• The line is derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a 31-year-old African-American mother of five, who died of cancer on October 4, 1951, and after whom they are named. (wikipedia.org)
  • The family of Henrietta Lacks has reached a settlement with Thermo Fisher Scientific, a science and technology services company that profited by selling Lacks's cells. (acs.org)
  • They are called 'HeLa' cells from their initial host's name, Henrietta Lacks. (philosophynow.org)
  • Her name was Henrietta Lacks, but scientists know her as HeLa. (who.int)
  • Rhamnaceae) on cisplatin-induced damage to kidney epithelial LLC-PK1 cells via mitogen-activated protein kinase (MAPK) and apoptosis pathways. (mdpi.com)
  • In the current study, we exposed cultured human epithelial (HeLa) cells acutely and repeatedly to ozone and investigated the effects thereof on cell viability. (nwu.ac.za)
  • FOLR1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Mouse FOLR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6936) at 10 µg/mL for 3 hours at room temperature. (rndsystems.com)
  • Epithelial cells can be taken from the eye, urethra (remove purulent exudate), endocervix (remove purulent exudate), and rectum. (medscape.com)
  • Obtain specimen from epithelial cells in the infected site. (medscape.com)
  • The cells, known as HeLa cells, were taken from Lacks without her permission in 1951 while she was undergoing cervical cancer treatment at Johns Hopkins Hospital. (acs.org)
  • HeLa cells are an immortal line of human cervical cancer cells used in medical research. (philosophynow.org)
  • This is due to acute alterations in the tissues surrounding the tumor and in the environment as a result of treatment with anticancer therapeutics, which facilitate the drug resistance of tumor cells. (oncotarget.com)
  • There are many strains of HeLa cells as they continue to mutate in cell cultures, but all HeLa cells are descended from the same tumor cells removed from Lacks. (wikipedia.org)
  • They also indicate that the impact of VPA on cytotoxicity of etoposide in these tumor cells varies depending on the sequence of cultivation of the cells with the drugs. (nel.edu)
  • AuNPL cytotoxicity against HeLa, human malignant melanoma A375, and normal human keratinocytes HaCaT cells was tested by MTT cell survival assay, and their IC50 values were determined. (ac.rs)
  • The selectivity in the cytotoxic action of AuNPL on HeLa and A375 cancer cells compared with their cytotoxicity on normal keratinocytes HaCaT was observed. (ac.rs)
  • Hep G2 cell line human has been used to study the cytotoxicity of carbon monoxide-releasing molecules and organic solvents on HepG2 cells and to evaluate the antiproliferative activity of aqueous extracellular polysaccharides (AEPS) for HepG2 using the MTT colorimetric assay. (sigmaaldrich.com)
  • The cytotoxicity of etoposide and VPA to a UKF-NB-4 neuroblastoma cell line was evaluated with MTT assay. (nel.edu)
  • In contrast, the reversed combination (preincubation of the cells with VPA before their treating with etoposide) did not give any increase in etoposide cytotoxicity. (nel.edu)
  • Cytotoxicity of compounds was determined by MTT assay and viral load was assessed in cell substrates supernatants by plaque-forming and RT-qPCR assays. (bvsalud.org)
  • Access thousands of knockout cell lysates, generated from commonly used cancer cell lines. (abcam.com)
  • See here for more information on knockout cell lysates. (abcam.com)
  • Different strains of HeLa cells, all descended from that original sample, have been invaluable in a wide range of medical research ever since. (philosophynow.org)
  • The asexual cycle occurs in other mammals (including humans) and various strains of birds. (medscape.com)
  • Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1. (abcam.com)
  • To study the poliovirus-cell interaction, eight monoclonal antibodies that specifically block the cytopathic effects of poliovirus were generated by using HeLa cell preparations as immunogen and a newly developed colorimetric screening assay. (ncsu.edu)
  • For HeLa cell nucleus volume of 690 um^3 see BNID 104716 . (harvard.edu)
  • Although most of a eukaryotic cell's DNA is contained in the cell nucleus , the mitochondrion has its own genome ("mitogenome") that is substantially similar to bacterial genomes. (wikipedia.org)
