Hct116 cells from oxidative stress. Medical search. Frequent questions

Over lifetime, due to the action of several stressors such as DNA damage and telomere shortening, senescent cells accumulate in the organism and release a variety of pro-inflammatory cytokines responsible for low-grade inflammation. (frontiersin.org)
Gliadin proteins from these varieties were tested on human colon cancer cell line HCT116 to assess their effect on inflammation, oxidative and nitrosative stress, pro-inflammatory cytokines. (edu.in)
These include regulation of cell cycle, activation of mitochondrial pathway, induction of mitochondrial outer membrane permeabilization, induction of apoptosis, modulation of oxidative stress, amelioration of inflammation, modulation of insulin signaling and inhibition of angiogenesis. (mdpi.com)

Crocin, the main active saffron constituent, mitigates dichlorvos-induced oxidative stress and apoptosis in HCT-116 cells. (sciendo.com)

New anticancer therapy strategies refer to the inhibition of CDK-cyclin complexes as an important target to prevent uncontrolled proliferation and induce apoptosis in cancer cells ( 2 ). (spandidos-publications.com)
Natural polyamines, putrescine, spermidine and spermine, play essential roles in the regulation of cell growth and proliferation. (spandidos-publications.com)
The cell cycle analysis showed a significant inhibition of the HCT 116 cells proliferation following FBX-EMLs treatment compared to all the other conditions, with a higher number of cells accumulating on G2/M and pre-G1 phases, paralleled by a significant reduction of cells in G0/G1 and S phases. (carusogiuseppe.net)
Cell morphology studies were conducted to observe morphological changes, and the wound-healing assay was performed to evaluate the proliferation of honey-treated OSCC cells. (jcpjournal.org)
Sangju honey reduced cell viability, induced morphological changes, and significantly suppressed the proliferation and migration of Ca9-22 and YD-10B cells. (jcpjournal.org)
Sangju honey inhibits Ca9-22 and YD-10B cell proliferation by regulating EMT, inducing apoptosis, and suppressing the MAPK signaling pathway. (jcpjournal.org)
Many physiological processes, including proper tissue development and homeostasis, require a balance between apoptosis and cell 123653-11-2 manufacture proliferation. (bioinf.org)
Apoptosis and cell proliferation are linked by cell-cycle regulators and apoptotic stimuli that affect both processes. (bioinf.org)
A failure in regulating proliferation together with suppression of apoptosis are the 123653-11-2 manufacture minimal requirements for a cell to become cancerous [4]. (bioinf.org)
Two proteins involved intimately in regulating cell proliferation are Akt and the tumor suppressor p53 (p53). (bioinf.org)
p53 has an important protective role against undesired cell proliferation. (bioinf.org)
Data obtained, shows that IR-SF inhibited cell proliferation and induced typical apoptotic changes in the overall morphology of all the CRC cell lines tested. (afpm.org.my)
Hsieh SL, Li JH, Dong CD, Chen CW, Wu CC*. Carnosine suppresses human colorectal cancer cell proliferation by inducing necroptosis and autophagy, and reducing angiogenesis. (nutr-metab-pu.org)
In this study, we examined the effects of quercetin on cell cycle, viability, and proliferation of cancer cells, either singly or in combination with the microtubule-targeting drugs taxol and nocodazole. (vdocuments.net)
However, prolonged exposure of the cells to the highest protective dose of quercetin was still able to pre- vent cell proliferation. (vdocuments.net)
Therefore, this study proves that Rh4 inhibits cancer cell proliferation by activating the ROS/p53 signaling pathway and activating autophagy to induce ferroptosis, which provides necessary scientific evidence of the great anticancer potential of Rh4. (hindawi.com)

Various physiological and pathological conditions such as glucose starvation, inhibition of protein glycosylation and oxidative stress may cause an accumulation of unfolded proteins in the endoplasmic reticulum (ER), leading to the unfolded protein response (UPR) and autophagy. (spandidos-publications.com)
CF possesses antioxidant properties which protect erythrocytes, lymphocytes, and biomolecules against free radical attacks, suggesting that it may be an adjuvant intervention in the prevention and treatment of various physiological and pathological conditions related to oxidative stress [1]. (bioinf.org)

