• The method uses sulfated alpha-cyclodextrin in the presence of Mg +2 , which forms complexes with apoB containing lipoproteins, and polyethylene glycol-coupled cholesteryl esterase and cholesterol oxidase for the HDL-cholesterol measurement. (cdc.gov)
  • HDL cholesterol was measured by a direct non-precipitating method using polyethylene glycol-coupled cholesteryl esterase (PEG-modified enzyme) and cholesterol oxidase. (who.int)
  • Fasting and Postprandial Blood glucose measured by glucose oxidase method on RA 1000 fully automated Biochemistry Analyser. (statperson.com)
  • In this study we compared blood glucose levels determined by two commonly used glucometers (One Touch® and Accu-check® ) with those of a standard laboratory method and determined the effect of haematocrit on glucose readings Methods: Blood glucose levels were measured with One Touch® and Accu-Check® glucometers and the glucose oxidase method at the same time in 295 children aged 0 to 15 years over a 6-month period. (bvsalud.org)
  • There was an acceptable level of bias (3.9 mg/dL) between the One Touch® and Accu-check® glucometers, but each had a remarkably large bias compared with the glucose oxidase method. (bvsalud.org)
  • In enzymology, a cholesterol oxidase (EC 1.1.3.6) is an enzyme that catalyzes the chemical reaction cholesterol + O2 ⇌ {\displaystyle \rightleftharpoons } cholest-4-en-3-one + H2O2 Thus, the two substrates of this enzyme are cholesterol and O2, whereas its two products are cholest-4-en-3-one and H2O2. (wikipedia.org)
  • This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. (wikipedia.org)
  • The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. (wikipedia.org)
  • To date it has only been exploited on sterols with a 3β-hydroxy-5-ene or 3β-hydroxy-5α-hydrogen structure, using bacterial cholesterol oxidase enzyme to convert the 3β-hydroxy group to a 3-oxo group for subsequent derivatization with the positively charged Girard hydrazine reagents, or on substrates with a native oxo group. (swan.ac.uk)
  • The study indicated that polyherbal extract at a dose of 200 mg/kg body weight showed significant decline in blood glucose level as compared to individual extract.The elevated level of SGPT, SGOT in the diabetic controlled group reflected the significant alteration of liver function by STZ induction and was found to be equipotent to Glipizide in restoration of the elevated enzyme levels to normal. (annalsofrscb.ro)
  • The enzyme L-gulonolactone oxidase that accomplishes this chemical reaction does not work in these beings. (life-enthusiast.com)
  • ACL is an enzyme upstream of 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA) reductase in the cholesterol biosynthesis pathway. (medscape.com)
  • Psychotropic medication used to treat depression and bipolar disorder such as selective serotonin-reuptake inhibitors (SSRIs).tricyclic antidepressants.monoamine oxidase inhibitors.and lithium. (eurotrans.gr)
  • These data indicate RNA metabolism, proteostasis, monoamine metabolism, and cholesterol synthesis are disrupted in the RTT proteome. (biomedcentral.com)
  • In the first topic, layer-by-layer (LbL) films will be fabricated which contain enzymes such as cholesterol oxidase, monoamine oxidase and lipase, and may be incorporated into phospholipid liposomes, in order to seek synergy among the distinct materials in detecting various analytes. (fapesp.br)
  • Donors with higher UA levels had lower eGFRs after donation, whereas body mass index, hemoglobin A1c, blood pressure, and low-density lipoprotein cholesterol did not have any impact on the eGFR. (researchsquare.com)
  • Alterations in plasma lipid and lipoprotein composition including a tendency toward an increase in total cholesterol (TC) and triglyceride (TG) and decrease in high-density lipoprotein cholesterol (HDL-C) levels suggest that psoriasis may associate with the disorders of lipid metabolism [ 25 , 26 ]. (hindawi.com)
  • Elder age, increased systolic blood pressure, increased MBG and elevated low density lipoprotein cholesterol were independent contributors to plaque formation. (biomedcentral.com)
