• Apoptosis profile shows (shown that) many cells undergoing apoptosis after treatment. (chemoprev.org)
  • Thus, EBMD potentially inhibits activation of NF-κB pathway and triggers apoptosis on HeLa cells. (chemoprev.org)
  • In the present study we investigated the efficacy of Organic extracts of Nigella sativa seed powder for its clonogenic inhibition and induction of apoptosis in HeLa cancer cell. (biomedcentral.com)
  • All three extracts induced apoptosis in HeLa cells. (biomedcentral.com)
  • Apoptosis an active physiological process resulting in cellular self-destruction of unwanted cells is absent in cancer cells. (biomedcentral.com)
  • Rhamnaceae) on cisplatin-induced damage to kidney epithelial LLC-PK1 cells via mitogen-activated protein kinase (MAPK) and apoptosis pathways. (mdpi.com)
  • Cell cycle analysis suggested activation of apoptosis, without arresting cell cycle progression. (nature.com)
  • Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
  • The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. (abcam.com)
  • This gene encodes a nuclear matrix protein, which is proposed to stabilize certain messenger RNA species. (nih.gov)
  • Purification and partial sequencing of the nuclear autoantigen RA33 shows that it is indistinguishable from the A2 protein of the heterogeneous nuclear ribonucleoprotein complex. (jci.org)
  • Sequence analysis of five tryptic peptides revealed that their sequences matched corresponding sequences of the A2 protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. (jci.org)
  • The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
  • In addition, by South-western blotting analysis of nuclear extracts using the 95-bp probe or MRE oligonucleotide probe, we detected a Zn-dependent DNA-binding protein with a molecular mass of 116 kDa, which is likely to be ZRF. (go.jp)
  • Analysis of HeLa cell nuclear proteins fractionated by glycerol gradient centrifugation showed that ZRF is distinct from another MRE-binding protein, MREBP. (go.jp)
  • We therefore asked what role JMJD1B plays in newly synthesized histone maturation by exploiting the knockdown of JMJD1B by treating HeLa cells with siRNA against JMJD1B (siJMJD1B) and observed the reduction of both JMJD1B protein on cytosolic extracts (Fig. 1 b) and mRNA levels (Fig. 1 c) 72 h after the treatment. (biomedcentral.com)
  • Polypyrimidine tract binding protein and associated splicing factor (PSF) has diverse functions inside the cell such as transcriptional regulation, DNA repair, acts as nucleic acids binding protein and regulate replication and infectivity of different viruses. (biomedcentral.com)
  • In the present study, we have identified a new HIV-1 integrase interacting protein, polypyrimidine tract binding protein and associated splicing factor (PSF) through pull down assay and co immunoprecipitation of IN with mammalian cell protein. (biomedcentral.com)
  • Removal of the U-type snRNPs from the nuclear extracts of HeLa cells with protein A-Sepharose-coupled human autoimmune antibodies leads to complete loss of splicing activity. (unibas.ch)
  • Thus, the control of ribosome assembly and protein synthesis is essential for the survival of cancer cells. (biomedcentral.com)
  • In this context, during any sort of stress condition the alternative reading frame protein p14ARF subtracts MDM2, an E3 ubiquitin ligase, from the interaction with p53 in manner to free and stabilise p53 level in the nucleolus of the cell where it inhibits RNA polymerase I activity [ 3 ]. (biomedcentral.com)
  • When cells were transfected with GFP-HIF-1α, treatment with TSA increased the number of green fluorescence protein (GFP)-positive cells. (oncotarget.com)
  • TSA also enhanced the nuclear translocation of HIF-1α protein, as assessed by immunoblotting and as evidenced by increased nuclear localization of GFP-HIF-1α. (oncotarget.com)
  • We show that hSSB1 depleted cells contain less BLM protein and that this deficiency is due to proteasome mediated degradation of BLM. (biomedcentral.com)
