• Therefore, the channel-activating effectiveness of these subunits on isolated guinea pig atrial cells was tested. (sciencemag.org)
  • Signaling governed by G proteins and cAMP is crucial for growth, secondary metabolism and sexual development in Fusarium fujikuroi. (nih.gov)
  • Here we studied the impact of the heterotrimeric G protein and the cAMP-mediated signaling network, including the regulatory subunits of the cAMP-dependent protein kinase (PKA), to study their effect on colony morphology, sexual development and regulation of bikaverins, fusarubins and GAs. (nih.gov)
  • Abdollahi MR et al (2009) Mutation of the variant alpha-tubulin TUBA8 results in polymicrogyria with optic nerve hypoplasia. (springer.com)
  • Development of procedures to knock out the function of intracellular proteins by expression of cDNAs encoding specific antibodies. (nii.ac.jp)
  • 7. Insertion of immunoglobulin heavy and light chain cDNAs into expression vectors that contain an inducible promoter derived from mouse mammary tumor virus or Drosophila heat-shock protein 70 gene. (nii.ac.jp)
  • We found that endothelial VEGFR-2 expression is strongly reduced in the absence of the G protein G13, resulting in an impaired responsiveness to VEGF-A, a phenotype that can be rescued by normalization of VEGFR-2 levels. (mpg.de)
  • G13-mediated VEGFR-2 expression involved activation of the small GTPase RhoA and transcription factor NF-kappaB, the latter acting via a specific binding site at position -84 of the VEGFR-2 promoter. (mpg.de)
  • Taken together, we identified G-protein-mediated signaling via G13 as a critical regulator of VEGFR-2 expression during angiogenesis. (mpg.de)
  • Compared to control, expression of TGR5 and Gα s (but not Gα i and Gα q ) proteins was increased 2-fold to 3-fold in cystic cholangiocytes in vitro and in vivo. (elsevier.com)
  • Stable cell lines with equivalent transfected Gsα protein expression that had relatively lower (WT) or higher (R201C and R201H) cAMP levels were generated. (nih.gov)
  • Our results suggest that point mutations in the signature sequence of the A (Val 49) and C (Thr 225) homologous regions that are implicated in regulating the GTPase activity of the molecule also resulted in the activation of the subunit. (umassmed.edu)
  • These results demonstrate that AA metabolites from cPLA 2 activation contribute to Lkt-induced cytolysis and G i type G-proteins, Ca 2+ and PKC, regulate the cPLA 2 activity. (umn.edu)
  • Activation is terminated by a GTPase intrinsic to the G-alpha subunit. (wikipedia.org)
  • In contrast to the action of insulin on GLUT4, the effects of endothelin 1 and G(alpha)11 were not inhibited by the phosphatidylinositol 3-kinase inhibitor wortmannin at 100 nM. (umassmed.edu)
  • Signaling by insulin, endothelin 1, or G(alpha)11(Q209L) also mobilized cortical F-actin in cultured adipocytes. (umassmed.edu)
  • These data indicate that ARF6 is a required downstream element in endothelin 1 signaling through G(alpha)11 to regulate cortical actin and GLUT4 translocation in cultured adipocytes, while insulin action involves different signaling pathways. (umassmed.edu)
  • We examined the possibility of culturing muscle cells of gilthead sea bream in vitro and assessed variations in insulin-like growth factor-I (IGF-I) binding during myocyte. (naver.com)
  • GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). (genecards.org)
  • G(alpha)11 signaling through ARF6 regulates F-actin mobilization and G" by Avirup Bose, Andrew D. Cherniack et al. (umassmed.edu)
  • We have also generated point mutations in the Gαs subunit that are comparable to the activating mutations in the ras protein. (umassmed.edu)
  • Mis-sense mutations in the α-subunit of the G-protein, Gsα, cause fibrous dysplasia of bone/McCune-Albright syndrome. (nih.gov)
  • 14-3-3 proteins are phosphoserine/phosphothreonine-recognizing adapter proteins that regulate the activity of a vast array of targets. (naver.com)
  • These results support the view that, physiologically, the alpha subunit activates the channel. (sciencemag.org)
  • Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG) (PubMed:15240885, PubMed:12515866). (genecards.org)
  • We predicted that appropriate genetic manipulation of key regions of the α-subunit could result in the induction of the active conformation that would mimic at least in part the activated GTP-bound state. (umassmed.edu)
  • Mitogen-activated protein kinase (MAPK) is modulated by auxin and the protein kinase PINOID has a role in auxin transport modulation even though their functional linkage to other signalling molecules is ill-defined. (springer.com)
  • C/EBP alpha knock-in hepatocytes exhibit increased albumin secretion and urea production. (naver.com)
  • The relationship between the binding affinity and specificity of modular interaction domains is potentially important in determining biological signaling responses. (naver.com)
  • Commercially available Chinese hamster ovary cells were stably transfected with either wild-type (WT) or mutant Gsα proteins (R201C and R201H). (nih.gov)
  • Overexpression of auxin-binding protein enhances the sensitivity of guard cells to auxin. (springer.com)
  • In addition, we provide evidence that G Protein-mediated/cAMP signaling is important for growth in F. fujikuroi because deletion of ffg3, ffac and ffpka1 resulted in impaired growth on minimal and rich media. (nih.gov)
  • These alpha-subunits (designated as GL1alpha and GL2alpha) lack apparent ADP-ribosylation sites for pertussis toxin. (nii.ac.jp)