  • While a reduction in low-density lipoprotein- cholesterol (LDL-C) was observed in many of these studies, it is now considered that statins also mediate pleiotropic antiatherogenic effects that are partially independent of their cholesterol-lowering effects and may contribute to their efficacy in reducing cardiovascular events. (biomedcentral.com)
  • Bempedoic acid is a first-in-class adenosine triphosphate-citrate lyase (ACL) inhibitor that lowers low-density lipoprotein cholesterol (LDL-C) by inhibiting cholesterol synthesis in the liver. (medscape.com)
  • The blood glucose level, liver function enzymes, total protein, lipid, and cholesterol levels were significantly normalized by extract treatment. (hindawi.com)
  • Using fluorescence microscopy, researchers can study the distribution of cholesterol at subcellular resolution, detecting the lipid in the Golgi and at the plasma membrane, but also in the endoplasmic reticulum and mitochondria. (vectorlabs.com)
  • Serum TSH, FT3 and FT4 by CLIA, Serum high sensitive C reactive protein by Immunoturbidimetric assay and Lipid parameters analyzed in Erba EM360 autoanalyzer, Serum TG: GPO Method, HDL and LDL cholesterol by precipitation method, Total cholesterol by cholesterol oxidase - peroxidase method were investigated. (medicinepaper.net)
  • The elevated lipid levels (triglyceride and total cholesterol) were restored to near normal by extract for all the estimated parameters. (annalsofrscb.ro)
  • 1. Lipid profile (consisting of total cholesterol, HDL cholesterol, LDL cholesterol, triglyceride concentrations) measured at baseline and after 4 and 8 weeks. (who.int)
  • Blood samples were collected from the retro orbital plexus of the eye and blood glucose level was estimated by glucose oxidase-peroxidase method. (annalsofrscb.ro)
  • Total cholesterol, HDL-cholesterol and triglyceride concentrations were measured in serum by a Roch/Hitachi Modular P Chemistry Analyzer (Mod P). The cholesterol assay used the cholesterol oxidase/peroxidase (CHOD/POD) method. (who.int)
  • The substrate-binding domain found in some bacterial cholesterol oxidases is composed of an eight-stranded mixed beta-pleated sheet and six alpha-helices. (wikipedia.org)
  • The antioxidant markers, glucolytic, and gluconeogenic enzymes were significantly ameliorated and the elevated level of kidney biomarkers in the diabetic groups was restored. (hindawi.com)
  • 27-Alkyne cholesterol is accepted by cellular enzymes from different biological species (Brevibacterium, yeast, rat, human) and these enzymes include cholesterol oxidases, hydroxylases, and acyl transferases that generate the expected metabolites in in vitro and in vivo assays. (vectorlabs.com)
  • Increased polymorphonuclear leukocyte levels damage surrounding tissue by releasing reactive oxygen species produced via nicotinamide adenine dinucleotide phosphate (NADPH) oxidase/myeloperoxidase and proteolytic enzymes. (hindawi.com)
  • Most enzymes of distal cholesterol biosynthesis are membrane bound, which makes them hard to isolate and also affects their interaction with binding partners when removed from their natural environment. (chromatographyonline.com)
  • The apolipoprotein B containing lipoproteins in the specimen are reacted with a blocking reagent that renders them non-reactive with the enzymatic cholesterol reagent under conditions of the assay. (cdc.gov)
  • Q. You developed a GC-MS‑based whole-cell screening assay for the target identification of distal cholesterol biosynthesis (1). (chromatographyonline.com)
  • An interesting assay published by the group of Miguel Lasuncion used LC-scintillation analysis to study distal cholesterol biosynthesis (5).This assay formed the basis for our developments. (chromatographyonline.com)
  • This helped us to design a simple and robust assay, so we could investigate interactions with distal cholesterol biosynthesis. (chromatographyonline.com)
  • After five weeks, the placebo group with impaired photoperiod showed a decrease in the quality and quantity of sperm and a reduction in testosterone, corticosterone, aldosterone, total antioxidant capacity, xanthine oxidase, and melatonin. (biomedcentral.com)
  • Why is the investigation of distal cholesterol biosynthesis important? (chromatographyonline.com)
