• They did not interfere with the expression of human papillomavirus oncogenes integrated in the HeLa cell genome, but they did induce strong G1 arrest and result in the activation of caspase-3 and apoptosis. (springer.com)
  • Last March, German scientists published the HeLa genome - again without consent from Henrietta's family - and controversy erupted . (npr.org)
  • The NIH then set up a committee that included the Lacks family to figure out a way to publish the HeLa genome, while still respecting the family's privacy. (npr.org)
  • A portion of the HeLa genome has also been published without familial consent. (labroots.com)
  • This MOA was also confirmed experimentally: the cyano-substituted heteroaryles disrupted the actin and the tubulin networks in HeLa cells and inhibited cellular migration. (springer.com)
  • Bottom row: HeLa cells were treated with low (left) or high (right) concentrations of staurosporine and assayed for cytotoxicity with the EarlyTox Cell Integrity Kit. (moleculardevices.com)
  • Our model was also in agreement with experiments showing that the number of HSF molecules in a HeLa cell is roughly 100 times greater than the number of stress-activated heat-shock element sites, further confirming the model's ability to reproduce experimental results not used in model calibration. (nih.gov)
  • The National Institutes of Health and the Lacks family have agreed to give scientists access to the genetic sequence of the cells, with some restrictions to safeguard her relatives' privacy. (npr.org)
  • The scientists set of a revolution in biomedical research with Lacks' cells, which took their name, HeLa, from hers. (labroots.com)
  • HeLa cells were seeded at 4 initial densities in a 96-well plate: 250 (purple), 500 (blue), 1000 (green), and 2000 (red) cells per well. (moleculardevices.com)
  • HeLa cells were seeded at 1000 cells per well and allowed to grow overnight. (moleculardevices.com)
  • To determine a suitable concentration for selection:Plate non-transfected control cells at a low density (1E4/ml for example)The next day, add G418 at 0, 100, 200, 400, 600, 800, 1000, 1200 ug/mlIncubate the cells for 6 days (may need to refeed)Check cells under microscope. (protocol-online.org)
  • Furthermore, a second in silico investigation revealed that the tumor cells that are sensitive to the cyano-substituted compounds show differences in their expression of locomotion genes compared with that of insensitive cell lines, thus corroborating the involvement of the cytoskeleton. (springer.com)
  • A computational analysis was performed that compared the antiproliferative activities of our compounds in 13 different tumor cell lines with those of compounds listed in the National Cancer Institute database. (springer.com)
  • Working with members of the Lacks family, the University of Washington is the first to publish information on the HeLa cells under the new guidelines. (thefreedictionary.com)
  • HeLa cells have been used extensively around the world in many different fields of research including cancer research, immunology and vaccine development. (wellcomecollection.org)
  • The cell cycle has been extensively studied for years which led to greater understanding of life of a cell and organism. (nature.com)
  • EGFR is a cell surface protein that binds to epidermal growth factor. (novusbio.com)
  • After more than 50 years, there are now billions and billions of HeLa cells in laboratories all over the world. (howstuffworks.com)
  • These HeLa cells are so important that several laboratories have spent years trying to sequence their DNA. (npr.org)
  • Recent advances in single-cell omics and other techniques are revealing variation at genomic, epigenomic, transcriptomic, and posttranscriptomic levels. (the-scientist.com)
  • And while the cells became commercialized (researchers can buy a vial of them for $250) Lacks' family has lived without healthcare and in poverty. (howstuffworks.com)
  • For a long time, researchers who were curious enough to ask were told that HeLa cells were named after 'Helen Lane' or 'Helen Larson. (howstuffworks.com)
  • None of the researchers working on HeLa cells ever took the time to educate the family about what was really going (RadioLab, 2010). (thefreedictionary.com)
  • A biopsy of her cells that was taken during her clinical consultations was later used, without her knowledge, by researchers. (labroots.com)
  • Therefore, we used H2 ER [alpha]-GFP transfected into HeLa cells to test D3 binding by visualizing that D3 increases the translocation of ER [alpha] to the nucleus. (thefreedictionary.com)
  • Decreasing impedance magnitude of the single HeLa cell increases measurement sensitivity, but higher operational voltage will damage single HeLa cell. (hindawi.com)
  • The latter interaction is essential for kinetochore localization of Bub1 and its function in cell cycle arrest induced by spindle assembly checkpoint (SAC) activation. (wikipedia.org)
  • C )MS/MS/MS spectrum of the neutral loss precursor ion from B . Abundant peptide bond fragmentation permitted the unambiguous identification of this peptide from the protein cell division cycle 2-related protein kinase 7, with a phosphorylated Ser residue marked by an asterisk. (pnas.org)
  • The present study demonstrates the practicability of CdSe QDs as an attractive type of fluorescent labels for biological applications such as protein probes and cell imaging. (biomedsearch.com)
  • The protein kinase Bub1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase. (wikipedia.org)
  • The concentration of G418 which just kills all the cells is suitable for use in subsequent selection procedures. (protocol-online.org)
  • Once the killer cell has killed some of the HeLa cells, the killer cell is gently detached from the HeLa layer with the CellEctor microcapillary for subsequent molecular analysis. (selectscience.net)
  • Nanoviscosity of the cytoplasm is a key factor affecting diffusion of biomolecules and - as a consequence - rates of biochemical reactions in a cell. (nature.com)
  • In this paper we address this topic by constant observation of nanoviscosity of HeLa cells cytoplasm during S, G2 and G1 phases after Aphidicolin synchronization. (nature.com)
  • Our results hint at possible existence of robust mechanism maintaining stable physiological viscosity of the cytoplasm, despite huge structural changes during cell cycle. (nature.com)
  • Cell lines are used in all kinds of ways, such as studying the effects of diseases or developing medications and vaccines, and play an invaluable role in medicine today. (howstuffworks.com)
  • 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines. (novusbio.com)
  • The cell cycle of somatic cells is commonly divided into two periods: interphase and mitosis. (nature.com)
  • It was expected, that significant structural changes in cells during biosynthesis or mitosis would have an impact on nanoviscosity, and further - diffusive motion of biomolecules. (nature.com)
  • In humans Bub1 accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. (wikipedia.org)
  • In the cell in the centre of the image, condensed chromosomes (purple) have aligned and attached to the spindle (green). (wellcomecollection.org)
  • HeLa cells are incredibly hardy, which makes them very useful for medical research. (howstuffworks.com)
  • The fact that HeLa cells have been used in some very important, groundbreaking medical research is interesting enough, but there's another part of the story -- and that part is why Oprah might be making a movie about HeLa. (howstuffworks.com)
  • With excellent optical properties, quantum dots (QDs) have been made as attractive molecular probes for labelling cells in biological research. (biomedsearch.com)
  • The cells are among the most widely used in research. (npr.org)
  • These cells are among the most widely used in biomedical research worldwide. (npr.org)
  • Those unique and important cells were the first to be cultured and reproduced in a laboratory. (labroots.com)
  • For example I found out that the test my daughter had to confirm a diagnosis of Williams Syndrome was made possible by HeLa cells ," she says. (thefreedictionary.com)