Hela cell extracts. Medical search. Frequent questions

Tamarind bark dichloromethane extract was found to be cytotoxic to HeLa and PA-1 cells. (ijpsonline.com)
Extracts from hermaphrodite trees had a higher content of phenolic compounds, and exhibited higher antioxidant and cytotoxic activities. (ualg.pt)
Aida had the highest radical scavenging activity and total content of phenolics, Mulata the highest capacity to inhibit lipid oxidation and Gasparinha the strongest cytotoxic activity on HeLa cells. (ualg.pt)
Besides, this alkaloid is non-cytotoxic to RAW264.7 cells. (news-medical.net)
Steroidal alkaloids such as solamargine, solasodine, and solasonine have been detected in the fruit peels of Solanum melongena , which were cytotoxic to cancer cell lines such as HCT116, HepG2, HEp2, HeLa, and MCF7. (news-medical.net)
Ataluntum present in Atalantia monophylla fruit peels was a potent cytotoxic compound for cholangiocarcinoma cells, with a 4.7-fold greater activity than the standard ellipticine. (news-medical.net)
It has been demonstrated that BGP could enhance the cytotoxic effect of cytostatic drugs on tumor cells. (propolisscience.org)
It was cytotoxic to Syrian hamster embryo (SHE) cells and Chinese hamster V79 cells. (nih.gov)

HeLa (cervix adenocarcinoma) cells were grown to confluency in DMEM plus 10% fetal bovine serum. (neb.com)
Fatty acid profile and carotenoid content were characterized by gas chromatography and spectrophotometry techniques, while in vitro anticancer activity was assessed by MTT assay against cervix (HeLa), breast (T47D and MCF-7) and colon (WiDR) cancer cells. (kemdikbud.go.id)
The HeLa cell line was established from an adenocarcinoma of the cervix in 1952. (ipracell.be)

Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500. (genetex.com)

However, they are involved in multiple cell activities such as cell differentiation, apoptosis, cell growth and tumor suppression. (medscape.com)
Further in vitro assays demonstrated that the bioactive fraction was inducing caspase-9 mediated apoptosis in the cells and was able to reduce the total cell count of the cells as evidenced by flow cytometry analyses. (ijpsonline.com)
It can be concluded that the presence of cantharidin might be responsible for inhibition of proliferation and induction of apoptosis in the cancer cells. (ijpsonline.com)
We further identified that OTUD1 is involved not only in TNF-α-induced apoptosis and necroptosis but also in KEAP-1-mediated oxidative stress response and reactive oxygen species (ROS)-induced cell death, oxeiptosis. (nature.com)
1. Ginsenoside Rh2 Induces HeLa Apoptosis through Upregulating Endoplasmic Reticulum Stress-Related and Downstream Apoptotic Gene Expression. (nih.gov)
12. Trichomonas vaginalis induces apoptosis via ROS and ER stress response through ER-mitochondria crosstalk in SiHa cells. (nih.gov)
15. Novel small molecule inhibitor of Kpnβ1 induces cell cycle arrest and apoptosis in cancer cells. (nih.gov)
In the last years, a wide spectrum of phytochemicals have been found to be beneficial to health interacting with molecular signaling pathways underlying critical functions such as cell growth and differentiation, apoptosis, autophagy, inflammation, redox balance, cell volume regulation, metabolic homeostasis, and energy balance. (frontiersin.org)
The decrease in cell viability was associated with apoptosis on HeLa and MDA-MB-231 lines. (ualg.pt)

50.4 ± 2.6 μmol FeSO4 equivalent/g·DW and lowest IC50 values of 160.4 ± 11.6 μg/mL, 126.1 ± 10.8 μg/mL, and 178.3 ± 13.1 μg/mL against Hela, HepG-2 and U251 tumor cells, respectively. (unboundmedicine.com)
May contribute to the less invasive phenotypes of lepidic growth tumor cells. (cusabio.com)
Expression of these retrotransposons appears to be favored in cells of germ line origin, as well as in some other tumor cells of epithelial origin. (nih.gov)

In the first part of the presentation, two biochemical mutants were generated from HeLa nuclear extracts using a fractionation approach (e.g., ammonium sulfate precipitation and column chromatography), and each mutant was used as a receptor to isolate a nuclear activity capable of restoring MMR to the biochemical mutant in a functional in vitro MMR assay. (nih.gov)

