Growth hct116 cells. Medical search. Frequent questions

The proliferation of HCT116 colonies was found to be inhibited by 5-Fu/P85 copolymer micelles. (wikipedia.org)
HCT116 cells are used in a variety of biomedical studies involving colon cancer proliferation and corresponding inhibitors. (wikipedia.org)
HCT116 cell proliferation was assessed by conducting crystal violet and BrdU assays. (biomedcentral.com)
EOPK significantly decreased HCT116 cell proliferation and migration, and induced G1 arrest without affecting normal cells. (biomedcentral.com)
Our studies indicate that EOPK significantly reduced proliferation and migration of colorectal cancer cells. (biomedcentral.com)
PAK1 is an important effector of Rac and Cdc42 that regulates cell transformation and tumor proliferation [ 9 ]. (biomedcentral.com)
New anticancer therapy strategies refer to the inhibition of CDK-cyclin complexes as an important target to prevent uncontrolled proliferation and induce apoptosis in cancer cells ( 2 ). (spandidos-publications.com)
Natural polyamines, putrescine, spermidine and spermine, play essential roles in the regulation of cell growth and proliferation. (spandidos-publications.com)
Furthermore, we show that the ligands Wnt5a/b are upstream regulators of the non-canonical signature and moreover regulate proliferation of cancer cells in a β-catenin-independent manner. (nature.com)
Our experiments indicate that colon cancer cells are dependent on both β-catenin-dependent and -independent Wnt signaling routes for growth and proliferation. (nature.com)
Therefore, this study proves that Rh4 inhibits cancer cell proliferation by activating the ROS/p53 signaling pathway and activating autophagy to induce ferroptosis, which provides necessary scientific evidence of the great anticancer potential of Rh4. (hindawi.com)
Transcriptome and chromatin immunoprecipitation analysis revealed c-Myc as a new and direct downstream target of XBP1 for regulation of NK cell proliferation. (cancerindex.org)
Our results show a novel role for DDX5 in cancer cell proliferation and suggest DDX5 as a therapeutic target in breast cancer treatment. (aacrjournals.org)
DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
Defects in the control of cell proliferation are a hallmark of cancer, and DNA replication is a key process for cell proliferation. (aacrjournals.org)
Curcumin, a natural polyphenolic compound derived from turmeric (Curcuma longa L), has proven to be a modulator of multiple intercellular signalling pathways linked to inflammation, to proliferation, growth, invasion, drug sensitivity, angiogenesis and metastasis of cancer cells. (researchgate.net)
In vitro, it was found that adiponectin directly controls malignant potential (cell proliferation, adhesion, invasion and colony formation) and regulates metabolic (AMPK/S6), inflammatory (STAT3/VEGF) and cell cycle (p21/p27/p53/cyclins) signalling pathways in both mouse MCA38 and human HT29, HCT116 and LoVo colon cancer cell lines in a LKB1-dependent way. (nuthealth.org)
d) Colony formation assay validates G3P-induced suppressive proliferation in HCT116 cells. (bioz.com)
m) Proliferation of the control and ENO1 knockdown cells under G3P treatment (n = 5). (bioz.com)
Reducing cancer proliferation and promoting cell death. (pall.com)
The inability to utilize butyrate leads to a buildup inside the CRC cells, particularly within the nucleus where it acts as a histone deacetylase inhibitor and works to suppress CRC cell proliferation while promoting apoptosis. (pall.com)
demonstrated the role and mode of action of the beneficial gut bacteria L.plantarum in increasing the sensitivity of 5-FU resistant CRC cells to chemotherapy, as well as reducing proliferation, and inducing apoptosis in the cancerous cells. (pall.com)
Many physiological processes, including proper tissue development and homeostasis, require a balance between apoptosis and cell 123653-11-2 manufacture proliferation. (bioinf.org)
Apoptosis and cell proliferation are linked by cell-cycle regulators and apoptotic stimuli that affect both processes. (bioinf.org)
A failure in regulating proliferation together with suppression of apoptosis are the 123653-11-2 manufacture minimal requirements for a cell to become cancerous [4]. (bioinf.org)
Two proteins involved intimately in regulating cell proliferation are Akt and the tumor suppressor p53 (p53). (bioinf.org)
p53 has an important protective role against undesired cell proliferation. (bioinf.org)
Sirtuins are NAD + -dependent histone deacetylases regulating important metabolic pathways in prokaryotes and eukaryotes and are involved in many biological processes such as cell survival, senescence, proliferation, apoptosis, DNA repair, cell metabolism, and caloric restriction. (biomedcentral.com)
catenin signaling and influences CRC cell proliferation and experiments in CRC cell lines and an tumor xenograft model. (immune-source.com)