  • It is also our view that there are no sound reasons for treating the early-stage human embryo or cloned human embryo as anything special, or as having moral status greater than human somatic cells in tissue culture. (wikiquote.org)
  • The KV in the zebrafish embryo contains cells with motile cilia that generate an anticlockwise fluid flow. (bmj.com)
  • Moreover, AAV9-NEM localized strongly in vivo to cells lining the calcified trabecular bone and transduced primary murine osteoblasts in culture, while WT AAV9 transduced undifferentiated bone marrow stromal cells as well as osteoblasts. (nature.com)
  • In terms of tissue specificity, AF3 detected the 100-kDa band in membrane preparations from human spinal cord but not in organ homogenates of human kidney or in murine tissue, including the central nervous system. (ncsu.edu)
  • The working concentration of blasticidin for mammalian cell lines varies from 1 to 10 μg/ml, in a few cases up to 30 μg/ml. (invivogen.com)
  • The cells were propagated by Gey shortly before Lacks died of her cancer in 1951. (wikipedia.org)
  • Novel luteolin capped gold nanoparticles (AuNPL) were synthesized and their anticancer effect towards human cervical adenocarcinoma HeLa cells was investigated in vitro. (ac.rs)
  • Impact of histone deacetylase inhibitor valproic acid on the anticancer effect of etoposide on neuroblastoma cells. (nel.edu)
  • Groh T, Hrabeta J, Poljakova J, Eckschlager T, Stiborova M. Impact of histone deacetylase inhibitor valproic acid on the anticancer effect of etoposide on neuroblastoma cells. (nel.edu)
  • Human ABC7 transporter: gene structure and mutation causing X-linked sideroblastic anemia with ataxia with disruption of cytosolic iron-sulfur protein maturation. (medlineplus.gov)
  • Other cell cultures were being invaded by suspected HeLa cells, so one research group contacted the Lacks family[when? (wikipedia.org)
  • To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. (sigmaaldrich.com)
  • At kinetochore-microtubule plus ends, the kinesin-8 family member Kif18A is essential to align mitotic chromosomes at the spindle equator during cell division, but how it accomplishes this function is unclear. (nih.gov)
  • The pink dots associated with each chromosome are the kinetochores which attach the chromosomes, at the centromere, to the spindle, allowing them to be pulled towards the opposite poles of the cell. (wellcomecollection.org)
  • Telomeres (in white) cap the ends of human chromosomes, protecting the genetic information from damage. (howstuffworks.com)
  • C(H)-C 6 H 4 OH- o , o -phen = 1,10-phenanthroline, and OAc = CH 3 COO − ) with human serum albumin (HSA) was studied using fluorescence quenching, synchronous and 3D fluorescence measurements and UV-vis spectroscopy. (rsc.org)
  • A deconvolved wide-field fluorescence microscope image of human HeLa cancer cells in metaphase of mitosis. (mitosispictures.com)
  • When cells were transfected with GFP-HIF-1α, treatment with TSA increased the number of green fluorescence protein (GFP)-positive cells. (oncotarget.com)
  • AuNPL exerted their cytotoxic activity against HeLa cells through accumulation of the cells in the subG1 phase of the cell cycle, inducing the apoptotic cell death mediated by the activation of caspase-3 − 8, and − 9. (ac.rs)
  • Etoposide and VPA were found as cycle phase specific drugs that are cytotoxic to human UKF-NB-4 neuroblastoma cells used either as single drugs or both together. (nel.edu)
  • However, whereas VPA might sensitize the cells to etoposide, inappropriate sequence of cultivation of the cells with VPA can decrease the etoposide cytotoxic efficacy. (nel.edu)
  • If you require a native form of the protein please use the live cell version - found here . (abcam.com)
  • We have purified and cloned a HeLa cell nuclear protein that strongly stimulates binding of retinoic acid and thyroid hormone receptors (RARs and TRs) to response elements. (nih.gov)
  • The purified protein is a human retinoid X receptor beta (hRXR beta). (nih.gov)
  • A major component of the regulation of histone protein synthesis during the cell cycle is the modulation of the half-life of histone mRNA. (umassmed.edu)
