• In vitro characterization and delivery of chitosan-DNA microparticles into mammalian cells. (ualberta.ca)
  • In the present study, we have investigated DNA encapsulation efficiency and loading capacity of chitosan microparticles prepared in different conditions, as well as in vitro DNA release from microparticles, and transfection of different cell lines with chitosan-DNA microparticles, which may be employed in future in vivo studies. (ualberta.ca)
  • Although the transfection efficiency of chitosan-DNA microparticles was typically lower than that of DNA complexed with lipid-based reagents, in vitro transfection results indicated that HEK293 cells take up chitosan-DNA microparticles more efficiently compared to HeLa and mouse fibroblastic 3T3 cell lines. (ualberta.ca)
  • The efficient delivery of functional therapeutic genes into target cells in vitro and in vivo is an important problem in gene therapy approaches for the treatment of hereditary diseases and cancer (9, 10). (ualberta.ca)
  • Chitosan microparticles containing reporter genes are being used for the transfection of mammalian cells both in vitro and in vivo conditions (28-30). (ualberta.ca)
  • The application of hydrogels as a matrix for 3-dimensional cell cultures has become an indispensable tool in tissue engineering, biotechnology and biomedical research due to the improved functionality and viability of the in vitro biological system. (heraldopenaccess.us)
  • 2000. Results of the L5178Y mouse lymphoma assay and the Balb/3T3 cell in vitro transformation assay for eight phthalate esters. (cdc.gov)
  • Furthermore, MsV can reduce in vitro cell viability in MGSO-3 cell line derived from human breast cancer tissue. (bvsalud.org)
  • HeLa (human cervical carcinoma), DLD-1 (human colon adenocarcinoma) and Balb/c 3T3 (Swiss mouse embryo) cell lines (purchased from ATCC, Manassas, VA) and HUVEC (human umbilical vein endothelial cells, bought from Cambrex, Walkersville, VA) had been also employed. (estrogen-receptor.com)
  • Western blot analysis of extracts of various cell lines, using GAPDH Monoclonal Antibody (AC033) at 1:20000 dilution. (aipunuobio.com)
  • Western blot analysis of extracts of HeLa cells, using GAPDH antibody (AC033) at 1:10000-1:2560000 dilution. (aipunuobio.com)
  • 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody and ARG55799 anti-LC3B antibody at 1:1000 dilution. (arigobio.com)
  • Cells were stained with ARG55799 anti-LC3B antibody at 1:100 dilution. (arigobio.com)
  • Immunofluorescence analysis of C6 cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). (aipunuobio.com)
  • Immunofluorescence analysis of NIH-3T3 cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). (aipunuobio.com)
  • Immunofluorescence: NIH/3T3 cells treated with Chloroquine (50 μM, 37°C for 20 hours). (arigobio.com)
  • Swiss 3T3 (mouse), A431 (human) and HeLa (human) cells were serum starved followed by stimulation by LPA. (cytoskeleton.com)
  • The different isozymes -100, 69, 46 and 40 kDa expressed in human cells, or the 105, 71 and 43 kDa expressed in mouse cells--are induced by IFNs with cell type specificity, and exhibit individual differences in their biochemical and enzymatic properties. (elsevierpure.com)
  • Moreover, using a mouse Swiss 3T3 cell line in which the cDNA coding for PKC-alpha was introduced, leading to its overexpression, we could show that the mouse 105 kDa synthetase was constitutively expressed. (elsevierpure.com)
  • Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. (aipunuobio.com)
  • This G-LISA™ Rho activation assay measures the levels of GTP-loaded RhoA in cells. (cytoskeleton.com)
  • However the autocrine IFN in the medium of the cells overexpressing PKC is not able to induce 2-5 A synthetase in control transfected Swiss 3T3 cells. (elsevierpure.com)
  • These potent effects are driven by curcumin's ability to induce G2/M cell cycle arrest, induce autophagy, activate apoptosis, disrupt molecular signaling, inhibit invasion and metastasis, and increase the efficacy of current chemotherapeutics. (hindawi.com)
  • The life cycle of retroviruses is arbitrarily divided into two distinct phases: the early phase refers to the steps of infection from cell binding to the integration of the viral cDNA into the cell genome, whereas the late phase begins with the expression of viral genes and continues through to the release and maturation of progeny virions (see Figure 1 for a schematic view of the retroviral life cycle). (biomedcentral.com)
  • In some experiments, the conditioned medium was replaced with fresh medium just after 24 hours of stimulation and the cells were then maintained in culture for up to 48 hours. (estrogen-receptor.com)