To determine mechanisms underlying growth promotion by LRH-1 in CRC, we undertook global expression profiling following siRNA-mediated LRH-1 knockdown in HCT116 cells, which require LRH-1 for growth and in HT29 cells, in which LRH-1 does not regulate growth. (dundee.ac.uk)
p21 regulation was not observed in HT29 cells, where p53 is mutated. (dundee.ac.uk)
Results Inhibition of CRC cell growth by oxaliplatin is usually associated with Bexarotene (LGD1069) the presence of wild-type p53 To investigate the cytotoxicity of the anticancer agent oxaliplatin in CRC cells CCK8 assays were performed using HCT116 SW480 and HT29 cells treated with various concentrations of oxaliplatin for 24?h. (immune-source.com)
As shown in Fig. 1A oxaliplatin inhibited cell growth in these three CRC cell lines in a Bexarotene (LGD1069) dose-dependent manner with HCT116 cells being more sensitive to oxaliplatin than SW480/HT29 cells (Fig. 1A). (immune-source.com)
In addition p53 expression was high in HCT116 cells and lower in SW480/HT29 cells (Fig. 1C). (immune-source.com)
HRT18 and HT29 cell lines exhibited lack of imprinting (LOI), while HCT116 and ASPC taken care of regular imprinting (MOI) [31C33]. (woofahs.com)
Using limitation enzyme keying in and DNA sequencing of genomic DNA (gDNA), six individual cancers cell lines had been divided into beneficial (heterozygous C/T) and non-informative (homozygous C/C). By evaluating the appearance of cDNA, HRT18 and HT29 had been proven to demonstrate lack of imprinting (LOI). (woofahs.com)

Many studies on the cancer-killing activity of 125 I seeds radiation mainly focused on its ability to trigger apoptosis, which is the most well-known and dominant type of cell death induced by radiation. (hindawi.com)
However our results showed some unique morphological features such as cell swelling, cytoplasmic vacuolation, and plasma membrane integrity, which is obviously different to apoptosis. (hindawi.com)
We found besides apoptosis that 125 I seed radiation killed colorectal cancer cell via inducing paraptosis. (hindawi.com)
This form of cell death is fundamentally different from apoptosis and lacks some distinct characteristics of apoptosis such as DNA fragmentation, pyknosis, or caspase activation and cleavage [ 14 ]. (hindawi.com)
Although purvalanol-induced cell cycle arrest and apoptotic cell death were demonstrated in prostate ( 5 ), breast ( 6 ) and colon cancer cells ( 7 ), the exact molecular mechanism of purvanol-induced apoptosis has not been elucidated yet. (spandidos-publications.com)
Activation of TRPV1 by capsaicin caused significant inhibition of cancer cell growth and induced apoptosis and necrosis. (dovepress.com)
10 TRPV1 agonist, capsaicin, is capable of inducing apoptosis 11 , 12 and inhibiting cancer cell growth by cell cycle arrest in many different types of cancer, for example, osteosarcoma, colon, and pancreatic cancer cells, while normal cells remained unharmed. (dovepress.com)
The authors utilized cultured L. plantarum cells to generate a cell-free supernatant that could be used in experiments on cultured HCT116/5FU cells to determine levels of apoptosis, and cellular activity under a range of different conditions including treatment with butyrate and chemotherapy drugs both alone and in combination. (pall.com)
Conclusions These findings ascertained an conversation between p53, c-myc, p21, p27, Bcl-2, PI3K/Akt pathway, and CF-induced apoptosis in MSTO-211H and HCT-116 cells, suggesting that CF acts as an important regulator of cell growth in human malignancy cell lines. (bioinf.org)
Apoptosis (programmed cell death) occurs in a wide variety of physiological NIK settings, where its role is to remove harmful, damaged or unwanted cells. (bioinf.org)
Oxaliplatin causes cell-cycle arrest promotes accelerated senescence and induces apoptosis in Bexarotene (LGD1069) cancer cells7 8 9 The p53 protein is involved in many biological processes the best known of which are cell-cycle arrest and DNA repair10 11 p53 also regulates apoptosis after exposure to hypoxia and cytotoxic drugs and is one of the most commonly mutated genes in many types of cancer12. (immune-source.com)

In this study, stimulation by the TRPV1 agonist, capsaicin, of SUM149PT cells, a model system for the most aggressive breast cancer subtype, triple-negative breast cancer, led to intracellular calcium signals that were diminished by the specific TRPV1 antagonist, capsazepin. (dovepress.com)
a) Intracellular concentrations of G3P in HCT116 cells treated with solvent control, NaF (3 mM) and GNE140 (10 μM) for 24 hr determined by targeted LC/MS-MS (n = 3). (bioz.com)
High KIF20A expression in resistant CRC cell line suppressed the intracellular ferroptosis process. (aging-us.com)