Immunoprecipitation: MED23 Antibody [NB200-338] - Nuclear extract (5 mg) from HeLa cells. (novusbio.com)
Antibody used at 4 ug/5 mg extract. (novusbio.com)
Western blot analysis of extracts from various cell lines using GPX4 Antibody. (cellsignal.com)
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:500. (genetex.com)
HeLa cells stained with Actin (red) and also with our chicken polyclonal antibody to Vimentin, (green) (CH22108). (neuromics.com)
Western blot analysis of extracts from HeLa cells, using CADM1 antibody. (cusabio.com)
Untreated (-) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GTX128145) diluted at 1:2000. (genetex.com)

Further, bioassay guided fractionation of the extract was carried out using thin layer chromatography. (ijpsonline.com)
Bioassay guided fractionation indicated that the fourth fraction of the extract was the bioactive component. (ijpsonline.com)
This study provides evidence for the anticancer properties of A. californica when extracted in water and ethyl acetate, and supports the importance for further purification of the crude extracts and isolation of potential new anticancer compounds through bio-guided fractionation. (spandidos-publications.com)
For each plant part, three extract conditions were used, including water, EtOH and EtOAc, in order to correlate to traditionally used methods and compounds with different polarities as a starting point for future bio-guided fractionation. (spandidos-publications.com)

Plant parts and extract conditions of A. californica and their cytotoxicity against three human cancer cell lines in vitro. (spandidos-publications.com)
Other benzoyl tyramines from A. monophylla displayed cytotoxicity towards HeLa, HCT116, and MCF-7 cell lines. (news-medical.net)
Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo. (cusabio.com)

Human female HeLa genomic DNA. (neb.com)
Genomic DNA was isolated by a standard genomic purification protocol (1), phenol extracted and equilibrated to 10 mM TrisHCl (pH 7.5) and 1 mM EDTA. (neb.com)

8. Exogenous interleukin-1 beta promotes the proliferation and migration of HeLa cells via the MEK/ERK signaling pathway. (nih.gov)
One study extracted flavones from Wisteria floribunda fruit peels and found several promising candidates for the inhibition of platelet-derived growth factor (PDGF)-induced proliferation of vascular smooth muscle cells (VSMCs). (news-medical.net)
The Ras family of GTPases is a collection of molecular switches that link receptors on the plasma membrane to signaling pathways that regulate cell proliferation and differentiation. (embl.de)

Tamarind bark extract was prepared with dichloromethane as the solvent using Soxhlet apparatus and treated on HeLa and PA-1 cell lines to determine the anticancer potential by 3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay. (ijpsonline.com)
To comprehensively explore the DUBs involved in LUBAC-mediated NF-κB activation, we prepared 88 human DUB cDNAs and analyzed their effects by a luciferase assay in HEK293T cells (Fig. 1a ). (nature.com)

Western blot analysis of extracts from HeLa and MCF7 cells using Prdx1 (D5G12) Rabbit mAb. (cellsignal.com)

Ultrasonic-assisted enzymatic extraction (UAEE) is confirmed as a novel extraction technology with main advantages of enhancing extraction yield and physiological activities of the extracts from various plants. (unboundmedicine.com)
The results indicated that the UAEE was an efficient alternative to improve extraction yield and enhance the antioxidant and antitumor activities of the extracts. (unboundmedicine.com)
Gender and cultivar significantly influenced the chemical content and the biological activities of the extracts. (ualg.pt)

Thus, OTUD1 is a crucial regulator for the inflammatory, innate immune, and oxidative stress responses and ROS-associated cell death pathways. (nature.com)
16. Extracts from Pulsatilla patens target cancer -related signaling pathways in HeLa cells. (nih.gov)

HeLa cells were cultured in MEM alpha medium supplemented with 10% FBS. (nih.gov)
Jurkat cells (3.2×10 5 cells/mL) were suspended in RPMI medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin, plated on a 35cm dish, and incubated overnight at 37˚C in a 5% CO 2 atmosphere. (dojindo.com)

Publications are still being populated for the years 2016, 2017, and 2018 and the number of publications using HeLa cells per year are lower as a result. (nih.gov)

The course features lectures from world-class genetics and genomics experts and then immerses students in the lab for days of hands-on training in the latest ways to extract data from DNA, RNA, and mitochondria. (nih.gov)