Arising from the transformation of epithelial cells in the colon and/or rectum into malignant cells, the foundation of CRC pathogenesis lies in the progressive accumulation of mutations in oncogenes and tumor-suppressor genes, such as APC and KRAS. (afpm.org.my)

18 ]. They reported that the expression of Prx1 and Prx6 is selectively regulated in alveolar type II cells isolated from vitamin E-depleted rats, depending on the severity of oxidative load. (biomedcentral.com)
2012). Crocin "saffron" protects against beryllium chloride toxicity in rats through diminution of oxidative stress and enhancing gene expression of antioxidant enzymes. (sciendo.com)
Protective effect of aqueous saffron extract (Crocus sativus L.) and crocin, its active constituent, on renal ischemia-reperfusion-induced oxidative damage in rats. (sciendo.com)
11. Bargi R, Asgharzadeh F, Beheshti F, Hosseini M, Sadeghnia HR, Khazaei M. The effects of thymoquinone on hippocampal cytokine level, brain oxidative stress status and memory deficits induced by lipopolysaccharide in rats. (ac.ir)
Anwer, T. et al: Protective effect of Withania somnifera against oxidative stress and pancreatic beta-cell damage in type 2 diabetic rats. (natur-wiki.de)

Indeed, inhibition of the HSP60 release or HSP60-IKK interaction sensitizes the cancer cells to mild oxidative stress and regresses the tumorigenic growth of cancer cells in the mouse xenograft model. (ewha.ac.kr)
Increased manifestation levels of p53, p21, and p27, downregulation of c-myc and Bcl-2, and inhibition of Akt activation were also found in CF-treated MSTO-211 and HCT-116 cells. (bioinf.org)
Tioconazole and Chloroquine Act Synergistically to Combat Doxorubicin-Induced Toxicity via Inactivation of PI3K/AKT/mTOR Signaling Mediated ROS-Dependent Apoptosis and Autophagic Flux Inhibition in MCF-7 Breast Cancer Cells. (lsbu.ac.uk)
Activation of TRPV1 by capsaicin caused significant inhibition of cancer cell growth and induced apoptosis and necrosis. (dovepress.com)

Therefore, we aimed to demonstrate the potential anticancer effects of Sangju honey on human oral squamous cell carcinoma (OSCC), particularly focusing on epithelial-mesenchymal transition (EMT) and apoptotic and mitogen-activated protein kinase (MAPK) signaling pathways. (jcpjournal.org)

Purvalanol, a novel cyclin-dependent kinase inhibitor, is referred to as a strong apoptotic inducer which causes cell cycle arrest in various cancer cells such as prostate, breast and colon cancer cell lines. (spandidos-publications.com)
In the present study, our aim was to determine the time-dependent, ER-mediated apoptotic and autophagy induction of purvalanol in HCT 116 colon cancer cells. (spandidos-publications.com)
HCT 116 colon cancer cells were exposed to purvalanol, which activated ER stress via upregulation of PERK, IRE1α gene expression, eIF-2α phosphorylation and ATF-6 cleavage at early time-points in the HCT 116 colon cancer cells. (spandidos-publications.com)
Although purvalanol-induced cell cycle arrest and apoptotic cell death were demonstrated in prostate ( 5 ), breast ( 6 ) and colon cancer cells ( 7 ), the exact molecular mechanism of purvanol-induced apoptosis has not been elucidated yet. (spandidos-publications.com)
10 TRPV1 agonist, capsaicin, is capable of inducing apoptosis 11 , 12 and inhibiting cancer cell growth by cell cycle arrest in many different types of cancer, for example, osteosarcoma, colon, and pancreatic cancer cells, while normal cells remained unharmed. (dovepress.com)

Saffron suppresses oxidative stress in DMBA-induced skin carcinoma: A histopathological study. (sciendo.com)
Ping TN, Hsieh SL, Wang JJ, Chen JB, Wu CC*. Panax notoginseng suppresses bone morphogenetic protein-2 expression in EA.hy926 endothelial cells by inhibiting the noncanonical NF-κB and Wnt/β-catenin signaling pathways. (nutr-metab-pu.org)
Hsieh SL, Hsieh S, Lai PY, Wang JJ, Li CC, Wu CC*. Carnosine suppresses human colorectal cell migration and intravasation by regulating EMT and MMP expression. (nutr-metab-pu.org)
Kakar, S. S. et al: Withaferin a alone and in combination with cisplatin suppresses growth and metastasis of ovarian cancer by targeting putative cancer stem cells. (natur-wiki.de)