To assess the effects of EOPK on cell migration, we performed a wound-healing assay. (biomedcentral.com)
We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
The assay requires cells to grow in an anchorage independent manner, which is a hallmark of cancer cells. (moleculardevices.com)
In an alternative assay, cells were fixed for 1 hour using a 4% formaldehyde solution, washed 3x with PBS by removing half of the volume each time. (moleculardevices.com)
f) Thermal shift assay validated the ENO1-G3P engagement in HCT116 cell lysates. (bioz.com)
C ) The expression level of KIF20A in different colorectal cancer cell lines were examined by WB assay. (aging-us.com)

HCT116 cells can also function in xenografts, with docetaxel, 5-FU, and flavopiridol limiting tumor growth in vitro. (wikipedia.org)
Further, a recent report on the effects of EOPK indicated that the oil has anti-obesity and hypolipidemic activity in vitro and in vivo , using 3 T3-L1 cells and high fat diet-fed rats [ 16 ]. (biomedcentral.com)
In in vitro studies, the addition of NT to human colon cancer cell lines resulted in significantly increased cancer cell growth and the growth of xenografted human colon cancer cells in mice [ 9 ]. (springer.com)
In-vitro antiplasmodial efficacy of 4,7-dichloroquinoline revealed a significant growth inhibition of both sensitive strains of Plasmodium falciparum with IC 50 values of 6.7 nM (CQ-s) and 8.5 nM (CQ-r). (nature.com)
In vitro activity of doxorubicin-loaded cubosomes was studied on HCT-116 cells. (ijper.org)
A dichloromethane extract of the stem bark of Cryptocarya nigra showed strong in vitro inhibition of Plasmodium falciparum growth, with an IC50 value of 2.82 μg/mL. (afpm.org.my)

p53 dependence for the regulation of p21 by LRH-1 was confirmed by p53 knockdown with siRNA, while LRH-1-regulation of p21 was not evident in HCT116 cells where p53 had been deleted. (dundee.ac.uk)
CYP2S1 knockdown conferred a cell survival advantage after oxaliplatin treatment to cells harboring wild-type p53 and cytotoxicity and antitumor activity. (immune-source.com)

The receptor for epidermal growth factor (EGFR) is overexpressed in many cancers. (aacrjournals.org)
whereas human epidermal growth factor receptor 2 (HER2)-positive breast cancers can be targeted by specific drugs, the triple-negative breast cancer phenotype is defined by chromosomal instability and low expression levels of estrogen receptor (ER) alpha, progesterone receptor (PR), and HER2, rendering treatment rather difficult. (dovepress.com)
Furthermore, butyrate is detrimental to cancerous cells due to the Warburg effect, a well-documented phenomenon common to most cancers wherein the cancerous cells can only derive energy from simple sugars via aerobic glycolysis, or fermentation. (pall.com)
In the context of aberrant growth control, many important genes responsible for the genesis of various cancers have been discovered and the pathways through which they act characterized. (bioinf.org)

Activation of Akt leads to the stimulation of antiapoptotic pathways, promoting cell survival. (aacrjournals.org)
Distinct pathways of cell migration and antiapoptotic response to epithelial injury: structure-function analysis of human intestinal trefoil factor. (embl.de)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The gut microbiota provides a beneficial therapeutic effect by targeting cancer cells through several anti-cancer pathways. (pall.com)

Cellular transformation/tumorigenicity assays using cultures of cells in semi-solid media (soft agar or Matrigel) has been well established for cancer research 1,2,5 . (moleculardevices.com)

Cyclin-dependent kinases (CDKs), are members of the serine-threonine protein kinase family and are responsible for taking control of cell cycle regulation in eukaryotic cells. (spandidos-publications.com)
We demonstrate that LRH-1-mediated p21 regulation in HCT116 cells does not involve altered p53 protein or phosphorylation, and we show that LRH-1 inhibits p53 recruitment to the p21 promoter, likely through a mechanism involving chromatin remodelling. (dundee.ac.uk)
Interestingly, we found circRNA-mediated regulation of target miRNAs and an in vivo growth inhibitory effect upon TUSC3 circ104557 transduction. (oncotarget.com)
CF treatment on leukemia cell lines induces cell death due to apoptotic mechanisms and altering 123653-11-2 manufacture cell metabolism through HIF-1 and GLUT-1 regulation [3]. (bioinf.org)
We have identified a 25-bp sequence at -201 to -225 within the TGFα promoter which mediates the differential regulation of TGFα expression during quiescence establishment in these two cell lines. (elsevierpure.com)

