• The manifestation levels in control cells were arranged to 1 1. (synanet2020.com)
  • EpH4-Cl3 cells were incubated with DMSO (Control) or 1 M GSK for 120 min. (synanet2020.com)
  • When cells were transfected with GFP-HIF-1α, treatment with TSA increased the number of green fluorescence protein (GFP)-positive cells. (oncotarget.com)
  • GATA-1 forms distinct activating and repressive complexes in erythroid cells. (ox.ac.uk)
  • Using a biotinylation tagging/proteomics approach in erythroid cells, we describe distinct GATA-1 interactions with the essential hematopoietic factor Gfi-1b, the repressive MeCP1 complex and the chromatin remodeling ACF/WCRF complex, in addition to the known GATA-1/FOG-1 and GATA-1/TAL-1 complexes. (ox.ac.uk)
  • Impact of histone deacetylase inhibitor valproic acid on the anticancer effect of etoposide on neuroblastoma cells. (nel.edu)
  • The effects of etoposide and VPA on UKF-NB-4 cells were tested under the normoxic and also the hypoxic (1% O2) cultivation conditions. (nel.edu)
  • Groh T, Hrabeta J, Poljakova J, Eckschlager T, Stiborova M. Impact of histone deacetylase inhibitor valproic acid on the anticancer effect of etoposide on neuroblastoma cells. (nel.edu)
  • Anti-proliferative activity and apoptotic effect of tick salivary gland extracts on human HeLa cells. (nel.edu)
  • The anti-proliferative and apoptotic potential of salivary gland extracts (SGE) of unfed and partially fed adult ixodid ticks were screened using human HeLa cells. (nel.edu)
  • Kazimírová M, Dovinová I, Rolníková T, Tóthová L, Hunáková L. Anti-proliferative activity and apoptotic effect of tick salivary gland extracts on human HeLa cells. (nel.edu)
  • MC1568 is a member of HDAC inhibitors that inhibits the histone deacetylase class II by modifying the genetic expression of various important genes of cell cycle mostly at G1 phase, resulting in the death of breast cancer cells by apoptosis. (adooq.com)
  • Pyroxamide (NSC 696085) is a potent inhibitor of affinity-purified HDAC1 and causes the accumulation of acetylated core histones in MEL cells cultured with the agent. (adooq.com)
  • Given that we have an efficiency of 1% cloning for livestock species and if only one in a thousand cells are viable then around 100,000 cells would need to be transferred. (wikiquote.org)
  • The ability of E2F-1 to prompt apoptosis in DNA damaged cells coincides with enhanced PRMT1 methylation. (embl.de)
  • Similarly, the recruitment of protein acetyltransferases positively correlates with histone hyperacetylation at active genes as shown by genome-wide chromatin immunoprecipitation (ChIP-Seq) studies of CBP and p300 in human T cells [ 3 ]. (aging-us.com)
  • Men and women wild-type (WT) and also Fat-1 mice have been implemented dextran sea salt sulfate (DSS) within the mineral water (2.5%) in order to stimulate intense (Five n DSS) or long-term (3 series DSS) colitis and also the percentage of Treg along with Th17 cells existing locally [colonic lamina propria (cLP) as well as systemically (spleen) was resolute through movement cytometry. (atpase-signal.com)
  • Recent high-impact research has shown the genomic/genetic differences between cancer and normal cells using methods such as next-generation sequencing [ 1 , 2 ]. (intechopen.com)
  • TSA acetylated HIF-1α at lysine (K) 674, which led to an increase in TSA-induced VEGF-HRE reporter activity. (oncotarget.com)
  • Positive charges on histone tails are attracted to the negative DNA backbone, but this is neutralized by acetylation of the lysine residues. (biologists.com)
  • Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each. (lookformedical.com)
  • Protein lysine acetyltransferases (HATs or PATs) acetylate histones and other proteins, and are principally modeled as transcriptional coactivators. (aging-us.com)
  • In this study, we investigated the effect of this anticancer drug alone and in combination with a histone deacetylase (HDAC) inhibitor, valproic acid (VPA), on a human UKF-NB-4 neuroblastoma cell line. (nel.edu)
  • Innovative DNA-Targeted Metallo-prodrug Strategy Combining Histone Deacetylase Inhibition with Oxidative Stress, T. McGivern et al. (baseclick.eu)
  • Finally, increasing microtubule stability through pharmacological inhibition of histone deacetylase 6 (HDAC6) rescues defects in the intracellular distribution of mitochondria and BAX. (en-journal.org)
  • Analysis of CBP and p300 mutant mouse fibroblasts reveals CBP/p300 are together chiefly responsible for the global acetylation of histone H3 residues K18 and K27, and contribute to other locus-specific histone acetylation events. (aging-us.com)
  • Features such as the transcriptome, methylome, proteome, histone post-translational modifications and the microbiome all influence the host response to various diseases and cancers. (rsc.org)
