To investigate the developmental processes that regulate mesoderm induction and specification to the hemangioblast, we generated an embryonic stem cell line with the green fluorescent protein (GFP) targeted to the mesodermal gene, brachyury. (elsevierpure.com)
Ligand-dependent development of the en-dothelial and hemopoietic lineages from embryonic mesodermal cells expressing vascular endothelial growth factor receptor 2. (chinagene.cn)
2 It is thought that ECs originate from mesoderm-derived angioblasts and hemangioblasts during embryonic and adult development. (beckman.com)
During embryonic development mesodermal differentiate into endothelial cells, hemangioblast and hematopoietic progenitors give rise to blood vessel, the vasculogenesis. (samploon.com)
Progenitor3
The hematopoietic and endothelial lineages derive from mesoderm and are thought to develop through the maturation of a common progenitor, the hemangioblast. (elsevierpure.com)
Angioblast, the endothelial progenitor, is generated from hemangioblasts that are derived from the Flk1+ mesodermal cells, or directly from the Flk1+ mesodermal cells. (chinagene.cn)
The hemangioblast theory, which posits that the RBCs and ECs derive from a common progenitor cell, was developed as researchers observed that receptor knockout mice, such as Flk1-/-, exhibited defective RBC formation and vessel growth. (wikipedia.org)
Angioblasts2
Vessel development involves generating primary vascular endothelial tubes by aggregation of angioblasts (vasculogenesis), creating a vascular network through endothelial sprouting (angiogenesis), and pruning primary vascular tubes by recruiting smooth muscle cells to the vessel walls (vessel maturation). (chinagene.cn)
Although several factors such as vegf, flk1, cloche, lycat and estrp are essential for angioblast development, much of the signaling pathways underlying the derivation of angioblasts from the hemangioblasts or Flk1+ mesodermal cells remain unknown. (chinagene.cn)
Vessel1
Our data suggest that zebrafish Runx1 functions in both blood and vessel development at the hemangioblast level, and contributes to both primitive and definitive hematopoiesis. (biologists.com)
Cell3
Rat stem-cell leukemia gene expression increased during testis maturation. (nih.gov)
Co-expression of GFP with the receptor tyrosine kinase Flk1 revealed the emergence of three distinct cell populations, GFP - Flk1 - , GFP + Flk1 - and GFP + Flk1 + cells, which represent a developmental progression ranging from pre-mesoderm to prehemangioblast mesoderm to the hemangioblast. (elsevierpure.com)
However, Ueno and Weissman provided the earliest contradiction to the hemangioblast theory when they saw that distinct ES cells mixed into a blastocyst resulted in more than 1 ES cell contributing to the majority of the blood islands found in the resultant embryo. (wikipedia.org)
iPSC and hESC-derived hemangioblast formed mixed type of hematopoietic colony. (1library.org)
We have previously shown that endothelial cells of the aortic floor give rise to hematopoietic cells, revealing the existence of an aortic hemangioblast. (silverchair.com)
Differentiation2
19. Genome-wide analysis of the zebrafish ETS family identifies three genes required for hemangioblast differentiation or angiogenesis. (nih.gov)
Importantly, this approach significantly increased production of human HSPCs with self-renewal and multilineage differentiation properties, as well as phenotypic and molecular evidence of progressive maturation in culture. (nih.gov)
Indicated the existence1
Other studies done in zebrafish have more soundly indicated the existence of the hemangioblast. (wikipedia.org)
Cells3
At this stage of maturation, mononuclear cells are present with parallel arrays of myofibrillar bundles, with a sarcomeric organization. (medscape.com)
out hemangioblast formation that produced CD71+HbF+erythroid cells. (1library.org)
However, Ueno and Weissman provided the earliest contradiction to the hemangioblast theory when they saw that distinct ES cells mixed into a blastocyst resulted in more than 1 ES cell contributing to the majority of the blood islands found in the resultant embryo. (wikipedia.org)