In vivo, NTSR1 mRNA expression was undetectable in superficial differentiated epithelial cells in histological specimens of normal human colonic epithelium, but there was moderate and strong expression in adenomas and adenocarcinomas respectively. (springer.com)
The highly tumorigenic cell line HCT116 up-regulates TGFα mRNA expression during growth arrest, whereas the poorly tumorigenic growth factor-dependent FET cell line down- regulates TGFα mRNA expression as it becomes quiescent. (elsevierpure.com)
The abberant upregulation of TGFα mRNA in quiescent HCT116 cells may allow them to return to the dividing state under more stringent conditions (nutrient replenishment alone) then quiescent FET cells (requires nutrients and growth factors). (elsevierpure.com)
As we previously reported [31C33], both the C and T alleles of mRNA transcripts are present in non-CSCs (middle panel), indicating loss of imprinting in this cancer cell line. (woofahs.com)
B) qRT-PCR results display the mRNA level of hIPI3 fluctuated during the cell cycle and the highest level was between M phase and early G1 phase. (irjs.info)

Paraptosis is a kind of caspase-independent programmed cell death and is characterized by distinct cytoplasmic vacuolization derived from swelling endoplasmic reticulum and/or mitochondria. (hindawi.com)
Furthermore, it was found that the knockout of MARCH2 limited growth of HCT116 cells via stress on the endoplasmic reticulum. (wikipedia.org)
The IRE1 endoplasmic reticulum stress sensor activates natural killer cell immunity in part by regulating c-Myc. (cancerindex.org)
Here, we demonstrate that the endoplasmic reticulum stress sensor inositol-requiring enzyme 1 (IRE1α) and its substrate transcription factor X-box-binding protein 1 (XBP1) drive NK cell responses against viral infection and tumors in vivo. (cancerindex.org)

Genomic DNA was extracted and purified from HCT116 cells using Qiagen QiaAMP Kit according to standard instructions. (nih.gov)
Genomic DNA was extracted and purified from cell pellets using the Qiagen DNeasy Kit according to the manufacturer's instructions. (nih.gov)

HCT116 cells have a mutation in codon 13 of the KRAS proto-oncogene, and are suitable transfection targets for gene therapy research. (wikipedia.org)
When transducted with viral vectors carrying the p53 gene, HCT116 cells remain arrested in the G1 phase. (wikipedia.org)
HCT 116 colon cancer cells were exposed to purvalanol, which activated ER stress via upregulation of PERK, IRE1α gene expression, eIF-2α phosphorylation and ATF-6 cleavage at early time-points in the HCT 116 colon cancer cells. (spandidos-publications.com)
Here, we aimed at identifying a β-catenin-independent Wnt target gene signature in order to understand the functional significance of non-canonical signaling in colon cancer cells. (nature.com)
This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. (cancerindex.org)
Gene ontology (GO) analysis suggested platelet-derived growth factor A (PDGFA) and proto-oncogene, Serine/Threonine kinase Raf-1 (RAF1) as new CRC candidate markers in CTCs and TDEs. (researchsquare.com)
2] The authors showed that the acquisition of butyrate resistance in a common strain of 5-FU resistant CRC cells (HCT116/5FU) was strongly correlated with the inhibition of the expression and function of the SMCT1 gene, which encodes a major transporter protein used to transfer butyrate across the cell membrane and into colonic epithelial cells. (pall.com)
It is this gene that becomes silenced in butyrate insensitive CRC cells, a conclusion that is further supported by previously published studies showing a positive correlation between SMCT1 expression levels in CRC cells with remission and patient survival. (pall.com)

HCT116 is a human colon cancer cell line used in therapeutic research and drug screenings. (wikipedia.org)
In conclusion, the current study revealed the expression profiles of human TRP channels in 60 different breast cancer tissues and cell lines and furthermore validated the antitumor activity of TRPV1 against SUM149PT breast cancer cells, indicating that activation of TRPV1 could be used as a therapeutic target, even in the most aggressive breast cancer types. (dovepress.com)
There is clearly a need for therapeutic strategies in addressing CRC that increase the sensitivity of the tumor cells to chemotherapy compounds, allowing reduced dosing, minimizing side effects, and enabling effective therapy to continue. (pall.com)
Our experiments confirm and extend the involvement of CYP2S1 as a potential therapeutic target for enhancing oxaliplatin efficacy in colorectal epithelial cells. (immune-source.com)

Additionally, EOPK suppressed the expression of PAK1, and decreased ERK and AKT phosphorylation in HCT116 cells. (biomedcentral.com)
Rapamycin could fully inhibit S6 in all cell lines, but this was accompanied by activation of Akt phosphorylation. (aacrjournals.org)

Compared with adherent cells grown in 2D monolayers, 3D growth conditions are believed to more accurately reflect the natural environment of cancer cells and span the gap between 2D cultures and animal studies 3,5 . (moleculardevices.com)

