level. (idw-online.de)
  • Protection from UV light is an evolutionarily conserved feature of the haematopoietic niche. (bu.edu)
  • Distinct Roles for Matrix Metalloproteinases 2 and 9 in Embryonic Hematopoietic Stem Cell Emergence, Migration, and Niche Colonization. (bu.edu)
  • Stem cell niche. (bvsalud.org)
  • American Journal of Respiratory Cell and Molecular Biology. (lu.se)
  • Forward and reverse genetics through derivation of haploid mouse embryonic stem cells. (nature.com)
  • Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. (novusbio.com)
  • DNMT3A and TET1 cooperate to regulate promoter epigenetic landscapes in mouse embryonic stem cells. (stembook.org)
  • Here, we show that HSC-like cells were efficiently induced from mouse ESCs by enforced expression of Lhx2, a LIM-homeobox transcription factor. (ebiotrade.com)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
  • These data demonstrated that variations in the hematopoietic differentiation capacity of iPSCs are not attributable to somatic memories of their origins. (nih.gov)
  • Bisulfite converted genomic DNA lysates from induced pluripotent stem cell and embryonic stem cell lines were hybridized to Illumina HumanMethylation450 BeadChip. (nih.gov)
  • Q and R) Ventral views of E8.5 embryonic paired dorsal aortae. (xenbase.org)
  • Pulmonary exposure to NQ dose-dependently aggravated antigen-related airway inflammation, as characterised by infiltration of eosinophils and lymphocytes around the airways and an increase in goblet cells in the bronchial epithelium. (ersjournals.com)
  • NANOG is a transcription factor in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. (wikipedia.org)
  • In this embryonic stage, NANOG represses Pou3f1, a transcription factor crucial for the anterior-posterior axis formation. (wikipedia.org)
  • Nanog is a transcription factor that controls both self-renewal and pluripotency of embryonic stem cells. (wikipedia.org)