Fifteen micromoles of purvalanol induced a reduction in cell viability by 20 and 35% within 24 and 48 h, respectively. (spandidos-publications.com)
Ca9-22 and YD-10B human OSCC cells were treated with 0.25% or 0.5% Sangju honey, and the cell viability was examined using the Cell Counting Kit-8 assay. (jcpjournal.org)
LCH significantly inhibited cell viability and colony growth in both Ox-sensitive and Ox-resistant CRC cells. (biomolther.org)
Quercetin also partially restored drug-induced loss in viability of treated cells for up to 72 h. (vdocuments.net)

Saffron ethanolic extract attenuates oxidative stress, spatial learning, and memory impairments induced by local injection of ethidium bromide. (sciendo.com)
The hepatocytes, endothelial and immune cells of the liver are aligned along this vasculature, and their spatial distribution along the liver zonation dictates their phenotypes and functions. (encyclopedia.pub)
This review discusses the current technologies for spatial transcriptomics ranging from the methods combining mechanical dissociation and single-cell RNA sequencing to computational spatial re-mapping. (molcells.org)
Here the authors use innovative approaches to combine spatial profiling with single cell transcriptomics to define tumor-proximal populations of fibroblasts that predict clinical outcome. (elifesciences.org)

Moreover, we determined that during purvalanol-mediated ER stress, autophagic machinery was also activated prior to apoptotic cell death finalization. (spandidos-publications.com)
Olomoucine, roscovitine and purvalanol are examples of CDK inhibitors (CDKIs) designed and investigated for their apoptotic potential on cancer cells ( 3 ). (spandidos-publications.com)
Τheir impact on the apoptotic cell death mechanism requires further elucidation. (spandidos-publications.com)
1996). Resistance to apoptotic cell death in a drug resistant T cell leukaemia cell line. (sciendo.com)
confirmed an apoptotic death for both cell lines. (bioinf.org)
CF treatment on leukemia cell lines induces cell death due to apoptotic mechanisms and altering 123653-11-2 manufacture cell metabolism through HIF-1 and GLUT-1 regulation [3]. (bioinf.org)
Altogether, the results of the present study demonstrate that IR-SF may induce apoptosis in human colorectal carcinoma cells through activation of ROS-mediated apoptotic pathways. (afpm.org.my)

To determine the antiproliferative effect of gamma-tocotrienol (GTT) treatment on differential protein expression in HepG2 cells. (biomedcentral.com)
HepG2 cells were treated with 70 μM GTT for 48 hours and differentially expressed protein spots were determined by two-dimensional electrophoresis (2DE), identified by MALDI-TOF mass spectrometer (MS) and validated by quantitative real-time polymerase chain reaction (qRT-PCR). (biomedcentral.com)
GTT treatment on HepG2 cells showed a total of five differentially expressed proteins when compared to their respective untreated cells where three proteins were down-regulated and two proteins were up-regulated. (biomedcentral.com)
Validation by qRT-PCR however showed decreased expression of Prx4 mRNA in HepG2 cells following GTT treatment. (biomedcentral.com)

More interestingly, the biosynthesized ZnO NPs displayed remarkable selective cytotoxicity against all tested cancer cell lines without any effect on normal cells. (springeropen.com)

In fact, several studies have shown that T2D induces senescence in multiple types of cells, including fibroblasts and endothelial cells ( 11 , 12 ). (frontiersin.org)
Microbial sensing by toll-like receptors (TLR) on liver sinusoidal endothelial cells (LSECs) results in a CXCL9 chemokine gradient that attracts Kupffer cells closer to the periportal region to ensure immune surveillance and host defense from invading pathogens arriving through the portal vein circulation. (encyclopedia.pub)
Hsieh SL, Wang JJ, Su KH, Kuo YL, Hsieh S, Wu CC*. Suppressive effects of Gynura bicolor ether extract on endothelial permeability and leukocyte transmigration in human endothelial cells induced by TNF-α. (nutr-metab-pu.org)