In conclusion, acetone and methanol extracts from C. odontophyllum stem bark exhibited promising cytotoxic activity against human colorectal cancer cell line HCT 116 and paves way for further investigation in view of its mode of cancer cell death. (scirp.org)
Our study aimed to determine the inhibitory and also proliferative effects of a methanol plant extract of Siberan ginseng (E. senticosus) on various cancer and normal cell lines including: A-549 (small cell lung cancer), XWLC-05 (Yunnan lung cancer cell line), CNE (human nasopharyngeal carcinoma cell line), HCT-116 (human colon cancer) and Beas-2b (human lung epithelial). (nih.gov)
Next, in CNE (human nasopharyngeal carcinoma cell line) there was a dose dependent proliferative response, whereas in Beas-2 (human lung epithelial cell line), an inhibitory effect. (nih.gov)
In this study, we selected two transformed human colon cancer cell lines for studies on the role of COX-2 in intestinal tumorigenesis. (nih.gov)
IRE1α-XBP1 were essential for expansion of activated mouse and human NK cells and are situated downstream of the mammalian target of rapamycin signaling pathway. (cancerindex.org)
Understanding how DNA replication is regulated in human cells can provide insight into cancer development and may reveal vulnerabilities that can be exploited therapeutically. (aacrjournals.org)
As test cases to examine changes in protein presence associated with tumor growth inhibition, we applied SILAC-based proteomics on human colon carcinoma cells treated with galectin-4 (Gal-4). (uni-muenchen.de)
By tracing significant alterations of protein expression likely relevant for the observed phenotypic effects, the capacity of a galectin to affect the proteome of human colon cancer cells at multiple sites is revealed. (uni-muenchen.de)
To prepare spheroid cultures in semi-solid media, human colon carcinoma HCT116 cells were pre-mixed with a solution of 4.25 mg/mL cold Matrigel (Corning) diluted in growth media and plated into 96-well half-area plates (Greiner) at a density of \~500 cells/well for a total volume of 25 μL. (moleculardevices.com)
In contrast silencing p53 significantly decreases the inhibitory effects of oxaliplatin suggesting an important role for p53 in this process13 14 The p53 protein regulates a group Bexarotene (LGD1069) of cytochrome P450 (CYP) genes in human and mouse liver cells and influences the efficacy of chemotherapeutic treatment regimens15 16 However a role for p53 in regulating CYP450 genes in the intestinal tract has not yet been reported. (immune-source.com)
For example CYP2S1 which is usually most highly expressed in intestinal tract epithelial cells may be involved in metabolizing aromatic hydrocarbons and other xenobiotic substrates20 21 Madanayake also identified that human CYP2S1 is an important enzyme in the metabolism of COX-derived prostaglandins at nanomolar concentrations and the authors suggested that CYP2S1 may play an important role in modulating the inflammatory process23. (immune-source.com)
It was reported that a human being protein comprising five WD40 repeat domains can stabilize ORC binding to chromatin by interacting with ORC and modulating the chromatin association of ORC in human being cells [12], suggesting that WD40 repeat proteins play important roles in human being DNA replication. (irjs.info)
Here we display that hIPI3 also has important functions in the initiation of DNA replication TMC353121 in human being cells. (irjs.info)

Moreover, the expression of AIP1 is specifically inhibited in paraptosis cells, while it is not affected in apoptotic cells [ 15 ]. (hindawi.com)
Interestingly, expression of the cell cycle inhibitor p21 (CDKN1A) was regulated by LRH-1 in HCT116 cells. (dundee.ac.uk)
To further confirm, the expression of candidate genes was investigated in exosomes derived from the parental HT-29 colorectal cancer cell line (HT-29-EXOs), and cancer stem cells (CSCs) -enriched spheroids (CSC-EXOs) derived thereof. (researchsquare.com)
Expression is particularly high in the pancreas where it is thought to play a role in the differentiation of pancreatic beta cells. (embl.de)
Alpha-linolenic acid increases cholesterol efflux in macrophage-derived foam cells by decreasing stearoyl CoA desaturase 1 expression: evidence for a farnesoid-X-receptor mechanism of action. (nuthealth.org)
To tackle this issue, we have used cancer cells genetically deficient for the DNA methyltransferase enzymes in conjuction with circRNA expression microarrays. (oncotarget.com)
Data mining for 5′-end CpG island methylation of TUSC3, ATRNL1, POMT1 and SAMD4A in cancer cell lines and primary tumors showed that the epigenetic defect was commonly observed among different tumor types in association with the diminished expression of the corresponding transcript. (oncotarget.com)
The p53 protein is a transcription factor that normally inhibits cell growth and stimulates cell death in response to myriad stressors, including DNA damage (induced by either UV or chemical agents such as hydrogen peroxide), oxidative stress, and deregulated oncogene expression [6-10]. (bioinf.org)
Antisense TGFα approaches showed that the dysregulated TGFα expression in quiescent HCT116 cells is a function of the strong TGFα autocrine loop (not inhibited by blocking antibodies) in these cells. (elsevierpure.com)
This study is the first to report that inhibition of oxaliplatin-induced cell growth may be dependent on p53 and may involve increased expression of cytochrome enzymes (CYP2S1) in CRC cells. (immune-source.com)