Although the mutation generates a stop codon, a small amount of internally deleted, but functional, dystrophin protein is produced in the patient cells. (nature.com)
Therefore, we searched for small chemicals that enhance exon skipping, and found that TG003 promotes the skipping of exon 31 in the endogenous dystrophin gene in a dose-dependent manner and increases the production of the dystrophin protein in the patient's cells. (nature.com)
PML is a NB protein associated with Sp100 [ 19 ] and originally identified as a protein aberrantly expressed in leukemic cells of patients with acute promyelocytic leukemia. (medscape.com)
[ 37 ] PML protein appears to be a transformation and cell-growth suppressor with transcription regulatory functions. (medscape.com)
Immunoprecipitation of Phospho-Prdx1 (Tyr194) protein from A-431 cells treated with EGF (50 ng/ml, 15 min), using Rabbit (DA1E) mAb XP ® Isotype control #3900 (lane 2) or Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit mAb (lane 3). (cellsignal.com)
Multiphoton fluorescence image of cultured HeLa cells with a fluorescent protein targeted to the Golgi apparatus (orange), microtubules (green) and counterstained for DNA (cyan). (nih.gov)
7. Trehalose decreases mRNA and protein expressions of c- jun and jun B in human cervical cancer HeLa cells. (nih.gov)
19. Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells. (nih.gov)
The use of plasmids requires the target protein to be stably expressed, while small fluorescent molecules are widely used because they can simply be added to cells. (dojindo.com)
However, its abundance in the cell is very low since only 1 lOpg of pure protein could be recovered from 150 grams of cultured cells. (open.ac.uk)
This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. (genetex.com)
Full length human recombinant protein of human AK4 produced in HEK293T cell. (thermofisher.com)
Values represent average percentage protein abundance relative to uninduced cells standard deviation. (icem2012.org)
The protein expression levels of p-IKKα/β, NF-κB and COX-2 were upregulated by PpIX-PDT but significantly attenuated when in combination with BGP extract. (propolisscience.org)
IF2 has a conserved GTP-binding notype of a fun12 yeast strain and purified hIF2 substituted domain near the center of the protein, and GTP hydrolysis is for yIF2 to stimulate the translational activity of extracts from required for IF2 release after subunit joining (3, 4). (nih.gov)
Parental A549 and A549 cells stably expressing Npro of bovine viral diarrhea virus or the V protein of simian virus 5 (C), as well as (D) human skin fibroblast control cultures or with a defect in STAT2 infected with BRBV (MOI 0.001). (cdc.gov)
The members of the DNA Repair Interest Group perform research in areas including DNA repair enzymology and fine structure, mutagenesis, gene and cell cycle regulation, protein structure, and human disease. (nih.gov)

It is concluded that altering the growth conditions of D. viridis can lead to higher cell densities and higher lipids content which can be exploited for biofuel production. (scielo.br)
Three different extract conditions (aqueous, EtOH and EtOAc) of four different parts (bracts, leaves, roots and stems) of the plant Anemopsis californica (A. californica) were evaluated for their effect on the growth and migration of human colon cancer cells, HCT-8, and the breast cancer cell lines Hs 578T and MCF-7/AZ. (spandidos-publications.com)
Furthermore, significant growth inhibitory activity against the three cell lines was found for the ethyl acetate extract of the roots, while the aqueous extract of the roots influenced the migratory capacity of the three cell lines. (spandidos-publications.com)
Thus, this study evaluated the effects of extracts obtained from different plant parts (bracts, leaves, roots and stems) on the growth and migration of human cancer cell lines, including HCT-8 colon, mammary estrogen-independent Hs 578T and estrogen-dependent MCF-7/AZ cells. (spandidos-publications.com)
When fed to laboratory animals that had cancer cells implanted in them, amygdalin did not reduce the tumor size or slow their growth. (mskcc.org)
In addition to its association with Ras, GAP has been shown to bind to several tyrosine-phosphorylated proteins in cells stimulated by growth factors or expressing transforming tyrosine kinase variants. (embl.de)

Whole RNA was extracted and IFN-β and actin transcripts detected by real-time reverse transcription PCR. (cdc.gov)

