Growth and differentiation of stem cells. Medical search. Frequent questions

Our approach is to first understand how hESC interact with extracellular matrix materials by analyzing candidate adhesive substrate proteins and integrin receptors that mediate attachment, survival, proliferation and differentiation. (ca.gov)
Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
These genes increase the proliferation and differentiation of cells associated with the hair follicle matrix. (medlineplus.gov)

We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
This protocol describes a detailed procedure for resuspending and culturing human stem cell derived neurons that were previously differentiated from neural progenitors in vitro for multiple weeks. (jove.com)
The main aim of this research project was to regenerate AF tissue in vitro using hASCs and serum free chondrogenic medium without supplementation of growth factors. (utwente.nl)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
The effects of iPSCs-imDCs and SN-iPSCs-imDCs on T-cell stimulatory function, and regulatory T (Treg) cell proliferative function in vitro were analyzed by mixed lymphocyte reaction. (newswise.com)
This study focused on exploring sinomenine (SN) to promote differentiation of induced pluripotent stem cells (iPSCs) into imDCs (SN-iPSCs-imDCs), and found SN-iPSCs-imDCs with worse donor-specific T-cell stimulatory function, and higher regulatory T-cell proliferative function in vitro and in vivo to induce high immune tolerance. (newswise.com)
In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
In some cases, an increase in oligodendrocyte differentiation alongside neurons is further highlighted for functional improvements where the biomaterial was then tested for increased myelination both in vitro and in vivo. (karger.com)
Here, we show that TBX3 is dynamically expressed during specification of the mesendoderm lineages in differentiating embryonic stem cells (ESCs) in vitro and in developing mouse and Xenopus embryos in vivo. (nih.gov)

Meanwhile, BCDFs stimulate these cells to differentiate to mature Ig-secreting B cells. (wikipedia.org)
Cells in early-stage activation differentiate in response to IL-2 and all B cells proliferate in the presence of IL-2. (wikipedia.org)
Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
Human induced pluripotent stem (iPS) cells can differentiate into somatic cells ( 1 ). (spandidos-publications.com)
MSCs are a population of nonhematopoietic adult stem cells that have the property of self-renewal and can differentiate into multiple lineages [ 8 - 10 ]. (hindawi.com)
Recently, a number of studies have confirmed that ADSCs possess the ability to differentiate into adipocytes, osteoblasts, and chondrocytes [ 16 - 18 ], suggesting that a broader source of stem cells is available for application in tissue engineering. (hindawi.com)
Previous studies have shown that human adipose stem cells (hASCs) are able to differentiate towards AF phenotype when treated with suitable growth factors. (utwente.nl)
Differentiate human pluripotent stem cells (hPSCs) to cells of the endoderm lineage with our specialized cell culture media. (stemcell.com)
To directly differentiate to pancreatic cells, use our pancreatic progenitor kit to generate functional PDX1 + /NKX6.1 + cells that can be matured into insulin-producing beta cells. (stemcell.com)
Embryonic stem cells are intriguing because they can differentiate into any of the specialized tissue cells of the body. (latimes.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
The conventional view of how stem cells differentiate assumes that cells are "instructed" to progress along prescribed signaling pathways. (sciencedaily.com)
It reveals an elegant yet simple system that maintains cells in a stable state, yet enables them to differentiate under the right conditions. (sciencedaily.com)
Instead, it supports the idea that cells differentiate through the collective behavior of multiple genes in a network that ultimately leads to just a few endpoints -- just as a marble on a hilltop can travel a nearly infinite number of downward paths, only to arrive in the same valley. (sciencedaily.com)
But most important, the differences in Sca-1, GATA1 and PU.1 levels across the three cell groups became less pronounced over time, as did the variability in the cells' propensity to differentiate, suggesting that the differences are transient. (sciencedaily.com)
Together, the findings make the case that a slow fluctuation or cycling of gene activity tends to maintain cells in a stable state, while also priming them to differentiate when conditions are right. (sciencedaily.com)
However, despite the common ability of hiPSCs and hESCs to differentiate into all 3 germ layers, their functional equivalence at the single cell level remains to be demonstrated. (jci.org)
The defining characteristics of pluripotent stem cells, which include embryonic stem (ES) cells and induced pluripotent stem (iPS) cells, are straightforward: their ability to self-renew and differentiate into ectoderm, mesoderm, and endoderm germ layers. (genengnews.com)
The most widely accepted method for determining whether a population of cells is truly pluripotent is the teratoma assay, that is, verifying in vivo a cell population's ability to differentiate into each of the three germ layers via teratoma formation in mice. (genengnews.com)
They can differentiate into cells of the mesodermal lineage, such as adipocytes, and osteocytes. (tocris.com)
In other words, during each growth cycle, the McSCs can differentiate into a pigment-producing partially differentiated state and then revert back to an undifferentiated state. (medicalnewstoday.com)
Stem cells in the body are responsible for the regeneration of tissue in the body and can differentiate to form an array of specialized cells. (medicalnewstoday.com)
Specifically, the division of a stem cell can result in the formation of identical daughter stem cells and/or cells that can differentiate to assume different fates. (medicalnewstoday.com)
Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
SHED was able to differentiate into epithelial like cells when cultured in keratinocyte growth medium (KGM) 2 . (bvsalud.org)