  • Wild-type (WT) AAV serotypes exhibit different tissue tropism due to different capsid protein sequences or variable regions interacting with disparate cell surface receptors, and thus can be utilized for different target tissues 3 . (nature.com)
  • Although snRNP profiles are not perturbed in SMN-depleted cells, we found that SMN depletion gives rise to cytoplasmic accumulation of a GFP-SmB reporter protein. (cnrs.fr)
  • Monoclonal antibody identification of a 100-kDa membrane protein in HeLa cells and human spinal cord involved in poliovirus attachment. (ncsu.edu)
  • The human Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species including plant Hsp60 (known as Rubisco binding protein), and bacterial GroEL, a major antigen of mycobacteria. (enzolifesciences.com)
  • SDS-PAGE analysis: Lane 1: MWM, Lane 2: 1.0 µg of purified Human Hsp60 protein. (enzolifesciences.com)
  • The ABCB7 protein is located in the inner membrane of cell structures called mitochondria. (medlineplus.gov)
  • In the mitochondria of developing red blood cells (erythroblasts), the ABCB7 protein plays a critical role in the production of heme. (medlineplus.gov)
  • Overall, researchers believe that the ABCB7 protein helps maintain an appropriate balance of iron (iron homeostasis) in developing red blood cells. (medlineplus.gov)
  • The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
  • Molecular docking studies indicated that all three complexes primarily bind to HSA in subdomain IIA with amino acid residues such as Arg218 and Lys199 which are located at the entrance of Sudlow's site I. The in vitro cytotoxicities of complexes 1-3 against HeLa cells showed promising anticancer activity (IC 50 = 3.5, 3.9 and 16.9 μM for 1 , 2 and 3 , respectively). (rsc.org)
  • This was the first human cell line to prove successful in vitro, which was a scientific achievement with profound future benefit to medical research. (wikipedia.org)
  • In an effort to further understanding of the effects of radiofrequency (RF) radiation on somatic cell mitotic activity and germ cell function, cell suspensions were exposed to 27 megahertz (MHz) or 2,450MHz radiation under isothermal conditions in-vitro. (cdc.gov)
  • Plaque-inhibition assays confirmed the viral specificity of the antibodies, and when one antibody, AF3, was used as a probe in immunoblots of cell membrane preparations, it detected a 100-kDa band in only those cell lines and tissues permissive for poliovirus infection. (ncsu.edu)
  • Furthermore, AF3 detected the band in a human-mouse hybrid cell line containing human chromosome 19, which confers permissivity for poliovirus infection, but the antibody did not detect the band in a human chromosome 19-deficient subclone. (ncsu.edu)
  • SIRT6 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SIRT6. (prosci-inc.com)
  • Antibody validated: Western Blot in human samples. (prosci-inc.com)
  • However, our understanding of the genetic causality for laterality defects in human beings remains relatively limited. (bmj.com)
  • Conclusions Our data implicate loss of MMP21 as a cause of heterotaxy in humans with concomitant defects in Notch signaling. (bmj.com)
  • Among immunodeficient individuals, toxoplasmosis most often occurs in those with defects of T-cell-mediated immunity, such as those with hematologic malignancies, bone marrow and solid organ transplants, or acquired immunodeficiency syndrome ( AIDS ).In most immunocompetent individuals, primary or chronic (latent) T gondii infection is asymptomatic. (medscape.com)
  • HeLa cell lines containing stably integrated copies of the fusion gene were synchronized by double thymidine block. (umassmed.edu)
  • Isolation and culture were crucial, as all emerging bacteria have been grown on artificial media or cell lines or at least propagated in animals. (cdc.gov)
  • seeking DNA samples to help identify contaminating cell lines. (wikipedia.org)
  • HeLa cells, like other cell lines, are termed "immortal" in that they can divide an unlimited number of times in a laboratory cell culture plate as long as fundamental cell survival conditions are met (i.e. being maintained and sustained in a suitable environment). (wikipedia.org)
  • HeLa, Detroit-6 and human amnion cell lines (all CL). (cdc.gov)
  • The suit lists several HeLa-related product lines that are currently for sale on Thermo Fisher's website, such as peptide and RNA standards . (acs.org)
