SILAC RPMI is formulated without L-arginine and L-lysine for multiple isotopic amino acid labeling and has no effect on cell morphology or growth rates. (capricorn-scientific.com)
Biochemical processes commonly observed in the biosphere, including the decarboxylation and deamination of amino acids, are the key factors in this isotopic approach. (springeropen.com)
We also summarize two recently examined topics with new datasets: the isotopic compositions of individual biosynthetic products (chlorophylls and amino acids) and those of animal organs for further pursuing the basis of the methodology. (springeropen.com)
The isotopic distributions of carbon and nitrogen in a single organism as well as in the whole biosphere are strongly regulated, so that their major components such as amino acids are coordinated appropriately rather than controlled separately. (springeropen.com)
Although the carbon and nitrogen isotopic compositions of diverse types of organic matter were measured and discussed by the pioneers of isotope geochemistry (e.g. (springeropen.com)
As our knowledge of the distributions of carbon and nitrogen isotopes in organic matter and the factors controlling them has accumulated, the scope of isotopic measurements has greatly expanded. (springeropen.com)
Synthesis7
As the heaviest essential mineral nutrient , iodine is required for the synthesis of thyroid hormones . (wikipedia.org)
As described in his autobiography, My life in Science , Brenner thought about how genetic information guides protein synthesis even before he saw the double helix model in April 1953 at Cambridge. (ucdavis.edu)
With the discovery that protein synthesis occurs at ribosomes, it was largely assumed that the intermediate was the ribosomal RNA (rRNA). (ucdavis.edu)
Another concern was what Brenner called the "paradox of the prodigious rate of protein synthesis. (ucdavis.edu)
If rRNA is the intermediate for protein synthesis, a significant increase of new rRNA should be observed. (ucdavis.edu)
These results ruled out the possibility of any stable intermediate like rRNA because if the intermediate were stable, galactosidase synthesis should have continued for a while after the gene was removed. (ucdavis.edu)
The next step was to plan experiments testing whether the short-lived RNA was the intermediate messenger that guides protein synthesis. (ucdavis.edu)
Processes3
SILAC labeling is accomplished via normal metabolic processes (e.g., cell division), by incorporating non-radioactive stable amino acid isotopes into newly synthesized proteins. (capricorn-scientific.com)
Chlorophylls provide information about the particular processes of various photoautotrophs, whereas amino acids provide a precise measure of the trophic positions of heterotrophs. (springeropen.com)
In recent decades, the natural abundances of stable carbon and nitrogen isotopes (represented as 13 C/ 12 C and 15 N/ 14 N ratios, respectively) have been widely used to probe biogeochemical and ecological processes. (springeropen.com)
The RNA intermediate experiment could use this approach to label bacterial ribosomes with isotopes before phage infection and resuspended in the medium without isotopes right after infection, which would make the old ribosomes heavier than the new ribosomes and therefore distinguishable by density gradient centrifugation. (ucdavis.edu)