The researchers used the algorithm to identify split sites on a number of proteins, including tyrosine kinase Lyn, guanosine nucleotide dissociation inhibitor, and guanine exchange factor. (genengnews.com)
We next sought to gain mechanistic insight into actin-independent polarization, which requires both the guanine nucleotide dissociation inhibitor (GDI) Rdi1, which extracts Cdc42 from the peripheral membrane into a rapidly diffusing cytosolic complex, and the adaptor molecule Bem1, which binds both active Cdc42GTP and its guanine nucleotide exchange factor (GEF) or activator Cdc24. (ku.edu)
The molecule, Rho guanine nucleotide dissociation inhibitor α (RhoGDIα), slowed down muscle glucose uptake and hindered insulin's effect. (growkudos.com)
cDNA cloning of component A of Rab geranylgeranyl transferase, analogous to Rab 3A guanine nucleotide dissociation inhibitor (GD1), forms a complex with Rab 1A. (readabstracts.com)
We have purified and characterized several GDP/GTP exchange proteins (GDP dissociation stimulator (GDS) and GDP dissociation inhibitor (GDI)) and GTPase activating Proteins (GAP) for small G proteins. (nii.ac.jp)
Stimulator1
Ral guanine nucleotide dissociation stimulator is a protein that is encoded by the RALGDS gene in humans. (wikipedia.org)
Two classes of intracellular proteins act as inhibitors of G protein activation: GTPase activating proteins (GAPs), which enhance GTP hydrolysis and guanine dissociation inhibitors (GDIs), which inhibit GDP dissociation. (embl-heidelberg.de)
This process is usually regulated by guanine nucleotide-exchange factors GTPase-activating proteins and guanine nucleotide-dissociation inhibitors (1). (sciencepop.org)
Regulated by guanine nucleotide exchange factors (GEFS) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (gaps) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. (lu.se)
GEFs4
Guanine nucleotide exchange factors ("GEFs") stimulate the dissociation of the GTP hydrolytic product, GDP, from small GTP-binding proteins, to promote the binding of a new GTP molecule. (justia.com)
The exchange of GDP to GTP and thus the activation of Rho GTPases is catalyzed by guanine nucleotide exchange factors (GEFs), which act downstream of numerous growth factor receptors, integrins, cytokine receptors, and cadherins. (biomedcentral.com)
Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state. (umbc.edu)
The guanine nucleotide exchange factor (GEF) Dbl targets Rho family proteins thereby stimulating their GDP/GTP exchange, and thus is believed to be involved in receptor-mediated regulation of the proteins. (embl-heidelberg.de)
Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. (icr.ac.uk)
GTP binding causes dissociation of the heterotrimer, liberating the individual subunits so that they can interact with downstream effector proteins. (icr.ac.uk)
Alkali and alkaline earth metal adducts of a branched glycan, XXXG, were analyzed with helium charge transfer dissociation (He‐CTD) and low‐energy collision‐induced dissociation (LE‐CID) to investigate if metalation would impact the type of fragments generated and the structural characterization of the analyte. (nsf.gov)
Results demonstrate that He-CTD is compatible with UHPLC timescales and provides unambiguous glycosidic and cross-ring cleavages from both the reducing and the nonreducing ends, which is not typically possible using collision-induced dissociation. (nsf.gov)
Interpretation of high energy collision induced dissociation mass spectra, along with ultraviolet/visible and fluorescence spectrophotometry allowed the identification of N2-(2- hydroxy-1-phenylethyl)-O6-(2-hydroxy-2-phenylethyl)-2'- deoxyguanosine 3'-phosphate, N2-(2-hydroxy-1-phenylethyl)-O6-(2- hydroxy-1-phenylethyl)-2'-deoxyguanosine 3'-phosphate, and N1,N2- bis(2-hydroxy-1-phenylethyl)-2'-deoxyguanosine-3'-phosphate. (cdc.gov)
GTPases4
Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes. (cancerindex.org)
In cell signalling , Son of Sevenless , or SOS , is a gene encoding a guanine nucleotide exchange factor that acts on Ras - GTPases . (bionity.com)
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. (embl-heidelberg.de)
Inhibit1
The present invention relates to diagnosing abnormal cell proliferation in biological samples and screening for drugs which inhibit, reduce or abolish cell growth, especially tumorigenic cell growth, by detecting a phosphovariant isoform of a guanine nucleotide exchange factor biomarker, such as the novel GEF-H1S. (justia.com)
Subunits1