  • IκBζ, an atypical member of the nuclear IκB family of proteins, is expressed at low levels in most resting cells, but is induced upon stimulation of Toll-like/IL-1 receptors through an IRAK1/IRAK4/NFκB-dependent pathway. (oncotarget.com)
  • The G-LISA Rho activation assays are ELISA based Rho activation assays with wich you can measure Rho activity in cells in less than 3 h. (cytoskeleton.com)
  • Soon after growing in cultures for the proper instances in unique conditions, the cells have been either lysed for total RNA extraction or utilized for functional assays. (estrogen-receptor.com)
  • More recent research applications include microchips for genomics, proteomics and cell-based assays. (heraldopenaccess.us)
  • Cell viability, colony formation, and wound healing assays were performed to determine proliferative and migratory characteristics. (fabad.org.tr)
  • Data shown are relative luminescence units (RLU) over background signal (wells incubated with lysis buffer alone instead of cell lysates). (cytoskeleton.com)
  • Transfection efficiency of chitosan-DNA microparticles into mammalian cells was determined by measuring the b -galactosidase activity in cell lysates. (ualberta.ca)
  • Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines. (hsanmartino.it)
  • Long studied as a key component of the immune response, IκBζ emerges as an important regulator of inflammation, cell proliferation and survival. (oncotarget.com)
  • Low power laser irradiation (LPLI) promotes proliferation of multiple cells, which (especially red and near infrared light) is mainly through the activation of mitochondrial respiratory chain and the initiation of cellular signaling. (biomedcentral.com)
  • Cell proliferation is a very important physiological effect for low power laser irradiation (LPLI) used in clinical practice. (biomedcentral.com)
  • However, the mechanisms of cell proliferation induced by LPLI are poorly understood. (biomedcentral.com)
  • Recently, a large number of signaling proteins reported play an important key role in the process of LPLI-induced cell proliferation, probably due to the fact that the molecular events they are involved in are the basic response of the cells to extracellular stimuli. (biomedcentral.com)
  • The main advantage of hydrogels for microfluidic cell cultures, however, is their mimicry of extracellular matrix structures including adequate porosity for cellular organization, biocompatibility, and representative stiffness, all key parameters that promote native-like tissue function. (heraldopenaccess.us)
  • Despite recent achievements of microfluidic 2D cell culture systems [9], they still do not address the fact that in vivo cells coexist in 3D communities that are influenced by spatial orientation of cells and cell-to-cell contact within the extracellular matrix [10]. (heraldopenaccess.us)
  • Understanding that your Radiation coverage from your UMTS is also a couple of requests involving size less than from GSM, it is currently very easy to suggest that there's an general lowering of your publicity out of this interaction technique.Pulsed Electron-Electron Dual Resonance (PELDOR) upon double-stranded DNA A-1331852 cell line (ds-DNA) was adopted to look into your conformational flexibility involving helical DNA. (atpase-signal.com)
  • Use only Express Delivery (overnight) for cell line. (addexbio.com)
  • This cell line was derived from metastatic site: ascites. (addexbio.com)
  • The effect of phenethyl isothiocyanate (PEITC) on SNU449 hepatocellular carcinoma cell line cancer stem cells is not known. (fabad.org.tr)
  • Starting from single cell analysis using miniaturized flow cytometers [3] a variety of microfluidic devices have been developed for cell studies to investigate cell transport and cultivation in the absence and presence of concentration and temperature gradients or shear force conditions. (heraldopenaccess.us)
  • One of the major gene transfer vehicles, genetically modified viruses such as retrovirus, adenovirus, etc., have been utilized for approximately 80% of approved phase I clinical trials because of ease of transfer of their genome into the cells (19). (ualberta.ca)
  • These early steps consist of a long and perilous journey from the cell surface to the nucleus where the proviral DNA integrates into the host genome. (biomedcentral.com)
  • Retroviral particles must bind specifically to their target cells, cross the plasma membrane, reverse-transcribe their RNA genome, while uncoating the cores, find their way to the nuclear membrane and penetrate into the nucleus to finally dock and integrate into the cellular genome. (biomedcentral.com)
  • The serine/threonine kinase protein kinase D1 (PKD1) is a stress-responsive kinase and sensor for reactive oxygen species, which can initiate cell survival through NF-κB signaling. (biologists.com)