  • Cell death induced by blasticidin occurs rapidly, allowing for selection of transfected cell lines carrying a blasticidin resistance gene within one week. (invivogen.com)
  • In addition, the method was optimized for viral quantification and tested in Vero, BHK-21, C6/36, LULO, and the Aedes cell lines. (bvsalud.org)
  • Insect cell lines represent a promising and expanding field as they have several research applications including biotechnology, virology, immunity, toxicology, cell signalling mechanisms and evolution. (bvsalud.org)
  • Although more than 1000 cell lines have currently been established from various insect species, Calliphora vicina-derived fly cell lines are lacking. (bvsalud.org)
  • Also, PIM cultured in some common cell lines to isolate other viruses of interest may unintentionally amplify poliovirus. (cdc.gov)
  • Respiratory or enteric viral isolates obtained from PIM specimens using poliovirus-permissive cell lines are also considered PIM. (cdc.gov)
  • It can be grown on any of these cell lines treated with cycloheximide: McCoy, HEp-2, HeLa, or buffalo green monkey cells. (medscape.com)
  • T cells stably expressing SARS-CoV Spike Glycoprotein. (topsan.org)
  • T6796CHO-K1 cells were stably infected with Human ACE2. (topsan.org)
  • T6029293T cells stably expressing Human ACE2Applications:For Research Use OnlyStorage Condition:Liquid nitrogen vapour phase. (topsan.org)
  • We have uncoupled transcriptional and posttranscriptional regulation by using a Drosophila hsp70-human H3 histone fusion gene that produces a marked human H3 histone mRNA upon heat induction. (umassmed.edu)
  • Distinct populations of H3 histone mRNA were produced by heat induction in early S-phase, late S-phase, or G2-phase cells, and the stability of the induced H3 histone mRNA was measured. (umassmed.edu)
  • Although Lacks, a Black woman, died 8 months later, her "immortal" cells live on and have contributed to scientific breakthroughs including the development of polio and COVID-19 vaccines. (acs.org)
  • The first "immortal" human cells grown in culture, they are still alive today, though she has been dead for more than sixty years. (who.int)
  • The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. (sigmaaldrich.com)
  • Dickkopf-3 alters the morphological response to retinoic acid during neuronal differentiation of human embryonal carcinoma cells. (nih.gov)
  • The Clinical Significance of Dickkopf Wnt Signaling Pathway Inhibitor Gene Family in Head and Neck Squamous Cell Carcinoma. (nih.gov)
  • A human breast cancer cell line that was derived in 1973 from the MALIGNANT PLEURAL EFFUSION of a patient with stage III BREAST CARCINOMA and was characterized as being of breast cancer origin. (bvsalud.org)
  • Functional characterization of an organic cation transporter (hOCT1) in a transiently transfected human cell line (HeLa). (drugbank.com)
  • The Ki values of several compounds in interacting with hOCT1 differed substantially from the corresponding values for the rat organic cation transporter, rOCT1, and the human kidney-specific organic cation transporter, hOCT2, determined in previous studies. (drugbank.com)
  • ABC transporter proteins carry many types of molecules across membranes in cells. (medlineplus.gov)
  • Cavadini P, Biasiotto G, Poli M, Levi S, Verardi R, Zanella I, Derosas M, Ingrassia R, Corrado M, Arosio P. RNA silencing of the mitochondrial ABCB7 transporter in HeLa cells causes an iron-deficient phenotype with mitochondrial iron overload. (medlineplus.gov)
  • Unique receptor sites for poliovirus are considered to be the primary determinant of the virus' cell and tissue-type specificity. (ncsu.edu)
  • Therefore, this study aims to design and evaluate a quantitative one-step RT-qPCR assay to detect CHIKV, ZIKV, and DENV with high specificity, reproducibility, and low cost in multiple cell substrates. (bvsalud.org)
  • Acute ozone exposure compromised cell membrane integrity severely, while adaptation to reverse an initial reduction in mitochondrial activity was observed. (nwu.ac.za)
  • These similarities are supported by studies where the single-ring human mitochondrial homolog, Hsp60 with its cochaperonin, Hsp10 were expressed in an E. coli strain, engineered so that the GroE operon is under strict regulatory control. (enzolifesciences.com)