  • Our data highlights that BLM helicase and hSSB1 function in a dynamic complex in cells and that this complex is likely required for BLM protein stability and function. (biomedcentral.com)
  • The spike protein of coronaviruses mediates virus binding and cell entry. (cdc.gov)
  • Treatment of human Hep G2 cells with 1 ng/ml TNF-alpha increased [14C]putrescine cross-linking to cellular proteins. (nih.gov)
  • In vivo studies further confirmed the inhibitory effect of celastrol on the expression of HIF-1α proteins, leading to a decreased growth of Hep3B cells in a xenograft tumor model. (spandidos-publications.com)
  • These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)
  • To study the interactions between these sequences and regulatory factors, HeLa cell nuclear proteins were analyzed by band-shift assay using a 95-base pair (bp) DNA probe containing a part of the human MT-IIA gene upstream sequences. (go.jp)
  • For isolation of wt-p68 and its mutants, COS cells (1 108), transfected with the respective manifestation plasmids for 4 days, were extracted as explained by (5) except the nuclear extraction buffer contained 4?mM EDTA, which was subsequently removed by dialysis before the recombinant proteins were purified by affinity chromatography on Ni2+ NTACcellulose and ssDNACcellulose as described (5). (mergullo.net)
  • Generally, these receptors explain their function on the cell membrane where, after the binding with growth factors or neuregulin undergo to homo or hetero oligomerization with the activation of the intrinsic tyrosine kinase activity and the subsequent recruitment of proteins involved in the cytoplasmic signalling pathways. (biomedcentral.com)
  • Cell 75:495, 1993), and a series of unique E6-AP homologous proteins have been identified (Huibregtse et al. (justia.com)
  • Cell-based and in vitro assays show that Retro-2 blocks delivery of newly-synthesized TA-proteins to the ER-targeting factor ASNA1 (TRC40). (elifesciences.org)
  • We found an activity in lipidic extract from Iris germanica that was able to increase HeLa cell area and adhesion and augment the formation of actin stress fibers. (iucc.ac.il)
  • Total RNA was extracted from untreated (WT), cdk9 MO-injected and cdk9m MO-injected embryos. (echinobase.org)
  • Total RNA was extracted from untreated (WT: lanes 1-3), cdk9 MO-injected ( cdk9 MO: lanes 4-6) and cdk9m MO-injected embryos (cdk9m MO: lanes 7-9). (echinobase.org)
  • The total RNA was extracted, and a PAT assay was performed with PAT primers for cdk9 and tbp . (echinobase.org)
  • From all aliquots, total RNA was extracted as explained above and the integrated radioactivity measured by liquid scintillation counting. (mergullo.net)
  • HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro. (abcam.com)
  • the CNS of B7-H1-deficient mice confirmed inhibition of CD8 T cell function in vivo. (jak-inhibitors.com)
  • The inhibition of splicing can be prevented by saturating the coupled antibodies with purified nucleoplasmic U snRNPs prior to incubation with nuclear extract. (unibas.ch)
  • Cannabis sativa in particular cannabidiol, we propose it plays important role in helping the body fight cancer through inhibition of pain and cell growth. (biomedcentral.com)
  • Molecular Cell. (wikipedia.org)
  • RA33 is a nuclear autoantigen with an apparent molecular mass of 33 kD. (jci.org)
  • In astrocytes the OGD enhanced the nuclear translocation of hypoxia-inducible factor 1 (HIF-1), the major transcription factor regulating EPO expression. (jneurosci.org)
  • Here, we present a quantitative and comprehensive study of the abundance levels of histone PTMs during the differentiation of mouse embryonic stem cells (ESCs) using mass spectrometry (MS). We observed dynamic changes of histone PTMs including increased H3K9 methylation levels in agreement with previously reported results. (biomedcentral.com)
  • Methanolic, n-Hexane and chloroform extracts of Nigella sativa seedz effectively killed HeLa cells. (biomedcentral.com)