Among these, adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cell isolated from adipose tissue, which has the advantages of abundant storage in vivo , easy acquisition, and expansion [ 13 - 15 ]. (hindawi.com)
This review provides a brief history of mesenchymal stem cell (MSCs) and provides an updated view of the many stem/progenitor cell populations contributing to fracture repair. (researchgate.net)

Gene therapy and monoclonal antibodies didn't arrive with even a fraction of the hype surrounding embryonic stem cell research. (latimes.com)
The T-box transcription factor TBX3 has been implicated in regulating embryonic stem cell self-renewal and cardiogenesis. (nih.gov)

Mesenchymal stem/stromal cells (MSCs) are adult stem cells with the capability of self-renewal and multilineage differentiation into skeletal and/or mesodermal tissues, including bone, fat, cartilage, and muscle tissues. (aibg.it)
The diverse shapes of plants are generated through localised cell division and differential cell growth in meristematic tissues. (jic.ac.uk)
The method currently used to produce stem cell-derived tissues has a very limited throughput. (labmanager.com)
Particularly, human embryonic stem cells (hES cells), which can give rise to all tissues of an organism (Figure A), are used to generate mature cells of the heart (Figure B) allowing us to study radiation/microgravity-induced cardiovascular alterations, which may occur during long-term space missions. (gsi.de)
Stem cells have potential as a source of cells and tissues for research and treatment of disease. (tocris.com)
Cells Tissues Organs (2016) 202 (1-2): 85-101. (karger.com)
This protein is present in many types of cells and tissues, where it is primarily found at junctions that connect neighboring cells (adherens junctions). (medlineplus.gov)
In adult tissues, this pathway plays a role in the maintenance and renewal of stem cells, which are cells that help repair tissue damage and can give rise to other types of cells. (medlineplus.gov)
Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. (bvsalud.org)
Stem cells from human exfoliated deciduous teeth (SHED) are multipotent stem cells derived from the pulp tissues of extracted deciduous teeth 1 . (bvsalud.org)
Notch signaling pathway is also involved in the regulation of epithelial cell differentiation in various tissues 5-6 . (bvsalud.org)
Normal proliferative tissues contain multipotent stem cells (SCs) that drive tissue turnover. (medscape.com)
According to the cancer stem cell (CSC) concept, the cellular hierarchy in normal tissues is preserved in a number of hematological and solid malignancies. (medscape.com)
Tumors, like the tissues from which they originate, contain a unique SC population that is capable of self-renewal and of sustaining tumor growth indefinitely. (medscape.com)

In this study, we verified that exosomes derived from osteogenically differentiated ADSCs can promote osteogenic differentiation of ADSCs. (hindawi.com)
Moreover, we found that these differentially expressed exosomal miRNAs connect osteogenic differentiation to processes such as axon guidance, MAPK signaling, and Wnt signaling. (hindawi.com)
This study confirms that alterations in the expression of exosomal miRNAs can promote osteogenic differentiation of ADSCs, which also provides the foundation for further research on the regulatory functions of exosomal miRNAs in the context of ADSC osteogenesis. (hindawi.com)
The results showed that MSC did not alter their osteogenic differentiation capacity, surface marker profile, and the expression profiles of MSC during expansion. (biomedcentral.com)
Microarray analysis of MSC during osteogenic differentiation identified three candidate genes for further examination and functional analysis: ID4, CRYAB, and SORT1. (biomedcentral.com)
Finally we analyzed the gene expression profiles of MSC during osteogenic differentiation. (biomedcentral.com)
After differentiation, BMP4 and FGF2 treated cells generally produce higher amounts of osteogenic and chondrogenic differentiation than untreated stem cells. (wikidoc.org)

Not all currently known BCGFs and BCDFs affect all B cell lineages and stages of the cell cycle in similar ways. (wikipedia.org)
It is not clear if BCGF I acts on memory B cells specifically, but it appears to induce growth in the continuous presence of anti-Ig in all other lineages. (wikipedia.org)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
The findings also help explain why the process of differentiating stem cells into specific lineages in the laboratory has been highly inefficient. (sciencedaily.com)
What makes characterization challenging is that heterogeneity within a given stem cell population can cause variability in the differentiation into important downstream lineages, such as cardiomyocytes and hepatocytes. (genengnews.com)
[10] FGF2, in conjunction with BMP4 , promote differentiation of stem cells to mesodermal lineages. (wikidoc.org)
SHED has the ability to be differentiated to specific cell lineages such as odontoblasts and osteoblasts as well as epithelial like cells. (bvsalud.org)