However, these dyes have some limitations, including short-term retention in cells, decreased fluorescence intensity in serum, and high background. (dojindo.com)
The fluorescence intensity of 50C100 cells for each time point was decided using ImageJ and the average fluorescence intensity in EXOC6 knockdown cells expressed as a percentage of the corresponding value in the scrambled siRNA-treated group (Scr). (icem2012.org)

ABSTRACT This study was designed to examine the chemical composition and in vitro antioxidant activity of the hydrodistillated essential oil and various extracts obtained from Premna integrifolia Linn. (scielo.br)
Our findings provide evidence for a synergistic effect of BGP extract in PpIX-PDT both in vitro and in vivo. (propolisscience.org)

Indeed, a hereditable non-polyposis colorectal cancer cell line (HCT116) demonstrated lower dI repair activity that was partially attributed to lack of mismatch repair. (biomedcentral.com)

This presentation reports our recent efforts to understand the molecular mechanisms of MMR in human cells and includes two lines of work: (i) identification and characterization of MMR components, and (ii) reconstitution of the human MMR reaction in a defined system. (nih.gov)

To assess the synergistic effect of BGP extract on protoporphyrin IX (PpIX)-mediated photocytotoxicity, MTT assays were performed using A431 and HeLa cells. (propolisscience.org)
Western blotting analysis was performed to examine the pro-apoptotic proteins, anti-apoptotic proteins and inflammation related proteins in A431 cells. (propolisscience.org)
Our findings reveal that BGP extract increased PpIX-mediated photocytotoxicity in A431 and HeLa cells. (propolisscience.org)
BGP extract was also found to significantly enhance the intracellular accumulation of PpIX in A431 cells. (propolisscience.org)

Scanning electron micrograph of an apoptotic HeLa cell using a Zeiss Merlin HR-SEM. (nih.gov)

The number and size of the NB in the cell increase in response to stimuli, including interferons, heat-shock proteins and viral infections. (medscape.com)
The molecular weight of all six proteins is 42kDa, and one or more actins is found in essentially every type of crude cellular and tissue extract. (neuromics.com)
The accessory GTPase-activating proteins (GAPs) negatively regulate the cell signaling by increasing the slow intrinsic GTP to GDP hydrolysis rate of Ras. (embl.de)
Important modulators of NF-κB activation are the inhibitor proteins IκBα and IκBβ (referred to herein as IκB), which associate with (and thereby inactivate) NF-κB in the cytoplasm of nonstimulated cells. (justia.com)
Cell 75:495, 1993), and a series of unique E6-AP homologous proteins have been identified (Huibregtse et al. (justia.com)
Another type of DNA segment, the so-called Alu sequence, also inserts in new positions in human chromo- somes, but Alu segments do not appear to encode en- zymes or other proteins that are expected to be required for their own transposition. (nih.gov)
genes or proteins) that are substantially more constrained than extracting factual assertions. (nih.gov)

Antioxidant activities of the essential oil and organic extracts of chloroform, ethyl acetate and methanol were determined by three different test systems namely DPPH (2,2-diphenyl-1-picrylhydrazyl), superoxide and nitric oxide radical scavenging assays. (scielo.br)
Our results demonstrated that for each of the cell lines tested, the majority of ethyl acetate extracts of all the plant parts are more toxic than the aqueous and ethanol extracts. (spandidos-publications.com)

Like other members of the Rel family of transcriptional activators, NF-κB is sequestered in an inactive form in the cytoplasm of most cell types. (justia.com)

This study aimed to evaluate the antioxidant and anti-cancer activities of C. chinense flower ethanolic extract and identify the bioactive compounds. (researchgate.net)
High-performance liquid chromatography was used to identify and quantify the bioactive compounds of the extract. (researchgate.net)
To study this, we explored different methods to simultaneously extract and separate compounds from Nigella sativa L and performed antioxidant tests ( β -carotene and DPPH) for all collected fractions. (hindawi.com)
We found that only two solvents (hexane, acetone) of different polarities could easily extract and simultaneously separate the components of Nigella sativa L. The antioxidant fractions that we collected had close activity to reference compounds but were more active than the corresponding mother extracts. (hindawi.com)
Most previous studies explored the compounds hot- or cold-extracted with polar or nonpolar solvents as well as the essential oil [ 15 , 16 ]. (hindawi.com)
Extracts from fruit pulps of six female cultivars and two hermaphrodite Portuguese carob trees [(Ceratonia siliqua L., Fabaceae)] exhibited strong antioxidant activity and were rich in phenolic compounds. (ualg.pt)
Catechin and gallic acid (GA) were the main compounds identified in the extracts, and GA contributed to the antioxidant activity. (ualg.pt)
In the present review, researchers discussed the biological compounds present in fruit peel extracts and their potential applications. (news-medical.net)

