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  • double haploid
  • Additionally a double haploid plant was obtained spontaneously from this haploid line. (nih.gov)
  • Twenty-eight Double Haploid (DH) lines were mechanically inoculated with CMV and the level of resistance was evaluated by using DAS-ELISA. (thescipub.com)
  • Borràs-Gelonch G, Slafera GA, Casas AM, van Eeuwijkd F, Romagosa I. Genetic control of pre-heading phases and other traits related to development in a double-haploid barley (Hordeum vulgare L.) population. (csic.es)
  • We studied the genetic control of these two pre-heading sub-phases (from sowing to the onset of stem elongation, LS, and from then to heading, SE) in terms of Quantitative Trait Loci (QTL) in a barley double-haploid population derived from the cross Henni × Meltan, both two-rowed spring North European barley cultivars. (csic.es)
  • Genome
  • Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence. (nih.gov)
  • The use of homozygous lines presents a significant advantage for the accomplishment of sequencing projects.The first haploid plant of 'Clemenules' clementine produced directly by germination of a haploid embryo, which grew vigorously and produced flowers, has been obtained in this work.This haploid line has been selected and it is being used by the ICGC to establish the reference sequence of the nuclear genome of citrus. (nih.gov)
  • Due to the existence of important molecular resources and previous success in obtaining haploid clementine plants, haploid clementine was selected as the target for the implementation of the reference whole genome citrus sequence. (nih.gov)
  • This haploid line has been selected and it is being used by the ICGC to establish the reference sequence of the nuclear genome of citrus. (nih.gov)
  • Two important aspects of the in vivo DH system used in maize are as follows: (i) the identification of haploid progeny and (ii) doubling of the haploid genome to produce fertile inbred lines. (dtu.dk)
  • Haploids are a valuable tool for genomic studies in higher plants, especially those with huge genome size and long juvenile periods, such as conifers. (mdpi.com)
  • diploids
  • Researchers measured times from cytokinesis to budding (G1) and from budding to cytokinesis in haploids, diploids or tetraploids (mothers and daughters), using time-lapse fluorescence microscopy of strains expressing Myo1 tagged with green fluorescent protein (Myo1-GFP). (harvard.edu)
  • We tested for differentiation in fecundity and spore survival among the gametophyte males and females (haploids) and the tetrasporophytes (diploids). (springer.com)
  • The probability of a frond becoming fecund was size-dependent, highest for the haploid males and lowest for the haploid females, with the diploids displaying intermediate probabilities. (springer.com)
  • Fecund diploids released more tetraspores than carpospores released by the haploid females. (springer.com)
  • Gerstein AC, Cleathero LA, Mandegar MA, Otto SP (2010) Haploids adapt faster than diploids across a range of environments. (datadryad.org)
  • We measured the competitive fitness of all ancestral and evolved lines against a common competitor and find that in all seven environments haploid lines adapted faster than diploids, significantly so in three environments. (datadryad.org)
  • These results are consistent with theory that predicts haploids should evolve faster than diploids at large population sizes. (datadryad.org)
  • Maize
  • The use of doubled haploids (DHs) in maize has become ubiquitous in maize breeding programmes as it allows breeders to go from cross to evaluation in as little as 2Â years. (dtu.dk)
  • Currently, identification of maize haploid progeny is performed manually using the R1-nj seed colour marker. (dtu.dk)
  • Amrani N, Sarrafi A, Alibert G (1993) Genetic variability for haploid production in crosses between tetraploid and hexaploid wheats with maize. (springer.com)
  • Ayed S, Slama-Ayed O, da SJA T, Slim-Amara H (2011) Effect of different factors on haploid production through embryo rescue in durum wheat x maize crosses. (springer.com)
  • Bains NS, Singh J, Ravi, Gosal SS (1995) Production of wheat haploids through embryo rescue from wheat x maize crosses. (springer.com)
  • Campbell AW, Griffin WB, Burritt DJ, Conner AJ (2000) The effects of temperature and \ light intensity on embryo numbers in wheat doubled haploid production through wheat x maize rosses. (springer.com)
  • mutations
  • ASRM President Elect Christos Coutifaris, MD, PhD, commented, "This well-designed proof of concept study demonstrates the feasibility of correcting harmful mutations in haploid cells and has implications for correcting gametes. (reproductivefacts.org)
  • We obtained rough estimates of the average selection coefficients in haploids between 2-10% for these first selected mutations. (datadryad.org)
  • embryo
  • One of the haploids, obtained directly from an original haploid embryo, grew vigorously and produced flowers after four years. (nih.gov)
  • The first haploid plant of 'Clemenules' clementine produced directly by germination of a haploid embryo, which grew vigorously and produced flowers, has been obtained in this work. (nih.gov)
  • vitro
  • This model facilitated the derivation of haploid neural progenitor cells (haNPCs), which maintained haploidy and differentiation potential into neurons and glia for a long period in vitro . (biologists.org)
  • molecular
  • Here, we present an overview of the work done in conifers, with special emphasis on the production of a haploid cell line in maritime pine ( Pinus pinaster L.) and the use of a set of molecular markers, which includes Single Nucleotide Polymorphisms (SNPs) and microsatellites or Single Sequence Repeats (SSRs), to validate chromosomal integrity confirming the presence of all chromosomic arms. (mdpi.com)
  • crosses
  • By quantifying within-male variation in sperm length using crosses between males and females from populations that had been subjected to divergent experimental selection, we demonstrate that haploid gene expression does not contribute to variation in sperm length in both Drosophila melanogaster and Scathophaga stercoraria . (pubmedcentralcanada.ca)
  • stable
  • These strains are stable haploid derivatives and ura3 auxotrophs tagged with a 6-bp barcode, recognized by a restriction enzyme to allow easy identification. (nih.gov)
  • agricultural
  • Even with the comparatively modest budgets of those active in forage crop improvement, haploid and DH techniques can be developed into powerful tools to achieve the acceleration of the speed of genetic gain needed to meet future agricultural demands. (mdpi.com)
  • Research
  • The importance of haploid and doubled haploid (DH) techniques for basic and applied research, as well as to improve the speed of genetic gain when applied in breeding programs, cannot be overstated. (mdpi.com)
  • selection
  • Even with post-meiotic gene expression, it is altogether unclear how much of the phenotypic variation among sperm within an ejaculate is attributable to allelic variation among haplotypes, and hence amenable to haploid selection ( Joseph & Kirkpatrick 2004 ). (pubmedcentralcanada.ca)