It is shown here for p21ras, a well studied example of GTP hydrolysing proteins, that the GTP-hydrolysis rate is significantly faster if Mg2+ is replaced by Mn2+, both in the presence or absence of its GTPase-activating protein Ras-GAP. (rcsb.org)
Nucleotide5
Unexpectedly, the postfusion conformation of the soluble domain was achieved when bound to the nonhydrolyzable GTP analogue guanosine 5′-[β,γ-imido]triphosphate, suggesting that nucleotide hydrolysis might not be required for the prefusion to postfusion conformational change. (ox.ac.uk)
The small-G-protein family consists of numerous proteins with varying degrees of homology, one of which is a guanosine nucleotide-binding protein (RAS) [ 4 ] . (encyclopedia.pub)
The term nucleotide refers to the building blocks of DNA (deoxyribonucleoside triphosphate, dNTP) and RNA (ribonucleoside triphosphate, NTP). (rainis.pics)
This reaction involves chemically attacking a substrate of EF-Tu, the molecule guanosine-triphosphate (GTP), with water, breaking a bond and turning GTP into guanosine-diphosphate (GDP). (illinois.edu)
The GAP of ARD1 stimulates GTPase hydrolysis for ARD1 but not ARFs. (embl.de)
We then studied the intrinsic dynamin GTPase mechanism by using a combination of fluorescence stopped-flow and HPLC methods using the fluorescent analogue of GTP, mantdGTP (2'deoxy-Y-O-(N-methylanthraniloyl) guanosine-5'-triphosphate), under the same ionic strength conditions. (elsevierpure.com)
A conserved glutamine serves a critical role in this pivotal guanosine triphosphatase (GTPase) reaction. (tau.ac.il)
The normal rate of Ras catalytic GTPase (GTP hydrolysis) activity can be increased by proteins of the RasGAP family, which bind to Ras and increase its catalytic rate by a factor of one thousand - in effect, increasing the rate at which Ras is inactivated. (bionity.com)
Endoplasmic reticulum (ER) network branching requires homotypic tethering and fusion of tubules mediated by the atlastin (ATL) guanosine triphosphatase (GTPase). (ox.ac.uk)
Protein synthesis on the ribosome requires translational GTPase factors to bind to the ribosome in the GTP-bound form, take individual actions that are coupled with GTP hydrolysis, and dissociate, usually in the GDP-bound form. (utmb.edu)
Nucleoside2
Nucleoside (analogue) triphosphate levels were determined by LC-MS/MS. CNDAC interaction with SAMHD1 was analysed by an enzymatic assay and by crystallisation. (biomedcentral.com)
Specifically, the ITPase catalyzes the hydrolysis of (deoxy) nucleoside triphosphates into the corresponding nucleoside monophosphate with the concomitant release of pyrophosphate. (ectrx.org)
that is, these drugs inhibit physiological processes by replacing the natural counterparts (guanosinenucleotides). (ectrx.org)
6,7 Inosine triphosphate pyrophosphates (ITPases) represent a ubiquitous group of protective enzymes encoded by the ITPA gene and function to prevent the incorporation of noncanonical (so-called rogue) purine nucleotides into DNA and RNA. (ectrx.org)
Studies have shown that the accumulation of unusual guanosinenucleotides, collectively called (p)ppGpp, is the hallmark of the stringent response of E. coli [ 15 - 18 ] (Figure 1 ). (biomedcentral.com)
Triphosphatase1
Thus, the guanosine triphosphatase dynamin-1 is indispensable for vesicle endocytosis at this fast central nervous system (CNS) synapse. (fujita-hu.ac.jp)
After GTP hydrolysis, UreG changes its shape and releases its bound nickel ions, activating the urease with the help of UreF/UreH. (edu.hk)
UreG interacts with UreE when GTP is bound, but binds UreF/UreH after GTP hydrolysis. (edu.hk)
Hydrolysis of GTP, bound to members of the G-protein superfamily, terminates their downstream signaling activity. (tau.ac.il)
Nonhydrolyzable1
Presynaptic loading of the nonhydrolyzable guanosine 5′-triphosphate (GTP) analog GTPγS or dynamin-1 proline-rich domain peptide abolished endocytosis. (fujita-hu.ac.jp)
Tubulin1
In other words, the components can assemble, but also react (i.e., tubulin is also an enzyme that hydrolyses guanosine triphosphate GTP), which in turn affects the assemblies. (uni-freiburg.de)
Elongation factor1
Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis. (illinois.edu)
The off-switch is maintained by hydrolysis of GTP to GDP and a phosphate moiety. (biophysics.org)
Protein2
We also show that PilA has a strict specificity for GTP, and that GTP hydrolysis follows first order kinetics, with a maximum velocity (V(max)) of 1900 pmol of P(i) produced per min per mg of protein and a K(m) for GTP of 9.6 μM at 37 °C. (arizona.edu)
A protease can only act on certain peptide bonds in protein molecules, such as the peptide bonds formed by the hydrolysis of basic amino acids catalyzed by trypsin. (glpbio.com)
Enzyme1
Inosine triphosphate pyrophosphatase is an enzyme encoded by the ITPA gene. (ectrx.org)
Adenosine1
Among other things, adenosine is used for autoregulation in the event of an imminent lack of energy in the cell (e.g. when the cell's performance is overloaded or when there is a lack of oxygen): If the ATP content in a cell drops, more adenosine is produced as a hydrolysis product. (adxs.org)
Assays2
In vitro assays using the soluble domain revealed that the salt bridge was dispensable for GTP binding and hydrolysis but was required for forming the postfusion dimer. (ox.ac.uk)
In phosphatidylinositol hydrolysis assays, muscarinic agonists showed greater potency at the HM 1 (S388Y) and HM 1 (S388Y, T389P) mutants compared with the wild-type and HM 1 (T389P) receptors. (aspetjournals.org)
Using ARD1 truncations, it appears that amino acids 101-190 are critical for GAP activity, whereas residues 190-333 are involved in physical interaction between the two domains of ARD1 and are required for GTP hydrolysis. (embl.de)
Recent studies have shown that pathological mutations of LRRK2 can reduce the rate of guanosine triphosphate (GTP) hydrolysis, increase kinase activity and GTP binding activity, and subsequently cause cell death. (biomedcentral.com)
This step required the hydrolysis of GTP associated to ARF. (embl.de)
Factors1
It is likely that the conserved C terminus of the stalk proteins of archaea and eukaryotes can bind to translation factors both before and after GTP hydrolysis. (utmb.edu)
Role1
However, the role of the catalytic glutamine in GTP hydrolysis is still not well understood. (tau.ac.il)