  • However, several reports in the past decade, including our own, have underlined the bioactivities of the pathway intermediates and identified distal cholesterol biosynthesis as a possibly druggable pathway, particularly in the context of inflammatory diseases (2,3,4). (chromatographyonline.com)
  • The latter has just recently been published in The Proceedings of the National Academy of Sciences (PNAS) (2) and provides a link between distal cholesterol biosynthesis and inflammation resolution. (chromatographyonline.com)
  • The direct method is used for participants ages 3-5 and for participants with no heparin-manganese HDL-cholesterol values, usually as a result of limited sample volume. (cdc.gov)
  • Researchers are cautioned to interpret trends in HDL cholesterol for NHANES 1999-2008 in view of probable HDL cholesterol method effects. (cdc.gov)
  • Profiles were compared according to band positions by using the Dice coefficient and were clustered by using unweighted pair-group method averages. (cdc.gov)
  • Here we describe an extension of the technology by substituting 3α-hydroxysteroid dehydrogenase (3α-HSD) for cholesterol oxidase, making the method applicable to sterols with a 3α-hydroxy-5β-hydrogen structure. (swan.ac.uk)
  • Apolipoprotein B containing lipoproteins are removed by precipitation with heparin sulfate and MnCl 2 and cholesterol is measured in the HDL-containing supernatant. (cdc.gov)
  • Independent Student's t-test was performed to compare metabolic status values in DC and ND groups. (bvsalud.org)
  • Diabetes mellitus (DM) comprises a group of common metabolic disorders that share the salient feature of hyperglycemia. (bvsalud.org)
  • Accumulation of propionic acid apparently results in an abnormal cytochrome-c oxidase. (medscape.com)
  • Variables independently associated with CHD were female sex, age, systolic blood pressure, 2-hour postprandial glucose, body mass index, waist-to-hip ratio and LDL/HDL cholesterol ratio. (who.int)
  • Significant improvements in body weight, body fat, and waist circumference as well as plasma total cholesterol, LDL cholesterol, blood glucose, C-reactive protein, adiponectin and leptin levels were observed in the IGOB131 group compared with the placebo group. (biomedcentral.com)
  • To address whether saccharide moieties of blood groups A, B and O antigens modulate hemolytic activity of Naja naja atra cardiotoxins (CTXs), the present study was carried out. (hifsignaling.com)
  • Unlike other CTX isotoxins, hemolytic activity of CTX3 toward blood group O cholesterol-depleted red blood cells click here (RBCs) was notably lower than that of blood groups A and B cholesterol-depleted RBCs. (hifsignaling.com)
  • with H-antigen alters its membrane-bound mode, thus reducing its hemolytic activity toward blood group O cholesterol-depleted RBCs. (hifsignaling.com)
  • Aim: The objectives of this study were to evaluate the levels of glucose, cholesterol, protein and albumin in saliva, and to correlate the levels of glucose of the saliva to oral health and blood glucose of diabetic and non-diabetic children. (bvsalud.org)
  • Thus, the objectives of this study were to evaluate the levels of glucose, cholesterol, protein and albumin in saliva, and to correlate the levels of glucose of the saliva to oral health and blood glucose of diabetic and non-diabetic children thereby analyzing whether saliva-based glucose testing can be an effective alternative to blood-based glucose testing in children. (bvsalud.org)
  • But it might help lower cholesterol and control blood sugar. (medlineplus.gov)
  • Alkyne cholesterol represents a versatile, sensitive, and easy-to-use tool for tracking cellular cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy. (vectorlabs.com)
  • 2014). A novel alkyne cholesterol to trace cellular cholesterol metabolism and localization. (vectorlabs.com)
  • Right, typical shovel-shaped synergistic hemolysis reactions caused by L. ivanovii sphingomyelinase in the presence of R. equi cholesterol oxidase compared with the negative reaction given by L. monocytogenes (Lm). (cdc.gov)
  • The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as 'click chemistry', in the presence of a copper-containing catalyst. (vectorlabs.com)
