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Ubiquitin-Activating EnzymesAcyl Carrier ProteinUbiquitinsHaptensCarrier ProteinsPeptidesFatty Acid Synthase, Type II3-Oxoacyl-(Acyl-Carrier-Protein) SynthasePantetheineConjugation, GeneticAcyl-Carrier Protein S-MalonyltransferaseAmino Acid SequenceMolecular Sequence DataEnoyl-(Acyl-Carrier-Protein) Reductase (NADH)Escherichia coliCarrier State3-Oxoacyl-(Acyl-Carrier-Protein) ReductaseAcyltransferasesTransferases (Other Substituted Phosphate Groups)Drug CarriersTriclosanPolyketide SynthasesPeptide FragmentsCarbon-Sulfur LigasesBacterial ProteinsKineticsFatty Acid SynthasesFatty AcidsReduced Folate Carrier ProteinBase SequenceCloning, MolecularModels, MolecularPeptide LibraryLigasesSequence Homology, Amino AcidThiolester HydrolasesCoenzyme ASubstrate SpecificityProtein BindingPeptide SynthasesMembrane Transport ProteinsProtein ConformationMutationEnoyl-(Acyl-Carrier Protein) Reductase (NADPH, B-Specific)Binding SitesAcylationHeterozygote DetectionMitochondrial ADP, ATP TranslocasesRecombinant ProteinsAntimicrobial Cationic PeptidesCarbon-Nitrogen LigasesAcetyl-CoA C-AcetyltransferaseOligopeptidesProtein Structure, TertiaryHemocyaninBiotinCeruleninPeptides, CyclicHeterozygotePantothenic AcidEscherichia coli ProteinsBiological TransportMalonyl Coenzyme AChromatography, High Pressure LiquidPeptide MappingCrystallography, X-RayEpitopesGalactosidesAntibodiesPlasmidsAcetyl-CoA CarboxylaseMolecular WeightStructure-Activity RelationshipElectrophoresis, Polyacrylamide GelStreptomycesMalonatesProtein Structure, SecondaryVaccines, ConjugateMolecular StructureMass SpectrometrySequence AlignmentDinitrophenolsSerum Albumin, BovineDinitrobenzenesRabbitsMultienzyme ComplexesApoproteinsHydrogen-Ion ConcentrationRecombinant Fusion ProteinsCatalysisAntibody FormationCell MembraneAntibody SpecificityPlant ProteinsGenes, BacterialUbiquitin-Conjugating EnzymesLiposomesMice, Inbred BALB CGenetic Complementation TestMagnetic Resonance Spectroscopy
AntibodiesAntigensRecombinant proteinsConjugatesBioconjugationAntibody ServicesAminoConjugateFusion proteinsSerum albumin proteinBufferImmunizationSynthesisImmunogensPurificationMolecular weightOvalbuminSolubilityImmunogenicBacterialImmune responseSequenceTherapeuticSolubleSeparationProductionChemicalsIncludeKitsPrimaryConcentrationDrugVarietyDevelopmentStudiesCellsSolutionChickenShownFunction
Antibodies5
Antigens6
Recombinant proteins3
Conjugates3
  • This ReadiLink™ BSA Conjugation kit is primarily optimized for the simple preparation of hapten-carrier conjugates for immunization and antibody production. (aatbio.com)
  • This would include all purified proteins (natural or recombinant) or peptide-protein conjugates supplied to us or proteins as precipitates or in Sepharose-beads/bound forms. (atzlabs.com)
  • For the separation of proteins a variety of conjugates can be used with a high specificity for a certain matrix. (antibodies-online.com)
Bioconjugation1
  • CellMosaic provides personalized hapten-carrier bioconjugation kits for antibody production and immunoassay tools. (cellmosaic.com)
Antibody Services2
  • ADI is truly a global company providing custom peptide and antibody services throughout the US and many international institutions. (atzlabs.com)
  • Please consult Some Commonly Asked Questions About Custom Peptide And Antibody Services for bleed volume in other species and optional services. (atzlabs.com)
Amino2
  • The carboxyl-reactive carbodiimide reacts with exposed carboxyl and amino groups on peptides and proteins to form stable bonds. (aatbio.com)
  • CRM 197 , which has a single amino acid substitution of glutamic acid for glycine, is a well-characterized carrier protein and is utilized in a number of approved conjugate vaccines for diseases such as meningitis and pneumococcal bacterial infection. (genengnews.com)
Conjugate6
  • The resulting conjugate is used for eliciting an immune response and antibody production against the hapten. (aatbio.com)
  • Purify the conjugate by desalting to remove non-reacted crosslinker and protein preservative (e.g., sodium azide). (aatbio.com)
  • The Hapten-BSA conjugate can now be used for immunization. (aatbio.com)
  • If the Hapten-BSA conjugate is to be stored for more than a few days, sterile filter the conjugate, and store at 4 °C or -20 °C. (aatbio.com)
  • Access to clinically-proven, safe, and efficacious carrier proteins is critical for research in the field of conjugate vaccines. (genengnews.com)
  • Conjugate Epitope density, measured by acid hydrolysis and RP-HPLC, showed that the conjugation achieved with recombinant CRM 197 is indeed comparable to that obtained from the native organism. (genengnews.com)
Fusion proteins1
Serum albumin protein2
Buffer1
  • Dissolve up to 2 mg hapten in 450 µL Conjugation Buffer (Component B). (aatbio.com)
Immunization1
Synthesis1
  • The crystal structure of diphtheria toxin (DT) reveals that the molecule consists of three domains: a catalytic domain (fragment A), a transmembrane domain, and a receptor-binding domain (both in fragment B). Mild trypsinization and reduction of the native molecule in vitro results in two fragments, A and B. Fragment A is a NAD+ binding enzyme that inhibits protein synthesis. (genengnews.com)
Immunogens1
  • One of the key molecular events in skin sensitization is protein haptenation, i.e. the chemical modification of self-skin protein(s) thus forming macromolecular immunogens. (cdc.gov)
Purification1
  • Affinity Purification: ADI can couple the provided antigen (peptide, proteins) to prepare the affinity matrix. (atzlabs.com)
