Hanks' Balanced Salts Solutions (HBSS) are designed for short term use under ambient atmospheric conditions - not for CO 2 incubation. (jainbiologicals.com)
Hank's1
The non-inactivated virus transport media currently studied by Desheng is based on Hank's solution, and is also added with BSA (bovine serum albumin) amino acids, vitamins and other viruses needed nutrient content. (whdsbio.cn)
Invitrogen2
After washing, alkaline phos- in Hanks balanced salt solution medium (Invitrogen, Pais- phatase-conjugated goat anti-mouse IgG (Jackson Immuno ley, Scotland). (cdc.gov)
Liberase (0.3 mg/ml Roche Applied Science) dissolved in Hanks' buffered salt solution (Invitrogen) was injected into the pancreas through the bile duct. (cell-metabolism.com)
NaCl3
The exceptionally low corrosion rate (∼0.1 mm y-1 in concentrated NaCl solution for 7 days) enables lean Mg-Ca alloys great potential for diverse applications, particularly if relevant properties (e.g. mechanical strength, electrochemical performance, etc.) can be enhanced by thermomechanical processing. (researchgate.net)
Normal saline (NSS, NS or N/S) is the commonly used phrase for a solution of 0.90% w/v of NaCl, 308 mOsm/L or 9.0 g per liter. (wikipedia.org)
The solution is 9 grams of sodium chloride (NaCl) dissolved in water, to a total volume of 1000 ml (weight per unit volume). (wikipedia.org)
Saline solution2
Saline (also known as saline solution) is a mixture of sodium chloride (salt) and water. (wikipedia.org)
However, if the osmotic coefficient (a correction for non-ideal solutions) is taken into account, then the saline solution is much closer to isotonic. (wikipedia.org)
Cold storage in RPMI 1640 medium, a recommended cell culture medium for Vero-B4 cells, surprisingly, strongly enhanced cold-induced cell injury in these cells in comparison to cold storage in Krebs-Henseleit buffer or other cell culture media (DMEM, L-15 and M199). (biomedcentral.com)
Phosphate3
However, more extreme conditions (Ca 2+ was nominally absent and phosphate concentration raised to 25 mM as in the organ preservation solution University of Wisconsin solution) also increased cold-induced injury in rat hepatocytes and porcine aortic endothelial cells. (biomedcentral.com)
50% skim milk in 0.01 M phosphate-buffered saline. (hl7.org)
Sodium3
Because infusing a solution of low osmolality can cause problems such as hemolysis, intravenous solutions with reduced saline concentrations (less than 0.9%) typically have dextrose (glucose) added to maintain a safe osmolality while providing less sodium chloride. (wikipedia.org)
A variety of ReadiUse™ Probenecid products , Including water-soluble, sodium salt, and stabilized solutions, can be purchased from AAT Bioquest. (aatbio.com)
A 3.2% solution of Sodium Citrate in water. (hl7.org)
Physiological3
Less commonly, this solution is referred to as physiological saline or isotonic saline (because it is approximately isotonic to blood serum, which makes it a physiologically normal solution). (wikipedia.org)
Refer to sample experimental protocol for appropriate experimental/physiological buffers. (aatbio.com)
Chemically defined, Balanced Salt Solutions provide an environment that will maintain the structural and physiological integrity of mammalian cells in vitro. (jainbiologicals.com)
Incubate1
Then incubate the cells for 1 hour in the HEPES buffer saline. (dojindo.com)
DMSO3
3. Dilute 1 mM Fura 2-AM DMSO solution with HEPES buffer saline to prepare 1 mM Fura 2-AM working solution. (dojindo.com)
Prepare a 2 to 5 mM stock solution of Calcein UltraGreen™ AM in high-quality, anhydrous DMSO. (aatbio.com)
When reconstituted in DMSO, Calcein UltraGreen™ AM is a clear, colorless solution. (aatbio.com)
Detergent2
3. The method of claim 1, wherein said step of harvesting the amebocytes is performed by exposing the gill flap tissues to a solution selected from the group consisting of between approximately 10 and 20% Limulus serum, a detergent solution, copper sulfate solutions and combinations thereof. (gc.ca)
Inactivir® PLUS is a guanidine-based buffer, augmented by REACH-compliant detergent. (lifescienceproduction.co.uk)
Preparation1
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. (aatbio.com)
The solution exerts a softening and loosening influence on the mucus to make it easier to wash out and clear the nasal passages for both babies and adults. (wikipedia.org)
Remove the cell culture medium and wash cells once with serum-free buffer to remove any remaining media. (aatbio.com)
We compared various cell culture media/solutions for cold storage of Vero-B4 kidney cells, a cell line widely used in biotechnology. (biomedcentral.com)
Manufacturer, batch, medium supplements and the most likely components with concentrations outside the range of the other media/solutions (vitamin B 12 , inositol, biotin, p-aminobenzoic acid) did not cause this aggravation of cold-induced injury in RPMI 1640. (biomedcentral.com)
From buffer solutions to cell culture media formulations at bench top or production sales, Caisson Labs gives you the products you need in a way that makes sense to you. (caissonlabs.com)
For Xiaobai who is new to the knowledge of virus transport media , it is not so easy to distinguish the difference between virus transport media and cell preservation solution. (whdsbio.cn)
In summary, the composition and use of virus transport media and cell preservation solution are completely different, and Desheng does not produce cell preservation solution. (whdsbio.cn)
Cell2
Deferoxamine improved cell survival and preserved metabolic function in modified Krebs-Henseleit buffer as well as in RPMI 1640. (biomedcentral.com)
The so-called cell preservation solution is a general-purpose cell cryopreservation solution. (whdsbio.cn)
Preservation1
In transplantation medicine, special preservation solutions with often unphysiological, i.e. intracellular ion compositions are used for tissue and organ preservation during extracorporal cold storage, for example University of Wisconsin (UW) solution [ 19 ]. (biomedcentral.com)
Prepare1
Prepare a Calcein UltraGreen™ AM working solution of 1 to 10 µM in the buffer of your choice (e.g. (aatbio.com)
Commonly1
It is most commonly used as a sterile 9 g of salt per litre (0.9%) solution, known as normal saline. (wikipedia.org)
Water1
A 10% v/v solution in water of formalin( a 37% solution of formaldehyde and water). (hl7.org)
Interface1
The mononuclear layer is visible as a white cloudy layer situated at the interface between the two solutions. (mdanderson.org)
Remove3
4. Remove the culture medium, and add 0.5 ml of the Fura 2-AM working solution to the cells. (dojindo.com)
Then remove the Fura 2-AM working solution. (dojindo.com)
Replace the dye working solution with HHBS or buffer of your choice (containing an anion transporter inhibitor, such as 1 mM probenecid, if applicable) to remove any excess probes. (aatbio.com)
Stock1
Note that volume is only for preparing stock solution. (aatbio.com)
Cold1
Immediately fill tubes up to 50 mL with cold 1X lysing solution. (mdanderson.org)