The high levels of NQO1 expression in many human solid tumors compared to normal tissue ensures their selective activation within tumor cells. (wikipedia.org)

To identify signaling factors between macrophages and pancreatic cancer cells (PCCs), a 97-target cytokine array and the TCGA-GTEx database were utilized. (bvsalud.org)

ER alerts a self-protective mechanism that is called ER stress during nutrient deprivation, pathogen infection, alterations in redox status, intraluminal Ca 2+ levels and folding defective protein conditions ( 9 ). (spandidos-publications.com)

Fluorescent staining assays revealed characteristic nuclear condensation, and marked decrease in mitochondrial outer membrane potential in treated cells. (afpm.org.my)

However, emerging evidence suggests that autophagy is a primary mechanism of cell death (autophagic cell death, ACD) and implicates ACD in several aspects of mammalian physiology, including tumor suppression and psychological disorders. (nature.com)
Autophagy occurs during normal development at the basal level, as well as under stress conditions. (nature.com)
Autophagy is generally considered as a cell survival/protection mechanism because it removes toxic or obsolete proteins and organelles and recycles the degradation products for use as sources for energy and metabolites in anabolic pathways 3 . (nature.com)
Therefore, the relationship between autophagy and cell death remains unclear and warrants further study to harness autophagy for the treatment of various human diseases. (nature.com)
Therefore, in this review, we avoid a lengthy repetition of the description of autophagy and cell death processes and focus on the death-promoting roles of autophagy and the intertwined connection between autophagy and apoptosis. (nature.com)
however, purvalanol triggered autophagy, which functions as a cell survival mechanism at early time-points. (spandidos-publications.com)

This inhibits the expression of ATP-binding cassette transporters, which are responsible for chemoresistance in cancer cells. (bvsalud.org)
The p53 protein is a transcription factor that normally inhibits cell growth and stimulates cell death in response to myriad stressors, including DNA damage (induced by either UV or chemical agents such as hydrogen peroxide), oxidative stress, and deregulated oncogene expression [6-10]. (bioinf.org)
Taken together LCH inhibits the growth of Ox-sensitive and Ox-resistant CRC cells by targeting EGFR and AKT, and inducing ROS generation and ER stress-mediated apoptosis. (biomolther.org)

Obtaining quantitative gene-expression of cells in their native context has significantly advanced through single-cell RNA sequencing technologies along with mechanical and enzymatic tissue manipulation. (molcells.org)

Here, we report an unexpected messenger role for heat shock protein 60 (HSP60) as a mitochondrial-releasing protein factor that couples stress-sensing signaling and cell survival machineries. (ewha.ac.kr)
Thus, this study reveals a novel mitonuclear survival axis responding to oxidative stress. (ewha.ac.kr)

The optimized formula was also able to significantly increase the percentage of cell population in both early and late stages of apoptosis, characterized by a higher intracellular caspase-3 concentration, as well as percentage of necrotic cells. (carusogiuseppe.net)
Further, pathway enrichment analysis revealed abundance and significantly modulated metabolites associated with oxidative stress pathways. (afpm.org.my)
Moreover, IR-SF significantly down-regulated Bcl-2, Bcl-w, survivin, xIAP and HSPs pro-proteins and induced DNA fragmentation and G0/G1-phase arrest in HCT 116 cells. (afpm.org.my)

These SP constitute an important ingredient of cell walls and get harvested by suitable extraction or precipitation method, followed by purification, characterization and biological studies (Fig. 2 ). (springer.com)

Conclusions These findings ascertained an conversation between p53, c-myc, p21, p27, Bcl-2, PI3K/Akt pathway, and CF-induced apoptosis in MSTO-211H and HCT-116 cells, suggesting that CF acts as an important regulator of cell growth in human malignancy cell lines. (bioinf.org)

p53 activation is characterized by a drastic increase and its rapid accumulation in stressed cells [11]. (bioinf.org)