  • The IC 50 values of methanolic, n-hexane, and chloroform extracts of Nigella sativa were 2.28 μg/ml, 2.20 μg/ml and 0.41 ng/ml, respectively. (biomedcentral.com)
  • Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
  • We did not observe any significant difference between the amount of early reverse transcription products as well as infectivity of virus in the PSF KD and control TZM-bl cells. (biomedcentral.com)
  • Splicing factor such as Serine Arginine rich splicing factor (SRSF1) [ 16 ] regulate HIV-1 transcription whereas, Small nuclear RNAs [ 17 ] inhibit HIV-1 replication through excessive RNA Splicing. (biomedcentral.com)
  • Suggesting the presence of a bypass activity, the block to elongation is alleviated when transcription is carried out in HeLa cell nuclear extracts. (elsevierpure.com)
  • Tumor suppressors, oncogenes and alternatively deregulated upstream signalling pathways can directly influence the RNA polymerase I activity inducing hyper activation of rRNA transcription in cancer cells. (biomedcentral.com)
  • NF-κB is a transcription factor that plays a pivotal role in the highly specific pattern of gene expression observed for immune, inflammatory and acute phase response genes, including interleukin 1, interleukin 8, tumor necrosis factor and certain cell adhesion molecules. (justia.com)
  • HeLa cells are human epithelial cells from a fatal cervical carcinoma. (abcam.com)
  • Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. (abcam.com)
  • Hep G2 cells, transiently transfected with a luciferase reporter containing 1.67 kb of the human tTG promoter, showed an increase in reporter activity after addition of TNF-alpha. (nih.gov)
  • Comparison of nuclear splicing extracts from human HeLa and Drosophila Kc cells has revealed species-specificity, consistent with the observed size differences. (umd.edu)
  • As observed with the 74 nucleotide second intron of the Drosophila white gene, the wild-type myosin intron is accurately spliced in a homologous extract, and increasing the size by 16 nucleotides both eliminates splicing in the Drosophila extract and allows accurate splicing in the human extract. (umd.edu)
  • In contrast to previous results, however, an upstream cryptic 5' splice site is activated when the wild-type myosin intron is tested in a human HeLa cell nuclear extract, resulting in the removal of a 98 nucleotide intron. (umd.edu)
  • Celastrol showed a potent inhibitory activity against HIF-1 activation induced by hypoxia in various human cancer cell lines. (spandidos-publications.com)
  • It is the first continuous human cell line. (apicells.com)
  • We have identified an abundant transcript initiated upstream from the canonical cap site of human α1 globin gene in bone marrow cells and in COS-7 cells transfected with an α1 globin gene-containing plasmid. (tmu.edu.tw)
  • Maintenance of genome stability is critical in human cells. (biomedcentral.com)
  • Ab blockade of B7-H1 on oligodendroglia in vitro enhanced IFN-gamma secretion by virus-specific CD8 T cells. (jak-inhibitors.com)
  • OBJECTIVE: It is necessary to improve biotech platforms based on in vitro cell tissue culture to support sacha inchi (Plukenetia volubilis L.) research programs and draw on the nutritional value of the high polyunsaturated fatty acid content of its oilseed. (bvsalud.org)
  • It is also synthesized efficiently in vitro by RNA polymerase II in the nuclear extracts prepared from a Hela cell line and an erythroleukemia cell line, K562. (tmu.edu.tw)
  • We have investigated the role of small nuclear ribonucleoprotein particles (snRNPs) in the in vitro splicing of messenger RNA precursors by a variety of procedures. (unibas.ch)
  • Pluripotent cells can be differentiated into many different cell types in vitro. (biomedcentral.com)
  • Histone acetylation has been implicated to be important for the pluripotent cell state, as some HDAC inhibitors have been shown to improve reprogramming efficiency of induced pluripotent stem cells (iPSCs) or maintain a pluripotent stem cell state. (biomedcentral.com)