Cells grown to ~90% confluent were used for RNA-seq. (nih.gov)
HeLa cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×10 6 cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. (ipracell.be)
It can be grown on any of these cell lines treated with cycloheximide: McCoy, HEp-2, HeLa, or buffalo green monkey cells. (medscape.com)

In Escherichia coli , deoxyinosine (hypoxanthine deoxyribonucleotide, dI) is removed through an alternative excision repair pathway initiated by endonuclease V. However, the correction of dI in mammalian cells appears more complex and was not fully understood. (biomedcentral.com)
In mammalian cells, base excision repair (BER) was thought to be the major pathway for dI repair. (biomedcentral.com)
Nfi homologues from Thermotoga maritima possess 3′-exonuclease activity that might be used for removal of damaged bases [ 14 ], but similar exonuclease activities were not found in EndoV from E. coli and mammalian cells. (biomedcentral.com)
High-efficiency transformation of mammalian cells by plasmid DNA. (nih.gov)
Several major species of E2 enzymes have been characterized in mammalian cells, plants, and yeast. (justia.com)

Cytolytic Effects of Mumps Virus in Tissue Cultures of Epithelial Cells. (nih.gov)
Epithelial cells can be taken from the eye, urethra (remove purulent exudate), endocervix (remove purulent exudate), and rectum. (medscape.com)
Obtain specimen from epithelial cells in the infected site. (medscape.com)
2] Its host cells are primarily epithelial cells, which line the conjunctiva, respiratory tract, urogenital tract, and rectum. (medscape.com)

Interestingly, although mTid-1 is found tyrosine-phosphorylated in v-src-transformed fibroblast cells, GAP selectively binds to the unphosphorylated form of mTid-1. (embl.de)

Nigella sativa L was hot-extracted by Soxhlet and mother extracts and was separated using silica column chromatography with adequate eluents. (hindawi.com)

The blue stain reveals DNA in the nuclei of these cells. (neuromics.com)
Isolated nuclei (HeLaS3) or whole cells (germ cells) were sonicated using a Diagenode Bioruptor to an average size of 200 bp. (nih.gov)

At 5.0 mM sodium nitrate, by using 20 g L-1 of glucose in mixotrophic culture of D. viridis, cell number augmented by 36.4 percent compared to the cultures with no added glucose. (scielo.br)

New NIH policy requires researchers to apply for access to the full genome sequence data from HeLa cells. (nih.gov)
The National Institutes of Health today announced in Nature that it has reached an understanding with the family of the late Henrietta Lacks to allow biomedical researchers controlled access to the whole genome data of cells derived from her tumor, commonly known as HeLa cells. (nih.gov)
Access to the whole genome data of these cells will be a valuable reference tool for researchers using HeLa cells in their research. (nih.gov)
The HeLa genome is another chapter to the never ending story of our Henrietta Lacks," said Lacks family spokesperson and Henrietta's granddaughter Jeri Lacks Whye. (nih.gov)
In the same issue of Nature, a team headed by Jay Shendure, M.D., Ph.D., of the University of Washington, Seattle, published a whole genome analysis of the CCL-2 strain of HeLa cells. (nih.gov)
That paper, which acknowledges the contributions of the Lacks family, pieced together the complex, rearranged genome of the HeLa cells and identified an insertion of the human papilloma virus near an oncogene that may explain the aggressiveness of Lacks' cancer. (nih.gov)
Earlier this year, researchers in Germany published a scientific paper that described the first sequence of the full HeLa genome, comparing the DNA of HeLa cell lines with that of cells from healthy human tissues. (nih.gov)
The sequencing and posting of the HeLa genome brought into sharp relief important ethical and policy issues," said Dr. Collins. (nih.gov)
The new controlled access policy for full genome sequence data from HeLa cells will give the Lacks family the ability to have a role in work being done with the HeLa genome sequences and track any resulting discoveries. (nih.gov)