These may affect tumour growth and also provide an anti-fatigue effect for cancer patients, in particular for those suffering from lung cancer. (nih.gov)
NT has shown to exert numerous oncogenic effects involved in tumour growth and metastatic spread. (springer.com)
Adiponectin administration suppressed implanted tumour growth, causing larger central necrotic areas. (nuthealth.org)

Herein, we sought to determine if rapamycin, an inhibitor of mTOR, could enhance erlotinib sensitivity for cell lines derived from a variety of tissue types (non-small-cell lung, pancreatic, colon, and breast). (aacrjournals.org)
ASPC is a pancreatic tumor cell range that was proven to maintain imprinting [31C33] previously. (woofahs.com)

PAKs are downstream effectors of the Rho family GTPases, which regulate cell motility and survival. (biomedcentral.com)
Activated PAK1 enhances cell survival and migration via the AKT pathway, and stimulates transformation through the Ras/Raf/ERK/MAPK pathway. (biomedcentral.com)
however, purvalanol triggered autophagy, which functions as a cell survival mechanism at early time-points. (spandidos-publications.com)

One of 3 strains of malignant cells isolated from a male with colonic carcinoma. (xlbiotec.com)
Autocrine transforming growth factor α (TGFα) is an important positive growth effector in maligant cells and plays a significant role in generating the growth factor-independent phenotype associated with malignant progression. (elsevierpure.com)
These inhibitors act on a variety of SIRTs, strengthening the idea that the concomitant inhibition of several SIRTs may contribute to a reduction in malignant growth [ 23 ]. (biomedcentral.com)
Over-production of the growth factor promotes the malignant behavior of tumor cells through enhanced cell growth and CSC self-renewal [26], and loss of imprinting (LOI) is associated with tumor initiation [27, 28]. (woofahs.com)

Body 1 Inhibition of colorectal tumor cell development by oxaliplatin. (immune-source.com)
Imprinting position in tumor cell lines. (woofahs.com)

We find that the DDX5 locus is frequently amplified in breast cancer and that breast cancer-derived cells with amplification of DDX5 are much more sensitive to its depletion than breast cancer cells and a breast epithelial cell line that lacks DDX5 amplification. (aacrjournals.org)

A high-fat diet containing whole walnuts (Juglans regia) reduces tumour size and growth along with plasma insulin-like growth factor 1 in the transgenic adenocarcinoma of the mouse prostate model. (nuthealth.org)

Purvalanol, a novel cyclin-dependent kinase inhibitor, is referred to as a strong apoptotic inducer which causes cell cycle arrest in various cancer cells such as prostate, breast and colon cancer cell lines. (spandidos-publications.com)
Purvalanol is a purine-derived CDKI that binds with a high selectivity and competitively to the ATP binding side of CDK1/2 and leads to G2/M cell cycle arrest ( 4 ). (spandidos-publications.com)
Under this problem, p53-positive cells exhibited arrest in the G1 stage, whereas p53-null cells inserted the S stage and dropped their viability due to the deposition of DNA harm, recommending that MCMBP is certainly a potential focus on for eliminating p53-deficient malignancies. (treatmentforprostatecancer.info)

Cancer stem cells in basic science and in translational oncology: can we translate into clinical application? (woofahs.com)

These cell lines were treated with an extract from E. senticosus that was evaporated and re- constituted in DMSO. (nih.gov)
Recent reports have shown that the sensitivity of non-small-cell lung cancer cell lines to EGFR inhibitors such as erlotinib (Tarceva, OSI Pharmaceuticals) is dependent on inhibition of the phosphatidylinositol 3′-kinase-phosphoinositide-dependent kinase 1-Akt-mTOR pathway. (aacrjournals.org)
Erlotinib could inhibit extracellular signal-regulated kinase, Akt, and S6 only in cell lines that were the most sensitive. (aacrjournals.org)
Therefore, in select cell lines, inhibition of both S6 and Akt was achieved only with the combination of erlotinib and rapamycin. (aacrjournals.org)
Cell lines were treated with 500 nM 5-azacitidine for 72 hours while in log-growth phase, changing the media and drug every 24 hours for AZA treatment. (nih.gov)
Breast, colon, and ovarian cancer cell lines were maintained under ATCC recommended growth conditions. (nih.gov)
The extract also showed a dose dependent growth inhibition of all tested cell lines, IC 50 values against HCT-116, HUVEC, CCD-18CO and HT-29 were 14, 23, 50 and 52 μg mL -1 , respectively. (scialert.net)
These results showed that n-hexane extract of S. koetjape possess both anti-angiogenic and apoptotic properties on colon cancer cell lines making it a good candidate for further studies. (scialert.net)
confirmed an apoptotic death for both cell lines. (bioinf.org)
HCT116 cell lines which differ only within their p53 status. (immune-source.com)

