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  • cancerous colon
  • Similar research that Boger utilized for comparison to his results involving elimination of cancerous tumors and antigens was centered around the use of similar immunoconjugates that were introduced to cancerous colon cells. (wikipedia.org)
  • tumor cells
  • F) H & E staining on sections from embedded lung and liver tissues showed that high amount of tumor cells infiltrated into lungs and livers obtained from VASH1 shRNA treatment group, but not from the control shRNA treatment group. (nih.gov)
  • These data indicated that silence of VASH1 expression in tumor cells directly enhanced tumor growth and tumorigenesis in vivo. (nih.gov)
  • The duocarmycin analogues are able to exert their mode of action at any phase in the cellular cycle, whereas tubulin binders will only attack tumor cells when they are in a mitotic state. (wikipedia.org)
  • Another important benefit is that, unlike other drug classes, duocarmycins can be effective against tumor cells that are multi-drug resistant. (wikipedia.org)
  • Using this technology scientists aims to create ADCs having an optimal therapeutic window, balancing the effect of potent cell-killing agents on tumor cells versus healthy cells. (wikipedia.org)
  • The high levels of NQO1 expression in many human solid tumors compared to normal tissue ensures their selective activation within tumor cells. (wikipedia.org)
  • inhibits
  • Radioactive 125 I seed inhibits the cell growth, migration, and invasion of nasopharyngeal carcinoma by triggering DNA damage and inactivating VEGF-A/ERK signaling," PLoS ONE , vol. 8, no. 9, Article ID e74038, 2013. (hindawi.com)
  • accumulation
  • HCT116 cells were treated with different doses of API (0.5, 1, 5, and 10 μM) for 48 h, harvested and stained with propidium iodide and subjected to flow cytometry.Histogram obtained indicated the accumulation cells in sub-G1 phase. (nih.gov)
  • protein
  • The ultimate proof that a cell line does not express the gene of interest is protein expression analysis by Western blot or similar. (horizondiscovery.com)
  • Unfortunately, due to the costs and time involved in optimising any new antibody , Horizon cannot test protein expression in all its cell lines. (horizondiscovery.com)
  • As such we recommend that protein expression is assessed in cell lines prior to proceeding with downstream experiments. (horizondiscovery.com)
  • The biosynthesis of BC200 RNA occurs at the cell body of a neuron and requires upstream promoter elements, downstream internal promoter elements (intragenic A and B boxes), at least two transcription factor binding sites, a TATA-like sequence, TATA-box binding protein (TBP), and RNA polymerase III. (wikipedia.org)
  • It has been shown previously that in cells treated with adozelesin, replication protein A (RPA) activity is deficient, and the middle subunit of RPA is hyperphosphorylated. (wikipedia.org)
  • Lymphokine-activated killer T-cell-originated protein kinase is an enzyme that in humans is encoded by the PBK gene. (wikipedia.org)
  • line
  • Cell lines are provided with a matched wild type control parental cell line ensuring phenotypes can be attributed directly to the genotype. (horizondiscovery.com)
  • Cell lines are provided as isogenic pairs - the modified mutant line and the originating wild type parental line. (horizondiscovery.com)
  • Do you provide the PCR conditions for your cell line genomic validation? (horizondiscovery.com)
  • The avrainvillamide and stephacidins family of structurally complex anticancer natural products are active against the human colon HCT-116 cell line. (wikipedia.org)
  • Although disease mutations can be harvested directly from cancer patients, these cells usually contain many background mutations in addition to the specific mutation of interest, and a matched normal cell line is typically not obtained. (wikipedia.org)
  • gene
  • Isogenic cell lines are created via a process called homologous gene-targeting. (wikipedia.org)
  • A recent key advance was discovered using AAV-homologous recombination vectors, which increases the low natural rates of HR in differentiated human cells when combined with gene-targeting vectors-sequences. (wikipedia.org)