The selected key independent variables were cellulase concentration (X1: 1.5%-2.5%), extraction time (X2: 20-30 min) and extraction temperature (X3: 40-60 °C). The optimal extraction conditions for total phenolic content (TPC) value of the extracts were determined as 1.74% cellulase concentration, 25.5 min ultrasonic extraction time and 49.0 °C ultrasonic temperature. (unboundmedicine.com)
EDGAR (Extraction of Drugs, Genes and Relations) is a natural language processing system that extracts information about drugs and genes relevant to cancer from the biomedical literature. (nih.gov)
In this paper, we report a system, EDGAR (Extraction of Drugs, Genes and Relations), designed to extract factual information from the MEDLINE database on the relationships between genes, drugs and cells. (nih.gov)

Artificial antioxidants such as butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) are commonly reported for their efficiency in delaying cell deterioration but are also suspected to have negative health effects like carcinogenesis and toxicity [ 1 ]. (hindawi.com)

4. Dickkopf-1 expression is repressed by oncogenic human papillomaviruses (HPVs) and regulates the Cisplatin sensitivity of HPV-positive cancer cells in a JNK-dependent manner. (nih.gov)
Results of sensitivity testing of BRBV to type I IFN-induced antiviral state in cell culture. (cdc.gov)

Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. (genetex.com)
Changes in IFN-β transcripts were calculated in comparison to mock-treated cells. (cdc.gov)

These DNA lesions were embedded at the In or Out rotational settings near the dyad axis in nucleosome core particles reconstituted either with native histones extracted from HeLa cells (HeLa-NCP) or with recombinant histones (Rec-NCP). (nih.gov)

Cell Biol. (dojindo.com)
J Cell Biol (2005) 168 (1): 41-54. (rupress.org)

Total lipids expressed as µg mL-1 of culture also increased with increase in nitrate concentration up to 5.0 mM sodium nitrate, whereas when expressed on the per cell basis, total lipids stayed relatively constant at most of the tested nitrate concentrations except at 0.5 mM which was 31.4 percent higher compared to 1.0 mM nitrate. (scielo.br)
At concentration of 25 ppm, the extract was able to kill all the 4 cells tested. (kemdikbud.go.id)

These are multiprotein complexes that have been observed in all studied mammalian cell lines. (medscape.com)
The aim of the current study was to evaluate the anticancer potential of tamarind bark and its bioactive fraction on HeLa and PA-1 cell lines. (ijpsonline.com)
Western blot analysis of extracts from various cell lines using TXNIP (D5F3E) Rabbit mAb. (cellsignal.com)
HeLa cells are the most widely used human cell lines in existence today. (nih.gov)
The extracts decreased the viability of different human cancer cell lines on a dose- and time-dependent manner. (ualg.pt)
A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. (nih.gov)

Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly. (cusabio.com)

The phenolic extracts from the stems of T. quadrispinosa had significant antioxidant and antitumor activities, which could be used as a source of potential antioxidant and antitumor agents. (unboundmedicine.com)

A side experiment showed that in fatty HeLa cells that expressed alcohol dehydrogenase, there was more triglyceride even in the absence of added alcohol, and if alcohol were present in the medium, the fat accumulated. (nih.gov)

18. MTA2 silencing attenuates the metastatic potential of cervical cancer cells by inhibiting ap1 -mediated MMP12 expression via the ASK1/MEK3/p38/YB1 axis. (nih.gov)
Our aim was to identify potential anticancer activity in crude A. californica extracts, given that this plant is used by Native Americans to treat a variety of diseases, including cancer. (spandidos-publications.com)
Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
Promoters claimed that the cyanide released from amygdalin selectively killed cancer cells, leaving normal tissue cells unharmed. (mskcc.org)
Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. (cusabio.com)
miR-21 enhances chemo-resistance in tongue cancer cells via directly targeting CADM1, and an inverse correlation between miR-21 and CADM1 expression in vivo. (cusabio.com)
This automatically extracted information has remarkable potential to facilitate computational analysis in the molecular biology of cancer, and the technology is straightforwardly generalizable to many areas of biomedicine. (nih.gov)
1999]. Our goal in this work is to extract factual assertions, in the form of first order predicate calculus statements, about the relationships between genes and drugs in cancer therapy. (nih.gov)