  • All seeding densities should be based on cell counts gained by established methods. (abcam.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • It was observed that the cells grew robustly, doubling every 20-24 hours unlike previous specimens that died out. (wikipedia.org)
  • But the agreement is likely not the last and is already prompting debate over the use of human specimens in scientific research. (acs.org)
  • In direct ELISAs, approximately 30% cross-reactivity with recombinant human FOLR1 is observed. (rndsystems.com)
  • Thermo Fisher, for example, "has made staggering profits by using the HeLa cell line-all while Ms. Lacks' Estate and family haven't seen a dime," the lawsuit , filed in 2021, says. (acs.org)
  • Typically, mammalian cells are sensitive to blasticidin concentrations of 1-10 µg/ml, and bacteria to 25-100 µg/ml. (invivogen.com)
  • Using this method, we examined the genomic positions of 6mA in bacteria ( E. coli ), green algae ( C. reinhardtii ), and mammalian cells (HEK239T, Huh7, and HeLa cells). (nature.com)
  • In contrast to bacteria and green algae, human cells possess a very limited number of 6mA sites which are sporadically distributed across the genome of different cell types. (nature.com)
  • The genetic etiology of human NTDs remains poorly understood despite intensive investigation. (cdc.gov)
  • Dr Heidi Mattock and use genetic data to identify the etiology of human canceRs. (who.int)
  • Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose. (abcam.com)
  • Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. (sigmaaldrich.com)
  • This study was aimed at establishing a new C. vicina embryonic tissue-derived cell line. (bvsalud.org)
  • A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)
  • Characterization of a human gene with sequence homology to Saccharomyces cerevisiae Sir 2. (prosci-inc.com)
  • Additionally, as HeLa cells were popularized and used more frequently throughout the scientific community, Lacks's relatives received no financial benefit and continued to live with limited access to healthcare. (wikipedia.org)
  • Additionally, AuNPL were shown as more cytoprotective towards HaCaT cells then luteolin due to the more successful elimination of H2O2 induced intracellular ROS. (ac.rs)
  • Total apoptosis (about 12%) and no significant alteration in cell cycle were confirmed for SGE of A. variegatum and R. appendiculatus, and additionally SGE of 5-day fed I. ricinus females (IC(50) 0.64 mg/ml). (nel.edu)
  • Furthermore, AF3 reacted with human peripheral mononuclear cells, consistent with the known replication of poliovirus in Peyer's patches and tonsils. (ncsu.edu)
  • Cell types included resting human peripheral lymphocytes, LN71 or RT2 glioma cells, Chinese-hamster-ovary cells, HeLa cells, and mouse spermatozoa. (cdc.gov)
  • Peripheral blood biomarkers of cell-specific autoimmunity. (lu.se)
  • Given that we have an efficiency of 1% cloning for livestock species and if only one in a thousand cells are viable then around 100,000 cells would need to be transferred. (wikiquote.org)
  • More than one hundred clinical trials using AAV drugs have been conducted to correct human defective genes, making AAV the most popular gene delivery system, mostly due to their relatively low immunogenicity, customizable design, and safety profile. (nature.com)
  • Modified versions of AAVs range from genetic alterations of the viral cap gene to conjugation of proteins such as streptavidin or antibodies to promote interaction with specific cells. (nature.com)
  • 1991. Blasticidin S-resistance gene (bsr): A novel selectable marker for mammalian cells. (invivogen.com)
  • 1994. Blasticidin S deaminase gene from Aspergillus terreus (BSD): a new drug resistance gene for transfection of mammalian cells. (invivogen.com)
  • The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
  • For example, microarray technology has been used to compare gene expression profiles between normal and diseased cells and this has led to dramatic advances in the understanding of cellular processes at the molecular level [ 1 ]. (biomedcentral.com)