  • Diagram of a typical rAAV vector (source: https://www.horizondiscovery.com/gene-editing/raav) Factors leading to the recent commercialization of isogenic human cancer cell disease models for the pharmaceutical industry and research laboratories are twofold. (wikipedia.org)
  • isogenic
  • They are provided with a genetically matched 'normal cell' to provide an isogenic system to research disease biology and novel therapeutic agents. (wikipedia.org)
  • autophagy
  • p53 Mediates colistin-induced autophagy and apoptosis in PC-12 cells," Antimicrobial Agents and Chemotherapy , vol. 60, no. 9, pp. 5294-5301, 2016. (hindawi.com)
  • N. M. Mazure and J. Pouysségur, "Hypoxia-induced autophagy: cell death or cell survival? (hindawi.com)
  • genetically
  • Horizon's X-MAN® (Mutant And Normal) cell lines are genetically engineered cell pairs containing defined genomic modifications, providing a model system for the study of the role of genes in cellular processes and drug responses. (horizondiscovery.com)
  • selective
  • A recent study has been published which describes the synthesis of water-soluble salts of dFBr and its action has been confirmed as selective potentiator of α4β2 nicotinic acetylcholine receptor responses by using two-electrode voltage clamp whole cell recordings. (wikipedia.org)
  • genomic
  • All X-MAN Cell Lines are validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. (horizondiscovery.com)
  • The primer sequences used for our internal genomic validation are provided on the Certificate of Analysis and Datasheet provided with the cell lines. (horizondiscovery.com)
  • arrests
  • Adozelesin is an alkylating minor groove DNA binder that is capable of rapidly inhibiting DNA replication in treated cells through a trans-acting mechanism and preferentially arrests cells in S phase. (wikipedia.org)
  • Rapidly
  • Rapidly thaw cells in a 37°C water bath. (horizondiscovery.com)
  • Stephacidin B rapidly converts into the electrophilic monomer avrainvillamide in cell culture, and there is evidence that the monomer avrainvillamide interacts with intracellular thiol-containing proteins, most likely by covalent modification. (wikipedia.org)
  • genes
  • WWC2 alias, BOMB, was researched in a grant suggesting that BOMB, along with two other genes (APOL6 and APOL1) promoted cell death in p53-null HCT116 cells. (wikipedia.org)
  • HR plays a major role in eukaryotic cell division, promoting genetic diversity through the exchange between corresponding segments of DNA to create new, and potentially beneficial combinations of genes. (wikipedia.org)
  • origin
  • Additionally, experiments have demonstrated important and significant differences in the ability to transform cells, compared with cells of murine origin. (wikipedia.org)
  • unknown
  • Vasohibin-1 (VASH1) is an endogenous angiogenesis inhibitor.However, the clinical relevance of VASH1 in colon cancer and its regulations on cancer angiogenesis and cancer cell biological characteristics are still unknown. (nih.gov)
  • mouse
  • mir-143 was found to be the most enriched miRNA in mouse embryonic stem cells that were differentiating into cardiac progenitor cells. (wikipedia.org)
  • role
  • This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. (wikipedia.org)
  • drug
  • This makes them suitable for maximizing the cell-killing potency of antibody-drug conjugates to which they are attached. (wikipedia.org)
  • found
  • In humans, it is found in neuropil areas which are composed of predominantly unmyelinated dendrites, axons, and glial cells. (wikipedia.org)
  • In the periphery, neurotensin is found in enteroendocrine cells of the small intestine, where it leads to secretion and smooth muscle contraction. (wikipedia.org)
  • agents
  • Growing evidence suggests that DNA damaging agents, such as duocarmycins, are more efficacious in tumor cell killing than tubulin binders, particularly in case of solid tumors. (wikipedia.org)
  • biosynthesis