Photodynamic therapy (PDT) is a therapeutic approach used against malignant cells. (propolisscience.org)

Panel B: HeLa cells treated with either scrambled (Scr) or EXOC6 siRNA smartpools were examined for fluorescent transferrin uptake after exposure of cells to fluorescent transferrin for the times indicated. (icem2012.org)

Bisphenol AF also induced metaphase arrest and micronuclei in V79 cells. (nih.gov)

Western blot of crude extract of the human carcinoma HeLa cell line. (neuromics.com)

Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. (nih.gov)

May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. (cusabio.com)

Fresh RPMI medium was poured into the dish and cells were passaged and analyzed every 2 days by flow cytometry. (dojindo.com)

The four BPDE-dG adduct samples are excised with different efficiencies in free DNA, but in HeLa-NCPs, the efficiencies are reduced by a common factor of 2.2 ± 0.2 relative to the NER efficiencies in free DNA. (nih.gov)

PpIX-PDT with BGP extract treatment resulted in a decrease in Bcl-xL and an increase in NOXA, Bax and caspase-3 cleavage. (propolisscience.org)

The extracts from the stems of T. quadrispinosa were characterized by determination of their antioxidant activities through 2,2-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS), 1,1-Diphenyl-2-picrylhydrazxyl (DPPH) radical scavenging, total antioxidant capacity (TAC), ferric reducing antioxidant capacity (FRAC) methods and of their antitumor activity by MTT method. (unboundmedicine.com)
HDH VII possesses the highest specific activity among all the helicases, so far, extracted and characterized from HeLa cells, and this activity is further stimulated nearly a hundred-fold by hRPA. (open.ac.uk)
1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)

Our HeLa cell pellets are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin. (ipracell.be)

Considering the richness of its biological heritage, it is possible that the seed extracts could contain one or more active ingredients that specifically target each disease. (hindawi.com)
A recent study published in Current Issues in Molecular Biology reviewed the potential biological applications of fruit peel extracts. (news-medical.net)
RNAi cells 24 and 36h Actarit post induction with biological function as assigned in Actarit GO:term and cellular localisation as assigned in GO:terms or predicted by SignalP [91] and TMHMM [89]. (icem2012.org)

Western blot analysis of extracts from A-431 cells, untreated or treated with EGF (50 ng/ml, 15 min), λ phosphatase (13333 Unit/ml, overnight) using Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit mAb (upper) and Prdx1 (D5G12) Rabbit mAb #8499 (lower). (cellsignal.com)
Western blot analysis of extracts from 293 and THP-1 cells using GPX1 (C8C4) Rabbit mAb. (cellsignal.com)
The highest yield of total polyphenols at saturation was 53.76 mg gallic acid equivalents per gram of dry weight, achieved at 60 °C. The analysis of this extract using liquid chromatography-diode array-mass spectroscopy led to the tentative identification of gallic acid, myricetin 3-O-rhamnoside, quercetin 3-O-rhamnoside and quercetin p-hydroxy benzoyl 3-O-rhamnoside. (researchgate.net)
Worksheets D and E: SILAC analysis of whole cell proteome at 24 and 36 hours knockdown of Sec15. (icem2012.org)

Anatomy9

Organisms6

Chemicals and Drugs33

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes31

Information Science3

Health Care1

Cytotoxic8

Cervix3

HepG21

Apoptosis9

Tumor cells3

Nuclear extracts1

Antibody7

Fractionation4

Cytotoxicity3

Genomic2

Proliferation3

Assay2

MCF71

Activities of the extracts3

Pathways2

Medium supplemented with 102

20181

Mitochondria1

Protein17

Growth6

Actin1

Fluorescence2

Vitro2

HCT1161

Characterization1

A4314

Apoptotic1

Proteins7

Ethyl acetate2

Cytoplasm1

Compounds8

Grown3

Mammalian cells5

Epithelial cells4

Fibroblast Cells1

Soxhlet1

Nuclei2

Cultures1

Genome9

Extraction3

Reported for their efficiency1

Sensitivity2

Mock2

Recombinant1

Biol2

Concentration2

Lines6

Molecule1

Phenolic1

Fatty1

Cancer8

Malignant1

Fluorescent1

Arrest1

Crude1

Sequences1

Differentiation1

Flow cytometry1

Samples1

Decrease1

Activity3

Purification1

Biological3

Analysis4