  • mutations high frequency of IDH1/2 mutations in oligodendrogliomas, astrocytomas and in alteRations in the RB1 pathway in The TET2 gene encodes the -KG- secondary glioblastomas derived thereof low-gRade diffuse gliomas lacking dependent enzyme that catalyses suggests that these tumours share a common genetic alteRations the conversion of 5-methylcytosine to common progenitor cell population. (who.int)
  • Maul GG, Deaven L. Quantitative determination of nuclear pore complexes in cycling cells with differing DNA content. (harvard.edu)
  • These cells are treated as cancer cells, as they are descended from a biopsy taken from a visible lesion on the cervix as part of Lacks's diagnosis of cancer. (wikipedia.org)
  • By inhibiting the topoisomerase II enzyme activity in cancer cells, this drug leads to DNA damage and subsequently to cell death. (nel.edu)
  • Differential regulation by melatonin of cell growth and androgen receptor binding to the androgen response element in prostate cancer cells. (nel.edu)
  • The doctors who treated her took a sample of her cancer cells. (philosophynow.org)
  • Henrietta's cancer cells unexpectedly did not die after a few hours - more the opposite: it was found that if nourished sufficiently they would survive and could be reproduced indefinitely. (philosophynow.org)
  • Our findings suggest that kinesin-8 family members have developed biochemically distinct activities throughout evolution and have implications for how Kif18A affects kinetochore-microtubule plus-end dynamics during mitosis in animal cells. (nih.gov)
  • Communication between tissue donors and doctors was virtually nonexistent-cells were taken without patient consent, and patients were not told what the cells would be used for. (wikipedia.org)
  • caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death. (enzolifesciences.com)
  • Anti-proliferative activity and apoptotic effect of tick salivary gland extracts on human HeLa cells. (nel.edu)
  • The anti-proliferative and apoptotic potential of salivary gland extracts (SGE) of unfed and partially fed adult ixodid ticks were screened using human HeLa cells. (nel.edu)
  • Annexin V-FITC was used to detect apoptotic cells. (nel.edu)
  • The study shows that tumour cell growth suppressant(s) and/or apoptotic triggers are present in tick salivary glands, however, further research into the characteristics and mode of action of such natural substances is necessary. (nel.edu)
  • Kazimírová M, Dovinová I, Rolníková T, Tóthová L, Hunáková L. Anti-proliferative activity and apoptotic effect of tick salivary gland extracts on human HeLa cells. (nel.edu)
  • In an immunohistochemical study of the human brainstem, AF3 stained neurons in the reticular formation and clusters of brainstem neurons, consistent with the known pattern of damage caused by poliovirus infection in the brainstem. (ncsu.edu)
  • The infection produces a wide range of clinical syndromes in humans, land and sea mammals, and various bird species. (medscape.com)
  • T gondii oocysts, tachyzoites, and bradyzoites can cause infection in humans. (medscape.com)
  • Effects of 27 MHz radiation on somatic and germ cells. (cdc.gov)
  • HeLa, mouse L, and CHO cells were grown in suspension culture. (harvard.edu)
  • After knocking out the RNA m 6 A methyltransferase METTL3 in mouse ES cells, 6mA becomes mostly diminished. (nature.com)
  • Mitochondria are involved in a wide variety of cellular activities, including energy production, chemical signaling, and regulation of cell growth and division. (medlineplus.gov)
  • We tested the interaction of an array of organic cations and other compounds with hOCT1 by determining Ki values in inhibiting 14C-tetraethylammonium (TEA) transport in the transfected cells. (drugbank.com)
  • 1 Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, 465 21st Avenue South, Nashville, TN 37232-8240, USA. (nih.gov)
  • We evaluated recent journal articles in the fields of Neuroscience, Developmental Biology, Immunology, Cell and Molecular Biology and General Biology, selected randomly based on a diversity of impact factors for the journals, publishers, and experimental method reporting guidelines. (researchgate.net)
  • 2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. (abcam.com)
  • Add thawed cells to a conical based centrifuge tube, e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. (sigmaaldrich.com)
  • Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. (sigmaaldrich.com)