  • The biosynthesis of BC200 RNA occurs at the cell body of a neuron and requires upstream promoter elements, downstream internal promoter elements (intragenic A and B boxes), at least two transcription factor binding sites, a TATA-like sequence, TATA-box binding protein (TBP), and RNA polymerase III. (wikipedia.org)
  • vitro
  • And wound healing and transwell assay were applied to detect the metastasis potential of C26 and HCT116 colon cancer cell lines in vitro. (readbyqxmd.com)
  • Using the oxygen-glucose deprivation (OGD) model of hippocampal neuron ischemia in vitro, we found that the overexpression of miR-134 mediated by recombinant adeno-associated virus (AAV) vector infection significantly promoted neuron death induced by OGD/reoxygenation, whereas the inhibition of miR-134 provided protective effects against OGD/reoxygenation-induced cell death. (jove.com)
  • colon cancer cell lines
  • Additionally, in a novel strategy with biological approach for synthesis of silver nanoparticles (AgNPs), a toxic substance, using sodium para-hydroxybenzoate tetrahydrate (SPHT) isolated from Vitex negundo leaves, treatment of sodium para-hydroxybenzoate tetrahydrate (SPHT) improved the hydroxyl and carboxylic functional groups mediators in edhibited a stronger ability towards synthesis of AgNPs which indicated the apoptotic effect on human colon cancer cell lines. (blogspot.com)
  • L leaves and its apoptotic effect on human colon cancer cell lines by Durai P1, Chinnasamy A2, Gajendran B1, Ramar M1, Pappu S3, Kasivelu G4, Thirunavukkarasu A5. (blogspot.com)
  • tumor cell
  • The irreversible alkylation of DNA disrupts the nucleic acid architecture, which eventually leads to tumor cell death. (wikipedia.org)
  • Growing evidence suggests that DNA damaging agents, such as duocarmycins, are more efficacious in tumor cell killing than tubulin binders, particularly in case of solid tumors. (wikipedia.org)
  • apoptotic
  • In contrast, both low and high bizelesin concentrations enhanced p53 and p21 induction and triggered G(2)-M cell cycle arrest and eventual senescence without significant apoptotic cell death. (wikipedia.org)
  • Paraptosis is a form of type III programmed cell death with a unique combination of certain apoptotic and necrotic characteristics. (wikipedia.org)
  • receptor
  • Pubmed ID: 17322387 To study the effect of enhanced glucocorticoid signaling on T cells, we generated transgenic rats overexpressing a mutant glucocorticoid receptor with increased ligand affinity in the thymus. (jove.com)
  • DcR3 is a soluble receptor secreted by cells and binds with Fas ligand ( FasL) . (pathwaymaps.com)
  • The mobile viability facts from Figure 4 exhibits that the transportation of ¯unisolide is not brought on by poisonous e.ects of the compounds on the Calu Estrogen Receptor Pathway three cells, indicating that the observed transport is not due to a reduced integrity of the monolayers. (mtor-inhibitors.com)
  • Shorten ciliary resorption w W For the duration of the development from G2 to M period Nnte k for the instant, the mobile cycle is critical because accessibility Modify method is development factor receptor cells with cilia. (mtor-inhibitors.com)
  • Dissociated HL60 cells, neutrophils as T-cell line is the polarity T of each divergent and contradictory tze S of the two signals, the loan of a solitary variety of receptor-mediated st. (mtor-inhibitors.com)
  • A recent study has been published which describes the synthesis of water-soluble salts of dFBr and its action has been confirmed as selective potentiator of α4β2 nicotinic acetylcholine receptor responses by using two-electrode voltage clamp whole cell recordings. (wikipedia.org)
  • human colon
  • These range from the hepatoxic brevianamide F (cyclo(L-Trp-L-Pro)) to the annulated tremorogenic verruculogen and the spiro-annulated spirotryprostatin B which represent a promising class of antimitotic arrest agents, to the structurally complex (+)-stephacidin A, a bridged 2,5-diketopiperazine that possess a unique bicyclo[2.2.iazaoctane core ring system and is active against the human colon HCT-116 cell line. (wikipedia.org)
  • The avrainvillamide and stephacidins family of structurally complex anticancer natural products are active against the human colon HCT-116 cell line. (wikipedia.org)
