• Enzymatic
  • Because 2-FL can be synthesized through the enzymatic fucosylation of lactose with guanosine 5′-diphosphate (GDP)- l -fucose by α-1,2-fucosyltransferase (FucT2), an 2-FL producing Escherichia coli can be constructed through overexpressing genes coding for endogenous GDP- l -fucose biosynthetic enzymes and heterologous fucosyltransferase. (biomedcentral.com)
  • Pathway
  • The pioneering studies of Ginsburg indicated that GDP- l -fucose is formed by a de novo pathway starting from GDP- d -mannose (Ginsburg 1960, 1961). (springer.com)
  • acid
  • The incorporation of exogenous [ 3 H]dolichol phosphate into the mannolipid, and its release, accompanied by mannose, on treatment of the mannolipid with dilute acid, confirmed that exogenous dolichol phosphate can act as an acceptor of mannose in this system. (biochemj.org)