Compounds isolated in freeze-dried açaí extract have been shown to enter human cells and neutralize free radicals at very low doses. (lifeextension.com)
Chandrasekhar, K., Kapoor, J. und Anishetty, S.: A prospective, randomized double-blind, placebo-controlled study of safety and efficacy of a high-concentration full-spectrum extract of ashwagandha root in reducing stress and anxiety in adults. (natur-wiki.de)
Lopresti, A. L. et al: An investigation into the stress-relieving and pharmacological actions of an ashwagandha (Withania somnifera) extract: A randomized, double-blind, placebo-controlled study. (natur-wiki.de)

Nevertheless, the definition of autophagic cell death (ACD) has been neither universally understood nor unanimously accepted in the field 4 . (nature.com)
Lacking proteosomal function on aggregates of unfolded proteins, ER stress may induce autophagic machinery. (spandidos-publications.com)

CDKs show their action by interacting with cyclins and different CDK-cyclin complexes regulate the cell cycle in the G1, S and G2/M phases ( 1 ). (spandidos-publications.com)
Furthermore, since they shown many structural features similar to fluorescent charge transfer complexes, we opted to check most of them in a fluorescence spectral scan of entire cells to determine whether these substances could be found in membrane transportation assays. (technumber.com)

In conclusion, the current study revealed the expression profiles of human TRP channels in 60 different breast cancer tissues and cell lines and furthermore validated the antitumor activity of TRPV1 against SUM149PT breast cancer cells, indicating that activation of TRPV1 could be used as a therapeutic target, even in the most aggressive breast cancer types. (dovepress.com)

Purvalanol is a purine-derived CDKI that binds with a high selectivity and competitively to the ATP binding side of CDK1/2 and leads to G2/M cell cycle arrest ( 4 ). (spandidos-publications.com)
Senescence is a stress-response cellular state characterised by proliferative arrest but active metabolism ( 7 ). (frontiersin.org)
2002). G(1) and G(2) cell-cycle arrest following microtubule depolymerization in human breast cancer cells. (sciendo.com)
Al- though quercetin induced cell death in a dose-dependent manner, 12.5-50 µ M quercetin inhibited the activity of both taxol and noco- dazole to induce G2/M arrest in various cell lines. (vdocuments.net)

The combination of gemcitabine and mithramycin shows potential as a therapeutic strategy for treating PAAD in cell lines, PDOs, and mouse models. (bvsalud.org)
This has especially been true for therapeutic agents that induce oxidative stress as the mechanism of action, as antioxidants may also re- duce the side effects of chemotherapeutic agents. (vdocuments.net)

Antimelanogenic, antioxidant and antiproliferative effects of antrodia camphorata fruiting bodies on B16-F0 melanoma cells. (nutr-metab-pu.org)

In contrast, the cancer cell lines were not affected by the ALE of C. citratus at any concentrations tested. (springeropen.com)

Age-related and metabolic diseases such as type 2 diabetes (T2D) represent a source of cellular stress due to their disruptive effect on normal physiological processes and, therefore, can induce premature senescence ( 10 ). (frontiersin.org)
The involvement of multiple signaling pathways (NF-κB and JNK) and proteins (EGF-R, Id-1 and Id-3) was suggested to explain the antiproliferative effect of GTT in prostate cancer (PCa) cells [ 14 ]. (biomedcentral.com)
The antiproliferative effect of GTT in rat hepatoma dRLh-84 cells and human hepatoma Hep3B cells were reported by Sakai et al. (biomedcentral.com)
We examined the effect of the cotreatment of cancer cells with the ﬂavonoid quercetin and 2 antimicrotubule drugs, namely, taxol and nocodazole. (vdocuments.net)
We hypothesized an additive or a synergistic effect with this drug-ﬂavonoid com- bination, but unexpectedly, quercetin protected cells from the activity of these antimicrotubule drugs and sustained the via- bility of the cells. (vdocuments.net)

Despite the short-term protection of cells by quercetin, colony formation and clonogenicity of HCT116 cells were still suppressed by quercetin or quercetin- taxol combination. (vdocuments.net)

HSC, hepatic stellate cell. (encyclopedia.pub)

Furthermore, single-cell sequencing data from the GEO database were analyzed to explore the crosstalk between PCCs and macrophages. (bvsalud.org)