(C) Aggrecan-to-type II collagen gene-expression ratio in BM and AD-MSCs after culture in type I collagen hydrogels for 14 days with either no growth factor, TGF-β, GDF5, or GDF6. (biomedcentral.com)
Approximately 50 years have passed since MSC-like cells were first discovered, and many studies have been carried out to outline the characteristics of MSCs and to investigate their potential therapeutic implications in regenerative medicine and immunomodulation. (aibg.it)
We are particularly interested in studies illustrating intrinsic/extrinsic cellular and molecular factors regulating stemness, growth, and differentiation of tissue-specific MSCs as well as their potential implications for tissue engineering and regenerative medicine. (aibg.it)
Over the last decades, mesenchymal stem cells (MSCs) have attracted extensive attention in the field of bone regeneration [ 4 - 7 ]. (hindawi.com)
With the in-depth researches of MSCs, an increasing number of studies have indicated that the therapeutic effects of MSCs can be attributed not only to their differentiation capacity but also to their paracrine action [ 21 ]. (hindawi.com)
Mesenchymal Stem Cells (MSCs) are a heterogeneous subset of stromal stem cells that originate from the mesoderm layer of the fetus. (tocris.com)
Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
The study also showed that the adult OCRs are distinct from mesenchymal stem cells (MSCs), which play a role in bone generation during development and adulthood. (sciencedaily.com)
Researchers presumed that MSCs were the origin of all bone, cartilage, and fat, but recent studies have shown that these cells do not generate young bone and cartilage. (sciencedaily.com)
The CUMC study suggests that OCR stem cells actually fill this function and that both OCR stems cells and MSCs contribute to bone maintenance and repair in adults. (sciencedaily.com)

In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. (tocris.com)
This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. (tocris.com)
In one of the locations-called the bulge- these McSCs undergo self-renewal to maintain a population of immature stem cells. (medicalnewstoday.com)
Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
SCs are not only capable of maintaining themselves (self-renewal) but also give rise to a proliferative 'transit amplifying' compartment and ultimately to postmitotic differentiated cells with specialized functions. (medscape.com)
By contrast, transit amplifying cells and differentiated cells are incapable of self-renewal and tissue regeneration. (medscape.com)

In addition, we examined whether these substrates will support differentiation of retinal pigmented epithelial cells, an important, clinically relevant cell type that is currently in clinical trials for eye disease. (ca.gov)
We earlier reported enrichment of CSC-like side population (SP) with increasing resistance towards Cisplatin and Paclitaxel either alone or in combination in epithelial ovarian cancer (EOC) cells. (techscience.com)
Since the Notch signaling pathway molecules play an important role in differentiation of epithelial cells, it is important to identify the presence of notch signaling molecules in SHED during the process of cell differentiation. (bvsalud.org)
Basal cell carcinoma (BCC) is a nonmelanocytic skin cancer (ie, an epithelial tumor) that arises from basal cells (ie, small, round cells found in the lower layer of the epidermis). (medscape.com)

BCGF I induces "resting" cells to become susceptible to stimulation by ligands. (wikipedia.org)
Induces differentiation in late-stage activated cells. (wikipedia.org)
IFN-alpha, beta 2, and gamma (interferons alpha, beta 2, and gamma) - IFN-gamma in combination with IL-2 also induces early-stage differentiation. (wikipedia.org)
Intriguingly RUNX1 inhibition not only induces CSC differentiation but also downregulates anti-apoptotic protein BCL2 in both SP and NSP cells and potentiates cytotoxic effects of Cisplatin-Paclitaxel in chemoresistant EOC cells. (techscience.com)
This involves placing stem cell clusters into a special medium that induces the spontaneous formation of undeveloped neurons, followed by their differentiation into retinal cells. (labmanager.com)
In the presence of testosterone , FSH stimulates the Sertoli cells and induces spermatogenesis. (msdmanuals.com)

Aizawa, Y., N.D. Leipzig, T. Zahira, M.S. Shoichet (2008) The effect of immobilized platelet derived growth factor AA on neural stem/progenitor cell differentiation on cell-adhesive hydrogels. (karger.com)

Most of these paracrine secretions include soluble factors and exosomes, which regulate the repair and regeneration processes at sites of damage by affecting cell proliferation, migration, and differentiation [ 22 , 23 ]. (hindawi.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The expression of Prx1 has been used as a marker to define the skeletal stem cells (SSCs) populations found within the bone marrow and periosteum that contribute to bone regeneration. (researchgate.net)
Our study suggests that melanocyte stem cells are mobile but can start the regeneration of hair melanocytes only when they are present in a specific area within the hair follicle (hair germ compartment). (medicalnewstoday.com)
however, stem cell injection alone has been unsuccessful in many areas of neural regeneration. (karger.com)
and stem cell regeneration. (lu.se)

How stem cells grow and divide, and how developmental programs and environmental cues are integrated into stem cell maintenance and differentiation remain largely unclear. (jic.ac.uk)
Pluripotent stem cells have great potential for use in regenerative medicine and are an important tool for understanding developmental and disease mechanisms. (genengnews.com)
Additionally, we were able to reconstruct the three developmental phases during osteoblast differentiation: proliferation, matrix maturation, and mineralization, and illustrate the activation of the SMAD signaling pathways by TGF-β2 and BMPs. (biomedcentral.com)
Knowledge on the expression analysis of Notch signaling pathway molecules in SHED cultured in KGM could highlight its involvement in controlling the biological activity of these stem cells, particularly during odontogenesis and other developmental process. (bvsalud.org)
The research at Lund Stem Cell Center is diverse and covers several aspects of developmental and stem cell biology, including but not limited to restorative neurology, hematopoiesis, endocrine cell differentiation, lung bioengineering and bone formation processes. (lu.se)