Molecular weight1
Ovalbumin3
Solubility1
  • For example, ficoll has been intensively studied as a carrier for CpG adjuvants, which has been shown in adjuvants to include size (a key determinant of delivery to the phagocytic system of macrophages), high water solubility, capacity for a variety of surface chemistries, and the expected advantages such as immunogenicity and toxicity and ease of lyophilization. (cd-bioparticles.net)
Immunogenic1
  • In addition, the 67 kDa protein is sufficiently large and complex to be fully immunogenic. (aatbio.com)
Bacterial1
  • The antimicrobial wasp Polybia-MP1 peptide selectively kills bacterial cells! (biosyn.com)
Immune response1
  • Carrier proteins both increase the magnitude of the immune response as well as engender B-cell "memory. (genengnews.com)
Sequence2
  • Peptide mapping at 97.4% sequence coverage shows high similarity between the two proteins. (genengnews.com)
  • [ 87 ] The same team later found that the inclusion of an EGF peptide sequence could facilitate transport to the brain tumors. (medscape.com)
Therapeutic2
  • This work supported both small-molecule high-throughput screening and protein therapeutic efforts. (chi-peptalk.com)
  • Subsequently, he worked as a postdoctoral researcher in the group of Prof. T.P.J. Knowles at the Department of Chemistry at the University of Cambridge, U.K. His research interests include the development, characterization and formulation of therapeutic proteins. (chi-peptalk.com)
Soluble1
Separation1
  • In combination with a protein or peptide they are used for separation but also for the detection of the protein used or to distinguish between native and enhanced expression. (antibodies-online.com)
Production2
  • Download Documents SDS USER MANUAL COA_Activated KLH Lots11.0126 Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein for antibody production. (cellmosaic.com)
  • Rich Altman has 30 years of experience in protein expression and production. (chi-peptalk.com)
Chemicals2
  • In affected indivi- chemicals possessing the ability to react with duals, it has a serious impact on their quality of proteins, and hence these methods do not esti- life. (cdc.gov)
  • The basis of hapten- molecular mechanisms of the sensitization pro- protein binding work is the hypothesis that upon cess will result in novel opportunities for the skin absorption, only protein-reactive chemicals development of alternative methods for assessing (or those that can be metabolically or chemically skin sensitization hazard and relative potency of converted to protein-reactive species) are able to chemicals. (cdc.gov)
Include1
Kits1
  • These kits contain BSA formulated in buffers compatible with the carboxyl-reactive carbodiimide reactions and desalt spin columns, which offer exceptional protein recovery by simple centrifugation step. (aatbio.com)
Primary1
  • It was later found that its conjugation to a hapten can generate a strong antigen-specific primary immune memory. (cd-bioparticles.net)
Concentration1
  • Higher concentration of DMSO might irreversibly denature the carrier protein. (aatbio.com)
Drug2
  • Illustrations of three main noncovalent drug-loading schemes including (A) hydrophobic, (B) electrostatic and (C) physical confinement-based drug conjugation. (medscape.com)
  • He obtained his PhD in Biochemistry from Northwestern University in 2008 where he studied protein structure-based drug design of novel chemotherapeutic agents. (chi-peptalk.com)
Variety1
  • The following protocol is a general protocol for a wide variety of haptens. (aatbio.com)
Development1
  • In this review, we consider some of the theoretical aspects of protein haptenation, how mechanisms of protein haptenation can be investigated experimentally and how we can use such knowledge in the development of novel, alternative approaches for predicting skin sensitization potential in the future. (cdc.gov)
Studies2
  • A series of comparability studies were performed, comparing both the structure and function of P. fluorescens recombinant CRM 197 to the protein produced in the native organism, C. diphtheriae . (genengnews.com)
  • They named these proteins "fodrin" as antibody studies showed that they were localized in the sheath under the axonal membrane, but not in the core of the axon. (biocheminfo.org)
Cells2
  • The role of the carrier protein is to enhance immunogenicity by providing T-cell epitopes via MHC Class II presentation to T-helper cells. (genengnews.com)
  • The spectrin family of proteins were originally discovered as major components of the submembraneous cytoskeleton of osmotically lyzed red blood cells. (biocheminfo.org)
Solution3
  • 30% in the final conjugation solution) to dissolve it first. (aatbio.com)
  • Add the 450 µL hapten solution into the 200 µL BSA solution to have Hapten-BSA mixture solution. (aatbio.com)
  • Add the Hapten-BSA solution into one vial of EDC (10mg) (Component C), dissolve it by gentle mixing. (aatbio.com)
Chicken2
  • Chicken egg albumin is the major protein constituent of egg whites and has a MW of. (cellmosaic.com)
  • Chicken egg albumin is the major protein constituent of egg whites and has a MW of 42.7 KDa. (cellmosaic.com)
Shown1
  • have shown that PEGylated polyacrylamide hydrogel particles could be loaded with Coomassie Blue and provide specific tumor cell staining when targeting peptides were incorporated. (medscape.com)
Function1
  • Epitope tags are usually engineered onto either the N- or C- terminus in order to minimize tertiary structure disruptions that may alter protein function. (antibodies-online.com)