  • Activation and nuclear translocation of NF-κB occurs following signal-induced phosphorylation of IκB, which leads to proteolysis via the ubiquitin pathway. (justia.com)
  • Gel shift experiments using nuclear extracts from TNF-alpha-treated cells and oligonucleotides containing the tTG nuclear factor (NF)-kappa B motif revealed increased binding, concordant with mRNA data. (nih.gov)
  • For fluorescein-transport experiments, the apparent permeability (P-app) of fluorescein in Caco-2 cells pretreated with 20 mu M TFs were significantly decreased compared with that in untreated cells. (jak-inhibitors.com)
  • As your recommendaion, the Jurkat cells treated with anti Fas antibody to induce cleavage is positive control. (abcam.com)
  • Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.cn)
  • Therefore we recommend western blots using nuclear extracts and running Hypoxia treated samples as positive control ( ab180880 ). (abcam.cn)
  • RNAs isolated from these cell lines, however, do not contain this upstream transcript. (tmu.edu.tw)
  • The development of teeth is initiated by epithelial-mesenchymal interactions, which form dental papilla cells and dental sac cells, subsequently forming pulp, dentin, cementum, periodontal ligament and proper alveolar bone, except enamel 1 - 4 . (researchsquare.com)
  • In the eukaryotic cells the production of ribosomes takes place in the nucleolus, a specialized subnuclear compartment where the RNA Polymerase I (RNA Pol I) catalyses the ribosomal RNA synthesis (rRNA). (biomedcentral.com)
  • We investigated its cellular localization and found it in both cytosolic and nuclear extracts derived from HeLa cells (Fig. 1 a). (biomedcentral.com)
  • The siRNA knockdown (KD) of PSF resulted in increased viral replication in TZM-bl cells, suggesting PSF has negative influence on viral replication. (biomedcentral.com)
  • The quantitative PCR of virus infected PSF knockdown TZM-bl cells showed more integrated DNA and viral cDNA as compared to control cells. (biomedcentral.com)
  • An aggressive HeLa, a metastatic ME-180 and a primary SiHa cell lines were purchased from ATCC (USA, MD). Camptothecin was supplied by Calbiochem® and cannabidiol was purchased from Sigma-Aldrich and used as a standard reference. (biomedcentral.com)
  • In conclusion, these data suggest that cannabidiol rather than Cannabis sativa crude extracts prevent cell growth and induce cell death in cervical cancer cell lines. (biomedcentral.com)
  • In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
  • In our previous studies, EMSCs were obtained from rat embryonic facial process tissue by fluorescence p75 neurotrophin receptor (p75NTR) activated cell sorting, providing a good stem cell model for studies of dental mineralization. (researchsquare.com)
  • Maintaining a proper supply of soluble histones throughout the cell cycle is important to ensure chromatin and genome stability. (biomedcentral.com)
  • Moreover, despite control of infectious virus, CD8 T cells within the CNS of persistently infected mice maintained Selleck PLX3397 high PD-1 expression. (jak-inhibitors.com)
  • Several major species of E2 enzymes have been characterized in mammalian cells, plants, and yeast. (justia.com)
  • mRNA quality is controlled by multiple RNA surveillance machineries to reduce errors during gene expression processes in eukaryotic cells. (molcells.org)
  • Eukaryotic cells destroy many endocytosed pathogens by targeting them to the lysosome. (elifesciences.org)
  • Nucleolar activity is also influenced by the interaction between pathways activated from extracellular signals in order to coordinate ribosome synthesis and cell proliferation [ 6 ]. (biomedcentral.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Opposing function of MYBBP1A in proliferation and migration of head and neck squamous cell carcinoma cells. (nih.gov)
  • Instead ErbB3 silencing or nucleolar stress reduce cell proliferation and affect cell cycle progression. (biomedcentral.com)