We explored molecular markers shared between TDEs and circulating tumor cells (CTCs) in the blood of cancer patients to identify candidate genes involved in CRC metastasis. (researchsquare.com)
On top of this, 5-FU resistant CRC cells have been shown to exhibit an aggressive phenotype with increased cell invasion and metastasis. (pall.com)

Moreover, inhibitor of PI3K/AKT signaling pathway could inhibit paraptosis-like cell death induced by 125 I seeds radiation. (hindawi.com)

Cells are tumourigenic in nude mice and form colonies on agarose. (xlbiotec.com)

In the present study, our aim was to determine the time-dependent, ER-mediated apoptotic and autophagy induction of purvalanol in HCT 116 colon cancer cells. (spandidos-publications.com)
Moreover, we determined that during purvalanol-mediated ER stress, autophagic machinery was also activated prior to apoptotic cell death finalization. (spandidos-publications.com)
Olomoucine, roscovitine and purvalanol are examples of CDK inhibitors (CDKIs) designed and investigated for their apoptotic potential on cancer cells ( 3 ). (spandidos-publications.com)
Τheir impact on the apoptotic cell death mechanism requires further elucidation. (spandidos-publications.com)
At 50 μg mL -1 , the extract had potently induced apoptotic cell death of HCT-116 colon cancer cell line by inducing caspases 3 and 7 activity. (scialert.net)

Our study suggests an important role for LRH-1 in the growth of CRC cells that retain wild-type p53. (dundee.ac.uk)

This review highlights some of the major findings pertinent to the epidermal growth factor (EGF) receptor and its ligands, particularly the major gut ligand transforming growth factor-alpha, as well as the trefoil peptides. (embl.de)
Betacellulin (BTC) belongs to the epidermal growth factor (EGF) family of peptide ligands that are characterised by a six-cysteine consensus motif that forms three intra-molecular disulfide bonds crucial for binding the ErbB receptor family. (embl.de)
Since 2011, several quinoline compounds have shown Epidermal Growth Factor Receptor (EGFR) inhibition 22 . (nature.com)

Akt also regulates the mammalian target of rapamycin (mTOR)-S6K-S6 pathway to control cell growth in response to growth factors and nutrients. (aacrjournals.org)

We found that 125 I seeds radiation activated PI3K/AKT signaling pathway and could trigger paraptosis-like cell death. (hindawi.com)
Our data suggest that 125 I seeds radiation can induce paraptosis-like cell death via PI3K/AKT signaling pathway. (hindawi.com)

Independent experiments confirmed several target genes, including PLOD2 , HADH , LCOR and REEP1 as non-canonical target genes in various colon cancer cells. (nature.com)
TIF) pone.0151803.s003.TIF (2.3M) GUID:?05DEED38-69F5-444C-AC67-FC01E4C31A93 S4 Fig: More photos of the cells from your same experiments shown in Fig 7. (irjs.info)

Further, in XWLC-05 (Yunnan lung cancer cell line), E. senticosus methanolic extract displayed an inhibitory effect which plateaued with increasing dosage. (nih.gov)
Finally in colon cancer cell line (HCT-116) we observed an initially weak inhibitory effect and then plateau. (nih.gov)

Increased levels of polyamines in cells are considered to be involved in cancer progression. (spandidos-publications.com)
All somatic cells proliferate via a mitotic process determined by progression through the cell cycle. (bioinf.org)
However, the molecular mechanisms by which TGFα confers a growth advantage in progression is poorly understood. (elsevierpure.com)
The cells were analyzed by circulation cytometry for monitor cell cycle progression. (irjs.info)

Marine tests showed an inhibition of growth with the two different algal species (P. tricornutum and D. tertiolecta), an inhibition of bioluminescence in the bacterium A. fischeri, an alteration on the correct early development of the serpulid F. enigmaticus larvae and immobilization of the copepod A. tonsa individuals at both 24 and 48 hours of exposure. (easychair.org)

The cell line has been used in tumorigenicity studies, along with other research that has shown that Cyclin D1 holds large importance for the activity of lithocholic acid hydroxyamide. (wikipedia.org)
BTC was initially described, purified and cloned from a mouse insulinoma cell line. (embl.de)
Description: The HCT116 cell line was established by Brattain et al. (xlbiotec.com)
cell line in which the general population of cells maintain imprinting. (woofahs.com)
In the CSCs derived from this cell line, was also biallelically expressed (right panel). (woofahs.com)
The dichloromethane extract from the bark of Aglaia lanuginose showed cytotoxicity against HL-60 leukaemia cell line (45% inhibition) at 20 µg/ml and was prioritised for further investigation. (afpm.org.my)