Weibing' s research focuses on plant stem cell dynamics, aiming to understand the molecular cellular mechanisms underlying stem cell maintenance and differentiation. (jic.ac.uk)

AJOU Open Repository: Monolayer Graphene-Directed Growth and Neuronal Differentiation of Mesenchymal Stem Cells. (ajou.ac.kr)
The development of an efficient platform for the growth and neuronal differentiation of stem cells is crucial for autologous cell therapy and tissue engineering to treat various neuronal disorders and neurodegenerative diseases. (ajou.ac.kr)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)

Most factors act in many points throughout the B cell lifecycle, activation, growth, differentiation, and maturation, making this a complex pathway for study. (wikipedia.org)
However, these cells have limitations, such as rapid maturation and a short lifespan in vivo . (newswise.com)
In previous studies, induced pluripotent stem cells (iPSCs) differentiated into imDCs, and sinomenine (SN) was used to inhibit the maturation of imDCs. (newswise.com)

Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
Thus, we conclude that monolayer graphene is capable of regulating the growth and neural differentiation of hMSCs, which would provide new insight and strategy not only for autologous stem cell therapy but for tissue engineering and regenerative medicine based on graphene scaffolds. (ajou.ac.kr)
This may allow scientists to culture healthy tissue cells in the lab to replace damaged ones in the human body. (latimes.com)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
By semi-automating tissue differentiation, researchers from the Moscow Institute of Physics and Technology (MIPT) and Harvard have made the process nearly four times faster, without compromising on quality. (labmanager.com)
The researchers compared the quality of the robot- and human-grown cells by producing several thousand retinal tissue samples for automatic processing and the same amount for manual handling. (labmanager.com)
Since this protein is only produced in developing retinal cells, the high fluorescence intensity points to the parts of the sample with the right tissue. (labmanager.com)
The automation algorithm was able to optimize cell production by simultaneously testing many systems-without any adverse effect on tissue quality. (labmanager.com)
We also developed a tool for automatic retina identification and organoid classification, which we showed in action, optimizing cell specialization conditions and monitoring tissue quality. (labmanager.com)
One of our goals in this research has been to scale up cell differentiation to enable a high-throughput tissue production for drug tests and cell transplantation experiments. (labmanager.com)
For this purpose, we use human stem cells that play a pivotal role in tissue development, maintenance and function. (gsi.de)
In such organoids, we can also mimic brain tumor initiation and growth and examine the interaction between normal tissue and tumor tissue upon treatment with ionizing radiation alone or in combination with other treatment modalities such as chemotherapies. (gsi.de)
Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)
One great advantage of MSC is that these cells may be directly obtained from individual patients, thereby eliminating the complications associated with immune rejection of allogenic tissue and infectious diseases. (biomedcentral.com)
The researchers believe that OCR stem cells will be found in human bone tissue, as mice and humans have similar bone biology. (sciencedaily.com)
The researchers also suspect that OCR cells may play a role in soft tissue cancers. (sciencedaily.com)
Like other FGF family members, basic fibroblast growth factor possess broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development , cell growth , morphogenesis , tissue repair , tumor growth and invasion. (wikidoc.org)
Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)
of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)
Normal multipotent tissue stem cells (SCs) are the driving force behind tissue turnover and repair. (medscape.com)
Use of human fetal tissue raises several ethical issues, but are there alternative cell sources that can substitute effectively? (lu.se)
Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)

The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)

Relative gene expression was normalized to mean housekeeping-gene expression and cells without growth-factor stimulation and plotted on a log scale. (biomedcentral.com)

The protein-protein interaction network analysis of 397 upregulated genes in RUNX1-high samples of TCGA data show significant enrichment of pathways known to negatively regulate CSC differentiation. (techscience.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
A new study suggests that the conventional view, which assumes that cells are "instructed" to progress along prescribed signaling pathways, is too simplistic. (sciencedaily.com)
The growth and metastatic properties of BCSCs are regulated by different pathways, which are only partially known. (wjgnet.com)
Using novel stem cell, reprogramming and genome editing technologies we are studying how genetic pathways involved in aging and neurodegenerative disease affect formation and function of neural cells. (lu.se)
Proteomics is emerging as a powerful tool to identify the signaling complexes and pathways that control multi- and pluri-potency and SC differentiation. (medscape.com)

Differentiation factors control cell fate and can sometimes cause matured cells to change lineage. (wikipedia.org)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)

[11] However, a low concentration of bFGF (10 ng/mL) may exert an inhibitory effect on osteoblast differentiation . (wikidoc.org)

These data suggest that the interaction of Thy-1 with beta integrins mediates mEV uptake by lung fibroblasts, which blocks myofibroblastic differentiation, and that mEVs are enriched for miRs that target profibrotic genes up-regulated in IPF fibroblasts. (nih.gov)
We suppose that graphene regulates the interactions at cell-substrate or cell-cell interfaces, consequently promoting the neurogenesis of hMSCs as well as the outgrowth of neurites, which was evidenced by the graphene-induced upregulation of early neurogenesis-related genes. (ajou.ac.kr)
In this study, we show that increased expression of Runt related transcription factor 1 (RUNX1) maintains undifferentiated state of CSC-like SP cells through upregulation of inhibitors of DNA binding/differentiation genes (ID1 and ID3) in late cisplatin-paclitaxel resistant cells. (techscience.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Again, they found tremendous variability within the apparently uniform cell population: more than 3,900 genes were differentially expressed (turned "on" or "off") between the low- and high-Sca-1 cells. (sciencedaily.com)
Here, we used single cell analysis to resolve the gene expression profiles of 362 hiPSCs and hESCs for an array of 42 genes that characterize the pluripotent and differentiated states. (jci.org)

