Loading...
  • subunit
  • The pathogenic mutation examined weakens the ability of the chaperonin subunit to form stable hexadecamers and as a consequence, the chaperoning functions of the complex are impaired. (frontiersin.org)
  • coli
  • All vectors in the CheckMate™ Mammalian Two-Hybrid System confer ampicillin resistance and are compatible with E. coli. (oyun-oyna.tk)
  • actin
  • 2) We have demonstrated that the fluorescence intensity of rhodamine-labeled single actin filaments changes depending on the applied load, implying that the local structure of actin is distorted. (nii.ac.jp)
  • Actin is made of two strings of beads wound round each other and is one of the three main parts of the cytoskeleton of eukaryotic cells. (wikipedia.org)
  • complexes
  • Among these, the chaperonins are 1-MDa ring-shaped oligomeric complexes that bind unfolded polypeptides and promote their folding within an isolated chamber in an ATP-dependent manner. (osti.gov)
  • In this project, we studied physicochemical relationships between structural fluctuations and functional expression of the chaperonin complexes. (nii.ac.jp)
  • complex
  • Crystals of the two forms were grown by the vapor batch crystallization method at 295 K. Crystals of the Ch -CPN/AMPPNP complex diffracted to 3.0 Å resolution and belonged to the space group P 422, with unit-cell parameters a = b = 186.166, c = 160.742 Å. (springer.com)
  • Crystals of the Ch -CPN/ADP complex diffracted to 4.0 Å resolution and belonged to the space group P 42 1 2, with unit-cell parameters a = b = 209.780, c = 169.813Å. (springer.com)
  • enzyme
  • Chaperonin overexpression promotes genetic variation and enzyme evolution. (semanticscholar.org)
  • In contrast, some of its derivatives display binding activity to the enzyme topoisomerase II during the late S and early G2 stage. (wikipedia.org)
  • This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. (wikipedia.org)
  • The systematic name of this enzyme class is 2-methylpropanoyl-CoA:enzyme-N6-(dihydrolipoyl)lysine S-(2-methylpropanoyl)transferase. (wikipedia.org)
  • Other names in common use include dihydrolipoyl transacylase, enzyme-dihydrolipoyllysine:2-methylpropanoyl-CoA, S-(2-methylpropanoyl)transferase, 2-methylpropanoyl-CoA:enzyme-6-N-(dihydrolipoyl)lysine, and S-(2-methylpropanoyl)transferase. (wikipedia.org)
  • The two known serotypes can be distinguished biochemically by differences in enzyme activity. (wikipedia.org)
  • bound
  • Here, we report the crystallization of both the AMPPNP (an ATP analogue) and ADP bound forms of a novel CPN, classified as belonging to a third Group, recently discovered in the extreme thermophile Carboxydothermus hydrogenoformans . (springer.com)
  • bacterial
  • La prévalence de la résistance acquise peut varier en fonction de la géographie et du temps pour certaines espèces.A combined yeast/bacterial two-hybrid system is disclosed. (oyun-oyna.tk)
  • function
  • However, recent work is beginning to shed light on key aspects of chaperonin function and how their unique properties underlie their contribution to maintaining cellular proteostasis. (osti.gov)
  • found
  • In 1995, S. shiloi was found in fact to be beta-hemolytic, and after DNA-DNA hybridization techniques with the ATCC type S. iniae and recalculation of the G+C% content, was reclassified by the same group as a junior synonym of S. iniae. (wikipedia.org)
  • bind
  • For example, nascent polypeptide chain coming out from ribosome will first bind to Hsp70/Hsp90 which will help attain a quasinative structure and then will be transferred to chaperonin CCT for its final folding [ 14 , 15 ]. (pubmedcentralcanada.ca)
  • genes
  • Pichia pastoris has two alcohol oxidase genes, Aox1 and Aox2, which have a strongly inducible promoter. (wikipedia.org)
  • Therefore, some key genes overlap with other disease groups. (wikipedia.org)
  • species
  • It cannot be grouped by the Lancefield antigen method typically used to categorize Streptococcus species. (wikipedia.org)
  • Several groups have used 16S rDNA sequencing to identify S. iniae isolates, and while it can differentiate this species from other related species, such as S. porcinus and S. uberis, 16S sequencing cannot be used to differentiate between strains of S. iniae. (wikipedia.org)
  • Cell
  • The generation of CD8 T cell responses occurs in two phases, both of which critically involve the process of Ag presentation. (jimmunol.org)
  • To monitor what cells are producing, the immune system has evolved mechanisms to sample the library of peptides produced by cellular catabolism and display them on the cell surface for scrutiny by CD8 T cells ( 2 ). (jimmunol.org)