  • In the diabetes group, the serum adiponectin level in patients with renal dysfunction (22.3 (21.5-23.1) ng/mL) was higher than in patients with no complications (12.1 (11.45-12.75) ng/mL) and than in patients with coronary artery disease (11.2 (10.25-12.15) ng/mL) (P = 0.009). (biochemia-medica.com)
  • An alternative hypothesis implicates direct basal ganglia damage due to dysfunction of cytochrome-c oxidase. (medscape.com)
  • However, as found by others, derivatization of the resultant 3-oxo group with a hydrazine reagent does not go to completion in the absence of a conjugating double bond in the sterol structure. (swan.ac.uk)
  • This guideline details procedures for the manufacture and evaluation of human serum pools for cholesterol measurement. (fda.gov)
  • Guidance for 510(K)s on Cholesterol Tests for Clinical Laboratory, Physician's Office Laboratory, and Home Use Guidance for Industry, Issued February 1997. (fda.gov)
  • METHODS: Data from US patients treated with BPaL between 14 October 2019 and 30 April 2022 were compiled and analyzed by the BPaL Implementation Group (BIG), including baseline examination and laboratory, electrocardiographic, and clinical monitoring throughout treatment and follow-up. (cdc.gov)
  • They were divided into three groups as follows: Lean, Nonobese and obese. (statperson.com)
  • Aetna considers up to a combined limit of 26 individual or group visits by any recognized provider per 12-month period as medically necessary for weight reduction counseling in adults who are obese (as defined by BMI greater than or equal to 30 kg/m 2 Footnotes ** ). (aetna.com)
  • The mean serum hs - CRP levels in both the study groups was within the reference range, but it was high and statistically significant in cases than in control. (medicinepaper.net)
  • 10 , 11 Dyslipidemia in ESRD patients is characterized by a dysregulation of the synthesis and activity of HDL, leading to decreased plasma levels of HDL cholesterol (HDL-C). 10 Many epidemiologic studies have documented an inverse relationship between HDL-C levels and the progression of atherosclerosis and increased risk of cardiovascular disease in the general population. (lww.com)
  • Elevated levels of cholesterol were evident in DC in correlation with ND. (bvsalud.org)
  • The patients had their saliva collected and evaluated for glucose, cholesterol, total protein and albumin. (bvsalud.org)
  • Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. (wikipedia.org)
  • In addition, compared with control mice, bilirubin-treated mice had reductions in total cholesterol and leptin and increases in adiponectin. (bmj.com)
  • Standard microbiology references describe Stenotrophomonas maltophilia as oxidase negative and variable with respect to utilization of lactose and sucrose. (hifsignaling.com)
  • Therefore, we performed this prospective study to investigate the relationship between plasma testosterone and adiponectin in a group of adult patients with type 2 diabetes. (biochemia-medica.com)
  • In our study, we restricted only patients and controls with both parents from the same ethnic group were included leaving 1,004 RA patients and 1,382 control excuses against the analysis. (centralparkcarriagesofficial.org)
  • Methods: 32 children with type 1 diabetes mellitus formed the study group (DC) and 32 non-diabetic children formed the control group (ND). (bvsalud.org)
  • Total 90 known cases of type 2 diabetes mellitus were taken from 30 to 60 years age group with no h/o Hypertension, chronic kidney disease, stroke and acute myocardial infarction in past. (statperson.com)
  • The average serum testosterone did not differ between the diabetes and the control group, but the average adiponectin in the diabetes group was lower (14.6 (14.2-15.0) vs . 24.3 (24.05-24.55) ng/mL, P = 0.001). (biochemia-medica.com)
  • The incidence of childhood onset type 1 diabetes is increasing in many countries in the world, at least in the under 15-year-old age group. (bvsalud.org)
  • Mice receiving sericin had parameters similar to the control group. (biomedcentral.com)