CDKs show their action by interacting with cyclins and different CDK-cyclin complexes regulate the cell cycle in the G1, S and G2/M phases ( 1 ). (spandidos-publications.com)

Butyrate is a short-chain fatty acid (SCFA) derived from dietary fiber by bacterial fermentation in the colon and is an important mediator of colonic cell homeostasis. (pall.com)
Butyrate is efficiently absorbed by colonic epithelial cells and provides the primary energy source for the cells of the colon. (pall.com)

The cells have an epithelial morphology and can metastasize in xenograft models. (wikipedia.org)
E , F ) HCT116-Or ( E ) and H716 ( F ) cells were selected to construct the subcutaneous xenograft model of nude mice, so as to observe whether KIF20A silencing would affect the suppression of oxaliplatin on colorectal cancer in vivo . (aging-us.com)

The growing interest in developing methods for personalized medicine, in which tumor cells from individual patients are tested for sensitivity to a panel of drugs 4 , has led to a need for more relevant, time-sensitive studies. (moleculardevices.com)
A new study shows how gut microbiota increases the sensitivity of colorectal cancer cells to chemotherapy. (pall.com)
catenin signaling and cytochromeP450 enzymes (CYP51A1) were correlated to oxaliplatin sensitivity in 21 colorectal cancer cell lines24. (immune-source.com)

We further focused our interest on the molecular mechanisms that underlie 125 I seeds radiation-induced paraptosis on colorectal cancer cells. (hindawi.com)
The goal of this study was to investigate the anti-cancer mechanism of EOPK in HCT116 colorectal cancer cells. (biomedcentral.com)
Here we provide evidence that there may be a direct link between inhibition of intestinal cancer growth and selective inhibition of the COX-2 pathway. (nih.gov)
The critical challenge in cancer treatment is how to effectively kill cancer cells without affecting healthy cells. (hindawi.com)
Transient receptor potential (TRP) channels, which are membranous ion channels that conduct calcium and sodium ions, have been shown to influence cancer cell growth. (dovepress.com)
Given the high frequency of DDX5 amplification in breast cancer, our results highlight DDX5 as a promising candidate for targeted therapy of breast tumors with DDX5 amplification, and indeed we show that DDX5 inhibition sensitizes a subset of breast cancer cells to trastuzumab. (aacrjournals.org)
Tumor-derived EV modulates cellular activities in recipient cells by transferring genetic information from cancer cells. (researchsquare.com)
Objective: To investigate the effects of adiponectin on the growth of colorectal cancer in adiponectin-deficient or wild-type-C57BL/6 mice fed a low-fat or high-fat diet. (nuthealth.org)
This finding suggests that aberrant imprinting may be an intrinsic epigenetic control mechanism that enhances stemness, self-renewal and chemo/radiotherapy resistance in cancer stem cells. (woofahs.com)

Dendrite pruning requires the developmentally regulated degradation of the cell adhesion molecule Neuroglian , and loss of Rab11 causes defects in the developmental degradation of Neuroglian and another target, the ion channel Ppk26 . (sdbonline.org)

Methods for modulating the level of a chemokine in a cell by administering to a cell an effective amount of a depside or an anthocyanin are provided. (justia.com)
Materials and Methods Mammalian cell tradition and harvest HeLa, HEK 293T and A549 cells [13, 14] were cultured in Dulbeccos Modified Eagles Medium (pH7.4) with 10% (v/v) Fetal Bovine Serum at 37C. (irjs.info)

This same sequence confers TGFα promoter responsiveness to exogenous growth factor or autocrine TGFα. (elsevierpure.com)

Anatomy4

Organisms2

Diseases6

Chemicals and Drugs14

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Psychiatry and Psychology1

Phenomena and Processes15

Anthropology, Education, Sociology and Social Phenomena1

Named Groups4

Health Care3

Proliferation29

HT297

Apoptosis11

Intracellular3

Assay6

Vitro6

Knockdown2

Cancers4

Pathways4

Assays1

Regulation5

MRNA5

Endoplasmic reticulum4

Genomic2

Gene8

Therapeutic4

Phosphorylation2

Adherent1

Human13

Expression10

Tumour growth3

Pancreatic2

Survival3

Malignant4

Tumor cell2

Epithelial cell line1

Adenocarcinoma1

Arrest3

Oncology1

Lines10

Metastasis2

Inhibitor1

Colonies1

Apoptotic5

Wild-type p531

Epidermal growth3

Regulates1

Signaling pathway2

Experiments2

Inhibitory effect2

Progression4

Inhibition of Growth1

Line6

Regulate1

Colonic2

Xenograft2

Sensitivity3

Cancer9

Adhesion molecule1

Methods2

Autocrine1