  • However Pichia has two main advantages over S. cerevisiae in laboratory and industrial settings: Firstly, Pichia, as mentioned above, is a methylotroph, meaning it can grow with the simple alcohol methanol as its only source of energy - Pichia can easily be grown in cell suspension in reasonably strong methanol solutions that would kill most other micro-organisms, a system that is cheap to set up and maintain. (wikipedia.org)
  • Thus
  • Ribotyping was used in 1997 to differentiate between Israeli and American strains, thus ruling out the possibility of an epidemiological link between outbreaks in the two countries. (wikipedia.org)
  • However
  • However, a micrograph often contains particles in multiple different orientations and/or conformations, and so to get more representative image averages, a method is required to group similar particle images together into multiple sets. (wikipedia.org)
  • various
  • Depending on the sample or the desired results, various steps of two- or three-dimensional processing can be done. (wikipedia.org)
  • addition
  • In addition, the stereochemistry at C-2 and C-3 configures a trans-lactone, which has more activity than the cis counterpart. (wikipedia.org)
  • form
  • In 2006 a research group has managed to create a strain that produces Erythropoietin in its normal glycosylation form. (wikipedia.org)
  • aggregation
  • To examine the role of BFP in EPEC aggregation and dispersal, we examined HEp-2 cell adhesion of strain E2348/69 and defined E2348/69 mutants by immunofluorescence and immunoelectron microscopy. (antievolution.org)
  • 1997
  • Ribotyping was used in 1997 to differentiate between Israeli and American strains, thus ruling out the possibility of an epidemiological link between outbreaks in the two countries. (wikipedia.org)
  • Heat
  • In 1962, Italian geneticist Ferruccio Ritossa reported that heat and the metabolic uncoupler 2,4-dinitrophenol induced a characteristic pattern of "puffing" in the chromosomes of Drosophila. (wikipedia.org)
  • utilize
  • Although 4/5ths of earth's atmosphere is comprised of nitrogen, the ability to utilize atmospheric nitrogen is restricted to a few groups of prokaryotes that are able to covert atmospheric nitrogen to ammonia and, in the case of the legume symbiosis, make some of this available to plants. (intechopen.com)
  • prokaryotes
  • For much of the 20th century, prokaryotes were regarded as a single group of organisms and classified based on their biochemistry, morphology and metabolism. (wikipedia.org)
  • Woese argued that this group of prokaryotes is a fundamentally different sort of life. (wikipedia.org)
  • nitrogen
  • Biological nitrogen fixation (BNF) is one of the most important phenomena occurring in nature, only exceeded by photosynthesis [ 1 , 2 ]. (intechopen.com)
  • known
  • Today, they are known to be a large and diverse group of organisms that are widely distributed in nature and are common in all habitats. (wikipedia.org)
  • however
  • however, 2-DE has many drawbacks. (usf.edu)
  • However, a micrograph often contains particles in multiple different orientations and/or conformations, and so to get more representative image averages, a method is required to group similar particle images together into multiple sets. (wikipedia.org)
  • type
  • Beevers type ball and spoke model of hemoglobin at 1 cm/2 Å, available from Miramodus.Com . (proteopedia.org)
  • chain
  • Chaperonins are a special class of chaperones that promote native state folding by cyclically encapsulating the peptide chain. (wikipedia.org)
  • active
  • In 1979, inhibition by phenylglyoxal determined that a phosphate group from either propionyl-CoA or ATP reacts with an essential arginine residue in the active site during catalysis. (wikipedia.org)
  • A number of models of chaperonin action have been proposed, which generally focus on two (not mutually exclusive) roles of chaperonin interior: passive and active. (wikipedia.org)
  • Once the tubulins are in contact with the chaperonin, the prefoldin automatically lets go and leaves the active site, due to its high affinity for the chaperonin molecule. (wikipedia.org)
  • models
  • From 1958 into the 1960's, these included brass wireframe models , facilitated by the Richards optical comparator , and smaller ball and spoke models [2] . (proteopedia.org)
  • The models were built and supported within a forest of 2,500 vertical rods arranged to fill a cube six feet (2 meters) on a side. (proteopedia.org)