LCH induced ROS generation and increased the expression of the ER stress markers. (biomolther.org)

Increased levels of polyamines in cells are considered to be involved in cancer progression. (spandidos-publications.com)
In conclusion, the new formulation of FBX into EMLs improved all the parameters related to the anti-proliferative activity and the toxic potential of the drug towards colorectal cancer cells. (carusogiuseppe.net)
Hence, the present study aimed to develop a cell membrane-camouflaged and BA-loaded polylactic-co-glycolic acid nanoparticle (CBAP) and assess its potential to counter chemoresistance in pancreatic cancer. (bvsalud.org)
Natural products have been shown to induce the expression of apoptosis regulators that are blocked in cancer cells. (afpm.org.my)
Suppression of carnosine on adhesion and extravasation of human colorectal cancer cells. (nutr-metab-pu.org)
Hsieh SL, Hsieh S, Kuo YH, Wang JJ, Wang JC, Wu CC*. Effects of Panax notoginseng on the metastasis of human colorectal cancer cells. (nutr-metab-pu.org)
Being able to specifically eliminate pro-cancer fibroblasts requires a better understanding of the factors that shape the role of these cells, and how to identify them. (elifesciences.org)
Thus, we identify bimodal activity of the ﬂavonoid quercetin, a short-term activity that is cytoprotective against chemotherapeutic drugs and a long-term activity that is inhibitory to cancer cell growth. (vdocuments.net)
The critical challenge in cancer treatment is how to effectively kill cancer cells without affecting healthy cells. (hindawi.com)
These effects of honey have been thoroughly investigated in certain cancers such as breast, liver and colorectal cancer cell lines. (mdpi.com)
cancer cell line: MCF7. (arjournals.org)
In this study, stimulation by the TRPV1 agonist, capsaicin, of SUM149PT cells, a model system for the most aggressive breast cancer subtype, triple-negative breast cancer, led to intracellular calcium signals that were diminished by the specific TRPV1 antagonist, capsazepin. (dovepress.com)
Transient receptor potential (TRP) channels, which are membranous ion channels that conduct calcium and sodium ions, have been shown to influence cancer cell growth. (dovepress.com)
The anticancer activity of biosynthesized ZnO NPs and C. citratus ALE was in vitro tested against seven human cancer cell lines (HCCL) (i.e. (springeropen.com)

All somatic cells proliferate via a mitotic process determined by progression through the cell cycle. (bioinf.org)
This antagonism of microtubule-targeting drugs was accompanied by a delay in cell cycle progression and inhi- bition of the buildup of cyclin-B1 at the microtubule organizing center of treated cells. (vdocuments.net)

Programmed cell death (PCD), as described by Lockshin and Williams 14 , is defined as controlled cell death evoked by intracellular systems. (nature.com)

The expression of E-cadherin and N-cadherin was increased and decreased, respectively, in both OSCC cell lines. (jcpjournal.org)
To overcome GR, inhibitors targeting the macrophage-CCL5-Sp1-AREG feedback loop were evaluated in cell lines, PDOs, and orthotopic mouse models of pancreatic carcinoma. (bvsalud.org)
The results from the PDO model were corroborated with cell lines, mouse models, and clinical data. (bvsalud.org)

Anatomy24

Organisms5

Diseases12

Chemicals and Drugs112

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes47

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Health Care3

Inflammation3

Apoptosis in HCT-116 cells1

Proliferation16

Physiological and pathological conditions2

Epithelial cells1

Rats5

Inhibition4

Squamous cell carc1

Colon5

Suppresses4

Viability4

Spatial4

Apoptotic7

HepG24

Cytotoxicity1

Endothelial cells3

Tumors1

Cytokine1

Redox1

Mitochondrial outer membrane1

Autophagy6

Inhibits3

Gene expression1

Survival2

Significantly3

Characterization1

Pathway1

Accumulation1

Extract3

Autophagic2

Complexes2

Tissues1

Arrest4

Therapeutic2

Melanoma cells1

Contrast1

Effect5

Colony1

Hepatic1

Macrophages1

Markers1

Cancer14

Progression2

Intracellular1

Lines3