Both encode large glycosylated proteins consisting of around 3400 amino acids and a molecular weight of around 390,000 Daltons, excluding the glycosylation. (wikipedia.org)
Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production. (wikipedia.org)
The devices and kits include one or more associators specific for molecular targets, such as microRNAs and proteins and peptide fragments thereof. (justia.com)
Antibody production4
Consequently, BSA is a popular carrier protein for conjugation to haptens and other weak antigens to make them more immunogenic for antibody production. (aatbio.com)
This ReadiLink™ BSA Conjugation kit is primarily optimized for the simple preparation of hapten-carrier conjugates for immunization and antibody production. (aatbio.com)
The resulting conjugate is used for eliciting an immune response and antibody production against the hapten. (aatbio.com)
Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production. (wikipedia.org)
Conjugate4
Purify the conjugate by desalting to remove non-reacted crosslinker and protein preservative (e.g., sodium azide). (aatbio.com)
If the Hapten-BSA conjugate is to be stored for more than a few days, sterile filter the conjugate, and store at 4 °C or -20 °C. (aatbio.com)
Aggregates remain immunogenic, but limit the ability to conjugate haptens, and are difficult to manipulate in the laboratory. (wikipedia.org)
Peptides2
It contains numerous sites per molecule for effective conjugation of peptides and other antigens using amine-reactive or carboxyl-reactive crosslinkers. (aatbio.com)
The carboxyl-reactive carbodiimide reacts with exposed carboxyl and amino groups on peptides and proteins to form stable bonds. (aatbio.com)
Carbodiimide3
The ReadiLink™ BSA Conjugation kit is one-step conjugation of a hapten to a carrier protein using the carboxyl-reactive carbodiimide as the crosslinker. (aatbio.com)
These kits contain BSA formulated in buffers compatible with the carboxyl-reactive carbodiimide reactions and desalt spin columns, which offer exceptional protein recovery by simple centrifugation step. (aatbio.com)
A simple one-step coupling can be performed using the carbodiimide crosslinker EDC to covalently attach carboxyls to primary amines. (wikipedia.org)
Amino1
Both encode large glycosylated proteins consisting of around 3400 amino acids and a molecular weight of around 390,000 Daltons, excluding the glycosylation. (wikipedia.org)
Immunogenic2
In addition, the 67 kDa protein is sufficiently large and complex to be fully immunogenic. (aatbio.com)
The KLH protein is potently immunogenic, but does not cause an adverse immune response in humans. (wikipedia.org)
Immune response1
Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens allows a high hapten:carrier protein ratio, increasing the likelihood of generating hapten-specific antibodies. (wikipedia.org)
Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of antibodies for research, biotechnology and therapeutic applications. (wikipedia.org)
Form1
The protein oligomerises to form a barrel shaped didecameric complex which is composed of 20 monomers. (wikipedia.org)
Solution4
Add the 450 µL hapten solution into the 200 µL BSA solution to have Hapten-BSA mixture solution. (aatbio.com)
Add the Hapten-BSA solution into one vial of EDC (10mg) (Component C), dissolve it by gentle mixing. (aatbio.com)
If the protein becomes denatured or if the copper ions are lost in the purification process, the opalescent blue color disappears and the solution becomes a dull grayish color. (wikipedia.org)
Denaturation of KLH also results in a tendency of the protein to aggregate and precipitate from solution. (wikipedia.org)
Addition1
In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically-based research techniques in mammalian model organisms. (wikipedia.org)
Size1
Optimize the protocol accordingly for the conjugation efficiencies upon the size and structure of your hapten. (aatbio.com)
Effective1
KLH is an effective carrier protein for several reasons. (wikipedia.org)