Researchers developed the ATRIVIEW® platform to screen for molecules that promote differentiation neural stem cells (NSCs). (news-medical.net)

This protocol facilitates the use of human pluripotent stem cell-derived neurons for high content screening applications. (jove.com)
Human pluripotent stem cell-derived neurons are increasingly relevant in the areas of basic research, drug development, and degenerative medicine. (jove.com)
Advertorial: Pluripotent Stem Cell Verification: Is There an Alternative to Teratoma Assays? (genengnews.com)
The GloLIVE™ Human Pluripotent Stem Cell Live Cell Imaging Kit and individual GloLIVE antibodies available from R&D Systems allow you to verify the pluripotency status of your stem cells by monitoring the expression of both positive and negative markers ( Figure 1 ). (genengnews.com)
The Scientist brings together a panel of experts to discuss challenges and strategies for optimizing your pluripotent stem cell culture. (the-scientist.com)

Mesenchymal stem/stromal cells (MSC) are the ideal source to study fat formation as they are the progenitors of adipocytes. (mdpi.com)
Asmani, M.N., J. Ai, G. Amoabediny, A. Noroozi, M. Azami, S. Ebrahimi-Barough, M. Navaei-Nigjeh, A. Ai, M. Jafarabadi (2013) Three-dimensional culture of differentiated endometrial stromal cells to oligodendrocyte progenitor cells (OPCs) in fibrin hydrogel. (karger.com)

Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. (nature.com)
Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)
Systems Biology Approach to Study Heterogeneity and Cell Communication Networks in the Tumour Microenvironment. (routledge.com)
Led by Sui Huang, MD, PhD, a Visiting Associate Professor in the Children's Hospital Boston Vascular Biology Program (now also on the faculty of the University of Calgary), and Hannah Chang, an MD/PhD student in Children's Vascular Biology Program, the researchers examined how blood stem cells "decide" to become white blood cell progenitors or red blood cell progenitors. (sciencedaily.com)
We also have a strong interest in basic stem cell biology with a clear focus on neural stem cells and neurogenesis in the adult and aging brain. (lu.se)

We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)

First, we characterized the cell surface receptors for matrix proteins on stem cells and identified specific proteins that will support adhesion and growth of the cells. (ca.gov)
Second, using an interdisciplinary approach, we developed a novel method to screen peptides from these proteins for their ability to support adhesion and proliferation of the stem cells. (ca.gov)
Based on our characterization of cell surface receptors for matrix proteins on stem cells completed in the first year, we went on to test specific, purified proteins that will support adhesion and growth of the cells. (ca.gov)
b Cells were collected and heated in a narrow temperature range, with one sample incubated at 37 °C for expression measurements, protein aggregates were eliminated by ultracentrifugation and the soluble proteins were digested. (nature.com)
IL-6 (BSF-2) - cytokine that acts exclusively as a B cell differentiation factor, stimulating increase in levels of Ig, J-chain mRNA, and proteins. (wikipedia.org)
By transporting "cargos" such as proteins, RNAs, DNAs, and lipids [ 26 ], exosomes regulate the eventual fate of recipient cells. (hindawi.com)
Huang and Chang then looked at the proteins GATA1 and PU.1, transcription factors that normally favor differentiation into red and white blood cells, respectively. (sciencedaily.com)
While laminin and fibronectin are widely used ECM proteins, the one that your cell needs should be determined by test experiments. (sigmaaldrich.com)
[3] [5] FGF2 binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins which themselves constitute a family of closely related molecules. (wikidoc.org)
It has been demonstrated to induce gremlin expression which in turn is known to inhibit the induction of differentiation by bone morphogenetic proteins . (wikidoc.org)
Certain proteins in this pathway attach (bind) to beta-catenin, which triggers a multistep process that allows the protein to move into the cell nucleus. (medlineplus.gov)
Cancer stem cell-associated proteins revealed by proteomics. (medscape.com)

The role of BCSCs in cancer formation, growth, invasiveness, therapy resistance and tumor recurrence is becoming increasingly clear. (wjgnet.com)
Cancer stem cells are thought to be responsible for tumor initiation, dissemination and treatment failure. (jcancer.org)
Mutations in the CTNNB1 gene can cause a type of aggressive but noncancerous (benign) growth called a desmoid tumor. (medlineplus.gov)
The CTNNB1 gene mutations that cause desmoid tumors are somatic, which means they are acquired during a person's lifetime and are present only in tumor cells. (medlineplus.gov)
These mutations are somatic and occur only in kidney cells that give rise to the tumor. (medlineplus.gov)
This active beta-catenin protein promotes Wnt signaling longer than normal, which leads to the unchecked proliferation of kidney cells and tumor development. (medlineplus.gov)
The cancer stem cell theory holds that tumors also contain stem-like cells that drive tumor growth and metastasis formation. (medscape.com)
The CSC is defined as a cancer cell with self-renewing capacity that can regenerate the original tumor in all its differentiated heterogeneity. (medscape.com)
[ 2 ] In addition, some tumor types do not appear to adhere to the CSC concept at all [ 3 , 4 ] or may have multiple independent tumor-initiating cell populations. (medscape.com)
ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)