  • These data point to the existence of an ErbB3-mediated non canonical pathway that glioblastoma cells use to control ribosomes synthesis and cell proliferation. (biomedcentral.com)
  • Results obtained indicate that both cannabidiol and Cannabis sativa extracts were able to halt cell proliferation in all cell lines at varying concentrations. (biomedcentral.com)
  • These compounds are almost structurally similar or possess identical pharmacological activities and offer various potential applications including the ability to inhibit cell growth, proliferation and inflammation [ 22 ]. (biomedcentral.com)
  • pointed out that Mage-D1 may participate in the proliferation of bone marrow mesenchymal stem cells (BMSCs) and the differentiation of odontoblasts through the NF-κB signalling pathway 21 . (researchsquare.com)
  • This is due to acute alterations in the tissues surrounding the tumor and in the environment as a result of treatment with anticancer therapeutics, which facilitate the drug resistance of tumor cells. (oncotarget.com)
  • Both cell-derived microstructures and extracellular matrix components play critical roles in the preorganization and oriented deposition of calcium phosphate and serve as passive supports in dentine and enamel. (researchsquare.com)
  • we noted that a naturally larger (150 nucleotide) intron from the ftz gene is efficiently spliced in Kc cell extracts that do not splice enlarged introns (of 84, 90, 150 or 350 nucleotides) derived from the 74 nucleotide white intron. (umd.edu)
  • p53, a tumor suppressor gene, helps regulate the cell cycle. (biomedcentral.com)
  • Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. (thermofisher.com)
  • Timely differentiation of progenitor cells is critical for development. (rupress.org)
  • In this study we asked whether global epigenetic mechanisms regulate timing of progenitor cell differentiation into myelin-forming oligodendrocytes in vivo. (rupress.org)
  • This hypothesis implied that OL differentiation proceeded by default once the cells exited from the cell cycle. (rupress.org)
  • CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. (thermofisher.com)
  • Together, our data demonstrate the fundamental role of Brd4 in monitoring cell differentiation through its interaction with acetylated histone marks and disruption of Brd4 may cause aberrant differentiation. (biomedcentral.com)
  • This research is to investigate anticancer mechanism of Phaleria macrocarpa fruits extracts (EBMD) in NF-κB pathway. (chemoprev.org)
  • Homologous recombination (HR) is a major DNA repair pathway that may be employed for the repair of double-strand DNA breaks (DSBs) during S and G2 of the cell cycle [ 2 ]. (biomedcentral.com)
  • DNA information was measured by fluorescence activated cell sorting using a movement cytometer FACScalibur. (wee1signaling.com)
  • We investigated the effect of theaflavins (TFs) on membrane barrier of Caco-2 cells. (jak-inhibitors.com)
  • The nucleolus is a subnuclear, non-membrane bound domain that is the hub of ribosome biogenesis and a critical regulator of cell homeostasis. (biomedcentral.com)
  • Intensities of c Met immunoreactivity had been defined as: 0, total absence of membrane staining or membrane staining in under 30 of cancer cells, 1t, faint and partial membrane staining in not less than 30 of cancer cells, 2t, robust and finish staining in at least 30 of cancer cells. (wee1signaling.com)
  • It is largely undetectable in cells or tissues grown under normoxic conditions. (abcam.cn)
  • Western blotting was performed with extracts from untreated (lanes 1, 4, 7 and 10), cdk9 MO-injected (lanes 2, 5, 8 and 11) and cdk9m MO-injected embryos (lanes 3, 6, 9 and 12) by using the antibodies indicated. (echinobase.org)
  • The extracts were subjected to western blotting by using antibodies indicated. (echinobase.org)
  • For indirect immunofluorescence, cells were cultivated on coverslips, fixed in 3.7% formaldehyde in PBS for 7?min Sh3pxd2a at room heat, permeabilized with 0.5% Triton X-100 in PBS + 1% BSA for 6?min on snow and stained with the indicated antibodies. (mergullo.net)