  • In this context, numerous histone post-translational modifications (PTMs) have been described that include acetylation, phosphorylation, methylation, ubiquitylation and SUMOylation [ 1 ]. (aging-us.com)
  • An increase in the interaction between HIF-1α and the VEGF promoter, which was assessed by a chromatin immunoprecipitation (ChIP) assay, led to activation of the VEGF promoter. (oncotarget.com)
  • SMC3 is acetylated during S-phase to establish cohesiveness of chromatin-loaded cohesin, and in yeast, the class I histone deacetylase Hos1 deacetylates SMC3 during anaphase. (nih.gov)
  • Chromatin assembly factor 1. (lookformedical.com)
  • A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. (lookformedical.com)
  • Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. (embl.de)
  • The canonical nucleosome is a fundamental unit of chromatin and consists of a protein octamer of two molecules each of histone H2A, H2B, H3 and H4, wrapped by 147 bp of genomic DNA. (aging-us.com)
  • In the present study, we investigated whether TSA treatment increases HIF-1α stabilization via acetylation under normoxic conditions, which would lead to VEGF upregulation and resistance to anticancer drugs. (oncotarget.com)
  • These data demonstrate that HIF-1α may be stabilized and translocated into the nucleus for the activation of VEGF promoter by TSA-mediated acetylation at K674 under normoxic conditions. (oncotarget.com)
  • These findings suggest that HIF-1α acetylation may lead to resistance to anticancer therapeutics, such as HDAC inhibitors, including TSA. (oncotarget.com)
  • Is histone acetylation the most important physiological function for CBP and p300? (aging-us.com)
  • Although studies in yeast show that many histone mutations cause modest or specific phenotypes, similar studies are impractical in mammals and it remains uncertain if histone acetylation is the primary physiological function for CBP/p300. (aging-us.com)
  • CBP/p300 can also be important for transcription, but the recruitment of CBP/p300 and their associated histone acetylation marks do not absolutely correlate with a requirement for gene activation. (aging-us.com)
  • Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. (ebiocell.com)
  • Belinostat (PXD101) is a HDAC inhibitor that inhibits HDAC activity in HeLa cell extracts with an IC50 of 27 nM. (adooq.com)
  • In addition, TSA-mediated cell death was reduced by the overexpression of HIF-1α but it was rescued by transfection with a HIF-1α mutant (K674R). (oncotarget.com)
  • We also show that subsets of GATA-1 gene targets are bound in vivo by distinct complexes, thus linking specific GATA-1 partners to distinct aspects of its functions. (ox.ac.uk)
  • c-Myc transrepression is mediated by blockade of the transactivating activity of Miz-1, which occurs mainly through a Sp1-binding site in the proximal promoter of the gene. (aacrjournals.org)
  • Histone PTMs often correlate with the activity of the gene (or of a regulatory element such as an enhancer) that is in their proximity, suggesting a causal relationship. (aging-us.com)
  • Exceptions to this limitation occur when analyzing histone variants that have few gene copies, or when assessing putative gain-of function histone mutations, such as those identified in pediatric glioblastoma and glioma (e.g. (aging-us.com)
  • The difficulty of testing histone point mutations in mammals has therefore contributed to the uncertainty of whether canonical histone PTMs are correlative with gene expression or causal. (aging-us.com)
  • Phenotype and gene expression changes are often surprisingly moderate or specific in yeast that harbor point mutations in the N-terminal tails of histones H2A, H2B, H3 and H4 [ 9 , 16 - 19 ]. (aging-us.com)
  • A histone chaperone that facilitates nucleosome assembly by mediating the formation of the histone octamer and its transfer to DNA. (lookformedical.com)
  • Arsenic exposure activated ferritin transcription via the ARE concomitant with increased methylation of histones H4Arg3 (H4R3) and H3Arg17 (H3R17). (embl.de)
  • To test our hypothesis that histone H4R3 and H3R17 methylation regulates ferritin transcription, H4R3 and H3R17 protein arginine (R) methyltransferases 1 and 4 (PRMT1 and PRMT4) were investigated. (embl.de)
  • Arsenic exposure of human HaCaT keratinocytes induced nuclear accumulation of PRMT1 and PRMT4, histone H4R3 and H3R17 methylation proximal to the ARE, but not to the non-ARE regions of ferritin genes. (embl.de)
  • Arginine methylation-dependent reader-writer interplay governs growth control by E2F-1. (embl.de)
  • We describe the residue-specific methylation of E2F-1 by the asymmetric dimethylating protein arginine methyltransferase 1 (PRMT1) and symmetric dimethylating PRMT5 and relate the marks to different functional consequences of E2F-1 activity. (embl.de)