Qualitative Dynamical Modelling of T-Helper Cell Differentiation and Reprogramming. (routledge.com)
Our Stem Cells review gives an overview of the use of small molecules in the control of stem cell growth & differentiation and somatic cell reprogramming. (tocris.com)

Protocols for hepatocyte differentiation from iPS cells have been studied. (spandidos-publications.com)
Developed in-house, this poster summarizes how small molecules have been used in protocols across the stem cell workflow. (tocris.com)
However, technical challenges persist in the standardization of culture growth conditions and differentiation protocols. (the-scientist.com)
Starting with cell source, choice of donor and recipient, as well as isolation methods, we will then discuss existing expansion protocols (two-/three-dimensional cultivation, basal medium, medium supplements, static/dynamic conditions, and hypoxic/normoxic conditions) and influence of these strategies on the cell functionality after implantation. (frontiersin.org)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)

The findings, published in the May 22 issue of Nature, give a glimpse into how that collective behavior works, and show that cell populations maintain a built-in variability that nature can harness for change under the right conditions. (sciencedaily.com)
They began by examining populations of seemingly identical blood stem cells, and found that a cell marker of "stemness," a protein called Sca-1, was actually present in highly variable amounts from cell to cell -- in fact, they found a 1,000-fold range. (sciencedaily.com)
Moreover, single cell heterogeneity amongst stem cell populations may underlie important cell fate decisions. (jci.org)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)

ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)

We proposed to develop novel, well-defined, synthetic extracellular matrices that support survival and proliferation of human embryonic stem cells. (ca.gov)
Finally, we identified a cyclic RGD peptide that supports growth of human embryonic stem cells. (ca.gov)
There is a critical need for new technologies to facilitate growth and differentiation of human embryonic stem cells (hESC) using clinically acceptable, animal-free reagents. (ca.gov)
We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. (nature.com)
Interferon-alpha can either enhance or suppress differentiation by controlling responsiveness of human peripheral blood B cells to B-cell helper factors, depending on certain environment and context-specific conditions, as its signaling is likely mediated by other cell types. (wikipedia.org)
In addition to human iPSCs, it can be used to replate culture of primary neurons or to resuspend them for fact sorting or single cell sequencing. (jove.com)
In this study, we describe the use of highly uniform graphene platforms that provide unique environments where unusual three-dimensional spheroids of human mesenchymal stem cells (hMSCs) are formed, which is advantageous for the differentiation of hMSCs into neurons. (ajou.ac.kr)
Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
Human embryonic stem cells also exhibit the Warburg effect ( 15 ). (spandidos-publications.com)
Human annulus fibrosus cell (hAFCs) cultured in commercial AF growth medium were used as positive control. (utwente.nl)
The molecular study of fat cell development in the human body is essential for our understanding of obesity and related diseases. (mdpi.com)
A) Colonies of human embryonic stem cells, bright field microscopy, scale bar: 100µm. (gsi.de)
D) Karyotype of a human cell after heavy ion exposure revealed by mFISH technique. (gsi.de)
Human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) are promising candidate cell sources for regenerative medicine. (jci.org)
The kit contains a set of azide-free, fluorochrome-conjugated GloLIVE antibodies that recognize two positive markers and one negative marker of human pluripotent stem cells. (genengnews.com)
Pluripotency of live human stem cell colonies was verified using a NorthernLights™ (NL) 493-conjugated Mouse Anti-Human SSEA-4 Monoclonal Antibody (green) and a NL557-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody (red). (genengnews.com)
Can human chorionic gonadotropin supplements enhance penile growth? (news-medical.net)
To address this question we first developed human oligonucleotide microarrays with 30.000 elements and then performed large-scale expression profiling of long-term expanded MSC and MSC during differentiation into osteoblasts. (biomedcentral.com)
In this study we asked if human MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)
Therefore, our aims are to develop cellular models of human neural aging and degeneration, to investigate underlying mechanisms responsible for dysfunction in neural and neural stem cells in the aging and diseased brain and to dissect the regulation of normal neurogenesis. (lu.se)
Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). (bvsalud.org)
Stem cells from human exfoliated deciduous teeth (SHED) (ALLCells, Alameda, CA, USA) were employed in the current study. (bvsalud.org)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)
5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
Here we describe how this system can be used for human embry- onic stem cells. (lu.se)

IL-5 - cytokine that acts like IL-6, except it can also induce proliferation in B cells, and its effect on differentiation is partially inhibited by IL-4. (wikipedia.org)
IL-5 cannot induce differentiation in cells activated by anti-Ig. (wikipedia.org)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)

Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
Numbers matter: The automation empowered us to produce trillions of retinal neurons for transplantation and we are excited to see the translation of our approach into routine cell manufacture," commented Petr Baranov from the Schepens Eye Research Institute of Massachusetts Eye and Ear. (labmanager.com)
We conducted a cross-sectional study of -herpes- in the host, seldom causing disease unless the immune sys- viruses in febrile pediatric oncology patients (n = 30), with tem is weakened, as occurs after treatment for solid-organ a reference group of febrile pediatric solid-organ transplant and stem cell transplantation. (cdc.gov)
This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)
We still do not know what specific factors contribute to the success in transplantation i.e. what cells are responsible for motor recovery? (lu.se)
The aim of this thesis was to understand how particular factors such as neuronal content, placement and cell source, affect functional outcome after transplantation into the rodent brain. (lu.se)
The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)

We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
Using this marker, the researchers found that OCR cells self-renew and generate key bone and cartilage cells, including osteoblasts and chondrocytes. (sciencedaily.com)
Oct. 8, 2021 Researchers have identified a subpopulation of mesenchymal stem cells in the bone marrow that express the marker CD73. (sciencedaily.com)
RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). (bvsalud.org)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
Anaplastic large cell lymphomas (ALCLs) are distinguished from other lymphomas by their anaplastic cytology and constant membrane expression of the CD30 antigen (an activation marker for B or T cells). (medscape.com)

Blood stem cells with low levels of Sca-1 differentiated into red blood cell progenitors seven times more often than cells high in Sca-1 when exposed to erythropoietin, a growth factor that promotes red blood cell production. (sciencedaily.com)
This kit, used in combination with the analysis of colony morphology, promotes the selection of high-quality, undifferentiated stem cell colonies, thereby reducing wasted time. (genengnews.com)
The Wnt signaling pathway promotes the growth and division (proliferation) of cells and helps determine the specialized functions a cell will have (differentiation). (medlineplus.gov)
Excess beta-catenin promotes the uncontrolled proliferation of cells, allowing the formation of desmoid tumors. (medlineplus.gov)

These antibodies are added to live, unfixed cells in a single-step assay that takes only 30 minutes and has no adverse effects on stemness or proliferation. (genengnews.com)

This is an important, unmet need in the field and development of such a substrate would aid in moving stem cell therapies to the clinic. (ca.gov)
hES cells also serve as a basis for cerebral organoid models (Figure C) to better understand e.g. the mechanisms of cognitive impairment and radiation necrosis as severe side effects of radiation therapies. (gsi.de)
However, for cell therapies MSC have to be expanded and/or manipulated to obtain a sufficient amount of cells that can be subsequently used for treatment. (biomedcentral.com)
Therefore, researchers have begun exploring combined therapies with biomaterials that promote cell growth and differentiation while localizing cells in the injured area. (karger.com)
Our findings raise the possibility that drugs or other therapies can be developed to stimulate the production of OCR stem cells and improve the body's ability to repair bone injury--a process that declines significantly in old age," says Timothy C. Wang, MD, the Dorothy L. and Daniel H. Silberberg Professor of Medicine at CUMC, who initiated this research. (sciencedaily.com)
Since opening its doors nearly 20 years ago, Lund Stem Cell Center has remained committed to excellence in stem cell research and training the next generation of scientists in order to make stem cell therapies a reality of tomorrow. (lu.se)

Bone marrow-derived mesenchymal stem cells (MSC) have been promoted for multiple therapeutic applications. (nih.gov)
A stem cell capable of regenerating both bone and cartilage has been identified in bone marrow of mice. (sciencedaily.com)
Jan. 29, 2020 Scientists used molecular motors to manipulate the protein matrix on which bone marrow-derived mesenchymal stem cells are grown. (sciencedaily.com)

Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. (tocris.com)
Our guide highlights the use of small molecules in stem cell research and cell therapy and lists relevant products. (tocris.com)
The aim of this study was to detect the expression of molecules associated with Notch signallng pathway in SHED cultured in specific differentiation medium, namely, KGM. (bvsalud.org)

From 2014 to 2020, Weibing had been working as a Postdoctoral Research Associate at the Sainsbury Laboratory Cambridge University, where he studied the molecular cellular mechanisms of stem cell dynamics. (jic.ac.uk)

fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)

While these approaches target different diseases and employ different mechanisms and methodologies, one thing is universally true: Their development requires massive amounts of retinal cells for research purposes. (labmanager.com)
We implemented automated liquid change during retinal differentiation and showed it had no negative effect on cell specialization," commented Evgenii Kegeles of the Genome Engineering Lab at MIPT. (labmanager.com)

Dr. Hans Clevers describes how his lab discovered Lgr5 stem cells in the intestine that lead to the culture of mini-guts, or organoids, and how the culture system has evolved. (stemcell.com)
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids. (tocris.com)

BCGFs specifically mediate the growth and division of B cells, or, in other words, the progression of B cells through their life cycle (cell cycle stages G1, S, G2). (wikipedia.org)

This enigmatic subpopulation of cancer cells has therefore emerged as promising therapeutic candidate. (techscience.com)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
The work also suggests a much more efficient way of differentiating stem cells for therapeutic purposes. (sciencedaily.com)
With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)

