BrdU [5-Bromo-2'-deoxyuridine]is a well known biomarker for cell cycle and cell proliferation. (eurogentec.com)
deoxyuridine (BrdU) to trace the fate of mitotic cells. (ewha.ac.kr)
On the contrary, the ratio of the BrdU and NeuN double-positive cells did not show any significance, even though it was slightly higher in the knockout group. (ewha.ac.kr)
MIN6 cellular proliferation and viability were measured by 5-bromo-2-deoxyuridine (BrdU) enzyme-linked immunosorbent assay, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and lactate dehydrogenase assays. (ulster.ac.uk)
5-bromo-2'-deoxyuridine (BrdU), a thymidine analog, is widely used to measure de novo DNA synthesis. (bdbiosciences.com)
The "Early Origin Meta-Peaks" subtrack corresponds to early origins of replications that were identified by the genome-wide profiling of 5-bromo-2-deoxyuridine (BrdU). (wustl.edu)
Triphosphate6
In the presence of excess ATP, the enzyme ribonucleotide reductase initiates a chain reaction with UDP, which catalyzes the formation of deoxyuridine diphosphate (dUDP), which is then converted to deoxyuridine triphosphate (dUTP), then deoxyuridine monophosphate (dUMP) via the addition or removal of phosphate groups. (wikipedia.org)
The aim of this work was to combine a set of novel 2'-deoxyuridine-5'-triphosphate (dUTP) derivatives modified with Tyr or Trp aromatic groups with polymerase chain reaction (PCR) and isothermal amplification technique such as recombinase polymerase amplification (RPA). (ac.ru)
The cell viability and apoptosis were determined by using adenosine 5′-triphosphate (ATP) assay and terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL), respectively. (molvis.org)
The apoptotic rate was determined by terminal deoxynucleotide transferase-mediated deoxyuridine triphosphate nick-end labeling analysis. (molecularhydrogenstudies.com)
A deoxyadenosine triphosphate (dATP) analogue for DNA labeling was synthesized with the 1-methylcyclopropene (1MCP) group at the 7-position of 7-deaza-2′-deoxyadenosine and applied for primer extension experiments. (dynamic-biosensors.com)
The real-time kinetic data reveals that this 1MCP-modified dATP analogue is incorporated into DNA much faster than that of the similarly 1MCP-modified deoxyuridine triphosphate (dUTP) analogue. (dynamic-biosensors.com)
DUTP1
However, among nucleotides under study, only 5-aminoallyl-dUTP derivative modified with 4-hydroxyphenylacetic acid revealed good compatibility with the RPA assay, probably due to a small size of the additional functional group and linker flexibility. (ac.ru)
Monophosphate5
Deoxyuridine monophosphate (dUMP), also known as deoxyuridylic acid or deoxyuridylate in its conjugate acid and conjugate base forms, respectively, is a deoxynucleotide. (wikipedia.org)
Deoxyuridine monophosphate (dUMP) is the deoxygenated form of uridine monophosphate (UMP), and is the precursor to deoxythymidine monophosphate (dTMP), a component of DNA nucleotide biosynthesis. (wikipedia.org)
The synthesis of deoxyuridine monophosphate (dUMP) is a multi-step process that begins with uridine monophosphate (UMP), the product of pyrimidine biosynthesis. (wikipedia.org)
The rate of dehalogenation of 5-bromo-2'-deoxyuridine 5'-monophosphate (BrdUMP), a reaction catalyzed by TS that does not require CH(2)H(4)folate as cosubstrate, by mutant TSs was analyzed and showed that only S167A and S167G catalyzed the dehalogenation reaction and values of k(cat)/K(m) for the mutant enzymes were decreased by 10- and 3000-fold, respectively. (rcsb.org)
Thymidylate synthase (TS) catalyzes the reductive methylation of deoxyuridine monophosphate (dUMP) by 5, 10-methylenetetrahydrofolate (CH2H4folate) to form deoxythymidine monophosphate (dTMP) and dihydrofolate (H2folate). (rcsb.org)
Synthesis1
In addition, l -citrulline also inhibited serum-induced DNA synthesis, measured as 5-bromo-2′-deoxyuridine incorporation. (aspetjournals.org)
DUMP1
By replacing the hydroxyl group at the 2' carbon of ribose with a hydrogen, UMP becomes deoxygenated to dUMP. (wikipedia.org)
In contrast, the NO-releasing compound S-nitrosoglutathione (GSNO) promoted S-glutathionylation of a thiol group of GAPDH both in vitro and under cellular conditions. (lookformedical.com)
Hydroxyl group1
These values and those already available for the corresponding DNA homologues allow the evaluation of the effect of the hydroxyl group in position 2' of the sugar moiety, which lowers the PAs of RNA nucleosides by 0.6-1 kcal/mol, and of the methyl group in position 5 of the thymine ring which enhances the basicity of deoxythymidine over deoxyuridine by 0.6 kcal/mol. (unical.it)
