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  • Proteins
  • Although here we focus on influenza virus surface glycoproteins, this method can also be used to produce other viral and cellular surface proteins. (jove.com)
  • MDCK cells (10 4 or 5 x 10 3 per well) were cultured in 96- or 384-well plates, infected with PR8 and viral proteins were detected using anti-M2 followed by an IR dye-conjugated secondary antibody. (jove.com)
  • infectious
  • Therapeutic strategies targeting regulation of NK cell receptor and ligand system have shown great promise for treatment of cancer and viral infectious diseases. (els.net)
  • HCV RNA transfected cells released infectious particles into culture supernatant and the viral titer was measured. (jove.com)
  • Consequently, successful trans -complementation, which is determined by measuring de novo production of infectious viral progeny, indicates absence of dominant restrictions. (jove.com)
  • immune
  • Extensive research has been performed to understand the molecular viral mechanisms involved in the H5N1 pathogenesis in humans, providing interesting insights about the virus-host interaction and the regulation of the innate immune response by these highly pathogenic viruses. (mdpi.com)
  • humans
  • Interestingly, these H5 viruses, with increased affinity to α2,6 SA, emerged during viral diversification in bird populations and subsequently spread to humans. (nih.gov)
  • cells
  • Scanning of PR8-infected MDCK cells in the IR scanner indicated a viral titer-dependent bright fluorescence. (jove.com)