  • Further, the increase in cell area was Rac1-dependent, and the iris extract led to slight Rac activation. (iucc.ac.il)
  • Compositions and methods for modulating the activation of nuclear factor κB (NF-κB) are provided. (justia.com)
  • The present invention relates generally to compositions and methods for modulating the activation of nuclear factor κB (NF-κB). (justia.com)
  • Double staining was performed to determine the apoptotic cells. (chemoprev.org)
  • Western Blot and TUNEL results suggested that Nigella sativa seed extracts regulated the expression of pro- and anti- apoptotic genes, indicating its possible development as a potential therapeutic agent for cervical cancer upon further investigation. (biomedcentral.com)
  • Biochemical verification of the extract based on activity-mediated fractionation and nuclear magnetic resonance analysis revealed that the active compounds belong to the group of iridals, a known group of triterpenoid. (iucc.ac.il)
  • BLM and hSSB1 also co-localised at nuclear foci following IR-induced double strand breaks and stalled replication forks. (biomedcentral.com)
  • We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. (cdc.gov)
  • Studies have explored the anticancer properties of different fruits, both in terms of their extracts and bioactive ingredients. (nature.com)
  • Embryonic stem cells (ESCs) are self-renewing, pluripotent cells derived from the inner cell mass (ICM) of a blastocyst [ 1 , 2 ]. (biomedcentral.com)
  • The purpose of this study was to investigate the expression pattern of Mage-D1 in rat tooth germ development and its differential mineralization ability to ectomesenchymal stem cells (EMSCs), and to explore its potential mechanism. (researchsquare.com)
  • Ectomesenchymal stem cells (EMSCs) derived from the cranial neural crest are significant in tooth development and dental mineralization. (researchsquare.com)
  • Inhibitor of RhoA kinase did not interfere with the ability of the iris extract to increase HeLa cell area. (iucc.ac.il)
  • Similar to the major α1 globin transcript, this upstream RNA is present almost exclusively in the cytoplasm of the transfected COS-7 cells. (tmu.edu.tw)
  • Like other members of the Rel family of transcriptional activators, NF-κB is sequestered in an inactive form in the cytoplasm of most cell types. (justia.com)
  • NF-κB in the cytoplasm of nonstimulated cells. (justia.com)
  • The survival rates of tumor patients are decreasing because of tumor cell resistance to anticancer chemotherapies [ 1 ]. (oncotarget.com)
  • These data are the first to demonstrate glia cell type-dependent B7-H1 regulation in vivo, resulting in adverse effects on antiviral CD8 T cell function. (jak-inhibitors.com)
  • In this study, we demonstrate that hSSB1 and BLM helicase form a complex in cells and the interaction is altered in response to ionising radiation (IR). (biomedcentral.com)
  • HeLa cell nuclear extracts (NEs) (lane 13) served as a control. (echinobase.org)
  • C) Extracts from embryos (5 hpf) in which indicated MOs were injected (lanes 2-8) or uninjected (lane 1) were subjected to 7.5% SDS-polyacrylamide gel electrophoresis. (echinobase.org)
  • HeLa cell NEs were also analyzed (lane 9) as a control. (echinobase.org)
  • To further study the role of tTG in liver disease, we initiated investigations into the effect of a proinflammatory mediator, tumor necrosis factor (TNF)-alpha, on tTG activity in cultured liver cells. (nih.gov)
  • Several members of the Epidermal Growth Factor Receptor (EGFR) family, have been identified in the nucleus and nucleolus of many cancer cells, but their function in these compartments remains unexplored. (biomedcentral.com)
  • Cell cycle examination Subconfluent cells were harvested and washed in 16phosphate buffered saline, subsequently fixed in icecold 75 ethanol and stained with propidium iodide. (wee1signaling.com)