It is postulated that the mechanism by which Mg2+ and receptors facilitate N activation involves dissociation of n alpha activated ADP-ribosylatable subunits (with guanine nucleotide bound to them) from n beta non-ADP-ribosylatable subunits (with receptor and Mg2+ bound to them). (nih.gov)
Nucleotides1
Monovalent cations (Na+, Li+, and Cs+), divalent cations (Mg2+, Ca2+, and Mn2+), and guanine nucleotides did not significantly affect the specific binding of [3H]ICI-198615 to guinea pig lung LTD4 receptors. (aspetjournals.org)
Inhibits2
this inhibits guanine nucleotide exchange and GTP binding (PubMed:11121039). (icr.ac.uk)
this inhibits guanine nucleotide exchange (By similarity). (icr.ac.uk)
Binds1
Once released from SOS, the Ras-GTPase quickly binds fresh guanine nucleotide from the cytosol. (bionity.com)
Membranes1
GBF1 is phosphorylated by CDK1-cyclin B in mitosis, which results in its dissociation from Golgi membranes. (aston.ac.uk)
Cdc421
There have been limited successes with molecules that disrupt the binding of guanine nucleotide exchange factors to RAC and CDC42 (7-10) as well as with molecules that disrupt GTPase membrane association (11). (sciencepop.org)
Effector1
Guanine nucleotide exchange factor (GEF) for Ral-A. Potential effector of GTPase HRas and Ras-related proteinM-Ras. (novusbio.com)
Ultraviolet2
He-CTD produces informative fragments, including 0,3An and 0,4An ions, which have been observed with electron transfer dissociation, electron detachment dissociation, and ultraviolet photodissociation (UVPD) and are crucial for differentiating the α-2,3- versus α-2,6-linked sialic acid (Neu5Ac) residues present among sialyllacto-N-tetraose HMOs. (nsf.gov)
The other most abundant adducts represented about 65% of the total covalent binding and were identified as depurinated N7-substituted guanines using tandem mass spectrometry with ultraviolet/visible spectroscopy. (cdc.gov)
Regulatory1
Activation of metabotropic glutamate receptor signaling triggered transient dissociation of eEF1A2 from its regulatory guanine exchange factor (GEF) protein in dendritic spines in a phosphorylation-dependent manner. (ibecbarcelona.eu)
Exchange factor2
Activation of Rab39A is catalyzed by C9orf72, a guanine exchange factor associated with amyotrophic lateral sclerosis and familial frontotemporal dementia. (bvsalud.org)
Structure of Shigella IpgB2 in complex with human RhoA: implications for the mechanism of bacterial guanine nucleotide exchange factor mimicry. (helmholtz-hzi.de)
Activation2
These experiments indicate that the glucagon receptor facilitates activation of N by: (i) decreasing the apparent Km of N for Mg2+, and (ii) increasing the extent of activation that can be elicited by saturating concentrations of guanine nucleotide. (nih.gov)
such observations are similar to previous reports for magnesium and calcium salts undergoing electron capture dissociation (ECD) activation. (nsf.gov)
Bound1
The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. (icr.ac.uk)
Structural2
These three were studied and their structural nature presented as isomeric bis substituted guanine mononucleotides. (cdc.gov)
ABSTRACT: The structural stability of guanine quadruplexes depends critically on an unusual configuration of dehydrated Na+ or K+ ions, closely spaced along the central axis of the quadruplex. (lu.se)
MeSH1
Guanine Nucleotide Exchange Factors" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ouhsc.edu)
Alpha2
The alpha chain contains the guanine nucleotide binding site (PubMed:8521505, PubMed:24596087, PubMed:25037222). (icr.ac.uk)
We also show that dissociation of the GDP.Mg(2+) complex is preceded by the displacement of the metal ion to the alpha-phosphate of the nucleotide, diminishing its affinity to the GTPase. (helmholtz-hzi.de)
Mass1
Abstract The combination of helium charge transfer dissociation mass spectrometry (He-CTD-MS) with ultrahigh performance liquid chromatography (UHPLC) is presented for the analysis of a complex mixture of acidic and neutral human milk oligosaccharides (HMOs). (nsf.gov)
Constant2
The dissociation constant (KD) and the density (Bmax) were 0.08 +/- 0.04 nM and 1030 +/- 180 fmol/mg, respectively. (aspetjournals.org)
The binding affinity of an SH2 domain to a pTyr containing ligand is moderate, with the typical affinity range between 0.1 µМ to 10 µМ for equilibrium dissociation constant values (Kd) ( Kaneko,2012 ). (eu.org)
Total1
This graph shows the total number of publications written about "Guanine Nucleotide Exchange Factors" by people in this website by year, and whether "Guanine Nucleotide Exchange Factors" was a major or minor topic of these publications. (ouhsc.edu)