  • The present disclosure provides adeno-associated virus (AAV) virions with altered capsid protein, where the AAV virions exhibit greater infectivity of retinal cells compared to wild-type AAV. (justia.com)
  • Compared to control-treated cells, CD44 (3.2- fold) and p-STAT3 (2.44-fold) protein expressions were upregulated in PEITC-treated cells. (fabad.org.tr)
  • Finally, as proven by many examples in the past, progresses in retrovirology will undoubtedly also provide some priceless insights into cell biology. (biomedcentral.com)
  • Chitosan has a high potential for transferring DNA molecules into mammalian cells because of its cationic properties. (ualberta.ca)
  • Chitosan as a non-viral vehicle for transferring DNA molecules into the cells has recently attracted much attention because of its unique properties (7, 8). (ualberta.ca)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • however, recent developments have raised interest in nonpolypeptide regulators of immune cell trafficking belonging to a group of related molecules with a lysophospholipid-like structure. (ashpublications.org)
  • Gp120 binds to cells expressing CD4 cell-surface antigens, most notably T4-lymphocytes and monocytes/macrophages. (lookformedical.com)
  • The N-terminal part of gp41 is thought to be involved in CELL FUSION with the CD4 ANTIGENS of T4 LYMPHOCYTES, leading to syncytial formation. (lookformedical.com)
  • This viral capsid mediates the ability of AAV vectors to overcome many of the biological barriers of viral transduction-including cell surface receptor binding, endocytosis, intracellular trafficking, and unpackaging in the nucleus. (justia.com)
  • In the course of digestive tract inflammation, Th17 cells and immunosuppressive regulatory Capital t cells (Treg) are thought to try out Aurora Kinase inhibitor promotive and preventative roles, correspondingly. (atpase-signal.com)
  • 3-5 The immunological importance of these lysophospholipid receptors is illustrated by the profound immunodeficiency observed in mice lacking expression of the S1P 1 receptor in hematopoietic cells. (ashpublications.org)
  • Non-adherent and adherent cells have been grown in RPMI-1640 medium and DMEM or TC199 + 1. (estrogen-receptor.com)
  • The macrophageregulator GM-CSF, which can be made by some types of cancer cells [18,19], particularly induces HB-EGF in macrophages and neutrophils [20]. (estrogen-receptor.com)
  • Cell-free supernatants (SN) had been stored at -80 .Immunochemistry on tissues and cellsMethodsEthical requirementsThe blood and histological samples applied in our study have been in compliance with Institutional Review Board regulations.Cells and reagentsHistological samples were obtained by hepatic lobectomy to excise metastatic nodules derived from colon cancer. (estrogen-receptor.com)
  • We investigate the involvement of the kinin B1 and B2 receptors in metastatic breast tumor (4T1 breast cancer cells)-caused pain and in aromatase inhibitors (anastrozole or letrozole) therapy-associated pain. (bvsalud.org)
  • Curcumin causes cell death and primarily initiates an autophagic step (mitophagy). (hindawi.com)
  • These methods involve the use of genetically modified viruses to deliver the genetic material (11, 12), non-viral gene transfers including the direct physical introduction of the genetic material into cells (13-15) and formation of DNA complexes with inorganic salts, polycations, or lipids to transfer the DNA into the cells (16-18). (ualberta.ca)
  • The light must be absorbed by the endogenous chromophores of cells or tissues for actions. (biomedcentral.com)
  • The combination of 3-dimensional hydrogel cell cultures with microchip technology further allows (i) spatial and temporal control of cell growth, (ii) application of defined mechanical (e.g. shear, strain, stretch) and chemical (e.g. gradients) stimuli, as well as (iii) monitoring of dynamic cellular responses using integrated sensing strategies. (heraldopenaccess.us)
  • Compared to control confluence, gap fill, and migration rate were increased while half gap time was decreased in PEITC-treated cells. (fabad.org.tr)
  • Precise control of immune cell trafficking is complex and essential to the development and function of the immune system. (ashpublications.org)
  • Although several naturally occurring AAV variants have been isolated with a variety of tropisms, or cellular specificities, these vectors inefficiently infect Müller cells via intravitreal injection. (justia.com)
  • During the long journey from the cell surface to the nucleus, retroviruses will face multiple obstacles, since in addition to finding a path through the cytoplasm to the nucleus they have to cross two main barriers, the plasma and nuclear membranes, whilst at the same time avoiding or counteracting cellular defences that can interfere with many of these steps. (biomedcentral.com)