Transient1
The triplet-triplet absorption spectrum and the rate constants (the intrinsic decay rate constant of the triplet state, the self-quenching rate constant, and the quenching rate constant of the triplet state by an oxygen molecule) of the thio-2′-deoxyuridine were obtained by transient absorption spectroscopy. (open.ac.uk)
Proliferation1
Colonic epithelial cells proliferation of group IV (DPE 250 mg/kgBW) were significantly lower than III and V groups. (biomedcentral.com)
Functional1
Introduction of 5'-terminal functional groups into synthetic oligonucleotides for selective immobilization. (org.ua)
Thymidylate2
The present study evaluated the prognostic value of the epidermal growth factor receptor (EGFR) mutation status, and excision repair cross-complementation group 1 (ERCC1) and thymidylate synthase (TS) expression following intercalated tyrosine kinase inhibitor (TKI) therapy and platinum- and pemetrexed-based chemotherapies (subsequent second-line treatment) for patients with adenocarcinoma non-small-cell lung cancer (AC-NSCLC). (medsci.org)
Several biomarkers have been used to predict treatment responses, such as the EGFR mutation status for targeted therapy or the excision repair cross-complementation group 1 (ERCC1) and thymidylate synthase (TS) expression for conventional chemotherapy [ 3 - 8 ]. (medsci.org)
Fluorescent1
Once the dye is inside the cell, esterases cleave off the ester group to convert the dye into a fluorescent product and trap it inside the cell. (bdbiosciences.com)
Rats were randomly2
The rats were randomly assigned to one of the four groups: vehicle, Ex, SB623, and SB623 + Ex groups. (biomedcentral.com)
Adult male Sprague-Dawley rats were randomly allocated into four groups: sham, OLT, OLT with HRS (6 ml/kg) pretreatment (HS), OLT with HRS and chloroquine pretreatment (60 mg/kg) group (CQ). (molecularhydrogenstudies.com)
Nucleotide1
It is proposed that the catalytic cysteine is activated by substrate binding by a proton-transfer mechanism in which the phosphate group of the nucleotide neutralizes the charge of Arg 126', facilitating the transfer of a proton from the catalytic cysteine to a His 207-Asp 205 diad via a system of ordered water molecules. (rcsb.org)
Oxygen1
The ϕ Δ value of the new thio-2′-deoxyuridine was found to be substantially higher than all reported values of other thio-2′-deoxyribonucleosides in low oxygen concentrations similar to cancer cell environments. (open.ac.uk)
Cells4
Zaprinast, a phosphodiesterase type V inhibitor, enhanced 5-bromo-2′-deoxyuridine incorporation in serum-stimulated cells. (aspetjournals.org)
Nestin- and Ki67-positive proliferating cells did now show any significant differences between wild and knockout groups. (ewha.ac.kr)
This track shows the locations of the early activating origins of replication in Kc, S2, and BG3 cells that have been identified by the modENCODE Origins of Replication Group. (wustl.edu)
Another group of rats received i.c. injection of EdU-labeled allogeneic penile smooth muscle cells (PSMCs). (stemcellclinicsofamerica.net)
Widely1
S-Nitrosylation of protein thiol groups by nitric oxide (NO) is a widely recognized protein modification. (lookformedical.com)
Cell2
It has been classified histologically into two major groups: small-cell lung cancer and non-small-cell lung cancer (NSCLC). (medsci.org)
The ester group allows the dye to enter the cell. (bdbiosciences.com)
Higher1
However, when excited into the higher excited state with non-resonant two-photon absorption, the ϕ Δ of the thio-2′-deoxyuridine derivative was found to remain sufficiently large. (open.ac.uk)
Introduction of nonradioactive reporter groups into synthetic oligonucleotides and their detection. (org.ua)
Space Group1
S167A, S167T, and S167C crystallized in the I2(1)3 space group and scattered X-rays to either 1.7 A (S167A and S167T) or 2.6 A (S167C). (rcsb.org)
Study1
In this study we show that nitrosonium tetrafluoroborate (BF4NO), a NO+ donor, modified the thiol groups of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by S-nitrosylation and caused enzyme inhibition. (lookformedical.com)
Member1
SRY-related high-mobility-group box 9 (SOX9) is a member of the SOX family of transcription factors. (cancerindex.org)
Levels1
Additionally, ephedrine suppressed epithelial-mesenchymal transition (EMT) process stimulated by BLM treatment, as demonstrated by the reduced α-SMA and vimentin levels together with the increased cytokeratin-8 and E-cadherin levels in BLM + Ephedrine group. (bvsalud.org)