  • monolayers
  • On the other hand, the speci®c Pgp inhibitors verapamil, SDZ PSC 833 and LY335979 showed ratios of precisely 1 unit suggesting that Pgp is included in the transport of a unisolide across Calu 3 cell monolayers. (mtor-inhibitors.com)
  • Antibody
  • Immunocytochemistry of BUB3 in Jurkat cells with BUB3 polyclonal antibody ( Cat # PAB12997 ) at 10 µg/ml. (acris-antibodies.com)
  • Western blot analysis of BUB3 in Jurkat cell lysate with BUB3 polyclonal antibody ( Cat # PAB12997 ) at ( A ) 0.5 and ( B ) 1 µg/ml. (acris-antibodies.com)
  • Western blot analysis of extracts from HeLa cells/Jurkat cells/293 cells, using BUB3 Antibody. (acris-antibodies.com)
  • The cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). (acris-antibodies.com)
  • Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. (acris-antibodies.com)
  • Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. (acris-antibodies.com)
  • This makes them suitable for maximizing the cell-killing potency of antibody-drug conjugates to which they are attached. (wikipedia.org)
  • differentiation
  • A look at the mobile cycle may be asking yourself no matter whether to use the choice of the require to restore the database as MTOC in mitosis base or to neutralize a MTOC in submit-mitotic cells, so it differentiation condition matter the foundation. (mtor-inhibitors.com)
  • The procedure of differentiation of the K Body and can lead centrosome sequential displacement of the elements to the mobile cycle stage dd Embroidered most teams of proteins result in k Ter Sp can slow phase, the cell cycle can be changed. (mtor-inhibitors.com)
  • viability
  • citation needed] Cucurbitacin possesses dose-dependent anti-inflammatory activity related to its inhibition of nitric oxide (an RNS) production in macrophages without affecting the viability of these cells. (wikipedia.org)
  • Collectively
  • Collectively, our findings suggest that endogenous glucocorticoids impact T-cell development and favor the selection of Th2- over Th1-dominated adaptive immune responses. (jove.com)
  • MAPK
  • Following exposure of SKGT-4 cells to DCA, protein levels of COX-2, MAPK and PARP were examined by immunoblotting. (beds.ac.uk)
  • reactive
  • This reaction ensures complete oxidation of the substrate without the formation of semiquinones and species with reactive oxygen radicals that are deleterious to cells. (wikipedia.org)
  • Reduction
  • Reduction of stability in the tumor cells might suggest mobile cycle. (mtor-inhibitors.com)
  • Selenols are often generated by reduction of diselenides followed by protonation of the resulting selenoate: 2 RSeSeR + 2 LiHB(C2H5)3 → 2 RSeLi + 2 B(C2H5)3 + H2 RSeLi + HCl → RSeH + LiCl Dimethyl diselenide can be easily reduced to methaneselenol within cells. (wikipedia.org)
  • It causes a reduction of cell multiplication. (wikipedia.org)
  • mitotic
  • The duocarmycin analogues are able to exert their mode of action at any phase in the cellular cycle, whereas tubulin binders will only attack tumor cells when they are in a mitotic state. (wikipedia.org)
  • therapeutic
  • Using this technology scientists aims to create ADCs having an optimal therapeutic window, balancing the effect of potent cell-killing agents on tumor cells versus healthy cells. (wikipedia.org)
  • citation needed] As cucurbitacin E doesn't affect normal human liver cells, it may have therapeutic potential and effective treatment for a variety of inflammation mediated diseases. (wikipedia.org)
  • cycle
  • In addition, not only to act as MTOCs centrosomes, but also a excellent e ep signaling for cell cycle regulators. (mtor-inhibitors.com)
  • In this context it is intriguing that most of the tumor cells of normal small molecule ALK inhibitor hair, no eyelashes Shore Zellvorl in every period of the cell cycle. (mtor-inhibitors.com)
  • Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. (wikipedia.org)
  • mutant
  • In the NCI-H2122 KRASG12C mutant non-small cell lung carcinoma (NSCLC) xenograft model, treatment with up to 5 mg/kg Cobimetinib (GDC-0973) lead to moderate TGI and at 10 mg/kg approaches tumor stasis . (medchemexpress.com)