  • This review focuses primarily on recent advances in biologically inspired microfluidic systems that are based on 3-dimensional hydrogel cell cultures and discusses advantages and current challenges, various applications of microfluidic hydrogel cell cultures and future perspectives. (heraldopenaccess.us)
  • These microfluidic cell cultures are considered potential candidates to provide next generation cell analysis systems. (heraldopenaccess.us)
  • It has been repeatedly demonstrated that the presence of a 3D matrix promotes many biologically relevant functions otherwise not observed in 2D monolayer cell cultures [11]. (heraldopenaccess.us)
  • Start cultures at 2 X 10^5 cells/mL and maintain between 1 X 10^5 and 1 X 10^6 cells/mL. (addexbio.com)
  • The DNA binding activity of NFkB is activated and NFkB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. (sabbiotech.cn)
  • In addition, anti-cancerous agents, such as TNFα, ionizing radiation and chemotherapeutic drugs activate p50/p65 [ 15 , 16 ] leading to cell survival and consequently to drug resistance. (oncotarget.com)
  • 2 The mechanism of lysophospholipid-mediated cell migration recently has been shown to require the expression of specific GPCRs, such as the receptors for sphingosine 1-phosphate (S1P), lysophosphatidic acid (LPA), and LPC. (ashpublications.org)
  • Results of this study suggest that STAT3- mediated upregulation of CD44 leads to the gain of cancer stem cell phenotype of PEITC-treated SNU449 cells. (fabad.org.tr)
  • The main benefit of microfluidic systems for cell culture analysis is that they can perform a number of crucial liquid handling steps including cell loading, nutrient supply and waste removal under physiologically relevant shear force conditions, all while offering real time microscopy [4]. (heraldopenaccess.us)
  • Various methods have been developed to accomplish gene transfer into eukaryotic cells. (ualberta.ca)
  • The present disclosure further provides methods of delivering a gene product to a retinal cell in an individual, and methods of treating ocular disease. (justia.com)
  • Gene delivery of secretable neuroprotective factors to Müller cells, a type of retinal glia that contacts all classes of retinal neurons, represents an ideal approach to mediate protection of the entire retina. (justia.com)
  • Collectively, these types of benefits illustrate the very first time, to the understanding, in which (n-3) PUFA may regulate the particular colonic mucosal microenvironment in order to curb Th17 cell build up as well as inflamation related harm following a induction involving chronic colitis. (atpase-signal.com)
  • We show that in HeLa cells the 100 kDa 2-5 A synthetase can be specifically induced by short term treatments with TPA, or with the calcium ionophore A23187. (elsevierpure.com)
  • At higher doses, cells undergo mitochondrial destabilization due to calcium release from the endoplasmic reticulum, and die. (hindawi.com)
  • By contrast long term treatments by TPA resulting in the down regulation of PKC, or employing H7, a specific PKC inhibitor, reduced drastically the induction by IFNs of the 100 kDa enzyme in HeLa or fibroblast cells, without reducing the expression of the other forms. (elsevierpure.com)
  • FIGS. 10A-D depict expression of ShH10.Y445F.scCAG-GFP in Müller cells after intravitreal injection. (justia.com)
  • Men and women wild-type (WT) and also Fat-1 mice have been implemented dextran sea salt sulfate (DSS) within the mineral water (2.5%) in order to stimulate intense (Five n DSS) or long-term (3 series DSS) colitis and also the percentage of Treg along with Th17 cells existing locally [colonic lamina propria (cLP) as well as systemically (spleen) was resolute through movement cytometry. (atpase-signal.com)
  • These enzymes mediate part of the antiviral effects of IFNs and are thought to have an important role in cell growth or differentiation. (elsevierpure.com)
  • These SP constitute an important ingredient of cell walls and get harvested by suitable extraction or precipitation method, followed by purification, characterization and biological studies (Fig. 2 ). (springer.com)
  • Cancer stem cells play an essential role in resistance to therapy, invasion, metastasis, and recurrence. (fabad.org.tr)
  • Conclusion: Chitosan microparticles provide a sustained release of plasmid DNA for a long period and they have a potential for DNA transfer into the mammalian cells. (ualberta.ca)
  • Western blot: HeLa cells untreated or treated with Chloroquine. (arigobio.com)
  • Western blot: 30 µg of HeLa untreated or treated with Chloroquine at 50 µM, 100 µM (18 hr). (arigobio.com)
  • Nutritional (n-3) PUFA really modulate intestinal tract swelling partly through downregulating T-cell activation as well as features. (atpase-signal.com)