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  • kinase
  • Cavin S, Maric D, Diviani D. A-kinase anchoring protein-Lbc promotes pro-fibrotic signaling in cardiac fibroblasts. (springer.com)
  • Gα12 stimulates c-Jun NH 2 -terminal kinase through the small G proteins Ras and Rac. (springer.com)
  • proteins belonging to all 4 subfamilies, including G s , G i , G q , and G 12 are found to play important roles in receptor tyrosine kinase signaling, regulation of oxidant production, development, and cell migration, through physical and functional interaction with proteins other than 7TMRs. (aspetjournals.org)
  • DAG activates protein kinase C PKC-epsilon , that phosphorylates the cytoplasmatic tail of the beta-1 integrin subunit ( ITGB1 ) . (pathwaymaps.com)
  • Tyrosine-protein kinase c-Src is activated by G-protein alpha-q/11 , G-protein alpha-12 family , and G-protein beta/gamma subunits. (pathwaymaps.com)
  • In most cell types, c-Src stimulation is involved in GPCR-mediated activation of the Focal adhesion kinase FAK1 and the mitogen-activated protein kinases ERK1 / 2 . (pathwaymaps.com)
  • Silencing Ras-Related C3 Botulinum Toxin Substrate 1 Inhibits Growth and Migration of Hypopharyngeal Squamous Cell Carcinoma via the P38 Mitogen-Activated Protein Kinase Signaling Pathway. (nih.gov)
  • Canonical apelin-stimulated APJ signaling involves activation of pertussis toxin-sensitive G-proteins (G i or G o ) leading to reduced cAMP production, as well as the activation of phospholipase C, protein kinase C, and the extracellular signal-regulated kinases 1 and 2. (ahajournals.org)
  • 4 Apelin induces an inotropic response in cardiomyocytes by increasing the amplitude of the [Ca] 2+ i transient in a protein kinase C-dependent manner. (ahajournals.org)
  • Canonical apelin-dependent APJ signaling via Gα i and Gα q leads to the activation of protein kinase C (PKC) and phosphatidylinositide 3-kinase (PI3K) and the inhibition of adenylyl cyclase (AC). (ahajournals.org)
  • LRRK2 encodes a large (2,527-aa) multidomain protein originally identified as a unique kinase with leucine-rich repeats. (pnas.org)
  • LRRK2 is a member of a superfamily of proteins, named ROCO, that includes at least three other human proteins: leucine-rich repeat kinase 1 (LRRK1), death-associated protein kinase (DAPK1), and malignant fibrous histiocytoma-amplified sequences with leucine-rich tandem repeat-1 (MASL1) ( 3 ). (pnas.org)
  • Both LRRK2 and LRRK1 have been shown to be active protein kinases in vitro ( 4 - 7 ), and some mutations are found in the kinase domain. (pnas.org)
  • However, the kinase activity of LRRK2 is required for the ability of the mutant protein to cause neuronal damage, at least in cell culture models ( 5 , 10 ), suggesting that kinase inhibitors may represent a therapeutic avenue for PD. (pnas.org)
  • Although the kinase domain therefore is important in understanding pathogenesis, mutations also are found in other regions of the protein, and understanding why these mutations cause disease is difficult. (pnas.org)
  • Several studies have shown that GTP binding at the ROC domain regulates kinase activity ( 4 , 7 , 10 , 15 , 16 ). (pnas.org)
  • This finding suggests that mutations outside of the kinase domain may indirectly impact enzyme function but leave open the question of how these mutations affect the structure of the protein and, in turn, how this impacts communication between the GTP-binding region and the kinase domain. (pnas.org)
  • pathway
  • Regions on adenylyl cyclase VII required for selective regulation by the G13 pathway. (springer.com)
  • PKC-epsilon is also activated in G-protein alpha-12 family signaling pathway . (pathwaymaps.com)
  • this pathway is blocked by UNC‐13 mutants unable to bind DAG. (embopress.org)
  • The findings uncovered a critical HGF-dependent signaling pathway that involves the assembly of a large protein complex consisting of MET, AXL, ELMO2, and DOCK180 on the plasma membrane, leading to RAC1-dependent cell migration and invasion in various cancer cells. (nih.gov)
  • GTPases
  • The structure of the LRRK2 ROC domain also represents a signature from a previously undescribed class of GTPases from complex proteins and results may provide a unique molecular target for therapeutics in PD. (pnas.org)
  • cognate
  • We essentially focused on our recent studies in which we addressed the concept of preassembly vs. the agonist-dependent interaction between the protease-activated receptor 1 (PAR1) and its cognate G proteins. (frontiersin.org)
  • RhoA
  • Expression of active Gα 13 increased GTP-RhoA and GTP-Rac1 levels, whereas expression of active Gα q increased GTP-Rac1 levels only. (nih.gov)
  • psychomotor protein between p75NTR and RHOA syndrome has to disorder of RHOA type and cytoskeletal monophosphate. (erik-mill.de)
  • GPCR
  • As a result, new concepts have emerged, but few questions are still a matter of debate illustrating the complexity of GPCR-G protein interactions and coupling. (frontiersin.org)
  • Here, we summarized the recent advances on our understanding of GPCR-G protein coupling based on BRET approaches and supported by other FRET-based studies. (frontiersin.org)
  • conformational
  • We discussed the concept of agonist-induced conformational changes within the preassembled PAR1-G protein complexes as well as the critical question how the multiple coupling of PAR1 with two different G proteins, Gαi1 and Gα12, but also β-arrestin 1, can be regulated. (frontiersin.org)
  • Talin binding to integrin beta-1 ( ITGB1 ), integrin beta-2 (ITGB2 ), integrin beta-3 ( ITGB3 ) cytoplasmic tails induces conformational changes in their extracellular domains, increasing integrin affinity for ligands. (pathwaymaps.com)
  • The binding of PtdIns(4,5)P2 to Talin induces a conformational change that enhances its association with integrin beta subunits. (pathwaymaps.com)
  • microtubule
  • Discovery of these novel GAP, GDI, and GEF activities have helped to illuminate a new role for Gα subunit GDP/GTP cycling required for microtubule force generation and mitotic spindle function in chromosomal segregation. (ijbs.com)
  • novel
  • In recent years, novel accessory proteins involved in the activation of G proteins, either by directly influencing GTP binding or through their association with Gα or Gβγ subunits, have been uncovered ( Sato and Ishikawa, 2010 ). (aspetjournals.org)
  • With a focus on identifying any other novel regulatory protein(s) that can directly interact with Gα13, we subjected Gα13 to tandem affinity purification-coupled mass spectrometric analysis. (jmolecularsignaling.com)
  • gene
  • They are encoded by the largest gene family in the mammalian genomes and they constitute the site of binding and action of a large panel of natural mediators such as hormones and neurotransmitters ( Bockaert and Pin, 1999 ). (frontiersin.org)
  • regulation
  • This Minireview aims to summarize our current understanding of the noncanonical roles of Gα proteins in cell signaling and to discuss unresolved issues including regulation of Gα activation by proteins other than the 7TMRs. (aspetjournals.org)
  • roles
  • Here, we review more recent discoveries that have highlighted newly-appreciated roles for RGS proteins beyond mere negative regulators of 7TM signaling. (ijbs.com)
  • active
  • By contrast, active Galpha(13) and Galpha(q), but not Galpha(s), slightly increased GTP-bound Rac1 levels. (nih.gov)
  • mutant
  • We show here that a non‐DAG‐binding‐UNC‐13 mutant that partially blocks increased ACh release by activated RHO‐1 completely blocks increased ACh release by activated GPA‐12. (embopress.org)