The results of these analyses revealed that the differentially expressed exosomal miRNAs participate in multiple biological processes, such as gene expression, synthesis of biomolecules, cell development, differentiation, and signal transduction, among others. (hindawi.com)

Cell-fate decisions and pluripotency are dependent on networks of key transcriptional regulators. (nih.gov)
The Notch signaling pathway provides important intercellular signaling mechanisms essential for cell fate specification and it regulates differentiation and proliferation of stem or progenitor cells by para-inducing effects 3-4 . (bvsalud.org)

The stem cells in the hair follicle generate the cells to help regenerate hair follicle cells and facilitate hair growth. (medicalnewstoday.com)
We are now trying to figure out whether we can persuade these cells to specifically regenerate after injury. (sciencedaily.com)

These factors are glycoproteins that act on hematopoietic cells by binding to specific cell surface receptors and stimulating proliferation, differentiation, commitment, and some end cell functional activation. (medscape.com)

See More Use our definitive endoderm (DE) differentiation kit to obtain functional DE cells that are capable of downstream differentiation to hepatic, intestinal, pancreatic and pulmonary cells. (stemcell.com)
Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
To account for this high level of functional variation, verifying the pluripotency of stem cells-from the generation of a new cell line through its expansion for an experiment-is crucial. (genengnews.com)
Purpose of review: The identity and functional roles of stem cell population(s) that contribute to fracture repair remains unclear. (researchgate.net)
Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)

[3] Generally, the 155 aa/18 kDa low molecular weight (LMW) form is considered cytoplasmic and can be secreted from the cell, whereas the high molecular weight (HMW) forms are directed to the cell's nucleus. (wikidoc.org)

The heterogeneous expression of markers, together with their rare frequency (1-10 CTCs in 8 mL of blood) are the main intrinsic factors hampering CTC isolation and limiting in-depth study of these cells in patients with melanoma. (nature.com)

Regulation of melanocyte differentiation and growth. (ox.ac.uk)
Hair follicles contain several different types of cells, including melanocyte stem cells that generate cells that produce the pigment melanin responsible for hair color. (medicalnewstoday.com)
A new study in mice shows that these melanocyte stem cells migrate between two sites in the hair follicle during each cycle of hair growth and shedding- from a site where they produce the pigment for hair color to another where they produce stem cells. (medicalnewstoday.com)
The study found that aging results in a greater portion of these melanocyte stem cells getting stuck at the site where they produce stem cells, which leaves a smaller portion of stem cells to generate melanin-producing cells, resulting in the graying of hair. (medicalnewstoday.com)
The increase in the number of follicle growth cycles with aging is associated with deficits in melanocyte stem cells (McSCs) , the stem cells residing in the follicle that can form hair pigment-producing melanocytes. (medicalnewstoday.com)
Melanocyte stem cells are found in two distinct locations at the base of each hair follicle. (medicalnewstoday.com)
D . , a cell biologist at New York University, said, "[Our] analysis revealed melanocyte stem cells are more dynamic/mobile than previously thought. (medicalnewstoday.com)
We revealed that as melanocyte stem cells move within the hair follicle, stem cells can reversibly alter cell state from immature to mature state, and this reversibility is critical for the proper maintenance of these stem cells. (medicalnewstoday.com)
Dr. Ito also noted, "The study is built upon previous studies showing that maintaining healthy melanocyte stem cells is the key to preserving hair color. (medicalnewstoday.com)
Our study also suggests that melanocyte stem cell localization may be altered during the course of aging. (medicalnewstoday.com)

In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)

He is also interim president of the state's new stem cell agency, the California Institute for Regenerative Research. (latimes.com)
Our results suggest that caution should be exercised before assuming that hiPSCs occupy a pluripotent state equivalent to that of hESCs, particularly when producing differentiated cells for regenerative medicine aims. (jci.org)
They may also serve as an unlimited source of therapeutically active cells for regenerative medi- cine. (lu.se)

Anatomy66

Organisms12

Diseases9

Chemicals and Drugs68

Analytical, Diagnostic and Therapeutic Techniques and Equipment44

Phenomena and Processes57

Disciplines and Occupations4

Technology, Industry, Agriculture1

Information Science3

Proliferation and differentiation3

Vitro10

Differentiate27

Mesenchymal stem2

Embryonic Stem2

Tissues14

Osteogenic7

Lineages7

MSCs12

Self-renewal7

Epithelial4

Induces6

Progenitor cell1

Regeneration6

Developmental5

Maintenance and differentiation1

Neuronal differentiation3

Maturation3

Tissue22

Cardiomyocyte differentiation1

Housekeeping-gene1

Pathways6

Lineage2

Osteoblast differentiation1

Genes6

Promote differentiation1

Pluripotent stem5

Stromal2

Biology5

Neural differentiation1

Proteins12

Tumor10

Reprogramming2

Protocols7

Populations5

Abstract1

Human30

Induce4

Transplantation7

Marker6

Promotes4

Stemness1

Therapies6

Bone marrow3

Molecules4

20201

FACS1

Retinal2

Organoids2

Mediate1

Therapeutic5

Differentially1

Fate2

Regenerate2

Receptors1

Functional5

Molecular1

Heterogeneous1

Melanocyte10

Vascular endothelial cells1

Regenerative3