  • Methylation by PRMT1 hinders methylation by PRMT5, which augments E2F-1-dependent apoptosis, whereas PRMT5-dependent methylation favors proliferation by antagonizing methylation by PRMT1. (embl.de)
  • In contrast, cyclin A binding to E2F-1 impedes PRMT1 methylation and augments PRMT5 methylation, thus ensuring that E2F-1 is locked into its cell-cycle progression mode. (embl.de)
  • The Tudor domain protein p100-TSN reads the symmetric methylation mark, and binding of p100-TSN downregulates E2F-1 apoptotic activity. (embl.de)
  • Hypoxia-inducible factor 1 (HIF-1) participates in tumor angiogenesis by upregulating target genes, such as vascular endothelial growth factor (VEGF). (oncotarget.com)
  • The function of histone PTMs in mammals remains uncertain because the multiple genes encoding each canonical histone, renders in vivo mutational analysis unfeasible in most instances. (aging-us.com)
  • LAQ824 (NVP-LAQ824) is a potent novel histone deacetylase inhibitor with significant activity against multiple myeloma. (adooq.com)
  • Here we identify HDAC8 as the vertebrate SMC3 deacetylase, as well as loss-of-function HDAC8 mutations in six CdLS probands. (nih.gov)
  • MGCD0103 (Mocetinostat) is a benzamide histone deacetylase inhibitor by inhibiting mainly histone deacetylase 1 (HDAC1), but also HDAC2, HDAC3, and HDAC11. (adooq.com)
  • 1) self-sufficiency in growth signals, 2) insensitivity to antigrowth signals, 3) evasion of apoptosis, 4) limitless replicative potential, 5) sustained angiogenesis, and 6) tissue invasion/metastasis. (theinterstellarplan.com)
  • Relevant to the role of CBP/p300 as HATs, hyperacetylation of histone N-terminal tail lysines correlates strongly with active transcription [ 2 ]. (aging-us.com)
  • In baker's yeast, however, histone mutations can be easily made and numerous studies show that histone PTMs might not be as essential as their correlative behavior with transcription would suggest [ 9 - 14 ]. (aging-us.com)
  • Sirtuin 1/SIRT1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Sirtuin 1/SIRT1 knockout HeLa cell line. (rndsystems.com)
  • Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SIRT1 knockout HeLa cell line (KO). (rndsystems.com)
  • The retroelement long interspersed element-1 ( LINE-1 or L1) comprises about 17% of the human genome. (researchsquare.com)
  • For instance, a systematic analysis of 486 different histone H3 and H4 mutations in yeast (where every residue was mutated at least one way) showed that only 11 of 79 N-terminal tail deletions resulted in lethality, a phenotype that also depends to some extent on strain background [ 9 ]. (aging-us.com)
  • TSA also enhanced the nuclear translocation of HIF-1α protein, as assessed by immunoblotting and as evidenced by increased nuclear localization of GFP-HIF-1α. (oncotarget.com)
  • Cell lines such as E. coli, HeLa, HEK, MOLM are arguably among the most routine applications, but also animals, like mouse, rat, the nematode C. elegans, crickets (Gryllus bimaculatus), chicken (Gallus domesticus) and zebra fish (Danio rerio) or even plants (e.g. (baseclick.eu)
  • 1 Division of Human Genetics and Molecular Biology, The Children's Hospital of Philadelphia, Pennsylvania 19104, USA. (nih.gov)
  • CVF also facilitates the confinement of exogenous organisms (summarized in Figure 1 ) ( 1 - 3 ). (frontiersin.org)
  • PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Sirtuin 1/SIRT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7714) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). (rndsystems.com)
  • HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human Sirtuin 1/SIRT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7714, filled histogram) or isotype control antibody (Catalog # 5-001-A , open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127 ). (rndsystems.com)
  • Based on these findings, we suggest a model for the different roles of GATA-1 in erythroid differentiation. (ox.ac.uk)
  • TSA enhanced total HIF-1α and VEGF-HRE reporter activity under normoxic conditions. (oncotarget.com)
  • The mechanisms that underlie and dictate the different biological outcomes of E2F-1 activity have yet to be elucidated. (embl.de)
  • Our results define an exquisite level of precision in the reader-writer interplay that governs the biological outcome of E2F-1 activity. (embl.de)
  • Detection of Human Sirtuin 1/SIRT1 by Western Blot. (rndsystems.com)
  • Western Blot Shows Human Sirtuin 1/SIRT1 Specificity by Using Knockout Cell Line. (rndsystems.com)
  • Western blot analysis of whole cell lysates probed with Mouse anti Human proliferation associated protein 2G4 antibody ( VMA00111 ) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P ) and visualized on the ChemiDoc MP with 28 second exposure. (bio-rad-antibodies.com)