  • We previously reported that exercise capacity and skeletal muscle mitochondrial function in diabetic mice were impaired, in association with the activation of NAD(P)H oxidase. (elsevierpure.com)
  • C57BL/6J mice were fed a normal diet (ND) or HFD, then treated or not with sesamin (0.2%) to yield the following four groups: ND, ND+Sesamin, HFD and HFD+Sesamin (n = 10 each). (elsevierpure.com)
  • Superoxide anion and NAD(P)H oxidase activity were significantly increased in HFD mice compared with the ND mice and were ameliorated by sesamin. (elsevierpure.com)
  • To reconcile the discrepancies in the original danger characterizations, we derived a dichotomized variable for the duration of each familiarity using the lower or no airing as the cheap fluoxetine reference group in calculating probable PTSD risks ( Table 2, e.g. late arrival, brusque work duration, and absent of revealing). (centralparkcarriagesofficial.org)
  • The data will be used to monitor the status of hyperlipidemia and the success of the National Cholesterol Education Program. (cdc.gov)
  • Methods Circulating (n = 179) and hepatic expression (n = 95) of ghrelin and LEAP-2 were measured in patients with severe obesity and available liver pathology analysis undergoing Roux-en-Y gastric bypass (RYGB). (unav.edu)
  • DESIGN, SETTING, AND PARTICIPANTS: Two-group noninferiority, pragmatic, cluster-randomized trial conducted in US community hospitals, all of which used mupirocin-CHG for universal decolonization in ICUs at baseline. (cdc.gov)
  • The Cardiovascular Research Group has a wide range of cardiovascular research interests covering many aspects of health and disease. (aru.ac.uk)
  • For people who are of Asian descent and some other ethnic groups, the BMIs that are considered normal and overweight are slightly lower. (msdmanuals.com)
  • Examples include lathosterol side-chain amides targeting lathosterol oxidase, several improved inhibitors of squalene epoxidase, and maybe most importantly, a series of selective and highly potent inhibitors of 24-dehydrocholesterol reductase (DHCR24) inhibitors. (chromatographyonline.com)
  • The participants were randomly allocated to one of three dietary supplementation regimens: coconut milk powder 30 g, grated coconut 30 g or coconut oil 30 ml, or the control group. (who.int)
  • At the same time, in these groups, there was an increase in the level of aromatase, malondialdehyde, cholesterol, and steroidogenic factor-1 (SF-1) expression in the adrenal cortex and an enhancement in histological lesions. (biomedcentral.com)
  • The total cholesterol level in cases (179.34 ±45.58 mg/dl) and control (191.67±31.76 mg/dl) were within the reference range and there was no statistical significance (p = 0.79). (medicinepaper.net)
  • The results of the PHE on treated group were found to restore the significant glycemic level to the near normal level thereby indicating antihyperglycemic activity. (annalsofrscb.ro)
  • Based on the analysis of 16s rDNA sequencing, compared to the saline-treated group, oral administration of spirulina once daily for 24 consecutive days altered the diversity, structure, and composition of colonic microbial community at the genus level. (frontiersin.org)
  • Total 100 patients were divided into 2 groups. (medicinepaper.net)
  • Glutaryl-CoA dehydrogenase deficiency in Spain: evidence of two groups of patients, genetically, and biochemically distinct. (irbbarcelona.org)
  • Between the years 1995 and 2003 Dr. Hammarston's group studied 320 patients with biopsy proven prostate cancer. (life-enthusiast.com)
  • 9%). In group 2, patients showed an improvement in total SF-36 score (4.0 ± 6.1 points), physical function domain of SF 36 (1.8 ± 3.4 points), role physical domain of SF 36 (0.6 ± 1.6 points) and vitality domain of SF 36 (2.7 ± 4.6 points) improved. (isciii.es)
  • Significant differences in biochemical and functional parameters among the three groups were demonstrated, in particular for systolic (p = 0.002) and diastolic pressure (p = 0.02) and eGDR (p = 0.039). (biomedcentral.com)
  • The early onset group was characterized by mental retardation and early death, with the median survival period being 3 years. (medscape.com)
  • In NHANES 1999-2000, HDL-cholesterol was measured using two methods. (cdc.gov)